Nihon-Kohden Celltak MEK-6400 Service manual

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Automated

Hematology Analyzer

MEK-6400/MEK-6410/MEK-6420

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www.nihonkohden.com

MEK-6400C MEK-6400J MEK-6400K

MEK-6410C MEK-6410J MEK-6410K

MEK-6420C MEK-6420J MEK-6420K

0634-900086B

CONTENTS

Contents

GENERAL HANDLING PRECAUTIONS ..............................................................................i

WARRANTY POLICY ......................................................................................................... ii

RESPONSIBILITIES – PROFESSIONAL USERS .............................................................ii

EMC Related Caution ........................................................................................................ iii

Conventions Used in this Manual and Instrument ............................................................. v

Warnings, Cautions and Notes ................................................................................ v

Section 1 General ................................................................................. 1.1

Introduction .................................................................................................................... 1.2

Service Policy ................................................................................................................ 1.3

Specifications ................................................................................................................ 1.4

Measured Parameters, Ranges and Reproducibility to Specimen from Venous

Blood .......................................................................................................... 1.4

Standardization Analysis Method ............................................................... 1.4

Detection Method ....................................................................................... 1.5

Dilution Ratio .............................................................................................. 1.5

Counting Time ............................................................................................ 1.5

Display ....................................................................................................... 1.5

Data Storage .............................................................................................. 1.5

Environmental Conditions ........................................................................... 1.5

Power Requirements .................................................................................. 1.5

Dimensions and Weight .............................................................................. 1.6

Electromagnetic Compatibility .................................................................... 1.6

Safety ........................................................................................................ 1.6

Panel Description ........................................................................................................... 1.7

Front Panel ........................................................................................................... 1.7

Right Side Panel................................................................................................... 1.8

Rear Panel ........................................................................................................... 1.9

Composition .................................................................................................................. 1.10

MEK-6400 ...........................................................................................................1.10

MEK-6410 ...........................................................................................................1.11

MEK-6420 ...........................................................................................................1.12

Interference Substances ............................................................................................... 1.13

Section 2 Troubleshooting .................................................................. 2.1

Check Procedure Flowchart ........................................................................................... 2.4

Operating Condition and Blood Sample Checks ............................................................. 2.5

Operating Condition Check ................................................................................... 2.5

Blood Sample Handling Check ............................................................................. 2.5

Pre-dilution Sample Preparation Check ................................................................ 2.5

Checking the Hematology Analyzer................................................................................ 2.7

Checking Background Noise ................................................................................ 2.7

Measurement ............................................................................................. 2.7

Service Manual MEK-6400/6410/6420 C.1

CONTENTS

Parameter Data Check with Diluent ............................................................ 2.7

Reducing Background Noise ...................................................................... 2.8

Background Noise Problems ...................................................................... 2.9

Checking the Reproducibility ................................................................................ 2.9

Checking Procedure ................................................................................... 2.9

CV Values ................................................................................................. 2.10

Checking Accuracy ....................................................................................................... 2.11

Checking Procedure ............................................................................................ 2.11

Poor WBC Reproducibility ....................................................................................2.11

Poor HGB Reproducibility ....................................................................................2.12

Poor RBC Reproducibility .................................................................................... 2.13

Poor PLT Reproducibility .....................................................................................2.14

Poor HCT or MCV Reproducibility ....................................................................... 2.15

Solving Problems from Alarm Messages ...................................................................... 2.16

A001: No diluent ........................................................................................2.16

A005: No detergent ................................................................................... 2.16

A007: No lysing reagent ............................................................................ 2.17

A009: WBC priming error ........................................................................... 2.17

A010: RBC PRIMING ERROR ................................................................... 2.17

A021: WBC level 1 .................................................................................... 2.17

A022: WBC level 2 .................................................................................... 2.18

A023: WBC level 3 .................................................................................... 2.18

A024: WBC bubble 1 ................................................................................. 2.18

A025: WBC bubble 2 ................................................................................. 2.18

A026: WBC bubble 3 ................................................................................. 2.19

A027: WBC bubble 4 ................................................................................. 2.19

A029: WBC clog ........................................................................................ 2.19

A030: WBC sample error ........................................................................... 2.19

A031: WBC noise 2 ................................................................................... 2.20

A032: WBC noise 1 ................................................................................... 2.20

A036: WBC upper manometer dirty ........................................................... 2.20

A037: WBC lower manometer dirty ............................................................ 2.20

A041: RBC level 1 .....................................................................................2.21

A042: RBC level 2 .....................................................................................2.21

A043: RBC level 3 .....................................................................................2.21

A044: RBC bubble 1 .................................................................................. 2.22

A045: RBC bubble 2 .................................................................................. 2.22

A046: RBC bubble 3 .................................................................................. 2.22

A047: RBC bubble 4 .................................................................................. 2.22

A049: RBC clog......................................................................................... 2.22

A050: RBC sample error ............................................................................2.23

A051: RBC noise 2 .................................................................................... 2.23

A052: PLT noise 1 ..................................................................................... 2.23

A053: PLT noise 3 ..................................................................................... 2.23

A057: RBC upper manometer dirty ............................................................ 2.23

A058: RBC lower manometer dirty ............................................................ 2.23

A061: HGB voltage low .............................................................................2.24

A062: HGB voltage high ............................................................................ 2.24

A063: HGB circuit error .............................................................................2.24

C.2 Service Manual MEK-6400/6410/6420

CONTENTS

A072: Tube holder open ............................................................................. 2.24

A073: Tube in the holder ............................................................................ 2.25

A091: Room temperature high ...................................................................2.25

A092: Room temperature low .................................................................... 2.25

A093: Internal temperature high ................................................................ 2.25

A094: Internal temperature low .................................................................. 2.25

A095: Power supply temp high ..................................................................2.25

! APPEARS ON THE RIGHT OF WBC MEASURED VALUE .................... 2.26

! APPEARS ON THE RIGHT OF MCHC MEASURED VALUE .................. 2.26

C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or

PLT Clumps appears ................................................................................. 2.27

OTHER FLAGS ......................................................................................... 2.27

Solving Problems from System Error Messages ........................................................... 2.28

E001: DILUTER INITIALIZE ERROR ........................................................ 2.28

E021: SAMPLER INITIALIZE ERROR ......................................................2.28

E041: SUB BATH INITIALIZE ERROR ...................................................... 2.28

E101: BATH DRAIN ERROR ..................................................................... 2.29

E122: CHECK SETTINGS ........................................................................ 2.29

E123: MEMORY ERROR .......................................................................... 2.29

E124: CIRCUIT ERROR ............................................................................ 2.29

E126: TOUCH PANEL ERROR .................................................................. 2.29

E141: CAP PIERCE INITIALIZE ERROR ................................................. 2.29

Service Maintenance Screens ....................................................................................... 2.30

Displaying the SERVICE Screen ......................................................................... 2.30

CHECK MENU Screen ........................................................................................ 2.32

UNIT MAINTE Screen ......................................................................................... 2.32

MD-640V ................................................................................................... 2.33

MS-640V ................................................................................................... 2.33

MS-641V (MEK-6400 only) ........................................................................2.34

MV & PUMP CHK ..................................................................................... 2.34

CONT MEASURE Screen ................................................................................... 2.35

SETTING MENU Screen .....................................................................................2.35

ADV. SETTINGS Screen ........................................................................... 2.35

REAGENT FACTOR Screen......................................................................2.36

USER MAINTENANCE Screen ................................................................. 2.36

PANIC VALUE Screen (for MEK-6400 and MEK-6410) .............................. 2.37

FLAG SETTINGS Screen ......................................................................... 2.37

BAR CODE PORT Screen ........................................................................ 2.37

UNIT CAL Screen ..................................................................................... 2.38

MONITOR Screen ............................................................................................... 2.38

X10-CV screen .................................................................................................... 2.39

INITIAL MENU Screen ........................................................................................2.40

OTHER MENU Screen ........................................................................................ 2.40

PRINT SETTINGS Screen .................................................................................. 2.40

Section 3 Board/Unit Description ....................................................... 3.1

Board and Unit Location ................................................................................................. 3.3

Right Side View .................................................................................................... 3.3

Service Manual MEK-6400/6410/6420 C.3

CONTENTS

Left Side View ...................................................................................................... 3.3

Front View ............................................................................................................ 3.4

