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InjectMan
Operating manual
®
4
Copyright© 2013 Eppendorf AG, Hamburg.
Eppendorf
®
and the Eppendorf logo are registered trademarks of Eppendorf AG,
Hamburg, Germany.
InjectMan
®
4, FemtoJet® and Eppendorf PiezoXpert® are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Registered trademarks are not marked in all cases with ™ or ® in this manual.
No part of this publication may be reproduced without the prior permission of the
copyright owner.
5192 900.010-00/072013
Table of contents
InjectMan
®
English (EN)
Operating manualInjectMan® 4seeon p.Fig.Tab.p.
English (EN)Operating manual
Table of contents
1 Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Delivery package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.1 Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1.2 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.3 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.3.2 Control board. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4 Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.5.1 Dynamic range. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.5.2 Direction of movement of the joystick. . . . . . . . . . . . . . . . . . . . . . 16
2.5.3 Joystick key functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.6 Speed ranges. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4
3
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.3 Warning signs on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1.1 If there is any damage, make a claim . . . . . . . . . . . . . . . . . . . . . . 20
4.1.2 Delivery incomplete . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.1.3 Microscope adapter assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
4.3 Mounting overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.3.1 Module (X, Y, Z). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.3.2 Z-module holder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.3.3 Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.3.4 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4.3.5 Swivel joint . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
Table o f co nte nts
InjectMan
4
®
4
English (EN)
4.4 Mounting the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.4.1 Mounting the Z-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
4.4.2 Mounting the Y-module. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
4.4.3 Mounting the X-module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
4.4.4 Mounting the angle head . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4.5 Inserting the capillary holder into the angle head . . . . . . . . . . . . . . . . . . . . 28
4.6 Inserting the capillary into the capillary holder . . . . . . . . . . . . . . . . . . . . . . 29
4.6.1 Inserting Femtotips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
4.7 Setting the injection angle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
4.8 Aligning the motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.8.1 Aligning the height . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.8.2 Aligning the depth . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
4.8.3 Aligning the width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4.8.4 Aligning the angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4.9 Entering mounting parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4.9.1 Microscope and adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
4.9.2 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.9.3 Angle head. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
4.10 Converting the swivel joint for left side mounting . . . . . . . . . . . . . . . . . . . . 34
4.11 Converting the angle head for left side mounting . . . . . . . . . . . . . . . . . . . . 37
4.12 Connect the motor module to the control board. . . . . . . . . . . . . . . . . . . . . . 38
4.13 Setting installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
4.13.1 First set-up wizard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
4.14 Connecting an external device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
4.14.1 Connecting the FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
4.14.2 Connecting the PiezoXpert . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
4.14.3 Connecting the Computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
4.14.4 Connecting two devices. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
5 Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.1 Display. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.1.1 Application display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
5.1.2 Display of coordinates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
5.1.3 Menu display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.2 Applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.2.1 Application parameter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.2.2 Application – Adherent cell injection . . . . . . . . . . . . . . . . . . . . . . 48
5.2.3 Application – ICSI . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
5.2.4 Application – Developmental biology . . . . . . . . . . . . . . . . . . . . . . 49
5.2.5 Application – Physiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.2.6 Application – My application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.3 Main menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
5.4 Navigating the menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
5.4.1 Entering or changing parameters . . . . . . . . . . . . . . . . . . . . . . . . . 52
Table of contents
InjectMan
®
English (EN)
6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1 Switching the device on or off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1.1 Switching on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.1.2 Switching the device off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.2 Activating or deactivating the control board. . . . . . . . . . . . . . . . . . . . . . . . . 53
6.2.1 Activating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.2.2 Deactivating the control board . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
6.3 Defining the start screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.3.1 Defining the application . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.3.2 Defining the selected application . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.4 Replacing the capillary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
6.4.1 Manually positioning the capillary . . . . . . . . . . . . . . . . . . . . . . . . 55
6.4.2 Automatically positioning the capillary . . . . . . . . . . . . . . . . . . . . . 55
6.5 Changing the sample . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.6 Changing the speed range . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
6.6.1 Changing parameters using the selection dial . . . . . . . . . . . . . . . 56
6.6.2 Changing parameters in the menu . . . . . . . . . . . . . . . . . . . . . . . . 57
6.7 Capillary positions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.7.1 Saving a position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
6.7.2 Moving to the position using the softkey . . . . . . . . . . . . . . . . . . . 58
6.7.3 Moving to the position using the joystick key . . . . . . . . . . . . . . . . 58
6.7.4 Deleting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
