Eppendorf InjectMan NI 2 User Manual

InjectMan® NI 2

Operating Manual

Inhalt / Contents / Sommaire / Indice / Indice

Bedienungsanleitung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Operating Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

Mode d’emploi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Istruzioni d’impiego . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122

Manual de Instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133

EG-Konformitätserklärung. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144

EC Conformity Declaration
Déclaration de conformité
Dichiarazione di conformità CE
Declaratión de conformidad CEE

Inhalt / Contents / Sommaire / Indice / Contenido

Nachdruck und Vervielfältigung – auch auszugsweise – nur mit Genehmigung.

No part of this publication may be reproduced without the prior permission of the copyright owner.

Toute reproduction, complète ou partielle et quel que soit le proèdè est interdiete, sauf autorisation

Ristampa e riproduzione – anche di estratti – solo con autorizzazione.

Reimpresión y copia – incluso parciales – sólo con autorización.

Copyright

expresse de notre part

©

2006 Eppendorf AG, Hamburg

eppendorf is a registered trademark.

Printed in Germany

2

Contents

1 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

2 Intended use and safety precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

2.1 Hazards when unpacking and other information and instructions . . . . . . . . . . . . . . 61

3 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

3.1 Components. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

3.2 Module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

3.3 Control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

3.3.1 Tool movement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

3.3.2 Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

3.3.3 Display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Contents

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

4.2 Assembling the module unit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.2.1 Components of the module unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.2.2 Angle guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

4.2.3 Mounting the axial module (A module). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

4.2.4 Angle adjuster . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

4.2.5 Assembling the hinged bracket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

4.3 Mounting the module on the microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

4.3.1 Right and left side mounting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

4.3.2 Assembling the capillary holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

4.4 Connecting cables – Startup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

4.4.1 Transmission cable 5181/5246 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

5 User control and mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.1 First injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

5.2 More information on the mode of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

5.2.1 Setting up the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

5.2.2 Definition of Z limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

5.2.3 Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

5.2.4 Capillary exchange. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

5.2.5 Field of movement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

5.3 Use of the keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

5.3.1 Coarse/Fine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

5.3.2 Setting the speed . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

5.3.3 Home . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

5.3.4 Axial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

5.3.5 Pos 1 / Pos 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

5.3.6 Limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

5.3.7 Arrow keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

5.3.8 Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

5.3.9 Standby . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

5.3.10 Joystick button, triggering of injection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

5.3.11 Injection speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

5.3.12 Turn wheel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

56

Contents

5.4 Parameter settings via menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.1 Meanings of parameters in menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.1.1 Main menu options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.2 Parameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.2.1 Speed. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

5.4.2.2 Inject . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

5.4.2.3 StepInj . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

5.4.2.4 Home . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

5.4.2.5 Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

5.4.2.6 Install . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96

5.4.2.7 Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

5.5 Parameter setting via keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

5.5.1 General-Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97

5.5.2 Software version polling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

5.5.3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 98

6 Cleaning and service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.2 Disinfection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.3 Decontamination prior to shipping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

6.4 Service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

8 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103

9 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104

Contetns

9a Ordering information for North America . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

A Appendix – MultiUser. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110

57

1 Summary

1

Summary

The Eppendorf InjectMan
for use in microinjection.

Its optimum functionality and user-friendliness make the InjectMan® NI 2 the ideal solution for
easy injection. This is illustrated particularly by the large number of injected cells.

The Eppendorf InjectMan® NI 2 micromanipulator combines the classic benefits of a
mechanical system (direct feeling for tool movements) with the advantages of a precise
motorised system.

The direct and sturdy adapter between the module unit and the microscope tripod (optionally
positioned at the left or right) and the separate control board prevent oscillation and vibration.

Connection to the FemtoJet®, FemtoJet® express or Transjector 5246 also permits
semi-automated injections. The injection motion and pressure are activated simultaneously at
the press of a button.

The highly resource-conserving way in which the microcapillaries are injected axially at any
desired angle into the cell, and also extracted axially, considerably increases the effectiveness
and broadens the range of application of microinjection.

No manual wobble of the capillary can damage the cell. The microelectronics and the software
help to conserve the cells to the maximum extent.

The clearly laid out keypad with the additional display also help to avoid operator errors and so
protect the preparation and the tool.

The unit can be attached to any commonly used inverted microscopes by way of an appropriate
adapter. The capillary can be aligned at any angle and in any direction.

Defined working planes and limits largely eliminate the possibility of damage to the capillaries.

An integral CLEAN/HOME function greatly eases the handling and changing of capillaries.

Simple, self-explanatory user control also permits inexperienced users to begin performing
injections into adherent cells quickly and successfully. Additional functions are available for the
more experienced user.

