How it Works
Eppendorf
Loading...
Research pro
User Manual
60 pgs2.83 Mb1
User Manual [en, de, it]
64 pgs2.48 Mb0
Table of contents
Loading...

Eppendorf Research pro User Manual

®
Eppendorf Research pro
Instruction Manual · Mode d’emploi · Manual de Instrucciones
Inhalt / Contents / Sommaire / Indice / Indice
Teil A / Part A / Section A / Parte A / Parte A
Manual de Instrucciones ............................................................................................................63
Part B / Teil B
Programming / Programas .........................................................................................................93
Ordering Information / Programa de ventas............................................................................. 109
Contents / Sommaire / Indice

Evaluate the quality of our operating manuals on www.eppendorf.com/manualfeedback

Evaluer nos manuels d'utilisation sur www.eppendorf.fr/manualfeedback!
¡Díganos que opina de nuestras instrucciones de manejo en www.eppendorf.es/manualfeedback!
eppendorf and eppendorf Research are registered trademarks. Registered trademarks are not marked in all cases with
Research pro pipettes are manufactured under U.S. Patent No. 4,671,123; 4,905,526; 5,187,990; 6,199,435; 6,499,365
No part of this publication may be reproduced without the prior permission of the copyright owner Copyright
©
2000 Eppendorf AG, Hamburg
TM
or ® in this manual.
2

Part A – Contents

This operating manual is valid as of software version V.1.56 and data record 2. Software version and data record appear in the display after the battery pack has been inserted (see Sec. 3.3).
1 Safety precautions and applicational limitations..................................................... 4
Intended use ................................................................................................................. 4
Handling ........................................................................................................................4
Care and maintenance.................................................................................................. 4
Battery........................................................................................................................... 4
2 Technical data.............................................................................................................. 5
Battery........................................................................................................................... 5
Power unit ..................................................................................................................... 5
3 Startup ......................................................................................................................... 6
3.1 Delivery package........................................................................................................... 6
3.2 Inserting the battery ...................................................................................................... 6
4 Operating principle ..................................................................................................... 7
4.1 Display and keypad....................................................................................................... 7
4.2 Function units................................................................................................................ 8
Ejector with extension................................................................................................... 8
Hook on the hand rest................................................................................................... 8
Contact surface on the rear of the pipette.................................................................... 8
Acoustic signals ............................................................................................................ 8
5 Operation ..................................................................................................................... 6
5.1 Mode of operation......................................................................................................... 9
5.2 Essential operating information..................................................................................... 9
Aspirating and dispensing speeds.............................................................................. 10
Loading gels................................................................................................................ 10
5.3 Pipetting in the standard mode................................................................................... 11
5.4 Pipetting with separate blow-out (Blow = BLO).......................................................... 12
5.5 Pipetting with separate rinsing (Rinse = RNS) ............................................................ 13
5.6 Reverse Pipetting (RP) ................................................................................................ 14
5.7 Pipetting with the MAN option .................................................................................... 15
5.8 Pipetting with a preselected fixed volume (FIX) .......................................................... 17
5.10 Notes on the programs ...............................................................................................21
5.10.1 Example for programming pipetting in the program level........................................... 23
5.11 Device parameters ...................................................................................................... 24
5.11.1 Changing the calibration (CAL) ................................................................................... 25
5.11.2 Information on initialization (INI).................................................................................. 26
6 Care, sterilization and maintenance........................................................................ 27
6.1 Care............................................................................................................................. 27
Lower part of multi-channel model ............................................................................. 27
Lower part of single-channel model............................................................................ 28
6.4 Decontamination prior to dispatch.............................................................................. 29
7 Troubleshooting ........................................................................................................30
Program notes only for program version prior to V. 1.56 ............................................ 32
A
Contents
3

1 Safety precautions and applicational limitations

1

Intended use

The Research pro is a lab device intended for dispensing liquids in the volume range from
0.5 µL to 5 mL, in combination with matching pipette tips. In vivo applications (applications in or on the human body) are not permitted. The Research pro may only be operated by trained specialist staff. All users must have read the operating manual carefully and familiarized themselves with the device's mode of operation.
To guarantee problem-free, safe operation of the pipette, it is essential to observe the following points:

