OLYMPUS CELLM R 3.2 User Manual [ru]

®
INSTRUCTIONS
&
IMAGING HARDWARE FOR
LIFE SCIENCE MICROSCOPY
This instruction manual describes the hardware components of the Olympus and Imaging Stations. To ensure safety, obtain optimum performance and familiarize yourself fully with the use of these products, we recommend that you study this manual thoroughly before operation. Together with this manual, please also read the and software manual and the instruction manual of the microscope in order to understand overall operation methods. Retain this manual in an easily accessible place near a system for future reference.
OLYMPUS SOFT IMAGING SOLUTIONS GMBH
Rupert-Mayer-Strasse 44 D-81379 München Tel: +49 89 - 89 55 805 660 Fax +49 89 - 89 55 805 6606 Email: info@olympus-sis.com
www.olympus-sis.com
Imaging Excellence
We at Olympus Soft Imaging Solutions GmbH have tried to make the information in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any matter whatsoever relating to this manual, including without limitation the merchantability or fitness for any particular purpose. Olympus Soft Imaging Solutions GmbH will from time to time revise the software described in this manual and reserves the right to make such changes without obliga­tion to notify the purchaser. In no event shall Olympus Soft Imaging Solutions GmbH be liable for any indirect, special, incidental, or consequential damages arising out of purchase or use of this manual or the information contained therein.
No part of this document may be reproduced or transmitted in any form or by any means, electronic or me­chanical, for any purpose, without the prior permission of Olympus Soft Imaging Solutions GmbH.
2003 – 2009 by Olympus Soft Imaging Solutions GmbH. All rights reserved.
Manual version: March 2009
ΧΧΧΧ Declaration of Conformity
Robert-Koch-Strasse 9 D-82152 Planegg
declares under its own responsibility that the following products
Imaging Station for Life Science Applications
and
Imaging Station for Life Science Applications
to which this declaration refers are in conformity with the
Low Voltage Directive 73/23/EEC and the EMC Directives 89/336/EEC and 93/68/EEC
and the following standards:
EN 61010-1: 2001 (Low Voltage) EN 61326:1997 + A1: 1998 + A2: 2001 (EMC).
Michael Czempiel and Dr. Matthias Seel, Managing Directors
June 11, 2003 and
October 08, 2005
& Hardware Manual Contents
Contents
1 Introduction and Overview .................................................................................1
1.1 Abstract .............................................................................................................. 2
1.2 Technical Support ............................................................................................... 2
1.3 Symbols .............................................................................................................. 3
1.4 Checklist: cell^M / cell^R Components................................................................. 4
1.4.1 Standard Components: ....................................................................................... 4
1.4.2 Additional Components....................................................................................... 5
2 Illumination System MT10 / MT20.......................................................................7
2.1 Safety Precautions and Handling Instructions...................................................... 8
2.1.1 MT20 / MT10 Safety Precautions ......................................................................... 8
2.1.2 Special Safety Precautions Regarding the Arc Burners......................................... 9
2.1.3 Handling Instructions and Recommendations.................................................... 10
2.1.4 Safety Labels......................................................................................................11
2.2 Illumination System MT10 / MT20 Overview...................................................... 12
2.2.1 The Front Panel................................................................................................. 12
2.2.2 Top: the Burner Flap...........................................................................................13
2.2.3 Right Side: the Filter Wheel Flap.........................................................................13
2.2.4 The Back Panel.................................................................................................. 14
2.2.5 Operation.......................................................................................................... 14
2.2.6 Specifications and Technical Data of the MT10 / MT20 .......................................15
2.2.7 Fuse Exchange .................................................................................................. 16
2.2.8 Specifications and Technical Data of the Real-Time Controller /
System Coordinator Connectors........................................................................ 16
3 Epi-Fluorescence Illuminators ........................................................................... 17
3.1 Features ............................................................................................................ 18
3.1.1 Light Guide Port ................................................................................................ 18
3.1.2 Adjustment Screws for Centering the Field of Illumination ................................ 18
3.1.3 Filter Slider for Dispersion Filter and Exciter Balancers ...................................... 19
3.1.4 Light Sensor ...................................................................................................... 19
3.2 IX2 Illuminators with Field Stop Slider ............................................................... 19
