Eppendorf InjectMan 4 User Manual

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InjectMan

Operating manual

Eppendorf

and the Eppendorf logo are registered trademarks of Eppendorf AG,

Hamburg, Germany.

InjectMan

4, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of

Eppendorf AG, Hamburg, Germany.

Registered trademarks are not marked in all cases with ™ or ® in this manual.

No part of this publication may be reproduced without the prior permission of the copyright owner.

5192 900.010-00/072013

InjectMan

English (EN)

Operating manualInjectMan® 4seeon p.Fig.Tab.p.

English (EN)Operating manual

Table of contents

1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.1.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.3 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.3.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

2.3.2 Control board. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

2.4 Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

2.5 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.5.1 Dynamic range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

2.5.2 Direction of movement of the joystick. . . . . . . . . . . . . . . . . . . . . . 16

2.5.3 Joystick key functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

2.6 Speed ranges. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

3.3 Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.1.1 If there is any damage, make a claim . . . . . . . . . . . . . . . . . . . . . . 20

4.1.2 Delivery incomplete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.1.3 Microscope adapter assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.3 Mounting overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.3.1 Module (X, Y, Z). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

4.3.2 Z-module holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.3 Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

4.3.4 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

4.3.5 Swivel joint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Table o f co nte nts

InjectMan

English (EN)

4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

4.5 Inserting the capillary holder into the angle head . . . . . . . . . . . . . . . . . . . . 28

4.6 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 29

4.6.1 Inserting Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

4.7 Setting the injection angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

4.8 Aligning the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.8.1 Aligning the height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.8.2 Aligning the depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

4.8.3 Aligning the width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.8.4 Aligning the angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.9 Entering mounting parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.9.1 Microscope and adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

4.9.2 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.9.3 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

4.10 Converting the swivel joint for left side mounting . . . . . . . . . . . . . . . . . . . . 34

4.11 Converting the angle head for left side mounting . . . . . . . . . . . . . . . . . . . . 37

4.12 Connect the motor module to the control board. . . . . . . . . . . . . . . . . . . . . . 38

4.13 Setting installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

4.13.1 First set-up wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

4.14 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

4.14.1 Connecting the FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

4.14.2 Connecting the PiezoXpert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43

4.14.3 Connecting the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

4.14.4 Connecting two devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

5.1 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

5.1.1 Application display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

5.1.2 Display of coordinates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46

5.1.3 Menu display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.2 Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

5.2.1 Application parameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48

5.2.2 Application – Adherent cell injection . . . . . . . . . . . . . . . . . . . . . . 48

5.2.3 Application – ICSI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49

5.2.4 Application – Developmental biology . . . . . . . . . . . . . . . . . . . . . . 49

5.2.5 Application – Physiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.2.6 Application – My application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

5.3 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

5.4 Navigating the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52

5.4.1 Entering or changing parameters . . . . . . . . . . . . . . . . . . . . . . . . . 52

Table of contents

InjectMan

English (EN)

6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1 Switching the device on or off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.1.2 Switching the device off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.2 Activating or deactivating the control board. . . . . . . . . . . . . . . . . . . . . . . . . 53

6.2.1 Activating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.2.2 Deactivating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

6.3 Defining the start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.3.1 Defining the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.3.2 Defining the selected application . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.4 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54

6.4.1 Manually positioning the capillary . . . . . . . . . . . . . . . . . . . . . . . . 55

6.4.2 Automatically positioning the capillary . . . . . . . . . . . . . . . . . . . . . 55

6.5 Changing the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.6 Changing the speed range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56

6.6.1 Changing parameters using the selection dial . . . . . . . . . . . . . . . 56

6.6.2 Changing parameters in the menu . . . . . . . . . . . . . . . . . . . . . . . . 57

6.7 Capillary positions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.7.1 Saving a position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

6.7.2 Moving to the position using the softkey . . . . . . . . . . . . . . . . . . . 58

6.7.3 Moving to the position using the joystick key . . . . . . . . . . . . . . . . 58

6.7.4 Deleting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58

6.7.5 Overwriting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.8 Vertical limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.8.1 Defining the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

6.8.2 Deleting the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.8.3 Defining the upper limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.8.4 Deleting the upper limit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

6.9 Horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.9.1 Defining the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.9.2 Deleting the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

6.10 Speed function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.10.1 Speed menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.10.2 Setting the parameters for Speed . . . . . . . . . . . . . . . . . . . . . . . . . 62

6.11 Inject function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.11.1 Inject menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

6.11.2 Executing the Inject function . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

6.12 Step injection function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65

6.12.1 Step injection menu and parameters. . . . . . . . . . . . . . . . . . . . . . . 65

6.12.2 Executing the Step injection function . . . . . . . . . . . . . . . . . . . . . . 66

Table o f co nte nts

InjectMan

English (EN)

6.13 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.1 Home menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.2 Setting the parameter for Home . . . . . . . . . . . . . . . . . . . . . . . . . . 66

6.13.3 Move the capillary out with the home key . . . . . . . . . . . . . . . . . . 67

6.13.4 Move the capillary back with the home key . . . . . . . . . . . . . . . . . 67

6.13.5 Setting the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67

6.13.6 Terminate the home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14.1 Clean menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

6.14.2 Setting the parameter for Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.14.3 Execute the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.14.4 Terminate the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69

6.15 PiezoXpert function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.15.1 PiezoXpert menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 70

6.15.2 Execute the PiezoXpert function . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16 Installation function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.1 Installation menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.2 Installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.3 Control board parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71

6.16.4 Module parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.17 Function function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.17.1 Function menu and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . 72

6.17.2 Executing Zero coordin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.3 Executing Center motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.17.4 Executing User default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

6.18 Softkeys function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18.1 Softkeys menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . 74

6.18.2 Executing Softkeys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75

6.18.3 Executing Joystick key. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.19 Change appl. function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

6.19.1 Change appl. menu and parameters . . . . . . . . . . . . . . . . . . . . . . . 76

6.19.2 Defining the selected application as the start screen . . . . . . . . . . 76

6.19.3 Defining the application as the start screen . . . . . . . . . . . . . . . . . 77

6.20 Service function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6.20.1 Service menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77

6.20.2 Execute the Selftest function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21 Resetting parameters to the factory settings . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21.1 Perform reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.21.2 Carrying out a reset in the menu . . . . . . . . . . . . . . . . . . . . . . . . . . 78

6.22 Remote computer control of the InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . 78

Table of contents

InjectMan

English (EN)

7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.2 Capillary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.3 Control board and display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79

7.1.4 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.1.5 Software and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2.1 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80

7.2.2 Error. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81

8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.1 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

8.3 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

8.4 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83

9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

9.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85

10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86

10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87

11 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.1 InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.2 Accessories for InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88

11.3 Tools for InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.4 Microscope adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89

11.5 Accessories for adapters for microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.6 Capillaries for research applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90

11.7 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91

11.8 Universal capillary holder and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . 91

11.9 CellTram and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

11.10 FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92

11.11 FemtoJet express. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93

11.12 Accessories for FemtoJet/FemtoJet express . . . . . . . . . . . . . . . . . . . . . . . . . 93

11.13 PiezoXpert. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95

Operating instructions

InjectMan

English (EN)

1 Operating instructions

1.1 Using this manual

 Read this operating manual completely before using the device for the first time.

Please also note the operating instructions for the accessories, if applicable.

 This operating manual is part of the product. Thus, it must always be easily accessible.  Enclose this operating manual when transferring the device to third parties.  If this manual is lost, please request another one. You will find the current version on

our webpage www.eppendorf.com/worldwide

1.2 Danger symbols and danger levels

The safety instructions in this manual appear with the following danger symbols and danger levels:

1.2.1 Danger symbols

Hazard point Cuts

Material damage Electric shock

Biohazard

1.2.2 Danger levels

DANGER Will lead to severe injuries or death.

WAR NIN G Can lead to severe injuries or death.

CAUTION Can lead to light to moderate injuries.

ATTENTION May lead to material damage./Paragraph

1.3 Symbols used

Depiction Meaning

Actions in the specified order

 Actions without a specified order

• List:

Text Display or software texts

Additional information

Product description

2 Product description

2.1 Delivery package

Quantity Description

1X-module

1Y-module

1Z-module

1 YZ-connector

1 Swivel joint

1 Angle head

1 Control board

1Mains/power cord

1Cable sheathing

1 Operating manual

2.1.1 Tools

Quantity Description

7 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm Allen key

1 3 mm Allen torque key

1 1.3 mm Allen key

1 Tool bag

InjectMan

English (EN)

2.1.2 Accessories

Quantity Description

1 Connecting cable for FemtoJet/FemtoJet express

2 Positioning aids for capillary holders

1 Spare parts kit

1Label

2.2 Features

The micromanipulator InjectMan 4 has been especially developed for work processes that require intuitive movement of the capillary.

The InjectMan 4 combines the classical benefits of a mechanical system with the benefits of an accurate electrically driven system.

Product description

InjectMan

English (EN)

The capillary is controlled by a joystick. A greater forwards or backwards movement of the joystick results in an acceleration of the capillary movement. The movement ranges enable moving to any position in the working range of the micromanipulator.

The dynamic movement of the joystick is especially suitable for serial injections and working techniques that require fast injection movement.

The software control provides predefined applications, freely programmable softkey functions, a freely programmable application and the storage of different positions all space coordinates.

2.3 Main illustration

The motor module is mounted on a special microscope adapter or on a free-standing stand (magnetic). The control board is mechanically separated from the motor module.