Handling

– Use the Research pro only when a pipette tip is attached. – Pipette tips are solely designed for disposable usage. – Do not lay down the Research pro when a filled pipette tip is attached. – After liquid has been aspirated, press the Actuate key only when you are sure that the liquid
shall be dispensed correctly.
– When the pipette is switched on and when the Reset key is pressed, the piston may move.
Please ensure beforehand that the liquid in the pipette tip shall be dispensed correctly. – Emergency stop: A moving piston can be stopped by pressing the Reset key. – When using infectious, radioactive, toxic and other solutions which may pose health risks,
please observe the safety precautions laid down for your country. – Do not use the Research pro in a potentially explosive environment or with potentially
explosive chemicals. – When using organic solvents and aggressive chemicals or their vapors, please check their
Safety precautions and applicational limitations
compatibility with the pipette tip (PP) and pipette. – When using solutions with physical data which deviates greatly from those of water, carry
out the procedure for checking the dispensing volume, as described in Sec. 5.11.1. – Operate the Research pro at temperatures between 15 °C and 40 °C only and at a humidity
of max. 80 % at a temperature up to 31 °C. – When using Filtertips or 200 µL tips with the 300 µL pipette, observe the volume restriction
(see Sec. 5.11).

Care and maintenance

– Do not clean the display or any of the labeling using acetone or aggressive solutions. – Do not allow any liquid to enter the device. – Repairs may be carried out by authorized service personnel only. – Use original spare parts and accessories (battery, pipette tips) only.

Battery

– Charge up the battery before operating the device for the first time (see Sec 3). – Charge the battery in the pipette only, using the charging adapter or the charging stand and
the original power unit. – If a flashing battery symbol appears in the display, stop dispensing and charge the battery. – When changing the battery, do not allow the battery to come into contact with any metallic
surfaces. – Dispose of used batteries as special waste, in accordance with legal stipulations to this
effect. Batteries may not be disposed of together with household waste! – If rechargeable batteries are damaged, avoid touching them with the hands. – Never charge the Research pro without a battery. – Before storing the Research pro for a long period, be sure to remove the charged battery. – When the Research pro is stored for a long time, charge the batteries approximately every
4
six months.

2 Technical data

Single-channel
Volume range Step size Color Pipette tip Volume Max. permissible error
code epT.I.P.S. Error
systematic random
µL µL µL % µL % µL
0.5 – 10 0.01
5 – 100 0.1
20 – 300 0.5 orange 300 µL 30
50 – 1,000 1 blue 1,000 µL 100
100 – 5,000 10 violet 5 mL 500
Multi-channel
0.5 – 10 0.01
5 – 100 0.1
20 – 300 0.5 orange 300 µL 30
50 – 1,200 5 green 1,250 µL 120
The technical data is valid only when the Research pro is used with Eppendorf pipette tips. The multi-channel data is valid for eight- and twelve-channel pipettes. The 50 – 1,200 model is available as an eight-channel version only.
Test conditions in accordance with ISO 8655 for piston-stroke pipettes with an air cushion by means of a standardized fine balance with a moisture trap. Number of determinations: 10 pipettings; degassed distilled water, 20 °C – 25 °C ± 0.5 °C; maximum speed; PIP in standard mode of operation (Sec. 5.3); prewetted pipette tip; dispensing onto inside of tube. If the place where the pipette is used is at extremely high altitude, an adjustment must be made in line with the ambient air pressure.

Battery

Nickel-metal hydride battery pack, 1,200 mAh / 2.4 V reversible overcurrent and over­temperature protection. Overcharging protection when connected to Research pro. Charging time: approx. 9.5 hours for a fully discharged battery.

