Bio-Rad Econo Gradient Pump Kits User Manual

ECONO

PUMP

GRADIENT

Econo Gradient Pump

Instruction Manual

Safety

SAFETY

!

Caution/Warning

Disconnect power to the Econo Gradient Pump before
servicing. No user-serviceable parts are inside the unit.
Refer servicing to Bio-Rad service personnel.

The Bio-Rad Econo Gradient Pump is certified to meet the I.E.C.
1010-1* standard for safety of laboratory equipment. Certified
products are safe to use when operated in accordance with the
instruction manual. This safety certification does not extend to other
chromatography equipment or accessories not I.E.C. 1010 certified,
even when connected to this Bio-Rad Econo Gradient Pump.

This instrument is intended for laboratory use only.

The Bio-Rad Econo Gradient Pump conforms to the “Class A“
standards for Electromagnetic Emissions, intended for laboratory
equipment applications. It is possible that emissions from this product
may interfere with some sensitive appliances when placed nearby or
on the same circuit as those appliances. The user should be aware
of this potential and take appropriate measures to avoid interference.

This instrument should not be modified or altered in any way.
Alteration of this instrument will void the manufacturer’s warranty,
void the I.E.C. 1010 certification, and create a potential safety hazard
for the user.

Bio-Rad is not responsible for any injury or damage caused by the
use of this instrument for purposes other than for which it is intended
or by modifications of the instrument not performed by Bio-Rad or an
authorized agent.

*I.E.C. 1010 is an internationally accepted electrical safety standard for
laboratory instruments.

i

Table of Contents

TABLE OF CONTENTS

Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i

Section 1 Introduction . . . . . . . . . . . . . . . . . . . . 1

Section 2 Unpacking and Setting Up . . . . . . . . . 2

2.1 Unpacking Instructions . . . . . . . . . . . . . 2

2.2 Voltage Conversion . . . . . . . . . . . . . . . 3

Section 3 Physical Description and Control Features 4

3.1 Front Panel Functions . . . . . . . . . . . . . 4

3.2 Rear Panel Functions . . . . . . . . . . . . . . 8

Section 4 Tubing Selection and Installation . . . . 10

4.1 Tubing Selection . . . . . . . . . . . . . . . . . . 10

4.2 Tubing Installation . . . . . . . . . . . . . . . . 12

4.3 Platen Adjustment . . . . . . . . . . . . . . . . 13

Section 5 System Operation . . . . . . . . . . . . . . . . 14

5.1 Remote Mode . . . . . . . . . . . . . . . . . . . . 14

5.2 Setup Mode . . . . . . . . . . . . . . . . . . . . . 14

5.3 Manual Mode . . . . . . . . . . . . . . . . . . . . 20

5.3.1 Setting Flow Rate or Motor Speed 20

5.3.2 Setting Buffer Gradient . . . . . . . 20

5.3.3 Setting Auxiliary Valve Control . 21

5.4 Program Mode . . . . . . . . . . . . . . . . . . . 21

5.5 Running a Program . . . . . . . . . . . . . . . 23

5.5.1 Holding a Run . . . . . . . . . . . . . 24

5.5.2 Pausing a Run . . . . . . . . . . . . . 24

5.5.3 Aborting a Run . . . . . . . . . . . . . 24

5.5.4 Power Failure While in Run Mode 25

Section 6 Operation with other Bio-Rad Instruments 24

Section 7 Cleaning and Maintenance . . . . . . . . . 27

7.1 Fuse Replacement and Voltage Conversion 33

Section 8 Troubleshooting . . . . . . . . . . . . . . . . . 35

Section 9 Embedded Functions . . . . . . . . . . . . . 38

9.1 Firmware Confirmation . . . . . . . . . . . . . 38

9.2 Reset . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Appendix A Technical Specifications . . . . . . . . . . 39

Appendix B Connection to Other Instruments . . . 41

iii

Appendix C Warranty . . . . . . . . . . . . . . . . . . . . . . . 43

Appendix D Ordering Information . . . . . . . . . . . . . 44

Table of Contents

iv

Introduction

1.0 INTRODUCTION

The Econo Gradient Pump (EGP) is a two-channel, bi-directional,
variable speed peristaltic pump for low-pressure chromatography and
general laboratory use. It offers a full range of features to facilitate
ease of use as a stand-alone pump or as a part of the BioLogic
Duo-Flow System running software version 3.0 or later..

As a stand-alone pump, the EGP delivers flow rates from 2 µl/min to
40 ml/min using a single channel of 0.51 mm or dual channels of 3.2
mm ID tubing respectively. The EGP has the ability to self-calibrate
the flow rate for 0.51, 0.8, 1.6, and 3.2 mm ID tubing, displaying pump
output in ml/min. The pump can be programmed to start or stop
Bio-Rad fraction collectors and chart recorders.

