Bio-rad Econo System User Manual
Econo System
Instruction
Manual
For
Technical Service
Call Your Local Bio-Rad Office
in the U.S.
t=it•J;1
Calll-800-4BIORAD
( 1-800-424-6723)
9
•)
or
Warranty
Model
Serial Number
Date
Warranty Period
Unless otherwise specified, instruments sold by Bio-Rad Laboratories are
warranted for 1 year against defects in materials and workmanship.
If
replace the defective parts without charge. However, the following defects are
specifically excluded:
1.
Defects caused by improper operation.
2.
Repair or modification done by anyone other than Bio-Rad Laboratories
or an authorized agent.
3.
Use with tubings or fittings not specified by Bio-Rad Laboratories for use
with this system.
4. Deliberate
_______________
______________
of
Delivery
any defects should occur during this warranty period, Bio-Rad will
or
-------------
____________
accidental misuse.
_
_
_
5.
Damage caused by disaster.
6.
Damage due to use
7. Damage due to spills.
This warranty does not apply to tubing, fittings, and fuses.
For inquiry or request for repair service, contact Bio-Rad Laboratories after
confirming the model and serial number
For Technical
Call l-800-4BIORAD (1-800-424-6723)
of
improper solvents or samples.
of
your instrument.
Service Call Your Local Bio-Rad Office
or
in the U.S.
Table
of
Contents
Section 1
1.1
1.2
Section2
2.1
2.2
2.3
Section 3
3.1
3.2
3.3
Section 4
4.1
4.2
4.3
4.4
4.5
4.6
4.7
4.8
4.9
4.10
Introduction .................................................................. .
Overview ................................................................................. .
System Components ................................................................ .
Unpacking the Econo System Controller ................... .
Unpacking Instructions ........................................................... .
Voltage Considerations ............................................................ .
Safety Considerations .............................................................. .
1
1
2
3
3
4
5
Econo System Controller:
Physical Description and Control Features ............... .
Front Panel Functions .............................................................. .
Rear
Panel Functions ............................................................... .
Topside Functions .................................................................... .
6
6
7
8
Econo System Controller Connections and Set Up .... 9
Model EP-1 Econo Pump......................................................... 10
Model EM-1 Econo UV Monitor and Optics Module.............
Model
and Model
Model EG-1 Econo Gradient Monitor . . . .... ... . . ...
Model
Model
Solvent Reservoir.....................................................................
Model 1325 Econo (Single-pen) Recorder...............................
Model 1327 Econo (Dual-pen) Recorder.................................
Data Aquisition Systems ............................ .............................. 14
EV-1
2110 Fraction Collector
SV-3
Econo Buffer Selector
SV-5
Valve Pod......................................................
Diverter Valve......................................................
..
. . ...
...
. .......
..
. .
.. ..
...
. .
...
. . ... . .
.....
..
. . .... ...... ... 12
..
11
12
. . . . . 12
13
13
13
13
Section 5
5.1
5.2
5.3
5.4
5.5
5.6
5.7
5.8
Section 6
6.1
6.2
Plumbing the System..................................................... 14
Tubing and Fittings . .
Gradient Proportioning Valve and Mixer................................. 16
Model
Model
Manual Sample Injection Valve
and Chromatography Column . . . ... . .
UV Optics Module and Gradient Monitor Flow Cell . . . .
Model
Fraction Collector.....................................................................
EP-1 Econo Pump......................................................... 16
SV-5
Valve Pod.............................................................
SV-3
Diverter Valve ......................................................
..
... . . . . ..... ..... ... . .........
... . ..
.. . ..
. ........ ...........
. . ..... ... . .
.. . ..
..
.. . ..
. . .
..
. . ...
15
17
.. . .. . .. . 18
.. . .. ..
... 20
21
21
Getting Started .............................................................. 21
Connecting System to Power
System Check........................................................................... 22
..
. . . ..... ... . . ... ...... ....
..
..
. . .
..
......
...
..
...
21
Section 7
7.1
7.2
7.3
7.4
7.5
7.6
7.7
7.8
7.9
Programming the System .............................................
Getting Started:
Verification
Econo
!socratic (Single Buffer) Methods............................................
Binary Gradient Formation . ... . . . . .
Peak Detection and Collection .....
