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I-26R
User Manual
54 pgs1.41 Mb0

Eppendorf I-26R User Manual

New Brunswick Incubator Shakers
I-26/26R
Operating Manual
Revision E
2
COPYRIGHT:
Copyright © 2012 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior permission of the copyright owner.
The company reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.
TRADEMARKS:
Eppendorf Logo™ are trademarks of Eppendorf AG, Hamburg, Germany.
Excella Inc., USA.
Slo-Blo
Trademarks are not marked in all cases with ™ or ® in this manual.
Eppendorf has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are unintentional.
®
is a registered trademark, and New Brunswick™ and the New Brunswick
®
and Innova® are trademarks owned and registered by New Brunswick Scientific Co.,
®
is a trademark owned and registered by Littlefuse, Inc. Corporation Illinois, USA.
October 4, 2012 Revision E M1324-0050
I-26/26R Incubator Shakers M1324-0050 Operating Manual
3
ALERT! Possible risk to equipment!
This equipment must be operated as described in this manual.
If operational guidelines are not followed, equipment damage
and personal injury can occur.
Please read the entire Operating Manual before attempting to use this equipment.
Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed.
Eppendorf is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by Eppendorf.
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TABLE OF CONTENTS
1 USER INSTRUCTIONS ......................................................................................................................................6
1.1 HAZARD ICONS...............................................................................................................................................6
1.2 DANGER LEVELS.............................................................................................................................................6
1.3 MANUAL CONVENTIONS.................................................................................................................................6
1.4 ABBREVIATIONS.............................................................................................................................................6
2 OVERVIEW..........................................................................................................................................................7
2.1 FRONT VIEW .................................................................................................................................................. 8
2.2 SPECIFICATIONS ............................................................................................................................................. 8
2.3 CERTIFICATIONS............................................................................................................................................. 9
3 INSPECTION, VERIFICATION & UNPACKING OF EQUIPMENT........................................................11
3.1 INSPECTION OF BOXES.................................................................................................................................. 11
3.2 PACKING LIST VERIFICATION....................................................................................................................... 11
3.3 UNPACKING OF EQUIPMENT ......................................................................................................................... 11
4 PREPARING THE LOCATION....................................................................................................................... 12
4.1 PHYSICAL LOCATION....................................................................................................................................12
4.2 ENVIRONMENT .............................................................................................................................................12
4.3 ELECTRICAL REQUIREMENTS ....................................................................................................................... 12
4.4 SPACE REQUIREMENTS.................................................................................................................................13
5 INSTALLING THE I-26/26R............................................................................................................................14
5.1 TOOLS REQUIRED FOR INSTALLATION..........................................................................................................14
5.2 LEVELING A SINGLE SHAKER ....................................................................................................................... 14
5.3 ADDING A BASE ........................................................................................................................................... 15
5.4 PREPARING THE OPTIONAL BASE ................................................................................................................. 15
5.5 MOUNTING THE I-26/26R ON THE OPTIONAL BASE...................................................................................... 17
5.6 INSTALLING THE I-26/26R STACKING KIT....................................................................................................19
5.7 STACKING THE I-26/26R .............................................................................................................................. 20
5.8 STACKING A THIRD I-26/26R.......................................................................................................................21
5.8.1 Load and Speed Graphs ......................................................................................................................... 21
6 FEATURES.........................................................................................................................................................23
6.1 KEYPAD .......................................................................................................................................................23
6.2 LED DISPLAY .............................................................................................................................................. 23
6.3 USER INTERFACE KEYS ................................................................................................................................24
6.4 STATUS INDICATORS .................................................................................................................................... 24
6.5 FUNCTION INDICATORS ................................................................................................................................25
7 GETTING STARTED........................................................................................................................................26
7.1 PLATFORM ASSEMBLIES...............................................................................................................................26
7.2 INSTALLATION OF CLAMPS...........................................................................................................................26
7.3 INSTALLING/SLIDING OUT PLATFORM ..........................................................................................................27
7.4 ELECTRICAL CONNECTIONS ......................................................................................................................... 28
8 OPERATION ...................................................................................................................................................... 30
8.1 STARTING THE SHAKER................................................................................................................................30
8.2 CONTINUOUS (UNLIMITED) RUN ..................................................................................................................31