Top View ............................................................................................................... 3.4

MC-640V Measuring Unit ................................................................................................ 3.5

MD-640V Combination Syringe Pump Unit ..................................................................... 3.5

MP-640V Pump Unit ....................................................................................................... 3.6

PV-641V Front Panel Unit ............................................................................................... 3.6

JQ-640V Inlet/Outlet Unit ............................................................................................... 3.7

JQ-641V/JQ-642V Valve Unit ......................................................................................... 3.7

JQ-641V Valve Unit .............................................................................................. 3.7

JQ-642V Valve Unit .............................................................................................. 3.7

MS-640V Sampler Unit ................................................................................................... 3.8

MS-641V Cap Pierce Unit (MEK-6400 only) ................................................................... 3.8

MR-640V Rinse Unit (MEK-6410/6420 only) ................................................................... 3.9

UT-7192 AMP CONTROL Board ..................................................................................... 3.9

UT-7193 POWER Board ................................................................................................ 3.10

UT-7198 MEASURING Board ........................................................................................ 3.10

UT-7201 HGB AMP Board and UT-7202 HGB LED Board .............................................. 3.11

UT-7200 MIXED PUMP Board ....................................................................................... 3.11

UT-7205 PRINTER DRIVER Board ................................................................................ 3.12

UT-7203 KEY Board ...................................................................................................... 3.12

UT-7199 LIQUID SENSOR Board .................................................................................. 3.13

XP-602V/XP-612V 2-way Electromagentic Valve .......................................................... 3.13

Section 4 Disassembly and Assembly .............................................. 4.1

Before You Begin ............................................................................................................ 4.2

Warnings and Cautions ......................................................................................... 4.2

Required Tools ...................................................................................................... 4.2

Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the

Instrument ............................................................................................................ 4.3

Board and Unit Location ....................................................................................... 4.3

Turning the Power Off ..................................................................................................... 4.4

Cleaning and Draining the Fluid Pathway .............................................................. 4.4

Turning the Power Off ........................................................................................... 4.5

Removing the Right Side Cover, Top Cover and Rear Cover........................................... 4.6

Reattaching the Right Side Cover, Top Cover and Rear Cover ............................. 4.7

Removing the Front Panel Unit ....................................................................................... 4.8

Reattaching the Front Panel Unit .......................................................................... 4.9

Installing the WA-640VK Printer Unit (Option)................................................................4.10

Removing the Measuring Unit ....................................................................................... 4.12

Reattaching the Measuring Unit ..........................................................................4.13

Accessing the Connectors on the POWER Board .........................................................4.14

Reassembling the Board .....................................................................................4.14

Removing the Combination Syringe Pump Unit .............................................................4.15

Reattaching the Combination Syringe Pump Unit ................................................4.16

Removing the Pump Unit ............................................................................................... 4.17

Reattaching the Pump Unit ................................................................................. 4.18

Removing the Sampler Unit ........................................................................................... 4.19

C.4 Service Manual MEK-6400/6410/6420

CONTENTS

Reattaching the Sampler Unit..............................................................................4.22

Removing the Cap Pierce Unit or Rinse Unit .................................................................4.23

Reattaching the Cap Pierce Unit or Rinse Unit .................................................... 4.24

Removing the Inlet/Outlet Unit ...................................................................................... 4.25

Reattaching the Inlet/Outlet Unit ......................................................................... 4.26

Removing the Valve Unit ............................................................................................... 4.26

Reattaching the Valve Unit .................................................................................. 4.27

Removing the AMP CONTROL Board ........................................................................... 4.28

Reattaching the AMP CONTROL Board .............................................................. 4.29

Removing the POWER Board ........................................................................................ 4.30

Reattaching the POWER Board ..........................................................................4.31

Removing the MEASURING Board ...............................................................................4.32

Reattaching the MEASURING Board .................................................................. 4.33

Removing the MIXED PUMP Board ..............................................................................4.34

Reattaching the MIXED PUMP Board .................................................................4.34

Removing the PRINTER DRIVER Board ....................................................................... 4.35

Reattaching the PRINTER DRIVER Board .......................................................... 4.35

Removing the KEY Board..............................................................................................4.36

Reattaching the KEY Board ................................................................................ 4.36

Removing the LIQUID SENSOR Board ......................................................................... 4.37

Reattaching the LIQUID SENSOR Board ............................................................ 4.37

Removing the LCD ........................................................................................................ 4.38

Reattaching the LCD ........................................................................................... 4.39

Removing the Transformer .............................................................................................4.40

Reattaching the Transformer ................................................................................ 4.40

Section 5 Adjustment........................................................................... 5.1

General ........................................................................................................................... 5.2

Adjusting the HGB Sensor Output Voltage ..................................................................... 5.3

Adjusting the Upper and Lower Sensor Output Voltages of the Manometers .................. 5.6

Adjusting the Liquid Sensor Output Voltages .................................................................. 5.9

Section 6 Maintenance ........................................................................ 6.1

General ........................................................................................................................... 6.3

Disposing of Waste............................................................................................... 6.4

Repair Parts Availability Policy ............................................................................. 6.4

Parts to be Replaced Periodically......................................................................... 6.4

Maintenance Schedule ................................................................................................... 6.5

Displaying Operation History Screen .............................................................................. 6.6

Maintenance Check Sheet ............................................................................................. 6.8

Before Maintenance Procedure ..................................................................................... 6.12

Strong Cleaning ...................................................................................................6.12

Draining Measurement Baths and Sub Baths ...................................................... 6.13

Turning Power Off ................................................................................................ 6.14

Daily Maintenance Procedures ......................................................................................6.15

Checking Reagents and Other Consumables ...................................................... 6.15

Checking the Appearance of the Analyzer .......................................................... 6.15

Service Manual MEK-6400/6410/6420 C.5

CONTENTS

Disinfecting the Surface of the Analyzer ................................................... 6.16

Cleaning the Surface of the Analyzer ........................................................6.16

Checking the Reagents Connection Tubes .......................................................... 6.16

Checking the Power Cord and Grounding Lead....................................................6.16

Checking the External Instrument Connection ....................................................6.16

Checking the Power On ....................................................................................... 6.17

Calibrating the Touch Screen ..................................................................... 6.17

Checking the Date and Time ............................................................................... 6.17

Clock Accuracy.........................................................................................6.18

Measuring Background Noise..............................................................................6.18

Measuring Hematology Control ........................................................................... 6.20

Checking Measurement Baths and Sub Baths .................................................... 6.21

Checking Pump Tube........................................................................................... 6.21

Weekly/Every 300 Counts Maintenance Procedures ..................................................... 6.21

Checking/Cleaning Filters ................................................................................... 6.21

Monthly/Every 1,000 Counts Maintenance Procedures ................................................. 6.22

Replacing Filters ................................................................................................. 6.22

Materials Required ....................................................................................6.22

Procedure ................................................................................................. 6.22

Checking and Cleaning Measurement Baths and Sub Baths ............................... 6.23

Materials Required ....................................................................................6.23

Procedure ................................................................................................. 6.24

Checking and Cleaning/Replacing the Rinse Unit and Cap Pierce Nozzle ...........6.26

Materials Required ....................................................................................6.26

Procedure ................................................................................................. 6.27

Every Four Months/Every 3,000 Counts Maintenance Procedures ............................... 6.29

Checking and Cleaning/Replacing the Sampling Nozzles .................................... 6.29

Materials Required ....................................................................................6.29

Procedure ................................................................................................. 6.29

Replacing Pump Tube ..........................................................................................6.32

Materials Required ....................................................................................6.32

Procedure ................................................................................................. 6.32

Every Six Months/As-Required Maintenance Procedures .............................................6.35

Removing a Clog from the Aperture .................................................................... 6.35

Cleaning Aperture Caps ...................................................................................... 6.36

Materials Required ....................................................................................6.36

Procedures................................................................................................6.36

Cleaning the External Electrodes on the Measurement Baths ............................ 6.39

Checking the Sensor Monitor Screen .................................................................. 6.40

Checking the Circuit ............................................................................................ 6.41

Checking the X_-R Values .....................................................................................6.43

Checking the Current Calibration Coefficients ..................................................... 6.43

Checking the New Calibration Coefficients .......................................................... 6.43

Checking the Prime Function .............................................................................. 6.44