Abb. 2-1:InjectMan 4 — right side mounting

Fig. 2-1: InjectMan 4 — right side mounting

1 Motor module 2 Control board

Product description

InjectMan

English (EN)

2.3.1 Motor module

The motor module is made up of three modules. The capillary can be moved in all three spatial axes due to the layout of the modules. The X-module with the capillary can be swung out of the working range using the swivel joint. The injection angle of the capillary can be set to any angle on the angle head.

Abb. 2-2:Motor module

Fig. 2-2: Motor module

1 Z-module

2 YZ-connector

3 Angle head

4 Y-module

5 Swivel joint

6 X-module

Product description

InjectMan

English (EN)

2.3.2 Control board

The control board contains the key pad, the display and the joystick, and, to the side, the selection dial. The direction of movement and the speed of the joystick are transferred to the capillary. The responsitivity of the movement and the size of the working range are predefined in the software setting. On the control panel, the working range can be selected and modified via the selection dial.

Abb. 2-3:Control board - front

Fig. 2-3: Control board - front

1 Joystick

Dynamic movement

2 Display

coarse

fine

home

3 Control panel

4 Selection dial

For increasing or reducing the speed

Abb. 2-4:Control board - rear

Product description

InjectMan

English (EN)

Fig. 2-4: Control board - rear

1 Mains power switch On/Off

2 Connection for an external device

Foot control, FemtoJet, FemtoJet express, PiezoXpert or PC

3 Connection for a Y-module

4 Connection for an X-module

ext. Device

Z-Axis

Y-Axis

X-Axis

SERVICE

5 Connection for a Z-module

6 Service interface

7 Mains/power connection

8 Fuse

Product description

InjectMan

English (EN)

2.4 Control panel

With the keys on the control panel you can switch on the control board and select the size of the working range. The softkeys are used to open applications, execute functions, navigate the menu and set parameters.

Abb. 2-5:Control panel

Fig. 2-5: Control panel

1 home key

Moves the capillary out of the working range into a defined position

2 Display

Displays the software

3 standby key

Switches the control board on or off or cancels automatic movements

4 Softkeys 1 – 5

For selecting the application, triggering the function, navigating or setting parameter values

fine

x–fine

homecoarse

5 menu key

Opens the menu

6 fine/x-fine key

For setting the medium or low speed range

7 coarse key

For setting the high speed range

Product description

InjectMan

English (EN)

2.5 Joystick

The joystick controls the capillary in all three spatial axes. The movement of the capillary is accelerated the further the joystick is moved forwards or backwards.

Abb. 2-6:Joystick

Fig. 2-6: Joystick

1 Joystick key 2 Joystick

Controls movements in the X, Y and Z-axis

2.5.1 Dynamic range

The size of the dynamic range is limited by the movement range of the modules (X, Y and Z).

When the joystick is moved, the capillary starts moving into the direction of the joystick movement. The movement of the capillary is accelerated the further the joystick is moved. The movement stops when the joystick is released.

Product description

InjectMan

English (EN)

2.5.2 Direction of movement of the joystick

The joystick can be moved along the horizontal plane. This controls the motors of the X-module and the Y-module. The joystick can be moved in one axis at a time or in a combination of axes. Rotating the joystick moves the motor module on the vertical axes.

Abb. 2-7:Movement in the X and Y-axis

 Move the capillary in the horizontal

direction (X and Y-axis).

Fig. 2-7: Movement in the X and Y-axis

Abb. 2-8:Movement in the Z-axis

Fig. 2-8: Movement in the Z-axis

2.5.3 Joystick key functions

Abb. 2-9:Function

Fig. 2-9: Function

 Move the capillary in the vertical

direction (Z-axis).

 Trigger function (e.g., trigger injection).

Product description

InjectMan

English (EN)

2.6 Speed ranges

There are three speed ranges in the movement range of the joystick. The speed can be set using the selection dial on the control board and in the Speed menu.

Speed ranges:

• coarse – for a large working range

• fine – for a medium working range

• x-fine – for a small working range

Safety

InjectMan

English (EN)

3Safety

3.1 Intended use

The InjectMan 4 has been designed and manufactured for use in biological, chemical and physical research. It is used for the precise positioning of microcapillaries and similar tools as well as for the transfer of very small sample volumes.

The InjectMan 4 has been designed and manufactured for research use only.

The InjectMan 4 is intended exclusively for indoor use and for operation by qualified staff.

3.2 Warnings for intended use

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

CAUTION! Risk of cuts from broken capillaries.

Capillaries are made of glass and are very fragile.

 Wear your personal protective equipment (PPE).  Always mount capillaries depressurized.  Never aim capillaries at people.  Handle the capillaries very carefully.

NOTICE! Mechanical damage to the motor modules.

Excessive load leads to increment errors or destruction of the drive.

 Do not drive the modules against mechanical obstructions.  Do not hold any objects near the modules.  Load the motor module with a maximum of 100 g.

WARNING! Damages to health due to infectious liquids and pathogenic germs.

 When handling infectious liquids and pathogenic germs, observe the national

regulations, the biological security level of your laboratory, the material safety data sheets, and the manufacturer's application notes.

 Wear personal protective equipment.  Consult the "Laboratory Biosafety Manual" (Source: World Health

Organization, Laboratory Biosafety Manual, as amended) for comprehensive regulations on the handling of risk group II germs or biological materials.

3.3 Warning signs on the device

Warning symbol Meaning

Warns of the risk of injury caused by capillary tips

Warns of the danger of crushing on the motor module

Safety

InjectMan

English (EN)

Read the operating manual

3.4 Information on product liability

In the following cases, the designated protection of the device may be compromised. Liability for any resulting property damage or personal injury is then transferred to the operator:

• The device is not used in accordance with the operating manual.

• The device is used outside of its intended use.

• The device is used with accessories or consumables which are not recommended by Eppendorf.

• The device is maintained or repaired by people not authorized by Eppendorf.

• The user makes unauthorized changes to the device.

Installation

InjectMan

English (EN)

4 Installation

4.1 Preparing installation

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Keep the packaging and the transport securing devices for later transport or storage.

Do not operate the device if there is visible damage to the device itself and/or to its packaging.

1. Check the packaging for damage.

2. Carefully remove the motor module and the control board from the packaging.

3. Check that everything is included in the delivery.

4. Check the modules, the control board and the accessories for damage.

4.1.1 If there is any damage, make a claim

1. Contact customer service.

4.1.2 Delivery incomplete

1. Contact customer service.

4.1.3 Microscope adapter assembly

The microscope adapter is not included in the scope of delivery and must be ordered separately.

1. Assemble the microscope adapter in accordance with the assembly instructions for the microscope adapter.

4.2 Selecting the location

Select the location for the device according to the following criteria:

• Mains connection in accordance with the name plate.

• A bench with a horizontal and even work surface which is designed to support the weight of the device.

• A mat or table that is cushioned against vibrations.

• The location is protected from direct sunlight and drafts.

The mains/power switch and cutting unit of the mains/power line must be easily accessible during operation (e.g, residual current circuit breaker).

4.3 Mounting overview

Abb. 4-1:Overview for right-hand side mounting

Installation

InjectMan

English (EN)

Fig. 4-1: Overview for right-hand side mounting

1 Z-module holder

For left side mounting

2 Microscope adapter

3 Z-module holder

For right side mounting

4 Z-module

5 Y-module

6 Swivel joint

7 X-module

8 Universal capillary holder

(not included in the delivery package)

9 Angle head

10 YZ-connector

5 6

8910

Installation

InjectMan

English (EN)

4.3.1 Module (X, Y, Z)

Abb. 4-2:Y-module

Fig. 4-2: Y-module

1 Cable

2 Module identification

3 Movable rail

4 Scale

Movement range of the rail

5 Fixed rail

4.3.2 Z-module holder

Abb. 4-3:Z-module holder, front and back

Installation

InjectMan

English (EN)

Fig. 4-3: Z-module holder, front and back

1 Slider

2 Screw

Attach Z-module

4.3.3 Slider

Abb. 4-4:Slider

3 Screw

Attach Z-module holder to the adapter

4 Stop angle

Fig. 4-4: Slider

1 Slider

2 Lock washer

3 Flat washer

4 Screw

Installation

InjectMan

English (EN)

4.3.4 Angle head

Abb. 4-5:Angle head

Fig. 4-5: Angle head

1 Identification

For setting the angle

2 Knurled screw

For setting the injection angle

3 Universal capillary holder

(not included in the delivery package)

4 Positioning aid

5 Knurled screw

For fixing the universal capillary holder

4.3.5 Swivel joint

Abb. 4-6:Swivel joint for right side mounting

Installation

InjectMan

English (EN)

6 7

Fig. 4-6: Swivel joint for right side mounting

1 Slider

Y-module

2 Turntable

3 Allen screws

4 Slider

X-module

5 Stop plate

6 Upper joint

7 Mounting mark

| stands for left side mounting || stands for right side mounting

8 Lower joint

4.4 Mounting the motor module

The motor module can be mounted on the right-hand or left-hand side of the microscope adapter. The following describes mounting on the right-hand side. For mounting on the left-hand side, the swivel joint and the angle head must be modified.

The motor module is mounted as standard on an inverse microscope. It can also be mounted on a universal stand. Mounting on a universal stand is described in the corresponding manual.

Installation

InjectMan

English (EN)

4.4.1 Mounting the Z-module

Prerequisites

• 3 mm Allen torque key

1. Push the Z-module holder onto the microscope adapter and tighten.

2. Push the fixed rail onto the Z-module holder until it reaches the stop angle and tighten the screw. The connecting cable faces to the rear.