Power unit

Input voltage, country-specific: 230 V ± 10 %, 50/60 Hz; 120 V ± 10 %, 60 Hz; 100 V ± 10 %, 50/60 Hz; 240 V ± 10 %, 50 Hz Output voltage: 9 V DC; 200 mA (1.8 VA)
Technical specifications subject to change!
light gray
yellow
light gray
yellow 200 µL 10
20 µL L 1
10
200 µL 10
50
100
150 300
500
1,000
2,500 5,000
20 µL L 1
10
50
100
150 300
600
1,200
± 0.018
± 2.5 ± 1.5
5
± 1.0 ± 2.0
± 1.0 ± 0.8 ± 2.5 ± 1.0 ± 0.6 ± 3.0 ± 1.0 ± 0.6 ± 3.0 ± 1.2 ± 0.6
± 5.0 ± 3.0
5
± 2.0 ± 2.0
± 1.0 ± 0.8 ± 2.5 ± 1.0 ± 0.6 ± 6.0 ± 2.7 ± 1.2
± 0.025 ± 0.075 ± 0.1
± 0.2 ± 0.5 ± 0.8 ± 0.75 ± 1.5
± 1.8 ± 3 ± 5 ± 6 ± 15 ± 30 ± 30
± 0.05
± 0.15
± 0.2
± 0.2
± 0.5
± 0.8
± 0.75
± 1.5
± 1.8
± 7.2 ± 16.2 ± 14.4
± 1.8 ± 0.8 ± 0.4
± 1.0 ± 0.3 ± 0.2 ± 0.7 ± 0.3 ± 0.2 ± 0.6 ± 0.2 ± 0.2 ± 0.6 ± 0.25 ± 0.15
± 3.0 ± 1.5
± 0.8 ± 2.0
± 0.8 ± 0.25 ± 1.0
± 0.5
± 0.25
± 0.9
± 0.4
± 0.3
± 0.04 ± 0.04
± 0.1 ± 0.15 ± 0.2 ± 0.21 ± 0.45 ± 0.6 ± 0.6 ± 1 ± 2 ± 3 ± 6.25 ± 7.5
± 0.03 ± 0.075 ± 0.08
± 0.2 ± 0.4 ± 0.25 ± 0.3 ± 0.75 ± 0.75 ± 1.08 ± 2.4 ± 3.6
2
Technical data
5

3 Startup

3
Startup

3.1 Delivery package

The Research pro is supplied as a single-channel, eight-channel or twelve-channel pipette for different volume ranges. The pipette is delivered with or without a charging adapter, depending on the order number. If the pipette is ordered without an adapter, a charging stand for one or four pipettes is required. If this charging stand is not already available in the lab, it must be ordered separately. According to the type of order, the delivery package contains the following:
• Charging adapter with connected power unit or, if necessary, charging stand with separate
power unit
• Special tool(s) according to the pipette type
• Operating manual with measuring protocol and EC conformity assurance document
• Ni-MH battery pack
• Tube of silicone grease
• For multi-channel pipettes: Reagent reservoir for liquid aspiration

3.2 Inserting the battery

Using your thumb and forefinger, take hold of the lid of the battery
NiMH 2.4V / 1200 mAh
XXXXX
50–1000 µL
Program
Speed
record). The battery must be fully charged before the device is used for the first time.

3.3 Charging the battery

compartment on the rear of the pipette and remove it by tilting it to the side
4860 501.002-xx
and pulling it upwards. Insert the battery pack (see diagram). Close the lid of the battery compartment. If the battery has been inserted incorrectly, the lid cannot be closed properly. The positive and negative poles of the battery should not come into contact with any metallic objects outside the pipette. When the battery is inserted, the Research pro carries
Option
out a test routine (e.g. for the display of pipette type, software version, data
The battery is charged in the pipette. Charging may only be carried out using the charging adapter supplied or the charging stand with the original power unit. A pipette which does not contain a battery must not be recharged! A new battery does not attain its full capacity until two or three complete charging/discharging cycles have been completed.
Before recharging, please compare your voltage requirements with the voltage specifications on the plug-in unit. For charging purposes, the charging adapter is placed upon the Research pro. Alternatively, the Research pro can be placed in the charging stand. During the start and termination of the charging process, "CON" appears briefly on the left-hand side of the display. The display is
switched on during the charging process. The battery symbol appears in a rolling form. During charging, the Dispensing function is frozen. When the charging process has finished, the battery symbol appears in the display without moving. When the pipette has been charged, it may remain in the charging stand. To increase the charging capacity, the battery should occasionally be discharged until the flashing battery symbol appears. To avoid a total discharge, the fully charged battery should be removed from the Research pro before extended periods of non-use (e.g. several months). In this case, all data remains stored. In order to avoid a total discharge, the battery that has been removed should be re-charged every six months. Dispose of used batteries according to legal regulations.
6