Checking the Drain Function ............................................................................... 6.44

Checking the Cleaning Function .......................................................................... 6.44

Checking the Dispensing Function ...................................................................... 6.45

Checking the External Instruments Function ...................................................... 6.45

Printers ..................................................................................................... 6.45

C.6 Service Manual MEK-6400/6410/6420

CONTENTS

Hand-held Bar Code Reader ......................................................................6.45

PC.............................................................................................................6.45

Checking the Power Cord ....................................................................................6.45

Checking the Resistance of the Protective Ground Line ..................................... 6.45

Checking the Earth Leakage Current ................................................................... 6.46

Section 7 Test Point, Variable Resistor, LED and Switch on Board 7.1

AMP CONTROL Board ................................................................................................... 7.2

POWER Board ............................................................................................................... 7.4

MEASURING Board ....................................................................................................... 7.5

HGB AMP Board ............................................................................................................ 7.6

LIQUID SENSOR Board ................................................................................................. 7.6

MIXED PUMP Board ...................................................................................................... 7.7

KEY Board ..................................................................................................................... 7.7

PRINTER DRIVER Board ............................................................................................... 7.8

Section 8 Socket Pin Assignment...................................................... 8.1

ZK-820V Bar Code Reader Socket ....................................................................... 8.2

Serial Port 1/Serial Port 2 .................................................................................... 8.2

Printer Socket ...................................................................................................... 8.2

USB Socket ......................................................................................................... 8.2

Section 9 Installation............................................................................ 9.1

Connecting the Power Cord and Grounding the Analyzer ................................................ 9.2

Connecting the Power Cord .................................................................................. 9.2

Equipotential Grounding ....................................................................................... 9.2

Connecting Tubes and Installing Reagents ..................................................................... 9.3

Materials Required ............................................................................................... 9.3

Connecting Tubes ................................................................................................. 9.4

Diluent Tube ............................................................................................... 9.4

Detergent Tube ........................................................................................... 9.5

Lysing Reagent Tube .................................................................................. 9.6

Waste Fluid Tube ........................................................................................ 9.6

Turning Power On/Off ..................................................................................................... 9.7

Turning On the Power ........................................................................................... 9.7

Cleaning the Fluid Path After Turning the Power On (PRIME ON INSTALL) .......... 9.8

Turning Off the Power ........................................................................................... 9.9

Checking Accuracy ....................................................................................................... 9.10

Checking the Circuit ............................................................................................9.10

Measuring Background Noise..............................................................................9.10

Calibrating ...........................................................................................................9.11

Service Manual MEK-6400/6410/6420 C.7

GENERAL HANDLING PRECAUTIONS

This device is intended for use only by qualified medical personnel. Use only Nihon Kohden approved products with this device. Use of non-approved products or in a non-approved manner may affect the performance specifications of the device. This includes, but is not limited to, batteries, recording paper, pens, extension cables, electrode leads, input boxes and AC power.

Please read these precautions thoroughly before attempting to operate the instrument.

1. To safely and effectively use the instrument, its operation must be fully understood.

2. When installing or storing the instrument, take the following precautions:

(1) Avoid moisture or contact with water, extreme atmospheric pressure, excessive humidity and temperatures,

poorly ventilated areas, and dust, saline or sulphuric air.

(2) Place the instrument on an even, level floor. Avoid vibration and mechanical shock, even during transport.

(3) Avoid placing in an area where chemicals are stored or where there is danger of gas leakage.

(4) The power line source to be applied to the instrument must correspond in frequency and voltage to product

specifications, and have sufficient current capacity.

(5) Choose a room where a proper grounding facility is available.

3. Before Operation

(1) Check that the instrument is in perfect operating order.

(2) Check that the instrument is grounded properly.

(3) Check that all cords are connected properly.

(4) Pay extra attention when the instrument is in combination with other instruments to avoid misdiagnosis or other

problems.

(5) All circuitry used for direct patient connection must be doubly checked.

(6) Check that battery level is acceptable and battery condition is good when using battery-operated models.

4. During Operation

(1) Both the instrument and the patient must receive continual, careful attention.

(2) Turn power off or remove electrodes and/or transducers when necessary to assure the patient’s safety.

(3) Avoid direct contact between the instrument housing and the patient.

5. To Shutdown After Use

(1) Turn power off with all controls returned to their original positions.

(2) Remove the cords gently; do not use force to remove them.

(3) Clean the instrument together with all accessories for their next use.

6. The instrument must receive expert, professional attention for maintenance and repairs. When the instrument is

not functioning properly, it should be clearly marked to avoid operation while it is out of order.

7. The instrument must not be altered or modified in any way.

8. Maintenance and Inspection

(1) The instrument and parts must undergo regular maintenance inspection at least every 6 months.

(2) If stored for extended periods without being used, make sure prior to operation that the instrument is in perfect

operating condition.

Service Manual MEK-6400/6410/6420 i

9. When the instrument is used with an electrosurgical instrument, pay careful attention to the application and/or

location of electrodes and/or transducers to avoid possible burn to the patient.

10. When the instrument is used with a defibrillator, make sure that the instrument is protected against defibrillator

discharge. If not, remove patient cables and/or transducers from the instrument to avoid possible damage.

WARRANTY POLICY

Nihon Kohden Corporation (NKC) shall warrant its products against all defects in materials and workmanship for one

year from the date of delivery. However, consumable materials such as recording paper, ink, stylus and battery are

excluded from the warranty.

NKC or its authorized agents will repair or replace any products which prove to be defective during the warranty period,

provided these products are used as prescribed by the operating instructions given in the operator’s and service manuals.

No other party is authorized to make any warranty or assume liability for NKC’s products. NKC will not recognize any

other warranty, either implied or in writing. In addition, service, technical modification or any other product change

performed by someone other than NKC or its authorized agents without prior consent of NKC may be cause for voiding

this warranty.

Defective products or parts must be returned to NKC or its authorized agents, along with an explanation of the failure.

Shipping costs must be pre-paid.

This warranty does not apply to products that have been modified, disassembled, reinstalled or repaired without Nihon

Kohden approval or which have been subjected to neglect or accident, damage due to accident, fire, lightning,

vandalism, water or other casualty, improper installation or application, or on which the original identification marks

have been removed.

In the USA and Canada other warranty policies may apply.

RESPONSIBILITIES – PROFESSIONAL USERS

This instrument must be used by a professional user with a full knowledge of operating this instrument, only for his/her

intended use and according to the instructions for use. Instructions in the operator’s manual must be followed, especially

the following points.

• Storage and stability of reagents

• Handling of reagents

• Instrument installation

• Connection of all tubes to inlets and outlets

• Connection of all tubes to reagents and waste container

• Checking the amount of reagents and waste fluid

• Calibration

• Quality control

• Maintaining and servicing

If deviating from the instructions, the professional user does it at the risk and liability of the laboratory and only after

validation by the laboratory. Nihon Kohden has no responsibility over such deviations.

ii Service Manual MEK-6400/6410/6420

EMC RELATED CAUTION

This equipment and/or system complies with the International Standard EN61326-1 for electromagnetic

compatibility for electrical equipment and/or system for measurement, control and laboratory use.

However, an electromagnetic environment that exceeds the limits or levels stipulated in the EN61326-1,

can cause harmful interference to the equipment and/or system or cause the equipment and/or system to

fail to perform its intended function or degrade its intended performance. Therefore, during the operation

of the equipment and/or system, if there is any undesired deviation from its intended operational

performance, you must avoid, identify and resolve the adverse electromagnetic effect before continuing

to use the equipment and/or system.

The following describes some common interference sources and remedial actions:

1. Strong electromagnetic interference from a nearby emitter source such as an authorized radio station

or cellular phone:

Install the equipment and/or system at another location if it is interfered with by an emitter source

such as an authorized radio station. Keep the emitter source such as cellular phone away from the

equipment and/or system.

2. Radio-frequency interference from other equipment through the AC power supply of the equipment

and/or system:

Identify the cause of this interference and if possible remove this interference source. If this is not

possible, use a different power supply.

3. Effect of direct or indirect electrostatic discharge:

Make sure all users and patients in contact with the equipment and/or system are free from direct or

indirect electrostatic energy before using it. A humid room can help lessen this problem.

