Metrohm 754 User Manual

CH-9101 Herisau/Switzerland
Tel. ++41 71 353 85 85
Fax ++41 71 353 89 01
Internet www.metrohm.ch
E-Mail [e-mail]

754 Dialysis Unit

Dialysis Unit754

POWER

REMOTE PUMP

ON ON

OFFOFF

MetrohmMetrohm

8.754.1003 Instructions for Use

19.01.98 / dö

Table of contents

Table of contents

1 Introduction............................................................................................1

1.1 Instrument description.............................................................................1

1.2 Parts and controls..................................................................................... 2

1.3 Information about the Instructions for Use..........................................4

1.3.1 Organization ..................................................................................4

1.3.2 Notation and pictograms............................................................... 4

1.4 Support documentation...........................................................................5

1.5 Safety notes ...............................................................................................6

1.5.1 Electrical safety..............................................................................6

1.5.2 General precautionary rules.......................................................... 6

2 Installation..............................................................................................7

2.1 Setting up the instrument........................................................................ 7

2.1.1 Packaging......................................................................................7

2.1.2 Check ............................................................................................7

2.1.3 Location......................................................................................... 7

2.1.4 Arrangement of the instruments....................................................7

2.2 Mains connection......................................................................................8

2.2.1 Setting the mains voltage..............................................................8

2.2.2 Fuses.............................................................................................9

2.2.3 Mains cable and mains connection ..............................................9

2.2.4 Switching the instrument on/off..................................................... 9

2.3 Attaching the accessories.....................................................................10

2.3.1 Electrical connection to the 732 IC Detector............................... 10

2.3.2 Attaching the accessories to the

733.0020 IC Separation Center................................................... 10

2.3.3 Attaching the accessories to the 754 Dialysis Unit.....................13

2.3.4 Assembling the dialysis cell ........................................................15

2.3.5 Connecting the dialysis cell ........................................................17

2.3.6 Attaching the accessories to the waste bottle ............................19

2.3.7 Conditioning the dialysis system ................................................20

754 Dialysis Unit

3 Operation................................................................................................21

3.1 Manual operation.....................................................................................21

3.2 Operation via 732 IC Detector...............................................................22

3.3 The dialysis procedure...........................................................................23

3.3.1 Dialysis flow chart........................................................................ 23

3.3.2 Programming with the 732 IC Detector.......................................24

3.3.3 Setting the rinsing time................................................................ 25

3.3.4 Setting the transfer time ..............................................................25

3.3.5 Setting the dialysis time...............................................................26

3.3.6 Setting the program time............................................................. 27

3.3.7 Dialysis procedure....................................................................... 27

I

Table of contents

4 Malfunctions – Maintenance..........................................29

5 Appendix .................................................................................................35

4.1 Malfunctions and their rectification.....................................................29

4.2 Maintenance and servicing ................................................................... 30

4.2.1 Handling dialysis membranes .................................................... 30

4.2.2 Care of the unit............................................................................ 30

4.2.3 Maintenance by Metrohm service............................................... 30

4.2.4 Shutdown ....................................................................................31

4.2.5 Replacing the pump tubing ........................................................ 31

4.2.6 Replacing the dialysis membrane .............................................. 32

5.1 Technical data ......................................................................................... 35

5.2 Standard equipment............................................................................... 37

5.3 Optional accessories.............................................................................. 39

5.4 Warranty and conformity....................................................................... 40

5.4.1 Warranty ...................................................................................... 40

5.4.2 EU Declaration of conformity ...................................................... 41

5.4.3 Certificate of conformity and system validation.......................... 42

5.5 Index.......................................................................................................... 43

List of figures

Fig. 1: Front of the 754 Dialysis Unit ............................................................................ 2

Fig. 2: Rear of the 754 Dialysis Unit ............................................................................. 3

Fig. 3: Setting the mains voltage.................................................................................. 9

Fig. 4: Connection of 754 Dialysis Unit to 732 IC Detector........................................ 10

Fig. 5: Interior of the 733.0020 IC Separation Center................................................. 12

Fig. 6: Installing pump tubings................................................................................... 14

Fig. 7: Assembling the dialysis cell ............................................................................ 16

Fig. 8: Diagram of tubing connections ...................................................................... 18

II

754 Dialysis Unit

1 Introduction

1.1 Instrument description

The 754 Dialysis Unit is a unit for on-line sample preparation in ion
chromatography permitting the use of automatic sample dialysis di­rectly before sample injection. It consists of a dual-channel peristaltic
pump for conveying the sample and acceptor solutions, and the actual
dialysis cell in which the ions from the flowing sample solution are en­riched in the resting acceptor solution and then injected directly into the
IC system. This special Stopped-Flow Method (for which Metrohm
has applied for a patent) means that 100% of the sample concentration
can be achieved in the acceptor solution, allowing easy calibration with
external standards.

1.1 Instrument description

The 754 Dialysis Unit can be operated manually, via the ON/OFF
switch, or by remote control, via the remote interface.

Valve B, which is built into the 733.0020 IC Separation Center and
controlled by the 732 IC Detector, is required to operate the 754 Dialy­sis Unit. For this variant, which is described in these Instructions for
Use, the following units are required:

Operation without suppressor

2.732.0010 IC Detector

2.733.0020 IC Separation Center

2.709.0010 IC Pump

2.754.0010 Dialysis Unit

As an alternative to valve B, which is built into the IC Separation Center,
an external valve controlled via the remote output of the 732 IC De-
tector can also be used. This allows the 754 Dialysis Unit to be oper­ated in combination with the following units:

Operation with suppressor

2.732.0010 IC Detector

2.733.0030 IC Separation Center

2.709.0010 IC Pump

2.752.0010 Pump Unit

2.754.0010 Dialysis Unit

Operation with suppressor

2.732.0010 IC Detector

2.733.0020 IC Separation Center

2.709.0010 IC Pump

2.754.0010 Dialysis Unit

2.753.0010 Suppressor Module

External valve

754 Dialysis Unit

More information about this variant is available from Metrohm on re­quest.

1

1 Introduction

1.2 Parts and controls

33 44 55

Dialysis Unit754

POWER

REMOTE PUMP

ON ON

OFFOFF

MetrohmMetrohm

22 11 77991010 88 66

Fig. 1: Front of the 754 Dialysis Unit (without dialysis cell)

11 Pump ON/OFF

On/Off switch for the pump
(manual operation mode)

22 Remote ON/OFF

On/Off switch for remote control
of the pump

33 Power on lamp

Lights up when instrument is
switched on

44 Tubing cartridge

For 6.1826.0X0 pump tubing

55 Contact pressure lever

For adjusting the contact pres­sure

66 Cell holder

For holding the dialysis cell

77 Holding clamp

For locking the tubing cartridge into
place

88 Snap-action lever

For releasing the tubing cartridge

99 Pump drive

Roller head with contact rollers

1010 Mounting pin

For attaching the tubing cartridge

2

754 Dialysis Unit

1.2 Parts and controls

1111

WARNING - Fire Hazard -

For continued protection replace only
with the same type and rating of fuse

f=50-60 Hz
S=12 VA

115V: 110-120V:
230V: 220-240V:

Type 1.754.0010 Nr.

Fuse
0,2A(T)
0,1A(T)

S

141413131212

Remote

Pump

Made by Metrohm Herisau Switzerland

1616 1515

Fig. 2: Rear of the 754 Dialysis Unit

1111 Fuse holder

Changing the fuses, see section 2.2

1212 Voltage selection insert with

display of voltage

1313 Mains switch

For switching the instrument on/off:

I = ON 0 = OFF

1414 Serial number

1515 Remote interface

Remote I/O lines for connecting
external devices (e.g. 732 IC De­tector)

1616 Mains connection plug

Mains connection, see section 2.2

754 Dialysis Unit

3

1 Introduction

1.3 Information about the Instructions for Use

Please read through these Instructions for Use carefully before oper­ating the 754 Dialysis Unit. The Instructions for Use contain informa­tion and warnings to which the user must pay attention in order to
assure safe operation of the instrument.

1.3.1 Organization

These 8.754.1003 Instructions for Use for the 754 Dialysis Unit pro­vide a comprehensive overview of the installation, startup procedure,
operation, fault rectification and technical specifications of this instru­ment. The Instructions for Use are organized as follows:

Section 1 Introduction

Description of the instrument, parts and controls,
safety notes

Section 2 Installation

Setting up, mains connection, attachment of
accessories, connection to IC system

Section 3 Operation

Manual operation, operation via 732 IC Detector,
dialysis procedure

Section 4 Faults – Maintenance

Fault rectification, maintenance

Section 5 Appendix

Technical data, standard equipment, options, warranty,
declarations of conformity, index

To find the information you require about the instruments please use
either the Table of contents or the Index at the back.

1.3.2 Notation and pictograms

The following notations and pictograms (symbols) are used in these In­structions for Use:

<PUMP> Switch or key

1515 Part or control of 754

8989 Part or control of 732/733

Range Parameter or entry value

at 732 IC Detector

4

754 Dialysis Unit

1.4 Support documentation

Hazard

This symbol draws attention to a
possible danger to life or injury if
the associated directions are not
followed correctly.

Warning

This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed cor­rectly.

Caution

This symbol marks important infor­mation. Read these directions
before continuing.

1.4 Support documentation

Application Bulletins

The «Application Bulletin» is a collection of analytical methods, applica­tion examples and literature references. Of Metrohm's approximately
200 Application Bulletins, 34 currently refer to ion chromatography. All
these Application Bulletins are available on request free of charge from
your Metrohm supplier.

You will find an updated list of the Application Bulletins at any time un­der «http://www.metrohm.ch».

Application Notes

The «Application Notes» present application information in concen­trated form, i.e. on maximum 2 pages. There are currently 88 Applica­tion Notes (in English) in the field of ion chromatography. You can order
these free of charge from your Metrohm supplier or view them in the
Internet under «http://www.metrohm.ch» and copy them from there.