4 Operating principle

4.1 Display and keypad

Aspirating / dispensing
Flashes when the battery is low
Appears during
Appears at the
program level
Double-click to open the
program level.
PROG 1 – 5 appears in the
Speed, option, volume are
frozen. The program level
can be quit by pressing the
Display of speeds
and
by pressing
key repeatedly.
Change by pressing .
This switch can be pressed
upwards or downwards.
Battery symbol
editing of
programs
Number of
the program
Program
display.
key once.
Speed
Select
Select rocker
Selection of basic modes:
PIP = Pipetting (Sec. 5.1 – 5.7) DIS = Dispensing (Sec. 5.9) FIX = Fixed-volume
for pipetting (Sec. 5.8)
After double-click : Selection in the program level
Program
(Sec. 5.10)
Direction
EDIT
Program
Select
Ejector
for pipette tips
50–1000 µL
1PROG 2345
Speed
R
Speed
R
Volum
Actuate key
for aspirating and dispensing liquid.
ENTER key with .
To switch on: Press any key firmly To switch off: Automatically after 10 min of non-use
Option
CAL
e
EDIT
OptionsOperating mode
Appears when factory calibration is replaced by user calibration.
Appears when Reset is requested.
RESET key
Liquid can be dispensed during a Reset! When the piston is moving: Emergency stop!
Option
For PIP and FIX, the options are selected by pressing the key repeatedly:
= Display blank: Standard
operation (Sec. 5.3)
BLO = BLOW (separate blow-
out, Sec. 5.4)
RNS = RINSE (Rinsing /
mixing, Sec. 5.5)
RP =REVERSE PIPETTING
(Sec. 5.6)
MAN =Manual (Sec. 5.7)
Volume rocker
for changing the volume. For FIX: To select the fixed volume.
The rocker can be pressed upwards or downwards.
Pressed briefly: Individual
Held down: Rapid changes 2nd pressure point: High speed
Automatic stop for min./max. volume and for overflow obtained by releasing and then pressing the rocker again.
changes
4
Operating principle
7
4 Operating principle
4

4.2 Function units

The Research pro is a microprocessor-controlled pipette which executes the piston movement with the aid of a stepper motor. The power supply is a rechargeable nickel-metal hydride battery.
Upper end position
Basic position
End of blow-out
Operating principle
Dispensing and Reverse Pipetting require a different basic position than that for Pipetting. The request to change the basic position is indicated in the display by .
Ejector with extension
Ejector
Extension
Hook on the hand rest
Loosening the screw enables the hook on the rear of the pipette to be adjusted to suit all hand sizes.
Contact surface on the rear of the pipette
Both upper surfaces are required for charging the nickel-metal hydride battery. The lower surfaces are the data interfaces for the Service Department. The contact to the charging adapter or the charging stand cannot be confused with any other contacts.
Acoustic signals
Certain signals assist the user for different operations:
– Short signal to acknowledge that the keypad has been pressed. – Slightly higher tone to indicate that the function for which the key has been pressed cannot
be executed. – Louder tone to indicate that a piston movement has ended. – Longer tone to indicate that a specific sequence of steps (e.g. Dispensing) has ended. – Louder tone to indicate an error message.
The acoustic signals are a great help when you are familiarizing yourself with the operating procedure of the Research pro. They can also be switched off if required (see Sec. 5.11).
8
For sample aspiration, the piston moves upwards from the basic position. The sample is aspirated into the pipette tip.
Return to the basic position – via short single steps
= Dispensing
– entire path = Pipetting.
Residual liquid discarded via blow-out (BLOW).
R
The force translation of the ejector for pipette tips ensures that tips are ejected very easily. The extension can be individually adjusted to accommodate left­and right-handed users as well as different hand sizes. Select the desired setting by simply sliding the extension into the position as required.

