Eppendorf InjectMan NI 2 User Manual

InjectMan® NI 2

Operating Manual

Inhalt / Contents / Sommaire / Indice / Indice

Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Mode d’emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Istruzioni d’impiego . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

Manual de Instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133

EG-Konformitätserklärung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144

EC Conformity Declaration Déclaration de conformité Dichiarazione di conformità CE Declaratión de conformidad CEE

Inhalt / Contents / Sommaire / Indice / Contenido

Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung.

No part of this publication may be reproduced without the prior permission of the copyright owner.

Toute reproduction, complète ou partielle et quel que soit le proèdè est interdiete, sauf autorisation

Ristampa e riproduzione – anche di estratti – solo con autorizzazione.

Reimpresión y copia – incluso parciales – sólo con autorización.

expresse de notre part

2006 Eppendorf AG, Hamburg

eppendorf is a registered trademark.

Printed in Germany

Contents

1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

2 Intended use and safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

2.1 Hazards when unpacking and other information and instructions . . . . . . . . . . . . . . 61

3 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

3.1 Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

3.2 Module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

3.3 Control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

3.3.1 Tool movement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

3.3.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

3.3.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Contents

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

4.2 Assembling the module unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.2.1 Components of the module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.2.2 Angle guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.2.3 Mounting the axial module (A module). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

4.2.4 Angle adjuster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

4.2.5 Assembling the hinged bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

4.3 Mounting the module on the microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

4.3.1 Right and left side mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

4.3.2 Assembling the capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

4.4 Connecting cables – Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

4.4.1 Transmission cable 5181/5246 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

5 User control and mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.1 First injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.2 More information on the mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

5.2.1 Setting up the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

5.2.2 Deﬁnition of Z limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

5.2.3 Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

5.2.4 Capillary exchange. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

5.2.5 Field of movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

5.3 Use of the keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

5.3.1 Coarse/Fine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

5.3.2 Setting the speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

5.3.3 Home . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

5.3.4 Axial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

5.3.5 Pos 1 / Pos 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

5.3.6 Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

5.3.7 Arrow keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

5.3.8 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

5.3.9 Standby . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

5.3.10 Joystick button, triggering of injection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

5.3.11 Injection speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

5.3.12 Turn wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

Contents

5.4 Parameter settings via menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.1 Meanings of parameters in menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.1.1 Main menu options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.2 Parameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.2.1 Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.2.2 Inject . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

5.4.2.3 StepInj . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

5.4.2.4 Home . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

5.4.2.5 Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

5.4.2.6 Install . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

5.4.2.7 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

5.5 Parameter setting via keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

5.5.1 General-Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

5.5.2 Software version polling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

5.5.3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

6 Cleaning and service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.2 Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.3 Decontamination prior to shipping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.4 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

8 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

9 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Contetns

9a Ordering information for North America . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

A Appendix – MultiUser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110

1 Summary

Summary

The Eppendorf InjectMan for use in microinjection.

Its optimum functionality and user-friendliness make the InjectMan® NI 2 the ideal solution for easy injection. This is illustrated particularly by the large number of injected cells.

The Eppendorf InjectMan® NI 2 micromanipulator combines the classic beneﬁts of a mechanical system (direct feeling for tool movements) with the advantages of a precise motorised system.

The direct and sturdy adapter between the module unit and the microscope tripod (optionally positioned at the left or right) and the separate control board prevent oscillation and vibration.

Connection to the FemtoJet®, FemtoJet® express or Transjector 5246 also permits semi-automated injections. The injection motion and pressure are activated simultaneously at the press of a button.

The highly resource-conserving way in which the microcapillaries are injected axially at any desired angle into the cell, and also extracted axially, considerably increases the effectiveness and broadens the range of application of microinjection.

No manual wobble of the capillary can damage the cell. The microelectronics and the software help to conserve the cells to the maximum extent.

The clearly laid out keypad with the additional display also help to avoid operator errors and so protect the preparation and the tool.

The unit can be attached to any commonly used inverted microscopes by way of an appropriate adapter. The capillary can be aligned at any angle and in any direction.

Deﬁned working planes and limits largely eliminate the possibility of damage to the capillaries.

An integral CLEAN/HOME function greatly eases the handling and changing of capillaries.

Simple, self-explanatory user control also permits inexperienced users to begin performing injections into adherent cells quickly and successfully. Additional functions are available for the more experienced user.

NI 2 micromanipulator is specially designed as a micromanipulator

2 Intended use and safety precautions

The InjectMan NI 2 enables an electronically controlled axial injection movement. It is designed for controlled, gentle, semi-automatic microinjection in (adherent) cells and small organisms. By coupling with an external pressure source/external pressure sensor (e.g., FemtoJet (express) or Transjector 5246), the injection movement and injection pressure are monitored, and simultaneously activated at the push of a button. Microelectronics and software control the cell-preserving execution of the microinjection process. The cell wall is minimally perforated, thus avoiding cell lysis (via the potential introduction of excess volumes of liquid). In the optimal scenario, inspecting the work area and injection limits reduces damage to the capillaries. The InjectMan NI 2 is only intended for use in research labs. It may only be operated by qualiﬁed lab personnel.

It is essential to observe the following safety precautions!

•

The device must be commissioned and subsequently used in compliance with the instructions set out in this Manual, in order to protect the user and prevent damage to the device!

•

The device may only be used for the purpose for which it is intended!

•

The device is to be used solely for micromanipulation in accordance with the appropriate legislative requirements!

•

Do not operate the device if there is visible damage to the device itself and/or to its packaging.

•

Before starting up the device, please compare your power supply with the voltage requirements on the identiﬁcation plate!

•

Take care when transporting the control board! Do

not

transport the device by carry the joystick!

•

When installing and dismantling, make sure the weight of the motor module (1.4 kg) can be safely carried! Otherwise injury may be caused, or the device may be damaged!

•

A weight of max. 100 g may be applied to the motor module.

•

The motor modules must not be moved against a mechanical obstacle, as this may damage the drive units.

•

Before transporting the micromanipulator, dismantle the module unit and, if possible, transport it in its original box. The delivered transport guards are to be mounted according to the red supplement sheet.

•

Important! The mains plug must not be plugged into the socket outlet until all connections between the control console, power supply unit and motor module have been made.

Caution – risk of trapping/crushing! Do not reach into areas of the motor modules in which your hand may become trapped (the gap between the guide and the module or between the module and any obstacles), and do not insert any other objects into such gaps!

Caution when mounting tools! Mounting must not be performed with pressure. Glass capillaries can be damaged during mounting if they are not handled carefully. Wear eye protection to prevent the risk of injury from glass splinters!

Intended use and safety precautions

2 Intended use and safety precautions

•

Electrical connections to devices not mentioned in this Manual are permitted only with the

•

consent of the manufacturer!

•

Repairs must be carried out only by authorised Service personnel!

The Manual, or any part of it, may be reproduced only with the written consent of

•

Eppendorf AG.

•

Liability for the functioning of the device shall in all cases be transferred to the owners or operators if the device is serviced or repaired by persons not authorised by the Eppendorf approved Service or if any action is performed which is not in conformance with the designated use of the device. Eppendorf accepts no liability for damage arising from failure to comply with the instructions set out in the Manual. Guarantee and conditions of liability of

Intended use and safety precautions

the terms of sale and delivery of Eppendorf AG are not expanded by the above notes.

Eppendorf AG reserves the right to make technical modiﬁcations to this device!

•

Transfer

•

If the device is passed on to someone else, please include the instruction manual.

•

Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European

•

Community

The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002/96/EC on waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after 13.08.05 in the business-tobusiness sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. They are marked with the following symbol to indicate this.

Caution when mounting tools! When the tool is mounted, the motors are swung out forwards and the capillaries face the front. Handle with care!

Caution! If the work is not carried out properly, for example if glass capillaries contact a surface, they may smash and ﬂying splinters may endanger eyes. You should therefore always protect your eyes, such as by wearing goggles!

Never point capillaries at persons! Capillaries can suddenly be loosened from their capillary grips under high pressure and become a missile.

As disposal regulations within the EU may vary from country to country, please contact your supplier if necessary.

2 Intended use and safety precautions

2.1 Hazards when unpacking and other information and instructions

Before checking that the device has been delivered complete and installing it, please read the following section thoroughly.

•

The control board must not be carried or dragged by the joystick! The joystick is a highly sensitive control element which transmits movement commands in the 0.1 µm range! Always carry the unit by the housing only!

