Bio-rad Econo System User Manual

Econo System

Instruction

Manual

For

Technical Service

Call Your Local Bio-Rad Office

in the U.S.

t=it•J;1

Calll-800-4BIORAD

( 1-800-424-6723)

9

•)

or

Warranty

Model Serial Number

Date Warranty Period

Unless otherwise specified, instruments sold by Bio-Rad Laboratories are

warranted for 1 year against defects in materials and workmanship.

If

replace the defective parts without charge. However, the following defects are specifically excluded:

1.

Defects caused by improper operation.

2.

Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized agent.

3.

Use with tubings or fittings not specified by Bio-Rad Laboratories for use with this system.

4. Deliberate

_______________

______________

of

Delivery

any defects should occur during this warranty period, Bio-Rad will

or

-------------

____________

accidental misuse.

_

5.

Damage caused by disaster.

6.

Damage due to use

7. Damage due to spills. This warranty does not apply to tubing, fittings, and fuses. For inquiry or request for repair service, contact Bio-Rad Laboratories after

confirming the model and serial number

For Technical

Call l-800-4BIORAD (1-800-424-6723)

of

improper solvents or samples.

of

your instrument.

Service Call Your Local Bio-Rad Office

or

in the U.S.

Table

of

Contents

Section 1

1.1

1.2

Section2

2.1

2.2

2.3

Section 3

3.1

3.2

3.3

Section 4

4.1

4.2

4.3

4.4

4.5

4.6

4.7

4.8

4.9

4.10

Introduction .................................................................. .

Overview ................................................................................. .

System Components ................................................................ .

Unpacking the Econo System Controller ................... .

Unpacking Instructions ........................................................... .

Voltage Considerations ............................................................ .

Safety Considerations .............................................................. .

1

2

3

4

5

Econo System Controller:

Physical Description and Control Features ............... .

Front Panel Functions .............................................................. .

Rear

Panel Functions ............................................................... .

Topside Functions .................................................................... .

6

7

8

Econo System Controller Connections and Set Up .... 9

Model EP-1 Econo Pump......................................................... 10

Model EM-1 Econo UV Monitor and Optics Module.............

Model

and Model

Model EG-1 Econo Gradient Monitor . . . .... ... . . ...

Model Model

Solvent Reservoir.....................................................................

Model 1325 Econo (Single-pen) Recorder...............................

Model 1327 Econo (Dual-pen) Recorder.................................

Data Aquisition Systems ............................ .............................. 14

EV-1

2110 Fraction Collector SV-3

Econo Buffer Selector

SV-5

Valve Pod......................................................

Diverter Valve......................................................

..

. . ...

...

. .......

..

. .

.. ..

...

. .

...

. . ... . .

.....

..

. . .... ...... ... 12

..

11

12

. . . . . 12

13 13 13 13

Section 5

5.1

5.2

5.3

5.4

5.5

5.6

5.7

5.8

Section 6

6.1

6.2

Plumbing the System..................................................... 14

Tubing and Fittings . .

Gradient Proportioning Valve and Mixer................................. 16

Model Model

Manual Sample Injection Valve

and Chromatography Column . . . ... . .

UV Optics Module and Gradient Monitor Flow Cell . . . .

Model

Fraction Collector.....................................................................

EP-1 Econo Pump......................................................... 16

SV-5

Valve Pod.............................................................

SV-3

Diverter Valve ......................................................

..

... . . . . ..... ..... ... . .........

... . ..

.. . ..

. ........ ...........

. . ..... ... . .

.. . ..

..

.. . ..

. . .

..

. . ...

15

17

.. . .. . .. . 18

.. . .. ..

... 20

21 21

Getting Started .............................................................. 21

Connecting System to Power

System Check........................................................................... 22

..

. . . ..... ... . . ... ...... ....

..

. . .

..

......

...

..

...

21

Section 7

7.1

7.2

7.3

7.4

7.5

7.6

7.7

7.8

7.9

Programming the System .............................................

Getting Started:

Verification

Econo

!socratic (Single Buffer) Methods............................................

Binary Gradient Formation . ... . . . . .

Peak Detection and Collection .....

Programming Multi-Step Methods

(Using the Econo Buffer Selector)........................................... 35

Programming Multi-Step Methods Which Include a Binary

Gradient (Using the Econo Buffer Selector)............................ 38

Programming Automated

Options While Running A Method . . ..... ...

of

Econo System Software Version...................... 23

System Software Mode Selection................................. 24

..

. .

...

. .....

...

. . ...

..

... . ... . . . .... ...

.. . ...

......

..

......

...

..

... ....... ....

Sample Injection............................. 39

..

.... ... . . .... ... . . .

..

. . .

..

. .

... ...

..

23

25 28 31

40

Section 8

Section 9 Appendix A Appendix B Appendix C

Storage and Maintenance............................................. 42

Thoubleshooting ............................................................. 43

Interfacing and Signals .................................................

45

System Specifications .................................................... 47

Ordering Information................................................... 48

Section

1

Introduction

Fig. 1.1. The Automated Econo System.

1.1

Overview

The Automated Econo System consists

matography instruments which can be integrated to form a system. System components include a complete line cartridges, fittings, tubing, and valves. Each component has its own stand-alone features in addition to its role in the system.

The Model ES-1 Econo System Controller integrates the individual com-

ponents into a low pressure chromatography system. The Econo System con-

troller provides the following functions:

• Gradient proportioning and mixing

• Peak detection by threshold

• Programmed control Buffer Selector is used)

• Waste/collect diverter valve

• 6-port manual injector valve

of

up to 5 solutions (when the Model

of

or

time windows

of

a series

chromatography columns, prepacked

of

free-standing chro-

EV-1

Econo

• Solvent ice bath

These functions supplement those provided by the Model EP-1 Econo Pump, Model EM-1 Monitor, Model EV-1 Econo Buffer Selector, and Model Collector. This manual contains the necessary information for setting up and

Econo

UV

Monitor, Model EG-1 Econo Gradient

2110 Fraction

1

running the system. In some sections, references will be made to informati1 provided in the component manuals. A binder is provided to help organi

the information.

1.2 System Components

The complete Automated Econo System consists

ponents:

• Model EP-1 Econo Pump

• Model EM

• Model ES-1 Econo System Controller

• Model

• Model EG-1 Econo Gradient Monitor

• Model 2110 Fraction Collector (optional)

• Econo Rack

• Econo System Organizer

• Model SV-3 Diverter Valve

• Model MV-6 6-port Injection Valve

• Model 1325 Econo Recorder (optional)

-1

Econo UV Monitor

EV-1

Econo Buffer Selector

of

the following com-

•

Modell327

The standard Econo System consists

• Model EP-1 Econo Pump

• Model EM-1 Econo UV Monitor

• Model ES-1 Econo System Controller

•

Model2110

• Econo Rack

• Model SV-3 Diverter Valve

• Model MV-6 6-port Injection Valve

• Model 1325 Econo Recorder (optional) For the Econo System controller to function, it must be connected to the

Model EP-1 Econo Pump. For automated control Model EV-1 Econo Buffer Selector must be connected to the Econo controller. Aside from these requirements, UV and gradient monitors, frac-

Econo (Dual-Pen) Recorder (optional)

of

the following components:

Fraction Collector (optional)

of

more than 2 buffers, the

System

2

tion collectors, and chart recorders from other manufacturers can with the system.

See Appendix A for details.

_Section 2

.~_)npacking

2.1

Unpacking Instructions

Start by removing the solvent bath, which contains the tubing, fittings kit,

and sample injection valve. Next, remove the system controller, by lifting under

of

the base the plastic bag, and instrument. cables, injector valve, and diverter valve. Figure included with the Econo System controller. Check off all parts against the supplied packing list. EP-1 Econo Pump, Model EM-1 Econo UV Monitor, etc.) are packaged separately.

If

diately. Refer to Figure aged part(s).

the instrument. Do not pull on the ribbon cables. Carefully remove

check

Packed next to the instrument is a bag containing the connection

Other components

any parts are missing

for any obvious damage

or

2.1

for the proper designation

be

used

or

problems with the

2.1

shows all the parts

of

the Automated Econo System (Model

damaged, contact Bio-Rad Laboratories imme-

of

the missing

or

dam-

3

Tubing

Fittings Starter Kit

Fraction

Collector_~

Ca~

Chart Recorder

Cable / )

-~

~/

)

Diverter Valve

Injection Valve

Model ES-1 Econo System Controller

Also includes:

Solvent Reservoir Instruction Manual w/ Binder Drain Tube

Fig. 2.1. Parts included with the Model

ES-1

Econo System Controller.

2.2 Voltage Considerations

The Econo System controller receives its power from the Model EP-1 Econo Pump, which is shipped in the details on voltage conversions to 100 V or 240 V configurations, refer to the Model

EP-1

Econo Pump manual.

4

120 V or 220 V configuration. For

2.3 Safety Considerations

The Econo System controller receives power from the Model EP-1 Econo Pump through a ribbon connector shown in Figures 3.3 and 4.2. Do not insert any objects into this connector.

5

Section Econo

System

Physical

3.1

t~!~·li'·'·)

A A

3

Controller:

Description

and

Control

Front Panel Functions

ECONO

0 '0

.!\__;\_;\..

SYSTEM

PEAK

COLLECTOR 0 B

CONTROI.I.ER

0 0

Features

GRADIENT FORMER

0

0(~

Fig. 3.1. Front panel controls.

~

~ro]O

Display

Gradient Former

Peak Separator Key

0

~

Function

This control is used to pro-

gram binary gradient meth-

ods consisting steps in tandem with the integral

valve and mixer. The gradient LED display gives, quick identification dient progression.

This key is used to select between the two peak separation methods, threshold detection dows.

of

proportioning

and

up to five

of

gra-

time

win-

~

(o

o o o o

oO

Time Window Display and Key

6

This key is used to select

up

to three time windows

for

peak separation. During

each diverter valve is routed to the fraction collector for collection

time

window,

of

eluant.

the

0 0

LIB

Diverter Valve Indicator and Key

0 0

0

3.2 Rear Panel Functions

~

v

.&

v

~

v

•

LI

This display will indicate the position SV-3

Diverter Valve, used in fraction collection and peak separation programs. Diverter valve position can be manually selected using the Diverter Valve key.

DIOITM.

CID

~~-~~--~1Y

a

:m:,

11

t234tle7aatou

of

the Model

-.Gil

1 lr

rr:~~

CID

0 0 0

Fig. 3.2. Rear panel functions.