Comment

This symbol marks additional in­formation and tips.

754 Dialysis Unit

5

1 Introduction

1.5 Safety notes

1.5.1 Electrical safety

While electrical safety in the handling of the 754 Dialysis Unit is assured
in the context of the specifications IEC 1010-1 (protection class I, de­gree of protection IP40), the following points should be noted:

• Mains connection

Set the mains voltage and check the mains fuse and mains
connection in accordance with the instructions in section 2.2.

• Opening the 754 Dialysis Unit

To avoid all danger of coming into contact with live components do
not open the instrument or remove any parts when the 754 Dialysis
Unit is connected to the power supply. Always disconnect the instru­ment from all voltage sources before you open it and ensure that the
mains cable is disconnected from mains connection 1616 !

• Protection against static charges

Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 754 Dialysis Unit, you should earth yourself and any tools
you are using by touching an earthed object (e.g. housing of the
instrument or a radiator) to eliminate any static charges which exist.

1.5.2 General precautionary rules

• Handling of solvents

Check all pump tubings and all input and output leads periodically for
possible leaks. Follow the relevant instructions regarding the handling
of flammable and/or toxic solvents and their disposal.

• Regular exchange of pump tubings

Pump tubings constitute consumable material and must be replaced
from time to time (see section 4.4). Suitable measures must be taken
so that any leak which might occur in the pump tubing or connections
during unattended operation will cause no damage (placing the
instrument at the bottom, collection device for any liquid which may
leak out).

6

754 Dialysis Unit

2 Installation

2.1 Setting up the instrument

2.1.1 Packaging

The 754 Dialysis Unit is supplied together with the separately packed
accessories in special packagings containing shock-absorbing foam
linings designed to provide excellent protection. The actual instrument
is packed in an evacuated polyethylene bag to prevent the ingress of
dust. Please store all these special packagings as only they can assure
damage-free transport of the instrument.

2.1.2 Check

2.1 Setting up the instrument

After receipt, immediately check whether the shipment is complete and
undamaged (compare with delivery note and list of accessories in

section 5.2). In the case of transport damage, see instructions in
section 5.4.1 "Warranty".

2.1.3 Location

Position the instrument in the laboratory at a location convenient for
operation, free from vibrations and protected against a corrosive
atmosphere and contamination by chemicals.

2.1.4 Arrangement of the instruments

To make the connections with the tubing supplied, position the 754 Di­alysis Unit immediately under the 733 IC Separation Center. The other
units (732 IC Detector, 709 IC Pump, 753 Suppressor Module) can be
arranged as required.

Take precautions to ensure that any leaks from pump tubings or
connections cannot cause more damage.

754 Dialysis Unit

7

2 Installation

2.2 Mains connection

Follow the instructions below for connecting to the power supply. If
the instrument is operated with the mains voltage set wrongly and/or
wrong mains fuse there is a danger of fire!

2.2.1 Setting the mains voltage

Before switching on the 754 Dialysis Unit for the first time, check that
the mains voltage set on the instrument (see Fig. 3) matches the local
mains voltage. If not, reset the mains voltage on the instrument as fol­lows:

Disconnect mains cable

1

Disconnect mains cable from mains connection plug 1616 of the
754 Dialysis Unit.

Remove fuse holder

2

Using a screwdriver, loosen fuse holder 1111 and remove com-
pletely.

Change mains voltage

3

Completely remove voltage selector insert 1212 by hand, rotate it
through 180° and reinsert it. The required mains voltage (115 or
230 V) should now be visible from the front.

Check fuses

4

Carefully take both fuses out of fuse holder 1111 and check their
specifications:

100……120 V 0.2 A (slow-blow) Metrohm-No. U.600.0009
220……240 V 0.1 A (slow-blow) Metrohm-No. U.600.0006

Insert fuses

5

Change both fuses if necessary and reinsert in fuse holder 1111.

Install fuse holder

6

Push fuse holder 1111 back into the opening of the 754 Dialysis
Unit by hand until it clicks into place properly.

8

754 Dialysis Unit

2.2.2 Fuses

2.2 Mains connection

100 – 120 V220 – 240 V

1212

1111

230

115

1111 Fuse holder
1212 Voltage selection

insert with display

1313

of voltage

1313 Mains switch

1616

1616 Mains connection

plug

Fig. 3: Setting the mains voltage

Two fuses 0.2 A/slow-blow for 100…120 V or 0.1 A/slow-blow for
220…240 V are installed in the fuse holder 1111 of the 754 Dialysis Unit
as standard.

To eliminate any danger of fire, ensure that the instrument is never
operated with any other type of fuses!

To check or change fuses, proceed as described in section 2.2.1.

2.2.3 Mains cable and mains connection

Mains cable

The instrument is supplied with one of three mains cables

• 6.2122.020 with plug SEV 12 (Switzerland, …)

• 6.2122.040 with plug CEE(7), VII (Germany, …)

• 6.2133.070 with plug NEMA 5-15 (USA, …)

which are three-cored and fitted with a plug with an earthing pin. If a
different plug has to be fitted, the yellow/green lead (IEC standard)
must be connected to protective earth (protection class I).

Any break in the earthing inside or outside the instrument can make it
a hazard!

Mains connection

Plug the mains cable into mains connection plug 1616 of the 754 Dialysis
Unit (see Fig. 3).

2.2.4 Switching the instrument on/off

The 754 Dialysis Unit is switched on and off using mains switch 1313.
When the instrument is switched on, lamp 33 lights up.

754 Dialysis Unit

9

2 Installation

2.3 Attaching the accessories

2.3.1 Electrical connection to the 732 IC Detector

In order to allow remote operation of the pump via the 732 IC Detector,
connection 1515 of the 754 Dialysis Unit must be connected to the
"Remote" connection of the 732 IC Detector by means of the

6.2143.200 cable as per Fig. 4. The switch 22 <REMOTE> on the front
of the 754 Dialysis Unit must additionally be set to "ON" to permit re­mote operation.

If you wish to connect a second, additional unit (e.g. 752, 753, 754) to
the remote interface of the 732 IC Detector, connect the Dialysis Unit
754 using the optionally available 6.2143.220 cable instead of the

6.2143.200 cable.

732

754

6.2143.200

Fig. 4: Connection of 754 Dialysis Unit to 732 IC Detector

2.3.2 Attaching the accessories to the 733.0020 IC Separation
Center

The 2.733.0020 IC Separation Center comes ready to operate with two
IC columns. To operate it with the 754 Dialysis Unit, reassemble it as
follows (see Fig. 5 and Fig. 8):

Dismantle accessories

1

• Unscrew the two sample loops mounted on valve A 1818 and

valve B 2222.

• Loosen the rotary nipples screwed onto the interior side of

feedthrough 2222 and 2828, pull the two PTFE suction tubings
right out of the connections and unscrew from connection "1"
of valve A 1818 and valve B 2222.

• Remove the PEEK capillaries running from connection "2" of

valve A 1818 and valve B 2222 to feedthroughs 2121 and 2727.

10

754 Dialysis Unit

2.3 Attaching the accessories

Connect new feedthrough

2

• Using a wrench or a spanner, unscrew the nut on the interior

side of feedthrough 2727 and remove the feedthrough.

• Insert new 6.2756.000 tubing feedthrough in the hole and

retighten the nut.

Attach tubing to valve A

3

• Screw 6.1825.210 PEEK sample loop (20 µL) to connections

"3" and "6" of valve A 1818 using the enclosed 6.2744.010 PEEK
compression fittings.

• Screw PEEK capillary 1919 (6.1831.060; L =1 m) to connection

"2" of valve A 1818 using a 6.2744.010 PEEK compression fit-
ting. Pull the other end of the tubing to the back through the
rear panel opening 4141.

• Screw PEEK capillary 2020 (6.1831.040; L =15 cm) to connec-

tion "1" of valve A 18 18 using a 6.2744.010 PEEK compression
fitting.

Attach tubing to valve B

4

• Screw the PEEK capillary 2020 (6.1831.040; L =15 cm) at-

tached to connection "1" of valve A 1818 to connection "3" of
valve B 22 22 using a 6.2744.010 PEEK compression fitting.

• Screw PEEK capillary 2121 (6.1831.040; L =15 cm) to connec-

tions "1" and "2" of valve B 2222 using two 6.2744.010 PEEK
compression fittings.

• Screw PEEK capillary 2525 (6.1831.050; L =40 cm) to connec-

tion "4" of valve B 2222 using a 6.2744.010 PEEK compression
fitting and pull the other end to the front through feedthrough

2727.

• Screw PEEK capillary 2424 (6.1831.050; L =40 cm) to connec-

tion "5" of valve B 2222 using a 6.2744.010 PEEK compression
fitting and pull the other end to the front through feedthrough

2727.

• Screw PEEK capillary 2323 (6.1831.050; L =40 cm) to connec-

tion "6" of valve B 2222 using a 6.2744.010 PEEK compression
fitting and pull the other end to the front through feedthrough

2828.

Connecting to the 753 Suppressor Module (Option)

5

• Insert the suppressor block in the 733 IC Separation Center

as per the 753 Instructions for Use.

• Connect tubing to the IC column, the detector block and the

waste in the 733 IC Separation Center as per the 753 Instruc­tions for Use.

• Pull the two inlet capillaries for H2SO4 and H2O to the front

through feedthrough 2727 and connect to the 753 Suppressor
Module pump tubings.

