Bio-Rad Bio-Plex Pro Wash Stations User Manual
Instructions for Use for
Bio-Plex Pro™
& Bio-Plex Pro II
Wash Stations
Revision B
Attention!
Use only the magnetic carrier which arrived with your instrument.
See page 17 for more details.
ii Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Notice
Every effort has been made to avoid errors in text and diagrams; however,
Bio-Rad Laboratories assumes no responsibility for any errors that may appea r in this
publication.
Bio-Rad reserves the right to change specifications at any time with appropriate
validation, verification, and approvals.
Copyright Information
The contents of this document are the property of Bio-Rad Laboratories and are not
to be copied, reproduced, or transferred to another person or persons without written
permission.
Copyright ©2008 Bio-Rad Laboratories
All rights reserved.
Declaration of Conformity
See page 79.
About the Instructions for Use (IFU)
This document describes the use of the Bio-Plex Wash Stations, designed for
performing hands-free wash programs on Bio-Plex and other xMAP assays.
It contains instruction for the use of the instrument and is intended as a reference for
the user. It contains information about the following:
• Installing the instrument
• Operating the instrument
• Programming of wash programs
• Definition of wash parameters
• Cleaning and maintenance procedures
Trademarks
The following product names and any registered or unregistered trademarks
mentioned in this document are used for identification purposes only and remain the
exclusive property of their respective owners:
• Microcide is a registered trademark of Global Biotechnologies Inc., Portland,
Maine, USA
• MagPlex and xMAP are trademarks of Luminex Corporation, Austin, TX, USA
• Tween is a registered trademark of ICI Americas, Inc., Wilmington, DE, USA
• Decon and Neutracon are registered trademarks of Decon Laborat ories Limited,
Sussex, UK
• HydroControl is a trademark of Tecan Group Ltd, Männedorf, Switzerland
Bio-Plex Pro and Bio-Plex Pro II Wash Stations iii
iv Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Table of Contents
TABLE OF CONTENTS..........................................................................................................................................V
BASIC OPERATION AND MAINTENANCE ................................................................................................... 11
INSTRUMENT OPTIONS..................................................................................................................................... 11
MICROPLATE REQUIREMENTS ..........................................................................................................................12
INSTALLING THE INSTRUMENT........................................................................................................................... 13
Bio-Plex Pro Installation Diagram.............................................................................................................. 14
Bio-Plex Pro II Installation Diagram........................................................................................................... 15
Installing the Tubing Bracket (Bio-Plex Pro II only).................................................................................. 16
Selecting and Installing a Plate Carrier..................................................................................................... 17
Changing the Vacuum Configuration ........................................................................................................ 18
INSTALLING THE MICROPLATE .......................................................................................................................... 19
TURNING ON THE POWER................................................................................................................................. 19
KEYPAD BASICS.............................................................................................................................................. 20
Primary Menu............................................................................................................................................ 20
Secondary Menu ....................................................................................................................................... 20
PRIMING THE INSTRUMENT............................................................................................................................... 20
Failure to Prime......................................................................................................................................... 21
PLATE WASHING PROGRAMS........................................................................................................................... 22
WHICH WASH PROGRAM DO I USE?................................................................................................................. 23
Quick Guide............................................................................................................................................... 23
RINSING.......................................................................................................................................................... 24
RINSING.......................................................................................................................................................... 25
Rinse Day.................................................................................................................................................. 25
Rinse Night................................................................................................................................................ 26
Instrument state at the end of the wash.................................................................................................... 26
CHANGING THE PLATE CARRIER....................................................................................................................... 27
PREVENTIVE MAINTENANCE PLAN .................................................................................................................... 28
Daily........................................................................................................................................................... 28
Weekly....................................................................................................................................................... 28
Every Six Months....................................................................................................................................... 29
As Needed................................................................................................................................................. 29
Yearly (Service Engineer Required).......................................................................................................... 29
Bio-Plex Pro and Bio-Plex Pro II Wash Stations v
REFERENCE SECTION .................................................................................................................................. 30
PURPOSE OF REFERENCE SECTION .................................................................................................................. 30
INSTRUMENT DETAILS.................................................................................................................................. 31
INSTRUMENT SAFETY....................................................................................................................................... 31
INTENDED USE................................................................................................................................................ 32
CONFIGURATIONS............................................................................................................................................ 33
INSTRUMENT SPECIFICATIONS ......................................................................................................................... 34
DESCRIPTION.................................................................................................................................................. 36
BIO-PLEX PRO DIMENSIONS ............................................................................................................................ 37
BIO-PLEX PRO II DIMENSIONS.......................................................................................................................... 38
REAR PANEL CONNECTIONS ............................................................................................................................ 38
REAR PANEL CONNECTIONS ............................................................................................................................ 39
DETAILED INSTALLATION ............................................................................................................................ 40
REQUIRED WORKING AREA.............................................................................................................................. 40
POWER REQUIREMENTS .................................................................................................................................. 40
UNPACKING AND INSPECTION CHECKLISTS ....................................................................................................... 40
