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Criterion XT™Precast Gel
Instruction Guide
CCaattaalloogg NNuummbbeerr
334455--99889988
For Technical Service Call Your Local Bio-Rad Office
or in the US, Call 1-800-4BIORAD (1-800-424-6723)
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Table of Contents
Section 1 General Information.............................................................................................. 1
1.1 Introduction........................................................................................................................................... 1
1.2 Criterion XT Precast Gels...................................................................................................................... 2
1.3 Criterion System Specifications............................................................................................................. 4
1.4 Criterion XT Comb Configurations......................................................................................................... 4
Section 2 Setup and Basic Operation................................................................................... 5
2.1 Setting Up and Running Criterion XT Gels.............................................................................................5
2.2 Opening Criterion XT Cassettes and Removing the Gels...................................................................... 6
Section 3 SDS-PAGE and Native PAGE................................................................................. 7
3.1 Criterion XT Gel Selection Guide........................................................................................................... 7
3.2 Bis-Tris Gel Composition...................................................................................................................... 9
3.3 Tris-Acetate Gel Composition............................................................................................................... 9
3.4 Criterion XT Buffers and Reagents........................................................................................................ 9
3.5 Sample Preparation..............................................................................................................................10
3.6 Running Conditions .............................................................................................................................10
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Section 4 2-D Electrophoresis............................................................................................ 11
4.1 Equilibration....................................................................................................................................... 11
4.2 Agarose Overlay ............................................................................................................................... 11
Section 5 Staining and Detection....................................................................................12–13
5.1 SDS-PAGE and Native PAGE Detection.........................................................................................12–13
Section 6 Blotting................................................................................................................ 14
Section 7 Troubleshooting..............................................................................................15–16
Section 8 Ordering Information......................................................................................17–22
8.1 Criterion XT Gels................................................................................................................................ 17
8.2 Criterion XT Buffers and Kits ............................................................................................................. 17
8.3 Other Related Products..................................................................................................................... 17
8.4 Criterion Gels.................................................................................................................................18–19
8.5 Criterion Gel Accessories................................................................................................................... 20
8.6 Protein Standards ............................................................................................................................. 20
8.7 Detection Reagents........................................................................................................................... 21
8.8 Blotting Membranes.......................................................................................................................... 22
8.9 Equipment......................................................................................................................................... 22
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Section 1
General Information
1.1 Introduction
Criterion is the next generation of dedicated precast gel systems. The innovative, easy-to-use design
produces superior resolution while allowing you to run more samples per gel. Compared to any other precast
gel system, Criterion produces more results while providing significant cost and time savings. Some of the
unique features and benefits provided are:
• 12 month shelf life for Bis-Tris gels
• 8 month shelf life for Tris-acetate gels
• Room temperature storage for Bis-Tris gels
• Easy sample preparation without extra anti-oxidant addition steps
• Patented integral buffer chamber that eliminates buffer leaks
• Up to 26 sample capacity per gel
• Flexibility to run one or two gels
• Multichannel pipet compatible gels
• Outlined and numbered wells that simplify sample loading
• J-foot that improves gel drying and blotting results
US Patents #5,073,246, #5,656,145, #6,093,301 and other patents issued and pending.
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1.2 Criterion XT Precast Gels
Criterion XT precast gels are formulated at pH near neutrality to optimize gel matrix stability, significantly
delaying acrylamide hydrolysis, which occurs in traditional Laemmli systems. Specially optimized buffers
result in tight, consistently resolved bands throughout the life of the gel.
This versatile system allows the separation of small to large proteins using just two gel buffer systems:
Criterion XT Bis-Tris precast gels for small to mid-sized proteins and Criterion XT Tris-acetate precast gels for
large proteins.
The Criterion XT Bis-Tris gels are based on a Bis-Tris.HCl buffer system (pH 6.4) that uses discontinuous
chloride and MES or MOPS ion fronts to form moving boundaries to stack and then separate denatured
proteins by size. The chemistry of the XT Bis-Tris gels allows maximum stability and consistent results for a
minimum of one year. Running the same XT Bis-Tris gels with the XT MES denaturing running buffer or the XT
MOPS denaturing running buffer will produce different migration patterns. A combination of these two running
buffers and our three XT Bis-Tris gels can produce up to six different migration patterns in the small and midsize range.