754 Dialysis Unit

11

2 Installation

1919

1818

20

21

22

23

24

1717

FILL INJECT

A B

STEP

FILL INJECT

4141

Fig. 5: Interior of the 733.0020 IC Separation Center

1717 PEEK sample loop

(6.1825.210; 20 µµL )

1818 Injection valve A 2525 PEEK capillary

1919 PEEK capillary

(6.1831.060; 1 m)

2424 PEEK capillary

(6.1831.050; 40 cm)

(6.1831.050; 40 cm)

2121 Connection for syringe

2727

25

28282121 2222

12

2020 PEEK capillary

2222 Feedthrough

(6.1831.040; 15 cm)

2121 PEEK capillary

2727 Feedthrough

(6.1831.040; 15 cm)

2222 Injection valve B 2828 Feedthrough
2323 PEEK capillary

4141 Rear panel opening

(6.1831.040; 15 cm)

754 Dialysis Unit

2.3 Attaching the accessories

2.3.3 Attaching the accessories to the 754 Dialysis Unit

Attach the tubing cartridges with the pump tubings to the 754 Dialysis
Unit. Proceed as follows (see Fig. 5 and Fig. 8):

Attach pump tubings

9

• Loosen both tubing cartridges 44 from the holding clamp 77 by

pressing down snap-action lever 88 and remove from mount-
ing pin 1010 on 754 Dialysis Unit (see Fig. 1).

• Press contact pressure lever 55 on both tubing cartridges

down as far as it will go.

• Insert a length of pump tubing 3131 (6.1826.030) for feeding the

acceptor solution into the first tubing cartridge as shown in
Fig. 6. The orange-yellow stopper 3232 must click into the cor-
responding holder on the left-hand side of the tubing car­tridge.

• Insert a length of pump tubing 3030 (6.1826.040) for feeding the

sample into the second tubing cartridge. The black-black
stopper 3232 must click into the corresponding holder on the
left-hand side of the tubing cartridge.

• Place the tubing cartridge for the acceptor solution and then

the tubing cartridge for the sample on mounting pin 1010 and
press down on the right-hand side until snap-action lever 88
clicks into position on holding clamp 77. Take care that no
kinks are formed in the pump tubing.

Tubing connections for acceptor solution

2

• Mount a PEEK compression fitting 2828 (6.2744.010) on the

end of the PEEK capillary 23 23 projecting from feedthrough 2828
(see Fig. 5) and screw it on to coupling 2929 (6.2744.030) (see
Fig. 6).

• Push coupling 2929 on to the outlet end of the pump tubing 3131
(see Fig. 6 and Fig. 8).

• Cut a piece of PTFE tubing (6.1803.030) to the desired length
for the suction tubing 2626.

• Mount a PEEK compression fitting 2828 (6.2744.010) on one
end of suction tubing 2626 and screw this on to coupling 2929
(see Fig. 6).

• Push coupling 2929 on to the inlet end of the pump tubing 3131
(see Fig. 6 and Fig. 8).

• Immerse the other end of the suction tubing 2626 in a vessel
containing acceptor solution and fix in place. The acceptor
solution is usually ultrapure water that has previously been
degassed (with N2, He or a vacuum).

754 Dialysis Unit

13

2 Installation

26/2726/27

2929 30/3130/31 3232 44 55 3232 2929 23/3323/3388 28282828

Fig. 6: Installing pump tubings

44 Tubing cartridge 2828 PEEK compression fitting

(6.2744.010)

55 Contact pressure lever 2929 Coupling (6.2744.030)
88 Snap-action lever 3030 Pump tubing (6.1826.040)

for conveying the sample

2323 PEEK capillary (6.1831.050)

for conveying the acceptor
solution to valve B

2626 PTFE capillary (6.1803.030)

for feeding the sample in

2727 PTFE capillary (6.1803.030)

for feeding the acceptor solution
in

Tubing connections for sample

3

• Mount a PEEK compression fitting 2828 (6.2744.010) on the
end of PTFE capillary tubing 3333 (6.1803.050, L = 20 cm) and
screw this on to coupling 2929 (6.2744.030) (see Fig. 6).

• Push coupling 2929 on to the outlet end of the pump tubing 3030
(see Fig. 6 and Fig. 8).

• Cut a piece of PTFE tubing (6.1803.030) to the desired length
for the suction tubing 2727.

• Mount a PEEK compression fitting 2828 (6.2744.010) on one
end of suction tubing 2727 and screw this on to coupling 2929
(see Fig. 6).

• Push coupling 2929 on to the inlet end of the pump tubing 3030
(see Fig. 6 and Fig. 8).

• Immerse the other end of suction tubing 2727 in the sample
vessel and fix in place.

3131 Pump tubing (6.1826.030)

for conveying the acceptor solution

3232 Stopper on pump tubing

3333 PTFE capillary (6.1803.050)

for conveying sample to dialysis cell

14

754 Dialysis Unit

Pump tubings are consumable material with a lifetime which depends
on the contact pressure. This is why the tubing cartridges should be
raised completely by loosening snap-action lever 88 on the right-hand
side if the pump is to remain switched off for a considerable length of
time (the set contact pressure remains unchanged).

The 6.1826.0X0 pump tubing is made of PVC and must not be used
for rinsing with solutions which contain acetone. In such cases, rinse
with different pump tubing or a different pump.

2.3.4 Assembling the dialysis cell

A dialysis membrane has to be inserted before the dialysis cell can be
placed inside the 754 Dialysis Unit. Proceed as follows (see Fig. 7):

Prepare dialysis cell

1

• Extract dialysis cell 3434 (6.2729.100) from its packaging and

remove the four 6.2744.060 dummy stoppers.

• Using the 6.2621.070 Allen key, completely loosen the 5

screws 3939, separate top part 3838 from bottom part 3535 and re-
move sealing ring 3636.

• Thoroughly rinse the sealing ring 3636, bottom part 3535 and top

part 3838 of the dialysis cell with ultrapure water and dry with N
or a lint-free cloth.

2.3 Attaching the accessories

2

Use only ultrapure water or ethanol to clean the dialysis cell;
organic solvents (e.g. acetone) will cause damage to the plexiglas
cell !

Prepare dialysis membrane

2

• Use the 6.2831.010 pincette to extract a new dialysis mem-

brane 3838 (e.g. 6.2714.010) from its packaging and immerse
in a Petri dish containing ultrapure water for approx. 2 min
until the membrane is completely saturated with water.

Insert dialysis membrane

3

• Lay bottom part 3535 on a paper wipe with its inside facing

upwards.

• Insert sealing ring 3636 in the appropriate recess in bottom part

3535.

• Using the 6.2831.010 pincette, place the wet dialysis mem-

brane 3838 (e.g. 6.2714.010) inside the sealing ring 3636 on the
bottom part 3535.

Once saturated, take care that the dialysis membrane is not allowed to
dry out, otherwise it cannot be used any more !

754 Dialysis Unit

15

2 Installation

Close dialysis cell

4

• Place top part 3838 on bottom part 3535 so that both parts lie
flush.

• Using the 6.2621.070 Allen key, screw the 5 screws 39 39 right
in and tighten up.

Attach dialysis cell

5

• Insert the assembled dialysis cell 3434 in the cell holder 66 of the
754 Dialysis Unit.

3434 3535 3636 3838 39393737

16

4040

4141 4242 4343 4444 4040

Fig. 7: Assembling the dialysis cell

3434 Dialysis cell (6.2729.100) 4040 Slot for positioning the dialysis

cell in cell holder 66

3535 Bottom part of the dialysis

cell

3636 Sealing ring (E.301.0111) 4242 Inlet for acceptor solution
3737 Dialysis membrane

(e.g. 6.2714.010)

3838 Top part of the dialysis cell 4444 Outlet for sample solution
3939 Screws V.022.6030 incl.

washer 4.754.4090

4141 Outlet for acceptor solution

4343 Inlet for sample solution

754 Dialysis Unit

2.3.5 Connecting the dialysis cell

To connect the dialysis cell to the IC system proceed as follows (see
Fig. 8):

Tubing connections for acceptor solution

1

• Mount a PVDF compression fitting 5050 (6.2744.000) on the

end of PEEK capillary 24 24 projecting from feedthrough 2727 (see
Fig. 5).

• Screw PEEK capillary 2424 to inlet opening 4242 of the bottom
part 3535 of the cell (see Fig. 7).

• Mount a PVDF compression fitting 5050 (6.2744.000) to the end
of PEEK capillary 25 25 projecting from feedthrough 2727 (see Fig.

5).

• Screw PEEK capillary 2525 to outlet opening 4141 of the bottom
part 3535 of the cell (see Fig. 7).

Tubing connections for sample

2

• Mount a PVDF compression fitting 5050 (6.2744.000) on the
end of the PTFE capillary 33 33 connected to pump tubing 3030.

• Screw PTFE capillary 3333 to inlet opening 4343 of the top part 3838
of the cell (see Fig. 7).

• Mount a PVDF compression fitting 5050 (6.2744.000) to one
end of the PTFE capillary 4545 (6.1803.040; L = 1 m).

• Screw PTFE capillary 4545 to outlet opening 4444 of the top part
3838 of the cell (see Fig. 7).

2.3 Attaching the accessories

Do not connect the dialysis cell with anything other than the

6.2744.000 PVDF compression fittings supplied. If 6.2744.010
PEEK compression fittings are used, stress cracks may appear in the
dialysis cell !

