Olympus IX81 User Manual
INSTRUCTIONS
IX81
MOTORIZED INVERTED
RESEARCH MICROSCOPE
This instruction manual is for the Olympus Motorized Inverted Research Microscope Model IX81.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the
microscope. Retain this instruction manual in an easily accessible place near the work desk for
future reference.
A X 7 3 2 0
CONTENTS
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to
assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 49 to 58) carefully. For the modules
provided with instruction manuals, also read the assembly procedures in their instruction manuals.
Note on operation in stand-alone mode
When the IX81 motorized revolving nosepiece is rotated in stand-alone mode (without a PC), automatic escape movement of an objective (prevention of interference between the objective and stage) is not performed.
Therefore, when using an objective with a short working distance, let the motorized revolving nosepiece
escape once before pressing the objective switching button.
IX81
IMPORTANT — Be sure to read this section for safe use of the equipment. —
1 MODULE NOMENCLATURE
2 CONTROLS
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE
3
4 USING THE CONTROLS
4-1 Microscope Frame .................................................................................................................................................................. 13-14
1 Voltage Indication 2 Light Path Selection
3 Magnification Change 4 Motorized Revolving Nosepiece
5 Frame Clamping Plate
4-2 Focusing Block...................................................................................................................................................................................... 15
1
Rotation Direction of the Focusing Knob
3 Focusing (UP/DOWN) buttons 4
4-3 Stage............................................................................................................................................................................................................ 16-17
1 Placing the Specimen 2 Moving the Specimen
3 Connecting the Grounding Wire 4
4-4 Observation Tube ..................................................................................................................................................................... 18-20
1 Adjusting the Interpupillary Distance 2 Adjusting the Diopter
3 Using the Eye Shades 4 Using Eyepiece Micrometer Disks
5 Selecting the Light Path of Observation Tube (U-TR30H Only)
6 Using the CT Turret (U-BI90CT Only) 7 Adjusting the Tilt (U-TBI90 Only)
4-5 Illumination Column (IX2-ILL100) ...................................................................................................................... 20-22
1 Tilting the Illumination Column 2 Mounting Filters
3 Using the Field Iris Diaphragm
4 Adjusting the Condenser Height Adjustment Knob Tension
5 Caution on Attachment of the Manipulator
2 Coarse/Fine Adjustment Switching Button
Objective Escape/Return (ESC) Button
Adjusting the X-Axis/Y-Axis Knob Rotation Tension
1-5
6
7-10
11- 12
13-27
4-6 Condenser......................................................................................................................................................................................... 23-25
1 Centering the Condenser 2 Using the Aperture Iris Diaphragm
3 Flipping Up the Condenser Holder
4-7 Objectives........................................................................................................................................................................................... 26-27
1 Oil-Proof Cap (UIS Series only) 2 Adjusting the Correction Collar
3 Using Immersion Objectives
IX81
5 OTHER OBSERVATION METHODS
5-1 Phase Contrast Observation .................................................................................................................................. 28-31
5-2 Differential Interference Contrast Observation............................................................................. 32-36
5-3 Simplified Polarized Light Observation ............................................................................................................. 37
5-4 Reflected Light Fluorescence Observation (Separate Manual).................................... 37
6 PHOTOMICROGRAPHY AND TV OBSERVATION
6-1 Photomicrography.................................................................................................................................................................. 38-40
6-2 TV Observation............................................................................................................................................................................. 41-42
7 TROUBLESHOOTING GUIDE
8 SPECIFICATIONS
9
ASSEMBLY
PROPER SELECTION OF THE POWER SUPPLY CORD ......................................................................
—
See this section for the replacement of the light bulb. —
28-37
38-42
43-45
46-48
49-58
59-60
10 LAMP HOUSING INSPECTION SHEET
This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.
N
OTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
61
IMPORTANT
The IX81 microscope can be used in stand-alone mode (operated from the U-HSTR2 hand switch) or can operated from a
PC when the IX2-BSW software (Ver. 01.03, compatible with Windows 2000/Me) is installed in the PC.
