Olympus IX81-ZDC2 User Manual

INSTRUCTIONS

IX81-ZDC2

FOCUS DRIFT COMPENSATING

MICROSCOPE

This instruction manual is for the Olympus Focus Drift Compensating Microscope Model IX81-ZDC2.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.

A X 7 9 0 6

Compliance

This device complies with the requirements of both directive
compatibility and directive
with the above directives.

IEC61326-1 defines two categories according to the location for use.

Class A: Equipment suitable for use in establishments other than domestic, and those directly con-

nected to a low voltage power supply network which supplies buildings used for domestic
purposes.

Class B: Equipment for use in domestic establishments, and in establishments directly connected to a

low voltage power supply network which supplies buildings used for domestic purposes.

This system is applied Class A. Some interference may occur if this system is used in domestic location.

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

2006/95/EC

concerning low voltage. The CE marking indicates compliance

2004/108/EC

concerning electromagnetic

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user‘s authority to operate the equipment.

CONTENTS

IX8 1-ZDC2

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 MODULE NOMENCLATURE

2 NOMENCLATURE

3 OPERATION

4 SPECIFICATIONS

5 TROUBLESHOOTING GUIDE

1-5

6

7

8

11

12

IMPORTANT

} The IX81-ZDC2 microscope features the capability of compensating for the focus drift that poses problems

during long hours of time-lapse observation of live cells.

The microscope has two focus drift compensation modes including the one-shot mode and continuous focus

mode, which are to be selected according to the observed specimen.

} This manual pertains only to the information related to the IX81-ZDC2 (focus drift compensation) facility of the

microscope. Please also refer to the instruction manuals for the microscope and associated modules.

} The IX81-ZDC2 can be controlled with the MetaMorph application software. For the compatible version of the

software, consult the respective company.

} For the applicable modules, contact Olympus or check the latest catalogue for this product.

Safety Precautions

1. The focus drift compensation function uses a laser diode (wavelength 785 nm) for autofocusing. It is designed to be safe
by reducing the laser power (Class 1), it is still not recommended to view the laser light directly by removing the cover, etc.

Never remove the warning and caution labels on the microscope.
The semiconductor laser for autofocusing incorporated in this unit makes it designated as a product of the following

class.

CLASS 1 LASER PRODUCT (IEC60825-1:1993+A1:1997+A2:2001)

This product complies with 21 CFR 1040.10 and 1040.11 except for deviations
pursuant to Laser Notice No.50, dated (June 24, 2007).

2. Never attempt to remove the cover using a tool. There is a risk of exposure to the internal strong laser beam as well as
other malfunction or failure.

[Semiconductor laser, wavelength 785 nm, output (max.) 85 mW, equivalent to Class 3B]

3. Always use the power cord provided by Olympus. If the proper power cord is not used, product safety performance
cannot be warranted.

4. Always ensure that the grounding terminal is properly grounded. If the equipment is not grounded, Olympus can no
longer warrant the electrical safety performance of the equipment.

5. Distribute the connection cables at a distance from the lamp housing. If a connection cable contacts the lamp housing
or its surroundings, the cable may be melted and cause n electric shock hazard.

6. Never insert metallic objects into the air vents of the microscope as this could result in electrical shock, personal injury
and equipment damage.

7. To prevent the microscope system from falling down, do not install modules which cause the microscope system height
to exceed 1 meter.

8. Do not use an objective other than those specified to be applicable (see page 4).

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment

in the safest possible manner.

Symbol Explanation

Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.

1

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

2

IX81-ZDC2

3

CAUTION

Control or adjustments using procedures other than those described in this manual may result in hazardous

exposure to the laser radiation.

Warning labels

Warning labels related to laser beam are attached to the microscope. Always heed the warnings.

Warning label position (AF sensor unit)

Left side panel

When a warning label becomes dirty or is peeled off, contact Olympus for replacement.

1 Getting Ready

1. The microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.

(For the operating conditions, refer to chapter 4, “SPECIFICATIONS” on page 11.)