754 Dialysis Unit

17

2 Installation

27 26

20

17

733

A B

Column

Eluent

754

21

23
24

25

19

45
46

47

48

49

Acceptor Waste

Sample

1717 PEEK sample loop

(6.1825.210; 20 µµL)

1919 PEEK capillary (6.1831.060; 1 m)

Connection Valve A – Waste

2020 PEEK capillary (6.1831.040; 15 cm)

Connection Valve A – Valve B

2121 PEEK capillary (6.1831.040; 15 cm)

Connection at Valve B

2323 PEEK capillary (6.1831.050; 40 cm)

Connection Pump tubing – Valve B

2424 PEEK capillary (6.1831.050; 40 cm)

Connection Valve B – Dialysis cell

30 28 33 34

Fig. 8: Diagram of tubing connections

50 502931

3030 Pump tubing (6.1826.040)

for conveying the sample

3131 Pump tubing (6.1826.030)

for conveying the acceptor solution

3333 PTFE capillary (6.1803.050; 20 cm)

Connection Pump tubing – Dialysis cell

3434 Dialysis cell (6.2729.100)

4545 PTFE capillary (6.1803.040; 1 m)

Connection Dialysis cell – Waste

4646 PVDF compression fitting

(6.2744.000)

2525 PEEK capillary (6.1831.050; 40 cm)

Connection Valve B – Dialysis cell

2626 PTFE capillary (6.1803.030)

for feeding the sample in

2727 PTFE capillary (6.1803.030)

for feeding in the acceptor solution in

2828 PEEK compr. fitting (6.2744.010) 5050 PVDF compr. fitting (6.2744.000)
2929 Coupling (6.2744.030)

4747 PEEK compression fitting

(6.2744.010)

4848 Bottle attachment (6.1602.150)

4949 Waste bottle (6.1608.070)

18

754 Dialysis Unit

2.3 Attaching the accessories

2.3.6 Attaching the accessories to the waste bottle

The waste bottle which receives the sample and acceptor solutions is
connected to the IC system as follows (see Fig. 8):

Attach bottle attachment

1

• Screw bottle attachment 4848 (6.1602.150) on to waste bottle
4949 (6.1608.070).

Connect tubing to the dialysis cell

2

• Pull one end of the PTFE capillary 45 45 connected to the dialy-
sis cell through a PVDF compression fitting 4646 (6.2744.000),
allowing 5 cm of tubing to project.

• Screw the PVDF compression fitting 4646 with tubing into an
opening in the bottle attachment 4848 and tighten so that the
tubing is held secure.

Connect tubing to valve A

3

• Pull the free end of the PEEK capillary 19 19 screwed to connec-
tion "2" of valve A through a PEEK compression fitting 4747
(6.2744.010), allowing 5 cm of tubing to project.

• Screw the PEEK compression fitting 4747 with tubing into an
opening in the bottle attachment 4848 and tighten so that the
tubing is held secure.

The end of the two tubings 1919 and 4545 inserted in the waste bottle
must be at the same height !

754 Dialysis Unit

19

2 Installation

2.3.7 Conditioning the dialysis system

Before effecting the first analysis, rinse the dialysis cell, the inserted di­alysis membrane and all tubing connections with ultrapure water. Pro­ceed as follows:

Settings on the 733 IC Separation Center

1

• Press the <FILL> key for injection valve A on the 733 IC

Separation Center. This switches injection valve A to the
"FILL" position.

• Press the <FILL> key for injection valve B on the 733 IC

Separation Center. This switches injection valve B to the
"FILL" position.

Starting up the 754 Dialysis Unit

2

• Immerse the two suction tubings 2626 and 2727 in the acceptor

solution (ultrapure degassed water).

• Set the <REMOTE> switch 22 on the 754 Dialysis Unit to

"OFF".

• Switch on the 754 Dialysis Unit with mains switch 1313 and set

the <PUMP> switch 33 to "ON".

• Adjust the contact pressure for both tubing cartridges: Press

contact pressure lever 55 upwards until the solutions just start
to be drawn in. Then push the contact pressure lever up­wards until it clicks again to achieve the optimum contact
pressure.

• Rinse the dialysis system with ultrapure water for approx.

10 min and check that the solutions flow steadily through the
two tubings into the waste bottle.

20

Conditioning the dialysis membrane

3

• Press the <INJECT> key for injection valve B on the 733 IC

Separation Center. This switches injection valve B to the
"INJECT" position.

• Rinse the dialysis system with ultrapure water for approx.

20 min. Check that the solutions flow steadily through the two
tubings into the waste bottle.

• Check all tubing from the storage vessels through the tubing

cartridges and the dialysis cell up to the waste containers for
any leaks. If liquid escapes anywhere, tighten or replace the
corresponding connection.

• If air bubbles remain in the dialysis cell, unscrew PEEK tubing

2525 (Acceptor solution) and PTFE tubing 4545 (sample) from
outlets 4141 and 4444 of the dialysis cell and wait until the air
bubbles disappear. Reconnect the tubings to the dialysis cell.

754 Dialysis Unit

3 Operation

The 754 Dialysis Unit cannot be manually operated unless the installa­tion has been carried out properly as described in section 2 (mains
connection, attaching pump tubing and dialysis cell, connection to
the IC system).

3.1 Manual operation

Switch instrument on/off

The 754 Dialysis Unit is switched on and off using mains switch
1313 on the rear of the instrument (see Fig. 2):

I Instrument switched on

0 Instrument switched off

3.1 Manual operation

REMOTE

ON

OFF

PUMP

ON

OFF

POWER

After the instrument has been switched on the power lamp 33
lights up to show that the instrument is ready for use.

Switch off remote control of the pump

In order to allow manual operation the remote control of the pump
must be switched off with switch 22.

ON Remote control switched on

OFF Remote control switched off

Switch pump on/off

The drive of the pump is switched on and off with the switch 11
<PUMP>:

ON Pump switched on
OFF Pump switched off

754 Dialysis Unit

21

3 Operation

3.2 Operation via 732 IC Detector

Switch instrument on/off

The 754 Dialysis Unit is switched on and off using mains switch
1313 on the rear of the instrument (see Fig. 2):

I Instrument switched on

0 Instrument switched off

POWER

REMOTE

ON

OFF

Switch on:

After the instrument has been switched on the power lamp 33
lights up to show that the instrument is ready for use.

Switch on remote control of the pump

In order to allow remote operation via 732 IC Detector, the remote
control must be switched on with switch 22.

ON Remote control switched on

OFF Remote control switched off

Switch pump on/off in a program

In the 732 IC Detector the <PROGRAM> key can be used to
create time programs with a maximum of 20 program steps (see
section 4.7.1 of 732/733 Manual). The pump can be switched on
or off at any program step by setting the remote output lead (lead
1 or 4 depending on the cable) to 1 (on, active, 0 V) or 0 (off,
inactive, open) respectively:

with 6.2143.200 cable with 6.2143.220 cable

>PROGRAM/edit XXX.X min
remote 1TTTTTTT

>PROGRAM/edit XXX.X min
remote TTT1TTTT

22

Switch off:

Switch on:

Switch off:

>PROGRAM/edit XXX.X min
remote 0TTTTTTT

>PROGRAM/edit XXX.X min
remote TTT0TTTT

Switch pump on/off at an event

In the 732 IC Detector the <EVENT> key can be used to
program a maximum of 4 different events (see section 4.7.3 of
732/733 Manual). The pump can be switched on or off at any
event by setting the remote output lead (lead 1 or 4 depending on
the cable) to 1 (on, active, 0 V) or 0 (off, inactive, open) respec­tively:

with 6.2143.200 cable with 6.2143.220 cable

EVENT YY-MM-DD HH:MM:SS
remote 1TTTTTTT

EVENT YY-MM-DD HH:MM:SS
remote 0TTTTTTT

EVENT YY-MM-DD HH:MM:SS
remote TTT1TTTT

EVENT YY-MM-DD HH:MM:SS
remote TTT0TTTT

754 Dialysis Unit

3.3 The dialysis procedure

3.3.1 Dialysis flow chart

Every sample dialysed with the 754 Dialysis Unit comprises the follow­ing four steps:

Rinsing

1

Valve A: FILL
Valve B: INJECT

Sample channel, ac­ceptor channel and
sample loop are rinsed
(rinsing time: approx. 2
min).

Dialysis with «Stopped Flow»

2

Valve A: FILL
Valve B: FILL

The sample is dialysed
with resting acceptor
solution (dialysis time:
approx. 10 min).

3.3 The dialysis procedure

Fill Inject

A B

Column

Eluent

Acceptor

Sample

Fill Fill

A B

Column

Eluent

Acceptor

Sample

Waste

Waste

Transfer to sample loop

3

Valve A: FILL
Valve B: INJECT

The acceptor solution
enriched with ions from
the sample is trans­ferred to the sample
loop (transfer time:
approx. 0.4 to 0.6 min).

Injection

4

Valve A: INJECT
Valve B: INJECT

The acceptor solution
enriched with ions from
the sample is injected
into the IC system.

Fill Inject

A B

Column

Eluent

Acceptor

Sample

Inject Inject

A B

Column

Eluent

Acceptor

Sample

Waste

Waste

754 Dialysis Unit

23

3 Operation

3.3.2 Programming with the 732 IC Detector

Program without suppression

To dialyse in an IC system without suppression a program has to be
created for controlling the valves in the 733 IC Separation Center and
the 754 Dialysis Unit connected with cable 6.2143.200. The following
example program contains the necessary program steps.

PROGRAM
progr.type: cycle

number of cycles 1

Rinsing time

Dialysis time

Transfer time

Programming

time

0.0 valve A: fill
valve B: inject
remote: 1*******

2.0 valve B: fill

12.0 zero: on
valve B: inject

12.5 valve A: inject
remote 0*******

15.0 flag: end

Rinsing of sample loop
Rinsing of dialysis cell
Start of 754 Pump

Stop of acceptor solution
Trigger the auto-zero function

Transfer to sample loop
Injection (Õ Start evaluation)

Stop of 754 Pump
End of program

Rinsing time

Dialysis time

Transfer time

Programming

time

Program with suppression

To dialyze in an IC system with suppression a program has to be cre­ated for controlling the valves in the 733 IC Separation Center and the
754 Dialysis Unit and 753 Suppressor Module connected with cable

6.2143.220. The following example program contains the necessary
program steps.

PROGRAM
progr.type: cycle

number of cycle 1

0.0 valve A: fill
valve B: inject
remote: *0*1****

2.0 valve B: fill

12.0 zero: on
valve B: inject
remote: *1******

12.5 valve A: inject
remote ***0****

15.0 flag: end

Rinsing of sample loop
Rinsing of dialysis cell
Resetting of the remote line for the
suppressor and start of 754 Pump

Stop of acceptor solution
Trigger the auto-zero function

Transfer to sample loop
Switch suppressor to next position

Injection (Õ Start evaluation)
Stop of 754 Pump

Program end

24

754 Dialysis Unit

3.3.3 Setting the rinsing time

A rinsing time of 2 min is normally sufficient to ensure thorough rinsing
of the sample and acceptor channels. This time can be increased if re­quired.