DETAILED OPERATING INSTRUCTIONS..................................................................................................... 42
SWITCHING THE INSTRUMENT ON..................................................................................................................... 42
COMPLETE INSTRUMENT KEYPAD FUNCTIONS................................................................................................... 43
Alphabetical list of LCD text with explanations.......................................................................................... 45
DEFINING CUSTOM WASH PROGRAMS.............................................................................................................. 48
WASH MODES................................................................................................................................................. 48
Washing Positions..................................................................................................................................... 49
ASPIRATING MODES ........................................................................................................................................ 50
Normal AspirationMode............................................................................................................................. 50
Crosswise Aspiration Mode....................................................................................................................... 50
DRIP MODE..................................................................................................................................................... 51
DEFINING NEW PROGRAMS......................................................................................................................... 53
INTRODUCTION................................................................................................................................................ 53
PROGRAM MENU............................................................................................................................................. 53
DEFINE/EDIT A PROGRAM ................................................................................................................................54
Process Step: Aspirate.............................................................................................................................. 55
Process Step: Dispense............................................................................................................................ 56
Process Step: Wash.................................................................................................................................. 58
Process Step: Soak................................................................................................................................... 59
Process Step: User Prompt....................................................................................................................... 59
Process Step: Vacuum Filtration............................................................................................................... 59
SHOW PROGRAM............................................................................................................................................. 60
CLEAR PROGRAM............................................................................................................................................ 61
SETTINGS MENU ............................................................................................................................................. 61
Edit Plates ................................................................................................................................................. 62
Options Submenu...................................................................................................................................... 62
Vacuum Filtration....................................................................................................................................... 62
Bubble Sensor........................................................................................................................................... 63
PROCEDURES MENU ....................................................................................................................................... 63
Rinse ......................................................................................................................................................... 63
Prime......................................................................................................................................................... 63
Empty Prime Tray...................................................................................................................................... 63
MAINTENANCE AND CLEANING .................................................................................................................. 65
CLEANING PROCEDURES ................................................................................................................................. 65
vi Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Cleaning the Cover and Display................................................................................................................ 65
Cleaning the Liquid System....................................................................................................................... 65
Cleaning the Manifold................................................................................................................................ 66
Cleaning Waste Bottles............................................................................................................................. 66
LIQUID OR FOAM SPILLS .................................................................................................................................. 67
REPLACING THE MANIFOLD.............................................................................................................................. 68
Removing the Manifold.............................................................................................................................. 69
Installing the Manifold................................................................................................................................ 70
INSTRUMENT DISINFECTION ............................................................................................................................. 71
Disinfection Solutions................................................................................................................................ 71
Disinfection Procedure.............................................................................................................................. 72
DISPOSAL OF INSTRUMENT .............................................................................................................................. 73
Disposal of Operating Material..................................................................................................................73
Disposal of the Instrument......................................................................................................................... 73
TROUBLESHOOTING AND ERROR MESSAGES......................................................................................... 75
ERRORS WITHOUT MESSAGES......................................................................................................................... 75
ERROR MESSAGES.......................................................................................................................................... 75
Plate Error ................................................................................................................................................. 75
Program Too Big Error.............................................................................................................................. 75
Transport Error.......................................................................................................................................... 75
Manifold Arm Error .................................................................................................................................... 76
Sensor Defect Error................................................................................................................................... 76
RS485 Error............................................................................................................................................... 76
PC (Process Control) System Error .......................................................................................................... 77
No Aspiration at Vacuum Filtration Error................................................................................................... 77
LLD Errors................................................................................................................................................. 78
DECLARATION OF CONFORMITY................................................................................................................ 79
INDEX............................................................................................................................................................... 80
Symbols
Indicates the possible presence of biologically hazardous material.
