Eppendorf S41i User Manual
Operating manual
—
S41i CO2 Incubator Shaker
see
on p.Fig.Tab.p
.
Op
l
erating manua
October 15, 2012
New Brunswick CO2 Incubator Shaker
S41i
Operating manual
M1334-0050
Revision B
Copyright
Copyright© 2013 Eppendorf AG, Germany. No part of this publication may be reproduced without the prior permission of
the copyright owner.
The company reserves the right to change information in this document without notice. Updates to information in this
document reflect our commitment to continuing product development and improvement.
Trademarks
Eppendorf® and the eppendorf logo are registered trademarks and New Brunswick and the New Brunswick logo are
trademarks of Eppendorf AG, Germany.
BioCommand® is a registered trademark of New Brunswick Scientific Co., Inc., USA.
Windows
®
is a registered trademarks of Microsoft Corporation in the United States and other countries.
Microsoft® and Excel® are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or
other countries.
Trademarks are not marked in all cases with ™ or ® in this manual.
Eppendorf has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are
unintentional.
January 28, 2013
Revision B
M1334-0050
S41i CO2 Incubator Shaker — Operating manual
Table of contents
1 Table of contents
1
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.1 Main illustration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.2 Application limits. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.2.1 Description of ATEX Guideline (94/9EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.4.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.1 Inspection of boxes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.2 Unpacking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.3 Utilities requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.4 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4.5 Installation of foot shields . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.6 Platform assemblies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.7 Installation of platform. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.8 Flask clamp installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.9 Stacking instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.10 Electrical connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.11 Setting up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.12 Making connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4.13 Ethernet connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Table of contents
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.1 Preparing for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.2 Using the humidity trays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.3 Starting the incubator/shaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
5.4 Using the touchscreen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.4.1 Initial display and STATUS screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
5.4.2 Starting the platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
5.4.3 Setting the temperature. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
5.4.4 Setting the CO
5.4.5 Setting the shaking speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5.4.6 % RH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
5.5 MENU 1 screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.5.1 HELP function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.5.2 EVENT LOG . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.5.3 ALARM SETTINGS screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.5.4 SUMMARY screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
level. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
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Table of contents
S41i CO2 Incubator Shaker — Operating manual
5.5.5 EVENT GRAPH screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5.6 SET CALIBRATION screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.5.7 GENERAL SETTINGS screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
5.5.8 USER ACCESS screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.5.9 COMMUNICATIONS screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.6 MENU 2 screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.6.1 SERVICE screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.6.2 DIAGNOSTICS screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.6.3 MAINTENANCE screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.6.4 OPTIONS screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
5.6.5 SET CO
5.6.6 DISINFECTION screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
5.7 Mains/Power interruption . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1.1 General notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1.2 Daily checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1.3 Weekly checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1.4 Monthly check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1.5 Other checks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.1.6 CO
6.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.3 Disinfection/Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.4 Product return. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
AUTOZERO screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
2
Sampling with Analyzer. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
2
7 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.1 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.1.1 Device dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.1.2 Platform dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.2 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.2.1 Speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.2.2 CO
8.2.3 Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
8.2.4 Passive humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
8.3 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
9 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
9.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
10 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
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S41i CO2 Incubator Shaker — Operating manual
1 Operating instructions
1 Operating instructions
1.1 Using this manual
Carefully read this operating manual before using the device for the first time.
Also observe the operating manual enclosed with the accessories.
The operating manual should be considered as part of the product and stored in a location
that is easily accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website http://newbrunswick.eppendorf.com
1.2 Danger symbols and danger levels
1.2.1 Hazard symbols
Hazard point Burns
Electric shock Material damage
.
1
Operating instructions
Explosion Heavy loads
Inhalation Crush
1.2.2 Degrees of danger
The following degree levels are used in safety messages throughout this manual. Acquaint
yourself with each item and the potential risk if you disregard the safety message.
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
1.3 Symbols used
Example Meaning
You are requested to perform an action.
1.
2.
• List.
Perform these actions in the sequence described.
References useful information.