Display Function

Fraction Collector Output This 8-pin mini-DIN sock-

Diverter Valve Output

©

et is used for sending an advance Model Collector System cable

This 8-pin mini-DIN socket

sends a signal diverter valve, thus diverting flow from a fraction

collector device to another receptacle (waste), or vice versa.

signal

2110

vta

or

to

the

Fraction

Econo

1.

to

the

collection

Chart Recorder Output

7

This 8-pin mini-DIN socket

is

used for connection of

the Model

1327

Recorder. System controller will control the pen lift and paper

feed Model

1327

Recorder.

1325 or Model

Econo

The

functions

1325

and

Econo

Chart

Econo

of

the

Model

Chart

•

LJ

Waste Port

All spills on the top Econo System controller are

led

to a waste/collection port. From this port, a drain tube pass-

es out through the rear the case.

of

the

central

of

10mY

DIGITALCHDSll

FCADVAHCED~

FCADV(•)~

FCADYH+, I I

1 2 3 4 5 6 7 8 9

100mV~1Y

1

~~ANALOGGND

rr.

SHIELD

r--*V3(•)

r+\'3{·)

10

11

110 Connector

3.3 Topside Functions

This 11-pin connector is used control Model

Selector via Econo System Cable Econo manual for details.

This connector is also used for connecting non-Econo System including fraction collectors, chart Econo Appendix A for details.

Dynamic Mixer

for

sending

signals

EV-1

UV

recorders

Econo Buffer

#1

0.

Refer

Buffer

components

monitors,

System.

valve

to the

Selector

and

to

the

See

Fig. 3.3 Top view of the Econo System controller.

8

Gradient Prop. Valve

\

Display

Function

Gradient Proportioning Valve

Mixer

Ribbon Cables

This valve is used for proportioning two solvents for the purpose ear

gradients, as dictated

by

the

control. Dynamic

rapid solutes.

These 34- and bon connectors are for connecting the Model Econo Model EP-1 Econo Pump,

respectively, to the Econo System controller. is supplied to the system controller through the pin Econo Pump connector.

of

forming lin-

Gradient

mixer

mixing

40-pin rib-

UV

Monitor

Former

for

the

of

two

EM-I

and

Power

40-

Section Econo

System

cold rooms or cold boxes. The orientation The Econo System Rack is designed to hold the Model EM-1 Econo UV Monitor optics module, diverter valve, and sample injection valve. The Econo System Organizer is designed to lift up the Econo System Controller for placement Econo Buffer Selector underneath. For this reason, it Econo Rack and Econo System Organizer be set up before proceeding.

to the underside details.

4

Controller

The Econo System can be set up in a variety

of

the Model EG-1 Econo Gradient Monitor and Model EV-1

Ribbon cables on the top

of

the pump and UV monitor. See Figures 4.1 and 4.2 for

Connections

of

the system controller are used for connection

and

of

locations, including 4

the system is up to the user.

is

recommended that the

Set

Up

oc

9

Econo Pump Econo UV Monitor

Diverter

Valve

G

Power~

Cable

"'<\

Cable

#1

Fig. 4.1. Econo System external connections.

~=911==c=a=bl=e

Model

1325 or

Model 1327 Recorder

Systeif

Controller Power Model

Cable Econo Buffer

=#

::i::::::i=i:::P

2

~c=ab=le=#=1

o::c:::::::::J:D

{ 0 [

EV-1

Selector

J\

4.1

Model EP-1 Econo Pump

When connecting the Model EP-1 Econo Pump to the system controller,

of

the connector on the bottom

I. Gently pull the

exposed

"pump". Do not pull more than 20 em

the pump is used.

connector

out

of

cable out

the recessed area labeled

of

the system controller.

2. Holding the connector in one hand, lean the pump forward with the other hand and make the connection shown in Figure 4.2.

3. Set the pump on the system controller, placing the feet in the areas indicated in Figure 4.2. Make sure the cable feeds back into the system controller. No other connection is required between the pump and system

controller.

10

Fig. 4.2. Connection of Model

Monitor to Econo System controller.

EP-1

Econo Pump

and

Model

EM-1

Econo

UV

4.2 Model

EM-1

Econo UV Monitor

and Optics Module

When connecting the Model controller, the connector on the bottom System UV monitor will be used, skip this section and refer to Appendix A.

I. Remove the rubber boot from the recessed area on the system controller,

labeled

2.

Follow steps 1 through 3 in Section 4.I for connection

"UV Monitor".

as shown in Fig. 4.2.

3. Connect the portable optics module to the rear UV Monitor through the 8-pin circular socket labeled with the optics module icon.

4. The optics module can be attached, using its feet, to the top monitor or to the rack. A ring stand adaptor is available for connection of

the optics module to a ring stand (see Model EM-I Econo UV Monitor

manual).

EM-I

of

Econo UV Monitor to the system

the monitor is used.

of

the Model EM-I Econo

If

a non-Econo

of

the UV Monitor

of

the UV

11

4.3 Model

EV-1

Econo Buffer Selector

and Model SV-5 Valve Pod

The Model System Controller via Econo System Cable 10. Place the Econo Buffer Selector control unit underneath the Econo System Organizer is used) or beside the Econo System Controller. Use cable #10 (supplied with the Econo Buffer Selector) to connect the Econo socket on the rear panel nector on the rear panel

The Model of

the Solvent Reservoir in a variety the length Controller, and the buffer reservoirs. See the Econo Buffer Selector manual for details.

EV-1

SV-5 Valve Pod can be mounted to the Econo Rack or the lip

of

tubing between the valve pod, the mixer on the Econo System

Econo Buffer Selector connects directly

System Controller (if the Econo

of

the Econo Buffer Selector to the 11-pin 1/0 con-

of

the Econo System Controller.

of

locations. For best results, minimize

to

the Econo

System

4.4 Model EG-1 Econo Gradient Monitor

The Model EG-1 Econo Gradient Monitor connects only to the chart

If

recorder. Econo Gradient Monitor manual for details.

The Econo Gradient Monitor connects to the Model 1325 Econo (SinglePen) Recorder via Econo Econo Gradient Monitor) to connect the standard DIN connector on the Model 1325 Recorder to the 8-pin mini-DIN socket labelled with the chart recorder icon on the rear panel conductivity are to be monitored, the Model 1327 (dual-pen) Recorder (or

other dual-channel recording device) should be used. Use Econo Cable 4 (supplied with the Econo Gradient Monitor, 8-pin mini-DIN to

banana plugs) to connect the Econo Gradient Monitor to the banana plug

signal inputs on the chart recorder. Plug the 8-pin mini-DIN end to the chart recorder socket on the rear panel Insert the black plug into the ground voltage

a non-Econo Recorder is to be used, refer to the Model EG-1

System Cable

of

V input for channel 2

the Econo Gradient Monitor.If both UV and

of

2.

Use cable 2 (supplied with the

of

the Econo Gradient Monitor.

_l_ input and the red plug into the

the Model 1327 Econo Recorder.

System

cable 4

4.5 Model

Interfacing between the Model2110 Fraction Collector and system controller is achieved by connecting the 9-pin Model2110 Fraction Collector to the 8-pin mini-DIN socket labeled with the fraction collector icon on the rear panel of the system controller (refer to Figure 3.2). Controller) is used to make this connection. The fraction collector can also be connected to the 8-pin mini-DIN socket labeled with the fraction collec-

211

Econo

0 Fraction Collector

1/0 connector on the back

System

Cable

1 (supplied with the Econo

of

the

System

12

tor icon on the rear System

fraction collector, refer to Appendix

of

the Model EP-1 Econo Pump. To connect a non Econo ·

A.

4.6 Model SV-3 Diverter Valve

The Model SV-3 Diverter Valve (Figure 4.3) can be attached to the rack

of

in a variety are labeled to indicate their functions. The cable for the diverter valve is attached to the back et

labeled with the diverter valve icon (refer to Figure 3.2). The diverter valve can also be connected to the 8-pin mini-DIN socket labeled with the Diverter Valve icon on the rear

locations depending upon the application. The ports on the valve

of

the system controller using the 8-pin mini-DIN sock-

of

the Model EP-1 Econo Pump.

Fig. 4.3. Model

SV-3

Diverter Valve.

4. 7 Solvent Reservoir

The solvent reservoir sits in the depression in the system controller behind

the pump and

UV monitor.

Be

careful not to trap cables under the reservoir.

4.8 Model 1325 Econo (Single-Pen) Recorder

The Model 1325 Econo Recorder has been designed for use with the Econo analog data signal, pen drop, and paper feed command to the recorder. Refer to the recorder instruction manual for operating details.

1325 Econo Recorder plied with the system controller to connect the standard DIN connector on the Model 1325 Econo Recorder to the 8-pin mini-DIN socket labeled with the chart recorder icon on the rear panel

3.2). tings labeled in green for automatic control.

System. Through one connection, the system controller will output the

To

connect the Model

to

the system controller, use Econo System Cable 2 sup-

of

the system controller (see Figure

Set all top panel controls

of

the Model1325 Econo Recorder to the set-

4.9 Model 1327 (Dual-Pen) Econo Recorder

The Model 1327 Econo Recorder has been designed for use with the Econo analog data signal, pen drop, and paper feed commands to the recorder.

System. Through one connection, the system controller will output the

13

To

connect the Model 1327 Econo Recorder to the system controller, use Econo System Cable 2 (mini-DIN to standard-DIN) to connect the standard-DIN connector on the Model 1327 Econo Recorder to the 8-pin mini-DIN socket

~

labelled with the Figure 3.2).

icon on the rear panel

of

the system controller (See

4.10 Data Acquisition Systems

The system controller takes into account the use data acquisition systems, such as the Model 3396A Integrator, or other instruments. The Econo Econo System Cable 7. Alternatively, connected to the System Controller, the 11-pin connector on the rear panel of

the system controller can be used. See Appendix A for signal specifications.

Section

5

Plumbing

The Econo System can be plumbed quickly and easily. Twenty-five feet of

Tygon® tubing and a starter tubing fittings kit are included with the system. The kit contains luer fittings for quick tubing connections. Figure 5.1 shows a typical plumbing diagram for the Automated Econo System. Figure

5.2 shows a typical plumbing diagram for the Econo System without the

Econo Buffer Selector and Econo Gradient Monitor.

of

the Econo System with

~

8-pin mini-DIN socket on the rear panel

UV Monitor will output a non-scaled analog signal, accessible through

if

the Econo Buffer Selector is not

the

System

of

the Model EM-I

u

Fig. 5.1. Plumbing diagram for the Automated Econo System.

14

u

Fig. 5.2. Plumbing diagram for the Econo System (without Econo Buffer Selector

and Econo Gradient Monitor).

The plumbing arrangement described in this section is by no means the only way to connect the system. The additional tubing and fittings are supplied to allow the system to be customized for specific applications.