Indicates the possible presence of a strong magnetic field.
Warnings, Cautions, and Notes
The following types of notices are used in this publication to highlight important
information or to warn the user of a potentially dangerous situation:
Information
Gives helpful information.
Caution
Bio-Plex Pro and Bio-Plex Pro II Wash Stations vii
Indicates a possibility of instrument damage or data loss if instructions are not followed.
WARNING
Indicates the possibility of severe personal injury, loss of life, or equipment damage if the
instructions are not followed.
WARNING
This symbol Indicates the possible presence of biologically hazardous material. Proper
laboratory safety precautions must be observed.
Attention
Negative environmental impacts associated with the treatment of waste.
z Do not treat electrical and electronic equipment as unsorted municipal waste
z Collect waste electrical and electronic equipment separately
WARNING
Risk of fire and explosion!
Ethanol is flammable and when improperly handled can lead to explosions. Proper
laboratory safety precautions must be observed.
viii Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Abbreviations
A Ampere
°C Degrees Celsius
CE Conformité Européenne
cm Centimeter
CV Coefficient of Variation
ELISA Enzyme-Linked ImmunoSorbent Assay
EN European Norm: a voluntary European standard of the European Committee for
°F Degrees Fahrenheit
IEC International Electrotechnical Commission
IFU Instructions for Use
in. Inch
IVD In Vitro Diagnostics
IVD-D In Vitro Diagnostics Directive
kg Kilogram
l; L Liter
m Meter
mBar Millibar
ml Milliliter
mm Millimeter
mS Millisiemens
µl Microliter
PC Process Control
PCR Polymerase Chain Reaction
ppm Parts Per Million
QC Quality Control
REF Reference Numbe r/ Order Number
s Second
SN Serial Number
T Träge (Slow Blow Fuse)
torr Torr – Millimeter of Mercury (mmHg)
TYPE Name and Type of instrument
USB Universal Serial Bus
V Volt
VA Volt Ampere
WEEE Waste Electrical and Electronic Equipment
Standardization or Comité Européen de Normalisation (CEN)
Bio-Plex Pro and Bio-Plex Pro II Wash Stations ix
Basic Operation and Maintenance
10 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Basic Operation and Maintenance
This section gives you instructions to configure, install, prime, and use the Bio-Plex
Pro™ and Bio-Plex Pro II Wash Stations. You can perform your first plate wash
simply by using these instructions and the onboard wash program. This section also
contains references to more detailed information throughout this manual.
Instrument Options
The Bio-Plex Pro and Bio-Plex Pro II Wash Stations are designed for strip-wise
washing of 96-well microplates. They are designed primarily for the processing of
Bio-Plex and other xMAP assays.
The wash station is available in two versions.
Wash Configuration Bio-Plex Pro Bio-Plex Pro II
Vacuum filtration No Yes
Magnetic separation Yes Yes
Both wash stations are delivered with the standard carrier installed and the
appropriate programs for performing your assays. Replace the standard carrier with
the appropriate carrier for your assays as outlined on page 17.
Which Wash Program Do I Use? On page 23 describes the standard wash programs
necessary for the different assays.
To run standard ELISAs or other plate-based assays, you must modify programs and
plate definitions, using the HydroControl™ software available on the CD that ships
with both instruments. This software is not needed for Bio-Plex or Bio-Plex Pro assay
processing. Follow the instructions in Defining New Programs on page 53.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 11
Basic Operation and Maintenance
Microplate Requirements
Use recommended microplates. If you use different microplates, your
assay results may not be reproducible, and you may experience
excessive bead loss.
