Olympus CKX41 User Manual

INVERTED MICROSCOPE

CKX41/CKX31

UNIVERSAL

INFINITY SYSTEM

A new advance in routine inspections

1

CKX series microscopes make cell checking quicker and easier than ever —
because they are simple to operate, require minimal optical adjustments and
capture the best possible observation images with outstanding efficiency.
Use of a tilting observation tube, which allows the user to make observations while standing,
is an especially convenient feature.
In addition, the compact design allows installation right beside the incubator,
improving safety and saving time spent on carrying specimens elsewhere for observation.
Multiple observation methods are supported, and the range of applications has been further increased.
The CKX series: designed to make routine cell checking quicker, easier and more efficient.

Phase-contrast

Phase-contrast

Relief contrast

Relief contrast

Incorporation of advanced UIS optics

ensures the highest level of clarity for
cell checking applications.

CKX41

A binocular tilting tube, which
allows observation while
standing, and a trinocular
observation tube which is
compatible with various video
cameras are available.
Numerous accessories make it
easy to upgrade the CKX41
according to need.

CKX31

Standard microscope for cell
culture observation with fixed
binocular tube.

PHC objective minimally affected by
surface tension around the container edge

The PHC objective is minimally affected by surface tension of the
culture fluid, which can compromise clarity at the image periphery.
Easier multi-well observation is one of several benefits. Combined
with the improved flatness
resulting from the use
of UIS optics, this
feature facilitates
clear observation of
cells even at the
edge of the container.

PHC objective

2

Fluorescence

Fluorescence

Clear up to the edge of the view field.
Flatness improved for 10 to 15%

Clear, wide-ranging phase-contrast observation.
Ideal phase plate for cell culture.

Clear cell observation right to the edge of the container.
PHC objective virtually unaffected by surface tension.

Enhanced clarity makes it easier to
observe cell activity status

Incorporation of the world-renowned Olympus UIS optical system
in the CKX series makes a dramatic improvement in flatness for
10 to 15% and enables very clear, high contrast images to be
obtained up to the edge of the field view.

Adoption of a UIS phase plate optimized for
cell culture observation provides higher image contrast

The UIS phase plate adjusts the contrast according to the thickness
of the cell culture, producing higher contrast images than those
obtainable from conventional systems.

Low magnification UIS video adapters can be used for

0.25X, 0.35X and 0.5X magnifications, enabling clear
display of the complete cell on the monitor.

The UIS optics provide unsurpassed optical performance

The UIS optical system combines with the simplicity of the light path
and the imaging excellence of a relay lenses to maintain the highest
possible image intensity and to arrive at the highest optical
correction level. Thanks to this feature, the UIS infinity-corrected
optical system deliver unsurpassed resolution and contrast.
In addition to improved image quality, the maximum field of view
has been extended to F.N. 22. This allows the use of various
observation tubes provided for upright microscopes (patented,
CKX41 only).

UIS infinity-corrected optical system

The advanced Olympus UIS optical system maximized the advantages of infinity
correction. Light travels through the body tube as parallel rays as it passes through
the objective. These are focused by the tube lens to form a completely aberration­free intermediate image. Attachments can be added between the objective and the
built-in tube lens in the observation tube without any magnification factor alterations
to total magnification. Additional correction lenses are not required The UIS optical
system delivers optimum image quality with any configuration.

Standard field number 20

Image capturing area 22

Maximum field number 22

General objective

Half mirror

Objective

Parallel light

Illumination light

Tube lens

Image forming ray

Primary focus plane

Reflected ray

3

4

Just set the specimen on the stage and observe
immediately while standing, with no optical adjustments.

Pre-centered phase-contrast slider for quick,
adjustment-free observation

With the pre-centered phase-contrast slider, there is no need to
repeat centering each time the objective magnification is changed.
Likewise, ring slit exchange when swapping between objectives is
also unnecessary, since the ring slit is standardized for 10X, 20X
and 40X magnifications. The CKX series makes phase-contrast
observations quick and easy, without optical adjustments, for much
more efficient performance of routine tasks. Since the phase plate
transmission rates are dictated by the objective in use, the
brightness remains constant
even when the magnification
is changed.

*The IX2-SL with centering mechanism
enables maximized phase-contrast
performance with precise optical
adjustments.

