Bio-Rad cPrime Media User Manual

Nuvia™ cPrime

™

Hydrophobic

Cation

Exchange Media

Instruction

Catalog

156-3401 156-3404
156-3402 156-3405
156-3403 156-3406

numbers

Manual

Please read the instructions in this manual prior to using
Nuvia cPrime hydrophobic cation exchange media. If you
have any questions or require any further assistance, please
contact your Bio-Rad Laboratories representative.

Table of Contents

Section

Section

Section

Section

Packing Small

Packing Process Scale

Flow Properties

Buffers

Section

Section

Section

Section

Section

Section

1:

Introduction 1

2 :

Technical Description 2

3 :

Preparation

4 :

Column

Columns

5 :

Column Packing Evaluation 7

6 :

Method Development

7 :

Sanitization

8 :

Storage 11

9:

Regulatory Support 11

10: Ordering

Packing

Information

Columns

and

8

Regeneration 10

11

3

3

3

4

5

6

Section

1

Introduction

Nuvia™ cPrime™ hydrophobic cation exchange media are
designed for the process scale purification of a wide variety of
therapeutic proteins. Nuvia cPrime media’s unique selectivity
allows method developers to use hydrophobic and cation
exchange interaction modes to achieve effective purification. More
importantly, the media have a wide design space for binding and
elution, allowing for the development of highly robust methods in
a commercial manufacturing setting. Nuvia cPrime media are built
on a rigid, mechanically and chemically stable macroporus base
matrix with a particle size optimized to deliver exceptional flow
properties, fast mass transfer, and stability. See the Nuvia cPrime
media product information sheet for more product details.

If you have questions or require methods development assistance
with Nuvia cPrime media, contact your local Bio-Rad process
chromatography representative or the Bio-Rad chromatography
technical support group for assistance at 1-510-741-6563.

1

Section

2

Technical

Table 1. Nuvia™ cPrime™ media technical description.

Functional group Hydrophobic cation exchanger

Base matrix composition Macroporous highly crosslinked

Particle size 70 µm ± 10 µm

Dynamic binding capacity* ≥ 40 mg/ml

Ligand density 55–75 µeq/ml

Recommended linear flow
rate range

Pressure vs. flow
performance**

pH stability 2–14 short term

Chemical stability 1.0 N NaOH, 1.0 N HCl, 25%

Shipping solution 20% ethanol

Storage conditions 20% ethanol

Shelf life*** 5 yrs

Description

hydrophilic polymer

50–600 cm/hr

Under 2 bar @ a flow rate of 600
cm/hr

3–13 long term

HOAc, 8 M Urea, 6 M Gu-HCl,
6 M KSCN, 3 M NaCl, 1% Triton
X-100, 2% SDS + 0.25 M NaCl,
20% ethanol, 70% ethanol, 30%
isopropanol

* at 10% breakthrough hIgG.

** 20 cm X 20 cm packed bed (1.17 compression factor).
*** Stored at room temperature in 20% ethanol under accelarated
conditions.

2