nualR/170 S CO2 Incubators
EN) manual
New Brunswick™
Galaxy
Operating manual
®
170 R/170 S CO2 Incubators
Copyright
Copyright © 2014 Eppendorf AG, Germany. No part of this publication may be reproduced without the
prior permission of the copyright owner.
The company reserves the right to change information in this document without notice. Updates to
information in this document reflect our commitment to continuing product development and
improvement.
Trademarks
®
Eppendorf
and the Eppendorf logo are registered trademarks of Eppendorf AG, Germany.
New Brunswick™ and the New Brunswick™ logo are trademarks of Eppendorf AG, Germany.
®
Galaxy
is a registered trademark of Eppendorf, Inc., USA.
Microsoft
®
and Excel® are either registered trademarks or trademarks of Microsoft Corporation in the
United States and/or other countries.
®
Trademarks are not marked in all cases with ™ or
in this manual.
Eppendorf has attempted to identify the ownership of all trademarks from public records. Any omissions or
errors are unintentional.
6710900.017-00/012014
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Hazard symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Degrees of danger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Table of contents
2.1.1 Galaxy 170 R/170 S CO
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.1 Inspection of boxes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.2.2 Packing list verification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.1 Control system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.2 Direct heating system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.3 Infrared sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.4 Controlled humidity tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.3.5 Seamless chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.3.6 Standard features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.3.7 Multiple options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.3.8 Two-level alarm system. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.4 Stacking devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.5 Optional equipment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2
3
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.3.1 Description of ATEX Guideline (94/9EC) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.5.1 Personal injury and damage to device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.1 Utilities requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.2 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
4.3 Unpacking the incubator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.4 Initial setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.5 Making connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5 Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.1 Preparing for operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.2 Using the humidity tray. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.3 Operation for optional features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
6 Operating controls and function for Galaxy 170 R Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
6.1 170 R control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
Table of contents
Galaxy
4
English (EN)
®
170 R/170 S CO2 Incubators
6.2 Programming . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.2.1 Temperature and CO
level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
2
6.2.2 User access code. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.2.3 Removing user access code . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
6.3 Referencing the CO
sensor with Auto-Zero. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
2
6.4 USER settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
6.4.1 SET DATE AND TIME . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.4.2 AUDIBLE ALARM VOLUME ADJUST . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
6.4.3 PROGRAMMABLE CO
AUTOZERO. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
2
6.4.4 DATALOGGER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.4.5 POWER FREQUENCY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.4.6 DISABLE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.4.7 DISINFECTION (optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
6.5 DATALOGGER screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.5.1 ALARM EVENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
6.5.2 TEMPERATURE GRAPH + DOOR OPEN BAR CHART . . . . . . . . . . . . . . . . . . . . . . . . . 37
6.5.3 CO
GRAPH + DOOR OPEN BAR CHART . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
2
6.5.4 DIAGNOSTIC CHAMBER ELEMENT GRAPH . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6.5.5 DIAGNOSTIC DOOR GRAPH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.5.6 DIAGNOSTIC DOOR ELEMENT GRAPH. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.5.7 RESTART GRAPHIC RECORD. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.6 CHAMBER ALARMS menu screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
6.6.1 Chamber alarm system function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
6.7 DIAGNOSTICS menu screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.8 HELP MENU screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
7 Operating controls and function for Galaxy 170 S Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7.1 170 S control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
7.2 Setting temperature and CO
. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
2
7.3 Programming the alarm system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
7.3.1 Setting the high and low temperature alarms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
7.3.2 Setting the CO
high and low alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
2
7.3.3 Door open alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.3.4 Alarm duration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
7.3.5 Alarm arming delay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.4 Chamber alarm system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
7.4.1 Temperature sensor system alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.4.2 Over-temperature cut-out and alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.5 CO
Auto-zero system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
2
7.5.1 Auto-zero . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
7.5.2 Changing auto-zero frequency . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
7.5.3 High temperature disinfection operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1 Routine maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.2 Daily Checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.3 Weekly checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.4 Monthly checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
8.1.5 CO
Sampling with analyzer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
2
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
8.1.6 Routine checks of the O2 control option (170 R only). . . . . . . . . . . . . . . . . . . . . . . . . . 52
8.2 Service. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
8.2.1 Fuse replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
8.3 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
