Binder CB User manual

CB (E2) Service Manual

state: 01/2002

created: 03/2002 / Jochen Tussinger

CB (E2) Service Manual

08:43:55	20.11.01
	W	X

	37.0	36.8	°C
TEMP			EXIT
CO2	5.0	4.9	%
O2	25.0	25.2	%
CONFIG	W	RESET MENUE	VIEW->

Version of the described Chamber :

Standard equipped CB CO2 Incubator E2 with

FPI Sensor System and

MB1 Controller

SERIAL-NO. 01-27954 à

Order-No. 9040-0012 / CB 150

Order-No. 9040-0013 / CB 210

			CB (E2) Service Manual
			state: 01/2002		created: 03/2002/ Jochen Tussinger
Contents
1	Modification levels ...................................................................................................................					3
2	Unit overview ..........................................................................................................................					4
	2.1	The Controller MB1...........................................................................................................				5
	2.2	Short description of the MB1 Controller ..............................................................................				6
3	Function ...............................................................................................................................					12
	3.1	The CO2-measuring principle...........................................................................................				12
	3.2	Function of the Heating System .......................................................................................				12
	3.3	Flow-Chart of the heating function (basis CB 150 wiring diagram)......................................				13
	3.4	Controller MB1 PIN description (Input / Output)			................................................................	14
	3.5	Function of the CO2 System ............................................................................................				15
	3.6	Flow Chart of the CO2 System (basis CB wiring ..................................................diagram)				15
	3.7	Function of the Permadry® system ..................................................................................				16
	3.8	Flow Chart of the Permadry® system (basis CB ........................................wiring diagram)				17
	3.9	Description of the Function of the Fan Control ..................................................................				17
	3.10		Sterilization Mode ........................................................................................................			18
	3.11		Hot - air sterilization .......................................................................................................			18
	3.12		Hot - air sterilization with inner chamber contaminated ..........with highly infective material			20
4	Trouble Shooting...................................................................................................................					22
5 Most common service work ....................................................................................................						25
	5.1	Changeing of the fan.......................................................................................................				26
	5.2	Take out of the electronic component board .....................................................................				28
	5.3	Opening of the rear service lid to achieve the area ....................................of the air jacket				29
	5.4	Setting of the door heating...............................................................................................				31
	5.5	CO2-Reference Measurement ..........................................................................................				32
	5.5.1		Measuring of CO 2 indirectly via the pH of the ..........................................cell medium			33
	5.5.2		Measuring of CO 2 directly via chemical indicator ...............................................tubes			34
	5.5.3		Measuring of CO 2 directly via a electronic measuring .......................................device			35
6	Calibration ............................................................................................................................					36
	6.1	Definition of calibration ....................................................................................................				36
	6.2	References for calibration................................................................................................				36
	6.3	Tolerance of the adjustment ............................................................................................				36
Calibration instructions for CO2 incubator CB with screen controller MB1 ........................................						37
Temperature / CO2 / O2 controller ................................................................................................						37
	Temperature calibration ............................................................................................................					37
	Calibration (alignment) of the temperature controller ...................................................................					38
	Reading out of the actual values: ...............................................................................................					39
	Entries:		....................................................................................................................................			39
	Result Calibration .........................................................(alignment) of the temperature controller					39
	CO2 calibration ........................................................................................................(alignment)					40
	O2 calibration ..........................................................................................................(alignment)					41
7	Maintenance .........................................................................................................................					48
8 Explosion Drawings of the CB /E2..........................................................................................						51
	8.1	CB .....................................................................................................Component Board				51
	8.2	CB .........................................................................Kettle / Sensors / Heaters / Permadry				52
	8.3	CB ........................................................................................Door / Sealing / Inner parts				53

2

CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

1 Modification levels

The CB CO2 Incubator E2 is a further development of CB CO2 Incubator E1. Especially the Display controller MB1 inside the red triangle is conspicuous.

The whole electronic is placed inside the lower part of the CB CO2 Incubator (Front Access Maintenance). Theres no I-box as at the CB CO2 Incubator E1 at the top of the chamber.