Slim, compact design takes up minimum laboratory space

The slim, compact design minimizes the depth of the microscope,
allowing easy installation right beside the incubator or clean bench.
Specimens can be taken and immediately set on the microscope
stage for quick, efficient inspection.

Easy observation at the clean bench

The tilting binocular tube combination makes it unnecessary for
researchers to adopt uncomfortable postures like lowering their face
to the inside of the clean bench. Simply observe down the tube
while working, and raise the tube afterwards: this method is much
more convenient, since the clean bench can be opened/closed
without moving the microscope.

Tilting binocular tube for observation while standing

The binocular tube has a 30-60 degree tilting mechanism to let the
researcher make observations while standing. The specimen is
removed from the incubator and placed straight onto the
microscope stage, so that cell checking can be done immediately.
"Quick observation, quick return"— that’s the new style of routine
inspection made possible by Olympus.

Empty slot10X, 20X, 40X4X

10X 20X 40X

Fluorescence observation system with
tree-position allows the use of U excitation.

Adopted renowned UIS optics, this system provides
B and G excitations and optionally U excitation as well.
All three can be provided simultaneously, with quick,
easy exchange among them. Many different UIS filters
can also be installed, according to requirements,
enabling this system to meet a wide range of
fluorescence observations needs.
Also, thanks to newly developed fluorescent illuminator,
which secured UV transmission rate, and a variety of

UIS fluorescent
objectives, clear
and high contrast
fluorescent
images are
provided.

5

High-resolution relief contrast with N.A.0.5:
thick specimens like egg cells can be
observed in three dimensions

Olympus' relief contrast maintains the same shadow
direction even if the magnification is changed.
The two-stage exchange slider uses a common
aperture for 20X and 40X magnifications, so exchange
between 10X/20X and 10X/40X is performed quickly.
The same feature enables easy optical adjustment:
by simply using the lever, the contrast can be
changed while centering is being performed.

Suitable for blood cell plates and
other kinds of micro-plate

The mechanical stage offers
excellent inspection performance
with blood cell plates or other micro­plates.

Glass stage insert plate and
warm plate

Also included are a glass stage
insert plate, for simple confirmation
of the objective in use, and a warm
plate for easy temperature control
(CKX41 only).

Relief contrast observations

Fluorescence observations

Accessories

Cost-effective digital imaging

Mounting the trinocular tube on the CKX41 makes it
possible to attach the DP12
digital camera or
different types of
video cameras.

CKX41+DP12

Vertical installation avoids interference with
manipulators

Vertical installation helps the user to avoid accidentally
touching the manipulators while operating the slider or
making optical adjustments.

CKX41/CKX31 dimensions (unit: mm)

Specifications are subject to change without any obligation on the part of the manufacturer.

ISO9001

Certification

Design and production

adheres to ISO9001

international quality standard.

ISO 9001

Accredited by the
Dutch Council for

Accreditation

ISO14001

Design and production at the Olympus

Optical Co. Ltd. Ina Plant conforms with

ISO14001 specifications for

environmental management systems.

Certification

ISO 14001

008

UKAS

ENVIRONMENTAL

MANAGEMENT

Item CKX41 CKX31
Optical system UIS (Universal Infinity-corrected) optical system
Focus Vertical nosepiece movement (stage is fixed),

coaxial coarse and fine focus with tension adjustment mechanism, roller slide mechanism,
stroke: 7mm up and 2mm down from focus position which is 1mm above the stage,

stroke per rotation: 39.6mm (coarse), 0.2mm (fine)
Revolving nosepiece Quadruple
Stage Plain stage 160mm(L) x 250mm(W), stage clips attachable

Exchangeable insert plate (ø25mm opening) incorporated ø35 petri dish holder stage

incorporated

Mechanical stage Right-hand low drive coaxial controls,

stage movement: X=120mm, Y=78mm, with three dish/sample holders

Substage 70(L) X180(W)mm

Illumination Light source 6V,30W halogen lamp, lamp socket (U-LS30-3-2),
system built-in frosted and heat absorbing filters, detachable illuminator

Filter holder Insert up to 11mm thick with ø45mm filter, detachable
Aperture diaphragm Lever type, range: from minimum ø3mm to maximum ø44mm