•

Never place the control board upside down on the joystick

•

Treat the motor modules with care!

•

Never transport the motor modules while they are still assembled!

•

Whenever possible, transport the motor modules in their original packaging!

•

Do not tilt the assembled motor modules!

•

After the warm-up phase (at least half an hour) the device is virtually drift-free.

•

After switching on, wait for the initialisation process to complete before operating the joystick. Completion of initialisation is indicated by an acoustic signal or by the LED returning to its standby condition.

•

Enter an injection time >0.0 for the Eppendorf injection system being used, otherwise an error message will be delivered

Intended use and safety precautions

The Eppendorf InjectMan – Control board

– Module unit – Mains power supply unit

Description

3 Description

3.1 Components

NI 2 micromanipulator is made up of three components:

Coarse Fine

Pos 1

Limit

Home

Axial

Pos 2

Standby

Fig. 1: InjectMan® NI 2 micromanipulator, without mains power supply unit Module unit for right side mounting (without tool mount)

3.2 Module unit

The module unit can be mounted at the left or right on any commonly used microscopes by way of an adapter.

The module unit comprises the motor unit for horizontal movement (X, Y axis, X/Y module) and the axial motor unit (A axis). The injection angle is adjusted manually using the angle guide. By means of the angle setting and appropriate mounting, injection angles between 60° and 30° are possible.

Vertical movements (Z movements) are executed by the interaction between the A and X axes.

3 Description

On the A module is the tool mount for the probe holders. Tools with a diameter, or width, of 4 mm and weighing max. 100 g can be inserted, e.g. capillary holders for microinjection.

Description

Fig. 2: Module unit (here right side mounted)

1 Motor unit for horizontal movements (X/Y module) 2 Axial motor unit (A module, without tool mount) 3 A module arc guide

3.3 Control board

The control board accommodates the keypad, the display and the joystick with integral button, as well as the turn wheel on the side. The movements performed on the joystick are transmitted to the module unit. The movement modes, directions and speeds, as well as movement sequences, are pre-set and triggered by way of the keypad, the turn wheel and the joystick.

Underside

The bay underneath the control board accommodates the connector sockets for the module unit and the mains power supply as well as the serial port and the connection for the FemtoJet

and an optional foot

switch.

Note

: Do not place the control board down resting on the joystick! When connecting cables (section 4.4), place the control board on its side on an even surface and secure it against toppling over!

3 Description

3.3.1 Tool movement

Moving the dynamic joysticks moves the capillaries in one plane. The speed of the motion is determined by the speed of the deﬂection movement on the joystick. Releasing the joystick stops

the movement, the joystick springs back to its neutral position.

Description

3.3.2 Keypad

The keypad can be used to make settings or initiate movements directly. Frequently used settings can be entered by means of key combinations. The LEDs indicate the active condition of a function. The display shows the current settings and assists user control with visual aids.

Keys

To understand the key functions, users must have read the detailed Manual.

Some of the key functions are triggered when the keys are released.

Coarse/Fine Speed

selected mode is indicated by the LED associated with the key and by the selected speed on the on-screen display.

Home

Move out of working radius and return to the precise same spot.

Rotating the joystick causes the tool to move up and down.

Clockwise rotation: down Counterclockwise rotation: up

Overlaid movements in all three coordinate directions are possible.

selector key: Toggle between

FINE

and

COARSE

manipulation. The

3 Description

Axial

Activate or disable axial movement.

Pos 1 / Pos 2

Approach a stored position. Store current position by pressing and holding down the key. Clear approached position by pressing and holding down the key.

Limit

Set/cancel limits for injection movement.

Arrow keys

Adjusting the limit.

Description

Toggle between menu and back. Parameters can be selected or changed in the menu using the joystick.

Standby

Switches the device off or on. The function is used as a reset function.

Joystick button

Initiate the Inject function with a set limit.

3.3.3 Display

Description

3 Description

fine X: –1200µm

Y: 300µm Z: –21323µm

Turn wheel

Set the speed in the current speed mode. Display of selected speed on-screen.

In normal operation the display shows the coordinates of the three axes (X, Y, Z; Cartesian) and the pre-set mode with the selected speed at maximum joystick deﬂection. Additional functions are displayed in theactive condition.

Parameter settings can be made and changed in the onscreen menu using the joystick.

coarse X: –1200µm

pos 1 pos2

StepInj user 3 –21323 z-limit+search

Deﬁning the operating parameters and functions, and how to program and change them, is described in section 5.

Y: 300µm Z: –21323µm

axial

▲

Display with all display options.

4 Installation

4.1 Unpacking

– The packaging and the transport guards should be kept for use if the device is transported

again later!

– The control board must not be carried or dragged by the joystick!

– The joystick is a highly sensitive control element which transmits movement commands in

the 0.1 µm range. Always carry the unit by the housing only!

– Never place the control board upside down on the joystick!

– Treat the motor modules with care!

– Only remove the motor modules from their packaging together! Never tug on the module

connecting cable!

– Never transport the motor modules while they are still assembled!

– Whenever possible, transport the motor modules with the transport guards in their original

packaging!

– Do not tilt the assembled motor modules!

The packaging comprises two levels. The ﬁrst level contains the accessories. The bottom level contains the module unit and the control board.

Check that the standard accessories are present and complete.

The standard accessories are: 1 Control board

1 Motor module 1 Mains power supply unit 1 Mains power cable 1 Hinged bracket 1 Set of Allen keys (metric) 2 Screws M5x16 1 FemtoJet 2 Positioning aids for universal capillary holders 1 Operating manual 1 Set of spare screws (2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12)

interface cable (5247)

Installation

Carefully lift the control board and the motor modules out of the packaging using both hands.

1. Loosen the screws and remove the transport guard (yellow).

2. Remove the colored transport guard only after comple- tely mounting the device on the microscope!

4 Installation

4.2 Assembling the module unit

4.2.1 Components of the module unit

Installation

Fig. 3: Module unit, assembled (here right side mounted)

1 Motor unit for horizontal movements (X/Y module) 2 Axial motor unit (A module, without tool mount) 3 A module arc guide

4.2.2 Angle guide

The A module is attached by bolting on the angle guide. Depending on the mounting point of the angle guide, injection angles between 60° and 30° can be set.

For the sake of clarity, the A module is not shown.

Example: The angle guide is attached to the X/Y module in the centre hole, corresponding to 45°. The A module can be adjusted to between 55° and 35° (45°±10°) by turning the spindle.

4 Installation

The hole spacing is 5°. By moving the mounting point

4.2.3 Mounting the axial module (A module)

of the angle guide 5° upwards, the adjustable angle changes each time to 50° to 30°.

By moving the mounting point downwards, angles of up to 60° can be set. This requires sufﬁcient space to be available on the microscope.

The angle guide is already bolted to the A module. The spindle may need to be turned until the desired mounting point (arrow, e.g. here centre hole) is accessible.

Note

face of the A module must be slackened prior to adjusting the angle and must then be re-tightened in order to ensure reliable operation.

Installation

: The orange ﬁxing screw on the narrow front

Set the angle guide with the free hole (2) at the mount (1) of the X/Y unit. The ﬁtting pins must be ﬁtted into the holes

provided. Fix the angle guide to the X/Y module by the M4x10 cheese head screw.

4.2.4 Angle adjuster

Installation

4 Installation

45°

40°

35°

Set the desired angle.

Mounting the angle guide at the centre hole and adjusting the A module to 0° corresponds to an angle of 45°.

To perform the adjustment, the lines on the angle guide are moved to match the outer edge of the A module.

Example – 35°: The angle guide is attached by the centre hole, the bottom edge of the A module is set to the lower 10° marking.

Note

: The mechanically set angle must be entered subsequently in the Install menu! Otherwise Z axis movements cannot be executed correctly! See section 5.4.2.6.

Note

: The orange ﬁxing screw on the narrow face of the A module must be slackened prior to adjusting the angle and must then be re-tightened in order to ensure reliable operation.

4.2.5 Assembling the hinged bracket

Set up the hinged bracket according to the side on

which the module unit is to be mounted. Example of left side mounting: The hinge (2) is positioned at the left outer, aligned upwards and to the rear.

4 Installation

Opening the hinged bracket

0° – 45°

For right side mounting, the hinge must be at the right outer (not visible here, on the rear).

The module unit is mounted from above onto the hinged bracket (1).