CAUTION
This microscope employs a UIS2/UIS (Universal Infinity System) optical design, and should be used only
with modules designed for the IX2 or BX2 series (which belong to the Olympus IX or BX series).
For the applicable modules, please consult Olympus or the latest catalogues. Less than optimum performance may result if inappropriate module combinations are used.
Configuration of Instruction Manuals
Since this microscope is expandable to a variety of systems, separate instruction manuals are prepared so
that the user has to read only the manuals according to the user’s own system.
Be sure to prepare the U-ZPCB (T2) Z-board and attach it to the IX81, and set the DIP switch (see page
50) before connecting the IX2-UCB control board to the IX81.
Manual Name Main Contents
IX81
IX2-UCB/U-HSTR2
IX2 Software for PC (CD-ROM)
*
Model name IX2-BSW (Ver. 01.03 or later)
Fluorescence system for IX2
Observation procedures including transmitted light brightfield,
phase contrast and DIC observations
Functions of the Control Box (incorporating the power supply)
and Hand Switch
Methods of PC control of functions
Reflected light fluorescence observation
U-FWT/FWR/FWO
IX2-MLWCD
IX2-DICD DIC condenser
Motorized filter wheels (The U-FWT cannot be used with this microscope.)
Mid long working distance condenser
Precautions When Unpacking the Microscope
Releasing the Transport Lock of the Motorized Revolving Nosepiece
#Never attempt to turn the system on without removing the clamping
rod. Otherwise, the focusing mechanism may be damaged.
1. Loosen the screw @ of the clamping rod using the Allen screwdriver
provided with the microscope frame.
2. Turn the nut ² of the clamping rod in the direction of the arrow until the
nut reaches the lowest height.
3. Likewise, turn the clamping rod ³ in the direction of the arrow until the
rod reaches the lowest height.
4. Insert the Allen screwdriver into the hole on the clamping rod mount 4
and turn the screwdriver in the opposite direction to the dial to remove
the clamping rod.
5. Attach the provided seal (10 mm dia., white) on the hole made after
removing the clamping rod to prevent penetration of dust through the
hole.
}Retain the clamping rod carefully because it will be used again the next
time the microscope is transported.
3
@
|
5
²
Fig. 1
1
SAFETY PRECAUTIONS
IX81
Releasing the Transport Lock of the Light Path Selector
#Never attempt to operate the light path selector without removing the
transport lock knob 5. Otherwise, the light path selector mechanism
may be damaged.
· Rotate the knob counterclockwise to remove it.
· To prevent penetration of dust through the hole made after removing the
transport lock knob, stop the hole by attaching the provided seal (10 mm
dia., black).
}Retain the knob carefully because it will be used again the next time the
microscope is transported.
Stage IX2-SFR/IX-MVR
· Before transporting the stage, fix the flexible handle with pieces of adhesive
tape so that it will not move.
1. After the equipment has been used in an observation of a specimen that
is accompanied with a potential of infection, clean the parts coming in
contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the
specimen. Be sure to remove the specimen before moving this
product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
2. If a foreign object is caught during motorized focusing operation, there
will be an error in the focusing block and the motorized focusing operation will be suspended.
Recovery procedure
· If there is no error in motorized operation, the caught object can be
removed by turning the focusing knob or focusing button.
·If there is an error in motorized operation, the focusing knob
or focusing button becomes inoperable. Disassemble the
relevant modules to remove the caught object. Replace the
relevant modules afterward.
· Turn off the power and then on again. The system will restart unless
there is a malfunction in the motor.
3. To activate temporary stop during focusing operation, turn the focusing
knob (or dial) on the microscope frame (in either direction) or press any of
the FOCUS control buttons ( , , F/C and ESC) (except during PC
communications).
When the main switch of the IX2-UCB control box is set to “ I " (ON),
the focusing operation starts automatically as part of initialization
(this operation consists of temporary raising and then returning to
the original position of the objective).
If any of the operations mentioned above is performed, an emergency
stop will also occur. In this case, set the main switch to “ " (OFF) and
then “ I " (ON) again.
