Instructions for use Eppendorf Rotorsseeon p.Fig.Tab.p.
English (EN)Instructions for use
Instructions for use
Eppendorf Rotors
English (EN)
These instructions for use for rotors supplement the operating manual for the centrifuge
and do not replace it. Therefore, please also read the operating manual before starting up
the rotors for the first time. You can find the current version of the operating manual on
the Internet at
www.eppendorf.com
1 Safety notes
CAUTION! Risk of injury from improperly attached rotors, rotor lids and caps.
Only centrifuge with firmly tightened rotor and rotor lid as well as with
inserted carriers, buckets and correctly closed caps.
If unusual noises occur when the centrifuge starts, the rotor, the rotor lid or a
cap may not be properly secured. Stop centrifugation immediately by
pressing the start/stop.
CAUTION! Risk of injury due to asymmetric loading of a rotor.
Load rotors symmetrically with identical tubes and/or buckets and plates.
Only load adapters with the suitable tubes and plates.
Always use the same type of tubes and plates (weight, material/density and
volume). Always observe the max. g-force of the tubes and plates as indicated
by the manufacturer.
Check that loading is symmetrical by balancing the adapters and tubes and/or
plates used with scales.
CAUTION! Risk of injury from overloaded rotor.
The centrifuges are designed for the centrifugation of material with a max.
density of 1.2 g/mL at maximum speed and with maximum filling volume resp.
load.
Please note the information on each rotor on the maximum load (adapter,
tube and contents) per rotor bore and/or per bucket and do not exceed it.
NOTICE! Damage to rotors from aggressive chemicals.
Rotors are high-quality components which withstand extreme stresses. This
stability can be impaired by aggressive chemicals.
Avoid the use of aggressive chemicals, including strong and weak alkali,
strong acids, solutions with mercury, copper and other heavy metal ions,
halogenated hydrocarbons, concentrated saline solutions and phenol.
If the rotor is contaminated with aggressive chemicals, immediately clean it
using a neutral cleaning agent and then rinse it thoroughly with water. This
applies to the rotor bores in particular.
Due to the manufacturing process, color variations may occur on rotors
marked "coated". These color variations do not effect service life or resistance
to chemicals.
Instructions for use
2
Eppendorf Rotors
English (EN)
CAUTION! Risk of injury due to chemically damaged rotor lids or caps.
Transparent rotor lids or caps made from PC, PP or PEI may lose their strength
under the impact of organic solvents (e.g. phenol, chloroform).
If rotor lids or caps have come into contact with organic solvents, they should
be cleaned immediately.
Regularly check the rotor lids and caps for damages and cracks.
Replace rotor lids or caps which show cracks or milky stains immediately.
NOTICE! If handled incorrectly, the swing-bucket rotor can fall over.
Buckets from swing-bucket rotors may not be used as handles.
Before moving the rotor, remove the buckets.
Always grip the rotor on the rotor cross using both hands.
NOTICE! If handled incorrectly, the rotor can fall over.
Always pick up the rotor F-35-48-17 with both hands.
In order to hold the rotor safely, possibly you have to remove 3 to 4 sleeves
from the opposite outer row.
CAUTION! Risk of injury when turning the rotor manually.
For A-2-MTP rotors, in particular, please take care not to pinch your fingers
and not to get caught on the swinging buckets.
Instructions for use
Eppendorf Rotors
English (EN)
2 Service life
CAUTION! Risk of injury from chemically or mechanically damaged
accessories.
Even minor scratches and cracks can result in serious damage to the
accessories.
Protect all accessory parts from damage.
Inspect the accessories for damage before each use. Replace any damaged
accessories.
Do not use any rotors, rotor lids, buckets or caps showing signs of corrosion
or mechanical damage (e.g. bending).
Do not use any accessories which are past their use-by date.
When inserting the buckets and rotors, ensure that they do not become
scratched.
Since 2012, Eppendorf has been stating the maximum service life of the rotors and
accessories both in years and in the maximum number of cycles. The decisive factor for
the service life is which case occurs first, usually this is the number of years in operation.