08:43:55	20.11.01
	W	X
TEMP	37.0	36.8	EXIT
			°C
CO2	5.0	4.9	%
O2	25.0	25.2	%
CONFIG	W	RESET MENUE	VIEW->

Display Controller MB1

Display controller MB1

Machine Room with all electronic parts

3

CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

2 Unit overview

(A)

(B)

(C)

(D)

(E)

(F)

(G)

(H)

(I)

(J)

FRONT

Machine room with all electronic parts (N)

(M)

(L)

(K)

A)Display controller MB1 for temperature and CO2 as well as O2 (option)

B)Connection socket for low tension supply (option)

C)CO2 sensor

D)Gas mixing head

E)PT 100 temperature probe

F)O2 sensor (option)

G)Internal socket 230V (max. 3 A) (option)

H)Shelf holder bar

I)Shelves

J)Shelf holder

K)Lower housing cover

L)Permadry® water basins

M)Inner glass door

N)Measuring access port

4

CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

2.1The Controller MB1

Display Controller MB1

	Operating Light
	08:43:55	W	X	EXIT-Button
		20.11.01	36.8 °C
	TEMP	37.0		(to leave a
			EXIT
	C2O	5.0	4.9 %
				Menue)
	O2	25.0	25.2 %
	CONFIG	W	RESET MENUE VIEW->	ON / OFF Button
				(Stand-by)
				ENTER-Button
				((to confirm a
				selection)
				Navigation buttons
				(corresponding function
				will be assigned)
				Mainswitch

CONFIG

W

RESET

MENUE

VIEW->

For the controller MB1, it is possible to show all setpoints and actual values at the same time. It is also possible to display the actual values portrary.

5

CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

2.2Short description of the MB1 Controller

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

3 Function

Incubators series CB are suited for cultivation of mammal cells under typical conditions of about 37°C. The incubator allows the set defined pH conditions by justifying NaHCO3 buffer systems of commercial cell media by keeping exact CO2-atmosphere inside. CB incubators care for high humidity inside to avoid increasing of the osmolarity caused by evaporation of the cell media. To reach this, the different functions heating, sterilization and CO2 injection have to work as a perfect team. This know-how is as Firmware inside the controller type MB1.

3.1The CO2-measuring principle

The CO2-measuring procedure of the incubator series CB is characterized by fast reaction times, as well as the highest accuracy and selectivity. The accuracy of the CO2 measuring system bases on a single-beam infrared measuring cell, which measures in differential mode, with permanently alternating transmission characteristic of its semi-conductor filter. Due to this highly developed singlebeam principle with Fabry-Perot interferometer (FPI), disturbance variables and aging phenomena in the measuring system are almost completely eliminated, so that this measuring system, in contrast to other measuring procedures, remains practically drift-free between calibrations and is absolutely selective for CO2.

The CO2–measuring cell contains a measuring section inside in which the absorption of infrared light depends on the number of CO2 -molecules in the beam path. This number of CO2 –molecules changes with the ambient pressure in relation to a constant volume. The distances between the molecules are consequently pressure-dependent. The collision frequency of the IR-beam with CO2-molecules increases therefore by increasing pressure.

For this reason, the ambient pressure must be compensated in order to correct the display reading of the CO2 -concentration in VOL.-%. This is achieved by entering the altitude of the site above the sea which is described in this manual.

3.2Function of the Heating System

The temperature measurement is realized by a PT100 temperature probe which changes his resistance at different temperatures. For example: 37°C = 114,380 Ω (see following chart).

The CB is equipped with a double PT100, one part is for the measurement inside the chamber, the other part is connected to the safety device class 3.1.

The measured value is evaluated in the controller MB1. The controller MB1 compares the set-value and the now measured value and decides to give a signal-current to the solid state relay –4K3 to activate the heating.

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

3.3Flow-Chart of the heating function (basis CB 150 wiring diagram)

The Pt100 temperature probe is equipped with 3 cables, two red and one white cable.

The white cable is connected to pin 1 at E1 – Pt100, the two red cables at pin’s 2 and 3 at E1 – Pt100. To measure the resistance disconnect all three cables from the controller an measure between the white cable and one of the red cables, do not measure between both red cables.