5.1

Tubing and Fittings

The Econo System can be conveniently plumbed with most tubing having inner diameters less than or equal to 3.2 mm

supplied in the plumbing kit is 1.6 mm ID. This should suffice for most applications. When using 3.2 mm tubing in the pump for increased pump output, the proportioning valve and mixer should also be plumbed with 3.2

flow.

mm tubing to maintain adequate manual for tubing compatibilities with the pump.) Figure 5.3 summarizes the more commonly used fittings.

(Refer to the Model EP-1 Econo Pump

(Ys").

The Tygon tubing

Male luer for system tubing connections

Female luer for pump tubing

.,.

Female 't' connector

1 Male-to-male connector

Fig. 5.3. Fittings for system tubing connections.

15

Note: Tubing lock-rings are used only for plumbing the Model EP-1

Econo Pump and are not needed for system tubing connections.

5.2 Gradient Proportioning Valve and Mixer

The length valve is not critical; however, it is important be designated buffer A and buffer B.

1.

Insert the barbed end

Connect the male luer fitting to the female luer fitting on the propor-

tioning valve. Make sure that the line from buffer A is connected to the A port on the proportioning valve. Repeat this process for buffer line B. Do not overtighten the luer fittings.

2. The connection between the proportioning valve and mixer should be made with a piece accuracy luer fittings the outlet Figure 5.1).

5.3 Model

Installation

EP-1 Econo Pump manual. Pre-cut norprene tubing and fittings are supplied

with the pump. tubing, refer to the following procedure. Note that tubing length varies with tubing composition. This is to accommodate tubing prestretch, which affects flow rate calibration and flow reproducibility.

of

tubing between the buffer bottles and the proportioning

to

clearly label which bottles will

of

a male luer fitting into one end

of

tubing less than

of

the gradients formed by the proportioning valve. Affix male to

both ends

of

the proportioning valve with the inlet

EP-1

of

To

Econo Pump

the tubing in the pump head is described in the Model

make these tubing sections yourself with Tygon, or other

of

the tubing as described above, and connect

10

em long. This

of

the tubing.

is

to maintain the

of

the mixer (see

Warning:

sure the wall thickness is not greater then greater wall thickness can damage the pump and void your warranty.

1.

Cut the tubing to a length described below. Make sharp, perpendicular cuts.

Tubing

Tygon, Norprene Silicone

2.

Slip a color-coded lock-ring specific for that size

of

the tubing (see below). Install the female

barbed end

place by sliding the lock-ring over the barbed fitting (see Figure 5.4). Repeat on the other end

If

using tubing other than the type supplied by Bio-Rad, make

0.8 mm. Using tubing with a

Length

179

mm

+1-

1.3 (7.04"

171

mm

+1-

1.3 (6.75"

of

the fitting into the tubing, and clamp the luer fitting into

of

the tubing.

16

+1-

0.05)

+1-

0.05)

of

tubing onto one end

1uer

fittings by inserting the

Lock-Ring Color

Size

Red Orange Yellow

If

a Model

EV-1

Econo Buffer Selector is not being used, the connection between the pump and the mixer should be made with a section then

15

em long to maintain accuracy

0.8 mm

1.6

3.2 mm

of

gradients. Affix male luer fittings

mm

(~2")

(YI6")

(Ys")

of

tubing less

to both ends as described above, and connect to the female luer fittings on both the pump outlet and the mixer.

If

a Model

mixer to the pump. Continue with

EV-1

Econo Buffer Selector is to be used, do not connect the

Section 5.4 for plumbing the Model SV-5

Valve Pod.

Fig. 5.4. Attachment of tubing fittings.

5.4 Valve Pod

The Model SV-5 Valve Pod should be positioned as close as possible to the mixer on the Econo of

tubing should be should already be plumbed through the proportioning valve and mixer on the Econo the Econo

System Controller. Connect tubing from the outlet

System controller to the Pod. Connect tubing from each buffer/sample reservoir (C, D, E) to the corresponding valve port on the Model the outlet port

of

the valve pod to the Model EP-1 Econo Pump.

System controller and the Econo Pump. The length

of

minimal length and inner diameter. Buffers A and B

of

the mixer on

AlB

inlet port on the Model SV-5 Valve

SV-5 Valve Pod. Connect tubing

17

fro~

5.5 Manual Sample Injection Valve and Chromatography Column

Samples can be introduced into the flow path

of

variety below. tem, post-pump; the other allows pre-pump variable injection volumes. The MV-6 injector valve three-way valve can be inserted in-line between the pump and the column inlet or

between the pump and mixer to provide a simple injection port. For details

on automated sample injection, see Section 7.8.

ways. Two methods for manual sample injection are described

One uses preset injection volumes with a classical injection loop sys-

can

be used in both configurations. Alternatively, a

of

the Econo System in a

18

Post-Pump Injection with a Sample Loop

1.

The Model MV-6 Injector Valve can be installed in a variety

tions on the rack.

Use the screw fitting on the injector valve housing to

of

loca-

secure the valve.

of

2. Cut a section

tubing long enough to connect the pump and the injec-

tor port. Affix male luer fittings as described above.

of

3. An injection loop of

tubing

of

a length which corresponds to a desired volume. Table 5.1

a desired volume can be formed by using a section

provides the tubing lengths that correspond to several common loop volumes.

of

4. Attach a male luer-fitting to each end

the sample loop tube.

5. Connect the inlet line from the pump, sample loop, and outlet line to the

column

as

shown in Figure 5.4. Arrow indicates rotation direction of valve.

Waste

Sample Loop

~

Pump

Fig. 5.5. Tubing connection for post-pump injector valve placement.

Pre-Pump Injection

1.

Install the injector valve on the rack in a location close to the mixer.

2. Disconnect the tubing between the pump and mixer.

3. Connect the mixer to the injector valve, and the injection valve to the pump, as shown in Figure 5.5. Arrow indicates rotation direction valve.

Column

of

19

Mixer

Sample

Econo Pump

3-way Stopcock

Fig. 5.6. Tubing connection for

Table

Tubing

0.8mm

1.6mm

1.6

1.6mm

3.2mm

mm

5.1

Sample Injection Loop Volumes by Tubing ID

10

pre-pump

Loop Tubing Volume

100

200

500

1

500

1

2ml

5ml

injector valve placement.

Jll

ml

Jll

ml

Length

14

em

34em

8.5em

23.5 em

48.5 em

5.9em

12.1

em

24.6 em

62.1

em

Note: Luer fittings and valve internal passages add approximately 60 above volumes.

J..l].

to

5.6 UV Optics Module and Gradient Monitor Flow Cell

The Model EM-I Econo as close to the column outlet as possible, to minimize mixing sample prior to detection. The tubing end used to connect the outlet matography column should have a female luer fitting attached. Arrows on the

of

top

the optics module are used to indicate flow direction. The tubing ends to be connected to the inlet affixed with a male luer fitting. It is important to minimize the length and inner

of

diameter

this section

of

tubing.

UV

Monitor optics module should

and

outlet lines

of

the

UV

20

be

coupled

of

the separated

of

the chro-

monitor should

be

Connect the Model EG-1 Econo Gradient Monitor flow cell directly to

or

the inlet

detection.

Warning: Do not overtighten the luer fittings.

5.

7 Model SV-3 Diverter Valve

The Model SV-3 Diverter Valve should be coupled as close as possible to the UV monitor outlet. The length length and inner diameter. Connect two sections fitting on one end and connect to both the collect and waste ports diverter valve. Direct the tubing from the waste port to an appropriate waste container. Connect the tubing from the collector port to a fraction collector or suitable collection device as described in Section 5.8.

outlet port

of

the UV optics module for optimal conductivity

of

tubing used should be

of

tubing with a male luer

of

minimal

of

the

5.8 Fraction Collector

To

connect the diverter valve to the Model2110 Fraction Collector, affix a male luer fitting to one end to the female luer fitting to the collection port Place the other end drop forming arm. See the Model

ual for details on this connection. Minimizing the length this connection will improve the accuracy with which peaks are collected.

of

the tubing into the rubber ferrule

of

a length

2110 Fraction Collector instruction man-

of

tubing, and attach this connector

of

the flow diverter valve.

of

the fraction collector

of

tubing used for

Section Getting

It

is

ule. Refer to Section 2.2

6.1

1.

2.

3.

4. Model EG-1 Econo Gradient Monitor (requires power adaptor)

5.

6. Model 1325

6

Started

After the Econo System is connected and plumbed, it can be powered up. important that the input voltage match the voltage on the power entry mod-

if

it does not match.

Connecting System to Power

The following line cords need to be connected to power up the system. Model EP-1 Econo Pump Model EM-1 Econo UV Monitor Model

Model 2110 Fraction Collector

power adaptor)

EV-1

Econo Buffer Selector (requires power adaptor)

or

Model 1327 Econo Recorder (optional, both require

21

The Model EP-1 Econo Pump will supply the power to the system controller through the ribbon cable connected to the pump base. The Model EM-1 Econo the gradient monitor, buffer selector, chart recorder and fraction collector. The UV monitor is powered up with the lamp on. Ensure that the optics module is plugged into the appropriate socket on the rear panel The

15

warm up.

UV Monitor is plugged directly into a grounded power source, as are

of

the UV monitor.

UV lamp LED will flash during the first minute

minutes for the UV lamp, and 10 minutes for the gradient monitor to

or

two. Allow at least

6.2 System Check

Before proceeding, it is advisable to check the system for leaks or other

problems. reservoir containing water. Disconnect any chromatography column during the test.

To

carry out the test, place buffer lines for buffer A and B into a

0 0

LJ

• 0

0

~

1.

2.

3.

4.

Press the Run/Stop key on the pump front panel.

The run and % light will come on, and the pump display will read

Press the Waste/Collect key on the system controller

front panel until the waste light is lit. When the valve cycles between waste and collect, a click should be

If

heard. controller.

Using the scroll keys on the pump, increase the pump

output to 25%. The pump head should begin to tum.

Over the next 10 minutes, watch for any leaks

bubbles.

tered, press Run/Stop to stop the pump, and repair the leak and remove air bubbles. Restart the pump.

not, check the connection with the system

If

leaks

0.

or

excessive bubbles are encoun-

or

air

22

5.

Press the Purge key on the pump front panel and let

pump operate at buffer A for several minutes. Again, check for any leaks in the system. When purging, the diverter valve

be

should

in the waste position.

100% maximum output

(25

rpm)

of

6.

7.

To prime with

Former key (see Figure 3.1) while in the Purge mode. The Gradient Former indicator light will flash and the pump display will show a flashing

100% buffer B output.