5
1
S41i CO2 Incubator Shaker — Operating manual
1.4 Abbreviations used
kWh Kilowatt hours
LEL Lower Explosion Limit
rpm Revolutions Per Minute
UEL Upper Explosion Limit
VA Volt Amps
VAC Voltage in Alternating Current
Operating instructions
6
S41i CO2 Incubator Shaker — Operating manual
2 Product description
2 Product description
2.1 Main illustration
Abb. 1: Front and rear views
2
Product description
Fig. 1: Front and rear views
1 Display 3 Control box
2 Door handle 4 Foot shield
2.2 Delivery package
The operating manual and mains/power cord are shipped on top of the control box at the rear of
Hint!
the incubator/shaker.
Quantity Item Notes
1 Perforated Stainless Steel Shelf Installed
1 White porous CO
Sensor Cover
2
1 Black Sensor Cover
1 Front foot shield Packed on top of incubator/
2 Side foot shields
2 Humidity Trays Packed in box on shelf
3m(9.8ft) PVC Tubing, ~6mm(1/4in) bore, with an
inline CO
HEPA-filter connected, ready for
2
use
2 Hose Clips Packed in humidity tray
shaker
Packed in box on shelf
1 Auto-zero HEPA Filter
1 Mains/power Cord Packed on top of control box at
1 Operating Manual
rear of incubator/shaker
7
2
S41i CO2 Incubator Shaker — Operating manual
Risk of silica particulates transferring to skin
Wear gloves anytime you touch the white CO
CAUTION!
Do not later touch those gloves to your face.
Discard or wash the gloves.
2.3 Features
The chamber’s fanless design with six-sided convection heating provides gentle circulation and
uniform temperature. In addition, the seamless one-piece chamber eliminates area where
contaminants could collect and makes wipe-down easy.
Two humidity trays reduce sample evaporation.
The New Brunswick S41i fits on the floor, on the floor under a work bench or it can be double
stacked to save space. The shaking mechanism is a heavy-duty drive with three eccentric drive
shafts to provide stability, uniform motion and assure long incubator/shaker life. The orbit is
2.5cm (1 in) and the shaking speed range is from 25-400rpm(±1rpm), unless two are
stacked, in which case the maximum speed of the top incubator/shaker is limited to 250rpm.
With no shelf present, flasks up to 4L in size can be installed on the shaking platform. With one
stationary shelf present, Erlenmeyer flasks up to 1L can be installed. If two shelves are present,
Erlenmeyer flasks, because of their tall necks, must be limited to 250mL.
The temperature range is from 4°C above ambient temperature to 50°C. Temperature accuracy
is ±0.2°C through the entire range.
Product description
CO2 control ranges from 0.2to20%, with an accuracy of ±0.1% at 20% CO2 via the InfraRed
CO2 sensor.
sensor cover.
2
8
S41i CO2 Incubator Shaker — Operating manual
3 Safety
3Safety
3.1 Intended use
The New Brunswick S41i is half CO2 incubator and half biological shaker, combining the best
features of New Brunswick incubators and shakers into one versatile instrument. It provides
gas concentrations, enabling growth of
2
CAUTION!
gentle shaking and accurate control of temperature, CO
hybridomas, mammalian and stem cells, insect cultures, as well as aerobic and anaerobic
bacteria and yeast. A perforated stationary shelf is included for plates and T-flasks to enable
simultaneous incubation of adherent cultures.
The New Brunswick S41i was designed for controlled incubation and shaking of biological
samples. Do not use any heat-generating device inside the chamber.
Lack of safety due to incorrect accessories or spare parts
Accessories and spare parts that are not recommended by Eppendorf compromise the safety,
function and precision of the device. Eppendorf cannot be held liable or accept any liability for
damage resulting from the use of non-recommended accessories and spare parts.
Only use accessories and original spare parts recommended by Eppendorf.
3
When one shelf is installed, the largest flasks that can be contained in this incubator/shaker are 1
Hint!
L Erlenmeyer and 2.8 L Fernbach flasks. Without a shelf, Erlenmeyer flasks up to 4 L can be
contained.
3.2 Application limits
3.2.1 Description of ATEX Guideline (94/9EC)
Explosion hazard
Do not operate the device in areas where work is completed with explosive substances.
DANGER!
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
Due to its design and the ambient conditions in its interior, the device is not suitable for use in
potentially explosive atmospheres.
The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated
lab.
The use of substances which may contribute to a potentially explosive atmosphere is not
permitted.
The final decision regarding the risks associated with using these types of substances is the
user's responsibility.
