Eppendorf PCR-Cooler User Manual

pgp

Instructions for use

3881 900.202

PCR-Cooler

Instructions for use

Copyright© 2008 Eppendorf AG, Hamburg. No part of this publication may be
reproduced without the prior permission of the copyright owner.

Trademarks

eppendorf is a registered trademark of Eppendorf AG, Hamburg, Germany.
Registered trademarks are not marked in all cases with ™ or

®

in this manual.

3881 900.202-02/082011

PCR-Cooler — Instructions for use

1 Product description

1 Product description

2 Intended use

2 Intended use

The PCR Cooler is intended exclusively for indoor use and to cool samples during PCR
preparation. Use only Eppendorf accessories or accessories recommended by
Eppendorf.

The PCR Cooler must only be operated by appropriately qualified and trained specialist
personnel. Carefully read the operating manual before working with the PCR cooler and
familiarize yourself with its use.

3Operation

3 Operation

NOTICE! Damage to the PCR-Cooler caused by centrifugation.

The PCR-Cooler is only designed to cool samples during PCR preparation.
Centrifugation forces may result in damage to the PCR-Cooler.

NOTICE!

 Do not centrifuge the PCR-Cooler. Use a refrigerated centrifuge to

centrifuge samples.

1 PCR-Cooler
2 Holder
The PCR-Cooler is a cool pack which is used for

efficient cooling of all standard PCR tubes and

1

plates.
The PCR-Cooler can be used with:

• single 0.2 ml and 0.5 ml tubes

• 0.2 ml strips

• 96-well PCR plates.

2

NOTICE! Damage of the PCR-Cooler from sharp edges.

Sharp edged components can damage the film on the underside of the
PCR-Cooler.

NOTICE!

 Do not use sharp edged components on the PCR-Cooler.

1. Before use, place the PCR-Cooler in a freezer at –20°C for at least 2 hours.
Position the PCR-Cooler so that its bottom side is facing upwards.

2. Allow the PCR-Cooler to stand on the lab bench for approx. 10 minutes.

3

PCR-Cooler — Instructions for use

3. Insert the tubes or the plate.
The tubes or the plate will not freeze. By changing color from violet to pink or from
dark blue to light blue, the PCR-Cooler shows when the upper temperature value of
7°C has been exceeded. It is also possible to read the temperature of single tubes

by the color of the single bore holes.

Abb. 1:Thermal behavior of a precooled PCR-Cooler and a sample inserted at room temperature

Temperatur [°C]

25

22°C

20

15

10

7°C

5

0

–5

–10

–15

–20

Fig. 1: Thermal behavior of a precooled PCR-Cooler and a sample inserted at room

4. The PCR-Cooler can be labeled individually using the cards supplied. These cards

temperature

are inserted into the slots on the sides of the PCR-Cooler.

eingebrachte Probe
(Volumen 0,1 mL)

Kühlaggregat
(PCR-Cooler)

60010–10 120

Zeit [min]

4

PCR-Cooler — Instructions for use

4 Maintenance

4 Maintenance

4.1 Cleaning

Clean the PCR-Cooler and holder immediately if sample fluid enters the bore holes or
comes into contact with the surface.

1. Remove the PCR-Cooler from the holder.

2. Clean with a mild soap solution.

3. Rinse thoroughly with distilled water.

4.2 Disinfection/decontamination

WARNING! Health hazard from contaminated accessories.

1. Follow the instructions in the decontamination certificate. You can find it

WARNING!

 Select a disinfection method complying with the statutory rules and regulations for

your area of application. Use e.g. acohol (ethanol, isopropanol) or
alcohol-containing disinfectants.

as a PDF file on our homepage

decontamination).

2. Decontaminate all the parts you want to dispatch.

3. Include the fully completed decontamination certificate for returned goods
in the package.

5 Ordering Information

5 Ordering Information

5.1 Accessories

(www.eppendorf.com/

Order No.

(International)

3881 000.015
3881 000.023
3881 000.031

Order No.

(North

America)

022510509
022510541
022510525

0030 124.332 951010006

0030 128.575
0030 128.648

951020303
951020401

Description

PCR-Cooler

Starter Set (1 x pink, 1 x blue)
Pink
Blue

PCR Tubes

0.2 mL, colorless, 1,000 pieces

twin.tec PCR Plate 96

Wells colorless
semi-skirted, colorless, 25 pcs.
skirted, colorless, 25 pcs.

5

Eppendorf AG · 22331 Hamburg · Germany · Tel: [phone] · Fax: [phone] · E-mail: [e-mail]

Tel: [phone] · Toll free phone: [phone] · Fax: [phone] · E-mail: [e-mail]

Application Support Europe: Tel: [phone] (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)

Your local distributor: www.eppendorf.com/worldwide

Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA

North America: Tel: [phone] · E-mail: [e-mail]

Asia Pacific: Tel: [phone] · E-mail: [e-mail]

[e-mail]