3.3.4 Setting the transfer time

The time allowed for transferring the enriched acceptor solution to the
sample loop must be set to ensure that the part of the acceptor solution
with the highest concentration of ions is transferred to the sample loop.
With standard accessories the optimum transfer time depends on the
rate of operation of the pump; the following values can be used as a
guideline:

Mains frequency Optimum transfer time

50 Hz 0.5 min
60 Hz 0.4 min

3.3 The dialysis procedure

For each analysis problem, determine the optimum transfer time by
taking measurements of the individual ion concentrations in relation to
the transfer time itself and verify your measurements from time to time.
Proceed as follows:

First measurement

1

• Set the transfer time in the program to 0.2 min and the dialy­sis time to 10 min (see section 3.3.2).

• Immerse sample suction tubing 2626 in the standard with 10
mg/L of the required anion or cation.

• Start the program on the 732 IC Detector with the <PROG
R/S> key. Wait until the LED in the <PROG R/S> key stops
flashing, signalling the end of the measuring process.

Additional measurements

2

• Increase the transfer time in the program (see section 3.3.2)
by increments of 0.1 min until the concentration measured
falls again.

• Take measurements as in point 1.

Determining the optimum transfer time

3

• Record the measured peak area resp. concentration in rela­tion to the transfer time and determine the optimum transfer
time.

754 Dialysis Unit

The Figure below shows an example of this determination process for
anions using the 6.2714.010 dialysis membrane (cellulose acetate;
thickness = 115 µm; pore size = 0.2 µm) available from Metrohm. The
sample was a standard solution containing 10 mg/L of each of the indi­vidual anions, which was dialysed for 10 min (mains frequency 50 Hz).

25

3 Operation

12.0

10.0

8.0

6.0

4.0

Concentration [mg/L]

2.0

0.0

0.0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0

3.3.5 Setting the dialysis time

After calculating the optimum transfer time, determine the optimum di­alysis time. This value will depend on the total ion concentration. The
dialysis time with a stopped acceptor flow should be selected so that
100% of the sample concentration is achieved in the acceptor solution.
For the 6.2714.010 dialysis membrane (cellulose acetate; thick­ness = 115 µm; pore size = 0.2 µm) available from Metrohm, the opti­mum dialysis time for a total ion concentration of ≥ 5 mg/L is 10 min.

Fluoride
Chloride
Nitrite
Bromide
Nitrate
Phosphate
Sulphate

Transfer time [min]

If a different dialysis membrane is used, or measurements are taken
with a total ion concentration of < 5 mg/L, then the optimum dialysis
time will have to be redetermined by measuring the recovery rate in re­lation to time. Proceed as follows:

Measure the standard directly

1

• Switch off remote control of the 754 Dialysis Unit with the
<REMOTE> key 22 (see section 3.1).

• Set the two valves A and B on the 733 IC Separation Center
to "FILL" with keys 2323 and 2525.

• Immerse suction tubing 2727 in the required ion standard,
which has approximately the same total ion concentration as
the samples.

• Switch on the pump on the 754 Dialysis Unit with the
<PUMP> key 11.

• After approx. 2 min, switch valve A on the 733 IC Separation
Center to "INJECT" with key 2424 and simultaneously start the
data evaluation system. In operation with the 753 Suppressor
Module, the suppressor has to be rotated with the <STEP>
key.

• Immerse suction tubing 2727 in the acceptor solution and rinse
the acceptor channel for approx. 2 min.

• Switch off the pump on the 754 Dialysis Unit with the
<PUMP> key 11.

• Switch the remote control of the 754 Dialysis Unit back on
with the <REMOTE> key 22.

26

754 Dialysis Unit

3.3 The dialysis procedure

Measure the standard with dialysis

2

• Set the dialysis time in the program to 5 min (see section

3.3.2).

• Immerse sample suction tubing 2626 in the standard used in

point 1.

• Start the program on the 732 IC Detector with the <PROG

R/S> key. Wait until the LED in the <PROG R/S> key stops
flashing, signalling the end of the evaluation.

Additional measurements

3

• Increase the dialysis time in the program (see section 3.3.2)

by 5 min increments until the measured values remain con­stant.

• Take measurements as per point 2.

Determine optimum dialysis time

4

• Record the ratio of the peak surfaces, i.e. concentrations

measured with and without dialysis in relation to the dialysis
time (i.e. the recovery rate) and determine the optimum dialy­sis time.

3.3.6 Setting the program time

Set the program time so that the chromatogram can be completely re­corded and evaluated within this period.

3.3.7 Dialysis procedure

To analyse samples using dialysis you should preferably proceed as
follows:

Prepare acceptor solution

1

• To avoid malfunctions caused by air bubbles in the acceptor

channel, the ultrapure water used for the acceptor solution
should always be degassed for at least 10 min with vacuum,
N2 or He.

Prepare sample

2

• To prevent blockages in the sample channel, samples con-

taining a high volume of (suspended/solid particles) should
always be centrifuged using a table centrifuge at 10,000
rev./min for 5 min.

754 Dialysis Unit

27

3 Operation

Starting up the IC System

3

• Switch on the 732 IC Detector, load method and set program
status to active (see the 732/733 Manual).

• Switch on 709 IC Pump and condition IC column (see the 709
Manual).

• If applicable, switch on the 753 Suppressor Module and the
remote control (see the 753 Manual).

• Switch on data evaluation system (e.g. «IC Metrodata for
Win95»).

Starting up the dialysis system

4

• Switch on the 754 Dialysis Unit and switch off the remote
control with the <REMOTE> key 22 (see section 3.1).

• Immerse suction tubings 2626 and 2727 in the acceptor solution,
switch on the pump with the <PUMP> key 1 1 and rinse the
dialysis system with acceptor solution for approx. 10 min.

• Switch off the pump with the <PUMP> key 11 and switch on
the remote control with the <REMOTE> key 22.

Calibration

5

• Immerse sample suction tubing 2626 in the standard solution.

• Start program on the 732 IC Detector with the <PROG R/S>

key. The LED in the key will start to flash.

• The next standard can be measured as soon as the LED in
the <PROG R/S> key stops flashing.

Measuring the sample

6

• Immerse sample suction tubing 2626 in the sample solution.

• Start program on the 732 IC Detector with the <PROG R/S>

key. The LED in the key will start to flash.

• The next sample can be measured as soon as the LED in the
<PROG R/S> key stops flashing.

Rinse the dialysis system

7

• After completing the measurements, switch off the remote
control on the 754 Dialysis Unit with the <REMOTE> key 22
(see section 3.1).

• Immerse sample suction tubing 2626 in the acceptor solution,
switch on the pump with the <PUMP> key 1 1 and rinse the
dialysis system with acceptor solution for approx. 10 min.

• Switch off the pump with the <PUMP> key 11.

28

Shutting down the dialysis system

8

• If the dialysis cell is to remain switched off for a long period of
time, remove the inlet and outlet leads connecting to the di­alysis cell and block openings 4141, 4242, 4343 and 4444 (see Fig. 7)
with 6.2744.060 dummy stoppers.

754 Dialysis Unit

4.1 Malfunctions and their rectification

4 Malfunctions – Maintenance

4.1 Malfunctions and their rectification

If difficulties appear with the IC system during analysis, their cause are
best investigated in the order separating column →→ pump →→ eluent
→→ IC system. An overview of possible malfunctions, their causes and
remedies can be found in the instruction manuals of the 732/733 IC
System (section 5.3.2). In addition to these general malfunctions the
table below contains others which might arise as a result of the opera­tion of the 754 Dialysis Unit.

Malfunction Possible causes Rectification

Insufficient or non­existing flow rate at
754 Dialysis Unit

Leakage • Leaking compression fittings • Tighten the compression fittings or replace

Air bubbles in pump
circuit

• Contact pressure too low

• Pump tubing faulty

• Inlets to dialysis cell blocked

• Sample tubing blocked by

particles that are too large

• Bacterial growth in the sample

channel and dialysis cell

• Overly narrow PTFE tubings due

to excessive tightening of the
compression fittings

• Suction tubing is not immersed

• Leaking compression fittings

• Acceptor solution insufficiently

degassed

• Standing air bubbles

• Set contact pressure correctly with contact

pressure lever (see section 2.3.3).

• Replace pump tubing
(see section 4.2.5).

• Replace the dialysis membrane and clean
the cell (see section 4.2.6).

• Replace blocked tubing; always centrifuge
samples (see section 3.3.7).

• Periodically replace tubings 2626, 3030, 3333 and
4545 for the sample channel; replace the

dialysis membrane

• Replace tubings.

if necessary.

• Ensure suction tubing is fully immersed in
the acceptor or sample solution.

• Tighten the compression fittings or replace
if necessary.

• Re-degas the acceptor solution.

• Small air bubbles are not a problem. If big

air bubbles occur, rinse the sample chan­nel with degassed acceptor solution (open
outlet briefly if need be).

Insufficient yield from
dialysis

754 Dialysis Unit

• Transfer time incorrectly set

• Dialysis time too short (e.g.

when concentration is too low)

• Dried out or damaged dialysis

membrane

• Deposits on the dialysis mem-

brane, recognizable from dis­colouring at the sample inlet

• Unsuitable dialysis membrane

• Check optimum transfer time, especially

after each change of membrane or tubing
(see section 3.3.4).