Installation

To open the hinged bracket beyond its normal opening angle between 0° and 45° for mounting purposes, push the pin on the hinge side up. The slider is purposely hard to move. The unit is usually only mounted once. The pin can be moved with the 4 mm Allen key.

45° – 90°

The hinged bracket can then be opened to 90°. The mounting holes on the module are then accessible.

90°

Installation

4 Installation

The hinged bracket is opened up and joined to the motor module by its inner inclined surface by means of the M5x16 screw. Close the hinged bracket.

Note

: The A module is not shown.

The module unit for left side mounting is mounted on the hinged bracket.

Right side mounting

The hinge is not visible, the mounting point of the A module is facing the middle of the microscope.

4 Installation

4.3 Mounting the module on the microscope

4.3.1 Right and left side mounting

The module unit mounting is dependent on the chosen side of the microscope.

The module unit can be mounted on the most commonly used microscopes (usually inverted microscopes). The unit is mounted on the microscope tripod by way of a separately ordered microscopespeciﬁc adapter. Mounting of the adapter is described in the relevant mounting instructions. If a different type of mounting is required, special adapters will be needed.

The hinged bracket is mounted on the adapter by one of the two shorter rollers as shown in the illustration (here right side mounting; for the sake of clarity the mounted A module is not shown).

The M5x16 screw is supplied as part of the InjectMan® NI 2 accessory set; the roller is a component of the microscope-speciﬁc adapter. The rollers are adapter-speciﬁc.

The module unit (here on the right) is joined to the adapter by way of the roller with two M5x16 screws. The InjectMan® NI 2 is mounted at the front position.

The screws are component parts of the microscope-speciﬁc adapter.

Note

: In the "Install" menu (section 5.4, subsection 5.4.2.6) the parameters Side = RIGHT Direction = FLAT Angle = e.g. 45° are set in the given sequence according to the mode of mounting.

Installation

4.3.2 Assembling the capillary holder

4 Installation

According to the mounting mode on the left side of the adapter.

Note

: In the "Install" menu (section 5.4, subsection 5.4.2.6) the parameters Side = LEFT Direction = FLAT Angle = e.g. 45° are set in the given sequence according to the mode of mounting.

On the A module is the A head (1) for tool mounting.

It is attached to the A module by two M3x16

screws as shown in the illustration.

The A head can accommodate tools with a

diameter of 4 mm, such as the universal capillary holder (accessory FemtoJet

CellTram).

The universal capillary holder is inserted into the A head after the knurled screw (arrow) has been slackened, and then ﬁxed in place by retightening the knurled screw.

4 Installation

To avoid having to reposition every time a capillary is changed, the positioning aid can be mounted on the universal capillary holder so it ﬁts in the centre hole in the A head.

4.4 Connecting cables – Startup

Note

: The connections between the control board, the mains power supply unit and the motor

module must only be plugged and unplugged with the device switched off.

Note

: Do not place the control board down resting on the joystick! When connecting cables,

place the control board on its side on an even surface and secure it against toppling over!

– The connection to the motor module/injector (e.g. FemtoJet

corresponding connectors, as shown in Figure 4.

– Check the voltage and frequency against the rating plate before connecting the mains

power! – Use only the mains power supply unit supplied by Eppendorf! – The cable is plugged into the mains power supply unit before being connected to the mains.

Important

– Check that the connectors are ﬁrmly attached.

: The main power connection is the last one to be connected.

) is made via the

Installation

Fig. 4: Underside of control board

1 Connection for optional foot switch (SER2; SubD9, male) 2 Connection for injector (FemtoJet 3 Connection for mains power supply unit (

Important

4 Connection for motor module (SubD25, female) 5 Serial port (SER0; SubD9, male) for computer (RS 232)

: Connect only after the other components)

, Transjector 5246) (SER1; SubD9, male)

4.4.1 Transmission cable 5181/5246

For optimum use of the INJECT function (see section 5.1), the InjectMan® NI 2 micromanipulator and the microinjector (e.g. Eppendorf FemtoJet® or Transjector 5246) must be interconnected.

Making the cable connection

– Switch off the device.

– Plug the FemtoJet® interface cable (accessory) or interface cable 5246/5242 (optional) into

– Switch on the device.

Installation

Note

micromanipulator with the Eppendorf microinjector 5242.

4 Installation

socket 2.

The display shows "Injector ready" if the connected injector is ready for operation.

: Interface cable 5246 can be used to combine the Eppendorf InjectMan® NI 2

5 User control and mode of operation

Section 5.1 provides a brief summary of how the commands can be used for the injection procedure. Section 5.2 provides additional information on the device's mode of operation. Sections 5.3 and 5.4 set out detailed descriptions of all the commands.

5.1 First injection

Precondition

Prior to performing the ﬁrst injection, it is assumed that there is a ﬁlled capillary in the correctly positioned capillary holder (see sections 5.2.1 and 4.3.2). Other requirements: The device is properly initialised (pre-set angle, mounting side deﬁned, etc.) and an Eppendorf injector (e.g. FemtoJet The InjectMan® NI 2 micromanipulator is switched on and in the following "start mode": The capillary is at the top position and to the side of the sample. The LED of the "Speed" key is lit in FINE mode. The display shows only the speed setting for FINE, the coordinates for the X, Y and Z axes and "Injector ready". On the microscope table is a focussed sample, e.g. adherent cells in a petri dish, coated with medium.

Movement towards sample

Toggle "Speed" key to COARSE mode.

Coarse

Fine

The capillary is moved to the surface of the medium by pushing/rotating the joystick in the direction of the sample. Visual monitoring of the movement.

The speed can be increased by turning the wheel on the right hand side of the control board. The change of speed is indicated on the display.

) is connected.

fine X: 0µm

Injector ready

coarse X: –1200µm

Injector ready

Y: 0µm Z: 0µm

Y: 300µm Z: –21323µm

COARSE 7500

µm/s

User control and mode of operation

5 User control and mode of operation

The new speed is shown on the display as a modiﬁed block diagram.

Coarse

Fine

right brings the tip of the capillary into the focal plane (microscopic control). Adjust the speed as necessary by way of the turn wheel.

The focal plane is adjusted carefully to the cells. The capillary tip is brought into the focal plane.

The set limit is indicated on the bottom line of the display directly above the "Limit" key. The number before the

User control and mode of operation

limit indicator is the level of the limit. The value is stored. The capillary cannot be moved any lower.

Move the capillary up slightly by rotating the joystick to the left. The height to which the capillary moves is termed the "search plane". By moving the joystick the capillary tip is moved over a cell in the search plane.

injection time has elapsed the capillary is returned to the search plane.

This procedure automatically injects the cell with the pre-set parameters.

If the capillary touches the medium surface for example (visually monitored), switch to FINE mode.

The capillary is moved into the beam path of the microscope by changing the plane of the joystick. Carefully lowering the capillary by rotating the joystick to the

Press the "Limit" key. The Inject function cannot be executed without a deﬁned limit.

Press the joystick button initiates the automatic injection movement. The capillary is moved to the side and then axially to the limit plane.

In parallel with the injection movement, the connected microinjector is activated and the injection pressure is built up. When the microinjector's

coarse X: –3200µm

Injector ready

fine X: –3200µm

Injector ready –21455 z-limit

INJECT

INJECT-Position

Y: 300µm Z: –21323µm

Y: 300µm Z: –21455µm

–3200 300

–21455

Moving to

5 User control and mode of operation

Changing the limit

As a result of moving the petri dish, the limit may need to be adjusted. This is done using the arrow keys to the left and right of the "Limit" key.

Each key press changes the value of the limit upwards or downwards. The new value of the limit is checked by a test injection. Alternatively, the stored limit can be cleared by pressing the "Limit" key again and then be redeﬁned.

Home

To change capillaries, the Home function can be used. Move the capillary up slightly by rotating the joystick to the left. Press

and hold down the "Home" key. Swivel out the motor module and change the capillary. Swivel the motor module back into place and press the "Home" key. The capillary is returned to its home position.

If necessary, the capillary can also be moved manually to the sample by moving the joystick in the desired direction.

Standby

When the operation is complete, the InjectMan® NI 2 micromanipulator can be switched off by the “Standby“ key. The display illumination is switched off and Standby is displayed.

HOME

To move back use HOME

or joystick

STANDBY

–3200 300

–21455

User control and mode of operation

5.2 More information on the mode of operation

5.2.1 Setting up the capillary

– Mount the capillary holder (without capillary) at as high a position as possible on the

A module. Use the positioning aid if necessary.