Rotor/accessories Centrifuge Max. service life from initial start-up
in cycles in years
A-2-DWP-AT 5810/5810 R 100 000 7
A-2-DWP 5804/5804 R/5810/
5810 R
A-2-MTP 5430/5430 R 100 000 7
A-4-38 5702/5702 R/
5702 RH
A-4-44 5804/5804 R/5810/
5810 R
A-4-62 5810/5810 R40 000 7
A-4-81 5810/5810 R 100 000 7
A-8-17 5702/5702 R/
5702 RH
F-34-6-38 5804/5804 R/5810/
5810 R
F-35-48-17 5804/5804 R/5810/
5810 R
FA-45-6-30 5804/5804 R/5810/
5810 R
34 000 7
100 000 7
34 000 7
75 000 7
75 000 7
100 000 7
100 000 7
3
Instructions for use
4
Eppendorf Rotors
English (EN)
Rotor/accessories Centrifuge Max. service life from initial start-up
in cycles in years
FA-45-48-11 5427 R/5430/5430 R
5804/5804 R/5810/
5810 R
FA-45-16-17 5430/5430 R 100 000 7
FA-45-12-17 5427 R 100 000 7
FA-45-20-17 5804/5804 R/5810/
5810 R
S-4-72 5804/5804 R/5810/
5810 R
S-4-104 5810/5810 R 100 000 7
S-24-11-AT 5427 R/5430/5430 R 100 000 7
T-60-11 5804/5804 R/5810/
5810 R
QuickLock rotor lid – – 3
Rotor lid and caps made
from polycarbonate (PC),
polypropylene (PP) or
polyetherimide (PEI)
A-8-11 bucket 5417 R–3
Plastic adapters – – 1
––3
100 000 7
100 000 7
100 000 7
n/a 7
For all other rotors and their rotor lids there is no service life limit as long as the following
conditions are met:
• correct use
• recommended care
• undamaged condition
On rotors, the manufacturing date is embossed in the format 03/12 (= March 2012) and/or
the inside of the plastic lids, in the shape of a clock .
To ensure aerosol tightness, the following applies:
Exchange aerosol-tight rotor lids and caps after 50 autoclaving cycles.
For QuickLock rotor lids, replace the seal after 50 autoclaving cycles.
Instructions for use
Eppendorf Rotors
English (EN)
3 Cleaning and disinfection
Use a mild cleaning agent for cleaning. For disinfection, use alcohol (ethanol,
isopropanol) or cleaning agents which contain alcohol.
Clean and disinfect the rotor and all accessories (rotor lid, buckets, sleeves, adapters,
buckets and removal support).
1. Remove the rotor from the centrifuge.
2. Use a test tube brush to clean/disinfect the rotor bores on fixed-angle rotors or sleeves.
Do not immerse the rotor as liquid may penetrate the openings.
3. QuickLock rotor lids and caps: Remove the seals and clean the groove below.
4. Clean the rotor cone with a soft, dry and lint-free cloth. Do not lubricate the rotor cone.
5. Check the rotor cone for damage.
6. Place the rotors and accessories on a cloth to dry. Place fixed-angle rotors with the
rotor bores facing downwards.
7. QuickLock rotor lids and caps: Before the next centrifugation, replace the seal
correctly into the clean and dry groove.
With the exception of rotor crosses A-2-MTP, A-4-81, S-4-72 and, S-4-104 all
rotors, rotor lids, buckets, caps and adapters can be autoclaved (121 °C, 20 min).
After a maximum of 50 autoclaving cycles, the caps and, for QuickLock rotors,
the seals must be replaced.
Do not use any stained, porose or otherwise defective gaskets. Also note the
operating manual of the centrifuge and the supplement sheet on aerosol-tight
centrifugation delivered together with the aerosol-tight rotors.
After each cleaning and autoclaving process (121 °C, 20 min) and if it does
not swing freely, apply a thin layer of pivot grease (order no. International
5810 350.050/North America 022634330) to the bucket grooves.
Regularly apply a thin layer of pivot grease to the lid thread on fixed-angle
rotors to prevent any damage.
Ensure that the rotor cross pivots and the bucket grooves are free from
contamination.
QuickLock® is a registered trademark of Eppendorf AG.
Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
©2013 · Eppendorf
5804 700.961-11/082013
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