• Check optimum dialysis time (see section

3.3.5).

• Replace dialysis membrane
(see section 4.2.6).

• Replace dialysis membrane
(see section 4.2.6).

• Use different dialysis membrane.

29

4 Malfunctions – Maintenance

4.2 Maintenance and servicing

4.2.1 Handling dialysis membranes

Dialysis membranes are very sensitive to contact and drying out. Avoid
touching them by hand and ensure that the membrane in the cell never
dries out.

The service life of a dialysis membrane is approx. 1 week under fre­quent use. Do not hesitate to replace the membrane if signs of de­creased yield or blockages become apparent.

Please ensure you follow the instructions on how to install the dialysis
cell (section 2.3.4), how to shut it down (section 4.2.4) and how to re-
place the dialysis membrane (section 4.2.6).

4.2.2 Care of the unit

The 754 Dialysis Unit requires proper care and attention. Excessive
contamination of the instrument could possibly lead to malfunctions
and a shorter service life of the inherently rugged mechanical and elec­tronic parts.

Wipe up spilled chemicals and solvents immediately. It is especially
important to protect the plug connections at the rear of the instrument
(particularly the mains plug) against contamination.

The unit has been constructed in such a way as to virtually eliminate
the possibility of penetration of corrosive media into the interior of the
instruments. If such a situation does occur, disconnect the mains
plug of the 754 Dialysis Unit immediately to prevent extensive damage
to the instrument electronics. Inform Metrohm service if your instru­ment has been damaged in such a way.

The instrument must not be opened by untrained personnel. Please
comply with the safety notes in section 1.5.1.

4.2.3 Maintenance by Metrohm service

Maintenance of the 754 Dialysis Unit is best done as part of an annual
service performed by specialists from the Metrohm company. If work is
frequently performed with caustic and corrosive chemicals, it may be
necessary to shorten the interval between servicing.

The Metrohm service department is always willing to offer expert advice
on the maintenance and servicing of all Metrohm instruments.

30

754 Dialysis Unit

4.2.4 Shutdown

If the dialysis cell is to be switched off a long period of time the accep­tor and sample channels should be rinsed with ultrapure water for ap­prox. 10 min. Then remove the inlet and outlet leads from the dialysis
cell and block each of the openings 4141, 4242, 4343 and 4444 (see Fig. 7) with
a 6.2744.060 dummy stopper.

4.2.5 Replacing the pump tubing

Pump tubings are consumable material with a limited lifetime and
should be replaced at regular intervals (approx. every 2 weeks under
continuous use).

The working life of pump tubing depends to a considerable extent on
the contact pressure. This is why the contact pressure must be cor­rectly set as described in section 2.3.3. If the pump is to remain
switched off for a lengthy period of time the tubing cartridges should be
raised completely by loosening snap-action lever 88 on the right-hand
side (the pre-set contact pressure remains unchanged).

4.2 Maintenance and servicing

To replace a pump tubing proceed as follows:

Remove old pump tubing

1

• Press contact pressure lever 55 on the tubing cartridge down

as far as it will go.

• Release tubing cartridge 44 from holding clamp 77 by pressing

down snap-action lever 88 and remove from mounting pin 1010
on the 754 Dialysis Unit (see Fig. 1).

• Remove old pump tubing.

Insert new pump tubing

2

• Insert the new pump tubing 3030 (6.1826.040) or 3131
(6.1826.040) in the tubing cartridge as shown in Fig. 6. The
stopper 3232 must click into the corresponding holder on the
left-hand side of the tubing cartridge.

• Place the tubing cartridge on mounting pin 1010 and press
down on the right-hand side until snap-action lever 88 clicks
into position on holding clamp 77. Take care that no kinks are
formed in the pump tubing.

754 Dialysis Unit

Set contact pressure

3

• Press contact pressure lever 55 upwards until the solution just
starts to be drawn in. Then press contact pressure lever up­wards until it clicks once more to obtain optimal contact pres­sure.

31

4 Malfunctions – Maintenance

4.2.6 Replacing the dialysis membrane

The dialysis membrane may have to be replaced in the following cases
(see section 4.1 as well):

• Reduced yield from dialysis

• Membrane has dried out, or is damaged by depos-

its or bacterial growth

• Irremediable blockage of the sample channel (sample

can no longer be conveyed through the dialysis cell)

To replace the membrane, proceed as follows (see Fig. 7):

Dismantle the dialysis cell

1

• Unscrew the four inlet/outlet leads from the dialysis cell 3434

and remove cell from cell holder 66.

• Loosen the 5 screws 39 39 completely using the 6.2621.070

Allen key and separate the top part 3838 from the bottom part

3535.

• Remove old dialysis membrane 3737.

Use only ultrapure water or ethanol to clean the dialysis cell;
organic solvents (e.g. acetone) will cause damage to the plexiglas
cell !

Clean dialysis cell

2

• Rinse the sealing ring 3636, the bottom part 3535 and the top part

3838 of the dialysis cell thoroughly with ultrapure water and dry

with N2 or a lint-free cloth.

Prepare dialysis membrane

3

• Take a new dialysis membrane 3838 (e.g. 6.2714.010) out of its

packaging using the 6.2831.010 pincette and immerse in a
Petri dish containing ultrapure water for approx. 2 min until
the membrane is completely saturated with water.

Insert dialysis membrane

4

• Place bottom part 3535 on a paper wipe with the inside facing

upwards.

• Insert sealing ring 3636 in the appropriate recess in the bottom

part 3535.

• Using the 6.2831.010 pincette, insert the wet dialysis mem-

brane 3838 (e.g. 6.2714.010) inside the sealing ring 3636 on the
bottom part 3535.

32

754 Dialysis Unit

4.2 Maintenance and servicing

Close dialysis cell

5

• Place top part 3838 on bottom part 3535 so that both parts lie
flush.

• Screw the 5 screws 3939 in all the way in and tighten using the

6.2621.070 Allen key.

Attach dialysis cell

6

• Place the assembled dialysis cell 3434 in cell holder 66 of the
754 Dialysis Unit.

Connect dialysis cell

7

• Screw PEEK capillary 2424 to inlet opening 4242 of the bottom
part 3535 of the cell.

• Screw PEEK capillary 2525 to outlet opening 4141 of the bottom
part 3535 of the cell.

• Screw PTFE capillary 3333 to inlet opening 4343 of the top part 3838
of the cell.

• Screw PTFE capillary 4545 to the outlet opening 4444 of the top
part 3838 of the cell.

Condition dialysis membrane

8

• Immerse suction tubings 2626 and 2727 in the acceptor solution,
switch on pump with the <PUMP> key 1 1 and rinse the di-
alysis system with acceptor solution for approx. 20 min.
Check that the solution flows steadily from the two lines into
the waste bottle.

• Check all tubings between the storage vessels, the tubing
cartridges, the dialysis cell and the waste bottles for leaks. If a
leak occurs anywhere, tighten or replace the connection in
question.

• Switch off pump with the <PUMP> key 11.

754 Dialysis Unit

33

4 Malfunctions – Maintenance

34

754 Dialysis Unit

5 Appendix

external

754

5.1 Technical data

Dialysis cell

Material Plexiglass (polymethyl methacrylate)
Solvent compatibility Water, ethanol

Cell volume 240 µL (from inlet to outlet opening)
Diameter of membrane 47 mm

Pump

Pump type 2-channel peristaltic pump with rotational speed

5.1 Technical data

(no organic sovents!)

of

20/min (50 Hz)
24/min (60 Hz)

Pump capacity
(with water,
without counterpressure)

with 6.1826.030 pump tubing:

typically 0.5…0.6 mL/min

with 6.1826.040 pump tubing:

typically 0.9…1.1 mL/min

Pressure max. 1.5 bar (0.15 MPa)
Pump tubing material PVC (Tygon)

Mains connection

Mains voltage 115 V: 100...120 V ± 10 %

230 V: 220...240 V ± 10 %
Switchable with voltage selector in fuse holder
(see section 2.2.1)

Mains frequency 50...60 Hz
Power consumption 12 VA
Fuse 5 mm Ø, 20 mm length

100…120 V: 0.2 A (slow-blow)
220…240 V: 0.1 A (slow-blow)

Remote interface

Purpose

Remote control of pump (switching on/off)

754 Dialysis Unit

Pin assignment

+5V

330

Pin

Current flows: on
Current does not flow: off

2

+

-

5

35

5 Appendix

Safety specifications

Construction / Testing According to IEC 1010 / EN 61010 / UL 3101-1,

protection class I, degree of protection IP40

Safety directions The Instructions for Use include information and

warnings to which the user must pay attention in
order to assure safe operation of the instrument.

Electromagnetic compatibility (EMC)

Emitted interference Standards met:

EN55011 (class B), EN55022 (class B),
EN50081-1

Immunity to interference Standards met:

IEC801-2/IEC1000-4-2 (class 4), IEC801-3/
IEC1000-4-3 (class 3), IEC801-4/IEC1000-4-4
(class 4), IEC801-6/IEC1000-4-6 (class 3),
IEC1000-4-11, EN50082-2, EN60555-2

Ambient temperature

Nominal operating range +5…+45°C

(at 20…80 % atmospheric humidity)

Storage, transport –40…+70°C

Housing

Material of cover Polyurethane rigid foam (PUR) with fire protection

for fire class UL94VO, CFC-free

Material of base Steel, enamelled

Dimensions

Width 260 mm
Height 129 mm
Depth 366 mm (without dialysis cell)
Weight 5.6 kg (incl. accessories)

36

754 Dialysis Unit

5.2 Standard equipment

GL45

262

1.59

1.59

1.59

136

0.5

0.5

0.5

Subject to changes!
All dimensions are given in mm.