Programming Multi-Step Methods
(Using the Econo Buffer Selector)........................................... 35
Programming Multi-Step Methods Which Include a Binary
Gradient (Using the Econo Buffer Selector)............................ 38
Programming Automated
Options While Running A Method . . ..... ...
of
Econo System Software Version...................... 23
System Software Mode Selection................................. 24
..
. .
...
. .....
...
. . ...
..
... . ... . . . .... ...
.. . ...
......
..
..
......
...
..
... ....... ....
Sample Injection............................. 39
..
.... ... . . .... ... . . .
..
. . .
..
..
. .
...
...
..
23
25
28
31
40
Section 8
Section 9
Appendix A
Appendix B
Appendix C
Storage and Maintenance............................................. 42
Thoubleshooting ............................................................. 43
Interfacing and Signals .................................................
45
System Specifications .................................................... 47
Ordering Information................................................... 48
Section
1
Introduction
Fig. 1.1. The Automated Econo System.
1.1
Overview
The Automated Econo System consists
matography instruments which can be integrated to form a system. System
components include a complete line
cartridges, fittings, tubing, and valves. Each component has its own stand-alone
features in addition to its role in the system.
The Model ES-1 Econo System Controller integrates the individual com-
ponents into a low pressure chromatography system. The Econo System con-
troller provides the following functions:
• Gradient proportioning and mixing
• Peak detection by threshold
• Programmed control
Buffer Selector is used)
• Waste/collect diverter valve
• 6-port manual injector valve
of
up to 5 solutions (when the Model
of
or
time windows
of
a series
chromatography columns, prepacked
of
free-standing chro-
EV-1
Econo
• Solvent ice bath
These functions supplement those provided by the Model EP-1 Econo
Pump, Model EM-1
Monitor, Model EV-1 Econo Buffer Selector, and Model
Collector. This manual contains the necessary information for setting up and
Econo
UV
Monitor, Model EG-1 Econo Gradient
2110 Fraction
1
running the system. In some sections, references will be made to informati1
provided in the component manuals. A binder is provided to help organi
the information.
1.2 System Components
The complete Automated Econo System consists
ponents:
• Model EP-1 Econo Pump
• Model EM
• Model ES-1 Econo System Controller
• Model
• Model EG-1 Econo Gradient Monitor
• Model 2110 Fraction Collector (optional)
• Econo Rack
• Econo System Organizer
• Model SV-3 Diverter Valve
• Model MV-6 6-port Injection Valve
• Model 1325 Econo Recorder (optional)
-1
Econo UV Monitor
EV-1
Econo Buffer Selector
of
the following com-
•
Modell327
The standard Econo System consists
• Model EP-1 Econo Pump
• Model EM-1 Econo UV Monitor
• Model ES-1 Econo System Controller
•
Model2110
• Econo Rack
• Model SV-3 Diverter Valve
• Model MV-6 6-port Injection Valve
• Model 1325 Econo Recorder (optional)
For the Econo System controller to function, it must be connected to the
Model EP-1 Econo Pump. For automated control
Model EV-1 Econo Buffer Selector must be connected to the Econo
controller. Aside from these requirements, UV and gradient monitors, frac-
Econo (Dual-Pen) Recorder (optional)
of
the following components:
Fraction Collector (optional)
of
more than 2 buffers, the
System
2
tion collectors, and chart recorders from other manufacturers can
with the system.
See Appendix A for details.
_Section 2
.~_)npacking
2.1
Unpacking Instructions
Start by removing the solvent bath, which contains the tubing, fittings kit,
and sample injection valve. Next, remove the system controller, by lifting under
of
the base
the plastic bag, and
instrument.
cables, injector valve, and diverter valve. Figure
included with the Econo System controller. Check off all parts against the supplied packing list.
EP-1 Econo Pump, Model EM-1 Econo UV Monitor, etc.) are packaged separately.
If
diately. Refer to Figure
aged part(s).
the instrument. Do not pull on the ribbon cables. Carefully remove
check
Packed next to the instrument is a bag containing the connection
Other components
any parts are missing
for any obvious damage
or
2.1
for the proper designation
be
used
or
problems with the
2.1
shows all the parts
of
the Automated Econo System (Model
damaged, contact Bio-Rad Laboratories imme-
of
the missing
or
dam-
3
Tubing
Fittings Starter Kit
Fraction
Collector_~
Ca~
Chart Recorder
Cable / )
-~
~/
)
Diverter Valve
Injection Valve
Model ES-1 Econo
System Controller
Also includes:
Solvent Reservoir
Instruction Manual w/ Binder
Drain Tube
Fig. 2.1. Parts included with the Model
ES-1
Econo System Controller.
