Olympus IX-FLA User manual

OLYMPUS

IX-FLA

INVERTED

OBSERVATION

REFLECTED

INSTRUCTIONS

LIGHT

FLUORESCENCE

ATTACHMENT

This

instruction

Observation

with

the

before

use

operating

manual

Attachment.

of

this

attachment,

the

attachment

is

To

for

obtain

the

Olympus

optimum

we

recommend

IX-FLA

Inverted

performance

that

and

you

Reflected

to

familiarize

study

this

Light

Fluorescence

yourself

manual

thoroughly

fully

AX5918

IMPORTANT

This

unit

employs a UIS

UIS

microscope

if

inappropriate

a

Getting

This

attachment

Operating

N=

Installation

.

The

Make
Control
The

σσβω

to

s

Do
parts

Do
The

.

To

Before
and

minutes
‘The
allow

.

When

and

temperature

category

high

pressure

sure

that

ultraviolet

use

the

not

open

will

be

not

apply

power

avoid potential

opening

unplug

or

temperature

ample

installing

surrounding

frames,

accessories

Ready

is a precision

and

(overvoltage

mercury

that

the

power

the

burner

is

rays

UV

protective

the

lamp

extremely

excessive

supply

the

the

lamp housing's

more

free

the

walls

installed

emitted

shield

housing

hot

force

unit

contains

shock

hazard,

lamp

housing

until

the

the

vicinity

space

around

power

to

lamp

prevent

in

supply

(universal

eyepieces,

instrument.

relative

burner

supply

infinity

condensers,

are

used.

humidity

category)

I

(mercury

unit's

main switch

correctly

by the

burner

are

with

the

while

and

to

unit.

the

burner

will

cause

the

stop

high

be

mechanisms

voltage

sure

to

for

replacement

connecting

housing

the

lamp

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in

particular

unit

(BH2-RFL-T3),

cools

of

overheating.

system)

etc.

Handle

it

with care

should

be

in

(in

accordance

arc

lamp)

and

that

harmful

(See

is

thermal

the

should

is

set

all

cords

and

should

page

10.)

turned

on

injury

if

that

components.

ground

the

power

of

the

plug

from

burner

cord

down.

housing

will

greatly

above

the

ensure

optical

design, and

Less

than

and

avoid

range

of

with

0°-40°С

IEC664).

Pollution

be a USH102D

to O (OFF).

are

or

for

touched,

all

the

Never

cord

the

output

lamp

that

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correctly

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be

directly

at

least

(See

functions

attempt

wire.

or

other

connector

increase.

housing

there

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optimum

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performance

it

to

(32°-104°F)

degree

(mfd,

not

10

page

to

internal

When

more

by

so,

set

connected.

minutes

are

the

looked

at

after

12)
provided

disassemble

parts, set the

on the

installing the

than 5 cm

be

used

sudden

or

and

30-90%,

II

(in

accordance

Ushio

Electric)

main

switch

with

unprotected

it

is

turned

with.

the

unit

main

power

supply

microscope,

free

space

only

with

may

result

severe

impact.

respectively.

with

to O (OFF)

eyes.

off.

Lamp

switch

to © (OFF)

unit,

Wait

make

between

IEC664)

Be

sure

housing

for

10

sure

to

its

sides

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a

Maintenance

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Be

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clean
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potential
any

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3

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4

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5.

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Caution

If

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safety

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in

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wiping

or a mixture

ether

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of

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components

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disassemble

mercury

burner

the

not

equipment

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bumer

with a new

using

the

mirror

cube

is

equipment

symbols

following

|

symbols

safest

possible

Indicates

Before

ΡΕ]

Indicates

Storage

dirt

or

with a piece

ether

alcohol

of

(especially

any

attachment,

used

are

fingerprints

of

gauze.

(70%)

and

are

electrical

alcohol

highly

sparks,

plastic

part

of

the

attachment.

life

of

200

one.

(See

keep

is

not

in a manner

may

also

found

it

going

to

not

be

damaged.

on the

microscope.

manner.

that

the

surface

use,

carefully

read

that

the

main switch ON.

on the

lenses,

To

remove

(30%).

flammable,

such

as

parts).

page

covered

be

main

To

clean,

hours.

When

10.)

with

used

for a while,

specified

Always

becomes

the

instruction

fiters,

or

fingerprints

be

careful

switches.

the

or

oil

to

keep

Never

use a lint-free, soft

the

hour

counter

the

provided

dust

place

it

in

by

this

manual,

used

Study

the

the

the

equipment

meaning

Explanation

hot,

and

should

not

manual.

high

pressure

stains,

wipe

with

these

chemicals

attempt

to

cloth

on the

power

cover

and

its

safety

of

the

be

store

container

of

the

as

outlined

symbols,

touched

mercury

burner.

gauze

slightly

away

use

organic

lightly

moistened

supply

unit

it

at a dry,

and

store

it

in a safe

equipment

in

this

instruction

and

always

with

bare

hands.

If

contaminated,

moistened

from

open

solvents

with

fire

and

to

clean

with a diluted

indicates

200

hours,

clean

place.

place.

may

be

impaired.

manual

use

the

microscope

in

Indicates

ο-

that

the

main switch

OFF.

om

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dsev

3-1

Module

3-2

Detailed

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sine

conteos...

observaron

5-1

Summary

5-2

Simultaneous

Light

5-3

Simultaneous

Light

“TROUBLESHOOTING

System

Assembly

rue

Phase

Differential

Chart

of

Observation

Reflected

Contrast

Reflected

Interference

Procedure

Procedures

Light

Fluorescence

Observation

Light

Fluorescence

Contrast

GUIDE

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and

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and

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Observation.....

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9

ONTROLS

@

Fluorescence

(IX-RFAC)

Mirror

Cube

IX-FLA

Housing

©

Mirror

Cube

Turret

Index

sticker

à

—

*

Consists
dichroic
Do

not

À

Index

plate

of a combination
mirror

to

suit

the

disassemble

the

mirror

>=

Shutter

knob

=

position

of

barrier

filter,

excitation

method.

cube.

exciter

filter,

and

=

3
2

은

=

Field

iris

@

Fluorescence
(IX-RFA/S)

Filter

slider

apertureffilter

Vertical

shutter/empty

mount

diaphragm

lever

Illuminator

diaphragm

centering

knobs

©

Mercury

Burner

Lamp

Housing

5

CONTROLS

Collector

ollector

(IX-UCLHG/XEB)

Lamp

Socket

(U-ULS100HG)

Li

Lens

Unit

Lamp

i

Collector
focusing

lens

knob

Housing

(U-ULH)

@

BURNER
LED

indicator

Hour

counter

Power

ON

Supply

Unit

AE

receptacle

Connector

©

For

assembly

*

When

assembling,

touching

ho

ASI

glass

of

the

IX50

make

surfaces.

or

IX70

microscope,

sure

that

=

see

all

parts

are

замов

32ΝΟΙ2().
32ND25
32ND50

diaphragm

iris

Field

knobs

centering

1010

iris

diaphragm

the

instruction

free

of

dust

Extension
of

(UCLA)

()

()

()

\

verd

Cube

manual

provided

and

dirt,

and

handle

the

collector

Fluorescence

vertical

(XRFAS

for

lens

>

Collector

(IX-UCLHG/XEB)

Muminator

jes

with

avoid

adjustment

unit

Lamp

ss

the

microscope.

scratching

housing

ULH)

any

Ноно

unit

lens

parts

or

A

ATSNISSV

©

Refer

different

i

1x501x70

to

page

17

observation

for

details

methods.

Centering

screen
(U-CST)

on

objectives

cS

=>

cube

UX-RFAC)

target

compatible

housing

with

mirror

=

Power

supply

(BH2-RFL-T3)

3

unit

Allen

screwdriver

the

IX

microscope

Allen

wrench

fluorescence

Power

cord

(UYCP15)

(provided

frame.)

(provided

vertical

with

illuminator.)

with

3

ASSEMBLY

@]

Mounting

©

Refer

to

observation

1.

Remove
in

place

*

After
the

dichroic

the

cover.

©

Dummy

you

wish

When

using

mount

2.

Rotate

the

at

the

position

3.

Using

the

the

cube

toward

4.

Hold

the

it

all

the

screw

©.

5.

Remove

responding

*Use a sharp

to

lift

the

*

The

and

*

When

dummy

observation

However,

dummy

protruding
dummy

6.

Put

back

the

page

18,

section 9 for

methods.

the

cover @ from
magneticaliy.
assembly,

mirror

plates @ are

to

use

only

two

or

the

actual

mirror

of

Allen

screwdriver

clamping

the

perimeter

cube

to

be

way

onto

the

cube’s

turret

object

magnetic

alphabetic

those

at

the

performing

plates

only,

be

sure

plate

screw

plate by

the

cube

Cubes

details

the

mirror

if a cube

cube(s)

cube

the

or

exciter

mounted

one

cube,

more

cubes,

instead.

housing's

cube

is

to

in

mount

mounting

provided

screw

©.

While

of

the

turret,

mounted

the

index

such

cube

letters

turret's

in

is

housing's

with

dovetail

magnetic

fluorescence

the

heads

reversing

sticker

as

the

tip

index

on

the

index

empty

it

is

unnecessary

to

tighten

mounted.

may

index

cover

on

cubes

compatible

cube

housing

be

replaced,

filter

with

your

three

of

the

four

it

in

the

remaining

remove

the

dummy

turret @ to

sliding

remove

its

mount

place

hole.

with

the

microscope

the

it

by

index

side

©.