4. When lowering the objective by pressing the focus button, be careful
not to have your hand caught between the bottom of the revolving
nosepiece and the microscope frame.
2
1
Fig. 2
2
3
5. The microscope is provided with a simplified waterproof mechanism.
Therefore, if culture liquid or water is spilt on the stage, revolving nosepiece
or microscope frame, damage to the equipment or an electrical shock
may result. Immediately wipe the liquid or water off if it is spilt on them.
6. When moving the microscope, remove the modules that may drop
including the specimen from the microscope in advance, then carefully carry the microscope frame by the base (front edge) 1 and the
grasping part on the illumination column 2 as shown in Fig. 2 (Weight:
approx. 25 kg).
When moving the microscope for a long distance, it is also recommended
to disconnect all cables from the equipment.
When transporting it, also engage the transport lock mechanisms and
package it sufficiently.
Also be careful against slipping of hands during carrying.
#Damage to the microscope will occur if you grasp it by other parts
including the stage, focusing knob, etc.
7. The microscope is not covered by warranty in terms of laser safety. The
user should assume liabilities for any consequence of user modification
including introduction of the use of laser beam.
8. The surfaces of the lamp housing will become extremely hot during
operation. When installing the microscope, make sure to allow ample free
space (10 cm or more) around and in particular above the lamp housing.
9. When installing the microscope, route the power cord away from the
lamp housing. Should the power cord come in contact with the hot lamp
housing, the power cord could melt and cause electric shock.
10. To avoid potential shock hazards and burns when replacing the light
bulb, set the main switch to “ ” (OFF) then disconnect the power cord
from the wall outlet in advance. Whenever you replace the bulb during
use or right after use, allow the lamp housing 3 and bulb to cool before
touching. (Fig. 3)
Fig. 3
Designated Bulb
11. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual. If the proper power cord is not used, product
safety performance cannot be warranted.
12. Always ensure that the grounding terminal of the microscope and that
of the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.
13. Never insert metallic objects into the air vents of the microscope frame
as this could result in electrical shock, personal injury and equipment
damage.
14. The standard service life of the lamp housing is 8 (eight) years of use or
20,000 hours of total power ON period, whichever is the shorter period.
For details, see Inspection Sheet on page 61.
12V100WHAL-L (PHILIPS 7724)
3
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
When raising or lowering the motorized revolving nosepiece, be careful not to have your finger
or hand caught by the mechanism.
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
Indicates that the main switch is ON.
IX81
Indicates that the main switch is OFF.
Warnings
Warning engravings are placed at parts where special precaution is required when handling and using the microscope.
Always heed the warnings.
Lamp housing
Warning engraving
position
Getting Ready
1
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations. (For
operating conditions, see Chapter 8, “SPECIFICATIONS” on page 48).
3. An intermediate attachment with a thickness of up to 60 mm can be mounted between the microscope frame and
binocular observation tube (U-BI90CT, U-BI90).
· If the U-BI90CT is used together with an intermediate attachment, the image may be cut off or obscured.
4. The oil-proof cap can only be mounted on a UIS Series 3 objective. Note that this does not change optical performance.
(For applicable objectives, see page 26.)
5. Restrictions in brightfield, phase contrast and DIC observations
· Deterioration in the optical performance will occur when the optional 2X magnification changer is installed in the
U-BI90CT.
(The deterioration can be improved by adding the U-EPA2 eye-point adjuster.)
· With the combination of U-TR30H + U-FWO, the full optical performance may not be able to be manifested with the
objectives listed in 1 below and it is not possible to use the objectives listed in 2 below. (This applies to objectives
other than Series 3.)
UIS2 Series
1UPlanSApo4X, 10X2, UPlanFLN4X, 10X2, 20X, CPlanFLN10X, LUCPlanFLN20X, UPlanSApo100XO
2PlanN40X, PlanApoN60XO
UIS Series
1UPlanApo4X, 10X, UPlanFl4X, 10X, 20X, CPlanFl10X, LCPlanFl20X, UPlanApo100XO
2Plan40X, UPlanApo40XOI3/340, PlanApo60XO3
(U-LH100L-3, U-LH100-3, IX-HLSH100)
Right side panel of microscope
(Warning against finger or hand
catching by mechanism)
(High Temperature
warning)
I3
4
6. Restrictions in TV observation
@The following combinations are not permitted in consideration of the optical performance.