The Criterion XT Tris-acetate gels are based on a Tris-acetate buffer system (pH 7.0). It uses discontinuous
acetate and Tricine ion fronts to form moving boundaries to stack and then separate large denatured proteins
by molecular weight. The Criterion XT Tris-acetate gels can also be used to separate proteins by their chargeto-mass ratio (under native-PAGE conditions). This is possible because the XT Tris-acetate gels are made
without SDS, allowing the sample buffer and running buffer to dictate the separation mechanism. The
nonreducing and nondenaturing environment of native PAGE allows the detection of biological activity and
can improve antibody detection. Native PAGE can also be used to resolve multi-protein bands where
molecular mass separation by SDS-PAGE would reveal only one and for the separation of intact protein
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complexes. Separation by native PAGE with XT Tris-acetate gels uses discontinuous acetate and glycine
ion fronts to form moving boundaries to stack and separate proteins by both size and charge.
Protein samples for the Criterion XT precast gel system are prepared in a reducing denaturing sample buffer.
The sample buffer contains XT reducing agent, a pH neutralized and stabilized solution of TCEP as the
reducing agent; heat and SDS are used to denature the proteins. In addition, the use of TCEP in combination
with Bio-Rad’s optimized running buffers maintains proteins in a fully reduced state during the electrophoresis
run, eliminating the need for an anti-oxidant in the upper buffer chamber. Criterion XT Tris-acetate precast
gels can also be used for native PAGE. Proteins are prepared in a nonreducing, nondenaturing sample buffer,
which maintains the proteins’ native structure and charge density.
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1.3 Criterion System Specifications
Gel material Polyacrylamide
Gel dimensions (W x L) 13.3 x 8.7 cm
Gel thickness 1.0 mm
Resolving gel height 6.5 cm
Cassette dimensions (W x L) 15.0 x 10.6 cm
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Upper running buffer volume 60 ml
Lower running buffer volume 800 ml
Storage conditions Bis-Tris gels: Store flat at ambient temperature; DO NOT FREEZE
Tris-acetate gels: Store flat at 4°C; DO NOT FREEZE
Gel shelf life 12 months for Bis-Tris gels; 8 months for Tris-acetate gels
1.4 Criterion XT Comb Configurations
Comb Load Volume Comments
12+2 well 45 µl with two 15 µl reference wells Multichannel pipet compatible
18-well 30 µl
26-well 15 µl Multichannel pipet compatible
Prep+2 well 800 µl with two 15 µl reference wells
IPG 11 cm ReadyStrip
IPG+1 well 11 cm ReadyStrip IPG strip with one 15 µl reference well
IPG strip
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Section 2
Setup and Basic Operation
2.1 Setting Up and Running Criterion XT Gels
1. Each Criterion XT gel is packaged individually in a plastic storage tray. Remove the cover by gently pulling
the corner tab up and diagonally across the package. Remove the gel from the package.
2. Remove the comb and gently rinse the wells with ddH
2
O or running buffer.
3. Remove the tape from the bottom of the cassette by pulling the
tab across the gel.
4. Insert the Criterion XT gel into one of the slots in the Criterion cell
tank. Ensure that each integral buffer chamber faces the center of
the cell.
5. Fill each integral buffer chamber with 60 ml running buffer.
6. Load samples using a Hamilton syringe or a pipet with gel loading
tips. A sample loading guide can be placed on the outer edge of
the cassette to aid in aligning pipet tips with the wells. This is
especially useful with multichannel pipets.
7. Fill each half of the lower buffer tank with 400 ml of running buffer
to the marked fill line.
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8. Place the lid on the tank, aligning the color-coded banana plugs and jacks. See section 3.6 for power
conditions.
2.2 Opening Criterion XT Cassettes and
Removing the Gels
1. After electrophoresis is complete, turn off the power supply
and disconnect the electrical leads.
