Metrohm 754 User Manual
CH-9101 Herisau/Switzerland
Tel. ++41 71 353 85 85
Fax ++41 71 353 89 01
Internet www.metrohm.ch
E-Mail info@metrohm.ch
754 Dialysis Unit
Dialysis Unit754
POWER
REMOTE PUMP
ON ON
OFFOFF
MetrohmMetrohm
8.754.1003 Instructions for Use
19.01.98 / dö
Table of contents
Table of contents
1 Introduction............................................................................................1
1.1 Instrument description.............................................................................1
1.2 Parts and controls..................................................................................... 2
1.3 Information about the Instructions for Use..........................................4
1.3.1 Organization ..................................................................................4
1.3.2 Notation and pictograms............................................................... 4
1.4 Support documentation...........................................................................5
1.5 Safety notes ...............................................................................................6
1.5.1 Electrical safety..............................................................................6
1.5.2 General precautionary rules.......................................................... 6
2 Installation..............................................................................................7
2.1 Setting up the instrument........................................................................ 7
2.1.1 Packaging......................................................................................7
2.1.2 Check ............................................................................................7
2.1.3 Location......................................................................................... 7
2.1.4 Arrangement of the instruments....................................................7
2.2 Mains connection......................................................................................8
2.2.1 Setting the mains voltage..............................................................8
2.2.2 Fuses.............................................................................................9
2.2.3 Mains cable and mains connection ..............................................9
2.2.4 Switching the instrument on/off..................................................... 9
2.3 Attaching the accessories.....................................................................10
2.3.1 Electrical connection to the 732 IC Detector............................... 10
2.3.2 Attaching the accessories to the
733.0020 IC Separation Center................................................... 10
2.3.3 Attaching the accessories to the 754 Dialysis Unit.....................13
2.3.4 Assembling the dialysis cell ........................................................15
2.3.5 Connecting the dialysis cell ........................................................17
2.3.6 Attaching the accessories to the waste bottle ............................19
2.3.7 Conditioning the dialysis system ................................................20
754 Dialysis Unit
3 Operation................................................................................................21
3.1 Manual operation.....................................................................................21
3.2 Operation via 732 IC Detector...............................................................22
3.3 The dialysis procedure...........................................................................23
3.3.1 Dialysis flow chart........................................................................ 23
3.3.2 Programming with the 732 IC Detector.......................................24
3.3.3 Setting the rinsing time................................................................ 25
3.3.4 Setting the transfer time ..............................................................25
3.3.5 Setting the dialysis time...............................................................26
3.3.6 Setting the program time............................................................. 27
3.3.7 Dialysis procedure....................................................................... 27
I
Table of contents
4 Malfunctions – Maintenance..........................................29
5 Appendix .................................................................................................35
4.1 Malfunctions and their rectification.....................................................29
4.2 Maintenance and servicing ................................................................... 30
4.2.1 Handling dialysis membranes .................................................... 30
4.2.2 Care of the unit............................................................................ 30
4.2.3 Maintenance by Metrohm service............................................... 30
4.2.4 Shutdown ....................................................................................31
4.2.5 Replacing the pump tubing ........................................................ 31
4.2.6 Replacing the dialysis membrane .............................................. 32
5.1 Technical data ......................................................................................... 35
5.2 Standard equipment............................................................................... 37
5.3 Optional accessories.............................................................................. 39
5.4 Warranty and conformity....................................................................... 40
5.4.1 Warranty ...................................................................................... 40
5.4.2 EU Declaration of conformity ...................................................... 41
5.4.3 Certificate of conformity and system validation.......................... 42
5.5 Index.......................................................................................................... 43
List of figures
Fig. 1: Front of the 754 Dialysis Unit ............................................................................ 2
Fig. 2: Rear of the 754 Dialysis Unit ............................................................................. 3
Fig. 3: Setting the mains voltage.................................................................................. 9
Fig. 4: Connection of 754 Dialysis Unit to 732 IC Detector........................................ 10
Fig. 5: Interior of the 733.0020 IC Separation Center................................................. 12
Fig. 6: Installing pump tubings................................................................................... 14
Fig. 7: Assembling the dialysis cell ............................................................................ 16
Fig. 8: Diagram of tubing connections ...................................................................... 18
II
754 Dialysis Unit
1 Introduction
1.1 Instrument description
The 754 Dialysis Unit is a unit for on-line sample preparation in ion
chromatography permitting the use of automatic sample dialysis directly before sample injection. It consists of a dual-channel peristaltic
pump for conveying the sample and acceptor solutions, and the actual
dialysis cell in which the ions from the flowing sample solution are enriched in the resting acceptor solution and then injected directly into the
IC system. This special Stopped-Flow Method (for which Metrohm
has applied for a patent) means that 100% of the sample concentration
can be achieved in the acceptor solution, allowing easy calibration with
external standards.
