Eppendorf Galaxy Gas Analyzer User Manual

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Instructions

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Galaxy® CO₂ Sensor SetupGalaxy® CO2 Sensor Setup

see

on p.Fig.Tab.p

nstructions

March 31, 2012

New Brunswick CO2 Incubators

Galaxy® CO

Sensor Setup

Instructions

CO2SENS-0060

Revision A

New Brunswick reserves the right to change information in this document without notice. Updates to information in this document reflect our commitment to continuing product development and improvement.

Trademarks

Eppendorf® is a registered trademark of Eppendorf AG, Germany.

New Brunswick and the New Brunswick Logo™ are trademarks of Eppendorf AG, Germany.

Galaxy

is a registered trademark of New Brunswick Scientific Co., Inc., USA.

WAGO® is a registered trademark of Kontakttechnic GmbH & CO. KG, Germany.

Pozidriv

is a registered trademark of Phillips Screw Company, USA.

Trademarks are not marked in all cases with ™ or ® in this manual.

New Brunswick has attempted to identify the ownership of all trademarks from public records. Any omissions or errors are unintentional.

April 25, 2012 Revision A CO2SENS-0060

Galaxy® CO2 Sensor Setup — Instructions

1 Galaxy 170 R / 48 R 120 V / 230 V CO2 Incubators

1.1 K1 CO

Parts Supplied

1.3 m Tube, Silicone Rubber, Clear

12 Cable Tie, 2.5 x 100 Long

Sensor update kit retro fit instructions

1CO

1 Porous Sensor Cover, Sintered 1 Bracket Strain Relief 6 Washer, M3 Spring, A2 Stainless Steel 3 M3 Washer 3M3 Nut 3 M3 detector spacer 3.18 mm ID x 7 mm 2 Cable Tie, 2.5 x 100 Long 1Nut M4 1 Spacer 9.53 x 12.7 Long 1 Cable Tie Mount 1 M4 x 20 Pozidriv Screw 1 Spring, Tube Support 1 Solenoid Valve Assembly 24 VDC 1 Equipment Tray Cover Panel

2 M4 Washers 2

1 Bracket Self Adhesive Solenoid Valve 2 Self Adhesive Pads 1 IPA Cleaning Pad 1 Galaxy R-Plus Board 'Two' 2 EPROM ( Updated For Auto-Zero 1 off each model)

Sensor with Flying Leads and Connector Block

M4 x 6 Pozidriv

Screws

Galaxy 170 R / 48 R 120 V / 230 V CO

Incubators

Galaxy® CO2 Sensor Setup — Instructions

Tools required/recommended

Large sized Pozidriv screwdriver – for Equipment Tray and cover screws Small sized Pozidriv screwdriver – for 170 R Expansion PCB screws Side-Cutters – for cutting cable zip ties

5.5 mm nutspinner – for CO

5 mm nutspinner – for Expansion Board RS232 9 Way ‘D’ Connector fixing posts T-bar torque limiter screw driver with attachment to fit M3 Hex EPROM extractor tool or Small flat bladed screwdriver – to remove EPROM ESD Wrist strap

1.2 Removing existing parts

Sensor M3 fixing nuts

1. Power down the Incubator and completely disconnect from the Mains/Power Supply. Open the outer and Inner Door (option on 48 R) then remove the white porous cover on the CO2 Sensor from inside the chamber of the incubator (dashed red circle in image below).

Galaxy 170 R / 48 R 120 V / 230 V CO

2. Rear cover removal:

170 R – Remove the rear access panel by unplugging the access port plug and then

unscrewing the 8 x Pozidriv head screws with washers (see all screws and plug in dashed red circles in image below).

NOTE: Protect top face of equipment tray with tape.

48 R – Remove the CO

with washers (see all screws in dashed red circles in image below).

Sensor access panel by unscrewing the 4 x Pozidriv head screws

48 R – Cover slots along the top face of the power supply box with tape. Remove after retro fit is complete.

Galaxy® CO2 Sensor Setup — Instructions

3. With the access panel removed, locate the insulation block covering the CO2 Sensor and carefully remove the block (shown in yellow dashed boxes in images below) and place to one side for refitting later. The rear of the CO

Sensor will now be visible.