Note:

E (if the Econo Buffer Selector is being used), the

system must be configured in the Enhanced mode

(See Section 7 .1). Buffers A through E can then be

selected by depressing the Gradient Former key.

Release the key when the desired buffer

on the Econo Buffer Selector display. The valves

will not change until the key is released.

Press the Run/Stop key to stop the pump,

Purge key once more to put the pump into Run mode.

To

prime the buffer lines for buffers C, D, and

100% buffer B, press the Gradient

"b" to indicate

is

displayed

or

the

Section

7

Programming

The Econo System components function in the system configuration much the same way they do in the stand-alone configuration. For more specific details on the operation uals supplied with them. This section summarizes the function in various separation strategies, and various levels

7.1

Getting Started: Verification of Econo System

Software Version

The Model EP-1 Econo Pump must have software version 2.01 or high-

er

to control the Model EV-1 Econo Buffer Selector.

include a Model does include a Model sion

of

your system before proceeding:

the

EV-1

System

of

the individual components, refer to the man-

of

If

Econo Buffer Selector, see Section 7 EV-1

Econo Buffer Selector, check the software ver-

of

the system

complexity.

your system does not

.2.

If

your system

23

Simultaneously press and hold the Direction Key and the Model EP-1 Econo Pump. The four digit LED display on the front panel play:

If

your Econo Pump displays a number lower than 2.01. it must be upgraded. Call your local representative for an EP-1 ROM Replacement Kit. Disconnect the Econo Pump from all connections and replace the ROM following the directions in the procedure takes about replacement, please contact your local Bio-Rad representative.

If

your pump currently features software version 2.01 or higher, ROM upgrade has already been completed, ensure that all connections between the Econo Pump, Econo have been completed and the system power is on.

15

"down" Arrow key on the front panel

of

the Econo Pump should dis-

"v2.01"

ROM Replacement Kit. The ROM replacement

minutes.

If

you do not wish to perform the ROM

System Controller and Econo Buffer Selector

or

of

if

the

7.2 Econo System Software Mode Selection

Software version 2.01 and higher features two user-selectable programming modes, Standard and Enhanced. The "Enhanced" software mode is required to use the Model choice whenever more than two buffers will be controlled, methods longer than 5 steps or automated sample injection is desired. The "Standard" soft-

is

ware mode 5 steps, including simple binary gradient methods.

the mode

EV-1

of

choice for methods requiring 1-2 buffers, and

Econo Buffer Selector and is the mode

of

1-

To

choose the software mode

1.

_j'o0

@e

2.

tJ~

0 0

0

3.

of

operation:

Stop the pump.

Ensure that the "Gradient Former" light the "gradient former " button

light off. While depressing the Diverter Valve Key, press and

hold the Gradient Former Key on the front panel the Econo System Controller for approximately 3

seconds. The indicator lights on the front panel Econo play

Standard Mode or "Enh" for Enhanced Mode.

Pump will flash and the four-digit LED dis-

on

the

pump

will

if

necessary to tum the

display

either

is

off. Press

"Std"

of

the

for

24

4. To choose a mode, press the Arrow Keys to choose between

"Std" and "Enh".

5.

~

To accept the selected mode, ensure that it is dis-

played on the four-digit LED display, and press the Run/Stop Key on the front panel

~

The system will remain in the mode selected until a

different mode is selected (even after power-down.)

The Standard and Enhanced modes have separate

memories. Methods programmed in one mode do not affect the other.

7.3 lsocratic (Single-buffer) Methods

To

run the system in a simple isocratic mode (use

only the buffer A inlet, use the following procedure.

1.

Ensure that both the Model EP-1 Econo Pump and Model EM-I Econo UV Monitor are plugged into a grounded power source. The pump will power up in

UV

the Stop mode, and the Allow at least

15

minutes warm-up time for the UV

monitor with the lamp

lamp.

of

the Econo Pump.

of

only one buffer) using

on.

o 0.8

0 1.6

03.2

0 CAL

IWCIE (,WPS)

Q

z..o

c

1.0

c o .

.s

"'0·:1~

Cl

0.1

~

"'

.os

"'

...

5\

Q

.01

l.Y..J

01

0

2.

Configure the system

in

the "Std" software mode.

Refer to Section 7.2 for instructions.

3.

Set the desired

UV sensitivity using the arrow keys

on the UV monitor front panel. See the UV monitor

manual for details.

4. When using the system in an isocratic fashion, make

sure that solvent line A

is

being used, and is properly

connected to the proportioning valve. Cap the unused

"B"

port on the proportioning valve.

5.

Calibrate the pump by either choosing one

pre-selected tubing sizes,

or

user-calibrating any

of

three

tubing. See the pump manual for detailed calibration procedures.

6.

With the tubing in place, set the desired pump speed

in ml/min using the arrow keys.

25

To

initiate flow, press the Run/Stop key.

To

stop

flow, press once more. Flow rate will be displayed

mllmin. Change the flow to the desired speed

in

while the pump is running by pressing the arrow

keys.

tiB

• 0

0

8

Eluant can be directed

using the two-way diverter valve key located on the system controller, plumbed.

Simply press the key

to

suitable collection devices

if

the diverter valve has been

to

light the indicator

light below the figure corresponding to either waste

of

or collect. The default position

the valve is in the

waste position.

Tip: The system can be programmed for automatic shut-off

cratic run. This can be done by programming a total run volume

of

(V pump will shut off once the total run volume has been delivered. For detail, see

Tip in the following section.

Time Based Collection with the Model 211 0 Fraction Collector

To

enable the Model 2110 Fraction Collector:

1.

Connect the fraction collector to the system con-

or

troller System Cable

Model EP-1 Econo Pump using Econo

1.

the iso-

).

The

1

2.

Press the Fraction Collector key on the Model EP-1

Econo Pump front panel to enter the fraction collector edit mode. In the edit mode, only the flashing

keys are active. Note that the Fraction indicator lights are flashing, prompting entry tion size. Enter the fraction size

using the Arrow keys. entered, press the Fraction Collector key once more to confirm the fraction size.

3. Enter a void volume the Arrow keys. See the Model EP-1 Econo Pump manual for details on the Void Volume (V tion. Press the Fraction Collector key to confirm this

value and enter the Total Run Volume

If

a void volume is not desired, set V o to zero.

26

(V

After

)

in 1

0

in

Size and

0.1

ml increments

of

ml

frac-

the fraction size is

ml

increments using

)

func-

0

(V

edit mode.

)

1

4.

5.

Enter the Total Run Volume (Vt) in 1 ml increments

using the Arrow keys. the fraction collection scheme is disabled. data entry, start at Step 2 above. All values previously entered remain in the memory until other values are entered to replace them.

After entering a

Collection key to confirm the value. The flashing LED

multi-display shows the estimated number

tions to be collected.

If

zero

is

entered and confirmed,

To

resume

Vt

value, press the flashing Fraction

of

frac-

6.

7. For options while running a method, see Section 7.9.

8.

Lf9

le.BFHI

Press the flashing Fraction Collection key once more

to enable the fraction collector.

played in ml/min.

press the

it was not already running. (The fraction collector can be programmed with the pump running

After the fraction collection program is initiated, the LED display shows the progression collection scheme in minutes, to the nearest tenth minute.

Note: Insure that sample has been injected into the

column before starting a program.

To

abort the fraction collection scheme at any time during the program, press the Run/Stop key twice. The pump will stop, and the LED display will read

OFF.

Program Run key after starting the pump, if

To

actuate the fraction collector,

Pump output is dis-

or

stopped.)

of

the fraction

of

a

9.

TIP: The fraction collection control feature

can be used to tum

Simply enter zero for fraction size. After

if

the pump, The pump will stop automatically when the total run volume has been deliv-

ered.

not already running, then press the flashing Program Run

To

hold a collection program that has begun, press the lit Program Run key once. program, press once again.

while the program is on hold will abort the fraction

collection scheme.

of

off

the pump even

if

a fraction collector is not connected.

confirming the V t value entered, start

To

resume the collection

Pressing the Run/Stop key

the Model EP-1 Econo Pump

key.

27

7.4 Binary Gradient Formation

The Econo System gradient former functions with the Model EP-1 Econo Pump and the integral proportioning valve and mixer to form a binary gradient consisting

gradient on the front panel is by no means the only way to

of

up to five time segments. Note: the graphic display

of

the system controller is provided as a guide,

format

a five segment gradient.

of

and

a

o 0.8

0 1.6

03.2

0 CAL

01

0

1.

The pump must be calibrated in order to form gra-

of

dients. See Section 5.2

the Model EP-1 Econo

Pump manual for details.

2.

Configure the system in the "Std" software mode, if

not already done. Refer to Section 7.2 for instruc-

tions.

3. The gradient profile can be constructed in the following way:

Inflection

Point Elapsed

I 0

2 3 4 5

6

Time

T2 T3 T4 T5 T6

%B

Bl B2 B3 B4 B5 B6

4.

5.

Press the gradient key once. The

min

light on the

pump and the pump display will flash "0", to indicate time=O. Press the gradient key once more. The

% light on the pump will flash, along with the light

above the first gradient point on the gradient dis-

0,

or

play. The pump display reads

ly entered value

for initial % B

for%

(B

of

buffer

1)

using the Arrow keys. Confirm

the most recent-

B.

Enter a new value

entry by pressing the Gradient Former key. The gradient point indicator will then advance to the

next gradient inflection point. Note the flashing

"min" indicator light on the pump. Enter the time

desired for the first gradient step (T2), or leave the

previously entered value unchanged. Confirm this

value by pressing the Gradient Former key.

28

6.

The flashing

% LED indicator light prompts entry

of

a % B value for this inflection point (B2). Enter the

new value, and press the Gradient Former key to

confirm this entry and advance to the next gradient

inflection point.

7. Follow steps 4-5 for the remaining gradient points.

8.

9.

10.

If

using fewer than the six inflection points allowed,

scroll through the time and

% points for the remain-

ing gradient inflection points by pressing the Gradient Former key, without changing the values showing.

Note: The stop indicator light on the pump will flash

at every step during programming to indicate that this key may be pressed at any time to exit the edit mode. Upon exit, any changes made to the method

are automatically saved.

After the last % B value has been selected (or left

unchanged) and confirmed, the gradient former will be enabled, as indicated by the lit Gradient Former

key.

If

a fraction collection program has been entered

previously (note light under the fraction collector

of

icon on pump front panel), the number

fractions

to be collected will be re-estimated and displayed

at this time.

Press the flashing Gradient Former key a final time. The LED will be lit steadily, indicating a gradient is programmed and enabled.

At this point, the gradient former

formation.

If

the gradient is not desired, press the

is

set

up

for gradient

Gradient Former key one more time to tum off the gradient former indicator light.