6.7.5 Overwriting a stored position . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.8 Vertical limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.8.1 Defining the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
6.8.2 Deleting the lower limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.8.3 Defining the upper limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.8.4 Deleting the upper limit. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
6.9 Horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.9.1 Defining the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.9.2 Deleting the horizontal limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
6.10 Speed function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.10.1 Speed menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.10.2 Setting the parameters for Speed . . . . . . . . . . . . . . . . . . . . . . . . . 62
6.11 Inject function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.11.1 Inject menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
6.11.2 Executing the Inject function . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
6.12 Step injection function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
6.12.1 Step injection menu and parameters. . . . . . . . . . . . . . . . . . . . . . . 65
6.12.2 Executing the Step injection function . . . . . . . . . . . . . . . . . . . . . . 66
4
5
Table o f co nte nts
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English (EN)
6.13 Home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.13.1 Home menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.13.2 Setting the parameter for Home . . . . . . . . . . . . . . . . . . . . . . . . . . 66
6.13.3 Move the capillary out with the home key . . . . . . . . . . . . . . . . . . 67
6.13.4 Move the capillary back with the home key . . . . . . . . . . . . . . . . . 67
6.13.5 Setting the offset. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
6.13.6 Terminate the home function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
6.14 Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
6.14.1 Clean menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
6.14.2 Setting the parameter for Clean . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.14.3 Execute the Clean function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.14.4 Terminate the Clean function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
6.15 PiezoXpert function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
6.15.1 PiezoXpert menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 70
6.15.2 Execute the PiezoXpert function . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.16 Installation function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.16.1 Installation menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.16.2 Installation parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.16.3 Control board parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
6.16.4 Module parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.17 Function function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.17.1 Function menu and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . 72
6.17.2 Executing Zero coordin. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
6.17.3 Executing Center motors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
6.17.4 Executing User default . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
6.18 Softkeys function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
6.18.1 Softkeys menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
6.18.2 Executing Softkeys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
6.18.3 Executing Joystick key. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
6.19 Change appl. function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
6.19.1 Change appl. menu and parameters . . . . . . . . . . . . . . . . . . . . . . . 76
6.19.2 Defining the selected application as the start screen . . . . . . . . . . 76
6.19.3 Defining the application as the start screen . . . . . . . . . . . . . . . . . 77
6.20 Service function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
6.20.1 Service menu and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
6.20.2 Execute the Selftest function. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
6.21 Resetting parameters to the factory settings . . . . . . . . . . . . . . . . . . . . . . . . 78
6.21.1 Perform reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
6.21.2 Carrying out a reset in the menu . . . . . . . . . . . . . . . . . . . . . . . . . . 78
6.22 Remote computer control of the InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . 78
Table of contents
InjectMan
®
English (EN)
7 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.1.1 Motor module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.1.2 Capillary. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.1.3 Control board and display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.1.4 Joystick . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7.1.5 Software and parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7.2.1 Warnings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
7.2.2 Error. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
8 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
8.1 Replacing fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
8.2 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
8.3 Service and maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
8.4 Disinfection/decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
9 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
9.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
9.2 Interfaces. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
9.3 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
10 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
10.1 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
10.2 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
10.3 Transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
10.4 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
4
7
11 Ordering Information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
11.1 InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
11.2 Accessories for InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
11.3 Tools for InjectMan 4 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
11.4 Microscope adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
11.5 Accessories for adapters for microscope . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
11.6 Capillaries for research applications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
11.7 Capillaries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
11.8 Universal capillary holder and accessories. . . . . . . . . . . . . . . . . . . . . . . . . . 91
11.9 CellTram and accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
11.10 FemtoJet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
11.11 FemtoJet express. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
11.12 Accessories for FemtoJet/FemtoJet express . . . . . . . . . . . . . . . . . . . . . . . . . 93
11.13 PiezoXpert. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95
Operating instructions
InjectMan
8
English (EN)
®
4
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time.