®

NI 2 micromanipulator is specially designed as a micromanipulator

58

2 Intended use and safety precautions

The InjectMan NI 2 enables an electronically controlled axial injection movement. It is designed
for controlled, gentle, semi-automatic microinjection in (adherent) cells and small organisms. By
coupling with an external pressure source/external pressure sensor (e.g., FemtoJet (express) or
Transjector 5246), the injection movement and injection pressure are monitored, and simulta­neously activated at the push of a button. Microelectronics and software control the cell-pre­serving execution of the microinjection process. The cell wall is minimally perforated, thus
avoiding cell lysis (via the potential introduction of excess volumes of liquid). In the optimal sce­nario, inspecting the work area and injection limits reduces damage to the capillaries.
The InjectMan NI 2 is only intended for use in research labs. It may only be operated by qualified
lab personnel.

It is essential to observe the following safety precautions!

•

The device must be commissioned and subsequently used in compliance with the instruc­tions set out in this Manual, in order to protect the user and prevent damage to the device!

•

The device may only be used for the purpose for which it is intended!

•

The device is to be used solely for micromanipulation in accordance with the appropriate
legislative requirements!

•

Do not operate the device if there is visible damage to the device itself and/or to its
packaging.

•

Before starting up the device, please compare your power supply with the voltage
requirements on the identification plate!

•

Take care when transporting the control board!
Do

not

transport the device by carry the joystick!

•

When installing and dismantling, make sure the weight of the motor module (1.4 kg) can be
safely carried! Otherwise injury may be caused, or the device may be damaged!

•

A weight of max. 100 g may be applied to the motor module.

•

The motor modules must not be moved against a mechanical obstacle, as this may damage
the drive units.

•

Before transporting the micromanipulator, dismantle the module unit and, if possible,
transport it in its original box. The delivered transport guards are to be mounted according
to the red supplement sheet.

•

•

•

Important!
The mains plug must not be plugged into the socket outlet until all connections
between the control console, power supply unit and motor module have been
made.

Caution – risk of trapping/crushing!
Do not reach into areas of the motor modules in which your hand may become
trapped (the gap between the guide and the module or between the module
and any obstacles), and do not insert any other objects into such gaps!

Caution when mounting tools!
Mounting must not be performed with pressure. Glass capillaries can be
damaged during mounting if they are not handled carefully. Wear eye
protection to prevent the risk of injury from glass splinters!

2

Intended use and safety precautions

59

2 Intended use and safety precautions

•

•

2

•

Electrical connections to devices not mentioned in this Manual are permitted only with the

•

consent of the manufacturer!

•

Repairs must be carried out only by authorised Service personnel!

The Manual, or any part of it, may be reproduced only with the written consent of

•

Eppendorf AG.

•

Liability for the functioning of the device shall in all cases be transferred to the owners or
operators if the device is serviced or repaired by persons not authorised by the Eppendorf
approved Service or if any action is performed which is not in conformance with the
designated use of the device. Eppendorf accepts no liability for damage arising from failure
to comply with the instructions set out in the Manual. Guarantee and conditions of liability of

Intended use and safety precautions

the terms of sale and delivery of Eppendorf AG are not expanded by the above notes.

Eppendorf AG reserves the right to make technical modifications to this device!

•

Transfer

•

If the device is passed on to someone else, please include the instruction manual.

•

Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European

•

Community

The disposal of electrical devices is regulated within the European Community by national
regulations based on EU Directive 2002/96/EC on waste electrical and electronic equipment
(WEEE).

According to these regulations, any devices supplied after 13.08.05 in the business-to­business sphere, to which this product is assigned, may no longer be disposed of in
municipal or domestic waste. They are marked with the following symbol to indicate this.

Caution when mounting tools!
When the tool is mounted, the motors are swung out forwards and the
capillaries face the front. Handle with care!

Caution!
If the work is not carried out properly, for example if glass capillaries contact
a surface, they may smash and flying splinters may endanger eyes. You should
therefore always protect your eyes, such as by wearing goggles!

Never point capillaries at persons!
Capillaries can suddenly be loosened from their capillary grips under high
pressure and become a missile.

As disposal regulations within the EU may vary from country to country, please
contact your supplier if necessary.

60

2 Intended use and safety precautions

2.1 Hazards when unpacking and other information and instructions

Before checking that the device has been delivered complete and installing it, please read the
following section thoroughly.

•

The control board must not be carried or dragged by the joystick!
The joystick is a highly sensitive control element which transmits movement commands in
the 0.1 µm range! Always carry the unit by the housing only!

•

Never place the control board upside down on the joystick

•

Treat the motor modules with care!

•

Never transport the motor modules while they are still assembled!