4.4.2 Mounting the Y-module

Prerequisites

• 3 mm Allen torque key

Installation

InjectMan

English (EN)

1. Push the YZ-connector onto the fixed rail of the Y-module and tighten.

2. Push the Y-module with the YZ-connector onto the Z-module and tighten.

4.4.3 Mounting the X-module

Prerequisites

• 3 mm Allen torque key

1. Push the swivel joint onto the fixed rail of the X-module and tighten.

Installation

InjectMan

English (EN)

4.4.4 Mounting the angle head

Prerequisites

• 3 mm Allen torque key

2. Push the swivel joint with the X-module onto the Y-module and tighten.

1. Push the angle head onto the X-module.

2. Slightly tighten the angle head.

4.5 Inserting the capillary holder into the angle head

Prerequisites

• A universal capillary holder from Eppendorf is available.

• A capillary holder (4 mm diameter) from a different manufacturer is available.

1. Loosen the knurled screw on the angle head.

2. Insert the capillary holder into the clamp.

3. Align the capillary holder in such a way that the capillary tip is located approx. 20 mm above and approx. 20 mm from the outside of the operating point.

4. Place the positioning aid on the capillary holder and tighten.

5. Tighten the knurled screw.

4.6 Inserting the capillary into the capillary holder

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

Installation

InjectMan

English (EN)

NOTICE! Mechanical damage to the motor modules.

Excessive load leads to increment errors or destruction of the drive.

 Do not drive the modules against mechanical obstructions.  Do not hold any objects near the modules.  Load the motor module with a maximum of 100 g.

Standard capillary: Only use the 0 grip head with capillaries with an outer diameter of 1.0 mm to 1.1 mm. If you would like to use other capillaries, order the matching grip head.

Installation

InjectMan

English (EN)

1. Push the capillary into the grip head until it reaches the stop and tighten the grip head.

4.6.1 Inserting Femtotips

1. Remove the grip head.

2. Screw the adapter for Femtotips into the universal capillary holder.

3. Screw the Femtotip into the adapter and tighten.

4.7 Setting the injection angle

1. Turn the knurled screw to set the injection angle.

Installation

InjectMan

English (EN)

4.8 Aligning the motor module

Centrally align the modules in order to be able to use the full movement range of the modules.

4.8.1 Aligning the height

1. Undo the screw on the YZ-connector.

2. Align the Y-module on the scale of the Z-module and tighten the screw.

4.8.2 Aligning the depth

1. Undo the screw on the swivel joint.

2. Align the X-module on the scale of the Y-module and tighten the screw.

Installation

InjectMan

English (EN)

4.8.3 Aligning the width

4.8.4 Aligning the angle head

1. Undo the screw on the Z-module holder.

2. Align the Z-module on the scale of the microscope adapter and tighten the screw.

1. Undo the screw on the angle head.

2. Align the angle head on the scale of the X-module.

4.9 Entering mounting parameters

To facilitate remounting the mounting parameters can be recorded.

1. Enter the mounting parameters in the tables.

4.9.1 Microscope and adapter

Name Type

Microscope

Adapter

Attachment side of the motor module

4.9.2 Motor module

Position Description Position [mm]

1 Z-module holder on the adapter

2 Z-module on the Z-module holder

3 YZ-connector on the Z-module

4 YZ-connector on the Y-module

5 Swivel joint on the Y-module

6 Swivel joint on the X-module

7 Angle head on the X-module

3 4

Installation

InjectMan

English (EN)

4.9.3 Angle head

Name Position [mm] Degrees

Capillary holder

Injection angle

Installation

InjectMan

English (EN)

4.10 Converting the swivel joint for left side mounting

Prerequisites

• 2 mm Allen key

• Right side mounting marks (||) are aligned above each other

1. Rotate the lower joint until both Allen screws are accessible.

2. Unscrew the Allen screws.

InjectMan

English (EN)

3. Remove the stop plate.

4. Rotate the lower joint back.

5. Rotate the upper joint by 180°. The sliders must be at a 90° angle to each other.

Installation

InjectMan

English (EN)

6. Align the left side mounting marks (I).

7. Fit the stop plate in such a way that the pins sit in the holes of the turntable.

8. Rotate the swivel joint by 180°.

9. Screw down the stop plate.

• Left side mounting marks (|) are aligned above each other.

10.Check the position of the joints.

• The sliders must be at a 90° angle to each other.

90°

4.11 Converting the angle head for left side mounting

Prerequisites

• 1.3 mm Allen key

1. Undo the set screw and pull the knurled screw off the spindle.

Installation

InjectMan

English (EN)

Installation

InjectMan

English (EN)

2. Rotate the angle head by 180°.

3. Push the knurled screw onto the spindle end and fasten it with the set screw.

4. Turn the knurled screw until the desired

angle is set.

4.12 Connect the motor module to the control board

WARNING! Risk from incorrect supply voltage

 Only connect the device to power supplies which correspond with the

electrical requirements on the name plate.

 Only use sockets with a protective earth (PE) conductor and suitable power

cable.

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Installation

InjectMan

English (EN)

NOTICE! Material damage from incorrect connections.

 Only electrical connections may be made to devices described in the

operating manual.

 Other connections are permitted only following consultation and agreement

with Eppendorf AG.

 Only connect devices that meet the safety requirements defined in

IEC 60950-1.

NOTICE! Short circuit caused by incorrect installation.

 Failure to observe the order of steps may result in a short circuit.

Prerequisites

• InjectMan 4 is switched off.

• The power cable is disconnected.

1. Connect the module (X,Y,Z) plug with the ports on the control board.

2. Tighten the fixing screws on the plug manually.

3. Connect the power cable.

4. Switch on the mains switch.

5. Set the installation parameters. You can use the software wizard First set-up or the

Installation menu to set the Side and Angle parameters.

4.13 Setting installation parameters

Installation parameters must be set:

• for the first set-up

•after a reset

The following settings are defined:

• Installation side of the motor module

• Operating angle for the capillary

• Center motors

• Adjust motors

•Set date

Installation

InjectMan

English (EN)

4.13.1 First set-up wizard

Prerequisites

• The micromanipulator is switched on.

1. Select the First set-up application.

2. Select the installation side.

3. Select Next.

4. Set the operating angle.

5. Select Next.

Installation

InjectMan

English (EN)

6. Select the Execute function to move all motors to the middle position.

7. Select Next.

8. Insert the capillary holder into the angle head.

9. Select Next.

10.Manually align the modules.

11.Select Next.

12.Remove the capillary holder.

13.Insert the capillary into the capillary holder.

14.Insert the capillary holder with the capillary into the angle head.

15.Finely adjust the position of the capillary holder and the modules.

16.Select Next.

Installation

InjectMan

English (EN)

17.Set the time and the date.

18.Select Next.

19.Select Enter.

• The installation is complete and the modules are adjusted.

• The application screen displays My application

Installation

InjectMan

English (EN)

4.14 Connecting an external device

The following devices can be connected to the control board:

• Eppendorf FemtoJet

• Eppendorf PiezoXpert

•Computer

4.14.1 Connecting the FemtoJet

Prerequisites

• Devices are switched off.

The operation is described in the manual for the FemtoJet.

1. Connect the FemtoJet to the port for external devices.

2. Switch on the FemtoJet. The initialization phase starts.

3. Switch on the control board.

• After completion of the initialization phase, the status message Injector ready appears on the application screen.

4.14.2 Connecting the PiezoXpert

Prerequisites

• Devices are switched off.

The operation is described in the manual for the PiezoXpert.

1. Connect the PiezoXpert to the port for external devices.

2. Switch on the PiezoXpert. The initialization phase starts.

3. Switch on the control board.

• After completion of the initialization phase, the status message PiezoXpert ready appears on the application screen.

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4.14.3 Connecting the Computer

Prerequisites

• A data cable is available.

• Devices are switched off.

Control with a computer is described in the Cell Technology · PC Control manual.

1. Connect the data cable to the port for external devices.

2. Connect the computer to the data cable.

3. Switch on the control board.

4.14.4 Connecting two devices

Prerequisites

• Y-connector is available.

• Devices are switched off.

Two devices can be connected with a Y-connector.

The following combinations are possible:

• Computer and FemtoJet

• FemtoJet and PiezoXpert

1. Connect the Y-connector to the port for external devices.

2. Connect the device combination.

3. Connect the devices.

• After initialization, status messages appear on the application screen.

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5 Software

5.1 Display

The display shows current settings, for example, the selected working range, the position of the motors and defined limits.

5.1.1 Application display

Abb. 5-1:Display layout - ICSI application as an example

Fig. 5-1: Display layout - ICSI application as an example

1 Status line with working range

2 Active application with speed bars

3 Connected device

4 Status fields of the softkeys

5 Softkeys

6 Function of the joystick key

7 Display of coordinates

8 Display of the defined limits

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5.1.2 Display of coordinates

Abb. 5-2:Display of coordinates

Fig. 5-2: Display of coordinates

1 2

1 Motor limit stop

+ = positive, - = negative

2 Axis is deactivated

3 Lower limit (Z-axis Limit) reached

4 Display of the lower limit

5 Lower limit activated

5.1.3 Menu display

Abb. 5-3:Menu and parameter display

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Fig. 5-3: Menu and parameter display

1 Navigation path

2 Menu

3 Arrow up/down softkey

For navigation and changing parameters.

5.2 Applications

Abb. 5-4:Application screen

4 Enter softkey

For confirming input, executing the function, saving parameters

5 Arrow left/right softkey

Navigation

6 Parameter

Fig. 5-4: Application screen

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Application selection

• Select the application

• Store the main application

5.2.1 Application parameter

Predefined functions of the different applications.