5 Operation

5.1 Mode of operation

The Research pro is switched on by pressing any key. Attach an Eppendorf pipette tip to the Research pro. The color coding of the Actuate key corresponds to the color code of the racks for pipette tips. The dispensing liquid is aspirated into the pipette tip.
When the 200 µL tips are used together with the 300 µL pipette and when Filtertips are used, the volume restriction option must be activated in the device parameters (Sec. 5.11).
The liquid which is to be aspirated is taken from a suitable vessel. When multi-channel pipettes are used, the "Reagent reservoir" vessel is recommended. Before commencing pipetting activities with multi-channel pipettes, turn the adapter in the required direction. In addition to the description found in the subsequent sections
5.3 – 5.11, the following general procedure takes place: Attach the pipette from the rack. Slight force may be used if necessary.
When aspirating liquid, immerse the pipette vertically – and as little as possible – into the liquid. Following aspiration, remove the pipette tip from the liquid after the acoustic signal has been emitted. If air bubbles have been aspirated, this process must be repeated.
Never lay the pipette down when the tip is filled!
If necessary, carefully remove any external wetting from the pipette tip. To dispense liquid, position the pipette tip in the aspirating vessel, as shown in the illustration. Depending on the type of problem (carryover, contamination), discard the pipette tip after that by pressing the Eject key, attach a new tip and reaspirate liquid.
5
Operation

5.2 Essential operating information

The following sections contain step-by-step explanations of the operating procedure. It is essential to work through these sections with the pipette in your hand. The volume shown in the display information contained in the operating manual does not necessarily correspond to the volume range of your pipette. As a supplement to this manual, an interactive demonstration is available to familiarize users with the operation of the Research pro – see our home page www.eppendorfna.com
Reset
If a Reset is requested in the display, this Reset is always accompanied by a piston movement. For this reason, please ensure that any residual liquid in the pipette tip is
R
dispensed beforehand! A Reset can also be used to empty the pipette tip and to end an operating process. If you quit an input field during programming (e.g. changing the speed) using Reset, the changes which have been made are not stored.
R
If is pressed during the piston movement, this functions as an emergency brake. Pressing again empties the pipette tip.
R
9
Program
Speed
Speed
5 Operation
5
Operation
Motor­reset
R
If is held down until a piston movement begins, a motor reset is effected. This reset routine lasts roughly 10 seconds. The accuracy of the piston movement is ascertained with the motor reset.
The Research pro has two operating levels. The basic modes for pipetting (PIP and FIX) and dispensing (DIS) are accessed in the first level by pressing the Select rocker. PROG appears in the display by double-clicking the "Program" key. You are now in the second level (program level). Using the Select rocker, select from the programs (Sec. 5.10). This level can be quit by pressing the "Program" key briefly once. PROG disappears from the display.
Please note the following descriptions of the symbols used in the program sequence:
An Actuate key which has been pressed briefly is light.
An Actuate key which has been held down is dark.
If the piston movements appear in the display without any volumes, this indicates a process which is not connected to liquid aspiration or dispensing.
Aspirating and dispensing speeds
The selected speed can be viewed by pressing the Speed key several times. The speed is changed as follows:
Sequence
for pressing keys
Select
Magnifying glass indicates important display information
Comment
Display and selection of aspirating speed.
Select
Speed
*
Display and selection of dispensing speed.
* This procedure does not necessarily have to be completed using the Speed key. Any key –
with the exception of Reset – may be used. If a key other than the Speed key is used to complete the procedure, the function of this key is also executed at the same time.
The direction of the arrow for speed flashes in this input field. The speed can be altered before every dispensing or aspiration. Different speeds can already be stored for the three basic modes (PIP, DIS, FIX).
Important! High speeds have a much lower current consumption than low speeds.
Loading gels
With the 10 µL and 100 µL Research pro, the lowest speeds for liquid dispensing are ideal for loading gels. To enable users to work rapidly and with a low electricity consumption, the highest possible speed for liquid aspirating should be used. Depending on the task at hand, gels can be loaded in the Standard mode (Sec. 5.3) or by using the BLO (Sec. 5.4) or MAN (Sec. 5.7) options. Using the BLO option makes it possible to discharge the blowout outside of the gel pouch, thus preventing turbulence in the gel pouch.
10
5 Operation