•

Whenever possible, transport the motor modules in their original packaging!

•

Do not tilt the assembled motor modules!

•

After the warm-up phase (at least half an hour) the device is virtually drift-free.

•

After switching on, wait for the initialisation process to complete before operating the joy­stick. Completion of initialisation is indicated by an acoustic signal or by the LED returning
to its standby condition.

•

Enter an injection time >0.0 for the Eppendorf injection system being used, otherwise an
error message will be delivered

2

Intended use and safety precautions

61

The Eppendorf InjectMan
– Control board

3

– Module unit
– Mains power supply unit

Description

3 Description

3.1 Components

10

5

5

10

®

NI 2 micromanipulator is made up of three components:

Coarse
Fine

Pos 1

Menu

I

n

j

e

c

t

M

a

n

N

I

2

Limit

Home

Axial

Pos 2

Standby

Fig. 1: InjectMan® NI 2 micromanipulator, without mains power supply unit
Module unit for right side mounting (without tool mount)

3.2 Module unit

The module unit can be mounted at the left or right on any commonly used microscopes by way
of an adapter.

The module unit comprises the motor unit for horizontal movement (X, Y axis,
X/Y module) and the axial motor unit (A axis). The injection angle is adjusted manually using the
angle guide. By means of the angle setting and appropriate mounting, injection angles between
60° and 30° are possible.

Vertical movements (Z movements) are executed by the interaction between the A and X axes.

62

3 Description

On the A module is the tool mount for the probe holders. Tools with a diameter, or width, of
4 mm and weighing max. 100 g can be inserted, e.g. capillary holders for microinjection.

10

5

1

2

3

Description

5

10

3

Fig. 2: Module unit (here right side mounted)

1 Motor unit for horizontal movements (X/Y module)
2 Axial motor unit (A module, without tool mount)
3 A module arc guide

3.3 Control board

The control board accommodates the keypad, the display and the joystick with integral button,
as well as the turn wheel on the side. The movements performed on the joystick are transmitted
to the module unit. The movement modes, directions and speeds, as well as movement
sequences, are pre-set and triggered by way of the keypad, the turn wheel and the joystick.

Underside

The bay underneath the control board
accommodates the connector sockets for
the module unit and the mains power supply
as well as the serial port and the connection
for the FemtoJet

®

and an optional foot

switch.

Note

: Do not place the control board down
resting on the joystick! When connecting
cables (section 4.4), place the control board
on its side on an even surface and secure it
against toppling over!

63

3 Description

3.3.1 Tool movement

Moving the dynamic joysticks moves the capillaries in one plane.
The speed of the motion is determined by the speed of the
deflection movement on the joystick. Releasing the joystick stops

3

the movement, the joystick springs back to its neutral position.

Description

3.3.2 Keypad

The keypad can be used to make settings or initiate movements directly. Frequently used
settings can be entered by means of key combinations. The LEDs indicate the active condition
of a function. The display shows the current settings and assists user control with visual aids.

Keys

To understand the key functions, users must have read the detailed Manual.

Some of the key functions are triggered when the keys are released.

Coarse/Fine
Speed

selected mode is indicated by the LED associated with the key and by the
selected speed on the on-screen display.

Home

Move out of working radius and return to the precise same spot.

Rotating the joystick causes the tool to move up and down.

Clockwise rotation: down
Counterclockwise rotation: up

Overlaid movements in all three coordinate directions are
possible.

selector key: Toggle between

FINE

and

COARSE

manipulation. The

64

3 Description

Axial

Activate or disable axial movement.

Pos 1 / Pos 2

Approach a stored position.
Store current position by pressing and holding down the key.
Clear approached position by pressing and holding down the key.

Limit

Set/cancel limits for injection movement.

Arrow keys

Adjusting the limit.

3

Description

Menu

Toggle between menu and back. Parameters can be selected or changed in
the menu using the joystick.

Standby

Switches the device off or on.
The function is used as a reset function.

Joystick button

Initiate the Inject function with a set limit.

65

3

3.3.3 Display

Description

3 Description

fine X: –1200µm

Y: 300µm
Z: –21323µm

Turn wheel

Set the speed in the current speed mode.
Display of selected speed on-screen.

In normal operation the display shows the coordinates
of the three axes (X, Y, Z; Cartesian) and the pre-set
mode with the selected speed at maximum joystick
deflection. Additional functions are displayed in
theactive condition.

Parameter settings can be made and changed in the on­screen menu using the joystick.

coarse X: –1200µm

pos 1 pos2

StepInj user 3
–21323 z-limit+search

Defining the operating parameters and functions, and how to program and change them, is
described in section 5.

Y: 300µm
Z: –21323µm

axial

▲

▲

Display with all display options.