1.1 Instrument description
The 754 Dialysis Unit can be operated manually, via the ON/OFF
switch, or by remote control, via the remote interface.
Valve B, which is built into the 733.0020 IC Separation Center and
controlled by the 732 IC Detector, is required to operate the 754 Dialysis Unit. For this variant, which is described in these Instructions for
Use, the following units are required:
Operation without suppressor
2.732.0010 IC Detector
2.733.0020 IC Separation Center
2.709.0010 IC Pump
2.754.0010 Dialysis Unit
As an alternative to valve B, which is built into the IC Separation Center,
an external valve controlled via the remote output of the 732 IC De-
tector can also be used. This allows the 754 Dialysis Unit to be operated in combination with the following units:
Operation with suppressor
2.732.0010 IC Detector
2.733.0030 IC Separation Center
2.709.0010 IC Pump
2.752.0010 Pump Unit
2.754.0010 Dialysis Unit
Operation with suppressor
2.732.0010 IC Detector
2.733.0020 IC Separation Center
2.709.0010 IC Pump
2.754.0010 Dialysis Unit
2.753.0010 Suppressor Module
External valve
754 Dialysis Unit
More information about this variant is available from Metrohm on request.
1
1 Introduction
1.2 Parts and controls
33 44 55
Dialysis Unit754
POWER
REMOTE PUMP
ON ON
OFFOFF
MetrohmMetrohm
22 11 77991010 88 66
Fig. 1: Front of the 754 Dialysis Unit (without dialysis cell)
11 Pump ON/OFF
On/Off switch for the pump
(manual operation mode)
22 Remote ON/OFF
On/Off switch for remote control
of the pump
33 Power on lamp
Lights up when instrument is
switched on
44 Tubing cartridge
For 6.1826.0X0 pump tubing
55 Contact pressure lever
For adjusting the contact pressure
66 Cell holder
For holding the dialysis cell
77 Holding clamp
For locking the tubing cartridge into
place
88 Snap-action lever
For releasing the tubing cartridge
99 Pump drive
Roller head with contact rollers
1010 Mounting pin
For attaching the tubing cartridge
2
754 Dialysis Unit
1.2 Parts and controls
1111
WARNING - Fire Hazard -
For continued protection replace only
with the same type and rating of fuse
f=50-60 Hz
S=12 VA
115V: 110-120V:
230V: 220-240V:
Type 1.754.0010 Nr.
Fuse
0,2A(T)
0,1A(T)
S
141413131212
Remote
Pump
Made by Metrohm Herisau Switzerland
1616 1515
Fig. 2: Rear of the 754 Dialysis Unit
1111 Fuse holder
Changing the fuses, see section 2.2
1212 Voltage selection insert with
display of voltage
1313 Mains switch
For switching the instrument on/off:
I = ON 0 = OFF
1414 Serial number
1515 Remote interface
Remote I/O lines for connecting
external devices (e.g. 732 IC Detector)
1616 Mains connection plug
Mains connection, see section 2.2
754 Dialysis Unit
3
1 Introduction
1.3 Information about the Instructions for Use
Please read through these Instructions for Use carefully before operating the 754 Dialysis Unit. The Instructions for Use contain information and warnings to which the user must pay attention in order to
assure safe operation of the instrument.
