Eppendorf PiezoXpert User Manual
5194 900.017
Eppendorf PiezoXpert™
Operating manual
Copyright © 2011 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
Trademarks
eppendorf
®
, Eppendorf PiezoXpert™, CellTram®, FemtoJet® InjectMan®, and TransferMan® are
trademarks/registered trademarks of Eppendorf AG, Hamburg, Germany.
3M™ and Fluorinert™ are trademarks of 3M Company, St. Paul, MN, USA
Trademarks are not marked in all cases with ™ or
®
in this manual.
5194 900.017-00/0511
Eppendorf PiezoXpert™ — Operating manual
Operating manual — Eppendorf PiezoXpert™seeon p.Fig.Tab.p.
Operating manual
Table of contents
1 Table of contents
1 User instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Using this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.3 Symbols used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.3 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.1 Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.2 User profile. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.4 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.5 Safety instructions on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.2 Installing the actuator on the micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4.2.1 Installing the actuator on the InjectMan
manufacturers. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
4.2.2 Installing the actuator on the TransferMan
4.3 Mounting the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4 Connecting PiezoXpert and the actuator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5.2 Setting the piezo impulses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.2.1 Setting parameters for piezo impulses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
5.2.2 Saving parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2.3 Accessing saved parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.3 Triggering piezo impulses. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.4 Optimizing parameters for capillaries. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.4.1 Preparing capillaries with larger opening diameters . . . . . . . . . . . . . . . . . . . . 19
5.4.2 Preparing capillaries with smaller opening diameters. . . . . . . . . . . . . . . . . . . 20
5.4.3 Optimizing parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
®
NI 2 or micromanipulators from other
®
NK 2 or the PatchMan NP 2 . . . 12
EN
Operating manual
3
Eppendorf PiezoXpert™ — Operating manual
EN
5.5 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.6 Setting the display contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Operating manual
6.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.1 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
7.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
8.1 Power supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
8.2 Ambient conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
8.3 Weight/dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
8.4 Interfaces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
8.5 Piezo impulse parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
9 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.1 PiezoXpert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
10.1 Storage. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
10.4 Disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.5.1 Accessing the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5.2 Setting the parameters for the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5.3 Saving the parameters for the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . 21
5.5.4 Accessing parameters saved for the Clean function. . . . . . . . . . . . . . . . . . . . 21
5.5.5 Activating the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.1.1 Accessing error codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
9.2.1 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
9.2.2 Microinjection and micromanipulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4
Eppendorf PiezoXpert™ — Operating manual
1 User instructions
1 User instructions
1.1 Using this manual
Please read this operating manual completely before using the device for the first time. Also
observe the instructions for use enclosed with the accessories.
Please view this operating manual as part of the product and keep it somewhere easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our
website www.eppendorf.com
1.2 Danger symbols and danger levels
1.2.1 Danger symbols
Toxic substances Electric shock
Hazard point Material damage
Cuts
1.2.2 Danger levels
The danger level is a part of a safety note and distinguishes the possible results of
non-observance from each other.
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
.
EN
Operating manual
1.3 Symbols used
Depiction Meaning
You are requested to perform an action.
1.
2.
• List.
Text Terms and key names from the software.
Perform these actions in the sequence described.
References useful information.
5
213
5
4
Eppendorf PiezoXpert™ — Operating manual
EN
2 Product description
2 Product description
2.1 Main illustration
Abb. 1:PiezoXpert
4
Operating manual
3
2
1
Fig. 1: PiezoXpert
1 Parameter keys 2 Rotary knobs
3Display 4Program keys
5 clean key 6 Connections
Abb. 2:PiezoXpert Actuator
5
6
Fig. 2: PiezoXpert Actuator
1 Grid head for holding the capillary 2 Front knurled screw
3 Piezo element 4 Connection for pressure tube
5 Connection to PiezoXpert
6
Eppendorf PiezoXpert™ — Operating manual
Abb. 3:Foot control
A
1
Fig. 3: Foot control
1 Left foot control: parameter set A 2 Right foot control: parameter set B
B
2
2.2 Delivery package
Quantity Description
1 PiezoXpert
1Actuator
1 Foot control
1 Power cable
1 Spacer plate (for installation on TransferMan NK 2 and PatchMan NP 2)
1 PiezoXpert operating manual
Store the shipping box and packing material in order to store the device or transport it at
Hint!
a later time.
Check the delivery for completeness.
Check all parts for damage in transit.
EN
Operating manual
2.3 Features
The PiezoXpert is used in micromanipulation. Penetration into cells is supported by piezo
impulses which are directly transmitted to the capillaries loss-free. Intuitive operation and an
additional adjustment range enable reproducible work.
• Optimal transmission of piezo impulses: The piezo element is located at the front of the
capillary holder.