Please also note the operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. You will find the current version on
our webpage www.eppendorf.com/worldwide
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and
danger levels:
1.2.1 Danger symbols
Hazard point Cuts
Material damage Electric shock
Biohazard
.
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WAR NIN G Can lead to severe injuries or death.
CAUTION Can lead to light to moderate injuries.
ATTENTION May lead to material damage./Paragraph
1.3 Symbols used
Depiction Meaning
1.
2.
Actions in the specified order
Actions without a specified order
• List:
Text Display or software texts
Additional information
Product description
2 Product description
2.1 Delivery package
Quantity Description
1X-module
1Y-module
1Z-module
1 YZ-connector
1 Swivel joint
1 Angle head
1 Control board
1Mains/power cord
1Cable sheathing
1 Operating manual
2.1.1 Tools
Quantity Description
7 1.5 mm, 2 mm, 2.5 mm, 3 mm, 4 mm, 5 mm, 6 mm Allen key
1 3 mm Allen torque key
1 1.3 mm Allen key
1 Tool bag
InjectMan
English (EN)
®
4
9
2.1.2 Accessories
Quantity Description
1 Connecting cable for FemtoJet/FemtoJet express
2 Positioning aids for capillary holders
1 Spare parts kit
1Label
2.2 Features
The micromanipulator InjectMan 4 has been especially developed for work processes that
require intuitive movement of the capillary.
The InjectMan 4 combines the classical benefits of a mechanical system with the benefits
of an accurate electrically driven system.
Product description
InjectMan
10
®
4
English (EN)
The capillary is controlled by a joystick. A greater forwards or backwards movement of
the joystick results in an acceleration of the capillary movement. The movement ranges
enable moving to any position in the working range of the micromanipulator.
The dynamic movement of the joystick is especially suitable for serial injections and
working techniques that require fast injection movement.
The software control provides predefined applications, freely programmable softkey
functions, a freely programmable application and the storage of different positions all
space coordinates.
2.3 Main illustration
The motor module is mounted on a special microscope adapter or on a free-standing
stand (magnetic). The control board is mechanically separated from the motor module.
Abb. 2-1:InjectMan 4 — right side mounting
1
Fig. 2-1: InjectMan 4 — right side mounting
1 Motor module 2 Control board
2
Product description
InjectMan
®
English (EN)
2.3.1 Motor module
The motor module is made up of three modules. The capillary can be moved in all three
spatial axes due to the layout of the modules. The X-module with the capillary can be
swung out of the working range using the swivel joint. The injection angle of the capillary
can be set to any angle on the angle head.
Abb. 2-2:Motor module
1
4
4
11
2
3
Fig. 2-2: Motor module
1 Z-module
2 YZ-connector
3 Angle head
5
6
4 Y-module
5 Swivel joint
6 X-module
Product description
InjectMan
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®
4
English (EN)
2.3.2 Control board
The control board contains the key pad, the display and the joystick, and, to the side, the
selection dial. The direction of movement and the speed of the joystick are transferred to
the capillary. The responsitivity of the movement and the size of the working range are
predefined in the software setting. On the control panel, the working range can be
selected and modified via the selection dial.
