Eppendorf In situ Adapter User Manual

In situ Adapter

Bedienungsanleitung

Operating Manual

Mode d'emploi

Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Mode d'emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Contents

1 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2 Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3 Function and operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.1 Delivery package and installation . . . . . . . . . . . . . . . . . . . . . . . . 12

3.2 Technical description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.3 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.4 The

4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

5 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

6 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

in situ

reaction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

9

1 General information

The

in situ

Mastercycler® gradient enables
a specially developed carrier system. This allows the Mastercycler® and
the Mastercycler® gradient to be used for the conv entional PCR technique
in different tube systems as well as for the

The
can accommodate up to four glass slides. To ensure optimum temperature
control of the samples, the entire carrier system also has a detachable
covering hood. This covering hood is transparent and double-walled,
which allows access to the sample at all times and also prevents heat
loss.

The adapter is extremely easy to handle: The carrier system is positioned
onto the thermoblock of the Mastercycler
samples are placed on up to four object carriers, fixed into place and
sealed hermetically prior to the PCR. Eppendorf
ideal sealing system for the
(volumes) (see "Ordering information"). The transparent covering hood is
then closed and the PCR may be started.

Thanks to the wide selection of different tube types, the software is
specially tailored to suit the
quality heat transition necessary for optimal results.

Adapter for the Eppendorf Mastercycler® and the Eppendorf

in situ

Adapter is a high-temperature-conductive metal carrier, which

The

in situ

Adapter must be set up and used in accordance
with the operating manual provided in order to protect both the
user and the device. The instructions in the operating manual
of the Mastercycler® or Mastercycler® gradient must also be
observed.

When operating the device, please observe the instructions
contained in the corresponding applicational regulations!

in situ

PCR reactions to be carried out on

in situ

technique.

®

or Mastercycler® gradient. The

in situ

in situ

PCR and are availab le in diff erent siz es

in situ

Adapter, which guarantees the high-

Frames form the

10

2 Safety precautions

Voltage

Before startup, please ensure that your power supply
corresponds to the voltage specifications on the identification
plate!
Mastercycler or Mastercycler gradient must be plugged into
a grounded socket.
Before carrying out maintenance or cleaning work or
replacing the fuses, switch off the Mastercycler,
Mastercycler gradient or
from the mains supply.

Explosive substances

Do not use explosive or highly reactive substances in the
Mastercycler® or Mastercycler® gradient or in combination
with the

in situ

Adapter! Do not operate the Mastercycler®,
Mastercycler® gradient or the
explosive environment!

Liquids

Do not allow any liquid to enter into the device!

Danger of burns

The thermoblock,
the micro test tubes and microtiter plates attain a temperature
in excess of 50 °C extremely rapidly.

Close the covering hood of the
after starting the PCR. Ensure that the covering hood remains
closed until the temperature is 30 °C or lower.

Repairs may be carried out by trained specialists only. Only use original
Eppendorf spare parts. Disconnect the mains supply before opening the
Mastercycler® or Mastercycler® gradient.

Eppendorf AG reserves the right to make technical modifications to this
product.

This operating manual may be reproduced in part or in full only with the
prior written permission of Eppendorf AG.

in situ

adapter and disconnect them

in situ

Adapter in a potentially

in situ

Adapter, inside of the heated lid and

in situ

Adapter immediately

11

3 Function and operation

3.1 Delivery package and installation

Delivery package

– Metal carrier
–Transparent covering hood (double-walled)
–Transparent plate attachment
– Operating manual

Together with a transparent plate attachment, the covering hood offers
protection during transport. The plate attachment and the covering hood
should also be used for storing the adapter.

When an
inserted into the 0.5 ml positions of the thermoblock. There is no
privileged direction (left/right).

Caution:

Mastercycler® gradient!

in situ

PCR is carried out, the "pegs" on the underside are

Only a

cold

adapter may be attached to the Mastercycler® and

Mastercycler gradient

12

in situ

Adapter

3.2 Technical description

The glass slides are placed onto the four contact surfaces of the metal
carrier:

Up to four glass slides can be attached to the carrier

3.3 Programming

The Mastercycler / Mastercycler gradient is programmed in accordance
with the operating manual for these devices. The
by the Mastercycler and Mastercycler gradient like all other tube types

in situ

PCR,

"in situ"

(i.e. for the

is selected instead of the tube types
"0.2 ml", "0.5 ml" and "plate"). Specific settings for the adapter are thus
selected.

During such programming, please observe that the temperature of the
sample (TUBE) is regulated. The lid heating
(corresponds to LID=0°), irrespective of the entry in the LID field.

in situ

Adapter is treated

switches off automatically

and the temperature control of the lid switches off

FILES... Edit UNNAMED
Edit CNTRL TUBE
Load LID=0°
Standard NOWAIT AUTO
New 1 T=94.0° 00:02:00
Delete 2 T=94.0° 00:00:15
UNNAMED 3 T=44.0° 00:00:15
– –/22.0° 10:22:06

Temperature regulation is set to TUBE

13

For Mastercycler gradient only: The Gradient function switches off
automatically, independent of the entry in the Gradient field:

The gradient is switched off

FILES... Edit UNNAMED
Edit 3 T=44.0° 0:00:15
Load 4 T=72.0° 0:00:30
Standard +0.0° +0:01
New R= 3.0°/s +0.1°/s
Delete G=0.0°
UNNAMED 5 GOTO2 REP24
– –/22.0° 10:22:06

3.4 The in situ reaction

– Place the
– Prepare the glass slides (sample, reagents and sealing method) and

place them onto the metal carriers.