2.2 Voltage Considerations
The Econo System controller receives its power from the Model EP-1
Econo Pump, which is shipped in the
details on voltage conversions to 100 V or 240 V configurations, refer to
the Model
EP-1
Econo Pump manual.
4
120 V or 220 V configuration. For
2.3 Safety Considerations
The Econo System controller receives power from the Model EP-1 Econo
Pump through a ribbon connector shown in Figures 3.3 and 4.2. Do not insert
any objects into this connector.
5
Section
Econo
System
Physical
3.1
t~!~·li'·'·)
A A
3
Controller:
Description
and
Control
Front Panel Functions
ECONO
0 '0
.!\__;\_;\..
SYSTEM
PEAK
COLLECTOR 0 B
CONTROI.I.ER
0 0
Features
GRADIENT FORMER
0
0(~
Fig. 3.1. Front panel controls.
~
~ro]O
Display
Gradient Former
Peak Separator Key
0
~
Function
This control is used to pro-
gram binary gradient meth-
ods consisting
steps in tandem with the
integral
valve and mixer. The gradient LED display gives,
quick identification
dient progression.
This key is used to select
between the two peak separation methods, threshold
detection
dows.
of
proportioning
and
up to five
of
gra-
time
win-
~
(o
o o o o
oO
Time Window
Display and Key
6
This key is used to select
up
to three time windows
for
peak separation. During
each
diverter valve is routed to
the fraction collector for
collection
time
window,
of
eluant.
the
0 0
LIB
Diverter Valve
Indicator and Key
0 0
0
3.2 Rear Panel Functions
~
v
.&
v
~
v
•
LI
This display will indicate
the position
SV-3
Diverter Valve, used
in fraction collection and
peak separation programs.
Diverter valve position can
be manually selected using
the Diverter Valve key.
DIOITM.
CID
~~-~~--~1Y
a
:m:,
11
t234tle7aatou
of
the Model
-.Gil
1 lr
rr:~~
CID
0 0 0
Fig. 3.2. Rear panel functions.
Display Function
Fraction Collector Output This 8-pin mini-DIN sock-
Diverter Valve Output
©
et is used for sending an
advance
Model
Collector
System cable
This 8-pin mini-DIN socket
sends a signal
diverter valve, thus diverting flow from a fraction
collector
device to another receptacle (waste), or vice versa.
signal
2110
vta
or
to
the
Fraction
Econo
1.
to
the
collection
Chart Recorder Output
7
This 8-pin mini-DIN socket
is
used for connection of
the Model
1327
Recorder.
System controller will control the pen lift and paper
feed
Model
1327
Recorder.
1325 or Model
Econo
The
functions
1325
and
Econo
Chart
Econo
of
the
Model
Chart
•
LJ
Waste Port
All spills on the top
Econo System controller
are
led
to a
waste/collection port. From
this port, a drain tube pass-
es out through the rear
the case.
of
the
central
of
10mY
DIGITALCHDSll
FCADVAHCED~
FCADV(•)~
FCADYH+, I I
1 2 3 4 5 6 7 8 9
100mV~1Y
1
~~ANALOGGND
rr.
SHIELD
r--*V3(•)
r+\'3{·)
10
11
110 Connector
3.3 Topside Functions
This 11-pin connector is
used
control
Model
Selector via Econo System
Cable
Econo
manual for details.
This connector is also used
for connecting non-Econo
System
including fraction collectors,
chart
Econo
Appendix A for details.
Dynamic Mixer
for
sending
signals
EV-1
UV
recorders
Econo Buffer
#1
0.
Refer
Buffer
components
monitors,
System.
valve
to the
to the
Selector
and
to
the
See
Fig. 3.3 Top view of the Econo System controller.
8
Gradient Prop. Valve
\
Display
Function
Gradient
Proportioning Valve
Mixer
Ribbon Cables
This valve is used for proportioning two solvents for
the purpose
ear
gradients, as dictated
by
the
control.
Dynamic
rapid
solutes.