8.4 Disinfection/Decontamination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
8.5 High temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
9.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
9.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
9.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
10 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.1 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.1.1 Equipment dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.1.2 Internal dimensions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.1.3 Transporting dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.1.4 Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.2 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.2.1 Mains/electrical supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.3 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.4 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.4.1 Temperature management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
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10.4.2 CO
control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
2
10.4.3 Relative humidity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
10.4.4 Altitude limit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
10.4.5 Storage temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
10.4.6 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
5
11 Ordering information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
11.1 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
11.2 Available options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
12 Equipment options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
12.1 Cooling system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
12.1.1 Troubleshooting the cooling system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
12.2 BMS relay contact alarm. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
12.3 High temperature disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
12.3.1 Using the high temperature disinfection (Galaxy R). . . . . . . . . . . . . . . . . . . . . . . . . . . 68
12.3.2 Using the high temperature disinfection (Galaxy S) . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
12.3.3 High temperature disinfection option with oxygen control . . . . . . . . . . . . . . . . . . . . . 71
12.4 O
control (1 - 19 %) (Galaxy R only). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
2
12.4.1 Setting up the N
tank. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
2
12.4.2 Setting up oxygen control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
12.4.3 Operating guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
12.4.4 Referencing to atmosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
12.4.5 Replace sensor soon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
12.4.6 Replace sensor now . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
12.4.7 Removing and replacing O
sensor. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
2
12.4.8 Replacing the filter disc . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Table of contents
Galaxy
6
English (EN)
®
170 R/170 S CO2 Incubators
12.4.9 Troubleshooting the O2 sensor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
12.4.10 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
12.5 O
control (0.1 - 19 %) (Galaxy R only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
2
12.5.1 Setting up the N
12.5.2 Setting up O
12.5.3 Enabling or disabling O
tank. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
2
control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80
2
control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
2
12.5.4 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
12.5.5 Referencing to atmosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
12.5.6 Programming desired O
level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
2
12.5.7 Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
12.6 O
control (1 - 95 %) (Galaxy R only). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
2
12.6.1 Setting up the N
12.6.2 Setting up O
12.6.3 Enabling or disabling O
12.6.4 Important notes for O
tank. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
2
control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
2
control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
2
levels > 80 % . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
2
12.6.5 Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
12.6.6 Referencing to atmosphere . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
12.6.7 Programming desired O
level . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
2
12.6.8 Other precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
12.6.9 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
12.7 O
Sensor replacement (Galaxy R only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
2
12.8 IP66 sealed electrical outlet socket. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
12.9 Humidity alert and monitoring package (Galaxy R only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.9.1 Humidity tray warning system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
12.9.2 Humidity display and alarm system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
12.10 Copper inner chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
13 Stacking stand installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
13.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
13.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
13.3 General description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
13.3.1 Ordering options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
13.3.2 Stacking frame kit contents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
13.4 Instructions for stacking incubators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
13.5 Instructions for stacking a different model CO
incubator onto a Galaxy 170 . . . . . . . . . . . . . 102
2
14 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
14.1 Declaration of conformity for 170 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
14.2 Declaration of conformity for 170 S . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Operating instructions
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
1 Operating instructions
1.1 Using this manual
Carefully read this operating manual before using the device for the first time.
Also observe the operating manual enclosed with the accessories.
The operating manual should be considered as part of the product and stored in a location that is easily
accessible.
When passing the device on to third parties, be sure to include this operating manual.
If this manual is lost, please request another one. The current version can be found on our website
www.eppendorf.com.
1.2 Danger symbols and danger levels
1.2.1 Hazard symbols
7
Hazard point Burns
Electric shock Material damage
Explosion Heavy loads
Inhalation Crush
1.2.2 Degrees of danger
The following degree levels are used in safety messages throughout this manual. Acquaint yourself with
each item and the potential risk if you disregard the safety message.
DANGER Will lead to severe injuries or death.
WARNING May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
Operating instructions
®
8
Galaxy
English (EN)
170 R/170 S CO2 Incubators
1.3 Symbols used
Example Meaning
You are requested to perform an action.