2. Remove the lid from the tank and remove the Criterion XT
gel(s) from the cell. Pour off and discard the upper running
buffer.
3. Invert the cassette and place the integral buffer chamber over
the cassette-opening tool built into the Criterion cell lid.
4. Firmly press down on the cassette to crack the cassette welds
on both sides of the cassette. The cassette will split open
approximately 1/3 of the way.
5. Alternatively, the gel cassette can be opened by sliding the
tapered back of the comb into the slits on either side of the
cassette.
6. Pull the two halves of the cassette apart to completely expose
the gel.
7. Remove the gel by either floating the gel into a fixing or staining
solution or by carefully lifting the gel from the cassette.
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Section 3
SDS-PAGE and Native PAGE
3.1 Criterion XT Gel Selection Guide
Criterion XT gels are available in a wide selection of single acrylamide percentages and gradients for the
separation of proteins by SDS-PAGE or native PAGE.
Optimal Separation
Bis-Tris Gels With XT MES Running Buffer With XT MOPS Running Buffer
10% 2.5–200 kD 14–220 kD
12% 1–30 kD 6–66 kD
4–12% 2.5–200 kD 10–300 kD
Tris-Acetate Gels* With XT Tricine Running Buffer With Tris/Glycine Running Buffer
7% 36–200 kD N/A
3–8% 40–400 kD N/A
*Because Criterion XT Tris-acetate gels are made without SDS, they can be used to separate proteins by both SDS-PAGE and native PAGE.
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Criterion XT Protein Migration Chart
250
150
100
250
150
100
75
50
37
25
20
15
10
10% 12% 4–12% 10% 12% 4–12% 7% 3–8%
Bis-Tris gels with
XT MES running buffer:
ideal for
SMALL proteins
250
150
75
100
50
37
25
20
15
10
75
50
37
25
20
15
10
250
150
100
250
150
100
75
50
37
25
20
Bis-Tris gels with
XT MOPS running buffer:
ideal for
MID-SIZE proteins
250
150
75
100
50
37
25
20
15
10
75
50
37
25
20
15
250
150
100
Tris-acetate gels with
XT Tricine running buffer:
250
150
75
100
50
37
LARGE proteins
75
50
37
ideal for
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3.2 Bis-Tris Gel Composition
Gel buffer Bis-Tris.HCl, pH 6.4
Cross linker 5% C
Stacking gel 4% T, 5% C
Storage buffer Bis-Tris.HCl, pH 6.4
Shelf life 12 months; individual expiration date is printed on each cassette; store flat at ambient temperature
3.3 Tris-Acetate Gel Composition
Gel buffer Tris-acetate, pH 7.0
Cross linker 3.8% C
Stacking gel 4% T, 3.8% C
Storage buffer Tris-acetate, pH 7.0
Shelf life 8 months; individual expiration date is printed on each cassette, store flat at 4°C
3.4 Criterion XT Buffers and Reagents
Bis-Tris running buffer 20x XT MOPS (dilute to 1x) or XT MES (dilute to 1x)
for SDS-PAGE For separation of mid-size proteins For separation of small proteins
Catalog #161-0788 Catalog #161-0789
Tris-acetate running buffer 20x XT Tricine (dilute to 1x)
for SDS-PAGE For separation of large proteins
Catalog #161-0790
Tris-acetate running buffer 10x Tris-Glycine (dilute to 1x)
for Native-PAGE Catalog #161-0732
XT sample buffer Catalog #161-0791
XT reducing agent Catalog #161-0792
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3.5 Sample Preparation
Determine the appropriate protein concentration of your sample based on the detection method and load
volume used. (See section 4.1 for approximate stain sensitivities.) XT sample buffer is a 4x concentrate and
can be used with both dilute and concentrated samples. Refer to the sample preparation guide below:
Sample Preparation Guide
SDS-PAGE Native-PAGE
25 µl XT sample buffer 50 µl Native sample buffer
5 µl XT reducing agent x µl sample
x µl sample
Make up to 100 µl with ddH20 Make up to 100 µl with ddH2O
Heat sample at 95°C for 5 min. Do not heat sample
3.6 Running Conditions
Gel type (for SDS-PAGE) (for SDS-PAGE) (for SDS-PAGE) (for Native-PAGE)
Running buffer XT MOPS XT MES XT Tricine Tris/Glycine
Power conditions 200 V constant 200 V constant 150 V constant 200 V constant
Run time 60 min 45 min 65 min 75 min
Starting current 165–175 mA/gel 185–200 mA/gel 170–180 mA/gel 70–80 mA/gel
Final current 60–70 mA/gel 90–110 mA/gel 85–95 mA/gel 25–35 mA/gel
Bis-Tris Bis-Tris Tris-Acetate Tris-Acetate
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Section 4
2-D Electrophoresis
4.1 Equilibration
Use existing equilibration protocols as described in the ReadyPrep 2-D Starter Kit (catalog #163-2105 or
bulletin 411009) or existing protocols and buffers used for Tris-HCl gels.