I-26/26R Incubator Shakers M1324-0050 Operating Manual
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8.3 CHECKING ANY SETPOINT............................................................................................................................ 31
8.4 TIMED FUNCTIONS ....................................................................................................................................... 31
8.5 ALARM FUNCTIONS...................................................................................................................................... 32
8.6 TEMPERATURE SETPOINT .............................................................................................................................33
8.7 TEMPERATURE OFFSET CALIBRATION..........................................................................................................33
8.8 POWER FAILURE........................................................................................................................................... 34
8.9 SPEED CALIBRATION.................................................................................................................................... 34
8.10 TILT SWITCH................................................................................................................................................ 34
9 PREVENTIVE MAINTENANCE.....................................................................................................................36
9.1 CLEANING EXTERNAL SURFACES................................................................................................................. 36
9.2 FUSE REPLACEMENT .................................................................................................................................... 36
9.3 BELT REPLACEMENT ....................................................................................................................................39
10 SERVICE............................................................................................................................................................. 42
10.1 TROUBLESHOOTING......................................................................................................................................42
10.2 REPLACEMENT PARTS.................................................................................................................................. 43
10.3 ACCESSORIES............................................................................................................................................... 44
10.3.1 Available Platforms, 46 x 76 cm (18 x 30 in).....................................................................................44
10.3.2 Flask Clamps for Universal Platforms..............................................................................................44
10.3.3 Replacement Clamp Hardware Kits..................................................................................................44
10.3.4 Carriers and Test Tubes.....................................................................................................................45
10.3.5 Optional Bases for I-26/26R..............................................................................................................45
10.3.6 Stacking Kit for I-26/26R................................................................................................................... 46
10.3.7 Optional Handles............................................................................................................................... 46
11 DRAWINGS........................................................................................................................................................ 47
11.1 CONTROL SCHEMATICS................................................................................................................................47
11.2 LIST OF DRAWINGS ......................................................................................................................................51
12 INDEX..................................................................................................................................................................52
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11
1.1 Hazard Icons
General hazard
Electrical shock hazard
1.2 Danger levels
The following danger levels are used in safety messages throughout this manual.
DANGER WARNING CAUTION ALERT
Will lead to severe injuries or death. May lead to severe injuries or death. May lead to light or moderate injuries. May lead to material damage.
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Risk of material damage
Crush warning
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1.3 Manual conventions
Depiction Meaning
1.
2.
1.4 Abbreviations
RPM VA VAC DC RTD LED
Perform these actions in the sequence described.
List
References useful information.
Revolutions per Minute Volt Amp Voltage in Alternating Current Direct Current Resistance Temperature Detector Light Emmitting Diode
I-26/26R Incubator Shakers M1324-0050 Operating Manual
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22
The I-26/26R large stackable incubator shakers are large capacity orbital shakers that utilize a triple eccentric counter-balanced drive mechanism. They provide horizontal plane rotary motion in a 2.54 cm (1 in) diameter circular orbit. A Proportional / Integral (PI) microprocessor controller with instantaneous digital feedback controls the speed over a range of 25-400 rpm.
The I-26 provides temperature control from 5C above ambient to 60C. The refrigerated I-26R assures temperature control down to 15C below ambient, with a minimum setpoint of 4C. Ambient temperature is measured one meter from the front of the unit.
The shakers may be operated either continuously or in a timed mode via a programmable timer for shaking periods of 0.1 to 99.9 hours.
For safe operation, the I-26/26R models are designed with a safety switch that automatically stops the shaker mechanism when the door is opened.
The I-26/26R is equipped with visual and audible alarms that alert the user to the following conditions:
The end of a timed run Deviations from speed setpoint Deviations from temperature setpoint Power failure Door open Unstable operating conditions (tilt switch—visual alarm only)
A wide variety of slide-out platforms can be used with the I-26/26R. Dedicated platforms and universal platforms are available for a variety of flask sizes. Test tube racks, microtiter plate holders, and test tube rack holders are also available (a universal platform is needed for all test tube racks and holders). For further information on these accessories, see Section 10.3.
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2.1 Front View
This drawing is for reference, to acquaint you with the user interface controls:
Figure 1: Front View
USER INTERFACE (display, indicator lights & keypad
)
Door is open
2.2 Specifications
These specifications assume a maximum load of 34 pounds (15.5 kg), including platforms, clamps, glassware and contents.
SHAKING Speed
Control Accuracy Indication Stroke/Orbit TEMPERATURE I-26 Range I-26R Range Control Accuracy Indication Heaters
ALARMS
Single unit: 25-400 rpm 2 or 3 units stacked: 25-250 rpm 1 rpm 3 Digit LED, in 1 rpm increments
2.5 cm (1 in)
5°C above ambient temperature to 60°C 15°C below ambient (minimum 4°C) to 60°C 0.1°C from 30-40 °C, 0.5°C over the remaining range. 3 Digit LED, in 0.1°C increments Long-life, low-watt density resistance-type heaters with high temperature thermostats Visible and audible warning indication when speed deviates more than 5 rpm or temperature more than 1°C from setpoints, and when timer has expired. Audible alarm can be muted.