Galaxy 170 R / 48 R 120 V / 230 V CO

4. Remove the existing CO unplug the Auto-Zero port tubing leading from the pump (see dashed yellow circles on image to the left below). Unscrew the 3 x M3 securing nuts and washers, and place to the side for refitting (only one nylon washer will be reused).

5. Equipment Tray and 48 R Top Cover:

170 R – Open the rear equipment tray by unscrewing the 2 x large Pozidriv head screws as shown, reference top left-hand image on page 6 and then remove the cover plate shown arrowed on top right-hand image page 6 by unscrewing the 4 x Pozidriv head screws. Discard the old plate after removal. Save screws for further use.

Sensor by disconnecting the 5 way Molex connector and then

Incubators

Galaxy® CO2 Sensor Setup — Instructions

48 R – Remove the Top Cover by unscrewing the 4 x large Pozidriv head screws. Pull the Top Cover forward and lift upwards. The wiring harness to the LCD and Keypad is not long enough to place the top cover at the side of the Incubator.

Galaxy 170 R / 48 R 120 V / 230 V CO

Carefully position top cover.

Galaxy® CO2 Sensor Setup — Instructions

Carefully snip zip ties.

Secure top cover as shown.

Galaxy 170 R / 48 R 120 V / 230 V CO

Incubators

Re-secure top cover when complete.

NOTE: Now procede to step 7 for 48 R.

6. On the 170 R locate the Auto-Zero Pump and disconnect the tubing leading to the CO Sensor from the Auto-Zero Pump (see “Pump Out” in diagram below). The tubing is fixed to the pump barbs using cable zip ties. Trace the tubing back to where it enters the Cover Plate access hole area and cut the tubing back to this point using the side cutters; a new length of tubing will be fitted later.

Incubators

Galaxy® CO2 Sensor Setup — Instructions

7. Existing Expansion PCB removal:

170 R – Using the diagram below with points highlighted in yellow:

1. Disconnect the 40 way Ribbon cable to Board 2 Expansion PCB.

2. Remove the 3 mm Nut securing the Earth tag to the Tray.

3. Unscrew the 2 x RS232 Hex head fixing posts.

4. Disconnect the wiring for the Live In and the Base Element Live Out as well as the wiring

for any other options fitted. Take note of locations if unsure as this will help to reconnect the replacement Expansion PCB.

5. Remove the 4 x Pozidriv Head Securing Screws.

Galaxy 170 R / 48 R 120 V / 230 V CO

48 R – Using the diagram below with points highlighted in yellow:

1. Disconnect the 40 way Ribbon cable to Board 2 Expansion PCB.

2. Unscrew the 2 x RS232 Hex head fixing posts.

3. Disconnect the wiring for any options fitted. Take note of locations if unsure as this will

help to reconnect the replacement Expansion PCB.

4. Lift up carefully from the PCB Support Pillars.

Galaxy® CO2 Sensor Setup — Instructions

Fitting the new Parts

8. Solenoid Valve fitting:

170 R - Mount the Solenoid Valve to the new cover plate using the fixings supplied and fit the

new cover plate in the position shown in the image below using the 4 x Pozidriv head screws which were kept aside earlier. Route the Red/Black wires from the Solenoid Valve along existing cable route to the Expansion PCB, secure with zip ties. The tubing will be connected when fitting the new CO2 Sensor.

Galaxy 170 R / 48 R 120 V / 230 V CO

Incubators

48 R – Fit the 48 R Solenoid Valve mounting bracket with self adhesive backing to the equipment tray in the position shown in the image below.

Screw Valve to bracket using screws supplied and route the Red/Black wiring for the Pump back to the expansion PCB area. The tubing will be connected when fitting the new CO Sensor.