These 34- and
bon connectors are for connecting the Model
Econo
Model EP-1 Econo Pump,
respectively, to the Econo
System controller.
is supplied to the system
controller through the
pin Econo Pump connector.
of
forming lin-
Gradient
mixer
mixing
40-pin rib-
UV
Monitor
Former
for
the
of
two
EM-I
and
Power
40-
Section
Econo
System
cold rooms or cold boxes. The orientation
The Econo System Rack is designed to hold the Model EM-1 Econo UV
Monitor optics module, diverter valve, and sample injection valve. The Econo
System Organizer is designed to lift up the Econo System Controller for
placement
Econo Buffer Selector underneath. For this reason, it
Econo Rack and Econo System Organizer be set up before proceeding.
to the underside
details.
4
Controller
The Econo System can be set up in a variety
of
the Model EG-1 Econo Gradient Monitor and Model EV-1
Ribbon cables on the top
of
the pump and UV monitor. See Figures 4.1 and 4.2 for
Connections
of
of
the system controller are used for connection
and
of
locations, including 4
the system is up to the user.
is
recommended that the
Set
Up
oc
9
Econo Pump Econo UV Monitor
Diverter
Valve
G
Power~
Cable
"'<\
Cable
#1
Fig. 4.1. Econo System external connections.
~=911==c=a=bl=e
Model
1325 or
Model 1327 Recorder
Systeif
Controller Power Model
Cable Econo Buffer
=#
::i::::::i=i:::P
2
~c=ab=le=#=1
o::c:::::::::J:D
{ 0 [
EV-1
Selector
J\
4.1
Model EP-1 Econo Pump
When connecting the Model EP-1 Econo Pump to the system controller,
of
the connector on the bottom
I. Gently pull the
exposed
"pump". Do not pull more than 20 em
the pump is used.
connector
out
of
of
cable out
the recessed area labeled
of
the system controller.
2. Holding the connector in one hand, lean the pump forward with the other
hand and make the connection shown in Figure 4.2.
3. Set the pump on the system controller, placing the feet in the areas indicated in Figure 4.2. Make sure the cable feeds back into the system controller. No other connection is required between the pump and system
controller.
10
Fig. 4.2. Connection of Model
Monitor to Econo System controller.
EP-1
Econo Pump
and
Model
EM-1
Econo
UV
4.2 Model
EM-1
Econo UV Monitor
and Optics Module
When connecting the Model
controller, the connector on the bottom
System UV monitor will be used, skip this section and refer to Appendix A.
I. Remove the rubber boot from the recessed area on the system controller,
labeled
2.
Follow steps 1 through 3 in Section 4.I for connection
"UV Monitor".
as shown in Fig. 4.2.
3. Connect the portable optics module to the rear
UV Monitor through the 8-pin circular socket labeled with the optics
module icon.
4. The optics module can be attached, using its feet, to the top
monitor or to the rack. A ring stand adaptor is available for connection
of
the optics module to a ring stand (see Model EM-I Econo UV Monitor
manual).
EM-I
of
Econo UV Monitor to the system
the monitor is used.
of
the Model EM-I Econo
If
a non-Econo
of
the UV Monitor
of
the UV
11
4.3 Model
EV-1
Econo Buffer Selector
and Model SV-5 Valve Pod
The Model
System Controller via Econo System Cable 10. Place the Econo Buffer
Selector control unit underneath the Econo
System Organizer is used) or beside the Econo System Controller. Use cable
#10 (supplied with the Econo Buffer Selector) to connect the Econo
socket on the rear panel
nector on the rear panel
The Model
of
the Solvent Reservoir in a variety
the length
Controller, and the buffer reservoirs. See the Econo Buffer Selector manual
for details.
EV-1
SV-5 Valve Pod can be mounted to the Econo Rack or the lip
of
tubing between the valve pod, the mixer on the Econo System
Econo Buffer Selector connects directly
System Controller (if the Econo
of
the Econo Buffer Selector to the 11-pin 1/0 con-
of
the Econo System Controller.
of
locations. For best results, minimize
to
the Econo
System
4.4 Model EG-1 Econo Gradient Monitor
The Model EG-1 Econo Gradient Monitor connects only to the chart
If
recorder.
Econo Gradient Monitor manual for details.
