IX2-ARCEVA
IX2-ARCEVA
ARC ILLUMINATION TIRF SET
This instruction manual is for the Olympus Arc Illumination TIRF Set. To ensure the safety, obtain
optimum performance and to familiarize yourself fully with the use of this system, we recommend that
you study this manual thoroughly before operating the system.
As this system employs an arc light source (mercury burner or xenon burner), take special care for safety
and please also read the instruction manual for the light source.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
INSTRUCTIONS
A X 7 3 8 7
IX2-ARCEVA
CONTENTS
Correct assembly and adjustments are critical for the system to manifest its full performance. If you are going to assemble
the system by yourself, please read chapter 3, “ASSEMBLY” (pages. 8 to 11). For the assemblies of the modules for which
instruction manuals are available, refer to their instruction manuals.
IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-4
1 NOMENCLATURE 5
2 CONTROLS 6-7
3 ASSEMBLY 8-11
4 OPERATION 12-14
4-1 Preparation
1 Centering the Burner
2 Adjusting the Slit
4-2 Total Reflection Fluorescence Observation
..............................................................................................................................................................................................................
....................................................................................................................
12-13
14
5 TROUBLESHOOTING GUIDE 15
IMPORTANT
This system has been designed to enable total reflection fluorescence observation using an arc light source
(mercury burner or xenon burner).
Total reflection illumination is implemented by limiting the illumination projection area to the area corresponding
to the objective pupil NA of 1.39 (because the reflectance of cytoplasm is 1.38).
}This system offers the following advantages compared to the laser-based total reflection illumination system.
· Higher safety, because the laser light is not used.
· Easy handling without the care of interference stripe, because the light source is not a coherent source.
· Easy switching between multiple wavelengths.
Nevertheless, this system is not applicable to molecular fluorescence observation due to low intensity of the
illumination. Its applications are limited to high-contrast observations of tissues near cell membranes and
physiochemical phenomena.
SAFETY PRECAUTIONS
1. Do not ignite the mercury burner or xenon burner without mounting the lamp housing on the microscope.
The UV light emitted from the burner is hazardous for your eyes. Do not look directly at the light output from the lamp
housing.
For details precautions, also refer to the instruction manual for the light source.
2. When centering the burner or replacing the specimen, be sure to look them through the UV cut plate to protect your eyes.
1
IX2-ARCEVA
Getting Ready
1
1. This manual pertains only to the handling of the total reflection fluorescence illumination system. Please also read the
instruction manuals for the IX2 series (IX81/IX71/IX51) microscope and associated options to understand the comprehensive operating instructions of the microscope system.
2. The total reflection fluorescence illumination system is composed of precision instruments. Handle it with care and avoid
subjecting it to sudden or severe impact.
3. Do not use the system where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.
4. Reserve spaces of 10 cm or more around the lamp housing and power supply to facilitate their ventilation.
5. The fluorescent light illuminator that can be used in combination with this system is only the IX2-RFAL L-shaped reflected
light illuminator.
6. The objectives that can be used with total reflection illumination are only the PlanApoN60XO
7. The illumination field of total reflection fluorescence observation is limited within the area of field number 11.
8. Since the total reflection light is not point-source light, the excitation light incidence angle on the specimen is not
constant and the effusion depth cannot be specified.
9. If an excitation filter is included in the mirror unit during total reflection fluorescence illumination, the repeated reflections
cause the leak of direct light to increase and degrade the contrast considerably. To prevent this, remove the excitation filter
from the mirror unit and mount in the excitation filter slider.
10
. The size of the excitation filter inserted in the filter slot of the excitation slider should be 25 mm in diameter and 6 mm or
less in thickness. Only one ND filter with a diameter of 32 mm and thickness of 1 mm or less can be inserted.
11
. When ordinary fluorescence observation is performed by inserting a 25 mm excitation filter in the excitation filter slider,
the illumination field is limited within the area of field number 20.
. To prevent light leak, be sure to attach the provided 32 mm light shields in the unused positions of the excitation filter
12
slider.
and PlanApo60XO
TIRFM
TIRFM
.
2
13
. The aperture iris diaphragm for use in ordinary fluorescence observation is not provided. If it is required to use the aperture
iris diaphragm, replace the slit iris diaphragm unit of this system with the aperture iris diaphragm unit of the IX2-RFAL.
14
. When the IX2-RFAC, IX2-RFACA and a standard mirror unit are used in combination, it is required to readjust the centering
of the slit iris diaphragm unit because, otherwise, the contrast of the total reflection fluorescent light would fluctuate due
to variation in the illumination light projection positions between turret positions.
15
. The IX2-SFR right-hand knob stage is not recommended because the stage knobs will degrade the operability of the slit
iris diaphragm unit considerably. Instead, it is recommended to use the IX-SVL-2.
16
. When the IX2-RSPC right-side port adapter is used, some positions of the excitation filter slider would be unusable due
to interference with the camera. The following table shows the relationship between the camera size and unusable filter
positions.
3
Height from the center of
C-mount
Less than 27 mm
27 mm to 45 mm exclusive
45 mm or more
}The camera interference can be reduced by using the U-FWR filter wheel or a commercially available motorized filter
wheel.
Depth from the center of
C-mount
Less than 34 mm None
34 mm to 70 mm exclusive Deepest (vacant) position is unusable.
70 mm or more The two deepest positions are unusable.
Less than 20 mm None
20 mm to 56 mm exclusive Deepest (vacant) position is unusable.
56 mm or more The two deepest positions are unusable.
Interference condition
None
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