Abb. 2-3:Control board - front
4
Fig. 2-3: Control board - front
1 Joystick
Dynamic movement
2 Display
coarse
2
1
fine
fine
menu
3
home
3 Control panel
4 Selection dial
For increasing or reducing the speed
Abb. 2-4:Control board - rear
Product description
InjectMan
®
English (EN)
4
13
8
Fig. 2-4: Control board - rear
1 Mains power switch On/Off
2 Connection for an external device
Foot control, FemtoJet, FemtoJet
express, PiezoXpert or PC
3 Connection for a Y-module
4 Connection for an X-module
ext. Device
Z-Axis
32
Y-Axis
X-Axis
45
1
SERVICE
7
6
5 Connection for a Z-module
6 Service interface
7 Mains/power connection
8 Fuse
Product description
InjectMan
14
®
4
English (EN)
2.4 Control panel
With the keys on the control panel you can switch on the control board and select the size
of the working range. The softkeys are used to open applications, execute functions,
navigate the menu and set parameters.
Abb. 2-5:Control panel
7
6
5
Fig. 2-5: Control panel
1 home key
Moves the capillary out of the working
range into a defined position
2 Display
Displays the software
3 standby key
Switches the control board on or off or
cancels automatic movements
4 Softkeys 1 – 5
For selecting the application, triggering
the function, navigating or setting
parameter values
fine
x–fine
menu
homecoarse
4
5 menu key
Opens the menu
6 fine/x-fine key
For setting the medium or low speed
range
7 coarse key
For setting the high speed range
1
2
3
Product description
InjectMan
®
English (EN)
2.5 Joystick
The joystick controls the capillary in all three spatial axes. The movement of the capillary
is accelerated the further the joystick is moved forwards or backwards.
Abb. 2-6:Joystick
1
2
Fig. 2-6: Joystick
1 Joystick key 2 Joystick
Controls movements in the X, Y and
Z-axis
2.5.1 Dynamic range
The size of the dynamic range is limited by the movement range of the modules (X, Y and
Z).
When the joystick is moved, the capillary starts moving into the direction of the joystick
movement. The movement of the capillary is accelerated the further the joystick is moved.
The movement stops when the joystick is released.
4
15
Product description
InjectMan
16
®
4
English (EN)
2.5.2 Direction of movement of the joystick
The joystick can be moved along the horizontal plane. This controls the motors of the
X-module and the Y-module. The joystick can be moved in one axis at a time or in a
combination of axes. Rotating the joystick moves the motor module on the vertical axes.
Abb. 2-7:Movement in the X and Y-axis
Move the capillary in the horizontal
direction (X and Y-axis).
Fig. 2-7: Movement in the X and Y-axis
Abb. 2-8:Movement in the Z-axis
Fig. 2-8: Movement in the Z-axis
2.5.3 Joystick key functions
Abb. 2-9:Function
Fig. 2-9: Function
Move the capillary in the vertical
direction (Z-axis).
Trigger function (e.g., trigger injection).
Product description
InjectMan
®
English (EN)
2.6 Speed ranges
There are three speed ranges in the movement range of the joystick. The speed can be set
using the selection dial on the control board and in the Speed menu.
Speed ranges:
• coarse – for a large working range
• fine – for a medium working range
• x-fine – for a small working range
4
17
Safety
InjectMan
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English (EN)
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4
3Safety
3.1 Intended use
The InjectMan 4 has been designed and manufactured for use in biological, chemical and
physical research. It is used for the precise positioning of microcapillaries and similar
tools as well as for the transfer of very small sample volumes.
The InjectMan 4 has been designed and manufactured for research use only.
The InjectMan 4 is intended exclusively for indoor use and for operation by qualified staff.
3.2 Warnings for intended use
WARNING! Risk of injury due to flying capillaries and glass splinters.
If exposed to high pressures, capillaries may detach themselves from the grip
heads and become projectiles.
Capillaries can crack as a result of incorrect handling.
Wear protective goggles.
Never aim capillaries at people.
Use capillaries with an outer diameter that matches the grip head
specifications.
Always mount / dismount capillaries when they are depressurized.