A membrane key panel with graphic icon displays allows easy user
interface. The EGP will store one program in its non-volatile memory.

ECONO GRADIENT PUMP

Reverse

Remote
Setup
Manual
Program

Mode

RunPurge

Next

Cancel

Step

Figure 1-1. Econo Gradient Pump.

1

Unpacking

2.0 UNPACKING AND SETTING UP

2.1 UNPACKING INSTRUCTIONS

Carefully remove the unit from the shipping box, lifting from the
handle on the back of the pump or from the bottom of the instrument.
Remove the plastic bag and inspect the instrument for any external
damage. Figure 2-1 shows the contents of the box.

Model EGP
Econo Pump

Low-Pressure
Fittings Kit

Power Cord

Pumphead
Tubing

Figure 2-1. Parts supplied with the Econo Gradient Pump.

2

System
Tubing

Unpacking

Your Econo Gradient Pump was carefully tested at the factory and
was shipped in good working order. If any part is missing or
damaged, contact your local Bio-Rad office immediately.

2.2 VOLTAGE CONVERSION

Warning: The Econo Gradient Pump is shipped in its 100/120 V

configuration. To operate at 220/240 V, refer to the procedure in
section 7, Cleaning and Maintenance, to make the conversion.
Failure to follow this procedure may result in damage to the unit and
invalidation of the warranty.

3

3.0 PHYSICAL DESCRIPTION AND CONTROL
FEATURES

3.1 FRONT PANEL FUNCTIONS

Figure 3-1. Front Panel Controls and Displays.

Description

4

Platen

Arrow Keys

ECONO GRADIENT PUMP

Reverse

Remote
Setup

Mode

Manual
Program

LCD Display

RunPurge

Next

Cancel

Soft Keys

Step

Front Panel Function

[LCD; see figure 3-1] LCD Display

This display has two rows of 16 characters.
Different windows will appear on this display
depending on the requests made by the
user. Some windows will display operating
parameters, while others will allow entry of
variables such as flow rates, valve position
and programming steps. Choices are shown
in the lower row, above one of the three soft
keys. A selection is indicated in uppercase
letters.

Soft Keys

These keys are used to select choices
displayed on the LCD. A selection is
indicated in uppercase letters. Pressing and
holding a soft key and an arrow key acts to
accelerate the action of the arrow keys.

Arrow Keys

These keys are used for setting system
parameters when programming the system,
and for selecting which parameter to display
when running the pump. A value input with
the arrow key can be erased or accepted
with the Cancel and Next keys respectively.
Pressing and holding an arrow key will
accelerate the action of the arrow keys.

Next Key

Pressing Next will accept any values
displayed in the current window and
advance to the next window.

Description

5

Next

Description

Front Panel Function

Cancel Key

Cancel

Pressing Cancel will clear an entry that has
been input with either the arrow or soft keys.
If no entry has been made, the previous
window will be displayed. This allows
passage through the windows in the reverse
direction.

Run/Stop Key

Run

This key is used to start or stop the pump in
both Manual mode and Program mode
operation. The pump will run in the clockwise
direction by factory default. The green and
red LEDs are illuminated when the Econo
Gradient Pump is running or stopped,
respectively. The direction can be changed
using the Reverse Direction key, when the
pump is stopped.

Reverse

6

Reverse Direction Key

This key changes the direction of pump
head rotation. The Econo Gradient Pump
must be stopped before the direction can be
changed. This key is not operable when the
pump is running. The yellow and orange
LEDs are illuminated when the pump is
running clockwise (CC) and counter­clockwise (CCW), respectively.

Front Panel Function

Purge Key

Purge

Mode

This key is used to prime and purge tubing
lines without disturbing the primary speed
setting of the pump. When Purge is pressed,
the pump will run at maximum speed (25
rpm), leaving the connected Gradient Mixer
and Aux valve operating and the fraction
collector and chart recorder off. The yellow
purge LED is illuminated when purging. This
key is not operable when the pump is
running in Program mode. Purge can be
stopped by pressing the Purge or Run keys.

Mode Key

Four different operation modes are
available: Remote, Setup, Manual, and
Program modes. These modes are
described in Section 6, System Operation.

Description

Step Key and LEDs

Step

[Platen; see figure 3-1] Platen Screw

Up to six program steps can be used. This
key selects the active step for programming.
The Step LEDs indicate which of the six
steps is active.

This is the screw on the pumphead
assembly. Proper adjustment of the platen
pressure increases flow stability, minimizes
flow pulsation, and prolongs the life of the
tubing. It should be adjusted 3-5 turns,
depending on tubing diameter. Refer to
section 4.3 for more details.

7

Description

3.2 REAR PANEL FUNCTIONS

Made in U.S.A.