3.3 Flange for the IX2 Microscopes .......................................................................... 20
3.4 Fluorescence Illuminator Unit for the BX2 Microscopes: BX_MTA, BX_MTAA.... 20
3.5 Fluorescence Illuminator Unit for the MVX Microscope...................................... 21
4 System Assembly and Adjustment .................................................................... 23
4.1 Overview: Connectivity of System Components ................................................ 24
4.2 Connecting the Modules of the cell^M / cell^R Imaging Station ......................... 25
4.2.1 Connecting the MT10 / MT20............................................................................. 25
4.2.2 Mounting and Connecting the Epi-Fluorescence Illuminator .............................. 26
4.2.3 Connecting the Camera......................................................................................27
4.2.4 Connecting the Imaging PC to the Modules ....................................................... 28
Contents
4.2.5 Configuring the Imaging PC Network Connections ............................................29
4.3 Preparing the Illumination System MT10 / MT20................................................ 30
4.3.1 Burner Installation and Exchange....................................................................... 30
4.3.2 Burner Alignment .............................................................................................. 31
4.3.3 Filter Exchange .................................................................................................. 33
4.4 Centering the Illumination ................................................................................. 35
4.5 Optimizing the Illuminator Tube Position........................................................... 36
5 Optional Hardware Modules .............................................................................39
5.1 Mounting and Connecting the Piezo-Electric Objective Drive (PIFOC)................ 41
5.2 Connecting the Piezo-Electric Nosepiece Drive (Nosepiece PIFOC for
IX2 Microscopes)................................................................................................ 43
5.3 Connecting the Corvus Controller of a Motorized Märzhäuser Stage ..................44
5.4 Installing Illuminators with Pinhole Field Stop Sliders......................................... 45
5.4.1 Centering the pinhole ........................................................................................46
5.5 Installing the OptiGrid ....................................................................................... 47
5.5.1 The OptiGrid Illuminator.................................................................................... 47
5.5.2 Connecting the OptiGrid.................................................................................... 47
5.5.3 Configuring the OptiGrid ...................................................................................48
5.5.4 Optical Alignment of OptiGrid and Camera........................................................49
5.5.5 Calibrating the OptiGrid.....................................................................................50
5.5.6 Calibrating the OptiGrid Z-Position for Different Image Types ........................... 52
5.5.7 Stripe Filter for Snapshots ................................................................................. 54
& Hardware Manual Chapter 1 – Introduction and Overview 1
1 Introduction and Overview
Thank you very much for purchasing Olympus’ state of art Imaging Station cell^M or cell^R and for your confi­dence in our products and service. It is Olympus main objective to provide you with solutions able to meet your experimental demands and thus pave the way to your scientific success.
1.1 Abstract .............................................................................................................. 2
1.2 Technical Support ............................................................................................... 2
1.3 Symbols .............................................................................................................. 3
1.4 Checklist: cell^M / cell^R Components................................................................. 4
1.4.1 Standard Components: ....................................................................................... 4
1.4.2 Additional Components....................................................................................... 5
2 Chapter 1 – Introduction and Overview
1.1 Abstract
The Imaging Station cell^M / cell^R consists of the following hardware components:
Illumination System MT10 / MT20
Illumination Coupling to the microscope (epi-fluorescence illuminator with light fiber)
Real-time Controller (cell^R) / System Coordinator (cell^M)
controller board inside PC
CCD camera (several models optional)
Optional peripherals such as piezo-electric z-drive (PIFOC)
You will find a detailed description of these components, handling and alignment instructions as well as advice how to maintain the performance of your system on the following pages. Keep in mind that all the components are designed to work together as an integrated system. Taking away or replacing single components without prior consultation of Olympus BioSystems is strongly discouraged and will most probably impair the perform­ance of the system dramatically. Any damages to the system due to such mishandling will not be covered by the guarantee.
All necessary tools for handling and alignment are shipped with the system.
The Imaging Station cell^M / cell^R is compatible with the following Olympus microscopes: IX50, IX70, IX51, IX71, IX81, BX50, BX50WI, BX51, BX51WI, BX61, BX61WI. Other microscopes may be supported on request.