Mount the capillary correctly in the grip head.
Do not touch the capillary with the Petri dish or other objects.
CAUTION! Risk of cuts from broken capillaries.
Capillaries are made of glass and are very fragile.
Wear your personal protective equipment (PPE).
Always mount capillaries depressurized.
Never aim capillaries at people.
Handle the capillaries very carefully.
NOTICE! Mechanical damage to the motor modules.
Excessive load leads to increment errors or destruction of the drive.
Do not drive the modules against mechanical obstructions.
Do not hold any objects near the modules.
Load the motor module with a maximum of 100 g.
WARNING! Damages to health due to infectious liquids and pathogenic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health
Organization, Laboratory Biosafety Manual, as amended) for comprehensive
regulations on the handling of risk group II germs or biological materials.
3.3 Warning signs on the device
Warning symbol Meaning
Warns of the risk of injury caused by capillary tips
Warns of the danger of crushing on the motor module
Safety
InjectMan
English (EN)
®
4
19
Read the operating manual
3.4 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
Installation
InjectMan
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English (EN)
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4
4 Installation
4.1 Preparing installation
NOTICE! Damage to the control board as a result of incorrect handling.
Grasp the control board on the housing.
Do not lift the control board using the joystick.
Never place the control board on the joystick.
Keep the packaging and the transport securing devices for later transport or
storage.
Do not operate the device if there is visible damage to the device itself and/or to
its packaging.
1. Check the packaging for damage.
2. Carefully remove the motor module and the control board from the packaging.
3. Check that everything is included in the delivery.
4. Check the modules, the control board and the accessories for damage.
4.1.1 If there is any damage, make a claim
1. Contact customer service.
4.1.2 Delivery incomplete
1. Contact customer service.
4.1.3 Microscope adapter assembly
The microscope adapter is not included in the scope of delivery and must be ordered
separately.
1. Assemble the microscope adapter in accordance with the assembly instructions for the
microscope adapter.
4.2 Selecting the location
Select the location for the device according to the following criteria:
• Mains connection in accordance with the name plate.
• A bench with a horizontal and even work surface which is designed to support the
weight of the device.
• A mat or table that is cushioned against vibrations.
• The location is protected from direct sunlight and drafts.
The mains/power switch and cutting unit of the mains/power line must be easily
accessible during operation (e.g, residual current circuit breaker).
4.3 Mounting overview
Abb. 4-1:Overview for right-hand side mounting
Installation
InjectMan
English (EN)
®
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21
1
Fig. 4-1: Overview for right-hand side mounting
1 Z-module holder
For left side mounting
2 Microscope adapter
3 Z-module holder
For right side mounting
4 Z-module
5 Y-module
2
3
6 Swivel joint
7 X-module
8 Universal capillary holder
(not included in the delivery package)
9 Angle head
10 YZ-connector
4
5 6
7
8910
Installation
InjectMan
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English (EN)
4.3.1 Module (X, Y, Z)
Abb. 4-2:Y-module
Fig. 4-2: Y-module
1
2
Y
4
3
5
1 Cable
2 Module identification
3 Movable rail
4 Scale
Movement range of the rail
5 Fixed rail
4.3.2 Z-module holder
Abb. 4-3:Z-module holder, front and back
Installation
InjectMan
English (EN)
®
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23
1
4
Fig. 4-3: Z-module holder, front and back
1 Slider
2 Screw
Attach Z-module
4.3.3 Slider
Abb. 4-4:Slider
1
2
3 Screw
Attach Z-module holder to the adapter
4 Stop angle
4
3
2
3
Fig. 4-4: Slider
1 Slider
2 Lock washer
3 Flat washer
4 Screw
Installation
InjectMan
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®
English (EN)
4.3.4 Angle head
Abb. 4-5:Angle head
4
Fig. 4-5: Angle head
1 Identification
For setting the angle
2 Knurled screw
For setting the injection angle
3 Universal capillary holder
(not included in the delivery package)
1
5
4 Positioning aid
5 Knurled screw
2
3
4
For fixing the universal capillary holder
4.3.5 Swivel joint
Abb. 4-6:Swivel joint for right side mounting
Installation
InjectMan
®
4
25
English (EN)
5
1
6
7
2
3
8
4
Fig. 4-6: Swivel joint for right side mounting
1 Slider
Y-module
2 Turntable
3 Allen screws
4 Slider
X-module
5 Stop plate
6 Upper joint
7 Mounting mark
| stands for left side mounting
|| stands for right side mounting
8 Lower joint
4.4 Mounting the motor module
The motor module can be mounted on the right-hand or left-hand side of the microscope
adapter. The following describes mounting on the right-hand side. For mounting on the
left-hand side, the swivel joint and the angle head must be modified.