The 2.754.0010 Dialysis Unit includes the following parts:

Quant. Order No. Description

1 6.1602.150 Bottle attachment GL45

for 6.1608.070 clear glass bottle

1 6.1608.070 Clear glass bottle 2 L

Waste bottle, with GL45 thread

5.2 Standard equipment

GL45

1 6.1803.030 PTFE Capillary tubing

Length = 5 m

5 6.1803.040 PTFE Capillary tubing

Length = 1 m

5 6.1803.050 PTFE Capillary tubing

Length = 20 cm

1 6.1825.210 PEEK Sample loop 20 µµL

For injection valve.
Incl. two 6.2744.010 PEEK

compression fittings

2 6.1826.030 Pump tubing

made of PVC (Tygon); with 2 firmly
attached orange-yellow stoppers;
i.d. = 0.51 mm, e.d. = 2.27 mm

2 6.1826.040 Pump tubing

made of PVC (Tygon); with 2 firmly
attached black-black stoppers;
i.d. = 0.76 mm, e.d. = 2.27 mm

150

400

150

400

754 Dialysis Unit

37

5 Appendix

802821

Quant. Order No. Description

2 6.1831.040 PEEK Capillary tubing

Length = 15 cm

3 6.1831.050 PEEK Capillary tubing

Length = 40 cm

1 6.1831.060 PEEK Capillary tubing

Length = 1 m

1 6.2122.0X0 Mains cable

to customer's specifications:
Cable socket Cable connector

Type IEC 320/C 13 Type SEV 12 (CH…)............................... 6.2122.020

Type IEC 320/C 13 Type CEE (7), VII (D…)........................... 6.2122.040

Type CEE (22), V Type NEMA 5-15 (USA…) ......................6.2122.070

0.5

0.5

0.5

1.59

1.59

1.59

1 6.2621.070 Allen Key 5 mm

For the dialysis cell screws

1 6.2729.100 Dialysis cell (plexiglass)

incl. the following accessories:

1 × E.301.0111 NBR sealing ring
5 × V.022.6030 Steel screw
5 × 4.754.4090 POM washer for screw
4 × 6.2744.060 Dummy stopper for

blocking inlet and outlet
openings

1 6.2744.000 PVDF Compression fitting

For connecting 6.1803.0X0 PTFE
capillaries to the dialysis cell,
set of 5 pcs

1 6.2744.010 PEEK compression fitting

For connecting 6.1831.0X0 PEEK capillaries
and 6.1803.0X0 PTFE capillaries,
set of 5 pcs

SW5

26

1 6.2744.030 PEEK coupling

Connection between 6.2744.010 PEEK com­pression fitting and 6.1826.0X0 pump tubing ;
set of 4 pcs

38

25

754 Dialysis Unit

Quant. Order No. Description

5.3 Optional accessories

1 6.2756.000 Feedthrough

For the 733 IC Separation Center, for feeding
through capillary tubings

1 6.2831.010 Pincette

For handling dialysis membranes

1 8.754.1003 Instructions for use (English)

for 754 Dialysis Unit

5.3 Optional accessories

Order No. Description

6.2143.200 Connecting cable

Connecting cable 732 IC Detector – 754 Dialysis Unit.

12

10

120

9

6.2143.220 Connecting cable

Connecting cable 732 IC Detector – 753 Suppressor Module – 754 Dialysis Unit.

6.2714.010 Dialysis membranes

Dialysis membranes in cellulose acetate;
diameter 47 mm; thickness 115 µm; nominal pore size 0.2 µm.
set of 50 pcs

754 Dialysis Unit

39

5 Appendix

5.4 Warranty and conformity

5.4.1 Warranty

The warranty on our products is limited to defects that are traceable to
material, construction or manufacturing error which occur within 12
months from the day of delivery. In such cases the defects will be recti­fied in our workshops free of charge. Transport costs are to be paid by
the customer.

For day and night operation the warranty is limited to 6 months.
Glass breakage in the case of electrodes or other parts is not covered

by the warranty. Checks which we are asked to carry out during the
warranty period for reasons other than material or manufacturing faults
will be invoiced. For parts manufactured by third parties, insofar as
these constitute an appreciable part of our instrument, the warranty
stipulations of the manufacturer in question apply.

With regard to the guarantee of accuracy, the technical specifications in
the instruction manual are authoritative.

With regard to defects in material, construction or design as well as the
absence of guaranteed features, the purchaser has no rights or claims
except those mentioned above.

If damage of the packaging is evident on receipt of a consignment or if
the goods show signs of transport damage after unpacking, the carrier
must be informed immediately and a written damage report for re­quested. Lack of an official damage report releases Metrohm from any
liability to pay compensation.

If any instruments and parts have to be returned, the original packaging
should be used if at all possible. This applies above all to instruments,
electrodes, burette cylinders and PTFE pistons. Before embedding
them in wood shavings or similar material, the parts must be packed in
a dustproof package (for instruments the use of a plastic bag is im­perative). If open assemblies are enclosed in the scope of delivery that
are sensitive to electromagnetic voltages (e.g. data interfaces etc.)
these must be returned in the associated original protective packaging
(e.g. conductive protective bag). (Exception: assemblies with built-in
voltage source belong in a non-conductive protective packaging). No
warranty responsibility whatsoever will be accepted by Metrohm for
damage which arises as a result of non-compliance with these instruc­tions.

40

754 Dialysis Unit

5.4.2 EU Declaration of conformity

EU Declaration of Conformity

The METROHM AG company, Herisau, Switzerland hereby certifies that the in­strument:

754 Dialysis Unit

meets the requirements of EC Directives 89/336/EWG and 73/23/EWG.

5.4 Warranty and conformity

Source of the specifications

EN 50081-1 Electromagnetic compatibility, basic specification

Emitted Interference

EN 50082-2 Electromagnetic compatibility, basic specification

Interference Immunity

EN 61010 Safety requirements for electrical laboratory measurement

and control equipment

Description of the instrument

Appliance for on-line dialysis sample preparation with 2-channel peristaltic
pump

Herisau, September 27, 1997

754 Dialysis Unit

Dr. J. Frank Ch. Buchmann

Development Manager Production and

Quality Assurance Manager

41

5 Appendix

5.4.3 Certificate of conformity and system validation

Certificate of Conformity and System Validation

This is to certify the conformity to the standard specifications for electrical appli­ances and accessories, as well as to the standard specifications for security and
to system validation issued by the manufacturing company.

Name of commodity: 754 Dialysis Unit
Name of manufacturer: Metrohm Ltd., Herisau, Switzerland
Principal technical information: Voltages: 100…120, 220…240 V

Frequency: 50…60 Hz

This Metrohm instrument has been built and has undergone final type testing
according to the standards:

IEC801-2/IEC1000-4-2 (class 4), IEC801-3/IEC1000-4-3 (class 3),
IEC801-4/IEC1000-4-4 (class 4), IEC801-6/IEC1000-4-6 (class 3),
IEC1000-4-11, EN55011 (class B), EN55022 (class B), EN50081-1,
EN50082-2, EN60555-2 — Electromagnetic compatibility

IEC1010, EN61010, UL3101-1 — Security specifications

The technical specifications are documented in the instruction manual.

Metrohm Ltd. is holder of the SQS-certificate of the quality system ISO 9001 for
quality assurance in design/development, production, installation and servicing.

