Ethidium Bromide Solution,
10 mg/ml
Catalog Number
161-0433
Table of Contents
Section 1 Introduction................................. 1
1.1 Specifications ...................................... 2
Section 2 Instructions for Use .................... 2
2.1 Gel Staining Instructions ..................... 3
2.2 CsC1 Gradient Instructions ................. 4
Section 3 Health Hazards ........................... 4
3.1 Risk and Safety Phrases....................... 5
Section 4 Ordering Information ................ 8
1
Section 1
Introduction
Bio-Rad’s ethidium bromide solution
increases safety in laboratory procedures by
eliminating the handling of powdered ethidium
bromide, a known carcinogenic reagent. A liquid
dropper is provided with the solution to
eliminate contamination of pipettes and pipette
tips. The liquid dropper dispenses approximately
50 µl per drop.
2
Wear gloves at all times and wash contaminated
skin with water. Refer to Section 3 of this manual
for additional Health Hazard information.
2.1 Gel Staining Instructions
Make a 0.5 µg/ml working solution accord-
ing to the volume required:
0.5 µg/ml (1 liter) 0.5 µg/ml (200 ml)
Add one drop (50 µl) Add 10 µl of 10 mg/ml
of 10 mg/ml ethidium ethidium bromide solubromide solution to tion to 200 ml ddi water
1000 ml ddi water
Soak the gel for 15 minutes with gentle
agitation. Longer staining times will result in
high background. Rinse the gel with ddi water
and destain with fresh ddi water for 15 minutes
with gentle agitation. At this point, the gel
should be ready for photographing, although
another 15 minute rinse with fresh ddi water will
reduce background further.
3
1.1 Specifications
Contents Ethidium bromide solution,
10 mg/ml, 10 ml
Application Agarose and polyacrylamide gel
staining
Large scale plasmid preparation
using cesium chloride gradients
Storage May be stored indefinitely in a
lightproof container at room temperature
Section 2
Instructions for Use
Ethidium bromide is used to visualize DNA
in agarose gels and in many cesium chloride gradient protocols. The 10 mg/ml ethidium bromide
stock solution can be diluted for gel staining or
used as provided for CsC1 gradient protocols.
Caution: Ethidium bromide is a toxic chemical
and a mutagen. It causes eye and skin irritation.
4
2.2 CsC1 Gradient Instructions
Use 10 mg/ml ethidium bromide solution as
provided. Add 0.8 ml of ethidium bromide
solution for every 10 ml of cesium chloride/DNA
solution to yield a final ethidium bromide
concentration of 740 µg/ml.
1
1. Maniatus, T., Fritsch, E.F. and Sambrook, J. Molecular
Cloning: A Laboratory Manual, Cold Spring Harbor
Laboratory (1989).
Section 3
Health Hazards
Ethidium bromide is a toxic chemical and a
mutagen. It causes eye and skin irritation. Wear
gloves at all times and wash contaminated skin
with water. Clean up liquid spills by absorbing
the liquid with absorbent material. Spray the area
with a 10% bleach solution, clean up with
absorbent material, rinse with water, and dry the
area with paper towels. Refer to the Risk and
Safety Phrases and the MSDS in this manual for
additional information.
3.1 Risk and Safety Phrases
Hazardous products are marked with
Hazard Symbols and Risk and Safety Phrases in
accordance with the rules of the European
Community Commission. Refer to the catalog
for a complete listing of Hazard Symbols and
Risk and Safety Phrases.
R 46
S 44, 53
EINECS 2149846
RTECS SF7950000
CASNr 1239-45-8
For laboratory use only.
5
6
May cause heritable genetic damage.
If you feel unwell, seek medical advice (show
the label where possible).
Avoid exposure - obtain special instructions
before use.
Kann vererbbare Schäden verursachen.
Bei Unwohlsein ärztlichen Rat Einholen. Wenn
möglich dieses Etikett vorzeigen.
Exposition vermeiden - vor Gebrauch besondere
besondere Anweisungen einholen.
Peut causer des altérations génétiques
héréditares.
En cas de malaise consulter un médecin (si possible lui montrer l’étiquette).
Exposition vermeiden - vor Gebrauch besondere
Anweisungen einholen.
Può provocare alterazioni genetiche ereditarie.
7
In caso di malessere consultare il medico (se possibile, mostrargli l’etichetta).
Evitare l’esposizione - procurarsi speciali
istruzioni prima dell’uso.
8
Section 4
Ordering Information
Catalog
Number Description
161-0433 Ethidium Bromide Solution, 10 mg/ml
161-0430 Ethidium Bromide Tablets, 10 x 10 mg
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules, CA 94547
4006020 Rev B
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