Bio-Rad Bio-Rex Reactor Grade Resins User Manual
Bio-Rad Laboratories, 2000 Alfred Nobel Dr., Hercules CA 94547
LIT205 Rev B
AG®501-X8 and
Bio-Rex®MSZ 501(D)
Mixed Bed Resin
Instruction Manual
Introduction
Mixed bed resins are used for deionizing water or
other non-ionic substances such as urea, acrylamide, formamide, or glyoxal. Deionization is the complete removal
of all ionic species from a solution.
Technical Description
AG 501-X8 mixed bed resin for deionization consists
of equivalent amounts of AG 50W-X8 strong cation
exchange resin H
exchange resin OH-form. Bio-Rex MSZ 501(D) resin is
similar to AG 501-X8 resin, but the uniform bead size
minimizes pressure drops, increases resin capacity, and
allows shorter regeneration times. Molecular Biology
Grade AG 501-X8 resin is certified to be endo- and
exonuclease-free and to contain no ligase inhibitors.
Biotechnology Grade AG 501-X8 resin is certified to contain less than 100 micro-organisms per gram. AG 501X8(D) resin and Bio-Rex MSZ 501(D) resin have a blue
dye irreversibly bound to the anion exchange resin, which
turns from blue to gold when the exchange capacity is
exhausted. The resin specifications are given in Table 1.
+
form and AG 1-X8 strong anion
1
Table 1. Mixed Bed Resin Specifications
AG 501-X8 AG 501 -X8 (D) MSZ 501(D)
Resin Resin Resin
+
Chemical H
form
Resin type Mixed bed Mixed bed Mixed bed
Physical form Light and dark Blue and gold Blue and brown
Minimum total 1.5 meq/ml 1.5 meq/ml 1.5 meq/ml
capacity
Particle 300-1,180 µm 300-1,180 µm 600±100 µm
diameter
Mesh size 20-50 20-50 25-35
Matrix Styrene divinyl- Styrene divinyl- Styrene divinyl-
-
&OH
gold beads beads beads
benzene benzene benzene
H+&OH
Bio-Rex
-
H+&OH
-
All of the mixed bed resins are used for deionization.
Deionization can be performed by exchanging the solute
cations for hydrogen on the resin and the solute anions for
hydroxyl on the resin. The resulting neutralization yields
water.
Resin-H + Resin-OH + NaCl → Resin-Na + Resin-Cl + H2O
Instructions for Use
The batch method or the column method may be used
to deionize a sample with mixed bed resin.
Batch Method
The batch method is the addition of resin directly into
the sample followed by stirring to achieve deionization.
1. Weigh out about 5 g of fresh resin for every 100 ml of
sample.
2. Add resin to sample and stir or shake for 1 hour.
3. Filter or decant sample from resin.
Note: If samples contain sugars or polyhydric alcohols, it
is important to use the minimum quantity of resin required
for deionization, and to remove the resin from the sample
after the deionization process to minimize adsorption of
the sample. If the sample contains extremely hydrophilic
proteins, the resin should be placed in dialysis tubing to
avoid direct contact of the resin with the sample. If the
anion resin beads change from blue to gold prior to deionization, the quantity of resin should be increased.
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