Then

sticker @ and

position".

of a ballpoint

sticker.

mirror

cube

housing's

positions

observation,

cube

the

If

obstruct

the

procedure

have

positions.

to

mount

cube

clamping

the

screws

turret

©.

(Figs.

with

©.

The

cover

make

sure

not

hands

when

cube

positions.

empty

platels)

the

dummy

frame,

dummy

plate @ slightiy

pulling

it

upward

facing

upward

tighten

the

affix

it

to

pen

or

mechanical

cube

no

importance

make

sure

For

transmitted

dummy

screw

are

left

loose,

rotation.

described

Mount

in

1,23)

different

is

held

to

touch

removing

When

position.

and

then

plate

@

loosen

and

slide

clamping

the

cor-

pencil

dovetail

here.

to

mount

light

plates.

where

no

the

the

steps

2-4.

IX-FLA

Fig.

5

Mounting

Cube

%

Before
make
(OFF).

1.

Using

the

microscope's

2.

Positioning

the

mirror

cube

housing

3.

Using

the

microscope's

a

Mounting

©

Light

excluding

be

attached

shape,

respectively.

1.

Attach

one

the

mirror

portion @ of

2.

Turn

the

3.

Attach

the

side.

the

Fluorescence

Housing

mounting

sure

Allen

the

cube

Allen

at

of

cube

clamping

other

(IX-RFAC)

or

that

the

screwdriver,

mirror

cube

mirror

cube

housing

compartment.

screwdriver,

mirror

cube

the

Light

plates

protect against

both

the

the

light

excluding

housing

the

groove

knob @ and tighten

light

excluding

removing

power

the

supply

loosen

housing

dovetail.

housing

dovetail @ as

as

far

as

it

will

firmly

tighten

housing

dovetail.

Excluding

left

and

right

plates

so

that

the

clamping

on

the

microscope

plate

in

Mirror

mirror

units

main

the

clamping

Remove

go

into

the

clamping

Plates

leaking

ultraviolet

side,

and

(for

right

knob @ and
frame

firmly.

the

same

(Fig.

본

cube

housing,

switch

always

is

set

screw © of

the

housing

dummy.

shown

in

Fig.

4,

insert

the

microscope's

mirror

screw @ of

(Fig.

light

and

should

each

plate

has

different

side) © provided

the

threaded

mate

together.

manner

at

the

opposite

4)

to

O

the

the

5)

with

В

ATBNISSY

Fig.

6

Mounting
Illuminator

1.

Using

the Allen

loosen

the

on

the

rear

ο

.

Insert

the
manner
holes @ of

so

Further
pin

fits

illuminator

5

Using

the

the

(IX-RFA/S)

wrench

two

screws © on

of

the

fluorescence

that

the

field

the

fluorescence

insert

the

into

the

positioning

mount

Allen

wrench,

Fluorescence

provided

microscope.

iris

fluorescence

on

the

with

the

fluorescence

vertical

diaphragm

vertical

vertical

groove © on

microscope.

tighten

Vertical

the

fluorescence

illuminator

the

into

knob

seats © fit

illuminator

illuminator

two

screws © on

vertical

vertical

illuminator

the

mount

mount.

until

the

fluorescence

6)

(Fig.

illuminator,

mount

in

such

a

into

the

mount

its

positioning

vertical

the

mount.

3

ASSEMBLY

Attaching

Knobs

1.

If a stage

with

Insert

N

on

the

slightly

“

‘Screw

fluorescence

4.

Using
diaphragm
it a little.

σι

Rotate
ease

Mount

o

G

wounting

1.

Slide the

of

the

2.

Insert
x

Before
cooled

©

Compatible

the

and

is

mounted

the

Allen

wrench

the

two

field

fluorescence

before

they

the

field

iris

vertical

the

Allen

screwdriver,

lever's

the

operation

during

observation

the

stage.

the

fluorescence

arrow.

the

filter @ into

replacing
down.

filter

sizes

Field

the

Field

to

and

iris

diaphragm

vertical

can

be

diaphragm

illuminator

operation

knob

is

ND

Filter

vertical

the

filters,

are:

Iris

Diaphragm

Iris

Diaphragm

the

microscope,

remove

centering

illuminator.

turned

lever @ into

until

turn

the

knob © slightly

©).

When a position

found,

tighten

illuminator's

U-shaped

make

Outer

diameter

loosen

the

stage.

Turn

no

further.

the

it

stops.

clamping

the

filter

filter

holder

sure

the

32

Centering

Lever

the

clamping

knobs

(7)

into

their

the

knobs

clockwise

threaded

screw © of

counterclockwise

clamping

slider © in

filter

mm;

hole

the

that

offers

operational

screw @ firmly

the

@).

and

filter

holder

thickness

max. 6 mm

(Fig.

7)

screw

seats

©

until

(4)

in

the

field

iris

to

loosen

(Fig.

8)

direction

has