– From the menu select the CentrMot function (see sections 5.4 and 5.4.7). Make sure there

are no obstacles blocking the ﬁeld of movement! – Run the CentrMot function. The motor unit moves towards the microscope lens. – Swivel out module (see section 5.2.4) – Attach the capillary to the universal capillary holder without dismantling the holder. – Swivel motor back into place.

5 User control and mode of operation

– Carefully slacken knurled screw of tool mount and positioning aid a little way. Carefully bring

the capillary holder with the capillary into the focal plane by hand. If necessary, bring the

capillary into the ﬁeld of view by means of horizontal movements of the joystick (X and Y

axis).

– When the capillary is in the ﬁeld of view, bring it carefully into focus (e.g. at a 4 times zoom

factor). – Tighten knurled screw and positioning aid.

This procedure adjusts the capillary so that approximately 80 % of the ﬁeld of movement is then above the current position. This setting can be used for all capillaries of the same length. By means of the positioning aid (section 4.2.3) the universal capillary holder can be dismantled and reﬁtted in the tool mount as necessary. This does not affect the alignment.

Other setups can also be used speciﬁc to application, without any problems.

5.2.2 Deﬁnition of Z limit

To protect the tool and the preparation against damage, and for the automatic Inject function, a limit must be deﬁned. This is relevant mainly to the application of microinjection into adherent cells.

Once the limit is deﬁned, the capillary is moved upwards manually above the cells. This plane, from which the various cells are injected, is termed the search plane.

User control and mode of operation

Microcapillary Cell

Search plane

Z limit

Glass slide

Fig. 5: Capillary above cell on search plane, Z limit set.

To deﬁne the limit, the capillary is set to the desired position by lightly pressing in the cell.

– Press the "Limit" key.

Microcapillary

Cell

Focal plane

Glass slide

5 User control and mode of operation

Or the capillary is brought into the focal plane like the cell.

– Press the "Limit" key.

Cell

Focal plane

Microcapillary

Glass slide

The deﬁned limit is indicated on the display. To clear the limit, press the "Limit" key again.

To set a limit at a lower position, the limit must ﬁrst be cleared. Only then can the capillary be moved lower and a new limit be deﬁned.

To adapt to uneven surfaces (primarily when working with plastic petri dishes) or to differing cell morphology, the limit can be adjusted upwards or downwards using the arrow keys. Each key press changes the limit by approximately 0.2 µm.

Press and hold down an arrow key to change the limit continuously. Each 1 µm change is signalled acoustically. To check the success of the change, an injection can be triggered directly.

In the special z-limit+search application, the limit changes made also affect the search plane. The capillary changes its Z position by the same distance as the limit is changed.

fine X: –3200µm

Injector ready –21444 z-limit

fine X: –3200µm

Injector ready –21442 z-limit

coarse X: –1200µm

Injector ready –21323 z-limit+search

Y: 300µm Z: –21455µm

Y: 300µm Z: –21346µm

User control and mode of operation

5 User control and mode of operation

5.2.3 Injection

Pressing the joystick button activates the Inject function.

The capillary moves automatically from its current position, the search plane, to the side and

then axially to the limit plane. When the injection is complete the capillary returns by the same route to its starting position. The injection process is shown on the display.

Injection with pre-set Synchronisation Immediate (factory default) is shown schematically in Fig. 6.

1 2

microcapillary

cell

Z-Limit

slide

3 4

injection

User control and mode of operation

search level

Fig. 6: Inject function

1 Starting position, capillary in search plane 2 The manipulator performs a backswing movement 3 The capillary injects in axial direction into the cell as far as the Z limit 4 When the injection is complete the capillary returns to the search plane

Injection options with a connected microinjector (e.g. FemtoJet®; Automatic injection mode, ready; Readout on display of InjectMan® NI 2: Injector ready):

– With Synchronisation Immediate the connected injector in Fig. 6.1 begins to build up

pressure. The states as shown in Fig. 6.3 is maintained until the injector's injection time has

elapsed. – Synchronisation Limit: The pressure build-up only begins when the limit is reached

(Fig. 6.3). When the injection time elapses the capillary is moved back (Fig. 6.4). – Synchronisation Pressure: There is no injection movement, the pressure build-up of the

connected microinjector is started immediately. The injection is made at the location of the

capillary. A manual injection movement can be performed beforehand.

5 User control and mode of operation

– Synchronisation Move: No pressure build-up occurs. If the joystick button is held down the

capillary stops as shown in Fig. 6.3 and a separate injection can be triggered with a

(manual) injector.

If the injection is triggered via the connected injector, the InjectMan

warning. With this synchronisation the movement can be triggered only by way of the

InjectMan® NI 2.

NI 2 delivers a

5.2.4 Capillary exchange

Before swivelling it out, make sure the capillary cannot be impeded by any obstacles! Wear goggles to protect against possible glass splinters! Swivel out module unit on X/Y module.

Swivel module unit for capillary exchange out of working radius. The capillary swivels out forwards and upwards with the module unit.

– Before changing the capillary, bring the capillary to a position over the petri dish either

manually or by way of the Home function. – Grip module unit at rear motor unit (X/Y module) and swivel out. – Do not use the A module! – Exchange capillaries and swivel module unit back into place. – Bring the new capillary back into the focal plane.

Fig. 7: Module unit in working position and swivelled out (here without capillary holder)

User control and mode of operation

5 User control and mode of operation

5.2.5 Field of movement

To utilise the beneﬁts of axial injection to the full, only one motor (A module) is used. This permits less harmful injection, with minimal damage to the cell.

As a result, vertical movements are set with the A module and the X axis together. To perform a downward movement, the X axis is moved outwards and the A module is moved forwards, simultaneously. This results in the downward Z movement.

This has effects on user control of the micromanipulator in the boundary zones of the X axis/Z axis.

In the case of a three-dimensional manipulator with a motor standing for each coordinate, the ﬁeld of movement forms a cube with equal sides.

As a result of the arrangement of the motor axes of the InjectMan® NI 2, this cube is tilted in X direction by the mounting angle of the A module. Consequently, it is not possible to execute purely Z movements at the sharp angled corners of the cube. Here axial movements are necessary, executed by simultaneously moving the joystick back and forth and rotating it.

The actual ﬁeld of movement (provided the capillary is mounted properly) relevant to the experiment is much smaller than the tilted cube. Within this working area, conventional working in the three coordinates is possible.

User control and mode of operation

Fig. 8: a) Cartesian ﬁeld of movement b) Field of movement with axial component

1 Capillary 2 Field of movement in experiment 3 Height of capillary 4 Position of capillary after start of CentrMot function (section 5.4.2.7)

5 User control and mode of operation

5.3 Use of the keypad

Some of the key functions are triggered when the keys are released.

5.3.1 Coarse/Fine

Selector key Toggle between manipulation.

The selected mode is indicated by the LED associated with the key and by the selected speed on the on-screen display ( ).

Speed

FINE

selector key:

and

COARSE

fine X: –1200µm

coarse X: –1200µm

Y: 300µm Z: –21323µm

User control and mode of operation

5 User control and mode of operation

5.3.2 Setting the speed

The speed can be set in three ways: Directly using the turn wheel, by pressing and holding down the key, or by way of the menu.

Turn wheel

Press and hold down the Speed key

Pressing and holding down the "Speed" key in the selected speed mode (Coarse/Fine) activates the setting, which is then also indicated on the display. The speed is

User control and mode of operation

reduced in stages.

Each stage is also indicated acoustically. If you switch directly from the minimum to the maximum radius a long acoustic signal sounds. There are ﬁve different levels, each roughly halving the speed.

FINE 1000 µm/s

The turn wheel can be used to make speed settings directly.

Turn wheel backwards: increase speed to maximum possible Turn wheel forwards: reduce speed to minimum possible The setting is made in stages. Finer settings can be made by

way of the menu.

The turn wheel can also be used to navigate round the menu.

FINE 250 µm/s

5 User control and mode of operation

5.3.3 Home

The tools moves axially out of the working radius. The function is intended for changing capillaries, or for quick removal of capillaries out of the working radius.

HOME: Press (and hold down as necessary) to move out as far as motors' end position. The red LED ﬂashes during the movement, and is then lit steadily when the end position is reached. Press the key again to move the tool back to its starting point. To return the tool manually the joystick can be moved directly. This terminates the Home function.