29

To

begin the gradient scheme, press the Run/Stop

If

key to get the pump running. condition programmed is not

you will hear the proportioning valve clicking as the system operates at initial gradient conditions. start the program, press the flashing Program Run key

on

the pump. The gradient program will begin,

with the LED display showing minutes progressed.

To

observe the other parameters scroll through with the Arrow keys as described Section 7.9.

Note: Insure that sample has been injected into the column before starting a program.

the initial gradient

100% A or 100% B,

To

of

the program,

~n

13. For options while running a method, see

Binary Gradient Time-Based with

the

Model

The system should be configured in the "Std" mode,

See Section 7.2 for instructions.

0

0.8

orr

0

1.6

03.2

0

CAL

oBJ

~

0 v

o

0

v~

0

211

0 Fraction Collector

1.

2.

3.

Calibrate the Model EP-1 Econo Pump as described

in the pump manual.

Enter a fraction collection program as described in

Section 7.3. See Appendix A for connection fraction collectors.

Program a gradient as described previously.

Collection

Section 7.9.

if

not already done.

of

other

4.

~r7onlo

lQJ~

5.

Start the pump to begin the gradient at initial conditions(% collection by pressing the flashing

To

discontinue collection only, press the Run/Stop key twice, and turn off the fraction collector by pressing the Fraction Collector once more to start the pump, and the Program Run key to start the gradient at initial conditions.

30

Bl).

Start the binary gradient time-based

Program Run

key.

Then press Run/Stop

key.

6.

Note: After the gradient former program has been entered, the total vol-

(Vt)

ume

and estimated number ed based on the total gradient time and flow rate. Fraction Collection program to view the adjusted

For options while running a method, see Section 7.9.

of

fractions to be collected will be adjust-

Scroll through the

Vt

and fraction num-

ber values.

7.5 Peak Detection and Collection

The peak detection and separation functions

of

the Econo System allow

the user to discriminate among various chromatographic peaks using either

or

an absorbance threshold programmed into the system,

by setting time

windows, where collection occurs within selected time windows. Both meth-

of

ods

peak collection operate using the Model SV-3 diverter valve, which

functions to divert the eluant stream from a waste line to a collection line. This

collection line can be directed into a fraction collector for more precise peak

breakdown, gram, gradient former, tion function. "Std"

Fig. 7.1. Peak detection and collection features.

or

into any suitable collection device. A fraction collection pro-

or

auto shutoff must be used with the peak collec-

Peak detection and collection features may be used in either

"Enh" software modes.

PEAK COLLECTOR

Peak Collection by Threshold

Peak collection on the basis

of

sion

peaks to a fraction collector, while directing non-peak eluant to a

of

threshold detection allows for the diver-

waste container or other collection vessel. This a peak threshold value in percent full scale

1.

To operate the threshold detection function

UV absorbance.

Econo System, a fraction collection, Vt timer, gradient formation program must be enabled. See

Section

7.3 and 7.4 for details.

31

is

accomplished by selecting

of

the

or

~

A:.

)C:

:;~:

..

_;

A

• '0

0

:'0)-

..

,~JU

NO

0

-:~

2.

Press the Peak Collector key until the indicator light

below the threshold icon is flashing. The Pump LED

will read %, prompting entry

for percentage Arrow keys to scroll up threshold value. For reference purposes, each green

light on the absorbance display Econo UV Monitor equals 10% lights

lit=

50%

of

full-scale absorbance. Use the

or

of

full scale).

down

of

a threshold value

to

select the desired

of

the Model EM-I

of

full scale (five

:A:.

• '0

0

~~

@ ,..

BZJI::·8.3BI..:~

0

08

• 0

0

t!B

• 0

0

o

BJ

~

0

Vo

•

0

v,

0

3. After entry

A

Collection key once more to enable threshold detection. The light below the threshold icon will be lit.

4. To

Run/Stop key, followed by the flashing Run key. key twice within 1 second.

5.

•O

>0

6.

When

is in the Waste position, with the light under the

is reached, the diverter valve will switch to Collect,

and a mark

recorder tracing.

If

start

waste icon lit. The pump LED display reads minutes progressed. When the selected threshold value

a void volume has been programmed, the valve will remain in the waste position for the duration the

void. absorbance is above the selected threshold value, the valve will revert to collect, then back to waste after the absorbance drops back below the set threshold value. With each valve change, a mark will be

superimposed on the recorder tracing.

of

this threshold value, press the Peak

the

peak

To

Stop the program, press the Run/Stop

Peak Collection is enabled, the diverter valve

will

At

be

the

detection

end

program,

superimposed

of

this time period,

press

Program

on the

the

chart

of

if

the

0

LI

0 0

0

B

7.

The Diverter Valve key allows the user to toggle

back and forth between waste and collect. The Peak Collection function will override the manual place-

ment valve has been manually placed into the collect position prior to a peak, when that peak valve will stay in the collect position, then switch

of

the diverter valve. That is,

if

the diverter

is

detected, the

32

to waste on the tail end

value.

of

the peak when the absorbance drops below the set

AA

• '0

8

0

0 0 9

l1 B .

0 0

0

11.

~~0

LQJ~

To change the threshold value during a run, press

·

the

Peak Collection key to enter the edit mode for

by

threshold detection, indicated under the threshold icon. indicator lights will now

of

a threshold value in % full-scale absorbance. To confirm this value, press the once more. The changing the threshold setting.

If

the

new absorbance value currently seen tor, the diverter valve will switch from collect to waste, and vice versa

current value.

To terminate the method, press the Run/Stop key

twice within 1 second. For other options while running a method, see Section 7. 9.

pump

threshold

The

show%,

will continue

value

if

the new value is less than the

a flashing light

pump

Peak

is

LED

prompting entry

Collection key

to

greater by

the

run

than

UV

display

when

the

moni-

Peak

material known to elute at a specific time. The user can select the starting and ending times for three peaks, as indicated beneath the three representative peaks (see Figure 7.1). To operate the time windows method dient formation feature must be enabled.

Collection by

Peak collection on the basis

AA

0

'i'

0

~0

(•

o o o o

oD

Time

of

peak collection, the fraction collection, V1 timer,

1.

2.

3.

To enter the edit mode for time windows collection,

press the

until the light below the time window icon flashes.

Enter a time value for the starting point for the first

peak in

on the pump. To

advance to that time window end point, press the Time Windows key.

Windows

of

time windows will simplify collection

by

the six indicator lights located

Peak Collection key, and continue pressing

0.1 min increments using the Arrow keys

confirm

the

time

window

starting

or

point

of

gra-

and

33

/l-6-6

(•

• o o o

oO

4.

Enter the time window endpoint value in

increments using the Arrow keys. Advance to the next time window starting point by pressing the Time Windows key.

0.1

min

AA

0

..

0

t!B

• 0

0

~

0

5.

6.

7.

if

or

Peak

the

V o is

Repeat steps 2-4 for the remaining two windows,

confirm entry time window collection feature by pressing the

Collector key. The light beneath the Time Windows icon will be lit solid, indicating windows feature.

To

start Run/Stop key followed by the flashing Program Run key.

The diverter valve will reside in the waste position

until a time window is reached. At that point, the valve will switch over to the collect position. The valve can be manually switched using the Diverter Valve key. The position determined by the programmed time window feature will override any manual positioning

superimposed over a time window, the diverter valve will be in the waste position. Fraction Collector key.

the

of

that time window and enable the

Time

activation

Windows

of

the valve. However,

program,

To

cancel V

of

press

o'

the time

press the

AA

0 'i'

8.

0

~~o

9.

Changes to the window's start and end points can

be made by stopping the program, scrolling through

the time windows with the Time Windows key, and entering and confirming the new values as described

Steps 2-4. Pressing the Peak Selector key without

in

changing any

viously entered values and invoke Time Windows.

To

terminate the Time Windows program, press the Run/Stop options while running a method, see Section 7.9.

of

the time points will confirm all pre-

key

twice within 1 second.

For

other

34

The Time Windows detection function can be run

in tandem with the gradient former to optimize sample collection. Simply program the gradient former

as described above prior to invoking the time win-

dows collection function.

7.6 Programming Multi-Step Methods (Using the Econo Buffer

Note: mode

The

Econo System must be configured

for

automated valve control using the Econo Buffer Selector.

Selector)

in

the Enhanced software

you are not familiar with programming the Standard Econo System,

7.1-

7.5

of

please review Sections

10 13 23 33 43

55 55 55

this manual before proceeding.

B

c

D

E

A A A

End

If

where:

Table 7.5. A Typical Method Table for a Multi-Step Method. The method outlined

consists of 4 buffers, 1 sample, and 6 steps (7 inflection points).

Programming methods in the Enhanced software mode involves a method table with a maximum Table 5.4 and Table 5.4.b.). Each inflection point is defined by an elapsed (cumulative) time and a buffer/valve position. Any buffer/valve position may be selected at each inflection point.

A multi-step method is a method which consists from one buffer to another. The Model

trol up to 5 different buffers (one

can consist

of

up to 8 steps (created by 9 inflection points).

A = Loading Buffer B

=Sample C = Elution Buffer D = Elution Buffer #2

E = Regeneration Buffer

of

9 inflection points (equivalent to 8 steps) (See

of

35

#1

of

a sequence

EV-1

Econo Buffer Selector can con-

which could be a sample) and a method

of

steps

o

0.8

0 1.6

03.2

0

CAL

or

0

1.

The Econo Pump must be calibrated: Calibrate the

Model EP-1 Econo Pump manual.

EP-1 Econo Pump as described in the Model

2.

3.

Press the Run/Stop key to stop the pump.

Press the Gradient Former key once. The pump dis-

play will flash digit display on the Buffer Selector will flash cating the first inflection point in the method.

0,

indicating

time=

0. The single

1,

indi-

Note: The Stop indicator light on the pump will flash at every step dur-

to

ing programming

exit the edit mode. Upon exit, any changes made to the method are automatically saved.

4.

indicate that this key may be pressed at any time to

Press the Gradient Former key again. The single-

digit display on the Buffer Selector will still be flash-

1 for inflection

ing pump appear on the pump display. (L is used for binary

gradient steps and is explained in Section 7.7.)

will flash, and a letter (A,b,C,d,E,L) will

point

#1. The % light on the

8.

Use the

Confirm the entry by pressing the Gradient Former key.

The min indicator light on the pump will flash and

the single-digit display on the Buffer Selector will

flash 2, indicating inflection point #2. Using the Arrow keys, enter the elapsed time desired for the second inflection point. Gradient Former key.

The

Arrow

% light on the

keys

to

select a valve

Confirm entry by pressing the

pump

will flash, and a letter

position.