· IX2-SPT + PE4X + U-PMTVC on the side port
· IX2-SPT + PE5X + 1X magnification changer + U-PMTV1X on the side port
· U-TV0.35XC + 2X magnification changer (optional)
· U-TV0.35XC + DP50 (optional 2X magnification changer on the side port)
²The following combination may deteriorate the optical performance a little.
· U-TV0.35XC + DP50 (UPlanSApo/UPlanApo4X/10X + 1X magnification changer on the side port)
7. Restrictions in fluorescence observation
· With combination of IX2-SHA + U-FWR (x 2) + U-LH100HGAPO, objectives UPlanSApo40X2, UPlanApo40X, UPlanFLN/
UPlanFl20X, UPlanFLN100XO2/100XOI2, UPlanFl100XO3, PlanApoN60XO and PlanApo60XO3 cannot be used due to
a problem in the optical performance. (This applies to objectives other than Series 3.)
8. Other
· The U-TRU or U-TVCAC cannot be mounted on the side port.
· When a large module is attached to the U-TR30H straight photo tube, it will be difficult to confirm the specimen.
· Only either the lower back port or left side port can be used.
Maintenance and Storage
2
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and
wipe gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the non-optical components of microscope. To clean them, use a lint-free,
soft cloth slightly moistened with a diluted neutral detergent.
3. Never attempt to disassemble any part of the microscope.
4. When not using the microscope, make sure to set the main switch to “ ” (OFF), confirm that the lamp housing is cool
enough and cover the microscope with the provided dust cover.
5
Caution
3
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
Intended use
4
This instrument has been designed to be used to observe magnified images of specimens in routine and research
applications.
Do not use this instrument for any purpose other than its intended use.
MODULE NOMENCLATURE
}The modules shown below are only the representative modules. As there are other modules which can be combined with
the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer.
For information on the modules marked with “*", refer to their instruction manuals.
Condenser
Transmitted Light Lamp Housing
· U-LH100L-3
· U-LH100-3 + U-RMT
· IX-HLSH100 + U-RMT
Transmitted Illumination
Column
IX2-ILL100
Reflected Fluorescence
Illuminator
· IX2-RFA*
· IX2-RFAL*
Reflected Light High-Intensity
Lamp Housing
· U-LH100HG*
· U-LH100HGAPO*
· U-LH75XEAPO*
High-Intensity Power
Supply Unit
· U-RFL-T*
· U-RX-T*
Eyepieces
· WHN10X
· WHN10X-H
Observation
Tube
· U-BI90CT
· U-BI90
· U-TBI90
· U-TR30H-2
(Used together
with IX-ATU)
· IX2-LWUCDA2
· IX2-LWUCD
· IX-ULWCD
· IX2-DICD*
· IX2-MLWCD*
· U-UCD8*
(Used together with IX-ADUCD)
Stage
· IX2-SFR
· IX-SVL2
· IX2-GS*
· IX2-SP
(Can be used
together with
IX-MVR)
IX81
*
Motorized Revolving
Nosepiece
(Fixed on the
microscope frame)
Microscope Frame
· IX81S1F-3
· IX81S8F-3
· IX81F-3
(Used together with
IX2-PRS8 or IX2-PRS1)
Fluorescence Mirror Unit
Cassette
· IX2-RFACA*
· IX2-RFAC*
Focus Adjustment Knob
Unit
U-FH
Hand Switch
U-HSTR2
Focus Adjustment Knob
Interface
U-IFFH
Control Box
IX2-UCB
(U-ZPCB(T2) installed
inside)
PC
(IX2-BSW Ver. 01.03
or later installed)
6
CONTROLS
}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 49 to 58).
· The illustration shows the system composed of modules enclosed in .