Recommended microplates for magnetic assays:
Bio-Rad Part # Description Default for Magnetic Products
171-025001
*For a complete list of suitable plates, please contact Bio-Rad technical support.
Only these recommended filter plates should be used with the Bio-Plex Pro II, when configured for standard
Bio-Plex or other polystyrene bead based xMAP assays.
Bio-Plex Pro
flat-bottom plates
Yes
Recommended microplates for vacuum filtration:
Vendor Manufacturer Part # Default for Vacuum
Products
Millipore MSBVS1210 Yes
If using other microplates, you will have to enter new microplate definitions, using the
Hydrocontrol software included on the CD. Please note the following requirements.
Parameters Characteristics
Max. overall microplate height 14.35 mm ± 0.76 mm
(0.5650 inches ± 0.0299 inches)
Footprint 9.0 mm (0.3543 inches)
Pitch size (center to center) 9.0 mm (0.3543 inches)
Bottom shape Round, V-shaped, and flat
12 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Installing the Instrument
Unpack the instrument and install the instrument according to the instructions below.
Additional helpful information is located on the following pages:
• Unpacking checklist on page 40
• Bio-Plex Pro installation diagram on page 14
• Bio-Plex Pro II installation diagram on page 15
Caution
Do NOT turn the instrument on until you have completed the
steps below.
Caution
Before the instrument is installed and switched on, it should be left to
stand for at least three hours, so there is no possibility of condensation
causing a short circuit.
1. Ensure that the on/off switch in the rear panel of the instrument is in the off
position.
2. Connect the solution tubes (L1 and L2) to the inlet connectors on the rear
panel of the instrument. See the diagrams on the following pages.
3. Connect the solution tubes to the corresponding liquid bottles.
4. Connect the waste tube to the waste bottle (do not kink the tubes).
5. IF your instrument is a Bio-Plex Pro II, install the tubing bracket as shown on
page 16.
6. Fill Liquid 1 bottle with Bio-Plex wash buffer.
7. Fill Liquid 2 bottle with distilled or higher grade water.
8. Remove the standard ELISA carrier and install the appropriate plate carrier
based on the assays you will be running (Selecting and Installing a Plate
Carrier on page 17).
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 13
Basic Operation and Maintenance
Bio-Plex Pro Installation Diagram
14 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Bio-Plex Pro II Installation Diagram
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 15
Basic Operation and Maintenance
Installing the Tubing Bracket (Bio-Plex Pro II only)
Your Bio-Plex Pro II is shipped with the liquid hoses attached. Attach the tubing
bracket to the left side of the instrument, as shown in the pictures below.
Location of Tubing Bracket
A tool is provided for the installation of the bracket. It is located in a bag containing a
USB cable and cleaning pins for the manifold.
16 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Selecting and Installing a Plate Carrier
Attention!
Use only the type
of magnetic carrier
which arrived with
your instrument.
If your wash
station arrived with
version B use of
version A on your
wash station will
result in poor
assays results.
Version B is backcompatible with
previously installed
wash stations.
Magnetic Plate Carrier
(Version B)
(Version A)
Use magnetic plate carrier with: Use vacuum plate carrier with:
• Bio-Plex Pro assays • Bio-Plex assays (non magnetic)
Vacuum Plate Carrier
• Bio-Plex Precision Pro™ assays • Any assay on Bio-Plex COOH beads
• Any assay with MagPlex™
microspheres
Remove the standard ELISA plate.
1. Make sure the instrument is turned off.
2. Lift the manifold arm.
3. Slightly tilt the plate carrier towards the right.
4. Carefully slide the plate carrier toward the front of the instrument until it clears the guide bar.
Prime Tray
Guide Bar
Installing the plate carrier is the reverse of the procedure above:
• Any assay on MicroPlex
microspheres
1. Carefully guide the plate carrier onto the guide bar in a slightly tilted position, as shown
above.