3.3 Information on product liability
Safety
In the following cases, the designated protection of the device may be compromised.
The liability for the function of the device passes to the operator if:
• The device is not used in accordance with this operating manual.
• The device is used outside of the range of application described in the succeding chapters.
• The device is used with accessories or consumables that were not approved by Eppendorf.
• Service or maintenance is completed on the device by people who are not authorized by
Eppendorf.
• The owner has made unauthorized modifications to the device.
9
3
S41i CO2 Incubator Shaker — Operating manual
3.4 Warnings for intended use
Before using the incubator/shaker, read the operating manual and observe the following general
safety instructions.
3.4.1 Personal injury and damage to device
Risk of personal injury
Burns due to hot surface.
WARNING!
CAUTION!
NOTICE!
Do not touch the incubator/shaker during the high temperature disinfection cycle.
Do not open incubator/shaker door during the cycle.
Risk of personal injury
A mechanical lifting device is required to safely lift the incubator/shaker.
Risk of material damage
Never try to lift the incubator/shaker by its door; this would cause permanent damage to the
incubator/shaker.
Never lean on or place objects on the open door.
Safety
NOTICE!
Risk of material damage
To avoid possible damage to the CO
incubator/shaker is switched off, or when a high temperature disinfection cycle is initiated.
sensor, never leave water in the humidity trays while the
2
Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor removal (optional
feature).
10
S41i CO2 Incubator Shaker — Operating manual
4 Installation
4 Installation
4.1 Inspection of boxes
After you have received your order, inspect the boxes carefully for any damage that may have
occurred during shipping. Report any damage to the carrier and to your local Eppendorf
distributor immediately.
4.2 Unpacking
Risk of personal injury
A mechanical lifting device is required to safely lift the incubator/shaker.
CAUTION!
Risk of material damage
Never try to lift the incubator/shaker by its door; this would cause permanent damage to the
NOTICE!
incubator/shaker.
Never lean on or place objects on the open door.
Save the packing materials for possible future use, and be sure to save this Operating Manual
for instruction and reference. User manuals can also be found online, in PDF format, at
www.nbsc.com.
Save the foot shields packed on top of the incubator/shaker, which you will install.
Use a mechanical lifting device to safely lift the incubator/shaker off its shipping pallet and to
transport it to the installation site (see Location on p. 12). You may push the incubator/shaker
a short distance using the built-in castors to roll it. To do this, you may need to rotate the
incubator/shaker feet to raise them so the incubator/shaker will rest on its castors.
Remove the sleeved box sitting on the top shelf inside the incubator/shaker. As indicated in
Delivery package (see Delivery package on p. 7), It contains the two humidity trays, PVC
tubing and several other essential parts.
Remove the foam blocks from the top shelf and save them with the other packing materials.
Locate and remove the parts stored in the humidity trays.
Locate and unpack any other components.
If any part of your order was damaged during shipping, is missing, or fails to operate, please
contact your Eppendorf distributor.
Using your packing list, verify that you have received the correct materials, and that nothing is
missing.
4
Installation
4.3 Utilities requirements
The following utilities requirements are needed for operation:
Utility Requirement
Electricity 120V,50/60Hz earthed/grounded mains/electrical supply
230V,50/60Hz earthed/grounded mains/electrical supply
gas Cylinder with 100% CO2 vapor withdrawal
CO
2
CO2 gas pressure to device must exceed 7PSI (0.5bar) for optimal operation.
Hint!
11
S41i CO2 Incubator Shaker — Operating manual
4.4 Location
The incubator/shaker is designed to operate at a chamber temperature of 4.0°C above ambient.
If the incubator/shaker is being operated at 37°C, the absolute minimum ambient temperature
recommended is 18°C. Maximum allowable ambient temperature is 25°C.
Position the incubator/shaker, allowing enough space to open the door and to access the CO2
Hint!
sample port on the right side of the incubator/shaker.
Care should be taken to avoid placing the incubator/shaker in a position that may affect its
performance, such as those listed below.
DO NOT place the incubator/shaker:
• Directly under, beside or within the air flow of heating or air-conditioning ducts, or other drafts
• Directly beside heat-generating equipment such as a heater, an autoclave or an oven
• Near the exhaust of heat- or cold-generating equipment (like an ultra-low temperature
freezer)
• Near a window exposed to direct sunlight
• Directly on top of any heat-generating apparatus
• Without minimum ventilation clearance of 10mm (0.5in) all around
4
Installation
NOTICE!