The Econo Gradient Monitor connects to the Model 1325 Econo (SinglePen) Recorder via Econo
Econo Gradient Monitor) to connect the standard DIN connector on the
Model 1325 Recorder to the 8-pin mini-DIN socket labelled with the chart
recorder icon on the rear panel
conductivity are to be monitored, the Model 1327 (dual-pen) Recorder (or
other dual-channel recording device) should be used. Use Econo
Cable 4 (supplied with the Econo Gradient Monitor, 8-pin mini-DIN to
banana plugs) to connect the Econo Gradient Monitor to the banana plug
signal inputs on the chart recorder. Plug the 8-pin mini-DIN end
to the chart recorder socket on the rear panel
Insert the black plug into the ground
voltage
a non-Econo Recorder is to be used, refer to the Model EG-1
System Cable
of
V input for channel 2
the Econo Gradient Monitor.If both UV and
of
2.
Use cable 2 (supplied with the
of
of
the Econo Gradient Monitor.
_l_ input and the red plug into the
the Model 1327 Econo Recorder.
System
cable 4
4.5 Model
Interfacing between the Model2110 Fraction Collector and system controller is achieved by connecting the 9-pin
Model2110 Fraction Collector to the 8-pin mini-DIN socket labeled with the
fraction collector icon on the rear panel of the system controller (refer to
Figure 3.2).
Controller) is used to make this connection. The fraction collector can also
be connected to the 8-pin mini-DIN socket labeled with the fraction collec-
211
Econo
0 Fraction Collector
1/0 connector on the back
System
Cable
1 (supplied with the Econo
of
the
System
12
tor icon on the rear
System
fraction collector, refer to Appendix
of
the Model EP-1 Econo Pump. To connect a non Econo ·
A.
4.6 Model SV-3 Diverter Valve
The Model SV-3 Diverter Valve (Figure 4.3) can be attached to the rack
of
in a variety
are labeled to indicate their functions. The cable for the diverter valve is
attached to the back
et
labeled with the diverter valve icon (refer to Figure 3.2). The diverter
valve can also be connected to the 8-pin mini-DIN socket labeled with the
Diverter Valve icon on the rear
locations depending upon the application. The ports on the valve
of
the system controller using the 8-pin mini-DIN sock-
of
the Model EP-1 Econo Pump.
Fig. 4.3. Model
SV-3
Diverter Valve.
4. 7 Solvent Reservoir
The solvent reservoir sits in the depression in the system controller behind
the pump and
UV monitor.
Be
careful not to trap cables under the reservoir.
4.8 Model 1325 Econo (Single-Pen) Recorder
The Model 1325 Econo Recorder has been designed for use with the
Econo
analog data signal, pen drop, and paper feed command to the recorder. Refer
to the recorder instruction manual for operating details.
1325 Econo Recorder
plied with the system controller to connect the standard DIN connector on the
Model 1325 Econo Recorder to the 8-pin mini-DIN socket labeled with the
chart recorder icon on the rear panel
3.2).
tings labeled in green for automatic control.
System. Through one connection, the system controller will output the
To
connect the Model
to
the system controller, use Econo System Cable 2 sup-
of
the system controller (see Figure
Set all top panel controls
of
the Model1325 Econo Recorder to the set-
4.9 Model 1327 (Dual-Pen) Econo Recorder
The Model 1327 Econo Recorder has been designed for use with the
Econo
analog data signal, pen drop, and paper feed commands to the recorder.
System. Through one connection, the system controller will output the
13
To
connect the Model 1327 Econo Recorder to the system controller, use Econo
System Cable 2 (mini-DIN to standard-DIN) to connect the standard-DIN connector on the Model 1327 Econo Recorder to the 8-pin mini-DIN socket
~
labelled with the
Figure 3.2).
icon on the rear panel
of
the system controller (See
4.10 Data Acquisition Systems
The system controller takes into account the use
data acquisition systems, such as the Model 3396A Integrator, or other instruments. The
Econo
Econo System Cable 7. Alternatively,
connected to the System Controller, the 11-pin connector on the rear panel
of
the system controller can be used. See Appendix A for signal specifications.
Section
5
Plumbing
The Econo System can be plumbed quickly and easily. Twenty-five feet
of
Tygon® tubing and a starter tubing fittings kit are included with the system. The kit contains luer fittings for quick tubing connections. Figure 5.1
shows a typical plumbing diagram for the Automated Econo System. Figure
5.2 shows a typical plumbing diagram for the Econo System without the
Econo Buffer Selector and Econo Gradient Monitor.
of
the Econo System with
~
8-pin mini-DIN socket on the rear panel
UV Monitor will output a non-scaled analog signal, accessible through
if
the Econo Buffer Selector is not
the
System
of
the Model EM-I
u
Fig. 5.1. Plumbing diagram for the Automated Econo System.
14
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