9. Cable zip tie all wires in place to secure to tray.

Incubators

Galaxy® CO2 Sensor Setup — Instructions

10. Replacement Expansion PCB fitting:

170 R - Refitting is a reversal of removal. Follow the wiring diagram and image below.

Galaxy 170 R / 48 R 120 V / 230 V CO

48 R - Refitting is a reversal of removal. Follow the wiring diagram and image below.

Galaxy® CO2 Sensor Setup — Instructions

11. 170 R CO2 Sensor fitting

Unpack the new CO2 Sensor and fix in place with the bracket supplied and the original nuts and washers and new spring washers but only reuse one of the original nylon washers in the location shown in the image below. Fit the spring washers Tighten the M3 fixing nuts to 0.4 Nm (Newton meters).

Galaxy 170 R / 48 R 120 V / 230 V CO

Incubators

12. Route the Auto-Zero inlet tubing from the CO back cover to the Solenoid Valve then connect the tubing to the top barb of the Solenoid Valve as shown on top image page 12.

Sensor down between the insulation and the

Incubators

Galaxy® CO2 Sensor Setup — Instructions

13. Route the tube from the Auto-Zero Pump “Out” (see Pump diagram at the bottom of Page 7) to the bottom barb of the Solenoid Valve shown in the image above.

NOTE: Air flow must follow the direction of flow arrows on Auto-Zero Pump and CO Valve.

Air Flow: Hepa filter..Air In….Auto-Zero pump..Air In / Air Out…….CO In / Air Out…..CO2 Sensor Assembly…Air In.

Solenoid

Solenoid Valve…..Air

NOTE: If the elbow becomes loose when fitting tube, it must be resealed with elbow secured

Galaxy 170 R / 48 R 120 V / 230 V CO

in direction shown. If the elbow becomes loose, the integrity of the CO compromised.

14. Cut the old Pump tubing in line with the glass fiber insulation and trim the CO harness in line with the glass fibre insulation and strip and twist 7 mm from the end.

15. Connect the existing wiring harness to the CO and right-hand image, page 13). Ensure you have stripped 7 mm of insulation from the existing harness wires and twisted the strands before connecting them.

NOTE: Green on the existing harness connects to Brown on the new CO2 Sensor assembly harness.

upgrade may be

Sensor wiring

Sensor connecting block (as shown on top left

Galaxy® CO2 Sensor Setup — Instructions

Galaxy 170 R / 48 R 120 V / 230 V CO

Incubators

16. 48 R CO2 Sensor fitting:

NOTE for 48 R: The replacement CO

this tubing with spring and use the existing tubing already fitted to the Incubator. Unpack the new CO and new spring washers but only reuse one of the original nylon washers in the location shown in the image below. Refit the spring over the existing tubing. Fit the spring washers Tighten the M3 fixing nuts to 0.4 Nm (Newton Meters).

Sensor and fix in place with the bracket supplied and the original nuts and washers

Sensor has tubing pre fitted for the 170 R. Remove

Incubators

Galaxy® CO2 Sensor Setup — Instructions

17. Disconnect the tube from the Auto-Zero Pump “Out” (see Pump diagram at bottom of page 7) and connect to the “Pump Out to CO image below). Using the new tubing supplied, cut a length and connect to the “Pump In” barb of the Solenoid Valve shown in the image below.

Sensor” barb of the Solenoid Valve (as shown in the

NOTE: If the elbow becomes loose when fitting tube, it must be resealed and secured. If the elbow becomes loose, the integrity of the CO2 upgrade may be compromised.

Galaxy 170 R / 48 R 120 V / 230 V CO

Note: Air flow must follow the direction of flow arrows on Auto-Zero Pump and CO Valve.

Air Flow: Hepa filter..Air In….Auto-Zero pump..Air In / Air Out…….CO In / Air Out…..CO

18. Connect the existing wiring harness to the CO and right-hand image, page 15). Ensure you have stripped 7 mm of insulation from the existing harness wires and twisted the strands before connecting them.

NOTE: Green on the existing harness connects to Brown on the new CO2 Sensor assembly harness.

Sensor Assembly…Air In.

Solenoid

Solenoid Valve…..Air

Sensor connecting block (as shown on top left

Galaxy® CO2 Sensor Setup — Instructions

Galaxy 170 R / 48 R 120 V / 230 V CO

Incubators

19. Refit the insulation block and trim a small pocket in the insulation to locate the connecting block (as shown in yellow dashed box in image below).