2. Push the plate carrier completely into the instrument, so that the front of the carrier is even
with or past the Bio-Rad logo.
3. Lower the plate carrier.
4. Lower the manifold arm.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 17
Basic Operation and Maintenance
Changing the Vacuum Configuration
You must set the vacuum system appropriately for the plate carrier used. This is
done through the VACUUM FILTR menu. Follow the procedure shown below to:
• Enable Low VAC when using the vacuum carrier
• Disable Low VAC when
1). using the magnetic carrier
2). using the standard ELISA plate carrier
Disabling/Enabling the Vacuum Carrier
Go through these menus to disable or enable the vacuum carrier. If you are disabling
the vacuum, the menus will display “Disable” in place of “Enable.”
SETTINGS
< > OK
Navigate to Settings in the Primary menu
and press OK
OPTIONS
- + EXIT OK
Select and press OK for OPTIONS
VACUUM FILTR.
- + EXIT OK
Select and press OK for VACUUM FILTR.
Enable Low VAC
EXIT YES
Enable vacuum carrier by choosing YES
(“Disable Low VAC” will be displayed if already enabled)
Enable Low VAC
System enables vacuum carrier
EDIT LLD CHANNEL
- + EXIT OK
System places you in the Options menu at EDIT
LLD CHANNEL. EXIT to Primary menu (press
EXIT until it is no longer an option)
PROGRAM
< > OK
The instrument must be turned off and then back on for the change to take effect.
Failure to set
Vacuum Filtration
settings
appropriately for the
installed plate
carrier will result in
a MANIFOLD HEAD
UP error. This
indicates that the
manifold arm cannot
lower appropriately.
18 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Installing the Microplate
Insert the 96-well microplate into the plate transport for washing and ensure that the
microplate is correctly oriented (position A1 of the plate corresponds to position A1
marked on the plate transport). If using the vacuum plate carrier for polystyrenebased assays, see Changing the Plate Carrier on page 23.
Turning on the Power
The power switch is located at the rear of the instrument, above the power cable
connection. Make sure the instrument is plugged in using the provided po wer cable
before you attempt to operate.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 19
Basic Operation and Maintenance
F
Keypad Basics
All the functions you need to run Bio-Plex programs are easily accessed using the
keypad on your wash station. The system is organized into primary and secondary
menus.
Primary Menu
The instrument menu has the following options:
Primary
Options
Programs Wash programs used to process assays Start, Define/Edit, Show, Clear
Settings Rarely access ed advanced features Edit Plates, Options, Bubble Sensor
Procedures Preparation or maintenance processes Prime, Rinse, Empty Prime Tray,
Secondary Menu
If you choose OK when any of the primary menu options (Programs, Settings, and
Procedures) are visible on the LED, you will navigate to the secondary menus. You
may Select a choice from the secondary menu, or press Exit to return to the primary
menu.
LCD
Keypad
buttons
Menu
Functions Secondary Menu Options
PROGRAM
< > OK
< >
Navigate
to other
options
Select or
Exit
Vacuum Filtration
If you get confused about
where you are, press Exit,
until you no longer see Exit
as an option.
Priming the Instrument
You should prime both Channel 1 and 2 after installation and if the instrument has not
been used for an extended period of time. Priming is performed to fill the liquid
system of the instrument and to remove all air from the tubes. A priming step must
also be performed when switching to a different wash buffer.
Caution
Do not use your new instrument until you have primed both channels.
20 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Perform priming for both channels using the following procedure:
PROCEDURE
< > OK
Navigate to the Procedure in the primary
menu and press OK
PRIME
PRIME
< > OK
< > OK
Select and press OK for PRIME
PRIME SOLUTION OK?
< > YE S
Confirm rinsing solution and tube in bottle
WASTE BOTTLE OK?
< > YES
Confirm waste bottle is connected and
sufficie nt ly e mp t y
VOLUME
< > OK
Press OK if you see VOLUME. If you see
TIME, scroll down to VOLUME, then press OK
VOLUM E 30 mL
+ - OK
Press OK
CHANNEL 1
- + EX I T OK
Select CHANNEL 1 using the choice keys (< , >)
Select OK and the prime procedure starts
PROGRAM
< > OK
Instrument will perform the prime step and will
then be ready to perform wash protocol s
Repeat for channel 2.