• Pos 1 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key.

• Pos 2 – Save the X, Y and Z values of the capillary position. Switch the position using the joystick key.

• Activate or deactivate the Step inject function.

• Y off – Switch off the movement of the capillary in the Y-axis. Prevents moving sideways during injection.

• Z-axis Limit – Save the downward limit for the vertical capillary movement.

• Axial – Switch on the capillary movement along the assembly bracket. Suitable for straight capillaries.

• Reduce or increase the Limit value for the limit.

•Execute the Clean function.

5.2.2 Application – Adherent cell injection

This application is suitable for injection into adherent cells.

Abb. 5-5:Application 1 Adherent cell injection

Fig. 5-5: Application 1 Adherent cell injection

Parameter selection

• Joystick key – Inject

• Freely program the softkey

• Reduce the value for Z-axis Limit

• Set the lower limit (Z-axis Limit)

• Increase the value for Z-axis Limit

• Execute the function

5.2.3 Application – ICSI

This application is suitable for intracytoplasmic sperm injection.

Abb. 5-6:Application 2 ICSI

Fig. 5-6: Application 2 ICSI

Parameter selection

• Joystick key – Switch position

• Save positions 1 and 2

• Freely program the softkey

• Deactivate the control of the axis of movement (Y-axis)

• Set the lower limit (Z-axis Limit)

5.2.4 Application – Developmental biology

This application is suitable for developmental biology.

Abb. 5-7:Application 3 Developmental biology

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Fig. 5-7: Application 3 Developmental biology

Parameter selection

• Joystick key – Inject

• Save position 1

• Activate the Step inject function

• Switch on the axial movement of the Z-axis

• Freely program the softkey

• Set the lower limit (Z-axis Limit)

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5.2.5 Application – Physiology

This application is suitable for physiology.

Abb. 5-8:Application 4 Physiology

Fig. 5-8: Application 4 Physiology

Parameter selection

• Joystick key – Joystick off

• Save position 1

• Freely program the softkey

• Switch on the axial movement of the Z-axis

• Deactivate the control of the axes of movement (X and Y-axis)

• Set the lower limit (Z-axis Limit)

5.2.6 Application – My application

No softkeys are preprogrammed for this application. This application can be individually programmed.

Abb. 5-9:Application 5 My application

Fig. 5-9: Application 5 My application

Parameter selection

• Freely program the joystick key

• Freely program all softkeys

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5.3 Main menu

Abb. 5-10:Main menu

Fig. 5-10: Main menu

Menu Parameter

Speed Set the speed parameters

Inject Set the injection parameters

Step injection Set the injection parameters

Home Set the parameters for the Home movement

Clean Set the parameters for the Clean movement

PiezoXpert Set the parameters for an optional device

Installation Set the device parameters

Function Execute the device function

Softkeys Program softkeys

Change appl. Change the selected application or activate the application screen

Service Execute the service function on a user basis

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5.4 Navigating the menu

Abb. 5-11:Software navigation

Fig. 5-11: Software navigation

Navigate the menu using the arrow keys. Pressing Enter will confirm the selection. You can switch between menus and submenus with the arrow keys to the left and to the right.

5.4.1 Entering or changing parameters

Parameters can be changed in the menu using the arrow keys, the selection dial or the rotating wheel on the joystick.

Abb. 5-12:Changing the parameters

 Change values with the upwards or

downwards arrow keys.

 Change values using the selection dial.  Change values using the upper part of

the joystick.

 Save with Enter.

Fig. 5-12: Changing the parameters

6Operation

WARNING! Electric shock due to damage to device or mains cable.

 Only switch on the device if the device and mains cable are undamaged.  Only use devices that have been properly installed or repaired.  In case of danger, disconnect the device from the mains supply by pulling the

power plug from the device or the mains socket or, by using the isolating device intended for this purpose (e.g., emergency stop switch in the laboratory).

Do not move the joystick immediately after power-on. Wait until the initialization is completed. The completion of initialization is indicated by the display switching to the operating state.

6.1 Switching the device on or off

6.1.1 Switching on the device

1. Switch on the device at the mains power switch.

• The motor module and the control board are switched on.

• The device runs through an initialization phase.

• The application screen will appear.

6.1.2 Switching the device off

1. Switch off the device at the mains power switch.

The motor module and the control board are deenergized.

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6.2 Activating or deactivating the control board

6.2.1 Activating the control board

Prerequisites

• The display shows STANDBY.

1. Press the standby key.

• The keys, joystick, selection dial and softkeys are activated.

• The display shows the application screen.

6.2.2 Deactivating the control board

The step m otors slowly move to the ne xt parking position. This prevents the motors falling back to the parking position and the capillary jumping.

1. Press the standby key.

• The keys, joystick and selection dial are deactivated

• The display shows STANDBY.

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• Current movements are stopped.

• The motor module remains switched on so that the step motors keep their current position.

6.3 Defining the start screen

An application can be selected as the default application. The micromanipulator then starts with the defined application. The application screen with all the applications can be redefined in the Start display menu.

6.3.1 Defining the application

1. Press and hold the softkey of the desired application for 3 seconds.

• The micromanipulator starts with the defined application.

6.3.2 Defining the selected application

1. In the Change appl. menu, select the Start display submenu.

2. Confirm with Execute.

3. Close the menu.

• The micromanipulator starts with the selected application.

6.4 Replacing the capillary

Prerequisites

• The capillary is depressurized.

WARNING! Risk of injury due to flying capillaries and glass splinters.

If exposed to high pressures, capillaries may detach themselves from the grip heads and become projectiles. Capillaries can crack as a result of incorrect handling.

 Wear protective goggles.  Never aim capillaries at people.  Use capillaries with an outer diameter that matches the grip head

specifications.

 Always mount / dismount capillaries when they are depressurized.  Mount the capillary correctly in the grip head.  Do not touch the capillary with the Petri dish or other objects.

CAUTION! Risk of injury from capillaries

Capillaries can easily penetrate your skin.

 After changing a capillary, swivel it immediately back to the working range.

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CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Move the capillary out of the working range using the home key.

2. Swing the X-module forwards.

3. Undo the grip head on the capillary holder.

4. Carefully pull the capillary out of the grip head.

5. Push the new capillary into the grip head until it reaches the stop and tighten the grip head.

6. Swing the X-module back.

6.4.1 Manually positioning the capillary

Suitable when using capillaries of different lengths (e.g., self-pulled capillary).

1. Press Back manual.

2. Manually position the capillary in the working range.

6.4.2 Automatically positioning the capillary

Suitable when using industrial capillaries (e.g., Femtotips).

1. Press home.

• The capillary automatically moves back into the working range.

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6.5 Changing the sample

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Press the home key to move the capillary

2. Swing the X-module backwards.

3. Change the sample.

4. Swing the X-module back.

5. Press the home key to move the capillary

6.6 Changing the speed range

6.6.1 Changing parameters using the selection dial

out of the working range.

back into the working range.

coarse

1. Press the key for the desired speed range on the control panel.

2. Turn the selection dial to change the

fine

speed.

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6.6.2 Changing parameters in the menu

Prerequisites

• An application has been selected.

1. Press the menu key.

2. Select theSpeed menu.

3. Select the desired parameter.

4. Change the value.

6.7 Capillary positions

To secure the capillary when moving a slide and to move it back to the same work position you can store the positions. Depending on the application, a maximum of five positions can be stored.

As soon as the capillary leaves a stored position, the filled circle is displayed as an empty circle, to show that this position is now stored. If no positions are stored, the status field will be empty.

6.7.1 Saving a position

Prerequisites

• An application has been selected.

1. Move the capillary to the desired position.

2. Hold the Pos 1 softkey for approx. one second to store the working position of the capillary.

• An acoustic signal will sound.

• Pos 1 is marked.

• The stored position is displayed in the status field.

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3. Move the capillary to the desired position (e.g., parking position).

4. Press and hold the Pos 2 softkey for approx. one second to store the parking position of the capillary.

• An acoustic signal will sound.

• Pos 2 is marked.

• The stored position is displayed in the status field.

6.7.2 Moving to the position using the softkey

Prerequisites

• At least one position is stored.

1. Press a softkey with a stored position.

• You will move to the selected position.

• The joystick is deactivated until the position has been reached.

• The LEDs of the softkeys flash.

• In the softkey status field a filled circle is displayed.

If a stored position is lower than the lower limit (Z-axis Limit), the position on the Z-axis will only be approached up to the defined limit.

6.7.3 Moving to the position using the joystick key

Prerequisites

• At least one position is stored.

•The Joystick key parameter must be set to the Switch position value.

 Press the joystick key.

You will move to the first position

 Press the joystick key.

• You will move to the next position.

6.7.4 Deleting a stored position

Prerequisites

• A position is stored.

1. When the position has been reached, hold the softkey.

• An acoustic signal will sound.

• The position has been deleted.

• The status field is empty

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6.7.5 Overwriting a stored position

Prerequisites

• A position is stored.

1. Press the softkey for a different position.

• You will move to the position.

2. When the position has been reached, press and hold the softkey for the position to be overwritten.

• The old position is overwritten with the current coordinates.

3. Press the softkey.

• An acoustic signal will sound.

• In the softkey status field a filled circle is displayed.

• The stored position is displayed in the coordinate field.

6.8 Vertical limits

For the Z-axis, a lower and upper limit can be defined. This prevents the capillary coming into contact with the bottom of the Petri dish or moving against the condenser of the microscope adapter.

•Lower limit – Z-axis Limit

• Upper limit – Upper limit

6.8.1 Defining the lower limit

Prerequisites

• An application has been selected.