4. Electromagnetic interference with any radio wave receiver such as radio or television:

If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/or

system as far as possible from the radio wave receiver.

If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden

Corporation subsidiary or distributor for additional suggestions.

This equipment complies with International Standard EN55011 (1999) Group 1, Class B. Class B

EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly

connected to a low voltage power supply network which supplies buildings used for domestic purposes.

The CE mark is a protected conformity mark of the European Community. The products herewith comply

with the requirements of the IVD Directive 98/79/EEC.

Service Manual MEK-6400/6410/6420 iii

NOTE about Waste Electrical and Electronic Equipment (WEEE) directive 2002/96/EEC

For the member states of the European Union only:

The purpose of WEEE directive 2002/96/EEC is, as a first priority, the prevention of waste electrical and

electronic equipment (WEEE), and in addition, the reuse, recycling and other forms of recovery of such

wastes so as to reduce the disposal of waste.

Contact your Nihon Kohden representative for disposal at the end of its working life.

iv Service Manual MEK-6400/6410/6420

Conventions Used in this Manual and Instrument

Warnings, Cautions and Notes

Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.

WARNING

A warning alerts the user to the possible injury or death associated with the use or misuse of the

instrument.

CAUTION

A caution alerts the user to possible injury or problems with the instrument associated with its use or

misuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to other

property.

NOTE

A note provides specific information, in the form of recommendations, prerequirements, alternative

methods or supplemental information.

Service Manual MEK-6400/6410/6420 v

This page is intentionally left blank.

vi Service Manual MEK-6400/6410/6420

Section 1 General

Introduction ........................................................................................................................................................ 1.2

Service Policy .................................................................................................................................................... 1.3

Specifications .................................................................................................................................................... 1.4

Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood ..................... 1.4

Standardization Analysis Method ................................................................................................... 1.4

Detection Method ........................................................................................................................... 1.5

Dilution Ratio ................................................................................................................................. 1.5

Counting Time ................................................................................................................................ 1.5

Display .......................................................................................................................................... 1.5

Data Storage .................................................................................................................................. 1.5

Environmental Conditions .............................................................................................................. 1.5

Power Requirements ...................................................................................................................... 1.5

Dimensions and Weight ................................................................................................................. 1.6

Electromagnetic Compatibility ........................................................................................................ 1.6

Safety ............................................................................................................................................ 1.6

Panel Description ............................................................................................................................................... 1.7

Front Panel ............................................................................................................................................... 1.7

Right Side Panel ...................................................................................................................................... 1.8

Rear Panel ............................................................................................................................................... 1.9

Composition ......................................................................................................................................................1.10

MEK-6400 ............................................................................................................................................... 1.10

MEK-6410 ............................................................................................................................................... 1.11

MEK-6420 ............................................................................................................................................... 1.12

Interference Substances ................................................................................................................................... 1.13

Service Manual MEK-6400/6410/6420 1.1

1. GENERAL

Introduction

CAUTION

To maintain the instrument in normal condition, the user must

perform the periodic maintenance. Refer to “Maintenance” of the

operator’s manual.

This service manual provides useful information to qualified service personnel

to understand, troubleshoot, service, maintain and repair the MEK-6400/6410/

6420 Automated Hematology Analyzer (referred to as “the instrument” in this

service manual).

The maintenance must be periodically performed because the instrument has

fluid paths and precision parts. Accordingly, the user is responsible for

performing the periodic maintenance. The “Maintenance” section in this

service manual describes the maintenance that should be performed by

qualified service personnel. The “Maintenance” section in the operator’s

manual describes the maintenance that can be performed by the user.

NOTE

If the instrument has a problem and there has been no periodic

maintenance, the instrument will usually be normal again by

cleaning the fluid paths or replacing a consumable with a new one.

The information in the operator’s manual is primarily for the user. However, it

is important for service personnel to thoroughly read the operator’s manual

and service manual before starting to troubleshoot, service, maintain or repair

this instrument. This is because service personnel needs to understand the

operation of the instrument in order to effectively use the information in the

service manual.

For simplicity, the suffix C/J/K will be omitted in this manual. There is no

difference in operation and servicing among models with different suffixes

unless otherwise specified.

1.2 Service Manual MEK-6400/6410/6420

1. GENERAL

Service Policy

CAUTION

• Be careful not to directly touch any place where blood is or may

spread to.

• Wear rubber gloves to protect yourself from infection before doing

maintenance.

Nihon Kohden Corporation’s basic policy for technical service is to replace

faulty units, printed circuit boards or parts. We do not support component-

level repair of boards and units outside the factory.

NOTE

• When ordering parts or accessories from your nearest Nihon

Kohden Corporation’s representative, please quote the NK code

number and part name which is listed in this service manual, and

the name or model of the unit in which the required part is located.

This will help us to promptly attend to your needs.

• Always use parts and accessories recommended or supplied by

Nihon Kohden Corporation to assure maximum performance from

your instrument.

Service Manual MEK-6400/6410/6420 1.3

1. GENERAL

Specifications

Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood

Specifications except WBC population were determined using hematology control blood (MEK-3DN), counted 10 times

consecutively.

Reproducibility to Specimen from

Measured Parameters Measuring Range

(CV: Coefficient of Variation)

WBC: White blood cell count

LY%: Lymphocyte percent 0 to 99.9%

MO%: Monocyte percent 0 to 99.9%

GR%: Granulocyte percent 0 to 99.9%

LY: Lymphocyte count

MO: Monocyte count

GR: Granulocyte count

RBC: Red blood cell count 0 to 14.9 × 106/µL within 1.5%CV (5.0 × 106/µL)

HGB: Hemoglobin concentration 0 to 29.9 g/dL within 1.5%CV (16 g/dL)

HCT: Hematocrit 0 to 99.9%

MCV: Mean cell volume 20 to 199 fL within 1.0%CV (70 to 120 fL)

MCH: Mean cell hemoglobin 10 to 50 pg

MCHC: Mean cell hemoglobin concentration RDW: Red blood cell distribution width

PLT: Platelet count 0 to 1490 × 103/µL within 4.0%CV (3.0 × 103/µL)

PCT: Platelet crit 0 to 2.9%

MPV: Mean platelet volume 0 to 20.0 fL

PDW: Platelet distribution width 0 to 50%

MEK-6400/6410: 0 to 59 × 103/µL* MEK-6420: 0 to 99 × 103/µL

MEK-6400/6410: 0 to 59 × 103/µL* MEK-6420: 0 to 99 × 103/µL MEK-6400/6410: 0 to 59 × 103/µL* MEK-6420: 0 to 99 × 103/µL MEK-6400/6410: 0 to 59 × 103/µL* MEK-6420: 0 to 99 × 103/µL

10 to 50 g/dL

0 to 50%

within 2.0%CV (4.0 to 9.0 × 103/µL)

within 5.0%CV (WBC: 4.0 to 9.0 × 103/µL, LY%: 20 to 45%) within 12.0%CV (WBC: 4.0 to 9.0

/µL, MO%: 2 to 10%) within 5.0%CV (WBC: 4.0 to 9.0 × 103/µL, GR%: 40 to 70%)

Venous Blood

* In panic value recount: 0 to 599 × 103/µL (MEK-6400, MEK-6410 only)

Standardization Analysis Method

WBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood

cell counters. Clin Lab Haematol, 10:203-212, 1988

RBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood

cell counters. Clin Lab Haematol, 10:203-212, 1988

HGB: NCCLS H15-A2 H15-A2: Reference and Selected Procedures for the Quantitative Determination of

Hemoglobin in Blood Second Edition; Approved Standard (1994)

HCT: NCCLS H7-A2 H7-A2: Procedure for Determining Packed Cell Volume by the Microhematocrit

Method Second Edition; Approved Standard (1993)

PLT: Brecher & Cronkite Method: Morphology and enumeration of human blood platelets, J Appl Physiol 3 365 (Dec)

1950; Brecher G, Cronkite EP

1.4 Service Manual MEK-6400/6410/6420

1. GENERAL

Detection Method

Blood cell count: Electrical resistance detection

Hemoglobin: Surfactant method (colorimetric method)

Hematocrit: Histogram calculation

WBC population: Histogram calculation

Platelet crit: Histogram calculation

RBC distribution width: Histogram calculation

Platelet distribution width: Histogram calculation

Dilution Ratio

• Venous blood

Sample volume: 30 µL in normal dilution mode, about 50 µL in low dilution mode, 10 µL in high dilution mode,