3. For the objectives applicable to the microscope, see the list on page 4.

2 Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

CAUTION

Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical

equipment that is being switched on or off, which could cause ignition of a fire.

Also remember to always use it only in a well-ventilated room.

2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble or modify any part of the unit as this could result in malfunction or reduced performance.

Particularly, the stage support cannot be removed because it has been bonded after adjustment.

4. When not using the microscope system, make sure that the lamp housing is cool enough, and cover the microscope
system with the dust cover before storage.

5. Before disposing of this microscope, be sure to follow the regulations and rules of your local government.

3 Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

CAUTION

: Indicates that failure to follow the instructions in the warning could result in bodily

harm to the user and/or damage to equipment (including objects in the vicinity of the

equipment).
Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).

3

Notes on ZDC (Focus Drift Compensation)

CAUTION

When using an objective with correction collar, always adjust the collection collar before using the focus

drift compensation function. Otherwise, the focus drift compensation will not be available.

Be sure to use an anti-vibration bench to enable the focus drift compensation to function properly.

1. One-shot focus mode
In this mode, the 785 nm laser beam is first focused on the glass bottom dish’s cover glass bottom surface (or on the

cover glass top surface in the case of an oil- or water-immersion objective) and then moved mechanically from there to
the target cell using software to reproduce the desired focus position.

Although this mode requires a certain period for moving the focal point, the capability of arbitrary setting of the focus

movement range makes it suitable for multi-point time-lapse observation.

2. Continuous focus mode
In this mode, the laser beam is focused continuously on the top surface of the cover glass of the glass bottom dish at

the same time as the target cell. During this, the focus is corrected automatically even when the change in environmental
temperature due to heat cause defocusing.

The range in which continuous focusing is possible is determined by the objective power, namely 20 µm with the 40X

objective and 10 µm with the 60X objective. With the 100X objective, the range varies depending on the model of the
objective.

Although this mode enables continuous observation (at the video rate), the fixed focusing range makes it suitable for

observation in which the cell position does not vary greatly, such as the TIRF (Total Internal Reflection Fluorescence)

observation.
} Range in which continuous focusing is possible: (Only when using a glass bottom dish)
UAPON 100XO
UPSAPO 100XO 3.5µm or less
UPLFLN 100XO2 3µm or less
UPLFLN 100XOI2 3µm or less

TIRF

4µm or less

4

IX81-ZDC2

5

3. Applicable objectives:

CAUTION

APON 60X O
UAPON 100X O

PLAPON 60X O 1.42 0.15
UPLSAPO 60X O

UPLSAPO 100X O

UPLFLN 100X O2
UPLFLN 100X OI2

UPLSAPO 20X*
UPLSAPO 40X2*
UPLSAPO 60X W

UPLFLN 20X*
UPLFLN 40X*
UPLFLN 60X*

LUCPLFLN 20X*
LUCPLFLN 40X*
LUCPLFLN 60X*

UAPO 40X OI3/340 1.35-0.65 0.1

· If objective has an aperture, set it wide open.

· The AF focusing speed is approximately 1 to 5sec. in the focusing range,
but slows down depending on the AF search range setting.

· The focus drift compensation is not available with the phase contrast
objectives because these incorporate a phase plate.

· The continuous focus mode can be used only with an oil- or water-immersion
objective. If an objective other than oil- or water-immersion objective is used,
the one-shot focus mode should be used.

· When using a large specimen such as a micro-test plate, focusing may not
be achieved due to its inclination.

The focusing is guaranteed only with a glass bottom dish with a diameter

of 35 mm.

Objective Name NA WD (mm)

TIRF

TIRF

* Compatible with one-shot focus mode only.

1.49

1.49

1.35

1.40

1.30

1.30-0.60

0.75

0.95

1.20

0.50

0.75

0.90

0.45

0.60

0.70

6.60-7.80

2.70-4.00

1.50-2.20

0.1

0.1

0.15

0.13

0.20

0.20

0.60

0.18

0.28

2.10

0.51

0.20

} The previous models to the objectives listed above (the objectives having a smaller number or no number at the end of

model name) can also be used.