20. Refit the rear access panel using the 8 x Pozidriv head screws with washers and refit the Access Port Plug for the 170 R and the 4 x Pozidriv head screws with washers on the 48 R (see drawings on Page 4).

21. Fit the new porous cover supplied to the CO body until it touches the back wall of the chamber.

NOTE: It may be difficult to push on so use the palm of the hand to push back.

Sensor by sliding it over the brass measuring

Incubators

Galaxy® CO2 Sensor Setup — Instructions

22. EPROM Replacement

NOTE: To avoid static damage, take anti-static precautions and avoid touching the legs of the

new device during fitting.

We recommended that an extractor tool is used to remove the existing EPROM. If an extractor is not available then insert a small flat bladed screwdriver under one end of the IC and gently ease it upwards, then insert the screwdriver under the other end and ease that end up, repeat until the IC is free of the socket.

Before insertion of the replacement EPROM make sure you observe the proper orientation of the device. Orientation is achieved by ensuring the notch is aligned with the notch in the IC Socket of each device (see Notch circled in image below).

When fitting the EPROM in the socket ensure all legs are correctly aligned and none are bent when inserted.

Galaxy 170 R / 48 R 120 V / 230 V CO

23. Equipment Tray (170 R) and Top Cover (48 R) refitting

170 R – Close the rear equipment tray and refit the 2 x large Pozidriv head screws.

48 R – Carefully refit the Top Cover using the 4 x large Pozidriv head screws taking care not

to trap any wires or tubing between the Top Cover and Equipment Tray.

1.3 To complete the installation the new CO2 Factors must be entered as follows

1. Power up the Incubator and program the CO2 level to 0.0%.

2. Enter the Engineering menu by pressing ‘DIAG’ then ‘ENG’ then enter the access code of ‘1973’ and press ‘ENTER’.

3. Select and enter the ‘CO2 Auto-Zero’ screen and adjust the Factor to the Zero Factor provided by using the ¶ and · arrow keys and pressing ENTER.

4. Enter the ‘CO2 AUTOGAIN’ screen and select CO2 GAIN FACTOR by using the » key, enter the Gain Factor provided by using the ¶ and · arrow keys and pressing ENTER. ‘EXIT’ this screen and ‘EXIT’ the Engineering Menu to return to the front screen.

5. Humidify incubator.

6. Allow chamber environment to homogenize at 37 deg C set point. Typically 8 hours.

7. Program CO

8. Allow CO

9. Perform Auto-Zero cycle.

10. Perform calibration procedure.

to stabilize.

at 5 %.

Galaxy® CO2 Sensor Setup — Instructions

2 Galaxy 170 S 230 V / 120 V CO2 Incubators

2.1 K2 CO

Parts Supplied

1.3 m Tube, Silicone Rubber, Clear

20 Cable Tie, 2.5 x 100 Long

0.6 m Brown 18 AWG UL1015 105 DegC with Female Crimp Spade Terminal

Sensor update kit retro fit instructions

1CO

2 M4 Washers 2 M4 x 6 Pozidriv Screws

1 PCB Assembly with bracket plate 3 M3 Washers 3 M3 x 12 Cheese Head, Stainless 3 Detector spacer, 3.18 mm ID x 7 1 5 V Miniature Diaphragm Pump

4 M3 x 6 Pozidriv Screw 6 Large Cable Tie, 3.5 mm . 300 mm long 6 Large Cable Tie Base, 27 mm x 27 mm

Sensor with Flying Leads and Connector Block

Galaxy 170 S 230 V / 120 V CO

Incubators

Galaxy® CO2 Sensor Setup — Instructions

Tools required/recommended

Large sized Pozidriv screwdriver – for Equipment Tray and cover screws

Small flat bladed screwdriver – for removing and fitting wires to WAGO Side-Cutters – for cutting cable zip ties

5.5 mm nutspinner – for CO

Torque Limiting Screwdriver with attachment to fit M3 5.5 m Hex – for fitting M3 Hex fixing nuts on new CO

ESD Wrist strap

Sensor M3 fixing nuts

2.2 Removing existing parts

1. Power down the Incubator and completely disconnect from the Mains/Power Supply. Open the outer and Inner Door then remove the white porous cover on the CO2 Sensor from inside the chamber of the incubator (dashed red circle in image below).

block

Sensor

2. Remove the rear access panel by unplugging the access port plug and then unscrewing the 8

Galaxy 170 S 230 V / 120 V CO

x Pozidriv head screws with washers (see all screws and plug in dashed red circles in image below).