An additional installation of a standard Olympus epi-fluorescence Xe, Xe/Hg or Hg lamp house (e.g., U-LH100HGAPO, U-LH75XEAPO) is not possible because the OBS epi-fluorescence illuminator occupies the microscope’s fluorescence excitation port.
This instructions manual is for the hardware components of the Olympus BioSystems Imaging Station cell^M / cell^R. To ensure safety and optimum performance as well as to familiarize yourself with the use of the system we recommend you to study this manual thoroughly before operating the system. Keep this manual in an easily accessible place near the Imaging Station for future reference.
The cell^M / cell^R system meets the CE, UL and FCC requirements.
IEC/EN 61010-1(2nd ed.), UL61010-1,
EN61326 Class A, FCC Part 15 Class A (Illumination System MT10 / MT20: class A and B).
1.2 Technical Support
If you find any information missing in this manual or you need additional support, please contact your local Olympus representative.
& Hardware Manual Chapter 1 – Introduction and Overview 3
1.3 Symbols
The following symbols are placed on the Illumination System MT20 / MT10. Study the meaning of the symbols and always use the equipment in the safest possible manner.
General symbols
Symbol Explanation
Carefully read the instructions on the label and the manual before use. Improper use could result in personal injury to the user and/or damage the equipment.
The surface becomes hot and should not be touched with bare hands
Parts may be under high voltage and should not be touched.
The main switch is turned OFF.
The main switch is turned ON.
This symbol indicates separate collection of waste electrical and electronic equipment in the EU
countries.
Do not throw the equipment into the domestic refuse
Please use the return and collection systems available in your country for the disposal of this
product
4 Chapter 1 – Introduction and Overview
1.4 Checklist: cell^M / cell^R Components
Before assembly, please check whether you have the complete set of cell^M / cell^R system components as listed below.
1.4.1 Standard Components:
a Microscope, upright or in various configurations.
b Illumination System MT10 / MT20 consisting of:
MT10 / MT20 housing with integrated lamp house, filter wheel, shutter and attenuator
short arc burner
power cord
Illumination System connector cord (blue RJ45 cable)
light sensor connector cord (see photo in Chapter 7, System Assembly and Adjustment)
optical fiber (quartz light guide; see photo in Chapter 7, System Assembly and Adjustment)
set of hex-wrenches
cotton gloves
c Fluorescence filter set consisting of:
Dichroic mirror and emission filter mounted in Olympus filter cube
Excitation filter(s) to be mounted in the MT10 / MT20 filter wheel
d MT10 / MT20 epi-fluorescence illuminator to couple the illumination system to the microscope via the optical
fiber
e CCD camera (or EMCCD camera) of choice, typically with
FireWireTM (IEEE 1394) cable
trigger cable
f cell^R / cell^M imaging computer including
cell^R Real-time Controller board / cell^M System Coordinator board
cell^R / cell^M network card
FireWireTM (IEEE 1394) card
front panel with three digital I/O ports (optional for cell^M)
cell^R / cell^M imaging software installed
computer monitor with cable
2 power cords
software protection key (dongle)
g cell^R / cell^M imaging software on CD-ROM
h Manuals
& Hardware Manual Chapter 1 – Introduction and Overview 5
1.4.2 Additional Components
The following components are optional and may or may not be part of your cell^R / cell^M imaging workstation.
a Motorized microscope modules in various configurations; all include
universal microscope control box (UCB)
microscope manual control (optional)
power cord
connection cords
b Fast piezo-electric objective mover (PIFOC, Physik Instrumente GmbH, see Chapter 5.1, Mounting and Con-
necting the Piezo-Electric Objective Drive PIFOC) consisting of
Objective mover
PIFOC controller
power cord
PIFOC connector cable
spacers to lift the stage (for Olympus IX microscopes)
objective distance rings
extension cords
c Fast piezo-electric nosepiece mover (nosepiece PIFOC, Physik Instrumente GmbH, see Chapter 5.2, Mount-
ing and Connecting the Piezo-Electric Objective Drive PIFOC) consisting of
nosepiece mover
nosepiece PIFOC controller
power cord
PIFOC connector cable
extension cords
d motorized stage by different manufacturers
e climate chamber
f Optigrid for resolution enhancement via structural fluorescence illumination
6 Chapter 1 – Introduction and Overview
& Hardware Manual Chapter 2 – Illumination System MT20 / MT10 7
2 Illuminat ion System MT10 / MT20
This chapter refers only to the fluorescence Illumination System MT10 / MT20. Before using this unit together with the other system components, the microscope and associated devices, make sure that you have carefully read and understood their respective manuals, and understand how the entire system should be operated.
2.1 Safety Precautions and Handling Instructions...................................................... 8
2.1.1 MT20 / MT10 Safety Precautions ......................................................................... 8
2.1.2 Special Safety Precautions Regarding the Arc Burners......................................... 9
2.1.3 Handling Instructions and Recommendations.................................................... 10
2.1.4 Safety Labels......................................................................................................11
2.2 Illumination System MT10 / MT20 Overview...................................................... 12
2.2.1 The Front Panel................................................................................................. 12
2.2.2 Top: the Burner Flap...........................................................................................13
2.2.3 Right Side: the Filter Wheel Flap.........................................................................13
2.2.4 The Back Panel.................................................................................................. 14
2.2.5 Operation.......................................................................................................... 14
2.2.6 Specifications and Technical Data of the MT10 / MT20 .......................................15
2.2.7 Fuse Exchange .................................................................................................. 16
2.2.8 Specifications and Technical Data of the Real-Time Controller /
System Coordinator Connectors........................................................................ 16
8 Chapter 2 – Illumination System MT20 / MT10
2.1 Safety Precautions and Handling Instructions
For Laboratory Use Only.
Danger: Ultraviolet radiation is emitted from this product. Avoid exposure. ALWAYS WARE
PROTECTIVE CLOTHING. EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER. ALWAYS WARE PROTECTIVE EYEWEAR; FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURE TO THE EYE. Never look directly into the lamp. Exposure can cause eye and skin allergy and allergic reactions. Medications or cosmetics may increase your sensi­tivity to ultraviolet radiation. Consult a physician before operating this product if you are using medications or have a history of skin problems or believe yourself particularly sensitive to sunlight.
Always have the microscope's UV cut plate mounted as protection against UV radiation.
Color fading of specimens: This system features high excitation light intensity to ensure bright ob­servation of dim fluorescent specimens. Consequently, after longer periods of observations using high-power objectives, the colors of specimens will fade quicker than usual, causing the intensity and contrast of fluorescent images to deteriorate. In such cases it is advisable to reduce excitation light intensity to slow down color fading. To reduce the light intensity use the built-in attenuator as explained in this manual.
Engage the shutter of MT20 / MT10 as explained in this manual (or use the shutter of the micro-
scope) even if you interrupt observation only for a short time.
2.1.1 MT20 / MT10 Safety Precautions
a Always use a power cord provided or approved by Olympus BioSystems.
b Provide unimpaired access to the main power switch at the rear panel.
c No electrical cord should be connected or disconnected while the power switch is set on (“I”). Make sure that
the unit's main power switch is set off (“O”) before plugging or unplugging the power cord to the power out­let.
d Always make sure that the grounding terminal of the Illumination System MT10 / MT20 and the wall outlet
are connected properly. If the System is not grounded, Olympus BioSystems cannot warrant the electrical safety and performance of the system.
e When installing the Illumination System MT10 / MT20 leave at least 100 mm free air space at the rear panel
and at least 50 mm at the sides and on top to allow air circulation. The Illumination System MT10 / MT20 has an internal fan to cool the arc burner. The air outlet is located at the rear of the housing while the inlet grid is at the bottom.
& Hardware Manual Chapter 2 – Illumination System MT20 / MT10 9
f The Illumination System MT10 / MT20 must be placed on an even, stable and flat surface and standing on its
supports. Make sure that no cloth or paper can clog the air inlet slits at the bottom of the housing.
g The arc burner has to be exclusively one of the two types specified by Olympus BioSystems. No other arc
burner may be used with the Illumination System MT10 / MT20.
h Do not open the burner flap of the Illumination System MT10 / MT20 while the arc burner is turned on. First
turn off the arc burner by software and wait for at least 30 minutes while the cooling fan is operating. Set the main power switch on “O” (OFF) before opening the burner flap. The arc burner socket and all parts in the vi­cinity of the burner will be extremely hot during operation and will cause thermal injury if touched.
i Never attempt to disassemble the Illumination System MT10 / MT20 or to open its housing. The internal
power supply units and electrical circuits contain high voltage components and can cause severe electrical shock and thus are not serviceable by the user. Warranty will be lost if the housing is opened.
j Do not operate the Illumination System MT10 / MT20 under environmental conditions other than specified in
this manual (see Chapter 2.2.6, Specifications and Technical Data).
k Do not look directly into the light emitted by the arc burner with unprotected eyes under any circumstances.