• Loss-free transmission of piezo impulses: The actuator (capillary holder with piezo element)
is rigidly mounted to the micromanipulator.
• Reproducible work: Intensity, speed (frequency) and quantity of piezo impulses can be
simultaneously set in two application-specific sets of parameters. Three memory locations
are available for storing the settings.
• Cleaning function: Parameters for removing contaminations from the capillary can be set and
stored.
• Simple operation: The device can be operated intuitively. piezo impulses can be triggered on
the device or by using the foot control.
7
Eppendorf PiezoXpert™ — Operating manual
EN
3 Safety
3Safety
3.1 Intended use
The PiezoXpert supports micromanipulation and microinjection in research. The PiezoXpert is
intended exclusively for indoor use.
3.2 User profile
This device may only be operated by trained and skilled personnel.
Before using the device, read the operating manual carefully and familiarize yourself with the
device's mode of operation.
Operating manual
3.3 Information on product liability
In the following cases, the designated protection may compromise the safety of the device.
Liability for material damage and personal injury is transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The owner has made unauthorized modifications to the device.
3.4 Warnings for intended use
WARNING!
WARNING!
WARNING! Damage to health from toxic, radioactive or aggressive chemicals as
well as infectious liquids and pathogenic germs.
Observe the national regulations for handling these substances, the biological
security level of your laboratory, the material safety data sheets and the
manufacturer's application notes.
Wear personal protective equipment (PPE).
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside
the device can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have
been specifically authorized by Eppendorf.
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
WARNING!
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply.
WARNING! Risk from incorrect supply voltage
Only connect the device to power supplies which correspond with the electrical
WARNING!
requirements on the nameplate.
Only use sockets with a protective earth (PE) conductor and a suitable mains cable.
8
Eppendorf PiezoXpert™ — Operating manual
WARNING! Risk of injury due to flying capillaries and glass splinters.
If exposed to high pressures, capillaries can detach themselves from the grip heads and
WARNING!
become projectiles.
Capillaries can crack as a result of incorrect handling.
Wear protective goggles.
Never aim capillaries at people.
Use capillaries with an outer diameter that matches the grip head specifications.
Always mount capillaries depressurized.
Mount the capillary correctly in the grip head.
Do not touch the capillary with the Petri dish or other objects.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf
CAUTION!
may impair the safety, function and precision of the device. Eppendorf cannot be held
liable or accept any liability for damage resulting from the use of incorrect or
non-recommended accessories and spare parts or from the improper use of such
equipment.
Only use accessories and original spare parts recommended by Eppendorf.
CAUTION! Risk of cuts when unpacking the capillaries
Capillaries can break as a result of incorrect unpacking.
CAUTION!
Do not reach into the capillary transport protection.
EN
Operating manual
CAUTION! Risk of cuts from broken capillaries.
Capillaries are made of glass and are very fragile.
CAUTION!
Wear personal protective equipment (PPE).
Always mount capillaries depressurized.
Never aim capillaries at people.
Handle the capillaries very carefully.
3.5 Safety instructions on the device
Depiction Meaning Location
Follow the instructions in the
operating manual.
Rear side of the device
9
Eppendorf PiezoXpert™ — Operating manual
EN
4 Installation
4 Installation
4.1 Selecting the location
Please select the location for the PiezoXpert according to the following criteria:
• Mains connection (230 V/120 V/100 V) according to the name plate. This is located on the
rear side of the device.
• At least 10 cm away from adjacent devices and walls.
• Solid bench with stable, horizontal and even work surface.
4.2 Installing the actuator on the micromanipulator
Operating manual
The actuator is mounted on the micromanipulator in the same manner as the capillary holder.
NOTICE!
NOTICE!
Hint!
NOTICE! Damage to the actuator.
Impacts or vibrations can cause the piezo element to malfunction or fail.
Do not allow the actuator to fall.
Do not expose the actuator to strong vibrations.
If you suspect the actuator has been damaged, have it inspected by Eppendorf
Service.
NOTICE! Damage to the piezo element.
The piezo element may become damaged if is twisted.
It may only be rotated on the knurled screws.
Do not use the piezo element as a lever.
When the actuator is mounted for the first time, the micromanipulator must be converted
or realigned as necessary. Follow the instructions in the operating manual of your
micromanipulator.
10
Eppendorf PiezoXpert™ — Operating manual
4.2.1 Installing the actuator on the InjectMan® NI 2 or micromanipulators from other
1. Loosen the knurled screw on the micromanipulator tool holder.
2. Insert the actuator into the support.
3. Tighten the knurled screw.
4. Set an angle between 0° and 25°. The flatter the angle, the more direct the effect of the
manufacturers
piezo impulses.
The PiezoXpert will only optimally transmit the piezo impulses to the capillaries if it is
Hint!
firmly mounted.
• Ensure that the actuator is secured and cannot be moved in the support.
EN
Operating manual
11
Loading...