(A,b,C,d,E,L) will appear on the pump display. Use

the Arrow keys to select a new valve position. Press the Gradient Former key to confirm the entry and advance to the next inflection point.

Repeat steps 6-7 for the remaining inflection points.

36

9.

If

using points, scroll through the time and % points remaining method inflection points

Gradient Former key, without changing the values showing, Run/Stop key.

*If viously entered method are not automatically erased. Ensure gramming steps will not interfere with the current method.

After the last elapsed time point has

and confirmed (inflection point the

"End". Press the Gradient Former key once more

to

enable the method. The Gradient

tor

If

a entered (the Fraction Collector indicator light pump of

will "F confirm the gradient program.

indicator light should

fewer

the edit mode is exited, data points from any pre-

that

Buffer

light should be lit.

fraction

front panel will

fractions to

be

re-estimated and the pump display will flash

###".Press

than

the

maximum

or,

exit

the

edit

the

data

points

Selector), the

collection

be

collected

the flashing Gradient

program

be

lit), the estimated

be

lit.

of 9 inflection

by

pressing the

mode

pump

by

pressing

in

the

remaining

been

#9

as displayed

display will flash

Former

was

previously

in

the method entered

Former

The

Gradient Former

for

the

pro-

entered

by

indica-

on

the

number

key to

17-nl~

12.

~lQJ

13.

14.

To begin the method, press the Run/Stop key to start

the

pump

start

To Program Run key on the pump.

The pump display will show a flashing

number repeat. to 999. The default for n is

If

a manual sample injection is method, ensure that sample has been injected into the column before starting the program.

Press the Program Run key to confirm the entry

"n".

appear on the chart paper.

running at the initial gradient condition.

the

method

of

times the method should automatically

Use the Arrow keys to enter a value from 1

The

method will begin and an event

program,

1.

press

the

"n". "n" is the

necessary

flashing

for

mark

the

of

will

37

7.7 Programming Step Methods Which Include

a Binary Gradient (Using the Econo Buffer Selector)

A multi-step method which includes a binary gradient is one common types could include:

• column equilibration with a buffer (buffer A)

• automatic injection

• a linear gradient (from 0% buffer B to 100% buffer B)

• column washing with a regeneration buffer (buffer D)

• column washing with a buffer appropriate for column storage (buffer E)

When programming a method, any buffer/valve position may be selected at each inflection point. The relationship between valve positions and buffers is:

Valve Position:

Buffer:

of

chromatography methods used. For example, such a method

of

a sample (buffer C, >5 ml volume)

A

b

L

Alb

c c

of

the most

d

E

(gradient)

The L (for

inflection point.

Standard software mode.

proportioning and mixing valves on the Econo System Controller before connecting to the Valve which a linear binary gradient may be formed. L can be programmed at any step in the Enhanced method table, and it can be used only once per method

(See Table 7.6.).

"linear") is one choice for the buffer/valve position at each

Lis

identical to the 5-step method that is programmed

Because buffers A and B are plumbed through the

Pod

AlB

inlet port,

Lis

the only valve position in

in

the

38

~ection

~<

' . . .

···Point

·:,:

·.

:··

··)

...

:Elapsed time

.'

..

,;

(Dlin)

1

....

0

2 5

3

4

.

5 6 7

8

9

where:

Table 7.6. A

Binary sample, 7 steps (8 inflection points), and a binary linear gradient

10 15 45 50 60 70 70

Gradient.

i:

=<

ValvefBuffer

>'

,:

,

..

A

c

A

L

A

D

E

End

A = Loading Buffer B = Elution Buffer C=Sample D = Regeneration Buffer E =

Column Storage Buffer

Typical

Method

The method tables outline a method which consists of 4 buffers, 1

Table

for a Multi-Step

~ection

<P~inf . (~n)"

••···.

•.

4:

.·

;6

...•...

Method

STEP

Elapsed time · %B

"L:'

· .

15

30 35 35

45 100

··.

45

Which

(Step L).

Includes

0

50 50

100

0

a

7.8 Programming Automated Sample Injection

The Model

of

tion

samples.

However,

1 mllmin. This is not recommended since sample loss may occur due to tub-

ing volumes.

Samples smaller than 1 ml should be diluted to 1 ml, ly using the Model MV-6 Sample Injection Valve. Always keep tubing connections as short as possible to avoid excessive loss

For the automated injection method):

1.

Calculate the amount

tem based on the sample volume and method's flow rate. This calculat-

ed time will be the duration method. For example, 50 minutes duration for that step.

EV-1

is

is feasible to load samples as small as 1 ml using a flow rate

Econo Buffer Selector can be used to automate injec-

The

recommended

of

time necessary to pull the sample into the sys-

50

ml

of

minimum sample

of

sample.

volume

or

loaded manual-

samples (injection programmed within a

of

the injection step, programmed in the

sample pumped at 1 mllmin will require

is 5 mi.

of

39

2. Prime the tubing connecting the Model SV-5 Valve Pod and the sample reservoir with either the appropriate with sample itself. Plumb the sample reservoir to either valve port C, D,

E.

Minimize the tubing length between the valve pod and the sample

or

as

reservoir amount used in the calculations in step #1.

3.

For very large volume samples and especially when automatically repeating injections by self-looping methods, ensure that the actual volume

sample plumbed to the system matches the calculated volume

to be injected by the method program.

7.9

Options While Running A Method

The following options are available while running a method in either

the Standard or Enhanced software mode.

1.

While a method is running, the following information can be displayed by pressing the Arrow keys on the pump. The units for the data displayed are indicated by the indicator lights to the right The following is an example:

much as possible.

of

sample delivered to the column may differ from the amount

If

"binding" buffer for the column,

excessive lengths

of

tubing are used, the

of

the display.

or

of

sample

Information

Volume

Elapsed time

Flow rate

Inflection point and buffer

%B

Repeat#

Display

" 30"

" 123"

10"

"

"7-A"

"b 50"

"n

3"

Indicator Light

ml

min

ml/min

%

Meaning

30 ml been delivered in the current run.

Time elapsed in current run is

123 minutes.

The flow rate is

10 ml/min.

Method on step #7, valve port

selected. System currently

forming 50%B

AlB

The third cycle the repeating method is being run.

of

solution has

A is

of

binary gradient

of

40

2. After method initiation:

• To hold the method with pump stopped, press the Program

"Hold". To continue, press the Program Run key

once more, or to abort, press the Run/Stop key.

• To hold the method with pump running (holding the current valve port ent former constant), press the Gradient Former key. The Gradient Former indicator light on the system

controller and the pump display will flash. tinue the method, press the Gradient Former key once more.

The fraction advance and UV threshold level detector (if enabled) to be collected on a volume basis. is enabled, fractions will continue to be collected only if the system is held.

Run key. The pump display will show

or

proportional rate

are

still active. Fractions will continue

is

collecting at

the

of

the gradi-

To

If

Time Windows

time the method

con-

While holding the method with the pump running,

run-time parameters can still be viewed on the pump display by pressing the Arrow keys. To stop the

pump, but continue holding the method, press the Program Run key. Press the Program Run key to start the pump again.

To resume the method, press the Gradient Former

key.

3.

To

terminate the method at any time (e.g. stop the pump and the method):

Press the Run/Stop key twice within 1 second. The

pump display will show

"OfT".

4. To change a program, exit the method (see step 3) and re-enter the edit mode:

Press the Gradient Former key

Scroll

through

the method to the

to

enter the edit mode.

point

where a change is desired. Make desired changes and exit by pressing the Run/Stop key.

41

5. At each valve change during a method, an event mark will be superimposed on the chromatogram trace.

switched off. Standard and Enhanced modes have separate memories, so methods entered in one mode do not affect the other.

10 hours.

Section Storage

operation. Following are general procedures for maintenance System and components.

ation, spills and splashes may result in precipitates forming on the component cases, fraction collector carousel, and rack. water to wash down the rack and outer cases ments are resistant to spills. Liquid draining off the cases monitor will run onto the system controller, where it will drain out the back

of also has a drain out the back

Once entered, a method is retained in memory even

If

the system is turned off, methods are retained in memory for at least

if

the

power

is

8

and

The Econo System requires very little maintenance to assure reliable

Econo System component cases. carousel. and rack: During normal oper-

the case through the drainage tube. The Model 2110 fraction collector

Maintenance

of

the unit.

of

the Econo

Use a squirt bottle and soapy of

the instruments. The instru-

of

the pump and UV

Model EP-1 Econo Pump: Check tubing regularly for signs

If

and wear. the pump's electronics. When not in use, tubing should be removed from the pump to prevent deformation the tubing by unhooking one end prevent the formation brane key panel, promptly remove any spills.

Model Monitor: Clean the outside surfaces Monitor,

and 254 nm filters in the UV optics unit should be cleaned only when nec-

essary with a dry lens tissue and replaced in the optics module.

dilute ethanol or isopropanol solution will assist in the removal nants. When the disconnect and clean out dissolved salts or protein using a syringe filled with distilled water. do not allow the flow cell (10-25%)

any exist, replace the tubing to help prevent leakage

of

the tubing. Remove the platen and relax

of

the tubing from the tubing bracket.

of

precipitates around the pump head and on the mem-

EM-I

UV optics module, and flow cell as described above. The 280 nm

of

Econo UV

UV optics module or gradient monitor flow cell is not in use,

To

prevent the formation

ethanol

or

isopropanol to prevent microbial growth.

Monitor

to

dry out. Store the flow cell in a dilute solution

and Model EG-1 Econo Gradient

of

the Econo UV Monitor, Econo Gradient

of

salt crystals and solute deposits,

of

cracking

of

fluids into

Use

of

contami-

To

of

a

42

Model 2110 Fraction Collector: Clean the instrument case and carousel as described above. The drop sensor's optical window should be cleaned periodically with a cotton swab moistened with water.

Proportioning, mixing, and diverter valves: These valves require essentially no maintenance other than to flush salt solutions out after use with a syringe filled with distilled water.

If

operation. above, with soapy water.

salt solutions are spilled onto the valves, rinse, as described

Storing the valves dry will not affect their

Model

SV-5 Valve Pod: Solutions containing dissolved solids should

never be allowed to dry inside the valve pod.

flushed out periodically with deionized water.

For long-term storage, store the valve pod by injecting a dilute solution ( 15-25%) Use the end caps provided to seal all ports.

system tubing with distilled water. Power down the system by turning off

the power switches on the bottom panel

panels

Section

of

ethanol or isopropanol into the ports

Prior to long-term storage

of

all other components.

9

Troubleshooting

This section contains a brief troubleshooting guide to help you quickly

locate the cause the Econo System and suggest possible remedies. In all cases, do not hesitate to refer to the pertinent component instructions for more detailed information. Check the following before calling Bio-Rad Technical

The valve ports should be

to

prevent microbial growth.

of

the Econo System, flush out all valves and

of

the pump and UV monitor, and rear

of

any problems you might encounter in the operation

Service:

of

•

Check the connection Pump, Model EM and the Model 1325 Econo Recorder, plied to these components.

• Check the connection and

UV monitor to the system controller.

•

If

you are using a non Econo System UV monitor, fraction collector, chart recorder, nected with the proper cable to the Econo

•

Check the tubing routed throughout the system for indications ing solvent. Insure that all valves are operating properly and are free blockages.

or

of

the power cables to the Model EP-1 Econo

-1

Econo UV Monitor, Model 2110 Fraction Collector,

if

used. Ensure that power is sup-

of

the ribbon cables between the base

other component, insure that each instrument is con-

System.

43

of

the pump

of

leak-

of

• Check for air bubbles within the system lines. Disconnect column and purge to remove. Isolate and seal possible to reduce the possibility

of

air

bubble formation.

air inlets. Use degassed solvents

Problem: Action:

Faulty numeric

1.

2.

3. Unplug power cord to pump. Simultaneously press both the

4. Call Bio-Rad Technical Service.

1.

2. Ensure that baseline has not drifted off the bottom

3. Full scale

Insure that the Econo Check

Tum

downward pump front panel, while plugging the until display clears.

No Be

paper.

the ting is set at 1 V full scale.

Pump

power connection to the pump.

pump

peaks are observed

sure correct sample

of

UV

monitor. Insure that chart recorder input range set-

pump

40-pin

and

off, then

arrow

graph set too high. Decrease AUFS setting

LED

connector

system

back

and

the

on

was

display upon

between the Model EP-1

controller

on

again.

tubing

the chromatogram.

injected properly.

calibration key

power

is properly affixed.

pump

back

up.

on

in. Hold

of

the

chart

on

Problem: Action:

4.

5.

1.

2.

1.

2.

Detector malfunction. Use detector's event fy proper operation.

Lamp disconnected UV

Peaks are clipped Graph scale is too low. Readjust the UV

monitor. Detector overrange.

or less injection volume. Retention times vary between injections

Column clogged. column.

Flow rate is not reproducible. Check tubing for signs wear. adjustment. Check for restrictions on inlet side tioning valve, mixer, and all tubing. ters on inlet side.

Replace

or

dead.

or

Use a lower

Remove

damaged

UV

lamp indicator will flash.

squared off.

concentration

obstruction

or

worn

tubing.

Do

AUFS setting

mark

of

same material.

or

replace/repour

Check

not use in-line fil-

to veri-

of

sample

of

propor-

on

platen

the

of

44

Appendix

A

Interfacing

For the Econo System Controller to function, it must be connected to the Model EP-1 Econo Pump. For automated valve control, the Model Econo Buffer Selector must be connected to the Econo System Controller. Aside from this requirement, non Econo monitors, fraction collectors, chart recorders, RI monitors, and conductivity monitors can be connected to the system through output sockets on the rear panel

three 8-pin mini-DIN connectors, an 11-pin access a variety signals, you must insure that the circuit external to the Econo does not draw more than tion

of

the Econo System controller signal outputs.

1. Fraction Collector Output

~

and

the Econo System controller (see Figure 3.2). In addition to the

v

Signals

of

signals. All output signals are

15

milliamperes

Pinout Signal Type

*

2 No Contact 3 No Contact 4 5

6

7 8 Fraction Marks to

EV-1

System components such as UV

I/0

connector is available to

TIL

compatible.

of

current. Following is a descrip-

Shield

1 Fraction Advance

(low going output)

No Contact Fraction Advance

(high going output)

No Contact

Ground

Recorder (low going input)

To

use these

System controller

A fraction collector can be connected to this socket using an 8-pin mini-

DIN to bare wire cable (Econo

to the bare wires on Econo above.

2.

Output to Diverter Valve

This 8-pin mini-DIN socket is for connection

Valve only.

System Cable 7). Connect the fraction collector

System Cable 7 to access the functions listed

of

the Model

45

SV-3

Diverter

3. Chart Recorder Output

Pinout

*

1

2

3

4

5 6

7

8

A chart recorder can be connected to this socket using Econo

Cable 7 as described in the Fraction Collector Output section.

Signal Type

Shield Start/Enable

No Contact Mark A (output) No Contact No Contact Pen (output) Ground

Mark B (output)

(output)

System

4. Eleven Pin 1/0 Connector

The 11-pin connector described below is used for connection Econo Buffer connection

UV monitors, and conductivity monitors. The

tors, are readily available for connection to bare leads from the instruments.

Following is a description

Selector to the System Controller. It may also be used for the

of

other manufacturer's instruments including fraction collec-

11

screw-terminal pinouts

of

the pinouts.

of

the

10mV------------~

DIGITAL

GND

---

........

FCADVANCED=)l

FCADV(+)~

FCADV(·).,

I

1 2 3 4

Pinout

1

2

3

4 5

6

Signal Type

Fraction Collector Advance (low going output)

Fraction Collector Advance

(high going output) Fraction Advanced (signal input) Digital Ground

10 mV Input

100 mV Input

Pinout

7

8

9

10* 11*

Signal Type

1 V Input

Analog Ground Shield Buffer Selector Buffer Selector

46

In the Standard software mode pin

PTFE

*

to power a column bypass valve(s). In the Enhanced mode, pin

EV-1

Model

Econo Buffer Selector and pin 4

10

and pin

11

are the output of the driver circuitry which can be used

is

the common pin.

11

serves as the data out pin to the

To connect a non Econo System

output, the leads from the monitor can input and analog ground terminals. used, a chart recorder must troller. Diverter valve and peak selection marks will not appear on a chart recorder tracing

To access the fraction advance signal for a non Econo System fraction collector, the leads from the collector can and digital ground screw terminals. The signal type (high or low) depends upon the fraction collector being used. Connecting the collector's fraction advanced signal lead to pin recorder.

Appendix System

Specifications

Following are specifications as they relate to the Econo System con-

troller. For specifications related to individual Econo System components, refer

to the specific manuals.

B

UV

monitor which has a 1 volt signal

be

connected directly to the 1 volt

If

a non Econo System UV monitor

be

connected to it rather than to the system con-

if

a non Econo System monitor is connected.

be

connected to the fraction advance

# 3 will allow fraction advance marks to

is

be

sent to a chart

being

Gradient former

Gradient accuracy

Gradient

Mixing valve volume Peak detection

Diverter valve

Sample

Buffer reservoir

linearity

Threshold

Time windows

injector valve

5-time segment (6-point) gradient capability 1-9999 min.

0-100 % buffer partitioning via a 3-way solenoid proportioning valve

%of

Within 1 5% and 95%

3%

full scale

475

j..tl

Threshold or time windows

0-99 % of selected

Maximum 3 time windows, minutes

Three-way solenoid valve

vent resistant housing

Six-way valve with female luer fittings (6). Dead volume=

21.8 (for two 1 L bottles, incl. ice)

60j..tl,

X 15.2 X 15.0 em

full scale between

"B"

AUFS range

in

sol-

W'

x 28 to

including luer fittings.

(W

X 0 X H)

47

Component

(signal outputs)

Signals

Operating Dimensions

Weight

Material

temperature

of

connectors

construction

Econo pump:

Econo UV monitor: 34-pin ribbon cable

Fraction collector: 8-pin mini-DIN Diverter valve: 8-pin mini-DIN

Chart recorder: 8-pin mini-DIN

Auxiliary: 11-pin Combecon connector

TIL

logic

4-40

oc

32.3 X 40.6 X 16.0

2.95 kg

Noryl

40-pin ribbon cable

em

(W X D X H)

Appendix Ordering

Model Econo

Catalog Number

731-8140

731-8142

731-8145

Model Econo

731-8160

731-8162

EP-1

Pump

EM-1

UV Monitor

C

Information

Product

Model

ing set and starter fittings kit

Model set and starter fittings kit

Model

Model starter fittings kit

Description

EP-1

Econo

EP-1

Econo

EP-1

Pumphead

EM-1

Econo

Pump, 110 V (USA power cord), with tub-

Pump, 220 V (no power cord), with tubing

Assembly

UV Monitor, 110 V (USA power cord), with

UV Monitor, 220 V (no power cord), with

731-8165

731-8166

731-8167

731-8168

Model

EM-1

Flow

Cell

Lamp

Filter

Optics

Assembly

Module

48

(280/254 nm)

Assembly

Model

EV-1

Econo Buffer Selector

Catalog Number

731-8171

731-8170

731-8236

731-8270

731-8271

731-8272

Model

EG-1

The Model

EV-1

Product Description

Econo Buffer

adaptor (catalog no. 731-8270), and valve pod (catalog no.

731-8236)

Econo Buffer Selector, Model EV-1, requires power adaptor,

includes valve pod (catalog

Econo Valve Pod, Model SV-5, 1 Power Adaptor, 100-120V, for USA, Canada, Japan, Mexico,

Taiwan, Latin America

Power Adaptor, other countries not

Power Adaptor, 220-240V, for UK, Australia, New Zealand

Econo Gradient Monitor

Econo Buffer Selector requires a power adaptor.

Selector, Model EV-1, with 1 00-120V power

no.

731-8236)

220-240

V,

for Europe (Except the UK) and

specifically listed

The Model EG-1 Econo Gradient Monitor requires a power adaptor.

731-8151

731-8150

731-8155

731-8270

731-8271

731-8272

Econo Gradient Monitor, Model EG-1, with USA power adap-

tor (catalog no.731-8270) and flow cell (catalog no. 731-8155)

Econo Gradient Monitor, Model EG-1, without power adaptor,

includes flow cell (catalog no.731-8155)

Flow Cell, Model EG-1, 1 cell Power Adaptor, 1 00-120V, for USA, Canada, Japan, Mexico,

Taiwan, Latin America Power Adaptor,

other countries not

Power Adaptor, 220-240V, for UK, Australia, New Zealand

220-240V, for Europe (Except the UK) and

specifically listed

Model 1325 and Model 1327

(Dual Pen) Econo Recorders

Note: Both recorders require a power adaptor and accept the same type of pen and chart paper.

731-8190

Model1325

110 V power adaptor, 2 rolls paper, 1 fiber tip pen (red), spare

fuse, 1 set banana cables.

Econo Recorder, 110

V,

single pen. Includes

49

Catalog

Number

731-8192

Product Description Model1325

tor.

Includes 2 rolls paper, 1 fiber tip pen (red), spare fuse, 1 set

banana

Econo Recorder, single pen, without power adap-

cables.

