Eppendorf S-4-104 User Manual

Instructions for useS-4-72/S-4-104seeon p.Fig.Tab.p.

English (EN)Instructions for use

Instructions for use

S-4-72/S-4-104
English (EN)

This document supplements the operation manual Centrifuge 5804/5804 R/5810/5810 R
and does not replace it. Therefore, please also read the operating manual before starting
up the rotors for the first time. You can find the current version on the Internet at
www.eppendorf.com.

1 Product description

The rotor S-4-72 is a swing-bucket rotor with a maximum capacity of 4 × 250 mL for the
Centrifuge 5804/5804 R/5810/5810 R.

The rotor S-4-104 is a swing-bucket rotor with a maximum capacity of 4 × 750 mL for the
Centrifuge 5810/5810 R. This rotor has also been designed for centrifugation of Deepwell
plates, microplates and PCR plates in plate buckets.

2 Safety notes

NOTICE! If handled incorrectly, the rotor can fall over.

The rotor can fall over if the buckets are used as a handle.

 Remove the buckets before inserting and/or removing a swing-bucket rotor.
 Always use both hands to carry the rotor cross.

Rotor S-4-104 only

CAUTION! Risk of injury due to chemically damaged rotor lids or caps.

Transparent rotor lids or caps made from PC, PP or PEI may loose their strength
under the impact of organic solvents (e.g. phenol, chloroform).

 If rotor lids or caps have come into contact with organic solvents, they should

be cleaned immediately.

 Regularly check the rotor lids and caps for damages and cracks.
 Replace rotor lids or caps which show cracks or milky stains immediately.

WARNING! Damage to health due to rotor lids and caps which are not
aerosol-tight.

The aerosol tightness of rotor lids and caps is affected by autoclaving,
mechanical strain and contamination by chemicals.

 Check the integrity of the seals of the aerosol-tight caps before each use.
 Only work with undamaged and clean seals.
 Do not close aerosol-tight rotors and buckets for storing them.

Instructions for use

2

S-4-72/S-4-104
English (EN)

3 Centrifuging

Follow the notes in the operating manual of the centrifuge on inserting the rotor,
symmetric loading of buckets, max. load per bucket and the max. loading height/
tube length of the buckets.

3.1 Rotor S-4-104 only

NOTICE! Buckets swinging out in the wrong direction.

When working with the rotor S-4-104, using the wrong adapters with 500 mL
Corning tubes may lead to the loss of samples or damage to the centrifuge.

 Only use the Eppendorf adapter intended for this purpose.

Inserting and removing the rotor S-4-104

 To insert and remove the rotor S-4-104, use the rotor key supplied with the rotor. This

rotor key is different from the one in the delivery package of Centrifuge 5810/5810 R.

Loading 750-mL round buckets
To minimize imbalance align the adapters in the 750-mL round bucket symmetrically as

follows.

 Turn the adapter in the 750-mL round bucket to the position where the markings on

the adapter and the buckets are aligned.

Closing the bucket with the cap

NOTICE! Damage to the cap hook.

If the cap is not fitted correctly on the bucket the clamp may break during
closing.

 Before you fold the clamp, check that the cap is positioned correctly.