BUCHI C-640 User Manual

Operation Manual

UV-Vis Detector

C�640

11593492

en

Table of contents

Table of contents

1 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.1 User qualification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.2 Proper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.3 Improper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.4 Safety warnings and safety signals used in this manual . . . . . . . . . . . . . . . . . 7

2.5 Product safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.5.1 General hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

2.5.2 Safety measures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2.5.3 Built-in safety elements and measures . . . . . . . . . . . . . . . . . . . . . . . . 10

2.6 General safety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1 Scope of application and delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1.1 Standard instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.1.2 Standard equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.2 Optional accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.3 Recommended spare parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

3.4 Technical specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

3.5 Configuration of the analog cinch output . . . . . . . . . . . . . . . . . . . . . . . 15

3.6 Light sources . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.6.1 Deuterium lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.6.2 Halogen lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

3.7 Signals and parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.1 Light intensity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.2 Range of wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.3 Optical and electronical half width – spectral resolution . . . . . . . . . . . . . . . . 16

3.7.4 Time constant / data filtering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.5 Analog range ratio. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.6 Display brightness. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.7 Subscription / Remote or local control. . . . . . . . . . . . . . . . . . . . . . . . . 16

3.7.8 Scanning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

4 Description of function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1 Front panel of the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

4.1.1 LED status . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18

Read this manual carefully before installing and running your system and note the safety precautions
in chapter 2 in particular. Store the manual in the immediate vicinity of the instrument, so that it can be
consulted at any time.
No technical modifications may be made to the instrument without the prior written agreement of
BUCHI. Unauthorized modifications may affect the system safety or result in accidents.
This manual is copyright. Information from it may not be reproduced, distributed, or used for competi­tive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.

If you need another language version of this manual, you can download available versions
at www.buchi.com.

3 C-640 Operation Manual, Version A

Table of contents

4.1.2 Icons on the graphical display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.1.3 Push-buttons of the front panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.2 Connections at the rear panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

4.3 The cell compartment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

4.3.1 Removing the cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

4.3.2 Mounting the cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

5 Putting into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.1 Installation site. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23

5.2 Installing the Detector C-640 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.2.1 Electrical connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.2.2 Connecting the communication cable . . . . . . . . . . . . . . . . . . . . . . . . . 24

5.2.3 Connecting the tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

5.3 Starting the detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

5.4 Controlling the detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

5.4.1 The start up screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.4.2 Switching the lamp on / off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.4.3 Measuring / Zeroing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.5 Parameter setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.5.1 Wavelength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

5.5.2 Time constant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.5.3 Half width . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.5.4 Analog range ratio. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.5.5 Display brightness. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

5.6 Getting information and scanning . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.6.1 General information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.6.2 Intensity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.6.3 Calibration menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

5.6.4 Errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

6 Maintenance and repairs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

6.1 Customer service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

6.2 Housing condition . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32

6.3 Cleaning and replacement of the cell windows . . . . . . . . . . . . . . . . . . . . 32

6.3.1 Cleaning the cell window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.3.2 Replacing the cell windows . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

6.4 Replacement of the deuterium lamp. . . . . . . . . . . . . . . . . . . . . . . . . . 35

7 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

7.1 Internal display error codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

7.2 Hardware error codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

8 Shutdown, storage, transport and disposal . . . . . . . . . . . . . . . . . . . . . . . . 39

8.1 Storage and transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

8.2 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

9 Declarations and requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

9.1 FCC requirements (for USA and Canada) . . . . . . . . . . . . . . . . . . . . . . . 40

9.2 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

4 C-640 Operation Manual, Version A

1 About this manual

This manual describes the UV-VIS Detector C-640. It provides all information required for its safe
operation and to maintain it in good working order. It is addressed to laboratory personnel in particular.

Abbreviations

FEP

Fluorelastomer

PTFE Polytetrafluoroethylene (Teflon)

1 About this manual

5 C-640 Operation Manual, Version A

2 Safety

This chapter points out the safety concept of the instrument and contains general rules of behavior
and warnings from direct and indirect hazards concerning the use of the product.
For the users safety all safety instructions and the safety messages in the individual chapters shall
strictly be observed and followed. Therefore, the manual must always be available to all persons
performing the tasks described herein.

2.1 User qualification

The instrument may only be used by laboratory personnel and other persons, who on account of
training and professional experience know the dangers, which can develop when operating the
instrument.

Untrained personnel or persons, who are currently being trained, require careful supervision by a
qualified person. The present Operation Manual serves as a basis for training.

2 Safety

2.2 Proper use

The UV-VIS Detector C-640 has been designed and built as laboratory instrument.

