Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Mode d'emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Contents
1 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2 Safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3 Function and operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.1 Delivery package and installation . . . . . . . . . . . . . . . . . . . . . . . . 12
3.2 Technical description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.3 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3.4 The
4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
6 Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
in situ
reaction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
9
1 General information
The
in situ
Mastercycler® gradient enables
a specially developed carrier system. This allows the Mastercycler® and
the Mastercycler® gradient to be used for the conv entional PCR technique
in different tube systems as well as for the
The
can accommodate up to four glass slides. To ensure optimum temperature
control of the samples, the entire carrier system also has a detachable
covering hood. This covering hood is transparent and double-walled,
which allows access to the sample at all times and also prevents heat
loss.
The adapter is extremely easy to handle: The carrier system is positioned
onto the thermoblock of the Mastercycler
samples are placed on up to four object carriers, fixed into place and
sealed hermetically prior to the PCR. Eppendorf
ideal sealing system for the
(volumes) (see "Ordering information"). The transparent covering hood is
then closed and the PCR may be started.
Thanks to the wide selection of different tube types, the software is
specially tailored to suit the
quality heat transition necessary for optimal results.
Adapter for the Eppendorf Mastercycler® and the Eppendorf
in situ
Adapter is a high-temperature-conductive metal carrier, which
The
in situ
Adapter must be set up and used in accordance
with the operating manual provided in order to protect both the
user and the device. The instructions in the operating manual
of the Mastercycler® or Mastercycler® gradient must also be
observed.
When operating the device, please observe the instructions
contained in the corresponding applicational regulations!
in situ
PCR reactions to be carried out on
in situ
technique.
®
or Mastercycler® gradient. The
in situ
in situ
PCR and are availab le in diff erent siz es
in situ
Adapter, which guarantees the high-
Frames form the
10