– After preparing the glass slides, the transparent covering hood is closed

over this arrangement of thermoblock and metal carrier. The lid of the
Mastercycler

– The Mastercycler

manner. The desired
program must contain the CNTRL-TUBE command, the tube type

"in situ"

the program should be started by pressing :

in situ

Adapter onto the thermoblock.



or Mastercycler gradient remains open!



or Mastercycler gradient is started up in the usual

in situ

program is then selected. As an

in situ

should be selected in the following menu using the key and

Select the

Main-Menu Run a Program:
Start CNTRL TUBE
FILES... Tube:
OPTIONS...
Lid
Incubate
IN SITU
– –/22.0° 10:22:06

14

in situ

in situ

Adapter

4 Maintenance

The

in situ

Adapter does not require regular maintenance.

Contamination can be removed using water or a mild laboratory cleaning
agent. The covering hood should be wiped using a moist cloth only in
order to prevent water or other liquids from entering the lateral ventilation
slits of the double chamber.

Do not allow any parts of the
organic solvents or aggressive chemical. Do not clean the transparent
plastic parts using alcohol or acetone.

in situ

Adapter to come into contact with

5 Technical data

Type of instrument:

Sample capacity: Max. 4 glass slides of 76 x 26 mm

Temperature range: 4 to 99 °C
Temperature distribution 40 °C: ± 0,6 °C

across the adapter: 72 °C: ± 0,6 °C

Temperature-control speed: Heating rate ≥ 1,0 °C/s,

Dimensions of the adapter: Metal carrier: Width: 112 mm

Weight: Metal carrier: approx. 60 g
Conditions of use: 0 to 105 °C,

Storage conditions: –20 to +70 °C,

in situ

Adapter for Mastercycler

or Mastercycler gradient

(3 x 1 inch)

95 °C: ± 1,0 °C

measured on the adapter
Cooling rate ≥ 0,7 °C/s,
measured on the adapter

Depth: 79 mm
Height: 9 mm

Covering hood: Width: 148 mm

25 to 100 % relative humidity

25 to 85 % relative humidity

Depth: 105 mm
Height: 31 mm



15

6 Ordering information

Order number Article

5331 220.002
5331 221.904 Operating manual

0030 127.501
0030 127.510
0030 127.528
0030 127.536

in situ

Adapter

German, English, French

For sample preparation

in situ

Frame, 25 µl

in situ

Frame, 65 µl

in situ

Frame, 125 µl

in situ

Frame, 300 µl

16

Eppendorf Offices

ASEAN

Eppendorf AG
Regional Office in Malaysia
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com.my

AUSTRALIA / NEW ZEALAND

Eppendorf South Pacific Pty. Ltd.
Tel. [phone]
Fax [phone]
E-mail: [e-mail]
Internet: www.eppendorf.com.au

AUSTRIA

Eppendorf AG
c/o Schott Austria
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

BRAZIL

Eppendorf do Brasil Ltda.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com.br

CANADA

Brinkmann Instruments, Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.brinkmann.com

CHINA

Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.cn

FRANCE

EPPENDORF FRANCE S.A.R.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.fr

GERMANY

Eppendorf Vertrieb
Deutschland GmbH
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.de

INDIA

Eppendorf India Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.in

ITALY

Eppendorf s.r.l.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.it

Eppendorf Offices

JAPAN

Eppendorf Japan Co. Ltd.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.jp

NORDIC

Eppendorf Nordic Aps
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.dk

SPAIN

Eppendorf Ibérica S.L.
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.es

SWITZERLAND

Vaudaux-Eppendorf AG
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.com

UNITED KINGDOM

Eppendorf UK Limited
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorf.co.uk

USA

Eppendorf North America
Tel. [phone]
Fax [phone]
E-Mail: [e-mail]
Internet: www.eppendorfna.com

OTHER COUNTRIES

see:
www.eppendorf.com/worldwide

is a registered trademark



In touch with life

eppendorf

Your local distributor: www.eppendorf.com/worldwide

Eppendorf AG · 22331 Hamburg · Germany · Tel. [phone] · Fax [phone]

E-Mail: [e-mail]

Eppendorf North America, Inc. · One Cantiague Road, P.O. Box 1019

Westbury, N.Y. 11590-0207 USA · Tel. [phone] · Toll free phone [phone]

Fax [phone] · E-Mail: [e-mail]

5331 221.904-02/0705 Printed in Germany

Europe, International: Tel. [phone] · E-Mail: [e-mail]

Application Support

North America: Tel. [phone] ext. 2258 · E-Mail: [e-mail]

Asia, Pacific: Tel. [phone] · E-Mail: [e-mail]