Model No. Econo Gradient Pump
Serial No. 491 BR
AC Voltage
Max Current

0.4 A

0.2 A

Frequency

50-60 Hz

50-60 Hz

Fuse Type

0.4 A

0.2 A

EN

61010-1

Power

Entry Module

Fuse Holder

CAUTION

WARNING

Warning: Disconnect supply before servicing.
Advertissement:: Couper l'alimentation avant
l'entretien et depannage.

INSTR BUS

INSTR BUS

AUX VALVE

I/O

MIXER

Instrument
Bus

Power Switch

(Located

Under the Unit)

Figure 3-2. Rear panel connectors.

Display Function

Power Entry Module

Grounded 3-pin receptacle for the power
cord. Also contained within is a two-position
(100-120 V/220-240 V) fuse holder.

Gradient Mixer Connector

This 8-pin outlet controls the Gradient Mixer
used for making binary linear gradients.

Mixer
Aux Valve

I/O

8

Display Function

Aux Valve Connector

This 8-pin connector controls auxiliary
valves such as the optional SV-3 Diverter
valve or the optional Splitter valve.

I/O Connector

This 8-pin outlet provides start signals to the
Model 1327 Chart Recorder, the Model 2110
Fraction Collector, or the Model 2128
Fraction Collector. Simultaneous control of
both the Model 2110 and the Model 1327, or
the Model 2128 and the Model 1327, can be
accomplished via the 2110-Y cable and the
2128-Y cable respectively. I/O connector
pinouts are given in Appendix B. A TTL input
is also present which allows the pump to be
started remotely.

Description

[base; see figure 3-2] The underside of the Econo Gradient Pump

contains the power switch.

9

4.0 TUBING SELECTION AND INSTALLATION

4.1 TUBING SELECTION

The Econo Gradient Pump may be used with most flexible tubing
having an inner diameter less than or equal to 3.2 mm (1/8”), and a
wall thickness of 1.0 mm or less, including Silicone, Tygon, and
PharMed. Silicone tubing, the most inert of the three, is suitable for
aqueous and polar solutions. Tygon is suitable for most aqueous
solutions. It will generally have the shortest lifetime of the three and
should not be used for the pumphead. PharMed is the longest lasting
of the three, and will provide the most consistent flow rate over time.
Figure 4-1 shows approximate flow rate ranges with different tubing
sizes. Flow rates above 20 ml/min may be obtained by plumbing two
channels of 3.2 mm tubing through the pump and joining them at the
output.

Very low flow rates can be achieved with tubing having ID as small as

0.51 mm. Tubing this small must be jacketed inside larger tubing and
requires an adapter. These items are included in the optional splitter
valve kit.

Figure 4-1. Approximate Flow Rate Ranges.

Tubing length and the amount of tubing prestretch have a significant
effect on both flow rate calibration and reproducibility of flow. The
PharMed tubing supplied with the Econo Gradient Pump has been
cut to a predetermined length to accommodate tubing prestretch.
(Silicon tubing in precut lengths is also available from Bio-Rad. See
ordering information in Appendix D.)

Tubing Selection

10

m

TUBING SIZE

(ID x WALL)

.001 .002 .005 .01

0.51 x 0.8 mm

0.8 x 0.8 mm

1.6 x 0.6 mm

3.2 x 0.8 mm

FLOW RATE RANGE (ml/min)

.02 .05 .10 20.20 .5 1.0 2510

ml/

Tubing Selection

When using tubing other than precut tubing from Bio-Rad, the tubing
must be cut to the following lengths to accommodate prestretch:

Tubing Length

Tygon, PharMed 179 mm +/- 1.5 (7.04” +/- 0.05)
Silicone 171 mm +/- 1.5 (6.75” +/- 0.05)

Install tubing onto the pump head as described in Section 4.2.

Warning: If using tubing other than the type supplied by Bio­Rad, make sure the wall thickness is not greater than 1.0
mm. Using tubing with a greater wall thickness can damage
the pump and void your warranty.

Figure 4-2. Tubing installation.

11

Tubing Selection

4.2 TUBING INSTALLATION

1. Referring to Figure 4-2, pull the platen cam lever away from the
pump head to unlock the platen and slide the platen away from
the pump head frame assembly, exposing the rollers.

2. Slip a lock-ring onto one end of the tubing. See below for the size
and color of each lock-ring. Insert a barbed female luer fitting into
the same end until the tubing reaches the flange of the fitting.
Clamp the luer-fitting into place by sliding the lock ring along the
tubing over the barbed fitting (see Figure 4-3). Repeat on the
other end of tubing.

Note: Use of lock-rings is required only when operating at
pressures above 10 psi.

Tubing ID Lock-Ring Color

0.51 mm (.02”)* None Required

0.8 mm (1/32”) Red

1.6 mm (1/16”) Orange

3.2 mm (1/8”) Yellow

*

The 0.51 mm ID tubing is placed inside the 3.2 mm ID tubing, thus no lock­rings are required for the smaller tubing.

12

Figure 4-3. Attachment of tubing fittings.