5 µL in higher dilution mode (low and higher dilution modes are not available on the MEK-6420

analyzer)

WBC/HGB: 200:1 (in normal dilution mode)

RBC/PLT: 40,000:1 (in normal dilution mode)

• Pre-dilution blood

Sample volume: 10 µL 20 µL

WBC/HGB: 1200:1 600:1

RBC/PLT: 240,000:1 120,000:1

Counting Time

Closed mode: about 90 s/sample (from measurement start to data display, MEK-6400 only)

Open mode: about 60 s/sample (from measurement start to data display)

Display

Display: 5.7 inch, color LCD with backlight and touch screen keys

Resolution: 240 × 320 dots

Screen size: approx. 86 × 115 mm

Display contents: Numerical data, histograms, measuring conditions, alarm message and other messages, touch screen

keys

Data Storage

Numerical data for all counted parameters for up to 400 samples and histograms for up to 50 samples

Environmental Conditions

Storage temperature: –20 to 60°C

Operating temperature: 15 to 30°C

Storage humidity: 10 to 95%

Operating humidity: 30 to 85% (Non-condensing)

Storage atmospheric pressure: 70 to 106 kPa

Operating atmospheric pressure: 70 to 106 kPa

Operating altitude: less than 3000 m

Power Requirements

Power requirements: MEK-6400J, 6410J, 6420J: 110 to 127 V ±10% AC, 50/60 Hz

MEK-6400C/K, 6410C/K, 6420C/K: 220 to 240 V ±10% AC, 50/60 Hz

Power consumption:less than120 VA

Service Manual MEK-6400/6410/6420 1.5

1. GENERAL

Dimensions and Weight

Dimensions: 230 W × 450 D × 383 H (mm)

Net weight: approx. 18 kg

Electromagnetic Compatibility

IEC 61326-1 Edition 1.0: 2002 (Annex A)

EN 61326: 1997/ Amendment 3: 2003

CISPR11 Edition 4.1: 2004, Group 1, Class B

EN 55011: 1998 Amendment 1: 1999, Group 1, Class B

The power supply short interruption test is performed through the transformer which has at least three times the power

capacity as the instrument.

Safety

Safety standards: IEC 61010-1 Edition 2.0: 2001

EN 61010-1: 2001

IEC 61010-2-101: 2002

EN 61010-2-101: 2002

IEC 61010-2-081: 2002

EN 61010-2-081: 2002

According to the type of protection against electrical shock: CLASS I EQUIPMENT

According to the degree of protection against harmful ingress of water: IPX0 (Ordinary EQUIPMENT)

According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH

AIR, OR WITH OXYGEN OR NITROUS OXIDE: EQUIPMENT not suitable for use in the presence of FLAMMABLE

ANAESTHETIC MIXTURE WITH AIR, OR WITH OXYGEN OR

NITROUS OXIDE

According to the mode of operation: CONTINUOUS OPERATION

EQUIPMENT types (classification): Indoor stationary EQUIPMENT

Pollution Degree: 2 EQUIPMENT

Requirements for marking of IN VITRO DIAGNOSTIC instruments: EN1658: 1996

1.6 Service Manual MEK-6400/6410/6420

1. GENERAL

Panel Description

Front Panel

4 5 6

MEK-6400

MEK-6410/6420

No. Name Description

1 Main power lamp Lights when the [Main power] switch on the rear panel is tuned on.

2Power lamp

Lights when the [Main power] switch on the rear panel and [Power] key on the front panel are turned on. Turns the analyzer power on or off when the [Main power] switch on the rear panel is turned

3Power key

on. When the power is turned on, priming and self-check are automatically performed, and the READY screen appears.

4 Auto print key Switches the printing mode between automatic and manual for the printer.

5 Feed key Feeds paper of the printer while held down.

6 Print key Prints displayed data on the printer.

Auto print mode

lamp

Lights when automatic printing mode is selected.

8 LCD display Displays various messages, measured data and touch screen keys.

9 Reset key

Stops operation when pressed during operation. Returns to the READY screen when pressed while changing settings. Use this key only when an error occurs. Cleans the fluid path, aperture and manometer with detergent. Automatically primes after

10 Clean key

cleaning the fluid path. Press this key when clogging occurs, the manometer becomes dirty or bubbles occur in the manometer.

Eject key For closed mode only. Opens the tube holder to set the sample tube. (MEK-6400)

Count key

Dispense key

12 Tube holder

13 Sampling nozzle

Aspirates the sample and starts counting when <[Eject] key operation> is set to “Count” on the OPERATION screen of the SETTINGS screen. (MEK-6410, MEK-6420)11 Dispenses the diluent in pre-dilution blood mode when <[Eject] key operation > is set to “Dispense” on the OPERATION screen of the SETTINGS screen. (MEK-6410, MEK-6420) For closed mode only. Holds a sealed vacuum blood collecting tube. Press the [Eject] key to open. After measurement, the holder automatically opens. (MEK-6400) Aspirates the sample. Dispenses the diluent when in the pre-dilution blood mode. (On MEK6400, for open mode only)

14 Count switch Aspirates the sample and starts counting.

Printer unit

(WA-640VK)

16 Printer door

Thermal array printer. Prints out measured data and sample ID no. (optional)

For the recording paper of the WA-640VK printer unit. To open, pull the upper left corner (optional).

Service Manual MEK-6400/6410/6420 1.7

1. GENERAL

Right Side Panel

No. Name Description

ISO3

Diluent inlet CLN

Detergent inlet HEMO3N

Hemolysing reagent inlet WA ST E

Waste outlet

Inlet for the ISOTONAC•3 diluent.

Inlet for the CLEANAC detergent.

Inlet for the Hemolynac•3N hemolysing reagent.

Outlet for waste such as used lyse, detergent and aspirated samples.

1.8 Service Manual MEK-6400/6410/6420

1. GENERAL

Rear Panel

1 2 3

No. Name Description

1 Main power switch

2 Fuse holder

AC SOURCE

AC source socket Equipotential ground

terminal

ZK-820V

Bar code reader socket

6 Serial port 1 Connects to the optional WA-460V/461V card printer or PC.

7 Serial port 2 Connects to the optional WA-460V/461V card printer or PC.

8 Printer socket Connects to an external printer (WA-710V/712V or other).

9 USB socket

10 Memory card socket Insert a memory card when you upgrade the software.

11 Memory card eject button Press this button when you eject a memory card.

Supplies the power to the analyzer when it is turned on. Under normal conditions keep this switch turned on. Contains the time lag fuse. To replace the fuse, contact your Nihon Kohden representative.

Connects the AC power cord to supply AC power to the analyzer.

Connects the ground lead to the equipotential ground terminal on the wall for earth grounding. Connects to the optional hand-held bar code reader. Supplies the power to the bar code reader when connected. Power supply voltage: 5 V DC (pin 9: 5 V, pin 5: GND) Rated current: 200 mA

Connects to a PC. The optional Data Management Software needs to be installed on the PC to receive data from the analyzer.