NOTE: Protect top face of equipment tray with tape.

3. With the access panel removed, locate the insulation block covering the CO2 Sensor and carefully remove the block (shown in yellow dashed box on top image of page 19) and place to one side for refitting later. The rear of the CO

Sensor will now be visible.

Galaxy® CO2 Sensor Setup — Instructions

4. Remove the existing CO2 Sensor by disconnecting the 5 way Molex connector and then unplug the Auto-Zero port tubing leading from the pump (see dashed yellow circles on image to the left below). Unscrew the 3 x M3 securing nuts and place to the side for refitting (only one nylon washer will be reused).

Galaxy 170 S 230 V / 120 V CO

5. Open the rear equipment tray by unscrewing the 2 x large Pozidriv head screws and then remove the cover plate shown arrowed in image on page 20 by unscrewing the 4 x Pozidriv head screws. Discard the old plate after removal.

Incubators

Galaxy® CO2 Sensor Setup — Instructions

6. Locate the Auto-Zero Pump and disconnect the tubing leading to the CO Auto-Zero Pump (see “Pump Out” in diagram below). The tubing is fixed to the pump barbs using cable zip ties. Trace the tubing back to where it enters the Cover Plate access hole area and cut the tubing back to this point using the side cutters; a new length of tubing will be fitted later. Also disconnect the other tube into the Pump from the Air Zero Inlet Filter.

Galaxy 170 S 230 V / 120 V CO

7. Trace the Pump wiring back to the WAGO connector block (as shown on top image, page 21) and use side cutters to carefully cut all cable zip ties which are securing the wiring as you go.

NOTE: It is very easy to damage wires when cutting cable zip ties so take care when doing this. Use a small flat bladed screwdriver to release the Red and Black wiring from the WAGO connecting block and withdraw the existing pump from its holder (see locations for Red and Black wiring, reference top image, page 21).

Sensor from the

Galaxy® CO2 Sensor Setup — Instructions

WAGO connecting block with Auto-Zero Pump connections highlighted

Fitting the new Parts

8. Fit the new Pump with Diode supplied in the Kit and cable zip tie the wiring down in the same locations as the old pump. Trim the wires to length then strip and twist 7 mm for connecting back into the WAGO connector block.

Galaxy 170 S 230 V / 120 V CO

Incubators

9. The PCB can now be positioned in the right-hand corner of the tray but it may be necessary to relocate the 3 x Connectors for the Door Harness to clear room for the Auto-Zero Solenoid Valve PCB/bracket (see blue arrows in image on following page). The Harness connectors are cable zip tied to adhesive bases which are fixed down to the tray, there may also be other cable zip ties and bases securing the wiring to the tray (see locations in dashed yellow circles in image on following page.)

10. Cut all cable zip ties and relocate the connectors with the cable zip ties and bases provided in the kit, leaving enough clearance for the Solenoid Check Valve PCB/bracket. After the Door Harness connections have been moved the bracket mounting points will be visible.

Incubators

Galaxy® CO2 Sensor Setup — Instructions

Door Harness Connectors

Solenoid Pump Valve PCB bracket mounting points

Galaxy 170 S 230 V / 120 V CO

11. Mount the PCB/bracket in the tray as shown in the image below using the screws supplied to secure in position then route the wiring along the back of the Equipment Tray.

Galaxy® CO2 Sensor Setup — Instructions

12. Route the Red/Black wires from the PCB to the Auto-Zero Pump connections on the WAGO connecting block. Use the small flat bladed screwdriver to insert the wires into the right-hand side of the WAGO connector block making a piggyback connection where the existing Red/ Black wires are connected. Take care when inserting the wires that the existing wires still have a good connection and most importantly ensure correct orientation of the wires.