Clean moves the capillary out of the medium. In the process, cell particles adhering to the medium boundary layer are removed from the capillary.

When Clean is active: CLEAN: Tap to move out a pre-set distance. The red LED ﬂashes during the movement and at the end point. Press the key again to move the tool back precisely to its starting position, or move the tool manually.

If the parameter BackMan in the "Home" menu is set to "off", it is then not possible to terminate the Home or Clean function by moving the joystick (see ﬁgure under notice text). Interruption of the function is possible only by a Reset ("Standby" button).

–3200 300

HOME

To move back use HOME

or joystick

CLEAN

To move back use HOME

or joystick

Home

To move back use HOME

–21455

–3200 300

–21455

–3200 300 –21455

User control and mode of operation

5.3.4 Axial

Activate or disable axial movement Rotating the joystick causes the tool to

move axially up or down. The active function is shown by the lit LED and is indicated on the display.

Clockwise rotation: Movement

diagonally downwards

Counterclockwise rotation: Movement

diagonally upwards

When the Z limit is reached the only movement is in X direction.

coarse X: –1200µm

Y: 300µm Z: –21323µm

axial

5 User control and mode of operation

5.3.5 Pos 1 / Pos 2

Approach

Store

The stored position is shown by the LED and indicated on the display. Reaching of the position is also indicated by the LED and on the display (here position 2, for example).

The current position of the tool is stored in the three spatial coordinates. Storing new positions overwrites existing ones. Positions can also be cleared by performing a reset ("Standby" key) or via the "Position" menu.

5.3.6 Limit

User control and mode of operation

Long key press: see section 5.3.11

holding down the key (acoustic acknowledgement).

Clear

holding down the key again (acoustic acknowledgement).

Set/cancel limits for injection movement.

An indicator on the bottom line of the display above the Limit key indicates whether the limit is set. To the left of it is the stored value for the limit.

a stored position.

current position by pressing and

coarse X: –1200µm

pos 1 pos 2

coarse X: –1200µm

–21323 z-limit

Y: 300µm Z: –21323µm

▲

Y: 300µm Z: –21323µm

▲

5.3.7 Arrow keys

Adjusting the limit.

By moving the petri dish, the position of the sample may shift relative to the limit. The value of the limit can be adjusted. To do so, press the relevant arrow key. The stored limit value changes by approximately 0.2 µm per key press. Press

and hold down the key to change the limit value continuously. In the event of changes by 1 µm (cf. value on display) an additional acoustic signal is delivered.

coarse X: –1200µm

–21324 z-limit

Y: 300µm Z: –21323µm

5 User control and mode of operation

5.3.8 Menu

Toggles between menu and back.

Parameters can be selected or changed in

the menu using the joystick. For a detailed

description see section 5.4.

The left hand column of the display lists the main menu options. The current selected option is indicated by arrows.

The right hand column, under "Submenu", lists the parameters associated with the selected main menu option.

5.3.9 Standby

Switches the device off or on. When the

device is off, the unlit display shows

"Standby".

To switch on, press the "Standby" key

again. The device runs through the power-

up routine.

This functionality is also used as a reset function.

5.3.10 Joystick button, triggering of injection

Triggering the Inject function.

Pressing the joystick button triggers the

automatic injection movement. The

capillary is moved to the side and then

axially to the limit level.

In parallel with the injection movement, the connected microinjector is activated and the injection pressure is built up. When the microinjector's injection time has elapsed the capillary is returned to the search plane.

This procedure automatically injects the cell with the pre-set parameters.

▲

Speed –Submenu –

▲

Inject Coarse StepInj Fine Home InjSpeed Position StpSpeed Install PosSpeed Function HomSpeed

STANDBY

–3200 300

INJECT

INJECT-Position

–21455

Moving to

User control and mode of operation

5 User control and mode of operation

5.3.11 Injection speed

By pressing and holding down the "Limit"

key the injection speed can be adjusted.

The current selected speed is indicated on

with each acoustic signal.

The injection speed can also be ﬁne-adjusted by way of the "Inject" menu option.

5.3.12 Turn wheel

5.4 Parameter settings via menu

User control and mode of operation

The right hand column lists the submenu parameters associated with the option. When a new main menu option is selected the left hand column changes accordingly.

the display. The injection speed is reduced

When the "Menu" key is pressed the main

menu options are listed on the display at

the left. The selected option is framed by

two arrows (cursor).

The main menu options are selected by

moving the joystick back and forth or

rotating it.

Alternatively, the turn wheel can be used.

INJECT 600

Setting the speed in the current speed mode. Display of selected speed on-screen.

The turn wheel can be used to navigate round the menu and select options.

▲

Speed –Submenu – Inject Coarse StepInj Fine Home InjSpeed Position StpSpeed Install PosSpeed Function HomSpeed

Speed –Submenu – Inject Side StepInj Direction Home Angle Position LCDLight

▲

Install Beeper Function MultUser

µm/s

▲

Invert Y

5 User control and mode of operation

Moving joystick to the right moves the

cursor into the submenu.

The left hand column shows the possible

parameters, the right hand column the

associated values or setting options.

Alternatively, the key can be used.

The parameter being changed is selected

by moving the cursor (joystick) back and

forth or rotating it.

Alternatively, the turn wheel can be used.

Moving the joystick to the right moves the

cursor to the parameter value.

Alternatively, the key can be used.

The desired The value is set by moving the

joystick back and forth or rotating it.

Alternatively, the turn wheel can be used.

--Submenu Install --

▲

Side Right Direction Up Angle 45° LCDLight ON Beeper ON MultUser Invert Y OFF

--Submenu Install -Side Right

▲

Dirction Up Angle 45° LCDLight ON Beeper ON MultUser 1 Invert Y OFF

--Submenu Install -Side Right Direction Up Angle 45° LCDLight ON Beeper ON MultUser 1 Invert Y OFF

--Submenu Install -Side Right Direction Flat Angle 45° LCDLight ON Beeper ON MultUser 1 Invert Y OFF

▲

User control and mode of operation

▲

To save the setting, press the joystick button. The cursor jumps back into the parameters column.

Other parameters can be changed as necessary.

Alternatively, the key can be used.

--Submenu Install -Side Right

▲

Dirction Flat Angle 45° LCDLight ON Beeper ON MultUser 1 Invert Y OFF

▲

5 User control and mode of operation

To switch to other main menu options press the joystick button again or

move the joystick to the left.

Alternatively, the or

can be used.

To switch to working mode press the joystick button again or quit the menu by

pressing the "Menu" key.

Alternatively, the key can be used.

You can quit the menu at any time by pressing the "Menu" key.

User control and mode of operation

Menu navigation using keys.

key

Speed –Submenu – Inject Side StepInj Direction Home Angle Position LCDLight

▲

Install Beeper Function MultUser

coarse X: –1200µm

▲

Invert Y

Y: 300µm Z: –21323µm

Limit

Store

5 User control and mode of operation

5.4.1 Meanings of parameters in menu

5.4.1.1 Main menu options

Speed: Includes all speed parameters.

Inject: Includes all the parameters relevant to

injection.

StepInj: Includes all the parameters relevant to the

special "Step Injection" mode.

Home: Includes all the parameters relevant to the

Home and Clean functions.

Position: Includes all the parameters associated with the Position function.

Install: Includes all the parameters required for basic setup, such as during installation.

Function: Includes speciﬁc functions required for tests or special setups.

5.4.2 Parameter

5.4.2.1 Speed

Coarse: Speed with maximum joystick

deﬂection for coarse manipulation. Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

Fine: Speed with maximum joystick

deﬂection for ﬁne manipulation. Adjustable from 1,000 to 5 µm/s in 5-µm/s increments.

InjSpeed: Speed of (axial) injection movement.

Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

StpSpeed: Speed of step injection.

Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

PosSpeed: Speed of movement to stored position.

Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

HomSpeed: Speed of Home and Clean movement.

Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

Speed –Submenu– Inject Coarse StepInj Fine Home InjSpeed Position StpSpeed Install PosSpeed Function HomSpeed

--Submenu Speed --

▲

Coarse 7500µm/s Fine 500µm/s InjSpeed 300µm/s StpSpeed 2500µm/s PosSpeed 4500µm/s HomSpeed 7500µm/s

▲

User control and mode of operation

5 User control and mode of operation

5.4.2.2 Inject

InjSpeed: Speed of (axial) injection movement.

Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

Synchron: Synchronisation of connected

injector (e.g. FemtoJet®). Setting options:

Immediate: The injection pressure is

built upwhen the Inject function is activated.

Limit: The injection pressure is built up when the (Z) limit is reached. Move: No injection pressure is built up;

only the injection movement is executed.

Pressure: The injection pressure is activated;

no injection movement occurs.

The synchronisation also applies to the special StepInj application.

Search+L: When the parameter is active (ON), changing the limit value additionally changes

the position of the tool – and thus of the search plane – by the same value. The distance between the limit and the search plane remains constant. Setting options: ON / OFF

LimAxis: The parameter sets the axis on which the limit is to be used.

User control and mode of operation

InjAxial: The active parameter (ON) causes an axial injection at the pre-set angle.

Setting options: Z-axis / X-axis. The Z axis setting should be chosen for adherent injection. The X axis setting is used for special injections (not currently possible).

If the parameter is disabled, the injection is performed vertically downwards (in Z direction with setting "LimAxis = Z-axis"; with "LimAxis = X-axis" the parameter is irrelevant). Setting options: ON / OFF

--Submenu Inject --

▲

InjSpeed 7500µm/s Synchron LIMIT Search+L OFF LimAxis Z-AXIS InjAxial ON

▲

5 User control and mode of operation

5.4.2.3 StepInj

This special parameter enables the tool to be move forward automatically from its current position by a speciﬁc distance (D) at a speciﬁc speed, to perform an injection and then return to its starting position.

StepInj: Switches the function on or off.

Setting options: OFF / ON

StpSpeed: Speed of

step injection. Adjustable from 7,500 to 5 µm|s in 5-µm/s increments.

StpDist: Distance the tool is to move forward.

Adjustable from 5 to 2,000 µm in 5-µm increments.

StpAxial: Activates or disables the axial movement of the step injection.

Setting options: ON / OFF

The synchronisation set in Inject/Synchron parameter also has an effect on the StepInj command.

5.4.2.4 Home

HomSpeed: Sets the speed for the Home and Clean

movement. Adjustable from 7,500 to 5 µm/s in 5-µm/s increments.

Offset: Sets the height of the position to which the

tool is to move on completion of the Home movement. Adjustable from 0 to 2,000 µm in 5-µm increments. The offset position is on the axial axis of the start point.

Home: Switches the Home function on or off.

Setting options: ON / OFF

Clean: Switches the Clean function on or off.

Setting options: ON / OFF

Distance: Sets the distance to be used for the Clean function.

Only operative when Clean is activated. Adjustable from 0 to 15,000 µm in 5-µm increments.

BackMan: ON: The home position can be left by

moving the joystick. OFF: The home position can be left by pressing the Home key again. All other operations are not possible, except for Reset/Standby.

Parameters are user-dependent.

--Submenu StepInj --

▲

StepInj OFF StpSpeed 2500µm/s StpDist 20µm StpAxial ON

--Submenu Home --

▲

HomSpeed 7500µm/s Offset 2000µm Home ON Clean OFF Distance 3000µm

▲

User control and mode of operation

5 User control and mode of operation

5.4.2.5 Position

PosSpeed: Sets the speed at which the stored position

is approached. Adjustable from 7,500 to 5 µm/s

ClearAll: When the function is run (Execute) all stored

5.4.2.6 Install

The default setting of the InjectMan® NI 2 installation parameters is right, ﬂat and 45°.

Side: Indication of which side of microscope the

Direction: Indicates how the large motor module

User control and mode of operation

Angle: Angle at which the axial module (A module) is mounted to the plane of the

LCDLight: Switches the display illumination on or off.

Beeper: Switches the speaker on or off.

MultUser: Indicates the number of possible users potentially working with differing

Invert Y: The Invert Y parameter can be used to invert the direction of movement of the

in 5-µm/s increments.

positions are cleared.

module unit is mounted on. Adjustable: Right or Left

(X/Y module) is mounted. Adjustable: Horizontal (Flat) or vertical (Up). The setting automatically changes the value for the Angle parameter. Check both parameters! For vertical (Up) different components are required, as well as a different mode of mounting. See ordering information, "Non-standard accessories".

microscope table. Changing the Direction parameter changes the value of the Angle parameter. Check both parameters!

Setting options: ON / OFF

parameters. Setting options: 1 – 4 By way of the MultUser function, parameters can be stored separately speciﬁc to the application and retrieved as needed. Installation parameters are stored globally.

Y axis. This is useful for microscopes that show a mirrored image, e.g. upright microscopes. OFF: The movement of the tool follows the joystick movement. ON: The movement of the tool is in the opposite direction to the joystick movement.

Parameters are independent of user.

--Submenu Position--

▲

PosSpeed 5000µm/s ClearAll Execute

--Submenu Install --

▲

Side RIGHT Direction FLAT Angle 45° LCDLight ON Beeper ON MultiUser 1 Invert Y OFF

▲

5 User control and mode of operation

5.4.2.7 Function

ZeroCord: This function sets the coordinates of the

X, Y and Z axes to zero.

CentrMot: This function sets the motors to a mid

position. See section 5.2.5.

Before executing the function, remove the probe holder and

capillary! Remove objects from the travel range of the micromanipulator! Avoid collisions! If the function is nonetheless executed with a capillary present, make sure it is not destroyed! Wear goggles as a precaution!

If you do not want to execute the function, press the "Menu" key. The current function can be aborted by pressing the "Standby" key.

Selftest: This function triggers a self-test.

Before executing the function remove the probe holder and capillary!

Remove objects from the travel range of the micromanipulator!

Avoid collisions! All motor axes are moved. In case of error an error message is delivered. If you do

not want to execute the function or want to stop it, press the "Menu" key.

MotorOff: This function switches off the motor currents. The motors can no longer be

operated. The tool may move to a preferred position.

UsrDeﬂt: This function resets the user parameters to defaults (User Default). If you do not

want to execute the function, press the "Menu" key.

--Submenu Function--

▲

ZeroCord Execute CentrMot Execute Selftest Execute MotorOff Execute UsrDeflt Execute

▲

User control and mode of operation

5.5 Parameter setting via keys

In addition to the familiar speed setting (press and hold down "Speed" or "Limit" key) and position storage key functions, there are also other special functions operated via the keypad.

5.5.1 General-Reset

The general reset resets the device to its delivery defaults. All the parameters, including the installation parameters and the number of users, are reset to the defaults set at the factory.

Execution: – Switch device to Standby. – Press "Standby" to switch device on again and then immediately press and release "Home"

key.

The device runs through a lengthy initialisation phase.

5 User control and mode of operation

5.5.2 Software version polling

As an additional software version polling facility (also visible during the power-up routine on the display) the version number can be indicated acoustically. The version number is given in three signal sequences, with the digit 0 represented as 10.

Execution: – Switch device to Standby – Press "Standby" to switch device on again and then immediately press and release "Axial"

key.

The device runs through the initialisation phase and the acoustic output begins.

5.5.3 Installation

As an alternative to the Install menu option, the installation can also be performed by way of the keypad.

Execution: – device is on.

– Press "Speed" (Coarse/Fine) and "Axial" keys simultaneously. – The Installation subfunction is activated and is indicated on the display and by the two

LEDs on the "Speed" key being lit.

– The "Home" key is used to enter right or left side

User control and mode of operation

mounting. In addition, a RIGHT setting is indicated by a short acoustic signal and a LEFT setting by a long one. As shown on the display, pressing the Home key would activate left side mounting. (Home =>LEFT)

– The "Pos 1" key is used to enter horizontal or upright

mounting of the X/Y module. In addition, an UP (upright) setting is indicated by a short acoustic signal and a FLAT (horizontal) setting by a long one.

– Pressing and holding down the "Axial" key enters the mounting angle. In addition to the

display readout, the change of angle per 5° increment is indicated by a short acoustic signal, a 90° setting by a long acoustic signal and a 45° setting by a double one.

– Pressing and holding down the "Menu" key initiates loading of a software update. To be

undertaken only by Service!

– Press the "Coarse/Fine" key to terminate installation and save the parameters directly.

The display switches to the routine display and only the LED of the selected speed range is lit.

The device runs through a lengthy initialisation phase.