1. Position the capillary a little way above the slide.

2. Press Z-axis Limit.

• The Z-coordinate is marked with L.

• The value for the limit (Limit Z) is displayed.

• Z-axis Limit is selected.

• The capillary cannot be moved any lower.

Upper Limit

Menu / Installation / Upper Limit

Back

Set

Clear

X Y Z

Lim

= = =

0 0

2671

µm µm µm U

µm

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6.8.2 Deleting the lower limit

1. Press Z-axis Limit.

• The limit is deleted.

6.8.3 Defining the upper limit

1. In the Installation menu, select the Upper limit submenu. The Upper Limit window appears.

2. Move the capillary to the top position.

3. Save the position with Set.

• The Z-coordinate is marked with U.

• The value for the limit (Lim) is displayed.

• The capillary cannot be moved any higher.

6.8.4 Deleting the upper limit

1. In the Installation menu, select the Upper limit submenu. The Upper Limit window appears.

2. Save the position with Clear.

• The limit is deleted.

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6.9 Horizontal limit

For the X-axis, a limit can be defined for a horizontal injection. This prevents the capillary from moving through the sample.

6.9.1 Defining the horizontal limit

1. In the Installation menu, select the Angle submenu.

2. Set the injection angle to 0° and save with Enter.

3. Close the menu. The application screen now displays X-axis Limit.

4. Move the capillary to the desired final position for the X-axis.

5. Save the lateral limit with X-axis Limit.

• The X-coordinate is marked with L.

• The value for the limit (Limit X) is displayed.

• X-axis Limit is selected.

• The capillary cannot be moved any further to the side.

6.9.2 Deleting the horizontal limit

1. Press X-axis Limit. The limit is deactivated.

2. In the Installation menu, select the Angle submenu.

3. Reset the injection angle to the operating angle and save with Enter.

• The lateral limit is deleted.

• The application screen displays Back again.

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6.10 Speed function

In the Speed menu you can set the speed of the working range and the speed at which certain positions are approached or movements executed.

6.10.1 Speed menu and parameters

Abb. 6-1:Menu – Speed

Fig. 6-1: Menu – Speed

Parameter Value Range of values Increment Standard

Coarse Set the speed in μm per

5 – 10000 5 7500

second

Fine Set the speed in μm per

5 – 1000 5 1000

second

X-fine Set the speed in μm per

0 – 100 1 100

second

Injection speed Set the speed in μm per

5 – 10000 5 300

second

Step inj. speed Set the speed in μm per

5 – 10000 5 300

second

Position speed Set the speed in μm per

5 – 10000 5 1500

second

Home speed Set the speed in μm per

5 – 10000 5 7500

second

6.10.2 Setting the parameters for Speed

If a value of 0 is set for the X-fine parameter, then the change between the Fine and X-fine working ranges is deactivated.

1. Select the desired parameter.

2. Set the value for the parameter.

3. Close the menu.

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6.11 Inject function

This function performs an automatic injection movement. You can set the injection speed, the injection time, the injection movement and fix the distance between the capillary and the lower limit.

6.11.1 Inject menu and parameters

Abb. 6-2:Menu – Inject

Fig. 6-2: Menu – Inject

Parameter Value Range of values Increment Standard

Injection speed Set the injection speed in μm

5 – 10000 5 300

per second

Synchr. inject. Set the synchronized time of

injection

MOVE IMMEDIATE

– LIMIT

LIMIT PRESSURE

Search+limit Change the lower limit and

OFF/ON – OFF the position of the capillary. The distance between the capillary and the limit remains constant

Injection axial Switch the axial movement of

OFF/ON – On the function on or off

Parameter Range of values Function

Synchr. inject. MOVE Injection movement without injection pressure (when

using non-connected injectors)

IMMEDIATE Injection pressure starts with the injection movement

LIMIT Injection pressure at the end of the injection distance

(Z-axis Limit)

PRESSURE Injection pressure without injection movement

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6.11.2 Executing the Inject function

Prerequisites

• A FemtoJet is connected.

• A lower limit (Z-axis Limit) has been defined.

7 8

1 Position the capillary

2 Trigger an automatic injection

3 Injection

4 Automatic movement to the starting

position

If the Search+limit parameter is activated you can inject cells with different heights using the same injection parameters.

1. Press the joystick key.

• The capillary is moved to the side and to the lower limit at the injection angle.

• The injection is carried out automatically.

• The capillary is moved back to the starting position.

5 Capillary

6 Search level

7 Z-axis Limit

8 Cell

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6.12 Step injection function

With this function you can execute a straight injection over a defined distance. Step

injection can only be executed when a FemtoJet is connected.

6.12.1 Step injection menu and parameters

Abb. 6-3:Menu – Step injection

Fig. 6-3: Menu – Step injection

Parameter Value Range of values Increment Standard

Step injection Switch the function on or off OFF/ON – OFF

Step inj. speed Set the injection speed in μm

5 – 10000 5 300 per second

Step inj. dist. Set the injection path in μm 1 – 2000 1 20

Step inj. axial Switch the axial movement of

OFF/ON – On the capillary on or off

Step inj. back Switch the backward

OFF/ON – On movement of the capillary on or off

Synchr. inject. Set the time of injection MOVE

– LIMIT

IMMEDIATE

LIMIT

PRESSURE

Parameter Range of values Function

Synchr. inject. MOVE Injection movement without injection pressure

IMMEDIATE Injection pressure starts with the injection movement

LIMIT Injection pressure at the end of the injection distance

(Step inj. dist.)

PRESSURE Injection pressure without injection movement

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6.12.2 Executing the Step injection function

Prerequisites

• A FemtoJet is connected.

• Step injection is assigned to a free softkey.

1. Activate Step injection.

2. Set all parameters.

3. Set the injection time on the FemtoJet.

4. Close the menu.

• The application screen displays Step injection.

5. Trigger the injection with the joystick key.

• The function is executed.

6.13 Home function

The Home function quickly moves the capillary out of the work area and is suitable for a quick change of capillary.

6.13.1 Home menu and parameters

Abb. 6-4:Menu – Home

Fig. 6-4: Menu – Home

Parameter Value Range of values Increment Standard

Home speed Set the speed of the Home

5 – 10000 5 7500 function

Home offset Set vertical offset in μm 5 – 20000 5 0

6.13.2 Setting the parameter for Home

1. Set the speed.

2. Set the offset.

3. Close the menu.

6.13.3 Move the capillary out with the home key

Prerequisites

• The parameters in the Home menu have been defined.

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Press the home key. The home key flashes.

• The capillary is moved out of the work area.

•The home key lights up.

6.13.4 Move the capillary back with the home key

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

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1. Press the home key.

• The capillary is moved back into the work area.

•The home function is terminated.

6.13.5 Setting the offset.

For capillaries of different lengths, an offset can be set. This defines a limit for the home function.

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1. Change the offset using the arrow keys.

• The value for the offset is displayed in the status field.

6.13.6 Terminate the home function.

1. Press Back manual.

2. Move the capillary manually using the

The home function is terminated.

joystick.

6.14 Clean function

With this function you can move the capillary over a defined distance out of the medium. In the process, outside contamination is removed from the capillary at the medium boundary.

6.14.1 Clean menu and parameters

Abb. 6-5:Menu – Clean

Fig. 6-5: Menu – Clean

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Parameter Value Range of values Increment Standard

Home speed Set the speed of the clean

5 – 10000 5 7500

function

Clean distance Set distance in μm 0 – 20000 5 10000

6.14.2 Setting the parameter for Clean

Set the Clean distance above the medium boundary

1. Set Home speed.

2. Set Clean distance.

3. Close the menu.

6.14.3 Execute the Clean function

Prerequisites

• Parameters are set for Clean.

• Clean is assigned to a softkey.

1. Press Clean. The screen for Clean appears.

2. Execute the function with Clean.

• The capillary is moved out of the medium with the set parameters.

6.14.4 Terminate the Clean function.

1. Press Back manual.

• The function is terminated.

• The application screen appears.

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6.15 PiezoXpert function

6.15.1 PiezoXpert menu and parameters

Abb. 6-6:Menu – PiezoXpert

Fig. 6-6: Menu – PiezoXpert

Parameter Value Range of values Increment Standard

Synchr. PiezoXp.

Switch synchronization between InjectMan 4 and

OFF/ON

PiezoXpert on or off

PiezoXp. speed Set the advance rate of the

5 – 10000 5 300 manipulator in μm/s while the PiezoXpert triggers a pulse.

PiezoXp. dist. Set the length of the

1 – 2000 1 20 movement step of the manipulator in μm while the PiezoXpert triggers a pulse.

PiezoXp. axial Axial movement on or off

ON/OFF – OFF (corresponds to the set injection angle) On = movement in the X and Y-axis. Off = movement in the X-axis

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6.15.2 Execute the PiezoXpert function

Prerequisites

• A PiezoXpert is connected.

1. Activate Synchr. PiezoXp.

2. Set PiezoXp. speed.

3. Set PiezoXp. dist.

4. Select PiezoXp. axial.

5. Close the menu.

6. Trigger the function with Channel A on the PiezoXpert.

• The function is executed.

6.16 Installation function

This function provides settings for finely adjusting the motor module and the control board. Parameters in the Installation menu disable identical softkey functions.

6.16.1 Installation menu and parameters

Abb. 6-7:Menu – Installation

Fig. 6-7: Menu – Installation

6.16.2 Installation parameters

Parameter Value Range of values Increment Standard

Side Set the mounting side LEFT/RIGHT – RIGHT

Angle Set the capillary angle in

0° – 90° 1 – degrees

6.16.3 Control board parameters

Parameter Value Range of values Increment Standard

LCD contrast Set the display contrast 65 % – 75 % 1 70 %

LCD light Set the brightness level of the

0 % – 100 % 1 100 % display

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Parameter Value Range of values Increment Standard

Beeper Set the volume 0 – 100 % 1 50 %

Display

Set the coordinate display OFF/ON – On

coordin.