13. Mount the Solenoid Valve to the new cover plate using the fixings supplied and fit the new cover plate in the position shown in the image below using the 4 x Pozidriv head screws which were kept aside earlier. The tubing will be connected when fitting the new CO

Sensor.

Galaxy 170 S 230 V / 120 V CO

Incubators

Galaxy® CO2 Sensor Setup — Instructions

14. Connect the Solenoid Valve Neutral (Black wire with Crimp Female Spade) to a spare location on the Neutral (Black/Blue) location on the Mains Terminal Block.

15. Connect the Valve Live (thin Red wire) to the left location on the J3 connector on the PCB (as shown in the image below). Push down on Orange tab to open these lever clamp connectors. Connect the stripped end of the Live In (thick Brown wire) to the right side of the J3 connector (as shown in the image below).

Galaxy 170 S 230 V / 120 V CO

16. Route the Live (thick Brown wire with Female Spade Crimp) along the rear of the tray and then to a spare location on the Live (Brown/Red) part of the Mains Terminal Block (as shown in top images on page 25).

Galaxy® CO2 Sensor Setup — Instructions

17. Cable zip tie all wires in place to secure to tray.

Galaxy 170 S 230 V / 120 V CO

18. Unpack the new CO and washers and new spring washers but only reuse one of the original nylon washers in the location shown in the image below. Fit a spring washer then M3 Washer with M3 Nut to each of the studs through the bracket, reuse the Nylon washer with M3 Washer and Nut on the other stud. Tighten the M3 fixing nuts to the recommended 0.4FT/LBS (Foot-Pounds).

Sensor and fix in place with the bracket supplied and the original nuts

Incubators

Galaxy® CO2 Sensor Setup — Instructions

19. Route the Auto-Zero inlet tubing from the CO2 Sensor (pre fitted to the new Sensor) down between the insulation and the back cover to the Solenoid Valve and connect the tubing to the top barb of the Solenoid Valve as shown in the image below.

Galaxy 170 S 230 V / 120 V CO

20. Route the tubing supplied from the new Auto-Zero Pump “Out” to the bottom barb of the Solenoid Valve shown in the image above and reconnect the “Pump In – from Inlet filter” tubing.to the other barb of the new Auto-Zero Pump.

NOTE: Air flow must follow the direction of flow arrows on Auto-Zero Pump and CO Valve.

Air Flow: Hepa filter..Air In….Auto-Zero pump..Air In / Air Out…….CO In / Air Out…..CO2 Sensor Assembly…Air In.

NOTE: If the elbow becomes loose when fitting tube, it must be resealed with elbow secured in direction shown. If the elbow becomes loose, the integrity of the CO compromised

Solenoid

Solenoid Valve…..Air

upgrade may be

Galaxy® CO2 Sensor Setup — Instructions

21. Cut the old Pump tubing in line with the glass fibre insulation and also trim the CO2 Sensor wiring harness to this point then strip and twist 7 mm from the end.

Galaxy 170 S 230 V / 120 V CO

22. Connect the existing wiring harness to the CO right-hand images below). Ensure you have stripped 7 mm of insulation from the existing harness wires and twisted the strands before connecting them.

NOTE: Green on the existing harness connects to Brown on the new CO2 Sensor assembly harness.

Sensor connecting block (as shown in left and

Incubators

Galaxy® CO2 Sensor Setup — Instructions

23. Refit the insulation block and trim a small pocket in the insulation to locate the connecting block (as shown in yellow dashed box in image below).

24. Refit the rear access panel using the 8 x Pozidriv head screws with washers and refit the Access Port Plug.

25. Fit the new porous cover supplied to the CO body until it touches the back wall of the chamber.

NOTE: It may be difficult to push on so use the palm of the hand to push back.

26. Close the rear equipment tray and refit the 2 x large Pozidriv head screws.

Sensor by sliding it over the brass measuring

Galaxy 170 S 230 V / 120 V CO

2.3 To complete the installation the new CO2 Factors must be entered as follows

1. Power up the Incubator and program the CO2 level to 0.0%.

2. Enter the ENGINEERING MENU by pressing the up º and down » arrow keys simultaneously.

Hint!