Slider insertion With phase slider pocket and built-in slider position click stop mechanism
Condenser Detachable ultra-long working distance condenser (N.A. 0.3, W.D. 72mm)
Contrast slider •Pre-centered phase-contrast: 4X, 10X/20X/40X, empty slot

•Centerable phase-contrast: 4X, 10X/20X, empty slot (40X optional, pre-centered)

•Centerable for Relief Contrast: 10X, 20X, 40X

Observation Binocular tube U-CBI30-2: inclined 30°, interpupillary distance range: 48-75mm,
tube diopter adjustment by helicoid on left sleeve (F.N. 20)

U-BI30-2: inclined 30°, interpupillary distance range: 48-75mm,
diopter adjustment by helicoid on left sleeve (F.N. 22)

Trinocular tube U-CTR30-2: inclined 30°, ring dovetail attachment,

interpupillary distance range: 48-75mm, tube length
and diopter adjustment by helicoid on left sleeve
Observation optical path: 50(binocular)/50(video port) (F.N. 20)

U-TR30-2: inclined 30°, ring dovetail attachment,
interpupillary distance range: 48-75mm, tube length
and diopter adjustment by helicoid on left sleeve
Observation optical path: 50(binocular)/50(video port) (F.N. 22)

Tilting binocular CKX-TBI: variable inclination angles from 30° to 60°,

tube interpupillary distance range: 50-76mm,

diopter adjustment by helicoid on right sleeve (F.N. 20)
UTBI-3: variable inclination angles from 5° to 35°, circular

mounting dovetail attachment, interpupillary distance range:
50-76mm, diopter adjustment by helicoid on right sleeve. (F.N. 22)

Fluorescent illuminator Detachable illuminator,

switchable slide (3-position: B excitation, G excitation, empty slot or U excitation)

FL light source 50W Hg

FL light shutter Available

FL field stop Available

FL cubes 2 cubes (B & G), option U

(cubes are not compartible with UIS. Filter and dichroic mirror size are same as UIS)

Filter 1 filter
Eyepiece For U-CBI30-2/U-CTR30-2/CKX-TBI: WHB10X/WHB10X-H (F.N. 20) 10X (F.N.: 20)

For U-BI30-2/U-TR30-2/U/TBI3: WH10X/WH10X-H/35WH10X/

PWH10X (F.N. 22)

Power supply Continuous intensity adjustment,

built-in voltage changeover switch (100/120V, 220/240V), frequency 50/60Hz

CKX41/CKX31 specifications

—

Objective N.A. W.D.(mm) Remarks
PLC4X 0.10 22.0
PLC10X 0.25 10.5
ACH20X 0.40 3.0
LCPLFL40X 0.60 2.6
UPLFL4XPH 0.13 17.0 PHL (for use with IX2-SL)
CPL10XPH 0.25 9.8 PHC (for use with IX2-SL)
PL10XPH 0.25 10.5 PH1 (for use with IX2-SL)
LCACH20XPH 0.40 3.2 PHC (for use with IX2-SL)
LCPLFL20XPH 0.40 6.9 PH1 (for use with IX2-SL)
LCACH40X2PH 0.55 2.2 PH2 (for use with IX2-SL)
LCPLFL40XPH 0.6 2.6 PH2 (for use with IX2-SL)
UPLFL4XPHP* 0.13 17.0 For use with IX2-SLP
CACH10XPHP* 0.25 8.8 For use with IX2-SLP
LCACH20XPHP* 0.40 3.2 For use with IX2-SLP
LCACH40XPHP* 0.55 2.2 For use with IX2-SLP
CPL10XRC 0.25 9.5 For use with CKX-RC
LCACH20XRC 0.4 2.8 For use with CKX-RC
LCACH40XRC 0.55 1.9 For use with CKX-RC
UPLFL4X 0.13 17 U,B,G
UPLFL10X 0.3 10 U,B,G
UPLFL20X 0.5 1.6 U,B,G
LCPLFL20X 0.4 6.9 B,G
LUCPLFL40X 0.6 2.7 - 4.0

Objective lens specifications

For brightfield

For phase-contrast

For RC

For FL

* Pre-centering objective

Fixed binocular tube, inclined
45°, interpupillary distance range:
48-75mm, diopter adjustment by
helicoid on right sleeve

237.6

195

393.1

515.5

473.7

370.5

364

194.5

237.6

473.2

380.5

486.6

370.5

364