· For the reflected light fluorescence modules including the IX2-RFACA, IX2-RFA, U-LH100HG and IX2-UCB/U-HSTR2, refer to
their separate instruction manuals.
Condenser centering knobs
IX2-LWUCDA2
Aperture iris diaphragm
lever
Indicator plate
pockets
Light path selector
button (Page 13)
: Observation/
: Side port
Focusing buttons
(Page 15)
Filter pocket (Page 21)
Field iris diaphragm lever
U-LH100L-3
IX2-ILL100
Condenser height
adjustment knob
Column tilt
clamping screw
Stage center plate
IX81S1F-3
IX81S8F-3
IX81F-3
(Page 22)
(Page 20)
IX2-SFR
(Page 16)
Lamp voltage indicator
(Page 13)
Auxiliary button
Light intensity control
buttons (Page 13)
Transmitted light ONOFF button (Page 13)
7
Focusing knob (Page 15)
Detachable.
adjustment switching
Coarse/fine
button (Page 15)
Y-axis knob (Page 17)
X-axis knob (Page 17)
Magnification selector knob (Page
Pushed in: 1X
Pulled out: 1.6X
Objective escape/return button
(Page 15)
14 )
IX81
Left Side View of Microscope Frame
Allen screwdriver
storage position
(Rear panel)
Side port
Transport lock knob
#Be sure to remove this
before use.
Allen wrench and optical element
centering knob (x 2)
storage position (Rear panel)
U-BI90CT
Coarse/fine adjustment switching button (Page 15)Objective escape/return button (Page 15)
WHN10X/WHN10X-H
Diopter adjustment
ring (Page 18)
Interpupillary distance
adjustment scale
CT turret (Page 19)
Focus ring (Page 19)
Focusing knob (Page 15)
(Page 18)
Other Modules
Binocular Observation Tube
U-BI90
}Same as the U-BI90CT except that the U-BI90
does not have the CT turret and focus ring.
Tilting Binocular Observation Tube
U-TBI90
Trinocular Observation Tube H
U-TR30H-2
Straight photo tube
mount (Page 38)
Light path selector
button (Page 19)
Intermediate Attachment Tube
IX-ATU
8
Plain Stage
IX2-SP
Cross Stage
IX-SVL2
Y-axis knob
Mechanical Stage
IX-MVR
Manipulator mounting
screw holes
M6 screw (x 6)
IX-MVR mounting screw
holes (Page 52)
(On both sides)
Y-axis knob
X-axis knob
UCD Adapter
IX-ADUCD
X-axis knob
LWD Universal Condenser
IX2-LWUCD
}This is a manually controlled condenser.
Aperture iris diaphragm
lever (Page 23)
ULWCD Condenser
IX-ULWCD
}This condenser is manually operated.
Condenser height fine
adjustment knob
(Page 24)
Turret (Page 23)
Universal Condenser
U-UCD8
}This is a manually controlled condenser. Re-
fer to the separate instruction manual for details.
Aperture iris diaphragm
lever (Page 23)
9
Turret (Page 23)
Focus Adjustment Knob Unit U-FH
The button functions shown below are the initial setups for PC control (remote operation).
The button functions can also be assigned as required by the user. For the assignment, refer to the instructions for the IX2BSW IX Software.
After setting up the button functions, attach the provided stickers near the buttons. For the function abbreviations and
symbols on the stickers, see the following table.
IX81
Objective escape/shelter button
Coarse/fine adjustment
switching button
Focusing (DOWN) button
Auxiliary button
Abbreviations & Symbols Function Note
Fine/Coarse switching
Transmitted light ON/OFF
Set/cancel photo voltage
Focusing (UP) button
Focus adjustment knob
Connector
Connect to the U-IFFH.
Microscope (frame)/U-FH switching of
focus adjustment knob
Z-focusing motor ON/OFF OFF: Electrical noise reduction
Escape/return objective
Shutter IN/OUT
Condenser top lens IN/OUT Not used with the IX81.
Up/down operation for brightness adjustment, objective, etc.
Left/right operation of mirror unit, filter
wheel, etc.
The function name can be written
in the blank area using an oil-ink
pen.