Abb. 2: Adjustable feet
Risk of material damage
Never try to lift the incubator/shaker by its door; this would cause permanent damage to the
incubator/shaker.
Never lean on or place objects on the open door.
Remove the incubator/shaker from the pallet as described in Unpacking (see Unpacking on
p. 11).
Place the incubator/shaker in the working position, on a level floor capable of bearing its
weight. You may need to rotate the incubator/shaker feet to raise them, in order to rest the
incubator/shaker on its castors to be moved more easily (see Fig. 2 on p. 12).
Fig. 2: Adjustable feet
1 Castors located in all four corners 3 Lock nut
2 Adjustable foot
12
S41i CO2 Incubator Shaker — Operating manual
The weight of the incubator/shaker is approximately 152kg(335lb), but actual in-use weight will
naturally be heavier and will depend on both the options installed and the material stored in the
incubator/shaker.
The incubator/shaker is designed so that one incubator/shaker can be safely stacked on top of
another S41i using the optional stacking kit, which includes instructions. It is not possible to put
any other type of incubator/shaker or heavy apparatus on top, as the top cover and stacking kit
are designed to support only the feet of another S41i.
4.5 Installation of foot shields
The front and side foot shields (see Fig. 3 on p. 13) are packed on top of the incubator/shaker,
enclosed in bubble wrap. To install them, beginning with the right side foot shield, follow this
procedure:
1. Orient the mounting bracket at the front face of the incubator/shaker, then align the foot
Abb. 3: Front and side foot shields
shield’s two keyhole slots with the two mounting pins on the bottom of the incubator/shaker.
Fig. 3: Front and side foot shields
Abb. 4: Side foot sh ield installation
4
Installation
Fig. 4: Side foot shield installation
1 Right side of incubator/shaker 4 Hole for front foot shield’s ball catch
2 Keyhole slot on right side foot shield 5 Hole for M4 screw
3 Mounting bracket
13
4
S41i CO2 Incubator Shaker — Operating manual
2. Snap the side shield in place, then slide it toward the back of the incubator/shaker until the
bracket is flush against the front face.
3. Fasten the bracket to the incubator/shaker with one M4 screw:
Abb. 5: Side foot sh ield bracket
Fig. 5: Side foot shield bracket
1 M4 screw inserted here to fasten the side foot shield bracket to the incubator/shaker
4. Repeat Steps 1 through 3 for the left side foot shield.
5. Push fit the remaining foot shield onto the bottom front of the incubator/shaker. Be sure to
align its pins with the holes on the incubator/shaker and on the mounting brackets of the side
foot shields.
Abb. 6: Front foot shield installation
Installation
Fig. 6: Front foot shield installation
4.6 Platform assemblies
4.7 Installation of platform
A platform, which is required for operation, is a separate item, not included with the shaker
assembly. The S41i platform, measuring 612×356mm(24×14in), will accept a wide range of
clamps for flasks, test tubes, etc.
Prior to use, a platform must be installed on the incubator/shaker.
Hint!
The incubator/shaker is shipped with four Allen head platform screws installed in the four bearing
housing uprights, as shown below:
14
S41i CO2 Incubator Shaker — Operating manual
Abb. 7: Installing plat form
Fig. 7: Installing platform
1 Glass inner door 5 Bearing housing
2 Allen screws 6 Bearing housing uprights
3 Platform 7 Top shelf, shipped installed
4Humidity trays
1. Set the mains/power to OFF and unplug the incubator/shaker.
2. Remove the platform screws, then use them to install the platform over the bearing housing.
4.8 Flask clamp installation
Flask clamps purchased for use with the platform require installation. Clamps are installed by
securing the base of the clamp to the platform with the correct type and number of screws. All
clamps are shipped complete with hardware.
The S41i platform requires 10-24×5/16-inch Phillips-head screws (which are supplied) to fasten
Hint!
flask clamps.
With one shelf installed, Erlenmeyer flasks larger than 1L are too tall for the chamber; to use
2L–4L Erlenmeyer flasks, remove the shelf.
Clamps for 2.8-liter Fernbach flasks and 2L–4L Erlenmeyer flasks are shipped with an
additional girdle to keep the flasks in place. The girdle is an assembly of springs and sections of
rubber tubing. One girdle is already in place on the clamp, the other is packed separately.