To navigate the menu system use the arrow keys, press to select each screen and to exit

press .

3. Enter the CO2 rEF. screen in the Engineering Menu and enter the Factor provided by using the º and » keys then press to store the change. Press to exit back to the Engineering Menu.

4. Navigate up through the ENGINEERING Menu using the º key to the CO2 SP.N screen.

5. Enter the CO2 SP.N screen, then enter the Span Factor provided in exactly the same way as entering the Ref Factor.

6. Exit the screen by pressing and exit the Engineering Menu by pressing to return to the root screen.

7. Humidify incubator.

8. Allow chamber environment to homogenize at 37 deg C set point. Typically 8 hours.

9. Program CO

10. Allow CO

11. Perform Auto-Zero cycle.

12. Perform calibration procedure.

at 5 %.

to stabilize.

Galaxy® CO2 Sensor Setup — Instructions

3 Galaxy 48 S / 14 S 120 V & 230 V CO2 Incubators

3.1 K5 CO

Parts Supplied

Sensor update kit retro fit instructions

1CO

1 Porous Sensor Cover, Sintered 1 Bracket Strain Relief 6 Washer, M3 Spring, A2 Stainless Steel 3 M3 Washer 6 Cable Tie, 2.5 x 100 Long 1M4 Nut 1 Cable Tie Mount 1 Spacer 9.53 x 12.7 long 1 M4 x 20 Pozidriv Screw 3M3 Nut 3 M3 detector spacer 3.18 mm ID x 7 mm 1 Black Cap

Sensor with Flying Leads and Connector Block

Galaxy 48 S / 14 S 120 V & 230 V CO

Incubators

Tools required/recommended

Large sized Pozidriv screwdriver – for Rear cover screws

5.5 mm nutspinner – for CO

Torque Limiting Screwdriver with attachment to fit M3 5.5 m Hex – for fitting M3 Hex fixing nuts on new CO

ESD Wrist strap

Sensor M3 Hex fixing nuts

3.2 Removing existing Parts

1. Power down the Incubator and completely disconnect from the Mains/Power Supply. Open the outer Door (and Inner Door if fitted) then remove the white porous cover on the CO2 Sensor from inside the chamber of the incubator (dashed red circle in image below).

Sensor

Incubators

Galaxy® CO2 Sensor Setup — Instructions

2. Rear cover removal:

48 S – Remove the CO

with washers (see all screws in dashed red circles in top left-hand image below).

14 S – Remove the rear access panel by unscrewing the 6 x Pozidriv head screws with washers (see all screws in dashed red circles in top right-hand image below). The top two screws secure the top cover.

Sensor access panel by unscrewing the 4 x Pozidriv head screws

3. With the access panel removed, locate the insulation block covering the CO carefully remove the block (shown in yellow dashed boxes in images below) and place to one side for refitting later. The rear of the CO

Galaxy 48 S / 14 S 120 V & 230 V CO

4. Remove the existing CO unscrew the 3 x M3 securing nuts and place all the fixings to the side for refitting later (only one nylon washer will be reused).

Sensor and

Sensor will now be visible.

Sensor by disconnecting the 5 way Molex connector and then

Galaxy® CO2 Sensor Setup — Instructions

Fitting the new CO2 Sensor

5. 48 S CO

Unpack the new CO

NOTE: fix bracket with harness relief at bottom for the 48 S.

Fit a spring washer then M3 Washer with M3 Nut to each of the studs through the bracket, reuse the Nylon washer with M3 Washer and Nut on the other stud. Tighten the M3 fixing nuts to the recommended 0.4FT/LBS (Foot-Pounds).

14 S CO

Unpack the new CO

NOTE: fix bracket with the harness relief at the top for the 14 S.

Sensor fitting

Sensor and fix in place with the bracket supplied.

Galaxy 48 S / 14 S 120 V & 230 V CO

Incubators

Galaxy® CO2 Sensor Setup — Instructions

NOTE: Large slotted screw replaced with brass straight barbed connector and sealing black cap.