1.3.1 Organization
These 8.754.1003 Instructions for Use for the 754 Dialysis Unit provide a comprehensive overview of the installation, startup procedure,
operation, fault rectification and technical specifications of this instrument. The Instructions for Use are organized as follows:
Section 1 Introduction
Description of the instrument, parts and controls,
safety notes
Section 2 Installation
Setting up, mains connection, attachment of
accessories, connection to IC system
Section 3 Operation
Manual operation, operation via 732 IC Detector,
dialysis procedure
Section 4 Faults – Maintenance
Fault rectification, maintenance
Section 5 Appendix
Technical data, standard equipment, options, warranty,
declarations of conformity, index
To find the information you require about the instruments please use
either the Table of contents or the Index at the back.
1.3.2 Notation and pictograms
The following notations and pictograms (symbols) are used in these Instructions for Use:
<PUMP> Switch or key
1515 Part or control of 754
8989 Part or control of 732/733
Range Parameter or entry value
at 732 IC Detector
4
754 Dialysis Unit
1.4 Support documentation
Hazard
This symbol draws attention to a
possible danger to life or injury if
the associated directions are not
followed correctly.
Warning
This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed correctly.
Caution
This symbol marks important information. Read these directions
before continuing.
1.4 Support documentation
Application Bulletins
The «Application Bulletin» is a collection of analytical methods, application examples and literature references. Of Metrohm's approximately
200 Application Bulletins, 34 currently refer to ion chromatography. All
these Application Bulletins are available on request free of charge from
your Metrohm supplier.
You will find an updated list of the Application Bulletins at any time under «http://www.metrohm.ch».
Application Notes
The «Application Notes» present application information in concentrated form, i.e. on maximum 2 pages. There are currently 88 Application Notes (in English) in the field of ion chromatography. You can order
these free of charge from your Metrohm supplier or view them in the
Internet under «http://www.metrohm.ch» and copy them from there.
Comment
This symbol marks additional information and tips.
754 Dialysis Unit
5
1 Introduction
1.5 Safety notes
1.5.1 Electrical safety
While electrical safety in the handling of the 754 Dialysis Unit is assured
in the context of the specifications IEC 1010-1 (protection class I, degree of protection IP40), the following points should be noted:
• Mains connection
Set the mains voltage and check the mains fuse and mains
connection in accordance with the instructions in section 2.2.
• Opening the 754 Dialysis Unit
To avoid all danger of coming into contact with live components do
not open the instrument or remove any parts when the 754 Dialysis
Unit is connected to the power supply. Always disconnect the instrument from all voltage sources before you open it and ensure that the
mains cable is disconnected from mains connection 1616 !
• Protection against static charges
Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 754 Dialysis Unit, you should earth yourself and any tools
you are using by touching an earthed object (e.g. housing of the
instrument or a radiator) to eliminate any static charges which exist.
1.5.2 General precautionary rules
• Handling of solvents
Check all pump tubings and all input and output leads periodically for
possible leaks. Follow the relevant instructions regarding the handling
of flammable and/or toxic solvents and their disposal.
• Regular exchange of pump tubings
Pump tubings constitute consumable material and must be replaced
from time to time (see section 4.4). Suitable measures must be taken
so that any leak which might occur in the pump tubing or connections
during unattended operation will cause no damage (placing the
instrument at the bottom, collection device for any liquid which may
leak out).
6
754 Dialysis Unit
2 Installation
2.1 Setting up the instrument
2.1.1 Packaging
The 754 Dialysis Unit is supplied together with the separately packed
accessories in special packagings containing shock-absorbing foam
linings designed to provide excellent protection. The actual instrument
is packed in an evacuated polyethylene bag to prevent the ingress of
dust. Please store all these special packagings as only they can assure
damage-free transport of the instrument.
2.1.2 Check
2.1 Setting up the instrument
After receipt, immediately check whether the shipment is complete and
undamaged (compare with delivery note and list of accessories in
section 5.2). In the case of transport damage, see instructions in
section 5.4.1 "Warranty".
2.1.3 Location
Position the instrument in the laboratory at a location convenient for
operation, free from vibrations and protected against a corrosive
atmosphere and contamination by chemicals.