1.
2.
• List.
Perform these actions in the sequence described.
References useful information.
2 Product description
2.1 Main illustration
2.1.1 Galaxy 170 R/170 S CO2 Incubators
Abb. 2-1: Front and rear view of the 170 R/170 S CO2 Incubators
Galaxy
®
170 R/170 S CO2 Incubators
English (EN)
Product description
9
21
3 65
4
Fig. 2-1: Front and rear view of the 170 R/170 S CO2 Incubators
1 Sensor cover holder (present on older models)
2CO
sample port
2
3 Display/Interface (170 R display shown)
4 Inline regulator location (recommended)
5 Access port
6 Heat exchanger (cooling option only)
7 Left-hand side of control box
8 Right-hand side of control box
78
10
Product description
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
Abb. 2-2: Left-hand side control box
2
1
3
Fig. 2-2: Left-hand side control box
1 BMS relay contact alarm socket (Optional) 2 Auto-Zero filter
Abb. 2-3: Right-hand side control box
®
Galaxy
170 R/170 S CO2 Incubators
11
English (EN)
1
2
7
Product description
6
5
4
Fig. 2-3: Right-hand side control box
1 On/Off switch
2 Mains/power cord receptacle
3 Label
4 Fuse holder
3
inlet (170 R optional only, option not
5N
2
available on 170 S)
6CO
7O
inlet
2
inlet (170 R optional only, option not
2
available on 170 S)
12
Product description
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
2.2 Delivery package
Quantity Description
1 Operating manual
4
2
1
1
2
1
1
1
4
2
1
3
3
2
Non-tip perforated shelves
Installed
Wired shelf racks
Installed
Humidity tray
Installed
White porous CO
Installed
Packed in accessories bag
Black sensor cover
Installed
Mains/power cord
Packed in box inside outer carton
6 mm (~1/4 in) bore PVC hose, with large white filter
Packed in accessories bag
Screw-in lifting handles
Packed in accessories bag
Tubing clips
Packed in accessories bag
Auto-zero CO
inlet filter
2
Installed
Spare shelf rack foot
Packed in accessories bag
Spare shelf rack spacer
Packed in accessories bag
Silicone rubber suction feet
Packed in accessories bag (4 for 170 S)
sensor cover
2
2.2.1 Inspection of boxes
Inspect the boxes carefully for any damage that may have occurred during shipping. Report any damage to
the carrier and to your local Eppendorf sales order department immediately.
Product description
English (EN)
Galaxy
®
170 R/170 S CO2 Incubators
2.2.2 Packing list verification
Unpack your order, saving the packing materials for possible future use. Save the operating manual for
instruction and reference. Verify against your Eppendorf packing list that you have received the correct
materials, and that nothing is missing. If any part of your order was damaged during shipping, is missing,
or fails to operate, fill out the "Customer Feedback" form, available online at
newbrunswick.eppendorf.com/.
http://
2.3 Features
The Galaxy 170 R/170 S CO2 Incubator is microprocessor-controlled and designed to ensure accurate and
reliable operation.
2.3.1 Control system
13
The incubator incorporates a sophisticated control system that allows for easy programming, control and
monitoring of the chamber conditions.
2.3.2 Direct heating system
A direct heating system, utilizing a thermal heating element, completely surrounds the incubator, providing
an even temperature within the chamber. The independently and directly heated outer door is designed to
ensure an even distribution of heat. This system ensures a rapid, controlled return to optimum chamber
conditions after a door opening while also preventing any overshoot. The incubator’s direct heat system
provides for optimal use of laboratory space by allowing the most efficient internal volume for the footprint
of the instrument.
2.3.3 Infrared sensor
A solid-state infrared sensor is used to control the level of CO2, providing excellent reliability, while
remaining unaffected by humidity. The CO2 system has a programmable automatic zero system (Auto-zero)
to re-reference the sensor baseline to atmospheric CO
filtered atmospheric gas to the sensor. The chamber atmosphere within the sensor is completely displaced,
allowing the control system to automatically reference the sensor, after which the pump is switched off,
allowing the chamber atmosphere to homogenize back into the sensor. This provides for accurate CO
control without disturbing the chamber environment. For programming information (see Temperature and
CO
level on p. 32). For Auto-Zero instructions (see PROGRAMMABLE CO2 AUTOZERO on p. 34).