Photograph "A"

Photograph "B"

Galaxy 48 S / 14 S 120 V & 230 V CO

Galaxy® CO2 Sensor Setup — Instructions

Galaxy 14 with O2 option

Galaxy 48 S / 14 S 120 V & 230 V CO

A Illustrates O

inlet connector assembly

NOTE: O

obstruction.

6. Connect the existing wiring harness to the CO and right-hand image, page 34). Ensure you have stripped 7 mm of insulation from the existing harness wires and twisted the strands before connecting them.

NOTE: Green on the existing harness connects to Brown on the new CO2 Sensor assembly harness.

inlet connector assembly is adjacent to sensor bracket, but does not cause an

Sensor connecting block (as shown on top left

Incubators

Galaxy® CO2 Sensor Setup — Instructions

7. Refit the insulation block and trim a small pocket in the insulation to locate the connecting block (as shown in yellow dashed box in image below).

Galaxy 48 S / 14 S 120 V & 230 V CO

8. Refit the rear access panel using the 4 x Pozidriv head screws with washers on the 48 S and 6 x Pozidriv head screws with washers for the 14 S (see Fig. on p. 30).

9. Fit the new porous cover supplied to the CO2 Sensor by sliding it over the brass measuring body until it touches the back wall of the chamber.

NOTE: It may be difficult to push on so use the palm of the hand to push back.

Galaxy® CO2 Sensor Setup — Instructions

3.3 To complete the installation the new CO2 Factors must be entered as follows

1. Power up the Incubator and program the CO2 level to 0.0%.

2. Enter the ENGINEERING MENU by pressing the up º and down » arrow keys simultaneously.

To navigate the menu system use the arrow keys, press ENTER to select each screen and to exit

Hint!

press .

3. Enter the CO2 rEF. screen in the Engineering Menu and enter the Factor provided by using the º and » keys then press ENTER to store the change. Press to exit back to the Engineering Menu.

4. Navigate up through the ENGINEERING Menu using the º key to the CO2 SP.N screen.

5. Enter the CO2 SP.N screen, then enter the Span Factor provided in exactly the same way as entering the Ref Factor. Exit this screen by pressing and exit the Engineering Menu by pressing to return to the root screen.

6. Humidify incubator.

7. Allow chamber environment to homogenize at 37 deg C set point. Typically 8 hours.

8. Program CO

9. Allow CO

10. Perform Auto-Zero cycle.

11. Perform calibration procedure.

at 5 %.

to stabilize.

Galaxy 48 S / 14 S 120 V & 230 V CO

Incubators

Galaxy® CO2 Sensor Setup — Instructions

Your local distributor for New Brunswick products: www.nbsc.com/ContactUs

Eppendorf AG · 22331 Hamburg · Germany · Tel: +49 40 538 01-0 · Fax: +49 40 538 01-556 · E-mail: eppendorf@eppendorf.com

New Brunswick Scientiﬁc Europe B.V. · Nijmegen · The Netherlands · Tel: +31 (0) 24 3717 600 · Email: europe@nbsbv.nl

Eppendorf North America, Inc. · Hauppauge, N.Y. USA · Tel: +1 516 334 7500 · +1 800 645 3050 · E-mail: info@eppendorf.com

Application Support Europe, International: Tel: +49 1803 666 789 · E-mail: support@eppendorf.com

North America: Tel: +1 800 645 3050 menu option 2 · E-mail: techserv@eppendorf.com

Asia Paciﬁc: Tel: +603 8023 6869 · E-mail: support_asiapaciﬁc@eppendorf.com

New Brunswick Scientiﬁc · 175 Freshwater Blvd., Enﬁeld, CT 06082-4444 · USA

Eppendorf Galaxy Gas Analyzer User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual

1 Galaxy 170 R / 48 R 120 V / 230 V CO2 Incubators

1.2 Removing existing parts

1.3 To complete the installation the new CO2 Factors must be entered as follows

2 Galaxy 170 S 230 V / 120 V CO2 Incubators

2.2 Removing existing parts

2.3 To complete the installation the new CO2 Factors must be entered as follows

3 Galaxy 48 S / 14 S 120 V & 230 V CO2 Incubators

3.2 Removing existing Parts

3.3 To complete the installation the new CO2 Factors must be entered as follows