Service Manual MEK-6400/6410/6420 1.9

1. GENERAL

Composition

MEK-6400

Standard

MEK-6400J MEK-6400K MEK-6400C

MC-640V Measuring Unit

UT-7198 MEASURING Board

UT-7201 HGB AMP Board

UT-7202 HGB LED Board

XP-612V 2-Way Electromagnetic Value (× 10)

XP-602V 2-Way Electromagnetic Value (× 2)

MD-640V Combination Syringe Pump Unit

UT-7200 MIXED PUMP Board

XP-612V 2-Way Electromagnetic Value (× 4)

MP-640V Pump Unit

PV-641VK Front Panel Unit

UT-7203 KEY Board

JQ-640V Inlet/Outlet Unit

UT-7199 LIQUID SENSOR Board

XP-612V 2-Way Electromagnetic Value (× 2)

JQ-641V Valve Unit

XP-612V 2-Way Electromagnetic Value (× 6)

MS-640V Sampler Unit

MS-641V Cap Pierce Unit

UT-7192 AMP CONTROL Board

UT-7193 POWER Board

Option

WA-640VK Printer Unit

UT-7205 PRINTER DRIVER Board

1.10 Service Manual MEK-6400/6410/6420

1. GENERAL

MEK-6410

Standard

MEK-6410J MEK-6410K MEK-6410C

MC-640V Measuring Unit

UT-7198 MEASURING Board

UT-7201 HGB AMP Board

UT-7202 HGB LED Board

XP-612V 2-Way Electromagnetic Value (× 10)

XP-602V 2-Way Electromagnetic Value (× 2)

MD-640V Combination Syringe Pump Unit

UT-7200 MIXED PUMP Board

XP-612V 2-Way Electromagnetic Value (× 4)

MP-640V Pump Unit

PV-641VK Front Panel Unit

UT-7203 KEY Board

JQ-640V Inlet/Outlet Unit

UT-7199 LIQUID SENSOR Board

XP-612V 2-Way Electromagnetic Value (× 2)

JQ-642V Valve Unit

XP-612V 2-Way Electromagnetic Value (× 4)

MS-640V Sampler Unit

MR-640V Rinse Unit

UT-71921 AMP CONTROL Board

UT-7193 POWER Board

Option

WA-640VK Printer Unit

UT-7205 PRINTER DRIVER Board

Service Manual MEK-6400/6410/6420 1.11

1. GENERAL

MEK-6420

Standard

MEK-6420J MEK-6420K MEK-6420C

MC-640V Measuring Unit

UT-7198 MEASURING Board

UT-7201 HGB AMP Board

UT-7202 HGB LED Board

XP-612V 2-Way Electromagnetic Value (x 10)

XP-602V 2-Way Electromagnetic Value (x 2)

MD-640V Combination Syringe Pump Unit

UT-7200 MIXED PUMP Board

XP-612V 2-Way Electromagnetic Value (x 4)

MP-640V Pump Unit

PV-641VK Front Panel Unit

UT-7203 KEY Board

JQ-640V Inlet/Outlet Unit

UT-7199 LIQUID SENSOR Board

XP-612V 2-Way Electromagnetic Value (x 2)

JQ-642V Valve Unit

XP-612V 2-Way Electromagnetic Value (x 4)

MS-640V Sampler Unit

MR-640V Rinse Unit

UT-71922 AMP CONTROL Board (MEK-6420J/K)

UT-71923 AMP CONTROL Board (MEK-6420C)

UT-7193 POWER Board

Option

WA-640VK Printer Unit

UT-7205 PRINTER DRIVER Board

1.12 Service Manual MEK-6400/6410/6420

1. GENERAL

Interference Substances

WBC: High WBC

When WBC count is outside the measurable range, measure the sample in high dilution mode. If the WBC

count is still outside the range, dilute the sample further.

Nucleated erythrocyte

Nucleated erythrocyte is detected as WBC and causes increase in WBC count.

Unlysed red cells

In some rare occasions, the RBC in the blood sample may not completely lyse and these non-lysed RBC may

be detected as WBC and cause increase in WBC count.

Multiple myeloma

The precipitation of proteins in multiple myeloma patients may increase the WBC count.

Leukemia

WBC is fragile in leukemia patients and WBC may be destroyed during measurement. These WBC fragments

may also interfere with WBC differential measurement.

Chemotherapy

Cytotoxic and immunosuppressive drugs cause low WBC count.

Cryoglobulins

Cryoglobulin may be increased in patients who are pregnant or have myeloma, cancer, leukemia,

macroglobulinemia, lymphoproliferative disorders, metastatic tumors, autoimmune disorders, infections,

aneurysm, thromboembolic phenomena, diabetes, etc, which cause increase in WBC, RBC or PLT counts and

HGB concentration. In such cases, warm the blood sample to 37°C in a water bath for 30 minutes and measure

the sample immediately.

RBC: Leukemia

An increase in WBC in leukemia patient causes increase in RBC.

Agglutinated RBC

Agglutinated RBC may decrease RBC count. This can be checked by abnormal MCH and MCHC values and

examination of the stained blood film.

Cold agglutinins

IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts and

increase MCV.

Hemolysis

When RBC is hemolyzed, RBC is decreased.

HGB: Turbidity of the blood sample

Any physiologic and/or therapeutic factors may increase HGB. In such a case, determine the cause of turbidity

and follow the appropriate method below.

1. Increased WBC

An extreme increase in WBC causes excessive light scatter. In these cases, measure manually. Centrifuge

the diluted sample and measure the supernatant fluid with a spectrophotometer.

2. Increased lipids

The blood sample may be milky when there is excessive lipids. This may occur with hyperlipidemia,

hyperproteinemia and hyperbilirubinemia. Accurate HGB measurement can be achieved by manual

methods and a plasma blank.

3. Increased turbidity

When RBC are resistant to lysing, turbidity may increase causing increase in HGB. Observe if MCH and

MCHC values are abnormal. HGB result affects MCH and MCHC result.

4. Fetal bloods

The mixing of fetal and maternal blood may increase HGB value.

Service Manual MEK-6400/6410/6420 1.13

1. GENERAL

5. High WBC levels

Turbidity of blood increases and the hemoglobin concentration becomes high if WBC level of the blood

sample is high. MCH and MCHC levels also become high.

HCT: Agglutinated RBC

RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are

abnormal. In such a case, measure manually.

MCV: Agglutinated RBC

RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are

abnormal. In such a case, measure manually.

Excessive number of large PLT

Excessive number of large PLT and/or excessively high WBC may affect the MCV value. Check by careful

examination of the stained blood film.

MCH: MCH is determined from HGB and RBC values. Therefore, the limitations for HGB and RBC also affect MCH

value.

MCHC: MCHC is determined from HGB and HCT values. Therefore, the limitations for HGB and HCT also affect

MCHC value.

RDW: RDW is determined from RBC value. Therefore, the limitations for RBC also affect RDW value.

Agglutinated RBC

Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked by abnormal MCH and

MCHC values and examination of the stained blood film.

Nutritional deficiency or blood transfusion

Iron and/or cobalamin and/or folate deficiency may increase RDW.

PLT: Very small fragments

Very small RBC, RBC fragments and WBC fragments may be the cause in increased PLT count.

Agglutinated RBC

PLT may be trapped in the agglutinated RBC resulting in decrease in PLT. This can be checked by abnormal

MCH and MCHC values and examination of the stained blood film.

Very large PLT

Large PLT may exceed the PLT threshold and might not be counted which results in low PLT count.

Chemotherapy

Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT count.

In such a case, measure manually.

Hemolysis

Hemolyzed specimens contain red cell stroma which may increase PLT count.

Anticoagulated blood

Blood anticoagulated with acid-citrate-dextrose may have clumped PLT which may cause decrease in PLT

count.

Agglutinated PLT

Clumped PLT may decrease PLT count and/or increase WBC count. For such sample, collect the sample in

sodium citrate anticoagulant and measure only PLT. The PLT result must be corrected for the sodium citrate

dilution effect.

MPV: Very large PLT

Large PLT may exceed the PLT threshold and not be counted which results in low MPV.

Very small fragments

Very small RBC, RBC fragments and WBC fragments may interfere with MPV measurement.

Agglutinated RBC

PLT may be trapped in the agglutinated RBC resulting in erroneous MPV. This can be checked by abnormal

MCH and MCHC values and examination of the stained blood film.

1.14 Service Manual MEK-6400/6410/6420

1. GENERAL

Chemotherapy

Cytotoxic and immunosuppressive drugs may affect MPV. In such a case, measure manually.

NOTE

Blood samples collected in EDTA do not maintain stable MPV because platelets swell

depending on the interval after collection and storage temperature.

WBC differential parameters are derived from the WBC count, therefore, the limitations for WBC also affect these

parameters.

LY and LY%: Erythroblasts, certain parasites and RBC that are resistant to lysis may interfere with an accurate LY

count.

MO and MO%: Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils may interfere with

an accurate MO count.

GR and GR%: Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may

interfere with an accurate GR count and GR%.

EO: Abnormal granules may interfere with an accurate EO count.