2
levels at regular intervals. A small pump supplies
2
2
2.3.4 Controlled humidity tray
A controlled water tray at the bottom of the incubator allows a high, uniform relative humidity (RH) while
preventing condensation in other parts of the chamber. Perforated shelves are provided as standard to
facilitate a much faster recovery of RH conditions in the chamber than with unperforated shelves.
14
Product description
®
Galaxy
English (EN)
170 R/170 S CO2 Incubators
2.3.5 Seamless chamber
The 170-liter chamber is seamless, to provide a sanitary and easy-to-clean environment, and all internal
components are manufactured from polished stainless steel. The shelves (which are non-tip), shelf racks
and humidity tray are easily removed without tools for thorough cleaning and are capable of being
sterilized. Air circulation is achieved without the use of a fan, eliminating duct work (a potential source of
contamination), simplifying cleaning, eliminating vibration, and facilitating use of microplates and
low-volume culture.
2.3.6 Standard features
The Galaxy 170 R/170 S contain many standard features usually seen as options. It has a sealed inner glass
door with a cam action lock to allow viewing of the cultures without compromising the internal atmosphere.
This is also available as a split 4- or 8-inner-door option (to coordinate with shelves), which is ideal for
critical hypoxic studies. In addition, there is a 25 mm (1 in) access port now standard to allow for seamless
integration of independent probes or other equipment through the chamber.
2.3.7 Multiple options
The incubator features multiple options that can be installed to simplify maintenance and provide superior
control over experimental conditions. For example, high-temperature disinfection quickly and conveniently
disinfects the incubator’s chamber at 120 °C, without the need to remove interior components or the CO
sensor. A humidity alert and monitoring package display relative humidity levels in the chamber on the
display and warns the user before the humidity tray runs out of water, preventing dehydration of samples.
Oxygen control provides for conditions that require above- or below-ambient oxygen levels. These and
other options and accessories provide for a uniquely flexible CO
demanding requirements. For details on equipment options (see Equipment options on p. 65).
incubator capable of meeting the most
2
2
2.3.8 Two-level alarm system
The incubator incorporates a two-level alarm system. The system alarms occur only if a problem develops
with system components that require user intervention to rectify. The incubator also incorporates an
over-temperature safety system that operates independently from the main control system.
2.4 Stacking devices
The incubator is designed so that one incubator can be safely stacked on top of either another Galaxy 170 R
or a Galaxy 170 S using the optional stacking kit, which includes instructions. It is not possible to put any
other type of incubator or heavy apparatus on top, as the top cover and stacking kit are designed to support
only the feet of another Galaxy 170 incubator. For installation instructions (see Stacking stand installation on
p. 93).
Product description
English (EN)
Galaxy
®
170 R/170 S CO2 Incubators
2.5 Optional equipment
The equipment options are factory-installed and model-dependent. A precise description of the equipment
options can be found in this manual (see Equipment options on p. 65).
15
16
Product description
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
Safety
English (EN)
Galaxy
®
170 R/170 S CO2 Incubators
3 Safety
3.1 Intended use
Eppendorf line of CO2 Incubators are microprocessor-controlled instruments designed for cell culture. The
direct-heated, fanless chambers are designed to provide high humidity levels, minimal vibration and
precisely-regulated atmosphere of temperature and gas(ses) required for cell growth in T-flasks,
microplates, and other cultureware. They are intended for indoor laboratory use, only.
CAUTION! Lack of safety due to incorrect accessories or spare parts
Accessories and spare parts that are not recommended by Eppendorf compromise the safety,
function and precision of the device. Eppendorf cannot be held liable or accept any liability for
damage resulting from the use of non-recommended accessories and spare parts.
Only use accessories and original spare parts recommended by Eppendorf.
17
3.2 User profile
The device may only be operated by trained lab personnel who have carefully read this operating manual
and are familiar with the device functions.
3.3 Application limits
3.3.1 Description of ATEX Guideline (94/9EC)
DANGER! Explosion hazard
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which could create an explosive
atmosphere.