Failure to Prime
If you have a new instrument or it has not been used for a long time the pump may
fail to draw fluid from the liquid bottles. This problem is easily solved.
1. Disconnect the L1 tube from the bottle (leaving it connected to the
instrument).
2. Hold the free end of the tube high and pipette 3 - 4 ml of deionized
water to the tube while performing the prime procedure in channel 1
as described above.
Alternately you may carefully use a syringe to inject 1 - 2 ml of water directly into the
L1 port of the instrument, reconnect the tubing, and run a prime procedure.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 21
Basic Operation and Maintenance
Plate Washing Programs
Instructions for running the preconfigured wash program are detailed below.
Instructions for setting new Wash, Dispense, and Aspirate modes into a new wash
program, and instrument rinse instructions, are found in the Defining New Programs
section on page 53.
PROGRAM
< > OK
START
< > OK
RUN1: Program X
< > EXIT OK
SSSSSSSSSSSS
+ - OK
Navigate to PROGRAM in the primary
menu and press OK.
Use the keys under the < and > symbols to access the
Program menu if it is not already displayed
Press OK
Select the required program and press
OK to continue
Choose OK.
Rows are represented by S (selected) or
N (not selected). All rows should be
selected.
It is highly recommended that all wells
are selected and the entire plate is
washed.
22 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
A
A
y)
Which Wash Program Do I Use?
In this instance. Use Wash Station Program
First wash, magnetic assay
ll other washes, magnetic assay
Prewash, vacuum assay
First wash, vacuum assay
ll other washes, vacuum assay
Third party kits which require 200 µl washes (Vacuum onl
For all protocols, it is highly recommended that you wash the entire plate. See page 18 for details.
Quick Guide
MAG x2
MAG x3
PREVAC
VAC x2
VAC x3
V 200 x2 or V 200 x3
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 23
Basic Operation and Maintenance
Important Vacuum Protocol Precautions
• Carefully place the plate so that it is flat and squarely seated between the metal frames on the
vacuum carrier before stating a vacuum protocol
• It may be necessary to apply a small amount of pressure to the plate for the first vacuum cycle of the
day
• Monitor your plate periodically to ensure complete removal of wash buffer before the next dispense
cycle
• Blot the bottom of the filter plate on a paper towel within 5 seconds after each program is completed.
If you are not sure how much time has elapsed, run a PREVAC program to clear any residual buffer
that may have wicked back into the wells before proceeding to the next step
Vacuum protocols will not work if you do not have buffer in every well.
24 Bio-Plex Pro and Bio-Plex Pro II Wash Stations
Basic Operation and Maintenance
Rinsing
The instrument should always be rinsed after use. Afterwards, the instrument can
either be left switched on, with the manifold in the rinsing solution, or switched off
after performing the correct maintenance.
Rinse Day
If the instrument will be left to stand for a short time (i.e. up to 2 hours), you may
perform Rinse Day with wash buffer so that you do not have to prime before the next
run.
Perform Rinse Day by making these menu choices:
When the rinsing procedure is complete, the following message is displayed:
RINSE DAY
STOP END
The manifold remains in the prime tray until END is pressed.
Press STOP to abort the procedure (prime tray will not be aspirated).
Navigate to PROCEDURE in the primary
menu and press OK.
Use the keys under the < and > symbols to access the
PROCEDURE menu if it is not already displayed
Select and press OK for RINSE
Select and press OK for RINSE DAY
Press OK when presented with the option to the left
If presented with TIME you can use the default settings here as well. Or navigate to
VOLUME using < and > .
Press OK when presented with the option to the left
Or use the default time settings
Select CHANNEL 1 or 2 (using the choice keys <, >)
Remember to prime channel 1 before performing the next
wash protocol if you rinse with channel 2
Instrument will perform rinse procedure.
Bio-Plex Pro and Bio-Plex Pro II Wash Stations 25
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