Coarse

Fine

Coarse

Fine

Axial

Installation

Home => LEFT POS1 => UP AXIAL(long) => 40° Menu(long) => LOAD

Press COARSE to quit

6 Cleaning and service

6.1 Cleaning

Before cleaning the InjectMan

Ensure that no ﬂuids enter into the inside of the micromanipulator in order to avoid short-circuits in the electrical installation as well as corrosion.

Wipe painted parts and aluminum surfaces using a cloth and mild detergent and then with a dry cloth.

Warning

Clean the InjectMan® NI 2 before disinfecting.

Wipe every part of the InjectMan® NI 2, including accessories and connector cables, with an alcohol-based disinfectant (Isopropanol, Alcohol).

Spray disinfection is not advisable, as disinfectants can enter into the micromanipulator.

The disinfection method used must comply with current disinfection regulations and guidelines.

A decontamination certiﬁcate must accompany the InjectMan® NI 2.

A printed form ("Decontamination certiﬁcate for return of goods" and general instructions for decontamination are available at our www.eppendorf.com homepage.

The serial number of the device must be entered in the decontamination certiﬁcate.

: Do not use any corrosive, solvent or abrasive detergents or polishes.

6.2 Disinfection

6.3 Decontamination prior to shipping

If the device is to be inspected, repaired or calibrated by the service partners of the Eppendorf AG, it must be free of hazardous substances and clean!

NI 2, disconnect the plug.

Cleaning and service

6.4 Service

The dealer is responsible for warranty and service.

7 Troubleshooting

The following error situations are conceivable:

– Motor axes move in wrong direction or do not

Troubleshooting

match joystick movement.

– Parameters for certain user actions are not

accessible.

– Machine not responding to key presses,

Home function still active.

– Tool does not move far enough down. – Cancel limit function

– Tool does not move straight downwards

when joystick is rotated, but also moves to the side.

– Tool moves straight downwards during

injection

– When connected, machine has no display

and will not switch on

Remedy

– Check installation parameters match

motor module setup, e.g. left side mounting etc.

– Enter parameters again or perform a

general reset and then reset the machine.

– Press "Home" key again,

tool moves downwards or move joystick.

or re-adjust tool

– Disable AXIAL function.

– Adapt InjAxial parameter.

– Check mains cable and plug.

If mains power supply unit defective: Service

100

7 Troubleshooting

The following warnings or error messages may be displayed:

Remedy

– Warning 001

Check Injector and cable: The injector is not ready or there is no connection to the theoretically connected injector. Long injection path triggered at injector, timeout of manipulator

– Warning 002

Sync not immediate, limit or pressure: An injection was triggered on the connected injector even though the Move parameter is entered on the InjectMan injection synchronisation.

– Warning 004

Connect motor module: Occurs in self-test. The reason for this warning is most likely that the motor module is not connected to the control board.

– Error 010 – 020

Certain hardware components of the control board are missing.

– Error 021 – 023

Joystick hardware fault.

– Error 024

Wrong internal joystick parameters.

– Error 025

For speciﬁc joystick axes wrong values are deﬁned. Reason may be an incorrect joystick setting on power-up or in a general reset.

– Error 030 – 033

Internal software errors.

– Error 034 – 039

Internal hardware faults.

– Error 040 – 041

Internal software errors.

– Error 042 – 043

Hardware faults

NI 2 as the

– Make the injector ready or check the

connecting cable.

– Trigger "Inject" at manipulator with

long injection paths.

– Do not trigger the injection by the

injector in this case or change the Synchon parameter.

– Connect motor module.

– Service

– Reset, general reset.

If error recurs: Service

– General reset with joystick at mid

position. If error recurs: Service

– Reset, general reset.

If error recurs: Service

– If recurring: Service

– Reset, general reset.

If error recurs: Service

– If recurring: Service

Troubleshooting

101

7 Troubleshooting

– Error 044 – 046

In self-test wrong values deﬁned for

Troubleshooting

displayed axis.

– Error 060 – 062

False data has been imported via the serial ports. There may be errors in the external PC software.

– Error 098

Unable to write to EEPROM: No internal data can be written to the machine's EEPROM.

– Error 099

Internal Error: Internal error

Remedy

– Repeat self-test, reset, general reset.

If error recurs: Service

– Transfer correct data.

– General reset or Service (new

software download or hardware).

– Reset, general reset.

If error recurs: Service

102

8 Technical data

Power supply

Input: 120 – 240 V AC; 50 – 60 Hz; < 50 W Output: 15 – 18 V DC; 25 W

Control board

Contamination level: Overvoltage category: Pollution degree: 2 (IEC 664) Ambient temperature: max. 40 °C Ambient relative humidity: max. 80 %

Weights

Control board with power supply: 2.7 kg Module unit: 1.4 kg, incl. guide

Dimensions

XY module: 120 x 100 x 68 mm A module: 120 x 60 x 50 mm Control board: 270 x 205 x 160 mm

Max. travel: > 18 mm (each axis)

Resolution per microstep: approx. 40 nm

Max. tool travel speed: 7,500 µm/s

The device carries the mark.

15 – 18 V DC; 25 W

(IEC 61010-1)

Technical data

Technical speciﬁcations subject to change!

103

9 Ordering information

InjectMan® NI 2, Europe 5181 000.017 InjectMan® NI 2, USA 5181 000.025

InjectMan InjectMan® NI 2, Japan 5181 000.041 InjectMan InjectMan® NI 2, China 5181 000.084

Delivery package

1 Control board 1 Motor module 1 Mains power supply unit 1 Mains power cable 1 Hinged bracket

Ordering information

1 Set of Allen keys (metric) 2 Screws M5x16 1 Interface cable FemtoJet 2 Positioning aids for universal capillary holders 5171 130.001 1 Operating manual 5181 900.018 1 Set of spare screws 2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12

Adapters

Adapter for Leica DMIL 5181 201.004 Adapter for Leica DMIRE2 5181 202.000 Adapter for Leica DMI 6000 5181 203.007 Adapter for Nikon TMD 5181 210.003 Adapter for Nikon TS-100 5181 212.006 Adapter for Nikon Ti-E/-U/-S 5181 214.009 Adapter for Olympus IX 50/70 IX 51/71/81 5181 220.009 Adapter for Olympus IMT-2 5181 221.005 Adapter for Olympus CK 30/40 / CKX 31/41 5181 222.001 Adapter for Zeiss 100/135/100 S 5181 230.004 Adapter for Zeiss 10/35 5181 233.003 Adapter for Zeiss Axiovert 200 / AxioObserver 5181 235.006 Adapter for Zeiss 25/25C / 40C/40CFL 5181 237.009 Hund Wilovert 30 5181 234.000 Universal stand 5181 250.005 Extension plate 5181 236.002

NI 2, UK/Hongkong 5181 000.033

NI 2, Australia 5181 000.076

Order no.

(5247) 5181 070.015

104

9 Ordering information

Accessories

Universal capillary holder for accommodating Femtotips®, Femtotips® II or grip heads

Grip head 0 for universal capillary holder for microcapillaries with an outer diameter of 1.0 to 1.1 mm

Grip head 1 for universal capillary holder for microcapillaries with an outer diameter of 1.2 to 1.3 mm

Grip head 2 for universal capillary holder for microcapillaries with an outer diameter of 1.4 to 1.5 mm

Grip head 3 for universal capillary holder for microcapillaries with an outer diameter of 0.7 to 0.9 mm

O-Ring Set for grip heads 0 – 3 with removal tool

Service kit for grip heads and capillary holder, consisting of O-Ring Set, adapter for Femtotips

20 Femtotips

20 Femtotips® II 5242 957.000

2x 96 Microloader 5242 956.003

Interface cable 5246/5242 5181 150 060

Foot control 5181 5181 150.051

Arc guide 5181 150.019

"A" head (used with arc guide) 5181 150.027

PC cable update 5181 150.094

Service kit (Screws M3x8, M3x16, M4x10, M4x14, M5x16 and war. M4, M5, M8)

Straight-line guide to connect X module to Y/Z module 5181 150.035

"X" head with angle adjuster 5181 150.043

Spacer (41 mm) for Universal Stand 5181 255.007

(non-standard)

Order no.