5.3 Pipetting in the standard mode

In the Standard mode, blow-out is executed automatically when liquid is dispensed. The standard mode is recommended for rapid series pipetting with aqueous solutions.
Programming
Select
If PROG appears in the display: Press
Program
the key
Activate the selection rocker until "PIP" appears in the left side of the display
.
briefly until PROG disappears.
Vol ume
Volume selection (here: 800 µL). The volume rocker has two pressure points for fast or slow volume adjustment for up and down.
Option
If necessary, press the Option key
Option
Option
several times. In the Standard mode, the display remains blank in this case.
Pipetting procedure
R
If appears in the display: Press the key briefly.
R
Liquid is aspirated.
5
Operation
Alternative pipetting procedure
Liquid is dispensed with blow-out.
If the Actuate key is held down when liquid is dispensed, the device returns to the basic
➞
position when the key is released. Note: Necessary when dispensing into existing liquid: Before releasing the key, remove the pipette tip from the solution in order to prevent any solution from being aspirated unintentionally.
The pipette is now once again ready to aspirate.
11
5 Operation
5
Operation

5.4 Pipetting with separate blow-out (Blow = BLO)

BLO is recommended for use with liquids with a high wetting power or with liquids which are prone to the formation of foam (e.g. solutions containing protein). The residual liquid is dispensed by pressing the Actuate key separately.
Programming
Select
If PROG appears in the display: Press
Program
the key
Select "PIP" for pipetting.
briefly until PROG disappears.
Vol ume
Volume selection (here: 800 µL). The volume rocker has two pressure points for fast or slow volume adjustment for up and down.
Option
If necessary, press the Option
Option
Option
key several times until BLO appears in the display. BLO is selected for blow-out.
Procedure
R
If appears in the display: Press the key briefly.
R
Liquid is aspirated.
12
Alternative blow-out procedure
Liquid is dispensed.
Blow-out: Delayed dispensing of residual liquid.
If the Actuate key is held down during the blow-out, the return does not take place
➞
until the key has been released.
The pipette is now once again ready to aspirate.
5 Operation

5.5 Pipetting with separate rinsing (Rinse = RNS)

Rinsing (RNS) is recommended for volumes below 10 µL. RNS is suitable for mixing the dispensing volume and the specimen liquid when large volumes are used. It is strongly recommended to use the "max. speed" setting.
Programming
Select
If PROG appears in the display: Press
Program
the key
Select "PIP" for pipetting.
briefly until PROG disappears.
Vol ume
Volume selection (here: 5 µL). The volume rocker has two pressure points for fast or slow volume adjustment for up and down.
Option
If necessary, press the Option
Option
Option
key several times until RNS appears in the display. RNS is selected for rinsing.
Procedure
R
If appears in the display: Press the key briefly.
R
Liquid is aspirated.
Rinsing/mixing takes place three times after liquid has been dispensed. Mixing volume results from dispensing volume and the volume of the blow-out step (BLO).
Alternative mixing procedure
If the Actuate key is held down when liquid is dispensed, mixing takes place until the key is released.
Return to basic position by pressing the key when device is not immersed in the liquid.
5
Operation
The pipette is now once again ready to aspirate.
13
5 Operation
5
Operation

5.6 Reverse Pipetting (RP)

RP is recommended for solutions with a high viscosity or with a slightly higher vapor pressure. "Reverse Pipetting" is liquid aspiration with a blow-out. The liquid is dispensed without a blow­out. If Filtertips are used, please observe the volume restriction (Sec. 5.11).
Programming
Select
If PROG appears in the display: Press
Program
the key
Select "PIP" for pipetting.
briefly until PROG disappears.
Vol ume
Volume selection (here: 800 µL). The volume rocker has two pressure points for fast or slow volume adjustment for up and down.
Option
If necessary, press the Option
Option
Option
key several times, until RP appears in the display. RP is selected.
Procedure
R
If appears in the display: Press the key briefly.
R
Aspiration of liquid with blow­out.
Alternative procedure for dispensing and aspirating
14
Liquid is dispensed.
Blow-out is discarded. If necessary, replace the tip.
Pipette is once again ready to aspirate liquid with blow-out.
If the Actuate key is held down when liquid is dispensed, the next liquid aspiration without blow-out takes place when the key is released. The liquid from the blow-out may continue to be used.
5 Operation