It is able to detect and measure changes in the light absorption behavior of liquids passing through the
flow cell of the instrument. The absorption for four selected wavelengths is measured simultaneously
and scanning over the entire wavelength range of the detector (200 – 840 nm) becomes possible in
conjunction with the BUCHI SepacoreControl software and the control unit C-620.

A deuterium lamp acts as primary light source of the instrument, supported by a halogen lamp as
secondary light source. The communication between the C-620 control unit and the SepacoreControl
software is done via a serial RS232 cable (contained in the standard equipment). If the C-640 detector
shall be used without the C-620 control unit, it can be connected directly to the C-660 fraction
collector using the optional available cinch cable.

If the instrument is used with potentially toxic or hazardous substances, it has to be installed inside a
closed fume hood or glove box. In such case, the complete processing and system handling has to
be performed within the ventilated box to avoid toxication and other hazardous situations to the user
and the environment.

2.3 Improper use

Applications not mentioned in section 2.2 are considered to be improper. Applications which do not
comply with the technical data (see section 3 of this manual) are also considered to be improper.

The operator bears the sole risk for any damages or hazards caused by improper use!

The following uses are expressly forbidden:

• Installation or use of the instrument in rooms, which require ex-protected instruments.

6 C-640 Operation Manual, Version A

2.4 Safety warnings and safety signals used in this manual

DANGER, WARNING, CAUTION and NOTICE are standardized signal words for identifying risk levels,
related to personal injury and property damage. All signal words, which are related to personal injury
are accompanied by the general safety sign.

For your safety it is important to read and fully understand the below table with the different signal
words and their definitions!

Sign Signal word Definition Risk level

DANGER

Indicates a hazardous situation which, if not avoided, will result in
death or serious injury.

2 Safety

★★★★

WARNING

CAUTION

NOTICE

no

Supplementary safety information symbols may be placed in a rectangular panel on the left to the
signal word and the supplementary text (see below example).

Space for

supplementary

safety

information

symbols

Table of supplementary safety information symbols
The below reference list incorporates all safety information symbols used in this manual and their

meaning.

Indicates a hazardous situation which, if not avoided, could result
in death or serious injury.

Indicates a hazardous situation which, if not avoided, may result
in minor or moderate injury.

Indicates possible property damage, but no
practices related to personal injury.

!

SIGNAL WORD

Supplementary text, describing the kind and level of hazard / risk seriousness.

• List of measures to avoid the herein described, hazard or hazardous situation.

• ...

• ...

(property damage only)

★★★☆

★★☆☆

★☆☆☆

Symbol Meaning

General warning

Electrical hazard

7 C-640 Operation Manual, Version A

Explosive gases, explosive environment

Harmful to live-forms

Device damage

Wear laboratory coat

2 Safety

Wear protective goggles

Wear protective gloves

Additional user information
Paragraphs starting with NOTE transport helpful information for working with the device / software or

its supplementaries. NOTEs are not related to any kind of hazard or damage (see example below).

NOTE

Useful tips for the easy operation of the instrument / software.

8 C-640 Operation Manual, Version A

2.5 Product safety

Safety warnings in this manual (as described in section 2.4) serve to make the user alert and to avoid
hazardous situations emanating from residual dangers by giving appropriate counter measures.
However, risks to users, property and the environment can arise when the instrument is damaged,
used carelessly or improperly.

2.5.1 General hazards

The following safety messages show hazards of general kind which may occur when handling the
instrument. The user shall observe all listed counter measures in order to achieve and maintain the
lowest possible level of hazard.

Additional warning messages can be found whenever actions and situations described in this manual
are related to situational hazards.

2 Safety

!

Warning

Death or serious injuries by use in explosive environments.

• Do not operate the instrument in explosive environments.

• Do not operate the instrument with explosive gas mixtures.

!

Warning

Death or serious poisoning by contact or incorporation of harmful substances at use.

!

Warning

Death or serious injuries by contact with high voltage.

• Only open the housing of the product when instrument is switched off and unplugged.

Risk of instrument short-circuits and damage by liquids.

• Do not spill liquids over the instrument or parts of it.

• Wipe off any liquids instantly.

• Do not move the instrument when it is in use.

• Keep external vibrations away from the instrument.

9 C-640 Operation Manual, Version A

Notice

2 Safety

Risk of instrument damage by wrong mains supply.

• External mains supply must meet the voltage given on the type plate.

• Check for sufficient grounding.

2.5.2 Safety measures

Always wear personal protective equipment such as protective eye goggles, protective clothing, and
gloves when working with the instrument.

2.5.3 Built-in safety elements and measures

High voltage and electrostatic charges

• Safety current limitation.

• Grounded housing to arrest electrostatic charges.