2.1.4 Arrangement of the instruments
To make the connections with the tubing supplied, position the 754 Dialysis Unit immediately under the 733 IC Separation Center. The other
units (732 IC Detector, 709 IC Pump, 753 Suppressor Module) can be
arranged as required.
Take precautions to ensure that any leaks from pump tubings or
connections cannot cause more damage.
754 Dialysis Unit
7
2 Installation
2.2 Mains connection
Follow the instructions below for connecting to the power supply. If
the instrument is operated with the mains voltage set wrongly and/or
wrong mains fuse there is a danger of fire!
2.2.1 Setting the mains voltage
Before switching on the 754 Dialysis Unit for the first time, check that
the mains voltage set on the instrument (see Fig. 3) matches the local
mains voltage. If not, reset the mains voltage on the instrument as follows:
Disconnect mains cable
1
Disconnect mains cable from mains connection plug 1616 of the
754 Dialysis Unit.
Remove fuse holder
2
Using a screwdriver, loosen fuse holder 1111 and remove com-
pletely.
Change mains voltage
3
Completely remove voltage selector insert 1212 by hand, rotate it
through 180° and reinsert it. The required mains voltage (115 or
230 V) should now be visible from the front.
Check fuses
4
Carefully take both fuses out of fuse holder 1111 and check their
specifications:
100……120 V 0.2 A (slow-blow) Metrohm-No. U.600.0009
220……240 V 0.1 A (slow-blow) Metrohm-No. U.600.0006
Insert fuses
5
Change both fuses if necessary and reinsert in fuse holder 1111.
Install fuse holder
6
Push fuse holder 1111 back into the opening of the 754 Dialysis
Unit by hand until it clicks into place properly.
8
754 Dialysis Unit
2.2.2 Fuses
2.2 Mains connection
100 – 120 V220 – 240 V
1212
1111
230
115
1111 Fuse holder
1212 Voltage selection
insert with display
1313
of voltage
1313 Mains switch
1616
1616 Mains connection
plug
Fig. 3: Setting the mains voltage
Two fuses 0.2 A/slow-blow for 100…120 V or 0.1 A/slow-blow for
220…240 V are installed in the fuse holder 1111 of the 754 Dialysis Unit
as standard.
To eliminate any danger of fire, ensure that the instrument is never
operated with any other type of fuses!
To check or change fuses, proceed as described in section 2.2.1.
2.2.3 Mains cable and mains connection
Mains cable
The instrument is supplied with one of three mains cables
• 6.2122.020 with plug SEV 12 (Switzerland, …)
• 6.2122.040 with plug CEE(7), VII (Germany, …)
• 6.2133.070 with plug NEMA 5-15 (USA, …)
which are three-cored and fitted with a plug with an earthing pin. If a
different plug has to be fitted, the yellow/green lead (IEC standard)
must be connected to protective earth (protection class I).
Any break in the earthing inside or outside the instrument can make it
a hazard!
Mains connection
Plug the mains cable into mains connection plug 1616 of the 754 Dialysis
Unit (see Fig. 3).
2.2.4 Switching the instrument on/off
The 754 Dialysis Unit is switched on and off using mains switch 1313.
When the instrument is switched on, lamp 33 lights up.
754 Dialysis Unit
9
2 Installation
2.3 Attaching the accessories
2.3.1 Electrical connection to the 732 IC Detector
In order to allow remote operation of the pump via the 732 IC Detector,
connection 1515 of the 754 Dialysis Unit must be connected to the
"Remote" connection of the 732 IC Detector by means of the
6.2143.200 cable as per Fig. 4. The switch 22 <REMOTE> on the front
of the 754 Dialysis Unit must additionally be set to "ON" to permit remote operation.
If you wish to connect a second, additional unit (e.g. 752, 753, 754) to
the remote interface of the 732 IC Detector, connect the Dialysis Unit
754 using the optionally available 6.2143.220 cable instead of the
6.2143.200 cable.