Due to its design and the ambient conditions in its interior, the device is not suitable for use in potentially
explosive atmospheres.
The device may only be used in a safe environment, e.g., the open atmosphere of a ventilated lab.
The use of substances which may contribute to a potentially explosive atmosphere is not permitted.
The final decision regarding the risks associated with using these types of substances is the user's
responsibility.
18
Safety
®
Galaxy
English (EN)
170 R/170 S CO2 Incubators
3.4 Information on product liability
In the following cases, the designated protection of the device may be compromised.
The liability for the function of the device passes to the operator if:
• The device is not used in accordance with this operating manual.
• The device is used outside of the range of application described in the succeding chapters.
• The device is used with accessories or consumables that were not approved by Eppendorf.
• Service or maintenance is completed on the device by people who are not authorized by Eppendorf.
• The owner has made unauthorized modifications to the device.
3.5 Warnings for intended use
Before using the device, read the operating manual and observe the following general safety instructions.
3.5.1 Personal injury and damage to device
WARNING! Risk of personal injury
Elevated levels of CO
incubator.
Wear personal protective equipment (PPE).
WARNING! Risk of personal injury
Burns due to hot surface.
Do not touch the equipment during the high temperature disinfection cycle.
Do not open equipment door during the cycle.
CAUTION! Risk of personal injury
At least four people are required to safely lift the incubator.
NOTICE! Risk of material damage
Never try to lift the incubator by its door; this would cause permanent damage to the
incubator.
may be found in and around the operating area of the CO2
2
NOTICE! Risk of material damage
To avoid possible damage to the CO
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
sensor, never leave water in the humidity tray while
2
Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor (if installed) removal.
NOTICE! Risk of material damage
Galaxy
®
170 R/170 S CO2 Incubators
English (EN)
Safety
19
CO
gas pressure must not exceed 5 PSI (0.35 bar).
2
NOTICE! Risk of material damage
Working with electrical power inside a humid environment (where the incubator is
humidified) can cause damage. The following precautions should be observed:
The instrument or equipment, and its external connections, to be used inside the chamber
should be specified as suitable for use in a humid environment, and at 37 °C (see also
“Using Powered Equipment within the Chamber”). If in doubt, consult with the
manufacturer of the equipment.
Always ensure the connections are properly and securely made.
Be sure to switch OFF the green illuminated switch on the front left of the incubator before
connecting or disconnecting equipment inside the chamber, if equipped with optional IP66
socket.
The Sealing Cap must always be in place when the socket is not in use.
Both the incubator and the IP66 enclosure must be plugged into an electrical supply
protected by an RCD device. Any device chosen must be a self-resetting type which will
automatically reconnect power to the incubator as soon as power is restored following a
power failure.
20
Safety
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
Installation
English (EN)
Galaxy
®
170 R/170 S CO2 Incubators
4 Installation
4.1 Utilities requirements
The following utilities requirements are needed for operation:
Utility Requirement
Electricity 120 V, 50/60 Hz earthed/grounded mains/electrical supply with minimum
capacity of 10 amps
230 V, 50/60 Hz earthed/grounded mains/electrical supply with minimum
capacity of 8 amps
CO2 gas Cylinder with 100 % CO2 vapor withdrawal, together with a two-stage
regulator for pressure control to 18.85 PSI (1.3 bar)
CO
gas pressure must not exceed 5 PSI (0.35 bar). It is recommended that a in-line pressure
2
regulator be used for each gas type introduced into the incubator to precisely control gas
pressure (see Accessories on p. 63).
21
4.2 Selecting the location
Select a level surface capable of withstanding the operating weight of the incubator. Actual operating
weight will be dependent on both the options installed, and the material stored in the incubator.
The incubator is designed to operate at a chamber temperature of 4.0 °C above ambient, and at an absolute
minimum ambient temperature of 15 °C if the incubator is being operated at 37 °C. Maximum allowable
ambient temperature is 28 °C.
Position incubator to allow clearance for opening door and access to the CO
located on the left side of the incubator.
Avoid placing the incubator in areas that may affect performance, such as those listed below.
DO NOT place the incubator:
• Directly under, beside or within the air flow of heating or air-conditioning ducts, or other drafts;
• Directly beside heat-generating equipment such as a heater, an autoclave or an oven;
• Near the exhaust of heat- or cold-generating equipment;
• Near a window exposed to direct sunlight;
• Directly on top of any heat-generating apparatus;
• Without minimum ventilation clearance of 10 mm (0.5 in) all around (50 mm (2 in) in back if you have
the cooling system option).
sample port
2
22
Installation
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
4.3 Unpacking the incubator
CAUTION! Risk of personal injury
At least four people are required to safely lift the incubator.
NOTICE! Risk of material damage
Never try to lift the incubator by its door; this would cause permanent damage to the
incubator.
1. Install the four lifting handles into the tapped holes on both sides of the incubator.
Abb. 4-1: Installing lifting handles
1
Fig. 4-1: Installing lifting handles
1 Access port 2 Lifting handles
2. Carefully move the incubator to its operational location using lifting handles.
Silicone rubber suction feet are supplied for non-slip application.
3. Remove all internal packaging.
4. Remove the four lifting handles from the incubator and store for future use.
4.4 Initial setup
2
1. Place the silicone rubber suction feet onto the incubator’s adjustable feet.
Keep silicone rubber suction feet installed at all times.
2. Place two rubber shelf rack feet, and one shelf rack spacer onto each shelf rack (see Fig. 4-2 on p. 23).
3. Place the two shelf racks inside the chamber. Ensure that the cushioned tubing spacers are snug against
the side walls; these spacers allow clearance for the shelves.
Abb. 4-2: Inserting shelf racks
Galaxy
®
170 R/170 S CO2 Incubators
English (EN)
Installation
23
112
Fig. 4-2: Inserting shelf racks
1 Shelf rack feet 2 Shelf rack spacer
Only shelf racks for four shelves are shown in this manual. Shelf racks for eight shelves are
also available.
4. Install the tie rod at the back of the shelves to hold both sides together.
24
Installation
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
Abb. 4-3: Installing tie bar to shelf racks
1
2
Fig. 4-3: Installing tie bar to shelf racks
1 Tie rod 2 Tie rod anti-tip groove
5. Install the shelves, top to bottom. Ensure that each shelf’s anti-tip groove is properly inserted (faced
upward and to the rear of the incubator) onto each of the shelf rack guides.
Abb. 4-4: Inserting shelves
Fig. 4-4: Inserting shelves
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
6. Level the incubator by adjusting the feet. Place a small level on the second shelf of the incubator. Adjust
the leveling feet until the incubator is level and stable. Lock the leveling feet in place by tightening the
locking nuts on each foot.
7. Slide the humidity tray onto the lowest shelf rack support.
Abb. 4-5: Installing the humidity tray
Installation
25
Fig. 4-5: Installing the humidity tray
Abb. 4-6: Humidity tray installed
Fig. 4-6: Humidity tray installed
26
Installation
®
Galaxy
English (EN)
170 R/170 S CO2 Incubators
4.5 Making connections
WARNING! Risk of personal injury
Elevated levels of CO
incubator.
may be found in and around the operating area of the CO2
2
Wear personal protective equipment (PPE).
WARNING! Danger due to incorrect power supply
Only connect the device to voltage sources that meet the requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power cable.
1. Remove sensor (CO2, O2, and RH) protective cover (option dependant), and store for future use.
2. Remove gas inlet connector protection cap.
®
Galaxy
170 R/170 S CO2 Incubators
3. Attach supplied 6 mm bore PVC hose with large white filter to gas inlet connector.
Abb. 4-7: Making connections
1
2
7
Installation
27
English (EN)
Fig. 4-7: Making connections
1 On/Off switch
2 Mains/power cord receptacle
3 Label
4 Fuse holder
6
3
5
4
inlet (170 R optional only, option not
5N
2
available on 170 S)
6CO
7O
inlet
2
inlet (170 R optional only, option not
2
available on 170 S)
4. Attach recommended optional in-line pressure regulator (not supplied), if applicable. For connection
location (see Fig. 2-1 on p. 9).
28
Installation
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
Attaching in-line pressure regulator, (not supplied), is a recommended option.
Abb. 4-8: In-line pressure regulator
Fig. 4-8: In-line pressure regulator
• The in-line pressure regulator controls Secondary Gas Pressure. A default pressure setting
of 5 PSI (0.35 bar) is recommended.