5176 190.002

5176 210.003

5176 212.006

5176 214.009

5176 207.002

5176 196.000

5176 195.004

5242 952.008

5181 150.310

Ordering information

Microinjection

FemtoJet® Programmable microinjector with integrated pressure supply

Version Europe 5247 000.013

Version USA and Canada 5247 000.056

Version UK 5247 000.030

Version Japan 100 V, 50 – 60 Hz 5247 000.048

Version Australia 5247 000.072

Version China 5247 000.080

105

9 Ordering information

FemtoJet® express Programmable microinjector incl. connection for external pressure supply

Version Europe 5248 000.017 Version USA and Canada 5248 000.050 Version UK/Hongkong 5248 000.033 Version Japan 5248 000.041 Version Australia 5248 000.076 Version China 5248 000.084

Cell transfer

CellTram Oil manual piston pump for transferring cells (sperms, ES cells) and as manual injector with universal capillary holder for

Ordering information

capillaries with an outer diameter of 1 mm CellTram vario

manual piston pump with variable transmission (1:1, 1:10) to transfer cells (sperms, ES cells) and as manual injector with universal capillary holder for capillaries with an outer diameter of 1 mm

CellTram Air manual piston pump for holding cells (e.g. oocytes) with universal capillary holder for capillaries with an outer diameter of 1 mm

25 VacuTips sterile glass capillaries for holding cells, inner diameter 15 µm, outer diameter 100 µm, 35° angle

25 TransferTips® (ES) sterile glass capillaries for transferring ES cells, inner diameter 15 µm, outer diameter 20 µm, with spike, 20° angle

Headstage holder for preampliﬁers HEKA EPC 7 / EPC9 and AXON CV-4 HS-2

Order no.

5176 000.025

5176 000.033

5176 000.017

5175 108.000

5175 107.004

5173 071.002

106

9a Ordering information for North America

Order no.

InjectMan® NI 2, USA 920000029 InjectMan® NI 2, international 5181 000.017 InjectMan InjectMan® NI 2, Japan 5181 000.041 InjectMan InjectMan® NI 2, China 5181 000.084

Delivery package

1 Control board 1 Motor module 1 Mains power supply unit 1 Mains power cable 1 Hinged bracket 1 Set of Allen keys (metric) 2 Screws M5x16 1 Interface cable FemtoJet 2 Positioning aids for universal capillary holders 920005829 1 Operating manual 5181 900.018 1 Set of spare screws 2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12

Adapters

Adapter for Leica DMIL 920008500 Adapter for Leica DMIRE2 920008526 Adapter for Leica DMI 6000 920008496 Adapter for Nikon TMD 920008534 Adapter for Nikon TS-100 920008551 Adapter for Nikon Ti-E/-U/-S 920008658 Adapter for Olympus IX 50/70 IX 51/71/81 920008585 Adapter for Olympus IMT-2 920008577 Adapter for Olympus CK 30/40 / CKX 31/41 920008569 Adapter for Zeiss 100/135/100 S 920008607 Adapter for Zeiss 10/35 920008593 Adapter for Zeiss Axiovert 200 / AxioObserver 920008615 Adapter for Zeiss 25/25C / 40C/40CFL 920008666 Hund Wilovert 30 5181 234.000 Universal stand 920008631 Extension plate 920008674

NI 2, UK/Hongkong 5181 000.033

NI 2, Australia 5181 000.076

(5247) 920005845

Ordering information for North America

107

9a Ordering information for North America

Accessories

Universal capillary holder for accommodating Femtotips®,

Femtotips® II or grip heads

Grip head 0 for universal capillary holder for microcapillaries with an outer diameter of 1.0 to 1.1 mm

Grip head 1 for universal capillary holder for microcapillaries with an outer diameter of 1.2 to 1.3 mm

Grip head 2 for universal capillary holder for microcapillaries with an outer diameter of 1.4 to 1.5 mm

Grip head 3 for universal capillary holder for microcapillaries with an outer diameter of 0.7 to 0.9 mm

O-Ring Set for grip heads 0 – 3 with removal tool

Service kit for grip heads and capillary holder, consisting of O-Ring Set, adapter for Femtotips

20 Femtotips

20 Femtotips® II 930000043

2x 96 Microloader 930001007

Interface cable 5246/5242 920005853

Foot control 5181 920005799

Ordering information for North America

Curved guide 920005802

"A" head (used with curved guide) 920005748

PC cable update 920005837

Service kit (Screws M3x8, M3x16, M4x10, M4x14, M5x16 and war. M4, M5, M8)

Straight-line guide to connect X module to Y/Z module 920005756

"X" head with angle adjuster 920005764

Spacer (41 mm) for Universal Stand 920008666

(non-standard)

Order no.

920007392

920007414

910012509

910012517

920007406

920005870

920005888

930000035

920005781

Microinjection

FemtoJet® Programmable microinjector with integrated pressure supply

Version USA and Canada 920010504

Version Europe 5247 000.013

Version UK 5247 000.030

Version Japan 100 V, 50 – 60 Hz 5247 000.048

Version Australia 5247 000.072

Version China 5247 000.080

108

9a Ordering information for North America

Order no.

FemtoJet® express Programmable microinjector incl. connection for external pressure supply

Version USA and Canada 5248 000.050

Version Europe 5248 000.017

Version UK/Hongkong 5248 000.033

Version Japan 5248 000.041

Version Australia 5248 000.076

Version China 5248 000.084

Cell transfer

CellTram Oil manual piston pump for transferring cells (sperms, ES cells) and as manual injector with universal capillary holder for capillaries with an outer diameter of 1 mm

CellTram vario manual piston pump with variable transmission (1:1, 1:10) to transfer cells (sperms, ES cells) and as manual injector with universal capillary holder for capillaries with an outer diameter of 1 mm

CellTram Air manual piston pump for holding cells (e.g. oocytes) with universal capillary holder for capillaries with an outer diameter of 1 mm

25 VacuTips sterile glass capillaries for holding cells, inner diameter 15 µm, outer diameter 100 µm, 35° angle

25 TransferTips® (ES) sterile glass capillaries for transferring ES cells, inner diameter 15 µm, outer diameter 20 µm, with spike, 20° angle

Headstage holder for preampliﬁers HEKA EPC 7 / EPC9 and AXON CV-4 HS-2

920002030

920002111

920002021

930001015

930001040

920003168

Ordering information for North America

109

A Appendix – MultiUser

MultiUser

From the Install submenu up to 4 Users (users/applications) can be activated. The Users can be assigned dedicated parameters for speed, injection parameters etc. Non-User-speciﬁc parameters such as installation parameters apply globally, and when changed the changes apply to all Users.

MultUser

Indicates the number of possible users potentially working with differing parameters.

Setting options: 1 – 4 By way of the MultUser function, parameters can

be stored separately speciﬁc to the application and retrieved as needed. Installation parameters are stored globally.

--Submenu Install -Side Right Dirction Up Angle 0° LCDLight ON Beeper ON MultUser Invert Y OFF

▲

When MultUser = 1 is set the part in the display is blank. Where there are multiple Users, the routine display indicates the relevant User, e.g. User 2.

Users can only be changed by switching to Standby and restarting.

Display after power-up if 2 or more Users are activated:

The User value for the desired application or user is set by means of the turn wheel at the side.

Select User

User:2

Turn speed to

select

Press Home to cont.

After setting the desired User press the "Home" key.

The routine display now additionally indicates the User you have set:

Note

: The number of Users can only be reset to 1 by way

of User 1.

coarse X: –1200µm

Y: 300µm Z: –21323µm

user 2

The speciﬁc parameters of the individual users are retained even when the MultUser parameter is set to 1. When an additional user, such as User 2, is reactivated the parameters are then available once again.

The Multiuser parameters can be deleted and reset by way of a general reset, though this will also reset all global parameters, such as installation parameters, to their factory defaults.

110

eppendorf is a registered trademark.

B 5181 900.018-08/042012 · Printed in Germany

Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 53801-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com

Application Support Europe: Tel: +49 1803 666 789 (Preis je nach Tarif im Ausland; 9 ct/min aus dem dt. Festnetz; Mobilfunkhöchstpreis 42 ct/min)

Eppendorf North America, Inc. · 102 Motor Parkway · Hauppauge, N.Y. 11788-5178 · USA

Tel: +1 516 334 7500 · Toll free phone: +1 800-645-3050 · Fax: +1 516 334 7506 · E-mail: info@eppendorf.com

Your local distributor: www.eppendor f.com/worldwide

North America: Tel: +1 800 645 3050 · E-mail: techserv@eppendorf.com

Asia Pacific: Tel: +60 3 8023 6869 · E-mail: support_asiapacific@eppendorf.com

support@eppendorf.com

Eppendorf InjectMan NI 2 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual