Olympus KHC Instructions Manual

MODEL

KHC

INSTRUCTIONS

Contents

I.

Standard Set 1

IT.

Specifications..·······..·..·····....··....····..··

....··..

···..·..·..·····..·....··..

······..· 1

m.

Name of Parts·················································································2

N. Optical Path

..··....··..

····....

··

··

····..·..·..·..·..··..·

·· ··

·..··..· · 2

V.

Structure

and

Assembly..··

· ·..··

·3

VI.

Method of Operation..· ·..· ·

·4

A.

SmootheningofCoarse

Adjustment

knob

B.

Light

Source

C.

Focusing

D.

Interpupillary

Distance and

Diopter

Adjustments

E.

UseofOil Immersion

Objective

F.

UseofCondenser

G.

ChangeofBulb

vll.

Optical Characteristics 6

W.

Major Troubles with Microscope, their Possible

Causes

and

Applicable Steps to

be

Taken ·.. ·

..

··7

IX.

Important Points to Remember ·

..

·8

I.

Standard Set

Main

Body:

(built-in

light

source,

stage

and

revolving

nosepiece)
Binocular Head
Objectives:

4x,

lOx,

40x,

lOOx,

Eyepiece ;BiWFlOx

Condenser
Filter
Spare Lamps ;

20W

Eyepiece

tube

cap

Dustproof

Cap

(for

projection

lens)
Cargille Oil
Vinyl cover
CertificateofInspection

IT.

Specifications

1

set

1

set

lea.

1 pr.
1 pc.
1 pc.

3 pcs.

2 pce.

1 pc.

1

btl.

1 pc.

1

copy

Height
Width

Depth

Net

weight

Head:

Binocular Head

Revolving nosepiece :
Focusing:
Coarse

abjustmemt

:

Fine

adjustment:

Condenser:

Illumination:
Stage:

Dimensions :

45

0

inclination,

rotatable

through

360

0

horizontally;

Stoppertofix

the

headatany designed

position.

Rangeofinterpupillary

distance

adjustment:

55.5-74mm.

Dioptric

adjustment

is possible.

Quadruple

indicator

for

Objectives'

position

Vertical

movement

stage

Rangeofoperation

28mm

Rangeofoperation

2mm

N.A.1.25

Variable iris-diaphragm
Rangeofvertical

movement

15mm

Pre-centered

20W

light

source is

built

inside the base.

Coaxial single-handle square mechanical

stage

Rangeofoperation:

Back-forth

30mm

Right-left

70mm

355mm

165mm
205mm
approx.

5.5kg

without

case

-1-

ill.

Name

of Parts

Eyepiece

Binocular

head

Coaxial

knob

for

slide

monipulation

Coaxial

coarse

and

fine adjustment knobs

N.

Optical Path

Objective

Stage

Condenser

Projection lens

Filler

Prism

Projection

Lens

Lamp

- 2 -

V.

Structure

and

Assembly

A.

Main Body

Head mount

Head

i~

atta('hl~d

upon

this

mount.

Arm

Slage

back-forth

movement knob

Spl'('iml'n ca"bemO\'t"d

back

and

forthin" rangll of

30mm.

Slage

right-left

movement knob

Sp('cimen

canbemO\'cd

ri..::hl

andl..hin

,)

ranlo1l'

uf

70mm.

Pre-set focusing lock

Fine adjustment knob

StalCe

canbemoved

\'erticaJly

in

minute

mo\'l'mcnt

in

il range

of

2mm.

Power source

cord

1.

Revolving

Nosepiece

Revolving nosepiece

Stage

clip

Stage

Condenser mount

Condenser locking screw

Condenser vertical movement knob

Conden~er

can

hl'

mon

..

J

uv

and

downina range of

lSmm

Projection

lens

This

is a detachable

screw-in

type lens.

This

can

be

remo\'cd when mirror is used.

Illumination switch

Push one end of the

switch

marked

with

a white

dOl.

to switchonthe illuminator.

The

quadruple

nosepiece

moves

very

lightly

and

click-stopsinthe

right

position

for

the

optical

path.

Mount

the

objectives

in such

an

order

that,

when

the

nosepiece is

turned

clockwise

during

observation,

an

objectiveofthe

next

higher

magnification

will

come

into

position.

2.

Pre-set

focus

lock

When

the

lockistightened,itwill

hinder

the

movement

of

the

stage

further

upward

from

that

point.

When

the

stageismoved

down once and again

broughtupcloser

to

the

lens,itwill

stopatthe

position

setbythe

lock.

This

will

help

prevent

the

speciment

to

crash

against

the

objective

and

further

facilitates

an

easy

focusing

for

the

observer.

3 -

3. Condenser
Insert

the

condenser

(1)

from

underneath

into

the

con-

denser

(g)

and secureitin

position

by

tightening

the

locking

screw

(3J.

B/';-':Binocular Head

CD

Dioptric

adjustment ring

Turn

this

ring to adjust

the dioptric

difference

between

the right and

left

eyes.

Eyepiece tube seal

Holdin~

these seats,

pull the

lubes

apart

or

push them

closer

(or

interpupilbry

distance

adjustmpnt.

Head

mounting ring

Tht'

headis£ixt.'d10th

..

main

booy

by

thi~

ring.

Interpupillary

dislance scale

/

Eyepiece

tube

Putinthe

eyepiece.

Clamping screw

Th~

head

is fixed· at

tll<'

desired

positionbythis

screw.

o How

to

Mount

the

Head

Place

the

head on the

receptacleatthe

upper

end

of

the

arm

and

turn

the

mounting

ringtefix

it.

VI.

Method of Operation

A. Smoothening of Coarse Abjustment Knob

This

coarse

adjustment

knob

operates

rather

heavily

However. such can be

adjustedtothe

operator's

preference. Hold

both

knobs

firmly,

turn

them

counterclo

kwise

(as

shown in

the

illustration)tomake

it

operate

lightly,

and

clockwise

heavily.

B.

Light

Source

Connect

the

cord

(from

the

base)

with

the

power

source. Press

the

switchonthe

white-

dotted

sidetoturnonthe

lamp.

- 4 -

c.

Focusing

1. Turn

the

fine

adjustment

knobtobring

the

index linetothe

middle

of

the

two

lines

(movement

range).

2. Place

the

specimen on

tne

stage

and secureitwith

the

clips.

3.

Put

the lOX

objectiveinposition.

4.

Looking

from

sideofthe

microscope,

move

the

stage

upward

carefully

by

the

coarse

adjustment

knob

until

the

specimen is

brought

very

close

to

the

tip

of

objective.

(**

Be

most

careful

nottohit

the

objective.)

5.

Looking

through

the eyepiece,

gradually

lower

the

stagetoobtain

rough focus.

6.

Turn

the

nosepiecetoset

the

desired

objectiveinposition.

Accrately

focus

with

the

fine

adjustment

knob.

7.

The specimen

maybemovedbythe

slide

manipulation

knob,

built

on

the

stage

so

that

the

particular

spottobe

observed

comes

into

the

centerofthe

view

field.

**** For convenience in the

later

operations,

tighten

up

the

pre-set

focus

lock

when

the

specimen is

roughlyinfocusatstep

5, above. (See

forV-A-2,

Pre-set

focus

lock)

D.

Inter-pupillary Distance

and

Dioptor Adjustments

1. The

interpupillary

distanceisadjusted

by

a.

Holding

both

eyepiece

tube

seats,

b.

Looking

into

the eyepieces, and

c.

Pulling

apartorpushing

closer

the

seats

until

the

view

fieldisobserved

in one.

When

the

adjustment

is made, read

the

figure

on the

scale;

this

will

make

the

adjustment

easier

from

the

next

time

on, asitcan be done

by

merely

setting

the

tUbes

at

the

given

figure.

2.

Diopterisadjusted

by

a.

Looking

through

the

right

eyepiece

with

the

right

eye,

b. Focusing on

the

specimenbythe

coarse and

fine

adjustment

knobs,

c. Then

looking

into

the

left

eyepiece

with

the

left

eye, and

d.

Turning

the

diopter

ring

until

the

specimen is

brought

into

focus

correctly.

When

looking

into

the

left

eyepiece

with

the

left

eye,donot

touch

the

coarseorfine

adjustment

knob.

E.

Use

of

Oil

Immersion Objective

First,

bring

the

specimen

into

focus

withalow

magnification

objective

(lOX).

Apply

a

dropofcargille

oilonthe

specimen as

wellasat

the

tipofthe

oil

immersion

objective.

Turn

the

nosepiecetobring

the

oil

immersion

objective

above

the

specimen.

This

can be

done

without

the fearofcrashing

them

into

each

other.

Focus

accurately

with

the

fine

adjustment

knob

and make

observations.

Upon

completionofobservations,

carefully

wipe

off

cargille

oilonthe

oil

immersion

objective

with

a pieceofgauze

moistened

with

xylol.

****

Cargille

oil

left

on

the

objective

can ruin the lens

greatly.

An

utmost

care

is

requiredinthis

point.

-5-

F.

Use

of Condenser

Ifanobjectiveoflow

magnification

(less

than

10X)

is in use,

lower

the

condenser

slightly

to

avoidanuneven

illumination

as

wellasforabetter

result.

The

upper-lens

of

the condenser

may

be removed. Removalofthe

upper-lens

givesabetter

result

than

loweringofthe condenser. When

the

oil-immersion

objective

is in use,

move

it

up

to

the

topofits

movement

range.

G.

Change

of

Bulb

Lay

down

the

microscope,

and loosen

two

screwsCDunderside

the

base

withacoin

or

something

like

that.

Remove the

cover

and change the used

lamp

with

a new one.

vI.

Optical Characteristics

Objective

Magnification

I

4x

1

10x

1

40x

I

100x

"",

".

Numerical

I

0.1

1

0.25

I

0.65

1

1.3

'"

aperture

(N.

A.)

'"

Working

distance

I

19.771

I

I

"

,

5.1

0.4

0.11

"'"

(W.

D.)

Eyepiece

"'.

Focal

length

I

29.2

i

15.7

I

4.3

I

1.8

""

Total

magnification

I

40x

I

100x

I

400x

11'OOOX

WF

10x

(Number

Depthoffocus

I

112.5

I

18.0

I

2.0

I

0.6

of

viewfield

18)

I

Actual

viewfield

I

4.5

I

1.8

I

0.45

I

0.18

- 6 -

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fit!

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"0

•

OLYMPUS

OB-JECTIVE

HAS

ITS

COLOUR

BAND

THE

OLYMPUS

OBJECTIVE,

you

just

purchased,

has

a

colour

band

on

it.

This

new

step

has

been

adopted

for

your

convenience,

that

you

may

unders-

tand

the

magnification,

if

you

only

see

the

colour,

without

looking

at

the

magni

ficat

ion

number

engraved

on

the

tube.

Though

there

is

no

ment

ion

about

the

colour

band in

the

instruction

booklet,

upon

your

recogni

t ion

of

our

new

colour

system,

the

olympus

products

wait

for

your

further

efficient

usage.

1=

!/&J

JfI

/'I'i:

~

If]

l-a'

,t;

~

lffi

~

Biological

use

Metallurgical

use

Range

of

Colour

Plan

Fl

Ach

Plan

Ach

magni

ficat

ion

~

Purple

1.3X

1.3X

0

-Less

than

2X

~

Brown

2X

2X 2X

-Less

than

4X

,n;

Red

4X

4X

5X

5X

4 X

-Less

than

7X

6X

.m

Orange

IOX IOX IOX

IOX

7X

-Less

than

20X

1l.

Yellow

20X

20X

20X

20X

20X

-Less

than

40X

'1t~

Brilliant

green

40X 40X 40X 40X

40X

40X

-Less

than

60X

,~.f,1c

Cobalt

blue

60X

60X

-

Less

thanlOOX

ik~j'

Light

blue

lOO

X

lOOXlOOXlOO

X

lOO

X

lOOX. and

over

is*

Colour band

OLYMPUS OPTICAL

CO..LTD.

VIII.

Major troubles with microscope, their

possible

causes

and

applicable steps to

be

taken.

Troubles

I

Possible causes

1

Stepstobe taken

Slipping

downofthe

Loosened coarse

See

SmootheningofCoarse

stage

by

itself

adjustment

knob

Adjustment

knob

and

pro-

perly

adjust

it.

Coarse

Adjustment

1.

AxisofCoarse

Adjust-

1.

Take

reverse

steps

of

knob

too

heavy

ment

knob

overly

the

above.

tightened

2.

Hardened greaseonthe

2.

Notify

the

sliding

surface.

manufacturer.

-

Fine

Adjustment

knob

Hardened grease on

the

Notify

the

manufacturer.

too

heavy

sliding

surface

Dust/Oirt

on

objective

1.

Eyepiece

tubes

are

left

Wipe

off

the

top

lensofthe

without

eyepieces

or

objective

with

clean gauze;

cap.

do

not

touch

inner lenses

2.

Fingerprints

leftonthe

but

notify

the

manufacturer

lens;

Lens

hitting

the

speci-

men;

or

cargille

oil

left

on

the

oil-Immersion

lens.

Dust/Dirt

on eyepiece

1.

Careless

storage.

1.

Be

suretostore

the

eyepieces

after

use in

the

provided

container

or

in a

desiccator

2.

Fingerprints

left

on

the

2.

Wipe

off

with

clean

lens

surfece

gauze. Do

not

try

to

disassemble

the

eyepiece. Any change in

arrangement

of

lenses

can

deteriorate

the

entire

opical

efficiency.

Scratches on

the

lens Lens

was

wiped

with

a piece

Immediately

notify

the

of

clothorleather

while

manufacturer.

The lens

will

dustorfilthisstill

left

on.

be replaced

at

the

actual

cost.

- 7 -

IX. Important points to remember

Dampness and

dust

is a

taboo

for

the

microscope. But,

frequently,

a lab where

the

microscope

is used is

not

freeofsuch.Itis the besttostore

microscope

after

each use

in

the

provided

container.Iffrequent

observations

make

it

impossible,

at

least

cover

the

instrument

with

the

provided

vinyl

cover.

The

objectives

and eyepieces should best be

stored

in a

desiccator.

Also recommended

is

to

place a packofsilicagel

in the container.

After

the eyepiece is removed

from

the

instrument,

be suretocover

the

eyepiece

tube

with

the

provided

cap. Never

attempt

to

disassemble or

repair

the mechanical

partsofthe

microscope.Itmust

be done

by

specialists.

Cleaning

must

be

performed

with

utmost

care. For expample,

dust

off

with

a

soft

brush or

blow

off

byarubber

ball where hands cannot reach.

Mirror

(optional

accessory)

In

general,

microscopy

with

KHCisconducted

with

the

projection

lens on. However,

for

special

illumination

effect

this

mirror

can be used

for

high

magnification

lens. and

the

other

side, concave surface, is good

for

observation

with

low

magnification

objl'ctives.

- 8 -