10
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic obser-
vation method of this microscope. The operating procedures for phase contrast observation and DIC observation will be
described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 28.
For the fluorescence observation, refer to the separate instruction manual entitled “Reflected Fluorescence System”.
Set the main switch to “ I ” (ON).
Disengage the DIC slider and
analyzer from the light path.
Disengage the filter other than the
frost filter from the light path.
Engage a 10X objective in the light path.
Select the brightfield observation by pressing
the BF button* and select the (observation)
light path.
Place the specimen on the stage.
Bring the specimen in focus. 8Focusing knob (dial) (P. 15)
Adjust the brightness. 9Light intensity control buttons (P. 13)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
(Controls Used)
1Main switch: “ I ” (ON) (P. 13)
2Transmitted light ON-OFF button: ON (P. 13)
3Filter pocket (P. 21)
7Objective switching button (P. 14)
4BF observation button*
5Light path selector button (P. 13)
6X-axis and Y-axis knobs (P. 17)
Focus adjustment knob or
focusing button
aBinocular tube (P. 18)
bDiopter adjustment ring (P. 18)
cCondenser height adjustment knob (P. 23)
dCondenser centering knobs (P. 23)
eField iris diaphragm lever (P. 22)
(Page)
11
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the aperture iris and field iris diaphragms.
Engage the required filters. 3Filter pocket (P. 21)
Adjust the brightness.
Start observation.
7Objective switching button (P. 14)
8Focusing knob (dial) (P. 15)
Focus adjustment knob
fAperture iris diaphragm lever (P. 25)
eField iris diaphragm lever (P. 22)
9Light intensity control buttons (P. 13)
* Usable when the DIA OBS setting is made in the IX2-BSW (Ver. 01.03 or later) software.
The button functions shown below are the initial setups at the moment the PC is started up.
Controls with numbers inside are not available when the U-FH focus adjustment knob unit is in use.
3
e
f
b
d
c
IX81
a
5
9
2
8
8
8
6
7
4
1
} Make a photocopy of the observation procedure pages and post it near your microscope.
12
USING THE CONTROLS
4-1 Microscope Frame
6
4
5
Fig. 4
1
2
3
Voltage Indication
1
}Set the main switch of the IX2-UCB control box to “ I ” (ON), then press the
transmitted light ON-OFF button 1 to ON.
1. Press the light intensity control button 2 to increase the voltage and
make illumination brighter.
Pressing the button 3 makes the illumination darker.
2. The numerals to the right of the lamp voltage indicator LEDs 4 indicate
the reference values of the voltages.
#The LEDs may turn off (temporarily) when the motor is driven, but the
illumination intensity does not vary even if this occurs.
3. The marking 5 is the reference position indicating 9 V, which is the
suitable intensity for color photography (using LBD filter).
Light Path Selection
2
}The light path selector button 6 allows for light path switching between
the observation and side port paths.
The selected light path can be confirmed with the LED on the side of the
button.
: Observation 100% light path.
: Side port 100% light path (with the IX81S1F-2 microscope frame)
Side port light path 80%/Observation 20% light path
(with the IX81S8F-2)
}The light path to the lower back port can also be set by the manufacturer’s
part replacement operation. (IX2-LBPC)
(Fig. 4)
(Fig. 4)
13
IX81
Fig. 5
1
Magnification Change
3
When the magnification selector knob 1 is pulled out, the magnification
will be 1.6X. When the knob is pushed in, the magnification will be 1X.
}The 1X 1.6X magnification changer lens can be replaced with a 1X 2X
magnification changer lens (IX2-CA2) by the manufacturer operation.
Motorized Revolving Nosepiece
4
# When the IX81 motorized revolving nosepiece is rotated in stand-alone
mode, automatic escape movement of an objective is not performed.
Therefore, let the motorized revolving nosepiece escape once before
turning the nosepiece.
The motorized revolving nosepiece can be rotated to switch the objective by pressing an appropriate button on the U-HSTR2 hand switch or a
button assigned by the PC.