5.7 Pipetting with the MAN option

Note: The MAN (manual) option is the equivalent of operation with a mechanical piston-stroke pipette. Liquid is aspirated and dispensed only when the Actuate key is held down. The volume display increases the number of applications available in comparison to a mechanical pipette:
1. Aspirating: Measuring a small, unknown amount of liquid via aspiration into the pipette tip.
The volume of aspirated liquid appears in the display. If necessary, dilute the liquid to the
defined end volume by aspirating additional liquids. Liquids can be separated by aspirating
air bubbles. The display shows the total volume.
2. Dispensing: Titration is carried out. The amount of liquid dispensed appears in the display.
There is no blow-out. Setting lower speeds is strongly recommended.
Programming
Select
If PROG appears in the display: Press
Program
the key
Select "PIP" for pipetting.
briefly until PROG disappears.
Vol ume
Volume selection (here: 1,000 µL). The volume rocker has two pressure points for fast or slow volume adjustment for up and down.
Option
If necessary, press the Option
Option
Option
key several times, until MAN appears in the display. MAN is selected.
5
Operation
Carrying out liquid aspiration
R
If appears in the display: Press the key briefly.
Vol ume
R
Liquid is aspirated. The aspirated liquid appears in the display.
The direction is changed using the volume rocker.
Volume correction, e.g. for removing an air bubble.
15
or
5 Operation
5
Operation
Vol ume
The direction is changed again using the volume rocker. This change of direction can be repeated without restriction.
A defined end volume has been reached. A special acoustic signal is emitted. Dispensing is carried out by pressing the key again.
Carrying out dispensing/titration
Aspirate the defined total volume by holding down the Actuate key.
When the key is pressed briefly: The amount of liquid dispensed appears in the display.
Press key again: Total of dispensed liquid is displayed.
Note: We recommend low speed for dispensing. The speed can also be reduced between individual dispensing steps (see Ch. 5.2, "Speed for aspiration and discharge").
Total amount of liquid is dispensed. A special acoustic signal is emitted. Blow-out is not executed. Blow-out can be actuated by pressing the key.
Press the key again to aspirate liquid.
R
16
Note:
Vol ume
The direction can also be changed after partial dispensing. The volume display changes after the Actuate key has been pressed. No additional projection of the volume occurs following the repetition of the procedure. The display starts at 0.
Program
Program
5 Operation
5. 8 Pipetting with a preselected fixed volume (FIX)
FIX is used to rapidly set five frequently used pipetting volumes. Five FIX volumes are predefined in the pipette. FIX volumes can be changed via programming.
Programming FIX fixed volumes
Select
If PROG
Select "FIX" for fixed volume.
appears in the display:
Press the
Program
key briefly until PROG disappears.
Hold down the Program key until EDIT appears. The volume flashes.
Select the memory slot using the Select rocker.
Using the Volume rocker, select the volume for the memory slot (here: 5).
Select the next memory slot for purposes of changing the volume.
Select
Vol ume
Select
EDIT
EDIT
3
3
EDIT
12345
EDIT
5
EDIT
12345
5
Operation
Vol ume
EDIT
3
3
or
R
If appears in the display: Press the key briefly.
R
3
Define the next volume (here: memory slot 3).
The program is ended by pressing
Program
the key. EDIT and the flashing volume disappear from the display.
The selected volume is aspirated. Programming is ended. EDIT and the flashing volume disappear from the display.
17
Speed
Speed
5 Operation
5
Operation
Selecting a fixed volume (with option and speed)
Select
If PROG
5
appears in the display:
Press the
Program
key
Select "FIX" for fixed volumes.
briefly until PROG disappears.
Option
Vol ume
Option
Select
3
Option
3
Select from the five FIX volumes.
Press the Option key repeatedly until the desired option appears.
Display and selection of aspirating speed.
Select
Speed
Display and selection of dispensing speed.
Note: The option and speeds selected are applicable for all fixed volumes. It is possible to store the pipetting volume with speed and option by using Prog (Sec. 5.10).
Procedure
The procedure depends on the option selected. Information on the procedure according to the option selected is contained in Sections 5.3 to 5.7.
18
Loading...
+ 42 hidden pages
Buy Points How it Works FAQ Contact Us Questions and Suggestions Privacy Policy Cookie Policy Terms of Use