To install these double girdle clamps:
1. Place the clamp on the platform, aligning its mounting holes with holes on the platform.
Secure the clamp in place using the flat Phillips head screws provided
(#S2116-3051,10-24x5/16-inch).
4
Installation
Three different types of screws are shipped with the clamps, to identify the proper screws
Hint!
(see Fig. 8 on p. 16).
2. With the first girdle in place, as delivered, on the upper part of the clamp body, insert an
empty flask into the clamp.
15
S41i CO2 Incubator Shaker — Operating manual
3. After making sure the sections of tubing are located between the clamp legs, roll the first
girdle down the legs of the clamp as far as it can go. The tubing sections will rest against the
platform, and the springs will be under the clamp base.
4. Place the second girdle around the upper portion of clamp body (just as the first girdle was
initially). Make sure that its spring sections rest against the clamp legs, while its rubber tubing
sections sit against the flask, in between the clamp legs.
Abb. 8: Clamp fastener
Fig. 8: Clamp fastener
New Brunswick flask clamps are used on a variety of incubator/shaker platforms. Flat head
screws of different lengths and thread pitch are used to secure the clamp. The following table
identifies the proper screw for your incubator/shaker application by reference to the head style.
Select the appropriate screws and set the others aside.
Description Part number Qty Application
S2116-3051 1 5/16"(7.9mm) thick aluminum, phenolic
10-24×5/16(7.9mm) flat Phillips
(+) head screw
and stainless steel platforms.
4
Installation
One-liter and larger flask clamps are fastened with 5 screws.
Hint!
Abb. 9: Double gird le clamp installatio n
Fig. 9: Double girdle clamp installation
1 Upper girdle with girdle tubes 3 Lower girdle with girdle tubes
2 Clamp body (legs and base) 4 Clamp mounting holes (quantity:5)
The upper girdle secures the flask within the clamp, and the bottom girdle keeps the flask from
Hint!
spinning.
4.9 Stacking instructions
16
To stack two S41i incubator shakers, you must first have purchased the stacking kit. You will find
the instructions contained in the kit.
S41i CO2 Incubator Shaker — Operating manual
4.10 Electrical connections
Risk of electrical hazard
Before making electrical connections, be sure to check the following list.
CAUTION!
1. If you have not already done so, check that the voltage and frequency of your incubator/
shaker are compatible with your mains/electric supply.
2. Set the circuit breaker on the left side of the incubator/shaker to the OFF position.
ONLY THEN:
Risk of material damage
An earthed/grounded electrical outlet is necessary for the safe operation of this instrument.
NOTICE!
3. Plug the mains/power cord into an earthed/grounded electrical outlet.
4.11 Setting up
1. If necessary, level the incubator/shaker by adjusting the feet, after removing the foot shields.
Place a small level on the shelf of the incubator/shaker. Adjust the leveling feet until the
incubator/shaker is level and stable. Lock the leveling feet in place by tightening the locking
nuts on each foot. Reinstall the foot shields.
2. Fill each humidity tray halfway with water (approximately 250mL) if you wish to use them for
humidification.
4
Installation
Hint!
Abb. 10: Installing the humidity trays
Be sure to install the humidity trays one at a time, after wiping the bottom and edges dry.
If you will not use them for humidification, install them anyway to catch spills.
3. Place the humidity trays one at a time onto the parallel support rods on either side of the
bearing housing at the bottom of the incubator/shaker. Sit them behind the stopper bends,
making sure that the back edge of each tray fits under the end of the condensation/spill
channel.
Fig. 10: Installing the humidity trays
17
4
S41i CO2 Incubator Shaker — Operating manual
Abb. 11: Humidity trays, installed
Fig. 11: Humidity trays, installed
4.12 Making connections
Risk of explosion
Use gases in this equipment only within the range between their lower explosion limit (LEL)
WARNING!
and their upper explosion limit (UEL).
If your process requires or produces gases, be sure to verify their LEL and UEL concentration
range (available online or ask your gas supplier).
Installation
In-line regulators are provided on the back of the incubator/shaker. These are essential to proper
Hint!
operation.
1. Connect the incubator/shaker to the CO2 supply using the ~6mm plastic tubing (with
installed HEPA filter) by attaching the tubing from the supply to the top regulator inlet on the
back of the incubator/shaker. The bottom regulator inlet is reserved for N2 if your incubator/
shaker is equipped with the oxygen control option.