66

4 Installation

4.1 Unpacking

– The packaging and the transport guards should be kept for use if the device is transported

again later!

– The control board must not be carried or dragged by the joystick!

– The joystick is a highly sensitive control element which transmits movement commands in

the 0.1 µm range. Always carry the unit by the housing only!

– Never place the control board upside down on the joystick!

– Treat the motor modules with care!

– Only remove the motor modules from their packaging together! Never tug on the module

connecting cable!

– Never transport the motor modules while they are still assembled!

– Whenever possible, transport the motor modules with the transport guards in their original

packaging!

– Do not tilt the assembled motor modules!

The packaging comprises two levels. The first level contains the accessories. The bottom level
contains the module unit and the control board.

Check that the standard accessories are present and complete.

The standard accessories are:
1 Control board

1 Motor module
1 Mains power supply unit
1 Mains power cable
1 Hinged bracket
1 Set of Allen keys (metric)
2 Screws M5x16
1 FemtoJet
2 Positioning aids for universal capillary holders
1 Operating manual
1 Set of spare screws
(2 x M5x16, 2 x M4x10, 2 x M3x16, 2 x M3x12)

®

interface cable (5247)

4

Installation

Carefully lift the control board and the motor modules out of the packaging using both hands.

1. Loosen the
screws and
remove the
transport guard
(yellow).

2. Remove the colored
transport guard
only after comple-
tely mounting the
device on the
microscope!

67

10

10

5

5

4 Installation

4.2 Assembling the module unit

4.2.1 Components of the module unit

4

10

5

Installation

5

10

Fig. 3: Module unit, assembled (here right side mounted)

1 Motor unit for horizontal movements (X/Y module)
2 Axial motor unit (A module, without tool mount)
3 A module arc guide

4.2.2 Angle guide

The A module is attached by bolting on the angle guide.
Depending on the mounting point of the angle guide, injection angles between 60° and 30° can
be set.

For the sake of clarity, the A module is not shown.

10

Example:
The angle guide is attached to the X/Y module in the
centre hole, corresponding to 45°. The A module can
be adjusted to between 55° and 35° (45°±10°) by
turning the spindle.

10

1

2

3

68

5

10

10

5

5

4 Installation

The hole spacing is 5°. By moving the mounting point

10

10

5

5

10

5

10

4.2.3 Mounting the axial module (A module)

10

10

of the angle guide 5° upwards, the adjustable angle
changes each time to 50° to 30°.

By moving the mounting point downwards, angles of
up to 60° can be set. This requires sufficient space to
be available on the microscope.

The angle guide is already bolted to the A module.
The spindle may need to be turned until the desired
mounting point (arrow, e.g. here centre hole) is
accessible.

Note

face of the A module must be slackened prior to
adjusting the angle and must then be re-tightened in
order to ensure reliable operation.

4

Installation

: The orange fixing screw on the narrow front

Set the angle guide with the
free hole (2) at the mount (1) of
the X/Y unit. The fitting pins
must be fitted into the holes

1

10

5

5

10

provided. Fix the angle guide
to the X/Y module by the
M4x10 cheese head screw.

2

69

4.2.4 Angle adjuster

4

Installation

4 Installation

10

5

5

10

45°

40°

35°

Set the desired angle.

Mounting the angle guide at the centre hole and
adjusting the A module to 0° corresponds to an angle
of 45°.

To perform the adjustment, the lines on the angle
guide are moved to match the outer edge of the A
module.

Example – 35°: The angle guide is attached by the
centre hole, the bottom edge of the A module is set to
the lower 10° marking.

Note

: The mechanically set angle must be entered
subsequently in the Install menu! Otherwise Z axis
movements cannot be executed correctly! See
section 5.4.2.6.

Note

: The orange fixing screw on the narrow face of
the A module must be slackened prior to adjusting the
angle and must then be re-tightened in order to
ensure reliable operation.

4.2.5 Assembling the hinged bracket

70

Set up the hinged bracket according to the side on

1

which the module unit is to be mounted.
Example of left side mounting:
The hinge (2) is positioned at the left outer, aligned
upwards and to the rear.

2

4 Installation

Opening the hinged bracket

0° – 45°

1

0° – 45°

For right side mounting, the hinge must be at the right
outer (not visible here, on the rear).

The module unit is mounted from above onto the
hinged bracket (1).

4

Installation

To open the hinged bracket beyond its normal
opening angle between 0° and 45° for mounting
purposes, push the pin on the hinge side up. The
slider is purposely hard to move. The unit is usually
only mounted once. The pin can be moved with the 4
mm Allen key.

45° – 90°

The hinged bracket can then be opened to 90°. The
mounting holes on the module are then accessible.

90°

71