Notice

2.6 General safety rules

Responsibility of the operator
The head of laboratory is responsible for training his personnel.

The operator shall inform the manufacturer without delay of any safety-related incidents which might
occur during operation of the instrument. Legal regulations, such as local, state and federal laws
applying to the instrument must be strictly followed.

Duty of maintenance and care
The operator is responsible for the proper condition of instrument at use and that maintenance,

service and repair jobs are performed with care and on schedule by authorized personnel only.

Spare parts to be used
Use only genuine consumables and genuine spare parts for maintenance to assure good system

performance and reliability. Any modifications to the spare parts used are only allowed with the prior
written permission of the manufacturer.

Modifications
Modifications to the instrument are only permitted after prior consultation with and with the written

approval of the manufacturer. Modifications and upgrades shall only be carried out by an authorized
BUCHI technical engineer. The manufacturer will decline any claim resulting from unauthorized modifi­cations.

10 C-640 Operation Manual, Version A

3 Technical data

This chapter introduces the reader to the instrument and its specifications. It contains the scope of
delivery, technical data, requirements and performance data.

3.1 Scope of application and delivery

The UV-VIS Detector C-640 is available as powerful detector for the Sepacore system. It can be used
in conjunction with the SepacoreControl software, connected to the C-620 control unit or without the
use of the software, connected to the C-660 fraction collector.

The instrument can measure the change of the absorption behavior of a sample at four selected
wavelengths in the range of 200 to 840 nm.

In conjunction with the SepacoreControl software it is also able to scan the whole range of the spec­trum continuously.
The scope and completeness of delivery can be checked according to section 3.1.2 Standard equip­ment. After unpacking please check the instrument and all accessories thoroughly for any damages!

3 Technical data

NOTE

For additional information about the listed products, see www.buchi.com or contact your local dealer.

3.1.1 Standard instrument

Table 3-1: Standard instrument

Product Order no.

UV-VIS Detector C-640
with preparative flow cell

Complete UV-VIS Detector C-640 system for use
in conjunction with the SepacoreControl software.
Complete with preparative flow cell, necessary cables
fittings and tubing.

11058148

11 C-640 Operation Manual, Version A

3.1.2 Standard equipment

Each part listed with an order number can be ordered separately as spare part.

3 Technical data

Table 3-2: Standard equipment

Product Order no.

UV-VIS detector unit C-640

Preparative flow cell for C-640 11057949

Fitting and ferrule 1/8” (2 pcs. each) 044261

Fitting and ferrule 1/16” (2 pcs. each) 044262

12 C-640 Operation Manual, Version A

3 Technical data

FEP tube inner diameter 1/16”, outer
diameter 1/8”

FEP tube inner diameter 1/32”, outer
diameter 1/16”

Test cell for preparative cell

CD containing Operating Instructions

en / de / fr / it / es / cn / jp

Power cord

044354

044357

3.2 Optional accessories

3.3 Recommended spare parts

RS232 cable

Table 3-3: Optional accessories

Product Order no.

Cinch cable 045216

For connecting the C-640 directly to the
C-660 fraction collector without using the
SepacoreControl software.

Table 3-4: Recommended spare parts

Product Order no.

Deuterium lamp for C-640 11058131

Spare part set preparative flow cell 11058231

13 C-640 Operation Manual, Version A

3.4 Technical specifications

Table 3-5: Technical specifications

Average power consumption max. 90 W

Power supply voltage 100–240 VAC ± 10%

Frequency

Fuse 2.5 A / 250 V
Dimensions (w × d × h) 135 × 418 × 185 mm

Weight 6 kg

Wavelength range 200–840 nm (256 elements on CCD)

Typical spectral half width 10 nm

Accuracy of adjustment ± 1 nm

Reproducibility ± 0.5 nm

Noise level at test cell ± 5 x 10

Drift at test cell (254 nm after 1 h) 1 x 10

Time constant (T90) 0.5 s ; 0.75 s; 1 s or 2 s

Light source Deuterium discharge lamp / halogen lamp

Maximum flow rate 500 mL/min

Maximum pressure at the cell 20 bar / 290 psi

Output for integrator 1 V/AU (in digital form only)

Interface RS232

Installation category II

Degree of protection IP20

Pollution degree 2

Environmental conditions:

Temperature 5–40 °C for indoor use only

Altitude up to 2000 m

Humidity

3 Technical data

50 / 60 Hz

-5

AU at 254 nm, TC 0.75 s

-3

AU/h

max. relative humidity 80% for temperatures up to
31 °C, linearly decreasing to 50% at 40 °C

Table 3-6: Material and Approvals

Materials in contact with mobile phase PTFE / fused silica / stainless steel

Approvals CE, CSA

14 C-640 Operation Manual, Version A