}When the IX2-BSW software (Ver. 01.03 or later) is started up, the revolving
nosepiece escapes to avoid interference with the stage as the objective
is switched, and returns to the original position after objective changing.
#It is basically inhibited to attempt to rotate a motorized revolving
nosepiece manually.
However, it can be moved to the front and rear by hand only when an
oil immersion objective is used and bubbles are produced inside
the oil.
(Fig. 5)
Frame Clamping Plate
5
}This is the module for clamping the microscope frame onto an anti-
vibration platform. The applicable anti-vibration platforms are the following four models.
· 25 mm pitch and 50 mm pitch anti-vibration platforms.
· 1-inch pitch and 2-inch pitch anti-vibration platforms.
For the assembly procedure, see item
9 on page 58.
14
4-2 Focusing Block
}The same effect as the focusing knob on the microscope frame can also be obtained using the U-FH focus adjustment
knob unit. However, when the microscope is used stand-alone while the cable to the U-FH is connected, the control used
for focus adjustment is switched automatically to the focus adjustment knob on the U-FH.
2
1
Fig. 6
2
1
3
Rotation Direction of the Focusing Knob
1
· Rotating the focus adjustment knob 1 toward the front (in the direction of
the arrow) raises the objective and toward the rear (opposite direction)
lowers the objective.
Coarse/Fine Adjustment Switching Button
2
}This button switches the motion of the objective controlled by the focusing
knob 1 between coarse and fine adjustment.
· The fine adjustment stroke is 0.5 mm per turn with the initial setting, but
this can be changed using the IX2-BSW software,
· To select the desired adjustment stroke, press the coarse/fine adjustment
switching button 2.
Coarse adjustment: 1 mm per turn
Fine adjustment: 0.5 mm per turn
Focusing (UP/DOWN) buttons
3
#The focusing speed available with these buttons is coarse (3 mm/
sec.) only. The focusing speed can be changed with a PC.
· The objective ascends as the focusing button @ is kept pressed and
stops when the finger is released from the button.
· The objective descends when the focusing button 2 is pressed.
(Fig. 6)
(Fig. 6)
(Fig. 7)
15
Fig. 7
Objective Escape/Return (ESC) Button
4
· When replacing the specimen, press the ESC button 3. The objective will
lower to the lowest position. Pressing the button again returns the objective
to the original height.
· When the IX2-BSW software's “STOP" button is pressed after the objective has been escaped, the return operation will be cancelled.
(Fig. 7)
4-3 Stage
IX81
1
Fig. 8
Fig. 9
1
Placing the Specimen
1
With the IX2-SFR or IX-SVL2 Stage (Fig. 8)
Place the specimen on the center of the stage.
}In the case of a slide glass specimen, plate the specimen with the cover
glass facing down.
}If the specimen is prone to slide on the stage, attach the stage clips (IX-
SCL) 1 and clamp the specimen down with the clips.
With the IX-MVR Mechanical Stage + IX2-SP Stage (Figs. 9 & 10)
}96-well or 24-well microtiter plates, etc. are held in place by the speci-
men holder.
Microtiter plates with dimensions of max. 136 mm x 92 mm can be accommodated in this way.
1. Open the spring-loaded finger of the specimen holder 1 and slide the
microtiter plate into the holder frame. Gently release the curved finger to
clamp. (Fig. 9)
(Figs. 8 to 10)
4
3
2
Fig. 10
5
}To secure other vessels than microtiter plates, various optional holders
are available. A Terasaki plate holder 2 is available for holding Terasaki
plates (72-well, 60-well). When using this, it is necessary to replace the
stage scales with those provided with the plate holder. Petri dish holder
3 is available for 35 mm, 54 mm and 65 mm diameter petri dishes, a
slide glass holder 4 is available for holding slide glass, and the IX2BCTP* 5 is available for a blood cell test plate holder. (Fig. 10)
* A blood cell test plate or other calculating chamber for bacteria and
eosinophil with mounting section dimensions corresponding to H 77 x
+0.3
0
V 35 x D 2 mm can be used. A 60 mm diameter petri dish can also be
used.
+0.3
0
16
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