Service Manual MEK-6400/6410/6420 1.15

Section 2 Troubleshooting

Check Procedure Flowchart ............................................................................................................................... 2.4

Operating Condition and Blood Sample Checks ................................................................................................. 2.5

Operating Condition Check ...................................................................................................................... 2.5

Blood Sample Handling Check ................................................................................................................. 2.5

Pre-dilution Sample Preparation Check .................................................................................................... 2.5

Checking the Hematology Analyzer ................................................................................................................... 2.7

Checking Background Noise .................................................................................................................... 2.7

Measurement ................................................................................................................................. 2.7

Parameter Data Check with Diluent ................................................................................................ 2.7

Reducing Background Noise .......................................................................................................... 2.8

Background Noise Problems .......................................................................................................... 2.9

Checking the Reproducibility .................................................................................................................... 2.9

Checking Procedure ....................................................................................................................... 2.9

CV Values ..................................................................................................................................... 2.10

Checking Accuracy ...........................................................................................................................................2.11

Checking Procedure ................................................................................................................................ 2.11

Poor WBC Reproducibility ....................................................................................................................... 2.11

Poor HGB Reproducibility........................................................................................................................2.12

Poor RBC Reproducibility ........................................................................................................................2.13

Poor PLT Reproducibility ......................................................................................................................... 2.14

Poor HCT or MCV Reproducibility ........................................................................................................... 2.15

Solving Problems from Alarm Messages .......................................................................................................... 2.16

A001: No diluent ........................................................................................................................... 2.16

A005: No detergent ....................................................................................................................... 2.16

A007: No lysing reagent ................................................................................................................ 2.17

A009: WBC priming error ............................................................................................................... 2.17

A010: RBC PRIMING ERROR ......................................................................................................2.17

A021: WBC level 1 ........................................................................................................................2.17

A022: WBC level 2 ........................................................................................................................2.18

A023: WBC level 3 ........................................................................................................................2.18

A024: WBC bubble 1 ..................................................................................................................... 2.18

A025: WBC bubble 2 ..................................................................................................................... 2.18

A026: WBC bubble 3 ..................................................................................................................... 2.19

A027: WBC bubble 4 ..................................................................................................................... 2.19

A029: WBC clog ............................................................................................................................2.19

A030: WBC sample error ...............................................................................................................2.19

A031: WBC noise 2 .......................................................................................................................2.20

A032: WBC noise 1 .......................................................................................................................2.20

A036: WBC upper manometer dirty ............................................................................................... 2.20

A037: WBC lower manometer dirty ................................................................................................ 2.20

A041: RBC level 1 ........................................................................................................................ 2.21

A042: RBC level 2 ........................................................................................................................ 2.21

A043: RBC level 3 ........................................................................................................................ 2.21

A044: RBC bubble 1 ...................................................................................................................... 2.22

A045: RBC bubble 2 ...................................................................................................................... 2.22

A046: RBC bubble 3 ...................................................................................................................... 2.22

Service Manual MEK-6400/6410/6420 2.1

A047: RBC bubble 4 ...................................................................................................................... 2.22

A049: RBC clog ............................................................................................................................ 2.22

A050: RBC sample error ............................................................................................................... 2.23

A051: RBC noise 2 ....................................................................................................................... 2.23

A052: PLT noise 1 ........................................................................................................................ 2.23

A053: PLT noise 3 ........................................................................................................................ 2.23

A057: RBC upper manometer dirty ................................................................................................ 2.23

A058: RBC lower manometer dirty ................................................................................................2.23

A061: HGB voltage low ................................................................................................................. 2.24

A062: HGB voltage high ................................................................................................................ 2.24

A063: HGB circuit error ................................................................................................................. 2.24

A072: Tube holder open .................................................................................................................2.24

A073: Tube in the holder ................................................................................................................2.25

A091: Room temperature high .......................................................................................................2.25

A092: Room temperature low ........................................................................................................ 2.25

A093: Internal temperature high .................................................................................................... 2.25

A094: Internal temperature low ......................................................................................................2.25

A095: Power supply temp high ...................................................................................................... 2.25

! APPEARS ON THE RIGHT OF WBC MEASURED VALUE ........................................................ 2.26

! APPEARS ON THE RIGHT OF MCHC MEASURED VALUE ...................................................... 2.26

C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or PLT Clumps appears .... 2.27

OTHER FLAGS............................................................................................................................. 2.27

Solving Problems from System Error Messages ...............................................................................................2.28

E001: DILUTER INITIALIZE ERROR ............................................................................................ 2.28

E021: SAMPLER INITIALIZE ERROR .......................................................................................... 2.28

E041: SUB BATH INITIALIZE ERROR .......................................................................................... 2.28

E101: BATH DRAIN ERROR ......................................................................................................... 2.29

E122: CHECK SETTINGS ............................................................................................................ 2.29

E123: MEMORY ERROR .............................................................................................................. 2.29

E124: CIRCUIT ERROR ................................................................................................................2.29

E126: TOUCH PANEL ERROR ...................................................................................................... 2.29

E141: CAP PIERCE INITIALIZE ERROR ..................................................................................... 2.29

Service Maintenance Screens...........................................................................................................................2.30

Displaying the SERVICE Screen ............................................................................................................. 2.30

CHECK MENU Screen ............................................................................................................................ 2.32

UNIT MAINTE Screen ............................................................................................................................ 2.32

MD-640V ...................................................................................................................................... 2.33

MS-640V ...................................................................................................................................... 2.33

MS-641V (MEK-6400 only) ........................................................................................................... 2.34

MV & PUMP CHK ........................................................................................................................ 2.34

CONT MEASURE Screen .......................................................................................................................2.35

SETTING MENU Screen ........................................................................................................................ 2.35

ADV. SETTINGS Screen ...............................................................................................................2.35

REAGENT FACTOR Screen ......................................................................................................... 2.36

USER MAINTENANCE Screen ..................................................................................................... 2.36

PANIC VALUE Screen (for MEK-6400 and MEK-6410) .................................................................. 2.37

FLAG SETTINGS Screen ............................................................................................................. 2.37

BAR CODE PORT Screen ............................................................................................................2.37

UNIT CAL Screen ......................................................................................................................... 2.38

2.2 Service Manual MEK-6400/6410/6420

MONITOR Screen ................................................................................................................................... 2.38

X10-CV screen ....................................................................................................................................... 2.39

INITIAL MENU Screen ............................................................................................................................ 2.40

OTHER MENU Screen ............................................................................................................................2.40

PRINT SETTINGS Screen ...................................................................................................................... 2.40

Service Manual MEK-6400/6410/6420 2.3

2. TROUBLESHOOTING

Check Procedure Flowchart

Check the hematology analyzer according to the following check procedure flowchart.

Are the following conditions acceptable?

• Reagents

• Temperature

• Blood sample

Ye s

Does the screen appear with no error messages, such

as the following, when the power is turned on?

• No diluent

• No detergent

• No lysing reagent

• xxx ... initialize error

• xxx priming error

Refer to the “Operating Condition and Blood Sample

Checks” section.

Refer to the “Solving Problems from Alarm Messages”

and “Solving Problems from System Error Messages”

sections.

Ye s

Check the specified parameters after the background

noise measurement.

• Are the WBC, RBC, HGB and PLT data

acceptable?

• Is there no alarm message?

Ye s

Check the following after the MEK-3D hematology

control measurement.

• Is there no alarm message?

• Are the following parameter reproducibility

acceptable?

RBC, HGB, WBC, PLT, HCT and MCV

• Are all the obtained parameters within the

acceptable range on the assay sheet?

Ye s

Refer to the “Checking Background Noise” section,

“Background Noise Problems” table and “Solving

Problems from Alarm Messages” section.

Refer to the “Solving Problems from Alarm Messages”

and “Checking Accuracy” sections.

END

2.4 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

Operating Condition and Blood Sample Checks

Operating Condition Check

Measurement requires the following operating conditions.

• Operating temperature: 15 to 30°C (59 to 86°F)

• Operating humidity: 30 to 85%

• Operating atmospheric pressure:70 to 106 kPa

If the diluent or hemolysing reagent temperature is lower than 15°C, it will

affect the measurement data of HGB, WBC and WBC differential parameters or

cause a hemolysing error or sample error alarm.

Blood Sample Handling Check

• Check that the blood sample is counted immediately after collection or

stored in a cool place (at temperatures between 2 and 8°C, 36 and 46°F) such

as a refrigerator. If the blood sample is stored in a refrigerator at temperatures

lower than 2°C for a long time or left more than 12 hours at room temperature

after collection, the WBC differential parameters may be affected.

If the blood sample causes hemolysing error when it is counted

within 30 minutes after collection, wait at least 30 minutes after

collection before counting it.