4.2 Agarose Overlay
Make a solution of 0.6% low melt agarose and 0.002% Bromophenol blue. To make 10 ml of the agarose
overlay, mix 9.5 ml of the above agarose with 0.5 ml of 20x XT Running Buffer. Use the XT Running Buffer that
will be used to run the second dimension gel.
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Section 5
Staining and Detection
5.1 SDS-PAGE and Native PAGE Detection
Total Protein Gel Stain
Method Sensitivity Optimal Protein Load Advantages Disadvantages
Coomassie Blue R-250 36–47 ng ~0.5 µg/band Laboratory standard Requires MeOH
™
Bio-Safe
stain no MeOH Coomassie R-250
Zinc stain 6–12 ng ~0.2 µg/band High-contrast, fast, Negative stain, must
Silver Stain Plus
Silver stain 0.6–1.2 ng ~0.01 µg/band Stains complex Not mass
SYPRO Orange protein 4–8 ng ~0.2 µg/band Will not stain nucleic Optimization required
stain acids; mass for maximum
SYPRO Ruby protein 1–10 ng ~0.2 µg/band Broad dynamic range, Requires imaging
gel stain simple robust protocol instrument for
Coomassie 8–28 ng ~0.5 µg/band Nonhazardous, uses More steps than
reversible stain be photographed;
™
kit 0.6–1.2 ng ~0.01 µg/band Simple, robust, mass Will not stain
spectrometry compatible glycoproteins
proteins: i.e., glycoproteins spectrometry
and lipoproteins compatible
spectrometry compatible sensitivity
SDS-PAGE only
maximum sensitivity
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Total Protein Blot Stain
Method Sensitivity Optimal Protein Load Advantages Disadvantages
SYPRO Ruby protein 2–8 ng ~0.2 µg/band Compatible with mass Multiple-step protocol;
blot stain spectrometry, Edman-based Requires imaging
sequencing, and standard instrument for
immunological procedures maximum sensitivity
Colloidal gold stain 1 ng ~0.1 µg/band Sensitive, one step Not compatible with
nylon membranes
Enhanced colloidal 10–100 pg ~0.1 µg/band Increases sensitivity of Multiple steps
gold detection kit colloidal gold stain
Amido Black 100–1,000 ng ~5 µg/band Standard membrane Low sensitivity
stain, economical
Immunoblot Detection
Method Sensitivity Optimal Protein Load Advantages Disadvantages
4CN colorimetric (HRP)* 500 pg ~0.25 µg/band Fast detection Results may fade
DAB colorimetric (HRP) 500 pg ~0.25 µg/band Fast detection Contains toxic
chemicals
Opti-4CN colorimetric 100 pg ~0.05 µg/band Color does not fade More expensive than
(HRP) 4CN
Amplified Opti-4CN
™
10 pg ~0.005 µg/band High sensitivity, low Amplification requires
colorimetric (HRP) background additional steps
BCIP/NBT colorimetric 100 pg ~0.05 µg/band Sensitive; multiple antigens May detect endogenous
(AP) enzyme activity
Amplified AP* 10 pg ~0.005 µg/band High sensitivity Amplification requires
additional steps
Immun-Star
™
10 pg ~0.005 µg/band Long-lasting signal, Requires visualization
chemiluminescent (AP) short and multiple on film or instrumentation
exposures possible
*(HRP) horseradish peroxidase; (AP) alkaline phosphatase
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Section 6
Blotting
Criterion XT gels are blotted using the same buffers and protocols used to blot Tris-HCl and other
polyacrylamide gels. Please refer to the Criterion blotter instruction manual (bulletin 4006190) for detailed
instructions on how to blot gels. Tris/Glycine (Towbin) transfer buffer is recommended for western transfer of
the Criterion XT pre-cast gels.