The emitted light is very intense and has a very high UV-content that may cause irreversible damage to your eyes.
l Avoid direct exposure of unprotected skin to the light emitted by the arc burner. It can cause severe burns.
m Direct exposure to the arc burner light may occur at the following positions:
Illumination System’s housing at the fiber port if the fiber is removed
Exit of the optical fiber
Exit of the illumination coupling (epi-fluorescence illuminator)
Beam path of the microscope (which upon proper usage is not accessible by the user)
Exit of the objective (or at the nose piece, if no objective is mounted)
n If the equipment is used not as specified by this manual, the safety and performance of the equipment may
be impaired. In addition, the equipment may also be damaged and warranty may be lost. Always use the equipment as outlined in this instruction manual.
2.1.2 Special Safety Precautions Regarding the Arc Burners
a Handling the arc burner bulb is potentially dangerous. Wear a protective mask, leather gloves and a thick
long-sleeved shirt or coat during handling and disposal (see below).
b The arc burner bulb is under high internal pressure. Do not hit the arc burner bulb against anything nor
scratch it or apply excessive stress. Avoid vibrations. Otherwise the arc burner bulb could explode and cause injuries.
c For transport and storage always place the arc burner in the provided protective case.
d Take care to avoid leaving fingerprints or dirt on any part of the high-pressure arc burners. If contaminated,
clean by wiping gently with gauze. To remove fingerprints or oil stains, wipe with gauze slightly moistened with lens cleansing fluid. Since these fluids are highly inflammable, be careful to keep them away from open fire, heat or hot surfaces and potential sources of electrical sparks, such as main switches.
10 Chapter 2 – Illumination System MT20 / MT10
e The arc burners have an average lifetime as specified in this manual (see Chapter 2.2.6, Specifications and
Technical Data). When the arc burner hour counter (see Chapter 4.3.2, Burner Alignment) gives a reading
higher than the specified lifetime, replace the burner with a new one following the instructions for replace­ment (see Chapter 4.3.1, Burner Installation and Exchange) and disposal of used burners (see below). Arc burners that are used longer than the rated service lifetime will have a reduced intensity and stability; they are likely to fail and might explode because of the deteriorated glass condition.
f Arc burners not in service must always be kept in the provided protective case.
g Hg/Xe burner bulbs contain poisonous mercury. They have to be treated with special care. If they explode
during service mercury gas might be dispersed in the environment.
h Hg/Xe burners must be treated as hazardous waste. Always follow national and local laws and guidelines
when disposing of Hg/Xe burners. It is recommended to commission a professional waste disposal company with the disposal. Hand over the burner in its original packaging.
i Xe burners: It is recommended to commission a professional waste disposal company with the disposal.
Hand over the burner in its original packaging. Disposal without breaking the glass part may result in burner bulb explosion. Follow the instructions below to prevent harmful repercussions.
Ware protective clothing.
Wrap the used lamp in a thick cloth.
Break the glass part of the lamp into pieces using a hammer.
Dispose of as industrial waste (except Hg/Xe burners, see above).
