Instructions for useS-4-72/S-4-104seeon p.Fig.Tab.p.
English (EN)Instructions for use
Instructions for use
S-4-72/S-4-104
English (EN)
This document supplements the operation manual Centrifuge 5804/5804 R/5810/5810 R
and does not replace it. Therefore, please also read the operating manual before starting
up the rotors for the first time. You can find the current version on the Internet at
www.eppendorf.com.
1 Product description
The rotor S-4-72 is a swing-bucket rotor with a maximum capacity of 4 × 250 mL for the
Centrifuge 5804/5804 R/5810/5810 R.
The rotor S-4-104 is a swing-bucket rotor with a maximum capacity of 4 × 750 mL for the
Centrifuge 5810/5810 R. This rotor has also been designed for centrifugation of Deepwell
plates, microplates and PCR plates in plate buckets.
2 Safety notes
NOTICE! If handled incorrectly, the rotor can fall over.
The rotor can fall over if the buckets are used as a handle.
Remove the buckets before inserting and/or removing a swing-bucket rotor.
Always use both hands to carry the rotor cross.
Rotor S-4-104 only
CAUTION! Risk of injury due to chemically damaged rotor lids or caps.
Transparent rotor lids or caps made from PC, PP or PEI may loose their strength
under the impact of organic solvents (e.g. phenol, chloroform).
If rotor lids or caps have come into contact with organic solvents, they should
be cleaned immediately.
Regularly check the rotor lids and caps for damages and cracks.
Replace rotor lids or caps which show cracks or milky stains immediately.
WARNING! Damage to health due to rotor lids and caps which are not
aerosol-tight.
The aerosol tightness of rotor lids and caps is affected by autoclaving,
mechanical strain and contamination by chemicals.
Check the integrity of the seals of the aerosol-tight caps before each use.
Only work with undamaged and clean seals.
Do not close aerosol-tight rotors and buckets for storing them.
Instructions for use
2
S-4-72/S-4-104
English (EN)
3 Centrifuging
Follow the notes in the operating manual of the centrifuge on inserting the rotor,
symmetric loading of buckets, max. load per bucket and the max. loading height/
tube length of the buckets.
3.1 Rotor S-4-104 only
NOTICE! Buckets swinging out in the wrong direction.
When working with the rotor S-4-104, using the wrong adapters with 500 mL
Corning tubes may lead to the loss of samples or damage to the centrifuge.
Only use the Eppendorf adapter intended for this purpose.
Inserting and removing the rotor S-4-104
To insert and remove the rotor S-4-104, use the rotor key supplied with the rotor. This
rotor key is different from the one in the delivery package of Centrifuge 5810/5810 R.
Loading 750-mL round buckets
To minimize imbalance align the adapters in the 750-mL round bucket symmetrically as
follows.
Turn the adapter in the 750-mL round bucket to the position where the markings on
the adapter and the buckets are aligned.
Closing the bucket with the cap
NOTICE! Damage to the cap hook.
If the cap is not fitted correctly on the bucket the clamp may break during
closing.
Before you fold the clamp, check that the cap is positioned correctly.
Instructions for use
S-4-72/S-4-104
English (EN)
1. Fold the cap clamp to the open position
(1).
2. Place the cap on the bucket and push the
cap down so that the clamp is lifted
slightly (2).
3. To transport the bucket, fold the clamp to
the carrying position (3).
4. Fold the clamp beyond the latch into the
close position.
The clamp has only been folded correctly
if there is an audible click (4).
Transporting the bucket
When the bucket is closed you can lift it up with the clamp and transport it. Aerosol
tightness will also be maintained in the carrying position.
1. Fold the clamp into the upright position (carrying position) (3).
2. Lift the bucket with the clamp.
Transport the bucket carefully in the carrying position. Do not swing it
backwards and forwards, as aerosol tightness will not be maintained.
3
2
1
3
4
Opening the bucket
Fold the clamp to the open position (1).
When the clamp is pushed down the cap will lift slightly and the cap can be lifted off
the bucket.
As the air cools down in the bucket, it can generate a vacuum in the bucket.
When you open the cap, fold the clamp fully to the open position.
The clamp acts as a lever and lifts the cap off the bucket.
Instructions for use
4
S-4-72/S-4-104
English (EN)
3.2 Replacing the cap seal
The seals of aerosol-tight caps can be replaced. Proceed as follows:
Removing the seal
1. Lift the rubber seal from the groove
using a blunt lever. You can, for instance,
use the rounded side of a paper clip.
Please take care not to damage the seal
with the wire ends.
2. Carefully pull the seal out of the groove.
3.3 Inserting the seal
NOTICE! Faulty sealing when the seal is handled incorrectly.
Uniformly insert the seal.
Do not pull the seal lengthwise.
1. Check the integrity of the seal.
Do not use any damaged, discolored or dirty seals.
2. Place the seal over the groove and press the seal slightly into the groove.
3. Place the cap on the bucket and close the cap fully.
4. Remove the cap and check that the seal is seated correctly.
If the seal is too long or too short remove it from the groove and repeat the
insertion process.
3.4 Retrofitting older centrifuges
The rotors can be operated in the devices from software version 8.4 onwards. Older
devices can be retrofitted from software version 7.0 onwards. You can view the software
version on your device’s display shortly after switching on the device.
Retrofitting may only be carried out by a trained service technician. If you require
retrofitting, please contact your dealer.
4 Technical data
Rotor S-4-104
Max. g-force (rcf)/speed (rpm):
Round bucket 750 mL 3,214 x g/3,900 rpm
Max. loading height / tube length:
Round bucket 750 mL
Please note the technical data in the
operating manual of the centrifuge.
Max. load per bucket:
(Adapter, tube and contents)
Round bucket 750 mL 1000 g
Max. centrifugation radius:
Round bucket 750 mL 18.9 cm
Rotor S-4-72
Max. g-force (rcf)/speed (rpm):
Round bucket 250 mL 3,234 x g/4,200 rpm
Max. loading height / tube length:
Round bucket 250 mL
Please note the technical data in the
operating manual of the centrifuge.
Max. load per bore:
(Adapter, tube and contents)
Round bucket 250 mL 500 g
Max. centrifugation radius:
Round bucket 250 mL 16.4 cm
Instructions for use
S-4-72/S-4-104
English (EN)
Please refer to the operating manual for more technical data and order numbers.
Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
©2013 · Eppendorf
5820 740.965-02/092013
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