6.16.4 Module parameters

Parameter Value Range of values Increment Standard

Invert X-axis Reverse the direction of

OFF/ON – OFF movement of the motor

Invert Y-axis Reverse the direction of

OFF/ON – OFF movement of the motor

Invert Z-axis Reverse the direction of

OFF/ON – OFF movement of the motor

X-axis off Switch the motor of the

OFF/ON – OFF movement axis on or off

Y-axis off Switch the motor of the

OFF/ON – OFF movement axis on or off

Z-axis off Switch the motor of the

OFF/ON – OFF movement axis on or off

Upper limit Define the upper limit EXECUTE – –

6.17 Function function

Function allows you to reset the parameters and coordinates and to move the modules to

the middle position.

6.17.1 Function menu and parameters

Abb. 6-8:Menu – Function

Fig. 6-8: Menu – Function

Parameter Value Range of values

Zero coordin. Reset all coordinates to zero Execute

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Parameter Value Range of values

Center motors Move step motors of the motor module to the middle

position

User default Reset the settings to the delivery status Execute

Execute

6.17.2 Executing Zero coordin.

1. Select the parameters and confirm with Enter.

2. Confirm Execute with Enter.

• All coordinates are reset to zero.

• Saved positions are deleted.

• Set limits are deleted.

6.17.3 Executing Center motors

Prerequisites

• No capillary holder is clamped.

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Select the parameters and confirm with Enter.

2. Confirm Execute with Enter.

• X-motor and Y-motor are moved to the middle position.

• Z-motor is moved to a 20/80 position.

• All coordinates are reset to zero.

• Saved positions are deleted.

• Set limits are deleted.

6.17.4 Executing User default

1. Select the parameters and confirm with Enter.

2. Confirm Execute with Enter.

• All parameters are reset to the factory settings.

• The application screen appears.

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6.18 Softkeys function

With this function you can assign programs to free softkeys. Assigned softkeys are indicated with a lock symbol.

6.18.1 Softkeys menu and parameters

Abb. 6-9:Menu – Softkeys

Fig. 6-9: Menu – Softkeys

Parameter Value Range of values

Softkey 1 Set the function

Softkey 2 Set the function

Softkey 3 Set the function

Softkey 4 Set the function

Softkey 5 Set the function

Joystick key Set the function for the joystick key No function

Switch position Switch fine Joystick off Inject

Parameter Function

No function Softkey has no function

Pos 1 Save, overwrite or delete a position

Pos 2 Save, overwrite or delete a position

Pos 3 Save, overwrite or delete a position

Pos 4 Save, overwrite or delete a position

Pos 5 Save, overwrite or delete a position

Y-axis off Switch the control of the movement axis on or off

Axial Change vertical movement to axial movement

Limit Activate or deactivate the vertical or horizontal (X-axis) limit

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Parameter Function

Step injection Activate the Step injection function

Limit up Change the value for Z-axis Limit upwards

Limit down Change the value for Z-axis Limit downwards

Clean Axial movement out of the working range

Z-axis only Only control of movements in the Z-axis. The control of movements in

the X and Y-axis is disabled

LCD light Set the brightness level of the display

Beeper Set the volume

Joystick off Deactivates all joystick movements

X-axis off Switch the control of the movement axis on or off

Z-axis off Switch the control of the movement axis on or off

X-axis only Only control of movements in the X-axis. The control of movements in

the Y and Z-axis is disabled

Y-axis only Only control of movements in the Y-axis. The control of movements in

the X and Z-axis is disabled

Parameter Range of values Function

Joystick key No function Deactivate the function of the joystick key

Switch position Click once to go to the next saved position

Switch fine Click once to go to the fine or x-fine working range

Joystick off Click once to switch the joystick on or off

Inject Click once to activate the function

6.18.2 Executing Softkeys

1. Select the free softkey and confirm with Enter.

2. Select the desired parameter and confirm with Enter.

3. Close the menu.

• The selected parameter is assigned to the free softkey.

• The parameter appears on the application screen.

Operation

InjectMan

English (EN)

6.18.3 Executing Joystick key

Prerequisites The My application application has been selected.

1. Select Joystick key and confirm with Enter.

2. Select the desired function and confirm with Enter.

3. Close the menu.

• The function is assigned to the joystick key.

• The selected function appears on the application screen.

6.19 Change appl. function

With this function you can define each application as the start screen or reactivate the default screen.

6.19.1 Change appl. menu and parameters

Abb. 6-10:Menu – Change appl.

Fig. 6-10: Menu – Change appl.

Parameter Value Range of values

Start display Reactivate start screen to select an application Execute

Application Define the selected application as the start screen Adherent cell inj

ICSI Dev. biology Physiology My application

6.19.2 Defining the selected application as the start screen

1. Select Start display.

2. Confirm with Execute.

3. Close the menu.

• The micromanipulator starts with the selected application.

Operation

InjectMan

English (EN)

6.19.3 Defining the application as the start screen

1. Select the application.

2. Confirm with Enter.

3. Close the menu.

• The micromanipulator always starts with the defined application.

6.20 Service function

6.20.1 Service menu and parameters

Abb. 6-11:Service

Fig. 6-11: Service

Parameter Value Range of values

Selftest Move the motor module in all three axes and output

Execute

error message

Device info Display software version Execute

Set clock Set date and time YYYY-MM-DD

00:00

Movements Show movement routes of the modules (X, Y, Z) Execute

Show errors Display last 10 error messages Execute

Demo run Save the position and move to it Execute

Operation

InjectMan

English (EN)

6.20.2 Execute the Selftest function

CAUTION! Risk of crushing between the modules

The modules automatically move in all spatial axes.

 Do not reach into the movement range of the modules.

1. Select Selftest and confirm with Enter.

2. Confirm Execute with Enter. The screen for the Selftest function appears.

3. Execute Selftest with Start. The motors move up to the limit stops.

4. Terminate Selftest with Stop.

6.21 Resetting parameters to the factory settings

6.21.1 Perform reset

Reset all parameters to the factory settings.

1. Keep the home key pressed.

2. Switch on the device at the mains power switch. The screen for GENERAL RESET appears.

3. Execute the function with Yes.

• All parameters are now reset to the factory settings.

6.21.2 Carrying out a reset in the menu

Prerequisites

• An application has been selected.

1. Press the menu key.

2. Select the Function menu.

3. Press Enter.

4. Select User default and confirm with Execute.

• All parameters are now reset to the factory settings.

6.22 Remote computer control of the InjectMan 4

You can control the InjectMan 4 by means of a terminal program remotely via computer. Computer control by means of a terminal program is only recommended for certain applications and is not described in this operating manual. A special operating manual for this functionality is available at the following Internet address: www.eppendorf.com

Eppendorf does not provide any support for controlling the InjectMan 4 by means of a terminal program.

7 Troubleshooting

7.1 General errors

7.1.1 Motor module

Symptom/message Cause Remedy

Motor axes move in the wrong direction or do not match joystick movement.

• Motor module mounted incorrectly.

• Incorrect installation parameters entered.

• Direction of movement of axis inverted.

 Compare the installation

parameters with the structure of the motor module.

 Check the orientation and

structure of the modules.

 Check the connections of

the modules at the control board.

 Cancel the inversion of the

axis.

7.1.2 Capillary

Symptom/message Cause Remedy

Capillary moves too fast or too slow.

Capillary only moves to the side or vertically.

Capillary does not move down far enough.

• Radius of working range incorrectly set.

• Incorrect acceleration factor.

• Y-axis is disabled.

•The Z-axis Limit function is active.

• Capillary is incorrectly adjusted.

 Set the radius using the

selection dial or in the Speed menu.

 In the Installation menu, set

the value for the Dyn-factor parameter.

 The Y off function disabled.

 Deactivate the Z-axis Limit

function.

 Readjust the capillary.

Troubleshooting

InjectMan

English (EN)

7.1.3 Control board and display

Symptom/message Cause Remedy

Device does not respond to keystroke when Home function is active.

• Function is active.

 Press the home key again.

The capillary moves down.

 Press the Back manual

softkey.

 Move the joystick.

Troubleshooting

InjectMan

English (EN)

Symptom/message Cause Remedy

The display does not show anything or the device cannot be activated although the device is connected.

• The power cable or power plug is loose.

• The device is switched off.

7.1.4 Joystick

Symptom/message Cause Remedy

Outer movement range of the joystick does not work.

• Outer movement range is deactivated.

7.1.5 Software and parameters

Symptom/message Cause Remedy

Parameters are not accessible for certain operations.

–

 Check the power cable or

the power plug.

 Switch the device on.

 In the Installation menu, set

the value for Dyn-mode to

ON.

 Enter parameters again.  Carry out a reset and reset

all parameters to the works settings.

 Readjust device.

7.2 Error messages

7.2.1 Warnings

Symptom/message Cause Remedy

WARNING 1 • FemtoJet is not connected.

 Disconnect the data cable to

the FemtoJet and connect it again.

 Switch on the FemtoJet.

WARNING 3 • X-module is not connected.

 Switch off the device.  Connect the plug of the

X-module to the control board and tighten.

 Switch the device on.

WARNING 4 • Y-module is not connected.

 Switch off the device.  Connect the plug of the

Y-module to the control board and tighten.

 Switch the device on.