2.1.3 Handling Instructions and Recommendations
a The Illumination System MT10 / MT20 is a precision instrument. Handle it with great care and avoid subject-
ing it to sudden or severe impact. Also connect each cable gently.
b The built-in ventilating fan and the built-in filter wheels may generate vibrations and sound. Therefore it is
recommended not to place the Illumination System MT10 / MT20 on the same table as the microscope.
c With exception of the burner flap and the filter flap the Illumination System MT10 / MT20 may only be
opened and disassembled by authorized service personal. Otherwise warranty is lost.
d Before using the Illumination System MT10 / MT20 for the first time check that the burner is installed cor-
rectly and that all cords are connected correctly.
e Before opening the burner flap for replacement of the burner, turn it off by software (cell^R / cell^M or OBS
System Configuration) and wait for at least 30 minutes while the cooling fan is operating. Then set the main power switch on “O” (OFF) and open the burner flap. (Do not open the burner flap of the Illumination System MT10 / MT20 while the arc burner is turned on.)
f Do not place the unit inside a climate chamber.
g Do not bend the optical fiber to a diameter of less than 45 cm. The optical fiber is fragile and may brake if
bent too strongly or if torsional stress is applied.
h Cover the ends of the optical fiber with the protection cover if they are not attached to the Illumination Sys-
tem MT10 / MT20 or to the microscope.
& Hardware Manual Chapter 2 – Illumination System MT20 / MT10 11
i Always operate the Illumination System MT10 / MT20 in an upright position. Never lay it on the side during
operation. This could cause the lamp to burst, damage the equipment and cause injury.
j Before transport of the system remove the lamp and store it safely in the provided protective case.
k Mind that turning the arc burner on and off repeatedly will shorten the life span.
l To clean the surface of the Illumination System MT10 / MT20, use a lint-free, dry and soft cloth. Never at-
tempt to use organic solvents or strong detergents.
m Take care to avoid leaving fingerprints or dirt on the optical filters, the lenses at the ends of the light guide or
the optics of the illumination coupling. If contaminated, clean by wiping gently with special optical cleansing tissue. To remove fingerprints or oil stains, wipe with special optical cleansing tissue slightly moistened with lens cleansing fluid. Use the cotton gloves when handling light filters. * Since these fluids are highly inflammable, be careful to keep them away from open fire, heat or hot sur­faces and potential sources of electrical sparks, such as main switches.
2.1.4 Safety Labels
The following symbols are found on the Illumination System MT10 / MT20. Study the meaning of the symbols and always use the equipment in the safest possible manner.
Lamp housing safety label
Label Note Location
Carefully read the instructions on the label and the manual before use. Improper use could result in personal injury to the user and/or damage the equipment.
Top side of the Illumina­tion System MT20 / MT10
Fiber port safety label
Label Note Location
12 Chapter 2 – Illumination System MT20 / MT10
Status
connector
Carefully read the instructions on the label and the manual before use. Improper use could result in personal injury to the user and/or damage the equipment.
2.2 Illumination System MT10 / MT20 Overview
2.2.1 The Front Panel
Front side of the Illumination System MT20 / MT10
FIBER lock
Fiber port
LOCK
BURNER
ADJUSTMENT
Light sensor
LED:
BURNER ON
CONTROLLER
connector
LED:
AUXILIARY connector (MT20 only)
LOCK: 2 mm hex-wrench opening to lock or release the arc burner for installation and replacement.
BURNER ADJUSTMENT: three handles to align the burner.
BURNER ON LED: On / off arc burner on / off
CONTROLLER connector, MT20 only: RJ45 plug to connect the Illumination System MT20 to the cell^R Real- Time Controller.
ODB connectors (two), MT10 only: OBS Device Bus plug to connect the Illumination System MT10 to the cell^M System Coordinator.
FIBER LOCK: 1.5 mm hex-wrench opening to lock or release the optical fiber in the fiber port.
& Hardware Manual Chapter 2 – Illumination System MT20 / MT10 13
Fiber port: Opening for the optical fiber.
LIGHT SENSOR connector: 3-pin connector for light sensor cable.
AUXILIARY connector (MT20 only): 15-pin sub-D port, currently without use.
STATUS LED: Off no connection to cell^R Real-Time Controller / cell^M System Coordinator.
On active connection to cell^R Real-Time Controller / cell^M System Coordinator, ready for operation. Regular flashing Illumination System MT10 / MT20 in stand-by mode. Irregular activity Illumination System MT10 / MT20 in action.
2.2.2 Top: the Burner Flap
Before opening: Wait until system has cooled down, then switch main power off!
2.2.3 Right Side: the Filter Wheel Flap
Opening for a 3 mm hex­wrench
Access to the arc burner for replacement
The flap has to be closed and the subsequent automatic initialization has to be completed for op­eration.
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