Herisau, September 27, 1997

Dr. J. Frank Ch. Buchmann

Development Manager Production and

Quality Assurance Manager

42

754 Dialysis Unit

5.5 Index

5.5 Index

A

Acceptor solution ..................................13,17,19,26

Air bubbles ......................................................20,29

Allen key (6.2621.070)................................ 15,32,38

Ambient temperature............................................ 36

Appendix ..............................................................35

Application Bulletins............................................... 5

Application Notes................................................... 5

Arrangement of the instruments............................. 7

Assembling the dialysis cell ................................. 16

Attaching the accessories............................... 10,13

Attaching the dialysis cell..................................... 15

B

Bacterial growth............................................... 29,32

Blockage ......................................................... 29,32

Bottle attachment 4848

Figure .............................................................18

Installation....................................................... 19

Ordering designation...................................... 37

Bottom part 3535

Figure .............................................................16

Installation.................................................. 15,32

C

Cable (6.2143.200)................................ 10,22,24,39

Cable (6.2143.220)................................ 10,22,24,39

Calibration ............................................................28

Care of the unit..................................................... 30

Caution................................................................... 5

Cell holder 66

Figure ...............................................................2

Cell volume........................................................... 35

Centrifugation....................................................... 27

Certificate of conformity and system validation ... 42

Check ..................................................................... 7

Comment................................................................ 5

Conditioning the dialysis system.......................... 20

Conformity............................................................ 41

Connecting the dialysis cell.................................. 17

Connection 2121

Figure .............................................................12

Connection to 753 Suppressor Module............... 11

Contact pressure........................................ 15,29,31

Contact pressure lever 55

Adjust contact pressure ................................. 20

Figure ..........................................................2,14

Install tubing cartridge.................................... 31

Coupling 2929

Figure ........................................................14,18

Installation.................................................. 13,14

Ordering designation...................................... 38

D

Declaration of conformity..................................... 41

Degassing............................................................ 27

Degree of protection......................................... 6,36

Diagram of tubing connections............................ 18

Dialysis cell 3434

Connection to IC system........................... 17,33

Dimantling ...................................................... 32

Figure ........................................................ 16,18

Installation ............................................ 15,16,33

Ordering designation ..................................... 38

Preparation..................................................... 15

Rinsing ........................................................... 32

Technical data................................................ 35

Dialysis flow chart ................................................ 23

Dialysis membrane 3737

Conditioning.............................................. 20,33

Figure ............................................................. 16

Handling......................................................... 30

Insertion..................................................... 15,32

Preparation................................................ 15,32

Replacement.................................................. 32

Service life...................................................... 30

Dialysis membrane (6.2714.010)............... 25,26,39

Dialysis procedure .......................................... 23,27

Dialysis time.................................................... 26,29

Dialysis with «Stopped Flow»............................... 23

Dimensions .......................................................... 36

Dismantle accessories at 733.............................. 10

Documentation....................................................... 5

Dummy stopper (6.2744.060).................... 15,28,38

E

Earthing............................................................... 6,9

Electrical safety .................................................... 10

Electromagnetic compatibility.............................. 36

EMC ..................................................................... 36

Emitted interference............................................. 36

EU Declaration of conformity............................... 41

External valve ......................................................... 1

F

Feedthrough 2222

Figure ............................................................. 12

Feedthrough 2727

Figure ............................................................. 12

Installation ...................................................... 11

Ordering designation ..................................... 39

Feedthrough 2828

Figure ............................................................. 12

Front....................................................................... 2

Fuse holder 1111

Change fuses................................................... 8

Figure ............................................................ 3,9

Fuses ............................................................. 8,9,35

754 Dialysis Unit

43

5 Appendix

G

General precautionary rules................................... 6

H

Handling dialysis membranes.............................. 30

Handling of solvents .............................................. 6

Hazard.................................................................... 5

Holding clamp 77

Figure ............................................................... 2

Install tubing cartridges.................................. 13

Housing................................................................ 36

I

IC Detector 732............................................... 22,24

Immunity to interference....................................... 36

Information about the Instructions for Use............. 4

Injection................................................................ 23

Installation ...................................................... 13

Injection valve 1818

Dismantle standard accessories.................... 10

Figure ............................................................. 12

Tubing connections........................................ 11

Injection valve 2222

Dismantle standard accessories.................... 10

Figure ............................................................. 12

Tubing connections........................................ 11

Inlet 4242

Figure ............................................................. 16

Tubing connection..................................... 17,33

Inlet 4343

Figure ............................................................. 16

Tubing connection..................................... 17,33

Installation .............................................................. 7

Installing pump tubings........................................ 14

Instructions for Use (8.754.1003)...................... 4,39

Instrument description ........................................... 1

Insufficient flow rate.............................................. 29

Insufficient yield............................................... 29,32

Interior of IC Separation Center............................ 12

Introduction ............................................................ 1

L

Leakage ............................................... 6,7,20,29,33

Location.................................................................. 7

M

Mains cable....................................................... 9,38

Mains connection........................................... 6,8,35

Mains connection plug 1616

Figure ............................................................ 3,9

Mains connection............................................. 9

Mains frequency................................................... 35

Mains switch 1313

Figure ............................................................ 3,9

Startup............................................................ 20

Switching the instrument on/off................. 21,22

Mains voltage........................................................35

Maintenance .........................................................30

Malfunctions..........................................................29

Manual operation..................................................21

Material of pump tubing........................................35

Measure standard directly....................................26

Measure standard with dialysis ............................27

Metrohm service ...................................................30

Mounting pin 1010

Figure................................................................2

Install tubing cartridges...................................13

N

Non-existing flow rate ...........................................29

Notation ..................................................................4

O

Opening the 754 Dialysis Unit ................................6

Operation..............................................................21

Operation via 732 IC Detector ..............................22

Optimum dialysis time ..........................................26

Optimum tranfer time............................................25

Optional accessories............................................39

Organization............................................................4

Outlet 4141

Figure..............................................................16

Tubing connection .....................................17,33

Outlet 4444

Figure..............................................................16

Tubing connection .....................................17,33

P

Packaging...............................................................7

Parts and controls...................................................2

PEEK capillary 1919

Figure.........................................................12,18

Installation..................................................11,19

Ordering designation......................................38

PEEK capillary 2020

Figure.........................................................12,18

Installation.......................................................11

Ordering designation......................................38

PEEK capillary 2121

Figure.........................................................12,18

Installation.......................................................11

Ordering designation......................................38

PEEK capillary 2323

Connect pump tubing.....................................13

Figure....................................................12,14,18

Installation.......................................................11

Ordering designation......................................38

PEEK capillary 2424

Connection to dialysis cell .........................17,33

Figure.........................................................12,18

Installation.......................................................11

Ordering designation......................................38

PEEK capillary 2525

Connection to dialysis cell .........................17,33

Figure.........................................................12,18

Installation.......................................................11

Ordering designation......................................38

44

754 Dialysis Unit

5.5 Index

PEEK compression fitting 2828

Figure ........................................................14,18

Installation.................................................. 13,14

Ordering designation...................................... 38

PEEK compression fitting 4747

Figure .............................................................18

Installation....................................................... 19

Ordering designation...................................... 38

PEEK sample loop 1717

Figure ........................................................12,18

Ordering designation...................................... 37

Pictograms ............................................................. 4

Pin assignment of remote interface ..................... 35

Pincette (6.2831.010) ................................. 15,32,39

Power consumption .............................................35

Precautionary rules........................................... 6

Power on lamp 33

Figure ...............................................................2

Function............................................................ 9

Ready for use display................................ 21,22

Precautionary rules................................................. 6

Prepare acceptor solution.................................... 27

Pressure ...............................................................35

Program time........................................................ 27

Program with suppression ................................... 24

Program without suppression.............................. 24

Programming with 732 IC Detector...................... 24

Protection class.............................................. 6,9,36

Protective earth ...................................................... 9

PTFE capillary 2626

Figure ........................................................14,18

Installation....................................................... 13

Ordering designation...................................... 37

PTFE capillary 2727

Figure ........................................................14,18

Installation....................................................... 14

Ordering designation...................................... 37

PTFE capillary 3333

Connection of pump tubing ...........................14

Connection to dialysis cell......................... 17,33

Figure ........................................................14,18

Ordering designation...................................... 37

PTFE capillary 4545

Connection to dialysis cell......................... 17,33

Figure .............................................................18

Installation....................................................... 19

Ordering designation...................................... 37

Pump capacity .....................................................35

Pump drive 99

Figure ...............................................................2

Pump tubing

Installation....................................................... 13

Precautionary rules........................................... 6

Pump tubing 3030

Figure ........................................................14,18

Ordering designation...................................... 37

Replacement .................................................. 31

Service life ......................................................31

Pump tubing 3131

Figure ........................................................14,18

Ordering designation...................................... 37

Replacement .................................................. 31

Service life ......................................................31

Pump type............................................................ 35

PVDF compression fitting 4646

Figure .............................................................18

Installation....................................................... 19

Ordering designation...................................... 38

PVDF compression fitting 5050

Figure ............................................................. 18

Installation ...................................................... 17

Ordering designation ..................................... 38

R

Rear........................................................................ 3

Rear panel opening 4141

Figure ............................................................. 12

Recovery rate....................................................... 26

Rectification of malfunctions................................ 29

Remote interface 1515

Figure ............................................................... 3

Technical data................................................ 35

Remote operation via 732 IC Detector ................ 10

Replacing the dialysis membrane ....................... 32

Replacing the pump tubing ................................. 31

Rinsing ............................................................ 23,31

Rinsing dialysis system........................................ 28

Rinsing time ......................................................... 25

S

Safety notes ...................................................... 6,36

Safety specifications ............................................ 36

Sample.................................................. 14,17,19,23

Sample determination.......................................... 28

Sample determinations with dialysis.................... 27

Sample preparation ............................................. 27

Screw 3939

Dismantling dialysis cell................................. 32

Figure ............................................................. 16

Installation ...................................................... 15

Ordering designation ..................................... 38

Sealing ring 3636

Figure ............................................................. 16

Installation ................................................. 15,32

Ordering designation ..................................... 38

Serial number 1414

Figure ............................................................... 3

Service life of pump tubing .................................. 15

Servicing............................................................... 30

Setting the dialysis time ....................................... 26

Setting the mains voltage.................................... 8,9

Setting the program time ..................................... 27

Setting the rinsing time......................................... 25

Setting the transfer time....................................... 25

Setting up the instrument....................................... 7

Shutdown of the dialysis system .................... 28,31

Slot 4040

Figure ............................................................. 16

Snap-action lever 88

Figure .......................................................... 2,14

Install tubing cartridges.................................. 13

Standard equipment ............................................ 37

Starting up the 754 Dialysis Unit.......................... 20

Static charges ........................................................ 6

Stopped-Flow procedure....................................... 1

Stopper 3232

Figure ............................................................. 14

Install pump tubing ................................... 13,31

754 Dialysis Unit

45

5 Appendix

Storage................................................................. 36

Support documentation......................................... 5

Suppressor Module 753 ...................................... 11

Switch instrument on/off............................... 9,21,22

Switch off remote control ..................................... 21

Switch on remote control ..................................... 22

Switch pump on/off at an event ........................... 22

Switch pump on/off in a program ........................ 22

Switch pump on/off.............................................. 21

Switch 11 <PUMP>

Figure ............................................................... 2

Function ......................................................... 21

Startup............................................................ 20

Switch 22 <REMOTE>

Figure ............................................................... 2

Function .................................................... 21,22

Startup............................................................ 20

T

Technical data...................................................... 35

Top part 3838

Figure ............................................................. 16

Installation ...................................................... 15

Total ion concentration......................................... 26

Transfer time ................................................... 25,29

Transfer to sample loop....................................... 23

Transport................................................................ 7

Transport damages........................................... 7,40

Tubing cartridge 44

Figure .......................................................... 2,14

Install pump tubings....................................... 13

Installation ...................................................... 31

Tubing connections for acceptor solution ...... 13,17

Tubing connections for sample ...................... 14,17

Tubing connections for valve A and B ................. 11

V

Voltage selection insert 1212

Change fuses................................................... 8

Figure ............................................................ 3,9

W

Warning .................................................................. 5

Warranty ............................................................... 40

Washer (4.754.4090)....................................... 16,38

Waste bottle 4949

Attaching the accessories.............................. 19

Figure ............................................................. 18

Ordering designation ..................................... 37

46

754 Dialysis Unit