732
754
6.2143.200
Fig. 4: Connection of 754 Dialysis Unit to 732 IC Detector
2.3.2 Attaching the accessories to the 733.0020 IC Separation
Center
The 2.733.0020 IC Separation Center comes ready to operate with two
IC columns. To operate it with the 754 Dialysis Unit, reassemble it as
follows (see Fig. 5 and Fig. 8):
Dismantle accessories
1
• Unscrew the two sample loops mounted on valve A 1818 and
valve B 2222.
• Loosen the rotary nipples screwed onto the interior side of
feedthrough 2222 and 2828, pull the two PTFE suction tubings
right out of the connections and unscrew from connection "1"
of valve A 1818 and valve B 2222.
• Remove the PEEK capillaries running from connection "2" of
valve A 1818 and valve B 2222 to feedthroughs 2121 and 2727.
10
754 Dialysis Unit
2.3 Attaching the accessories
Connect new feedthrough
2
• Using a wrench or a spanner, unscrew the nut on the interior
side of feedthrough 2727 and remove the feedthrough.
• Insert new 6.2756.000 tubing feedthrough in the hole and
retighten the nut.
Attach tubing to valve A
3
• Screw 6.1825.210 PEEK sample loop (20 µL) to connections
"3" and "6" of valve A 1818 using the enclosed 6.2744.010 PEEK
compression fittings.
• Screw PEEK capillary 1919 (6.1831.060; L =1 m) to connection
"2" of valve A 1818 using a 6.2744.010 PEEK compression fit-
ting. Pull the other end of the tubing to the back through the
rear panel opening 4141.
• Screw PEEK capillary 2020 (6.1831.040; L =15 cm) to connec-
tion "1" of valve A 18 18 using a 6.2744.010 PEEK compression
fitting.
Attach tubing to valve B
4
• Screw the PEEK capillary 2020 (6.1831.040; L =15 cm) at-
tached to connection "1" of valve A 1818 to connection "3" of
valve B 22 22 using a 6.2744.010 PEEK compression fitting.
• Screw PEEK capillary 2121 (6.1831.040; L =15 cm) to connec-
tions "1" and "2" of valve B 2222 using two 6.2744.010 PEEK
compression fittings.
• Screw PEEK capillary 2525 (6.1831.050; L =40 cm) to connec-
tion "4" of valve B 2222 using a 6.2744.010 PEEK compression
fitting and pull the other end to the front through feedthrough
2727.
• Screw PEEK capillary 2424 (6.1831.050; L =40 cm) to connec-
tion "5" of valve B 2222 using a 6.2744.010 PEEK compression
fitting and pull the other end to the front through feedthrough
2727.
• Screw PEEK capillary 2323 (6.1831.050; L =40 cm) to connec-
tion "6" of valve B 2222 using a 6.2744.010 PEEK compression
fitting and pull the other end to the front through feedthrough
2828.
Connecting to the 753 Suppressor Module (Option)
5
• Insert the suppressor block in the 733 IC Separation Center
as per the 753 Instructions for Use.
• Connect tubing to the IC column, the detector block and the
waste in the 733 IC Separation Center as per the 753 Instructions for Use.
• Pull the two inlet capillaries for H2SO4 and H2O to the front
through feedthrough 2727 and connect to the 753 Suppressor
Module pump tubings.
754 Dialysis Unit
11
2 Installation
1919
1818
20
21
22
23
24
1717
FILL INJECT
A B
STEP
FILL INJECT
4141
Fig. 5: Interior of the 733.0020 IC Separation Center
1717 PEEK sample loop
(6.1825.210; 20 µµL )
1818 Injection valve A 2525 PEEK capillary
1919 PEEK capillary
(6.1831.060; 1 m)
2424 PEEK capillary
(6.1831.050; 40 cm)
(6.1831.050; 40 cm)
2121 Connection for syringe
2727
25
28282121 2222
12
2020 PEEK capillary
2222 Feedthrough
(6.1831.040; 15 cm)
2121 PEEK capillary
2727 Feedthrough
(6.1831.040; 15 cm)
2222 Injection valve B 2828 Feedthrough
2323 PEEK capillary
4141 Rear panel opening
(6.1831.040; 15 cm)
754 Dialysis Unit
Loading...