• A large size cylinder of “CO
- Vapour Withdrawal” is required to supply the incubator.
2
This cylinder controls Primary Gas Pressure. Fitting a Two-Stage CO
is recommended.
• Ensure the protective cover(s) are removed from all sensor(s) and replaced in the holder
for safekeeping. Be very careful, as you remove the black CO
accidentally remove the white porous sensor cover. This must remain in place.
5. Secure tubing using the supplied hose clips.
Pressure Regulator
2
sensor cover, not to
2
The inlet filter may already be installed on the equipment tray in a filter housing, if supplied
loose (packed in accessories bag) the part needs to be installed before use.
6. Attach CO
inlet filter to Auto-Zero connection, located on side of the equipment box.
2
7. Confirm voltage requirements.
Cross reference incubator label information on side of equipment box.
8. Using mains/power cord supplied, connect incubator to correct mains/voltage supply.
9. Turn incubator On.
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
5 Operation
5.1 Preparing for operation
1. Remove the black protective cover from the CO2 sensor, taking care not to remove the white porous
cover; store the black cover on the sensor cover holder on the side of the incubator. The sensor cap
should be placed back on the sensor when the incubator is to be cleaned.
Abb. 5-1: CO2 Incubator rear view
1
2
3
Operation
29
6
5
4
Fig. 5-1: CO2 Incubator rear view
1 Access port
2CO
sample port
2
3 Sensor cover holder
4 Removable lifting handles
5 Location for mounting in-line regulators
sensor location (not shown here)
6O
2
2. Ensure that the white porous sensor cover remains in place.
3. Using the mains/power cord provided, connect the incubator to a earthed/grounded mains/power
supply.
4. Switch the incubator ON using the on/off switch at the rear of the cabinet.
The display will illuminate immediately.
5. Turn on the CO
6. The chamber setpoints are pre-programmed at 37.0 °C and 5 % CO
gas supply with the pressure regulator set to 5 PSI (0.35 bar).
2
. Leave the incubator on until the
2
programmed chamber temperature and CO2 concentration have been reached.
30
Operation
®
Galaxy
170 R/170 S CO2 Incubators
English (EN)
• The incubator’s CO
After the temperature setpoint is reached, the CO
valve is disabled until the incubator reaches the temperature setpoint.
2
valve is activated, allowing the
2
incubator to reach the CO2 setpoint.
• If power is interrupted to the incubator long enough for the temperature to drop below
setpoint, the CO
(This serves to avoid spurious CO
valve will be deactivated until temperature setpoint is again achieved.
2
readings while the incubator is below its temperature
2
setpoint)
7. Leave the incubator running for at least two hours (preferably overnight) to allow conditions to stabilize.
5.2 Using the humidity tray
NOTICE! Risk of material damage
To avoid possible damage to the CO
the incubator is switched off, or when a high temperature disinfection cycle is initiated
(optional feature).
Allow a clearance of 50 mm (2 in) to allow access for oxygen sensor (if installed) removal.
• The humidity tray should be left in place at all times.
• Use distilled water only in the humidity tray. Use of any other types of water including
deionized water will cause corrosion inside the incubator.
sensor, never leave water in the humidity tray while
2
If humidification is required:
1. Fill the humidity tray with 1.5 - 2.5 liters of warm (37.0 °C) distilled water.
2. For cell culture work, we recommend adding copper sulphate in the humidity tray. Tests have shown
that, in addition to inhibiting bacterial growth in the tray, this can reduce contamination on the chamber
walls. Add one small teaspoonful (~0.5 g) of copper sulphate to the water in the humidity tray.
3. For sensitive work, we do not recommend the use of any biocide in the humidity tray. To reduce the
possibility of contamination, every 10 to 14 days, empty the tray, clean it with a solution of 70 %
isopropyl alcohol and 30 % distilled water, and then refill it with 1.5 liters of warm distilled water.
The humidity level within the chamber is not adjustable. The internal chamber will reach ~95
% relative humidity at 37 °C using the 1.5-liter humidity tray.
5.3 Operation for optional features
For functionality on optional features (see Available options on p. 64).
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