PLATFORM RESTS on back of door
I-26/26R Incubator Shaker
...continued...
ON/OFF Switch
I-26/26R Incubator Shakers M1324-0050 Operating Manual
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I-26/26R Incubator Shaker
LED DISPLAY
RS 232 SETPOINT RETENTION AUTOMATIC RESTART STACKING
DRIVE
DRIVE MOTOR SAFETY
ELECTRICAL REQUIREMENTS
SAFETY REGULATORY STANDARDS CE REGULATORY STANDARDS OVERALL DIMENSIONS Width Depth (Front to Back) Height (excluding optional base) CHAMBER DIMENSIONS PLATFORM WEIGHT
FUSES
Indicates speed, temperature, running time alarm conditions, and displays readout of internal clock (actual accumulated operating time). Character height: 14.3 mm (9/16 in) Provides access for remote data logging
All setpoints and operating status are retained in non-volatile memory
Automatic restart after power is restored, indicated by flashing display
Up to three units may be stacked; the second and third units require stacking kit(s). Triple-eccentric counterbalanced drive with nine permanently lubricated ball bearings Solid-state brushless DC motor.
An independent mechanical sensing “tilt switch” shuts off the motor in an unbalanced condition. Drive Interrupt shuts off power to shaker when door opens. Acceleration/deceleration circuit prevents sudden starts and stops, minimizing both splashing and mechanical damage. 100 Volts, 50/60 Hz 120 Volts, 60 Hz 230 Volts, 50 Hz
UL61010A-1
UL61010-2-010
See DECLARATION OF CONFORMITY
Single Unit
128.3 cm (50.5 in) 77 cm (30.32 in) 70 cm (27.56 in)
86.4 cm (34 in) Wide X 58.4 cm (23 in ) Deep X 39.4 cm (15.5 in) High clearance above platform
Aluminum, 46 cm X 76 cm (18 in X 30 in) I-26: 167.8 kg (370 lbs)
I-26R: 181.5 kg (400 lbs) Two 8.0A 250V, Slo-Blo
Two Units
128.3 cm (50.5 in) 77 cm (30.32 in)
133.5 cm (52.56 in)
®
I-26: 800 VA per shaker I-26R: 1500 VA per shaker
CAN/CSA-C22.2 No 1010.1
CAN/CSA-C22.2 No 1010.2.010
Three Units
128.3 cm (50.5 in) 77 cm (30.32 in)
197 cm (77.56 in)
2.3 Certifications
The I-26 and I-26R have been tested to comply with UL and CAN/CSA electrical safety standards (see “Safety Regulatory Standards” in the specifications table). As attested in the CE Declaration of Conformity reproduced on the following page, they also conform to the appropriate EU standards.
New Brunswick Operating Manual
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I-26/26R Incubator Shakers M1324-0050 Operating Manual
11
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3.1 Inspection of Boxes
After you receive your order from Eppendorf/New Brunswick, inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to the carrier and to your local New Brunswick or Eppendorf Sales Order Department.
3.2 Packing List Verification
Verify against your packing list that you have received the correct materials.
3.3 Unpacking of Equipment
CRUSH WARNING!
Do not attempt to lift the I-26/26R by hand.
Always use a lifter or other suitable equipment when raising or handling the shaker.
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To unpack your I-26/26R, you will need the following:
Hammer Forklift or other lifting equipment to lift 168 kg (370 lb) or more Shears to cut 12.7 mm (½ in)wide plastic strapping Tool to remove 7.6 cm (3 in) metal staples
If any part of your order was damaged during shipping, is missing, or fails to operate, fill out the "Customer Feedback" online at www.nbsc.com/CustomerFeedback.aspx or call Eppendorf/New Brunswick or your distributor's service department.
New Brunswick Operating Manual
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44
4.1 Physical Location
It is essential that the instrument be situated in an area where there is sufficient space for the shaker and platform to clear walls and obstructions during operation. The surface on which the unit is placed must be smooth, level, and able to support the shaker under full load operating conditions.
CRUSH WARNING!
Do not attempt to lift the I-26/26R by hand.
Always use a lifter or other suitable equipment when raising or handling the unit.