4. With DIC (Differential Interference Contrast) observation, focus compensation is possible only with the gray sensitive color
and in the one-shot focus mode. It is neither possible in phase contrast observation.

5. Operating environmental temperatures: 18 to 40°C (64.4 to 104°F)

(Focus compensation is possible under a temperature variation range within

5°C (41°F) and when the microscope is protected against the wind from air
conditioners.)

5

6. Restrictions on combined modules

· The module that can be mounted on the left side port is only a TV adapter that can project the primary image in 1X

magnification, that is, the U-TV1X, U-DPCAD or U-SIP. (Optimal AF performance cannot be achieved with other combination.)

· Note on stage
The IX-SVL2 stage cannot be combined with this microscope.

· Notes on observation tube

a) When the U-TBI90 observation tube is used, the image in the peripheral part of eyepiece’s visual field may be

obscured (about 5%). In addition, no intermediate attachment can be used with the U-TBI90.

b) When the U-BI90/BI90CT observation tube is used together with a 4X or 10X objective, the image in the peripheral

part of eyepiece’s visual field may be obscured. In addition, the IX2-CA2X magnification changer lens unit cannot be
used with the U-BI90/BI90CT (it becomes usable by mounting the U-EPA2 eye-point adjuster (occupying only 1
stage) on the U-BI90/U-BI90CT).

· Notes on intermediate attachments

a) Only one U-EPA2 eye-point adjuster can be mounted (it cannot be used together with other intermediate attach-

ments).
b) Only one U-FWO filter wheel can be mounted (it cannot be used together with other intermediate attachments).
c) When the IX-ATU intermediate attachment and GX-SPU side port unit are combined, the image in the peripheral part

of eyepiece’s visual field may be obscured if the magnification changer knob of the microscope frame is set to 1X.
d) The U-CA/U-ECA/U-ECA1.6X magnification changer cannot be used in combination with the microscope frame.

· When the IX-ATU intermediate attachment and U-TR30H trinocular observation tube are combined, the only TV adapter
that can be used is the U-TV1X.

· When the GX-SPU side port unit and a TV camera are used, the only TV adapter that can be used is the U-TV1X.

· The FN of the right side port is specified as 16. When a 10X or lower-power objective a CCD camera are used, the
brightness in the peripheral area may be slightly insufficient.

· When a 2X, 4X or 10X objective is used with FN 22, the brightness in the peripheral area may be slightly insufficient.
(This problem does not occur with FN 16.)

· When a 10X objective is used in transmitted light observation using the IX2-ULWCD condenser, the illumination NA
may become insufficient and the resolution may degrade. Also, optimum phase contrast observation is not available
using a phase contrast objective (PH1 or PH2).

· When using the IX2-RFAW, remove the light shield @ in the excitation
filter slider on the left illuminator to prevent it from coming in the way of
operation.

Some filter sliders are not usable with this microscope.

@

· Optimum phase contrast observation is not available when the IX2-ILL30 transmitted light illumination column and a
phase contrast observation (PH1 or PH2) are used.

· AF does not function normally when the U-LH100 transmitted light IR lamp housing is used. To achieve AF, it is required
to close the shutter or turn off the light during AF.

· The IBMU/IBML temperature retention box cannot be used because a clearance is produced between the tempera­ture retention box and transmitted light illumination column.

· When performing autofocusing in the continuous focus mode, use the provided IR slider to prevent the IR laser from
leaking into the observation light. Note that, when this slider is used, fluorescent dyes with IR-domain absorption
wavelengths (700 nm or more) can become unusable or their brightness is reduced.

When this slider is used, the fluorescence mirror unit used in the DIC observation should be the U-MDICT.

· If the I2-ZDC2PCB control board for exclusive use with IX81-ZDC2 is installed on the IX2-UCB control box, uninstall
other focus control boards.

6

IX81-ZDC2

7

1

} The IX81-ZDC2 can be combined with the same modules as the IX81 microscope systems, but certain modules are

subject to restrictions with regard to the combination with the stages, thermal incubation boxes (IBMU/IBML) and interme­diate attachments.

In addition, troubles such as “obscuring” or “insufficient peripheral brightness” may occur depending on combination of

modules (see “6. Restrictions on combined modules” on page 5).

Transmitted Light Lamp
Housing

Transmitted Illumination
Column

Reflected Light
Filter Wheel

MODULE NOMENCLATURE

Condenser

· IX2-LWUCDA2

· IX2-LWUCD

· IX-ULWCD

· IX2-DICD

· IX2-MLWCD

· U-UCD8
(Used together
with IX-ADUCD)

Stage

· IX2-SFR

· IX2-GS

· IX2-SP
(Can be used
together with
IX-MVR)

Eyepieces

· WHN10X

· WHN10X-H

Shutter

Reflected Light
Lamp Housing

Reflected Fluorescence
Illuminator

· IX2-RFA

· IX2-RFAL

· IX2-RFAW

· IX2-RFAEVA-2

· IX2-ARCEVA

Auto Focus Sensor Unit
IX2-ZDC2

Do not remove this sensor unit
since it is a part of the IX81-ZDC2
system and assembled by the
manufacturer. Do not apply ex­cessive force to the auto focus
sensor unit and do not place
anything on it.

CCD camera

Camera
adapter

IR slider

High-Intensity
Power Supply
Unit

Observation Tube

· U-BI90CT

· U-BI90

· U-TBI90

· U-TR30H-2
(Used together
with IX-ATU)

Microscope Frame

· IX81F-ZDC2

· IX81S1F-ZDC2

Fluorescence Mirror Unit
Cassette

· IX2-RFACA

· IX2-RFAC

7

2

NOMENCLATURE

} The module names enclosed in are involved in focus drift compensation.
The assembly and adjustments are to be done by Olympus.
Also when the system is going to be transported and reinstalled, consult Olympus.

Warning label

Contact Olympus if this is
peeled off.

Class 1 laser
output direction

Stage

AF sensor unit

Wavelength : 785 [nm]
Beam divergence :
@Objectives attached

0 to 1.49 [NA]
Depend on objective lens

2Objectives removed

-0.14 to 0.03 [deg]

Depend on offset lens position
Pulse duration : 2.5 [ms]
Pulse repetition rate : 100 [Hz]
Maximum power output : 127.5 [µW]

*

AF port switching unit

IR slider
Provided with the

IX81-ZDC2 system

Application software

Use a system meeting the
IEC60950 requirements.

CCD camera

Focus Handle Interface
U-IFFH

PC

Microscope Frame

AF port switching lever

Pulled-out position: OUT.
Pushed-in position: IN.

Control board

· I2-ZDC2PCB
This board can be
used only with a
ZDC2-dedicated
microscope frame
(IX81F-ZDC2 or
IX81S1F-ZDC2).

Control Box

IX2-UCB

Focus Handle
U-FH

Hand Switch

U-HSTR2

8

IX81-ZDC2

9

3

OPERATION

3-1 Operating Precautions

The Class 1 laser beam that is output from the objective is not hazardous (but direct staring for an extended period is still

inhibited), and the laser beam intensity will not increase even in the case of malfunction. However, to prevent an incident,
make sure not to tilt the glass bottom dish so that the reflected laser beam will not be scattered around.

3-2 Focus Drift Compensation Procedure

(Fig. 1)

Fig. 1

@

1 Preparation

1. Set the power switches of the IX2-UCB control box and PC to “ I ” (ON).

2. Set the AF port switching lever @ of the AF sensor unit to the pushed-in
position (IN).

} This operation engages the dichroic mirror (DM) in the light path of the

AF sensor unit, making AF detection possible.

If AF detection is not necessary, setting the lever @ to the OUT position

makes it possible to eliminate the small loss of the observation light.
This is also effective in observation with IR light (785 nm or higher
wavelengths).

2 Adjusting the Correction Collar

If you use an objective with collection collar, the following adjustment is

necessary.

1. How to perform correction for bottom thickness of the glass-bottom dish:

When the thickness of the bottom of the glass-bottom dish is known,

match the scale reading of the correction collar to the thickness of the
glass bottom.

2. How to find the optimum position based on image resolution and contrast:

· If the thickness of the glass-bottom dish is unknown, the optimum position
for the collection collar can be obtained by judging the image resolution
and contrast. When a satisfactory image is not obtained after focusing,
rotate the correction collar to the left and right, refocus each time and
compare the images at both sides. Then rotate the collar in the direction
yielding a better image, and rotate the collection collar to the left and
right, refocus each time and compare the images. Repeat this cycle until
the position with the optimum image is found.

9

1

Fig. 2

3 Using Oil- or Water-Immersion Objective

} If you use an oil-immersion objective, use immersion oil as described

below.

CAUTION

CAUTION

CAUTION

Always use immersion oil supplied by Olympus.

} If the objective in use can accommodate the oil-proof cap, be sure to

mount the cap.

1. Using a low-power objective, bring the specimen into focus.

2. Rotate the revolving nosepiece to engage the oil immersion objective.

3. Remove the specimen and move the stage insert cut-out 1 close to the
objective front lens. Apply a drop of the provided immersion oil to the
objective front lens. Place the specimen and rotate the fine adjustment
knob to bring the specimen into focus.

· Use as little oil as possible. After gently wiping off the oil on the
oil-proof cap, remove the cap. Then clean the tip of the objective
and areas around it as well as the cap.

· If the oil contains air bubbles, the image will be degraded. Make
sure the oil is free of air bubbles.

a) To check for air bubbles, remove the eyepieces, completely open the field

iris diaphragm and aperture iris diaphragm, and look at the objective exit
pupil (looking like a bright circle) in the observation tube. Any air bubbles
can be seen in this way.

b) To remove air bubbles, slightly rock the revolving nosepiece manually to

engage and disengage the oil immersion objective once or twice.

4. After use, remove immersion oil from the objective front lens by wiping
with gauze slightly moistened with absolute alcohol.

} The same procedure is applicable when using a water immersion

objective.

Caution on using the immersion oil:

If immersion oil comes into contact with your eye or skin,

immediately take the following action.
For eye: Rinse with clean water (for more than 15 minutes).
For skin: Wash with soap and water.
If the appearance of the eye or skin changes or pain continues,

immediately consult your doctor.

(Fig. 2)

10

IX81-ZDC2

11

Fig. 3

1

4 Placing the Specimen

Put the specimen in the glass-bottom dish (with cover glass thickness

of 0.16 to 0.18 mm) and place the dish on the center of the stage.

} If the dish is prone to slide on the stage, attach the stage clips (IX-SCL)

1 or use the provided weight.

(Fig. 3)

5 Setting the Focus Drift Compensation Function

1. Run the “Application Software for PC.”

2. Set up the AF (autofocusing) functions.
The following parameters should be set to start AF.

In one-shot mode

· Offset : Setup of the amount of compensation between the cover glass
top surface and target position.

· AF zone : AF search zone (AF center, width and range)

In continuous focus mode

Confirm that the cover glass top surface is detected in the one-shot

mode before switching to the continuous focus mode.

} For details, refer to the instruction manual and Help of the application.

CAUTION

When using the MetaMorph application software, install IX2-

BSW Ver. 1.7 (in the CD provided with this product) in your PC
in advance.

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4

SPECIFICATIONS

Item Specification

Applicable microscope frame IX81F-ZDC2, IX81S1F-ZDC2

Applicable observation tubes U-BI90-CT, U-BI90, U-TBI90, U-TR30H (in combination with the IX-ATU)

Applicable control box IX2-UCB (Control board and Application Software for PC (see page 1) are required.)

Controller PC

Focus detection method

Applicable objectives Refer to the list of applicable objectives (page 4).

Observation method Reflected light fluorescence observation is recommended. TIRF. DIC (compatible only with

Field number 22

AF focusing speed
(One-shot focus mode only)

AF repeatability · 20X and 40X objectives:

Dimensions & weight

Operating Environment · Indoor use.

Pupil-division reflection active autofocusing using a laser diode and 2-division detector.
Laser diode: 785 nm (Class 1: IEC60825-1, Class I : CDRH)
Laser pulse duration: 0.5 to 2.5 ms.
Frequency: 100 Hz. Momentary maximum output power; 127.5 [µW]

one-shot focus)
* The focus drift compensation function is not applicable to the phase contrast and polarized

light observations.

Approximately 1 to 5 sec. from the focusing range (Excluding the offset drive time)
(Note: The AF speed may slow down depending on the PC setup.)

±0.1 µm (with constant temperature),
±0.17 µm (with less than 5°C temperature change)

· 60X objective or 40X/60X/100X oil-/water-immersion objectives:
±0.07 µm (with constant temperature),
±0.1 µm (with less than 5°C temperature change)

IX81-ZDC2 system : 303 (W) x 313 (H) x 434 (D) mm., 19.2 kg (42.2 lb)

AF sensor unit : 76 (W) x 76 (H) x 201 (D) mm., 0.9 kg (2 lb)
* Image-forming lens section/excluding the connector

· Altitude: Max. 2000 meters.

· Ambient temperature: 18 to 40°C (64.4 to 104°F)

· Maximum relative humidity: 80% for temperatures up to 31°C (88°F), decreasing linearly

through 70% at 34°C (93°F), 60% at 37°C (99°F), to 50% relative humidity at 40°C (104°F).

· Supply voltage fluctuations: ±10%.

· Pollution degree: 2 (in accordance with IEC60664)

· Installation (overvoltage) category: II (in accordance with IEC60664)

IX81-ZDC2

5

Under certain conditions, performance of the microscope may be adversely affected by factors other than defects. If problems

occur, please review the following list and take remedial action as needed.

If you cannot solve the problem after checking the entire list, please contact Olympus for assistance.

a) Autofocusing is impossible.

TROUBLESHOOTING GUIDE

Problem Cause Remedy Page

The AF port switching lever is not set to IN.

Bubbles are present in the oil or water. Remove the bubbles.

An objective other than specified is in
use.

The objective is mounted improperly. Screw in the objective firmly.

The transmitted light bulb generates
infrared light.

A plastic bottom dish is in use. Use a glass bottom dish.

Electrical noise is interfering with op­eration.

The glass bottom dish has a large
scratch.

Set it to IN.

Use another objective or use manual
focusing.

Close the shutter or turn off the light
bulb during AF.

Ground the IX2-UCB. Be also sure to
ground the ancillary equipment.

Use another glass bottom dish or use
manual focusing.

8

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4

–

5

3

1

–

b) Autofocusing takes long time or

fails.

c) AF is applied to a position deviated

from the target position.

d) The specimen moves in the X-Y

directions during autofocusing.

A phase contrast objective is in use. Use the specified objective.

Immersion oil (or water) of oil- or water­Immersion objective is dried.

A water-immersion objective is used and
the interface between the water and
glass bottom dish is focused.

AF is set improperly. Set up AF properly.

Electrical noise is interfering with op­eration.

There is significant vibration in the in the
installed environment, or an anti-vibration
bench is not used.

The specimen is vibrating. Lock the specimen firmly. Also confirm

The offset value is input improperly. Enter the correct offset value. (For the

Immersion oil (or water) of oil- or water­Immersion objective is dried.

A water-immersion objective is used and
the interface between the water and
glass bottom dish is focused.

The specimen is not locked. Lock the specimen by placing a

Supply immersion oil (or water).

Set the AF zone so that its center is
located on the interface between the
culture fluid and glass bottom dish and
the AF zone width is ±10 µm.

(For the setting method, refer to the
instruction manual for the application
software.)

Ground the IX2-UCB. Be also sure to
ground the ancillary equipment.

Be sure to use an anti-vibration
bench.

that the stage center plate is free of
abnormality.

setting method, refer to the instruction
manual for the application software.)

Supply immersion oil (or water).

Set the AF zone so that its center is
located on the interface between the
culture fluid and glass bottom dish and
the AF zone width is ±80 µm.

weight on the glass bottom dish, for
example. Also confirm that the stage
center plate is free of abnormality.

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10

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Printed in Japan on May 19, 2010 M 003-01