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Section 7
Troubleshooting
Improper storage of Criterion XT gels can produce numerous artifacts. Criterion XT Bis-Tris gels should be
stored flat at ambient temperature. Criterion XT Tris-acetate gels should be stored flat at 4°C. Avoid freezing. If
you suspect your gels have been stored improperly, DO NOT USE THEM.
Problem Possible Cause Solution
Samples do not migrate into gel Tape at the bottom of the cassette Remove tape
Bands “smile” across gel, band pattern Excess heating of gel Check buffer composition
curves upward at both sides of the gel
Skewed or distorted bands, lateral Excess salt in samples Remove salts from sample by dialysis or
band spreading desalting column prior to sample
not removed
Insufficient buffer in integral buffer chamber Fill integral buffer chamber with 60 ml
running buffer
Insufficient lower electrode buffer Fill both halves of the lower buffer tank with
400 ml running buffer when running two gels
Electrical disconnection Check electrodes and connections
Completely fill both halves of the lower buffer
tank with 400 ml running buffer when running
two gels
Do not exceed recommended running
conditions
preparation
Insufficient sample buffer Check buffer composition and dilution
or wrong formulation instructions
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Problem Possible Cause Solution
Vertical streaking Samples overloaded Dilute sample
Selectively remove predominant protein in
the sample
Sample precipitation Centrifuge samples to remove
Gels run too fast, provide poor resolution, Running buffer is too concentrated Check buffer composition
and gel temperature is too high
Artifact bands at ~60–70 kD Possible skin keratin contamination Wear gloves while cleaning all dishware
particulates prior to sample loading
and while handling and loading gel
Filter all solutions through nitrocellulose
Use 10% iodoacetamide to eliminate
keratin bands
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Section 8 Ordering Information
8.1 Criterion XT Gels
Criterion XT Bis-Tris Gels 12+2 Well 18-Well 26-Well Prep Well IPG+1 Well IPG Well
10% Bis-Tris 345-0111 345-0112 345-0113 345-0114 345-0115 345-0116
12% Bis-Tris 345-0117 345-0118 345-0119 345-0120 345-0121 345-0122
4–12% Bis-Tris 345-0123 345-0124 345-0125 345-0126 345-0127 345-0128
Criterion XT Tris-Acetate Gels 12+2 Well 18-Well 26-Well Prep Well IPG+ 1 Well IPG Well
3–8% Tris-Acetate 345-0129 345-0130 345-0131 345-0132 345-0133 345-0134
7% Tris-Acetate 345-0135 345-0136 345-0137 345-0138 345-0139 345-0140
8.2 Criterion XT Buffers and Kits
Catalog # Description
161-0788 XT MOPS Running Buffer, 20x, 500 ml
161-0789 XT MES Running Buffer, 20x, 500 ml
161-0790 XT Tricine Running Buffer, 20x, 500 ml
161-0791 XT Sample buffer, 4x, 10 ml
161-0792 XT Reducing Agent, 20x, 1 ml
161-0793 XT MOPS Buffer Kit, includes 500 ml 20x XT MOPS running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent
161-0796 XT MES Buffer Kit, includes 500 ml 20x XT MOPS running buffer, 10 ml 4x XT sample buffer, 1 ml 20 x XT reducing agent
161-0797 XT Tricine Buffer Kit, includes 500 ml 20x XT MOPS running buffer, 10 ml 4x XT sample buffer, 1 ml 20x XT reducing agent
8.3 Other Related Products
161-0738 Native Sample Buffer, 30 ml
161-0734 10x Tris/Glycine, 1 L
161-0404 Bromophenol Blue, 10 g
161-0311 Certified Low-Melt Agarose, 25 g
163-2107 ReadyPrep 2-D Starter Kit Equilibration Buffer I, with DTT
163-2108 ReadyPrep 2-D Starter Kit Equilibration Buffer II, with DTT
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8.4 Criterion Gels
Criterion Tris-HCl Gels 45 µl Samples 30 µl Samples 15 µl Samples 800 µl Samples 11 cm IPG Strip 11 cm IPG Strip
5% Tris-HCl 345-0001 345-0002 345-0003 345-0004 - -
7.5% Tris-HCl 345-0005 345-0006 345-0007 345-0008 - -
10% Tris-HCl 345-0009 345-0010 345-0011 345-0012 345-0013 345-0101
12.5% Tris-HCl 345-0014 345-0015 345-0016 345-0017 345-0018 345-0102
15% Tris-HCl 345-0019 345-0020 345-0021 345-0022 - 18% Tris-HCl 345-0023 345-0024 345-0025 345-0026 - 4–15% Tris-HCl 345-0027 345-0028 345-0029 345-0030 345-0031 345-0103
4–20% Tris-HCl 345-0032 345-0033 345-0034 345-0035 345-0036 345-0104
8–16% Tris-HCl 345-0037 345-0038 345-0039 345-0040 345-0041 345-0105
10.5–14% Tris-HCl 345-9949 345-9950 345-9951 345-9952 345-9953 345-0106
10-20% Tris-HCl 345-0042 345-0043 345-0044 345-0045 345-0046 345-0107
12+2 Well 18-Well 26-Well Prep+2 Well IPG Well IPG+1 Well
Criterion TBE Gels 45 µl Samples 30 µl Samples 15 µl Samples 800 µl Samples
5% TBE 345-0047 345-0048 345-0049 345-0050
10% TBE 345-0051 345-0052 345-0053 345-0054
15% TBE 345-0055 345-0056 345-0057 345-0058
4–20% TBE 345-0059 345-0060 345-0061 345-0062
Criterion Peptide Gels 45 µl Samples 30 µl Samples 15 µl Samples 800 µl Samples
16.5% Peptide 345-0063 345-0064 345-0065 345-0066
10–20% Peptide 345-0067 345-0068 345-0069 345-0070
12+2 Well 18-Well 26-Well Prep+2 Well
12+2 Well 18-Well 26-Well Prep+2 Well
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12+2 Well 18-Well 26-Well Prep+2 Well
Criterion IEF Gels 45 µl Samples 30 µl Samples 15 µl Samples 800 µl Samples
IEF pH 3–10 345-0071 345-0072 345-0073 345-0074
IEF pH 5–8 345-0075 345-0076 345-0077 345-0078
12+2 Well 18-Well 26-Well Prep+2 Well
Criterion Zymogram Gels 45 µl Samples 30 µl Samples 15 µl Samples 800 µl Samples
10% Zymogram, gelatin 345-0079 345-0080 345-0081 -
12.5% Zymogram, casein 345-0082 345-0083 345-0084 -
12+2 Well 18-Well 26-Well Prep+2 Well
Criterion TBE-Urea Gels 45 µl Samples 30 µl Samples 15 µl Samples 800 µl Samples
5% TBE-Urea 345-0085 345-0086 345-0087 10% TBE-Urea 345-0088 345-0089 345-0090 15% TBE-Urea 345-0091 345-0092 345-0093 -
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8.5 Criterion Gel Accessories
345-9920 Criterion Staining/Blotting Trays, 12
345-9901 Criterion Empty Cassettes, 1.0 mm with 12+2 comb, 10
345-9902 Criterion Empty Cassettes, 1.0 mm with 18-well comb, 10