The motor module is mounted as standard on an inverse microscope. It can also
be mounted on a universal stand. Mounting on a universal stand is described in
the corresponding manual.
Installation
InjectMan
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®
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English (EN)
4.4.1 Mounting the Z-module
Prerequisites
• 3 mm Allen torque key
1. Push the Z-module holder onto the
microscope adapter and tighten.
2. Push the fixed rail onto the Z-module
holder until it reaches the stop angle and
tighten the screw.
The connecting cable faces to the rear.
4.4.2 Mounting the Y-module
Prerequisites
• 3 mm Allen torque key
Installation
InjectMan
®
English (EN)
1. Push the YZ-connector onto the fixed rail
of the Y-module and tighten.
2. Push the Y-module with the
YZ-connector onto the Z-module and
tighten.
4
27
4.4.3 Mounting the X-module
Prerequisites
• 3 mm Allen torque key
1. Push the swivel joint onto the fixed rail
of the X-module and tighten.
Installation
InjectMan
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®
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English (EN)
4.4.4 Mounting the angle head
Prerequisites
• 3 mm Allen torque key
2. Push the swivel joint with the X-module
onto the Y-module and tighten.
1. Push the angle head onto the X-module.
2. Slightly tighten the angle head.
4.5 Inserting the capillary holder into the angle head
Prerequisites
• A universal capillary holder from Eppendorf is available.
• A capillary holder (4 mm diameter) from a different manufacturer is available.
1. Loosen the knurled screw on the angle
head.
2. Insert the capillary holder into the
clamp.
3. Align the capillary holder in such a way
that the capillary tip is located approx.
20 mm above and approx. 20 mm from
the outside of the operating point.
4. Place the positioning aid on the capillary
holder and tighten.
5. Tighten the knurled screw.
4.6 Inserting the capillary into the capillary holder
WARNING! Risk of injury due to flying capillaries and glass splinters.
If exposed to high pressures, capillaries may detach themselves from the grip
heads and become projectiles.
Capillaries can crack as a result of incorrect handling.
Wear protective goggles.
Never aim capillaries at people.
Use capillaries with an outer diameter that matches the grip head
specifications.
Always mount / dismount capillaries when they are depressurized.
Mount the capillary correctly in the grip head.
Do not touch the capillary with the Petri dish or other objects.
Installation
InjectMan
English (EN)
®
4
29
NOTICE! Mechanical damage to the motor modules.
Excessive load leads to increment errors or destruction of the drive.
Do not drive the modules against mechanical obstructions.
Do not hold any objects near the modules.
Load the motor module with a maximum of 100 g.
Standard capillary: Only use the 0 grip head with capillaries with an outer
diameter of 1.0 mm to 1.1 mm. If you would like to use other capillaries, order
the matching grip head.
Installation
InjectMan
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English (EN)
1. Push the capillary into the grip head
until it reaches the stop and tighten the
grip head.
4.6.1 Inserting Femtotips
1. Remove the grip head.
2. Screw the adapter for Femtotips into the universal capillary holder.
3. Screw the Femtotip into the adapter and tighten.
4.7 Setting the injection angle
+
1. Turn the knurled screw to set the
injection angle.
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