18
S41i CO2 Incubator Shaker — Operating manual
Abb. 12: Rear view (back panel rem oved)
Fig. 12: Rear view (back panel removed)
1 ON/OFF switch 4 Inline gas regulators (access from the side)
2 Door handle 5 Access port
3 See Control box (right side) figure for details.
2. Use the tubing clips provided to eliminate CO
Risk of electrical hazard
leaks.
2
Before making electrical connections, verify that your mains/power supply voltage matches
WARNING!
the voltage of your incubator/shaker and that the on/off switch is in the OFF position.
3. Plug the mains/power cord into its receptacle on the right side of the control box.
4. After verifying that your supply voltage matches the voltage specified for your incubator/
shaker and that the ON/OFF switch is OFF, plug the cord into your mains/power supply outlet.
5. Press the Auto-Zero HEPA filter gently into the white plastic filter socket on the right side of
the control box.
4
Installation
19
S41i CO2 Incubator Shaker — Operating manual
Abb. 13: Control box (right side)
4
Installation
Fig. 13: Control box (right side)
1 Door handle 4 Auto Zero filter socket
2 Adjustable foot (shown with side shield) 5 Back panel in place
3 Mains/power receptacle
Abb. 14: Control box (left side)
Fig. 14: Control box (left side)
1 (Shown with back panel removed) 3 Adjustable foot (shown without side shield)
2 Ethernet connection
20
S41i CO2 Incubator Shaker — Operating manual
4.13 Ethernet connection
The ethernet connection is provided as a connection to a personal computer on which you can
run New Brunswick BioCommand
from its operation. Speak to your sales representative for information about this program.
®
SFI software to control the incubator/shaker and log data
4
Installation
21
5
Operation
S41i CO2 Incubator Shaker — Operating manual
5 Operation
5 Operation
5.1 Preparing for operation
1. Remove the black protective cover from the CO2 sensor, and store it in a safe place. The
sensor cap should be placed back on the sensor when the incubator/shaker is to be cleaned.
2. Ensure that the white porous sensor cover remains in place.
Risk of silica particulates transferring to skin
CAUTION!
Wear gloves anytime you touch the white CO
Do not later touch those gloves to your face.
Discard or wash the gloves.
3. Using the mains/power cord provided, connect the incubator/shaker to a earthed/grounded
mains/power supply.
4. Switch the incubator/shaker ON using the on/off switch at the top left side of the incubator/
shaker.
The display will illuminate immediately.
sensor cover.
2
5. Turn on the CO
6. The chamber setpoints are pre-programmed at 37.0°C and 5% CO
shaker on until the programmed chamber temperature and CO2 concentration have been
reached.
• The incubator shaker’s CO
Hint!
temperature setpoint. After the temperature setpoint is reached, the CO
allowing the incubator/shaker to reach the CO2 setpoint.
• If mains/power is interrupted to the incubator/shaker long enough for the temperature to drop
below setpoint, the CO
(This serves to avoid spurious CO2 readings while the incubator/shaker is below its
temperature setpoint)
7. Leave the incubator/shaker running overnight to allow conditions to stabilize.
5.2 Using the humidity trays
If humidification is required, each of the two humidity trays should be filled with approximately
250mL of warm (~37.0°C) distilled water at this time.
Risk of material damage
To avoid possible damage to the CO
NOTICE!
incubator/shaker is switched off, or when a high temperature disinfection cycle is initiated.
Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor removal (optional
feature).
gas supply with the pressure regulator set to 7 PSI (0.5 bar).
2
. Leave the incubator/
2
valve is disabled until the incubator/shaker reaches the
2
valve will be deactivated until temperature setpoint is again achieved.
2
sensor, never leave water in the humidity trays while the
2
valve is activated,
2
The humidity level within the chamber is not adjustable. The internal chamber will reach between
85 and 95% relative humidity at 37°C (depending on ambient humidity) using the humidity
trays.
5.3 Starting the incubator/shaker
The incubator/shaker will not operate if the door is open.
Hint!
To initially start the incubator/shaker, close the door, make sure the incubator/shaker’s mains/
power cord is securely plugged in, and turn the mains/power switch (located on the top left side of
the instrument) to the ON (I) position.
22
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