Troubleshooting

InjectMan

English (EN)

Symptom/message Cause Remedy

WARNING 5 • Z-module is not connected.  Switch off the device.

 Connect the plug of the

Z-module to the control board and tighten.

 Switch the device on.

WARNING 6 • Synchronization error with

FemtoJet during an injection.

7.2.2 Error

Symptom/message Cause Remedy

ERROR 10 – ERROR 99 • Technical error.

 Set another synchronization

for Synchr. inject..

 Set the synchronization

IMMEDIATE, LIMIT or PRESSURE.

 Switch the device off and

back on.

 Reset the parameters to the

factory settings.

 Contact Eppendorf Service.

Maintenance

InjectMan

English (EN)

8 Maintenance

8.1 Replacing fuses

DANGER! Electric shock.

 Switch off the device and disconnect the power plug before starting

maintenance or cleaning work.

The fuse holder is located between the mains connection socket and the mains power switch. The fuses may only be replaced with the same type of fuse.

1. Disconnect the mains plug.

2. Pull the fuse holder out completely.

3. Replace the defective fuse.

4. Insert the fuse holder.

8.2 Cleaning

DANGER! Electric shock as a result of penetration of liquid.

 Switch off the device and disconnect the power plug before starting cleaning

or disinfection work.

 Do not allow any liquids to penetrate the inside of the housing.  Do not spray clean/spray disinfect the housing.  Only plug the device back in if it is completely dry, both inside and outside.

NOTICE! Damage from the use of aggressive chemicals.

 Do not use any aggressive chemicals on the device or its accessories, such as

strong and weak bases, strong acids, acetone, formaldehyde, halogenated hydrocarbons or phenol.

 If the device has been contaminated by aggressive chemicals, immediately

clean it by means of a mild cleaning agent.

Prerequisites

• Clean at least every 4 weeks.

1. Wipe the painted parts and the aluminum surfaces with a cloth and mild detergent.

2. Wipe with a dry cloth afterwards.

Maintenance

InjectMan

English (EN)

8.3 Service and maintenance

Application-specific service and safety inspections are not required

The Eppendorf AG service team is available to service and certify your device.

Service provisions:

•Service

• Operational qualification (OQ) according to manufacturer's specifications

• Electrical safety testing according to country-specific regulations

•Software update

Information on the services offered can be found on our webpage: www.eppendorf.com/ epservices.

8.4 Disinfection/decontamination

Choose the disinfection method which corresponds to the legal regulations and guidelines in place for your range of application. If you have any questions on cleaning, disinfection, decontamination, or the cleaning agents to be used, please contact Eppendorf AG Application Support. The relevant contact details are provided on the back of this operating manual.

Prerequisites

• All device parts are cleaned.

• A disinfectant containing alcohol (such as isopropanol or spirit) is available

1. Wipe all parts of the device with a cloth and the disinfectant.

Technical data

InjectMan

English (EN)

9 Technical data

Motor module

Travel, maximum 20 mm

Step motors X-module, Y-module, Z-module

Weight 2150 g

Module (X,Y,Z)

Type Step motors

Step size

< 20 nm

(calculated resolution)

Speed, maximum 10000 μm/s

Width 129 mm

Depth 51 mm

Height 36 mm

Weight 570 g

Swivel joint

Direction of rotation -45° – +90°

Capillary change Direction of rotation forwards

Sample change Direction of rotation backwards

Angle head

Operating angle 0° – 90°

Control board

Control Joystick

Working range coarse, fine, x-fine

Width 205 mm

Depth 288 mm

Height 152 mm

Weight 1800 g

9.1 Power supply

Control board

Mains connection 120 – 240 V AC, 50 – 60 Hz

Power consumption 30 W

Protection class I

Overvoltage category II (IEC 61010-1)

Micro fuse T 1.6 A, 250 V

9.2 Interfaces

Module (X, Y, Z) SubD25, female

PC/external device Serial interface

SubD9, male

Service connection USB

9.3 Ambient conditions

Environment For indoor use only.

Ambient temperature 15 °C – 35 °C

Max. relative humidity 30 % – 65 %

Degree of pollution 2 (IEC 664)

Technical data

InjectMan

English (EN)

Transport, storage and disposal

InjectMan

English (EN)

10 Transport, storage and disposal

10.1 Storage

Air temperature Relative humidity Atmospheric

pressure

In transport packaging

Without transport packaging

-25 °C – 55 °C 10 % – 95 % 70 kPa – 106 kPa

-5 °C – 45 °C 10 % – 95 % 70 kPa – 106 kPa

10.2 Decontamination before shipment

If you are shipping the device to the authorized Technical Service for repairs or to your authorized dealer for disposal please note the following:

WARNING! Risk to health from contaminated device

1. Please observe the information in the decontamination certificate, which is available as a PDF file on our webpage (www.eppendorf.com/ decontamination).

2. Decontaminate all the parts you would like to dispatch.

3. Include the fully completed decontamination certificate in the package.

10.3 Transport

NOTICE! Damage to the control board as a result of incorrect handling.

 Grasp the control board on the housing.  Do not lift the control board using the joystick.  Never place the control board on the joystick.

Air temperature Rel. humidity Atmospheric pressure

General transport -25 °C – 60 °C 10 % – 95 % 30 kPa – 106 kPa

Air freight -40 °C – 55 °C 10 % – 95 % 30 – 106 kPa

Carry out the following steps before transport:

1. Dismantle the module unit before transport.

2. Attach the supplied transport securing devices according to the red supplement sheet.

3. Only use original packaging for transport.

Transport, storage and disposal

InjectMan

English (EN)

10.4 Disposal

In case the product is to be disposed of, the relevant legal regulations are to be observed.

Information on the disposal of electrical and electronic devices in the European Community:

Within the European Community, the disposal of electrical devices is regulated by national regulations based on EU Directive 2002/96/EC pertaining to waste electrical and electronic equipment (WEEE).

According to these regulations, any devices supplied after August 13, 2005, in the business-to-business sphere, to which this product is assigned, may no longer be disposed of in municipal or domestic waste. To document this, they have been marked with the following identification:

Because disposal regulations may differ from one country to another within the EU, please contact your supplier if necessary.

In Germany, this is mandatory from March 23, 2006. From this date, the manufacturer has to offer a suitable method of return for all devices supplied after August 13, 2005. For all devices supplied before August 13, 2005, the last user is responsible for the correct disposal.

Ordering Information

InjectMan

English (EN)

11 Ordering Information

11.1 InjectMan 4

Order no. (International)

Order no. (North America)

Description

InjectMan 4

5192 000.019 5192000019 Mains/Power plug Europe 5192 000.027 5192000027 Mains/Power plug USA/Japan 5192 000.035 5192000035 Mains/Power plug UK/Hong Kong 5192 000.043 5192000043 Mains/Power plug Australia 5192 000.051 5192000051 Mains/Power plug China 5192 000.060 5192000060 Mains/Power plug Argentina

11.2 Accessories for InjectMan 4

Order no. (International)

5181 070.015

Order no. (North America)

Description

Data cable

Connect FemtoJet/FemtoJet express to

920005845

micromanipulator

5181 150.051 920005799 Foot control

Data cable 5181

5181 150.094 920005837 Connect PC or PiezoXpert to micromanipulator

Antivibration Pad

5181 301.009 920007945 XS 5181 303.001 920007953 S 5181 305.004 920007961 M 5181 307.007 920007970 L 5181 309.000 920007988 XL

Spare parts kit

5192 071.005 5192071005 1 slider (complete), 2 machine screws M2.5×6

(swivel joint), 2 set screws (angle head), 2 compression springs (knurled screw angle head)

Positioning aid

2 pieces

5192 072.001 5192072001 for universal capillary holder

Headstage holder

5192 073.008 5192073008 for preamplifier

11.3 Tools for InjectMan 4

Ordering Information

InjectMan

English (EN)

Order no. (International)

Order no. (North America)

Description

Allen torque screwdriver

5192 074.004 5192074004

3 mm

Allen screwdriver

5192 075.000 5192075000

1.3 mm

Allen key

7 pieces

5192 076.007 5192076007 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6mm 5192 077.003 5192077003 Toolb ag

11.4 Microscope adapter

Order no. (International)

Order no. (North America)

Description

Adapter for microscope

Leica 1

5192 301.000 5192301000 DMI3000 B, 3000 M, 4000 B, 5000 B, 5000 M,

6000 B, DM IRB E, HC, DM IRE 2

Adapter for microscope

Leica 2

5192 302.007 5192302007 DM IL LED, HC

Adapter for microscope

Olympus 1

5192 306.002 5192306002 IX50, IX51, IX70, IX80, IX81

Adapter for microscope

Olympus 2

5192 307.009 5192307009 IX53, IX73

Adapter for microscope

Nikon 1

5192 316.008 5192316008 Eclipse® Diaphot 200, 300, Eclipse® Ti-E, Ti-U,

Ti-S, TE200, TE300, TE2000

Adapter for microscope

Zeiss 1

5192 311.006 5192311006 Axiovert® 100, AxioObserver A1, D1, Z1

Adapter for microscope

Zeiss 2

5192 312.002 5192312002 Axio Vert.A1

Ordering Information

InjectMan

English (EN)

11.5 Accessories for adapters for microscope

Order no. (International)

Order no. (North America)

Description

Universal stand

for micromanipulators

5192 325.007 5192325007 TransferMan 4m/4r, InjectMan 4

Adapter bridge

for micromanipulators

5192 321.001 5192321001 TransferMan 4m/4r, InjectMan 4

11.6 Capillaries for research applications

Order no. (International)

Order no. (North America)

Description

epT.I.P.S. Racks, Microloader

Eppendorf Quality, 2 racks of 96 tips

5242 956.003 930001007 0.5 - 20 μL, light gray, length: 100 mm

Femtotips

5242 952.008 930000035 20 pieces

Femtotips II

5242 957.000 930000043 20 pieces

TransferTip (ES)

5175 107.004 930001040 25 pieces, sterile

Piezo Drill Tip

for mouse ICSI

5175 220.005 930001091 25° angle, 6 mm rigid flange, sterile, set of 25

Piezo Drill Tip (ES)

for transfer of ES cells into blastocysts

5175 250.001 930001104 25° angle, 6 mm rigid flange, 15 μm inner

diameter, sterile, set of 25

Ordering Information

InjectMan

English (EN)

11.7 Capillaries

Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC).