4.2 Environment
The shaker is designed to operate optimally in the following ambient conditions:
5º to 35°C 20 to 80% Relative Humidity non-condensing
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4.3 Electrical Requirements
100, 120 or 230 VAC (depending on your selection) 800 VA for each I-26, 1500 VA for each I-26R
I-26/26R Incubator Shakers M1324-0050 Operating Manual
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4.4 Space Requirements
Figure 1a: Dimensions
DEPTH:
77 cm
30.32 in
DOOR EXTENSION:
48.4 cm
19.05 in
WIDTH: 128.3 cm
50.5 in
HEIGHT: 70 cm
27.56 in
DOOR WIDTH:
99.5 cm
39.16 in
Be sure to allow four inches (10 cm) around the shaker for proper ventilation, and 24 inches (61 cm) on the right side for service access to fan and refrigeration assemblies.
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55
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CRUSH WARNING!
Do not attempt to lift the I-26/26R by hand.
Always use a lifter or other suitable equipment when raising or handling the unit.
5.1 Tools Required for Installation
To install the I-26/26R on an optional base and/or to stack I-26/26R units, the following tools will be needed:
Number 2 Phillips head screwdriver Level, 25.4 cm (10 in) Two adjustable wrenches Forklift or other lifting equipment to lift more than 168 kg (370 lb) Metal leveling shims
II--2266//2266RR
5.2 Leveling a Single Shaker
Do NOT use feet if you are stacking shakers or adding a base.
Make sure that the shaker is placed on a level surface and that all four feet are solidly on the surface. If the shaker is not level, adjust the feet as needed to achieve leveling:
1. Immobilize the top lock nut against the unit with one wrench whenever you adjust the foot, to keep the threaded stud from falling out.
Figure 2: Adjustable Foot
BOTTOM OF
UNIT
FOOT
LOCK NUT
FLATS FOR WRENCH
I-26/26R Incubator Shakers M1324-0050 Operating Manual
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2. With a second wrench against the flats of the threaded stud, just above the foot: rotate clockwise to lower the foot or counter-clockwise to raise the foot.
3. Place a level on the top of the unit.
4. If necessary, make further adjustments by repeating all steps until the unit is level.
5. Fully load the shaker, and do a test run at normal speed. Make additional leveling adjustments if necessary.
The maximum foot height adjustment is ~ 6.4 mm (¼ in). If a higher adjustment is required, you will need to add metal shims.
5.3 Adding a Base
There are three bases available for your I-26/26R shaker(s). The height of the base depends on whether you are installing one unit on the base, or stacking two or three units:
Table 1: Optional Bases
Number of Shakers Base Height
43.2 cm (17 in)
1
2 stacked 33.0 cm (13 in) 3 stacked 10.2 cm (4 in)
One 10.2 cm (4 in) base & one 33.0 cm (13 in) base,
bolted together at the factory
5.4 Preparing the Optional Base
1. Place the base on a sturdy (capable of bearing the weight of the combined base shakers and shaker contents), level surface, making sure that all four corners are solidly on the surface.
2. If the base is not level, place metal shims as needed under the base until it is level.
3. If you are mounting a single shaker, use the 43.2 cm (17 in) base as shown in Figure 3b. Bolt the shaker to the base using the hardware provided.
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Figure 3a: Optional Short Base, 10.2 cm (4 in)
REAR CORNER BRACE
10.2 cm (4 in) BASE
FRONT SIDE BRACE
Figure 3b: Optional Tall Base, 43.2 cm (17in)
BASE MATING SUPPORTS
33 cm (13 in) BASE
4. As shown in Figures 3a-b, depending on the height of your base, install the front side braces and rear corner braces on the base with the hardware provided, matched to the holes in the base.
I-26/26R Incubator Shakers M1324-0050 Operating Manual
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5.5 Mounting the I-26/26R on the Optional Base
1. Using a forklift or lifter, raise the I-26/26R so that its back end is tilted toward the rear of the base.
2. Remove all six feet from the bottom of the shaker. They will not be needed for stacking, but you may want to keep them for future use.
3. With two assistants, each holding the shaker on one side, lower the unit onto the base, rear panel first. Slowly and gently remove the forklift or lifter and lower the front of the unit onto the base by hand.
Optional handles are available to facilitate maneuvering the shaker onto the
base. See Section 10.3.7 for details.
Figure 4: Mounting Shaker on Base
4. Using the ½-13 x 1¼-inch Allen head screws, ½-inch lock washers, and washers provided, secure the rear of the I-26/26R to the corner braces in the base. With the remaining ½-13 x 1¼-inch Allen head screws, ½-inch lock washers, and washers, attach the front corner braces to the unit.
5. Attach the front corner braces to the base using the two sets of ½-13 x 1¼-inch Allen head screws, lock washers, and washers provided, and secure as shown in Figure 5.
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