731-8250

731-8252

731-8194

731-8195

731-8196

731-8197

731-8198

167-0215

Model1327

power adaptor, 2

blue), spare fuse, 2 sets banana cables.

Econo Recorder, 110

rolls chart paper, 2 fiber tip pens (red and

V,

dual pen. Includes 110 V

Model 1327 Econo Recorder, dual pen, without power adap-

tor.

Includes 2 rolls chart paper, 2 fiber tip pens (red and blue),

spare fuse, 2 sets banana cables.

Power Adaptor for USA, Canada, Japan, Mexico, Taiwan, Latin America

Power Adaptor for UK, Commonwealth (Except Canada,

Australia, and New Zealand) Power Adaptor for Australia and New Zealand Power Adaptor for Europe (Except the UK) and

Other

Countries Not Specifically Listed Chart Paper,

10 em x 1300 em each

for Model 1325 and Model 1327, 2 rolls,

Disposable Fiber Tip Pens, 3 red pens, for Model 1325 and

Model 1327, lifetime approximately 1,000 m

167-0216

Disposable Fiber Tip Pens, 3 blue pens, for Model 1327 only,

lifetime approximately

1 ,000 m

Econo System Controller

731-8180 Model ES-1 Econo System Controller, with Model SV-3

Diverter Valve, MV-6 injection valve, fraction collector cable,

monitor cable, starter fittings kit, and 1.6 mm Tygon tubing, 5 m

731-8185

731-8186

Model ES-1 Proportioning Valve Model ES-1 Mixer Assembly

Complete Econo Systems

731-8101 Econo System, complete, 110/120

731-8109

Econo Pump, Model Econo System Controller, Model 2110 Fraction Collector, Model 1325 Econo Recorder, Econo System Rack,

Valve, MV-6 Injection Valve, tubing and fittings starter kits.

Econo System, same as 731-81

Fraction Collector

EM-1

Econo

V,

includes Model

UV

Monitor, Model

01

, without Model

SV-3

EP-1

ES-1

Diverter

211

0

UV

50

Catalog

Number

Product Description

731-8100

. 731-8102

731-8103

731-8104

731-8105

731-8107

731-8108

Econo System, same as 731-8101, without

11

Recorder,

Econo System, same as 731-8101, without

Recorder,

Econo System, same as 731-81

Fraction Collector and Model 1325 Econo Recorder,

Automated Econo System,

Model Model 2110 Fraction Collector, Model Monitor, Econo Buffer Selector, Model Econo System Organizer, Econo System Rack, SV-3 Oiverter Valve, MV-6 Manual Injection Valve, tubing and fittings starter kits

Automated Econo System, same as 731-8104, Automated Econo System, same as

2110

Automated Econo System, same as 731-8104, without Model

1327 Econo Recorder,

EP-1

Fraction Collector, 110/120 V

0/120 V

220/240 V

01

, without Model

complete, 1 00/120V, includes

Econo Pump, Model

Model 1327 dual pen Econo Recorder, Model

11

0/120 V

EM-1

EG-1

ES-1

Econo System Controller,

731-8104, without Model

Model1325

211

0

11

0/120 V

Econo UV Monitor,

Econo Gradient

EV-1

220/240 V

Econo

731-8106

731-8110

731-8200

731-8201

731-8206

Valves

731-8237

731-8235

Tubing

731-8210

Automated Econo System, same as

1327 Econo Recorder 220/240 V

Automated Econo System, same as

1327 Econo Recorder and Model 2110 Fraction Collector 110/120

Econo System Rack, (assembled) includes: base, 4 vertical

supports, 2 horizontal supports, 8 column clips, 1 test tube rack; extra parts

Econo System Organizer, with Econo System Organizer, without power strip

Model MV-6 6-Port Injection Valve Model SV-3 Diverter Valve

Silicone

v

includes 2 vertical and 1 horizontal support

Tubing, 0.8 mm

10,

0.8 mm wall,

731-8104, without Model

11

OV

power strip

10m

731-8211

Silicone Tubing, 1.6 mm

51

10,

0.8 mm wall,

10m

Catalog Number

731-8212

Product Description Silicone Tubing, 3.2

mm

10,

0.8 mm wall,

10m

731-8214

731-8215

731-8217

731-8218

731-8219

Tubing Kits

731-8240

731-8241

731-8242

731-8244

Tygon®Tubing, 0.8 mm Tygon Tubing, 1.6 mm

Norprene® Tubing, 0.8 Norprene Tubing, 1.6 mm Norprene Tubing, 3.2 mm

Silicone Tubing, 0.8

10,

mm

mm,

10,

17.1

0.8

10,

mm

0.8

em,

wall,

mm

20;

female luer-fittings, 4 male luer-fittings

Silicone Tubing, 1.6 mm,

17.1

em,

20;

female luer-fittings, 4 male luer-fittings

Silicone Tubing, 3.2

mm,

17.1

em,

20;

female luer-fittings, 4 male luer-fittings

Norprene Tubing, 0.8

mm,

17.9

em,

female luer-fittings, 4 male luer-fittings

wall,

10m

wall,

10m

wall,

10m

wall,

10m

4 red lock-rings, 4

4 orange lock-rings, 4

4 yellow lock-rings, 4

20; 4

red

lock-rings, 4

731-8245

731-8246

Fittings

732-8220

731-8221

731-8222

731-8223

731-8224

731-8225

731-8226

731-8228

Norprene Tubing, 1.6 mm, 17.9

em,

20;

4 orange lock-rings, 4

female luer-fittings, 4 male luer-fittings

Norprene Tubing, 3.2 mm, 17.9

em,

20;

4 yellow lock-rings, 4

female luer-fittings, 4 male luer-fittings

Low Pressure Fittings Kit, includes over 250 male and female

luer

connectors, 2- and 3-way stopcocks, and tubing connec-

tors

Female Luer with Barb for 0.8 mm

Female Luer with Barb for 1.6 mm ID Tubing, 25 Female Luer with Barb for 3.2 mm Male Luer with Barb for 0.8 mm Male Luer with Barb for 1.6 mm Male Luer with Barb for 3.2 mm Female-to-Female Luer, 1 0

10

Tubing, 25

10

Tubing, 25

10

Tubing,

10

Tubing, 25

10

Tubing,

25

52

Catalog Number

Product Description

731-8229

731-8230

731-8232

731-8233

732-8102

732-8103

732-8107

732-8300

732-8302

Female-to-Female-to-Female Luer, Male-to-Male Luer, 1 0

Female Luer Plugs, 25

Male Luer Plugs, 25

2-way Polycarbonate Stopcock, 1 0 3-way Polycarbonate Stopcock, 1 0 3-way Nylon Stopcock, 1 0 Straight-through Connector,

25

T-connector, 25

1 0

Econo System Cables

731-8261 Econo System Cable 1, 8-pin mini-DIN to DB-9 connector. For

731-8262

connection of Econo

Pump, Econo UV Monitor,

Econo System Cable 2, 8-pin mini-DIN to 8-pin standard DIN.

For connection of Econo Recorders to the Econo UV Monitor, Econo Controller

Pump, Econo Gradient Monitor, or Econo System

Model 2110 Econo Fraction Collector to the

or

Econo System Controller

731-8263

731-8264

731-8265

731-8266

731-8267

731-8268

Econo System Cable 3, 8-pin mini-DIN to 8-pin mini DIN.

Connects the Econo Pump to the Econo UV Monitor absence of the System

Controller

Econo System Cable 4, 8-pin mini-DIN to banana cable.

connect Econo UV Monitor to most non-Bio-Rad chart recorders.

to the

Cable 4

Model 1327 Econo Recorder

is

also used to connect the Gradient Monitor

in

the

To

Econo System Cable 5, DB-9 connector to bare wires. For

connection of

System components

Econo System Cable

Connects Econo Recorder to non-Econo System detectors

Econo System Cable

Connects Econo

Monitor, Econo Buffer non-Econo System components

Model 2110 Fraction Collector to non-Econo

6,

8-pin standard DIN to bare wires.

7,

8-pin mini-DIN to bare wires.

Pump, Econo

Selector, or Econo System Controller to

UV

Monitor, Econo Gradient

Econo System Cable 8, 8-pin standard DIN to DB-9 connector.

For connection of Econo Recorders to Collector

Model 2110 Fraction

53

Catalog Number

731-8269

Product Description Econo System Cable

1

00. For connection of Pharmacia Frac-1 00 fraction collector

to the Econo

System Controller or Econo Pump

9,

8-pin mini-DIN to Pharmacia Frac-

731-8281

731-8282

731-8283

731-8284

731-8285

Tygon

Norprene

is

Econo System Cable

For connection of Econo Buffer Controller

Econo System Cable 11, 8-pin mini-DIN to lsco DB-9 connec-

tor.

For connection of lsco Retriever

System

Econo System Cable 12, 8-pin

nector. For connection of

System

Econo System Cable 13, 8-pin

connection of

Controller or Econo Pump( does not work with the Foxy 200.)

Econo System Cable 14, 8-pin mini-DIN to Gilson connector,

for connection of or Econo Pump

a registered trademark of Norton Co.

is

a registered trademark of the Norton Co.

Controller or Econo Pump

lsco Foxy collector to the Econo System

10, 8-pin mini-DIN to 11-pin connector.

Gilson FC203 to the Econo System Controller

Selector to the Econo System

II

mini-DIN to lsco DB-15 con-

lsco Retriever

mini-DIN to lsco connector. For

collector to the Econo

II

collector to the Econo

54

55

Bio-Rad

GGG

Laboratories

U.S.

(BOO)

Life Science Group

Ph. Ph.

Ph.

•

Ph. Ph. Ph. Ph. Kingdom

4BIORAD • California (516) 756-2575 • Australia (1)

877 89

01 • Belgium

(905)

712-2771

Germany

03-3534-7515 • 08385-40666 • (65) 4432529 • 46

(0) 735 83 00 • Switzerland

• China

Ph.

089 31884-0 • Italy

Hong

New Spain

Ph.

0800 181134

Zealand

Kong

Ph.

(510) 741-1000 •

Ph.

02-805-5000 • Austria

Ph.

09-385 55

Ph.

(01)

2563148 • France

Ph.

7893300 • The Netherlands

Ph.

09-443 3099 • Singapore

Ph.

(91)

661

Ph.

SIG

New

York

11

• Canada Ph.

(1)

49 60 68

02-21609

70 85 • Sweden

01-810

1•

Japan

16

77 • United

050494 Printed in USA

M7318180 Rev

34

Bio-rad Econo System User Manual

Specifications and Main Features

Frequently Asked Questions

User Manual