345-9903 Criterion Empty Cassettes, 1.0 mm with 26-well comb, 10
345-9904 Criterion Empty Cassettes, 1.0 mm with prep+2 comb, 10
345-9905 Criterion Empty Cassettes, 1.0 mm with IPG comb, 10
165-6006 Criterion Sample Loading Guide, 12+2 well, 1
165-6007 Criterion Sample Loading Guide, 18-well, 1
165-6008 Criterion Sample Loading Guide, 26-well, 1
8.6 Protein Standards
161-0362 Precision Plus Protein™Unstained Standards (10–250 kD), 500 µl, 100 applications
161-0373 Precision Plus Protein All Blue Standards (10–250 kD), 500 µl, 100 applications
161-0317 SDS-PAGE Standards, broad range, 200 µl
161-0303 SDS-PAGE Standards, high range, 200 µl
161-0304 SDS-PAGE Standards, low range, 200 µl
161-0324 Kaleidoscope Prestained Standards, broad range, 500 µl
161-0325 Kaleidoscope Polypeptide Standards, 500 µl
161-0326 Polypeptide SDS-PAGE Standards (1.4–26.6 kD), 200 µl, 400 applications
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8.7 Detection Reagents
Total Protein Gel Stains Total Protein Blot Stains
161-0436 Coomassie Blue R-250 Stain Solution, 1 L 170-3127 SYPRO Ruby Protein Blot Stain, 200 ml
161-0438 Coomassie Blue R-250 Destain Solution, 1 L 170-6527 Colloidal Gold Total Protein Stain, 500 ml
161-0400 Coomassie Brilliant Blue R-250, 10 g 170-6517 Enhanced Colloidal Gold Detection Kit
161-0786 Bio-Safe Coomassie Stain, 1 L 161-0402 Amido Black 10B, 25 g
161-0440 Zinc Stain and Destain Kit
161-0449 Silver Stain Plus Kit
161-0443 Bio-Rad Silver Stain Kit
170-3120 SYPRO Orange Protein Stain, 500 µl
170-3125 SYPRO Ruby Protein Gel Stain, 1 L
161-0434 IEF Gel Staining Solution, 1 L
Immunoblot Detection
170-6431 HRP Conjugate Substrate Kit, 4CN
170-6535 HRP Color Development Reagent, DAB
170-8238 Amplified Opti-4CN Kit
170-8235 Opti-4CN Substrate Kit
170-6432 BCIP/NBT AP Conjugate Substrate Kit
170-6412 Amplified Alkaline Phosphatase Kit
170-5012 Immun-Star™ Substrate Pack
170-5040 Immun-Star HRP Substrate, 500 ml
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22
8.8 Blotting Membranes
162-0175 Immun-Blot PVDF Membrane, 10 x 15 cm, 10 sheets
162-0232 0.2 µm Nitrocellulose/Filter Paper Sandwich, 8.5 x 13.5 cm, 20 pack
162-0233 0.2 µm Nitrocellulose/Filter Paper Sandwich, 8.5 x 13.5 cm, 50 pack
162-0234 0.45 µm Nitrocellulose/Filter Paper Sandwich, 8.5 x 13.5 cm, 20 pack
162-0235 0.45 µm Nitrocellulose/Filter Paper Sandwich, 8.5 x 13.5 cm, 50 pack
162-0236 Sequi-Blot
™
PVDF/Filter Paper Sandwich, 8.5 x 13.5 cm, 20 pack
162-0237 Sequi-Blot PVDF/Filter Paper Sandwich, 8.5 x 13.5 cm, 50 pack
8.9 Equipment
165-6001 Criterion Cell, includes tank, lid with power cables, three sample loading guides
170-4070 Criterion Blotter With Plate Electrodes
170-4071 Criterion Blotter With Wire Electrodes
Coomassie is a trademark of Imperial Chemical Industries PLC. SYPRO is a trademark of Molecular Probes, Inc. Bio-Rad is licensed to sell
SYPRO products for research use only, under US patent 5,616,502.
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