Order no. (International)

Order no. (North America)

Description

VacuTip

5175 108.000 930001015 25 pieces, sterile

VacuTips FCH

5175 240.006 5175240006 25 pieces

TransferTip-RP (ICSI)

5175 114.000 930001074 25 pieces, sterile

TransferTip-F (ICSI)

5175 106.008 930001031 25 pieces, sterile

TransferTip-R (ICSI)

5175 113.004 930001066 25 pieces, sterile

Polar Body Biopsy Tip MML

5175 210.000 5175210000 25 pieces

Polar Body Biopsy Tip FCH

5175 230.000 5175230000 25 pieces

IMSI/TESE Tip

5175 117.000 5175117000 25 pieces, sterile

11.8 Universal capillary holder and accessories

Order no. (International)

Order no. (North America)

Description

Universal capillary holder

For holding Femtotips, Femtotips II or grip heads

5176 190.002 920007392 Including grip head 0, adapter for Femtotips and

spare O-rings

Grip head 0

5176 210.003 920007414 For microcapillaries with an outer diameter of

1.0 mm to 1.1 mm, set of 2

Grip head 1

5176 212.006 920007708 For microcapillaries with an outer diameter of

1.2 mm to 1.3 mm, set of 2

Grip head 2

5176 214.009 920007716 For microcapillaries with an outer diameter of

1.4 mm to 1.5 mm, set of 2

Ordering Information

InjectMan

English (EN)

Order no. (International)

Order no. (North America)

Description

Grip head 3

5176 207.002 920007406 For microcapillaries with an outer diameter of

0.7 mm to 0.9 mm, set of 2

O-ring set

5176 196.000 920005870 For grip heads 0-3 with removal tool

Service kit

For grip heads and capillary holder

5176 195.004 920005888 Consisting of O-ring set, adapter for Femtotips

11.9 CellTram and accessories

Products are registered as a medical device in Europe (according to the Medical Device Directive (MDD) 93/42/EEC).

Order no. (International)

Order no. (North America)

Description

5176 000.017 920002021 CellTram Air 5176 000.025 920002030 CellTram Oil 5176 000.033 920002111 CellTram vario

Pressure tube

5176 114.004 920002081 1 m

11.10 FemtoJet

Order no. (International)

Order no. (North America)

Description

FemtoJet

programmable micro-injector with integrated

pressure supply 5247 000.013 5247000013 Mains/Power plug Europe 5247 000.021 920010504 Mains/Power plug Canada 5247 000.030 5247000030 Mains/Power plug UK / Hong Kong 5247 000.048 5247000048 Mains/Power plug Japan 5247 000.056 5247000056 Mains/Power plug South America 5247 000.072 5247000072 Mains/Power plug Australia 5247 000.080 5247000080 Mains/Power plug China

11.11 FemtoJet express

Ordering Information

InjectMan

English (EN)

Order no. (International)

Order no. (North America)

Description

FemtoJet express

programmable micro-injector 5248 000.017 5248000017 Mains/Power plug Europe 5248 000.033 5248000033 Mains/Power plug UK / Hongkong 5248 000.041 5248000041 Mains/Power plug Japan 5248 000.050 920010521 Mains/Power plug USA and Canada 5248 000.076 5248000076 Mains/Power plug Australia 5248 000.084 5248000084 Mains/Power plug China

11.12 Accessories for FemtoJet/FemtoJet express

Order no. (International)

Order no. (North America)

Description

Hand control

5252 076.010 5252076010 for remote-controlling the FemtoJet / FemtoJet

express 5181 150.051 920005799 Foot control

Data cable

Connect FemtoJet/FemtoJet express to

micromanipulator 5181 070.015 920005845

Pressure tube

for connecting the FemtoJet express to an external

pressure supply 5248 200.008 – Length 2.5 m, incl. 2 couplings G 1/4 inch and 1/

4 inch 18 NPT

Injection tube

5246 164.004 920007431 2 m, for universal capillary holder

Ordering Information

InjectMan

English (EN)

11.13 PiezoXpert

Order no. (International)

Order no. (North America)

Description

Eppendorf PiezoXpert

for piezo-assisted micromanipulation 5194 000.016 – with mains/power plug EU 5194 000.024 5194000024 with mains/power plug USA/Japan 5194 000.032 5194000032 with mains/power plug UK/Hong Kong 5194 000.059 5194000059 with mains/power plug Australia 5194 000.067 5194000067 with mains/power plug China 5194 000.075 5194000075 with mains/power plug Argentina

Index

InjectMan

English (EN)

Index

Ambient conditions ................................ 85

Cable....................................................... 38

Capillary

Replace ............................................. 54

Cleaning ................................................. 82

Computer control

Terminal program............................. 78

Connection

External device ................................. 43

Decontamination .................................... 86

Deleting

positions ........................................... 58

Disinfectant ............................................ 83

Display

Coordinates ...................................... 46

Menu ................................................ 47

Parameter ......................................... 47

Disposal .................................................. 87

First set-up ............................................. 39

Function

Change appl...................................... 76

Clean................................................. 68

Function ............................................ 72

Home ................................................ 66

Inject ................................................. 63

Installation ........................................ 71

Service............................................... 77

Softkeys............................................. 74

Speed ................................................ 62

Step injection..................................... 65

Initialization ............................................53

Installation

First set-up ........................................39

Installation parameters .....................39

Selecting the location........................20

Limit

Horizontal limit .................................61

Lower limit ........................................ 59

Upper limit ........................................ 60

Vertical limit......................................59

X-limit ...............................................61

Z limit................................................59

Main menu ..............................................51

Moving to

positions............................................58

Overwriting

positions............................................59

Save...................................................57

Parameter input ...................................... 52

Power supply

Control board ....................................85

Reset .......................................................78

Sample

Change ..............................................56

Selecting the location..............................20

Service

Safety inspections .............................83

Index

InjectMan

English (EN)

Service provisions ............................ 83

Software navigation................................ 52

Speed range ........................................... 17

coarse................................................ 17

fine .................................................... 17

x-fine................................................. 17

Standby................................................... 53

Storage ................................................... 86

Switching off .......................................... 53

Switching on........................................... 53

Unpacking .............................................. 20

X-limit..................................................... 61

Z limit ..................................................... 59

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Eppendorf AG · 22331 Hamburg · Germany eppendorf@eppendorf.com · www.eppendorf.com

Eppendorf InjectMan 4 User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

Table of contents

1 Operating instructions

1.1 Using this manual

1.2 Danger symbols and danger levels

1.2.1 Danger symbols

1.2.2 Danger levels

1.3 Symbols used

2 Product description

2.1 Delivery package

2.1.1 Tools

2.1.2 Accessories

2.2 Features

2.3 Main illustration

2.3.1 Motor module

2.3.2 Control board

2.4 Control panel

2.5.2 Direction of movement of the joystick

2.5.3 Joystick key functions

2.6 Speed ranges

3Safety

3.1 Intended use

3.2 Warnings for intended use

3.3 Warning signs on the device

3.4 Information on product liability

4 Installation

4.1 Preparing installation

4.1.1 If there is any damage, make a claim

4.1.2 Delivery incomplete

4.1.3 Microscope adapter assembly

4.2 Selecting the location

4.4.1 Mounting the Z-module

4.4.2 Mounting the Y-module

4.4.3 Mounting the X-module

4.4.4 Mounting the angle head

4.5 Inserting the capillary holder into the angle head

4.6 Inserting the capillary into the capillary holder

4.6.1 Inserting Femtotips

4.7 Setting the injection angle

4.8 Aligning the motor module

4.8.1 Aligning the height

4.8.2 Aligning the depth

4.8.3 Aligning the width

4.8.4 Aligning the angle head

4.9 Entering mounting parameters

4.9.1 Microscope and adapter

4.10 Converting the swivel joint for left side mounting

4.11 Converting the angle head for left side mounting

4.12 Connect the motor module to the control board

4.13 Setting installation parameters

4.13.1 First set-up wizard

4.14 Connecting an external device

4.14.1 Connecting the FemtoJet

4.14.2 Connecting the PiezoXpert

4.14.3 Connecting the Computer

4.14.4 Connecting two devices

5.2.1 Application parameter

5.2.2 Application – Adherent cell injection

5.2.3 Application – ICSI

5.2.4 Application – Developmental biology

5.2.5 Application – Physiology

5.2.6 Application – My application

5.3 Main menu

5.4 Navigating the menu

5.4.1 Entering or changing parameters

6Operation

6.1 Switching the device on or off

6.1.1 Switching on the device

6.1.2 Switching the device off

6.2 Activating or deactivating the control board

6.2.1 Activating the control board

6.2.2 Deactivating the control board

6.3 Defining the start screen

6.3.1 Defining the application

6.3.2 Defining the selected application

6.4 Replacing the capillary

6.4.1 Manually positioning the capillary