Ω

Measuring of the Pt100 resistance between the white cable PIN 1 and one of the two red cables PIN 2 or 3

13

				CB (E2) Service Manual
					state: 01/2002							created: 03/2002/ Jochen Tussinger
	Pt100 temperature probe (Temperature in °C / Resistance in Ω)
	T (°C)			0	1	2		3	4	5		6			7	8	9	10
	-10			96,086	96,478	96,870		97,262	97,653	98,045		98,436		98,827		99,218	99,609	100,000
	0			100,000	100,391	100,781		101,172	101,562	101,953		102,343		102,733		103,123	103,513	103,902
	10			103,902	104,292	104,681		105,071	105,460	105,849		106,238		106,627		107,016	107,404	107,793
	20			107,793	108,181	108,570		108,958	109,346	109,734		110,122		110,509		110,897	111,284	111,672
	30			111,672	112,059	112,446		112,833	113,220	113,607		113,994		114,380		114,767	115,153	115,539
	40			115,539	115,925	116,311		116,697	117,083	117,469		117,854	118,240			118,625	119,010	119,395
	50			119,395	119,780	120,165		120,550	120,934	121,319		121,703	122,087			122,471	122,855	123,239
	60			123,239	123,623	124,007		124,390	124,774	125,157		125,540	125,923			126,306	126,689	127,072
	70			127,072	127,454	127,837		128,219	128,602	128,984		129,366	129,748			130,130	130,511	130,893
	80			130,893	131,274	131,656		132,037	132,418	132,799		133,180	133,561			133,941	134,322	134,702
	90			134,702	135,083	135,463		135,843	136,223	136,603		136,982	137,362			137,741	138,121	138,500
	100			138,500	138,879	139,258		139,637	140,016	140,395		140,773	141,152			141,530	141,908	142,286

For Example: Your resistance measurement system shows you 114,380 Ω this corresponds to 37°C.

3.4 Controller MB1 PIN description (Input / Output)

BE1 BE2 BE3 BE4

		Ground TE
	E1 – Pt100	Output Phase-controll
		3A / 115-230V AC
		Heater air-duct
	E2 – Pt100	BA2 – 1A / 115-230V AC
		Door Heater
		BA3 – 1A / 115-230V AC
	E3 – Pt100	Collection Alarm
		BA1 – 3A / 115-230V AC
	E4 – 4 - 20mA
		Heater Kettle
		BA4 – 60mA / 5V DC
	E2 – Pt100	CO2 Solenoid valve
		BA5 – 60mA / 5V DC
		Safety device Cl 3.1
		BA6 – 60mA / 5V DC
		Fan Cold water bassin
	E – Safety device class 3.1	0(2)-10V / 0(4)-20mA
		Volatge output
		45mA / 24VDC
	Display connection	RS422

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

3.5Function of the CO2 System

The CO2 System measures the CO2 concentration inside the chamber. This information is supplied by the FPI Sensor Head. The Controller MB1 compares the engaged value with the now measured value and decides to open the solenoid valve of the CO2 gas inlet.

3.6Flow Chart of the CO2 System (basis CB wiring diagram)

FPI-Sensor à FPI Sensor Board à Controller MB1 à Solenoid Valve à Gas Injection Nozzle

								1	– white
								2	– grey	To A1+ / A2-
								3	– green
								4	– yellow
								5	– blue
								6	– red	To Power supply at the Transformer
								7	– brown
								8	- pink

H1+

Solenoid Valve CO2

1

-5Y1

2

H2-

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CB (E2) Service Manual

state: 01/2002

created: 03/2002/ Jochen Tussinger

3.7Function of the Permadry® system

Isotonic osmotic pressure ratios, essential for the growth of cells, are basically maintained in CO2 incubators by a maximum humidity content in the inner chamber. During this process care must be taken to ensure the best possible protection against contamination.

The patented Permadry® system guarantees a humidity performance of up to 98% relative humidity with completely dry inner walls. The principle hereof is totally easy. The double basin system consists of a large-surface warm water basin an a cold water basin as defined condensation point. The temperatures of both basins are in that way controlled that the humidification and the dehumidification are permanently balanced. The Permadry® system works completely free of disturbances or maintenance. The handling of the Permadry® system is the easiest thing and as safe as a coffee cup. The basin is easily removable and can be refilled in or at the unit at any time.

Cold air, produced by the Permadry® -fan, streams through the air-channel and cools the round cold water basin. The condensation point is fixed by this.

The Permadry® water basin must be placed correctly. There is a description „FRONT“ impressed.

Lettering „FRONT“

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