• Measurement data might not be accurate for special blood sample, such as

from a neonate or patient with hepatic disease or special treatment. In most

cases, these blood samples are difficult to hemolyze because RBC have

strong membrane resistance and the analyzer counts high WBC. Bilirubin or

WBC in blood affects MCHC. These special blood samples need a different

measurement method.

Pre-dilution Sample Preparation Check

Most measurement data errors in pre-dilution mode are caused by blood

clotting and dilution of the blood sample. Therefore, check the following

points. When pre-dilution blood is measured, data accuracy depends on careful

clotting and diluting.

NOTE

• Before using a sahli pipette for pre-dilution blood collection, check that the

sahli pipette is completely cleaned and dried. An optional microcap (20 µL)

is recommended for pre-dilution blood collection. (Supply code no. T812).

• When putting the pre-dilution blood into a sample cup, make sure there are

no bubbles in the cup. If you cannot avoid making bubbles with the sahli

pipette, we recommend the optional microcap which prevents bubbling.

Service Manual MEK-6400/6410/6420 2.5

2. TROUBLESHOOTING

• In pre-dilution mode, about 1 mL of sample is aspirated. Make sure that the

sampling nozzle is near the bottom of the sample cup so that the correct

volume of the sample can be aspirated.

2.6 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

Checking the Hematology Analyzer

There are two hematology analyzer checks: background noise and

reproducibility.

Checking Background Noise

Measure the diluent to check the background noise.

Measurement

Count the diluent in closed mode and open mode. Closed mode is only for

MEK-6400.

1. Select “Closed” or “Open” for <Sampling mode> and press the MENU key

on the READY screen.

2. Press the OTHER key on the MENU screen.

3. Press the BACKGROUND key on the OTHER screen. The “Measure

background noise?” message appears on the screen.

4. Press the YES key to measure background noise. The result is displayed

after the measurement is complete.

Parameter Data Check with Diluent

Check that the background check values are less than or equal to the following

values. Disregard the other parameter values because noise does not affect the

other parameters.

Especially check the data for the PLT parameter because PLT is more affected

by dust particles than the other parameters. When there are dust particles

smaller than the WBC and RBC parameters in the diluent, the WBC and RBC

are not affected by dust but the PLT value increases because the platelets are

about the same size as the dust particles. If PLT is over 10 × 103/µL, do the

action described below to reduce the background noise.

Best values Acceptable values Values in the operator’s

manual

WBC (× 103/µL): 0.0 0.1 0.2

RBC (× 106/µL): 0.00 0.01 0.05

HGB (g/dL): 0.0 0.1 0.1

PLT (× 103/µL): 3 9 10

Service Manual MEK-6400/6410/6420 2.7

2. TROUBLESHOOTING

Reducing Background Noise

To reduce the background noise when the background check value is outside

the optimal range shown in the previous tables, do the following.

1. Press the [Clean] key on the front panel to perform cleaning. If this does not

reduce the background noise, do the following steps.

2. Thoroughly clean the diluent container with detergent.

3. Wash away any remaining detergent in the hydraulic system with clean

diluent.

4. Perform the background check to make sure that the background noise is

reduced.

If the data of the background check is still not optimum, replace the diluent

with diluent from a new, sealed container.

NOTE

• Once the background noise is reduced and when the instrument is

used every day, the instrument is not severely contaminated.

However, contamination builds up in the instrument and cannot be

easily removed if the instrument is not cleaned periodically.

Periodic maintenance is important for keeping the instrument in

optimum condition.

• When not using the instrument for a long time, clean the fluid path

with diluent. Refer to “Storing and Transporting the Analyzer” in

Section 9 of the Operator’s Manual.

2.8 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

Background Noise Problems

Problem Possible Cause/Criteria Countermeasure

High background noise for all parameters.

High background noise of WBC, RBC and PLT.

High background noise of HGB.

The diluent or diluent container is dirty.

The fluid path inside the hematology analyzer is dirty.

Noise interference through AC source or other instruments nearby.

The reagents are not used in the appropriate temperature range and they are deteriorated. The filters are clogged or dirty. Clean or replace the filters with new ones. The measurement baths and sub baths are dirty. The apertures are dirty. Clean or replace the apertures with new ones. The rinse unit is dirty. Clean the rinse unit. The apertures are damaged. Replace the apertures with new one. The MC-640V measuring unit is damaged. Replace the unit with a new one. The sampling nozzle is clogged. Replace the sampling nozzle with a new one. The MD-640V combination syringe pump unit failure. The UT-7192 AMP CONTROL board failure. The UT-7193 POWER board failure. Replace the board with a new one. The reagent is dirty. Replace the reagent with a new one. The measurement baths and sub baths are dirty. The MC-640V measuring unit failure. Replace the unit with a new one. The UT-7201 HGB AMP board failure. Replace the board with a new one. The UT-7202 HGB LED board failure. Replace the board with a new one. The UT-7192 AMP CONTROL board failure. The UT-7193 POWER board failure. Replace the board with a new one. The reagent is dirty. Replace the reagent with a new one.

Clean the diluent container or replace the diluent with new one. Press the [Clean] key on the front panel to perform cleaning. Perform strong cleaning. Connect the hematology analyzer power cord to another AC outlet. If possible, use an independent AC outlet only for the hematology analyzer. Perform equipotential grounding and remove the other instruments if they are the cause of noise. Use the reagents in the appropriate temperature range.

Clean or replace the measurement baths and sub baths with new ones.

Replace the unit with a new one.

Replace the board with a new one.

Clean or replace the measurement baths and sub baths with new ones.

Replace the board with a new one.

Checking the Reproducibility

This check is used to check reproducibility of the instrument, using printout of

the CV values of 10 data of a diluted sample of the same MEK-3DN

hematology control. When the CV values are out of the specification range, the

reproducibility of the instrument is poor. If the reproducibility is found to be

poor, this printed result is used to troubleshoot the instrument.

Checking Procedure

1. Reduce the background noise.

2. Warm the sample (MEK-3DN) to room temperature (15 to 30°C, 59 to 86°F)

and stir it sufficiently.

3. Measure the sample 10 times.

Service Manual MEK-6400/6410/6420 2.9

2. TROUBLESHOOTING

4. Press the MENU → OTHER → SERVICE → X10-CV key to display the

result and check the CV data. (The type of user must be “Service” to enter

the SERVICE screen.)

CV Values

The reproducibility is checked using the CV values. The CV value is acquired

by dividing the standard deviation by the average value. When the CV value is

large, it indicates that the measurement data variability is large (i.e.

reproducibility is poor).

Example

Parameters

WBC LY% 5.0

MO% 12.0

GR% 5.0

RBC

HGB 13.0 (g/dL) 0.7 1.5

MCV 91 (fL) 0.4 1.0

PLT

MPV 1.0

Measured Value

(average)

6.8 (×103/µL)

4.43 (×106/µL)

242 (×103/µL)

Acquired CV

(%)

1.3 2.0

0.8 1.5

3.2 4.0

Standard CV

(%)

In the above example, the RBC measured value is 4.43 and the acquired CV is

0.8%. 0.8% of 4.43 is 0.035, 4.43 ± 0.035 is 4.465 and 4.395. Therefore, the CV

of 0.8% indicates that in 6 of 10 measurements, the acquired value is between

4.395 and 4.465.

4.37 4.39 4.40 4.43 4.44 4.45 4.45 4.46 4.47 4.48

(2 data) (6 data) (2 data)

4.395 4.465

Reference

The reproducibility depends on the number of counted cells. When this number

increases, the reproducibility is improved. Therefore, if the number of counted

cells is small (a sample with low concentration is measured), the variation

increases. For example, the CV is within 4% when counting 300,000 PLT, but

the CV increases to over 4% when counting 100,000 PLT. This is a common

feature for any hematology analyzer.

2.10 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

Checking Accuracy

Checking Procedure

Before checking accuracy, measure a MEK-3DN hematology control with the

procedure described in the “Checking the Reproducibility” section. Confirm

that the obtained sample data is within the acceptable range on the assay sheet

attached to the hematology control. If the data is outside this range or on the

borderline, calibrate the hematology analyzer with the following procedure.

1. Measure the MEK-3DN hematology control 10 times on the

CALIBRATION screen.

2. Calibrate the instrument. Refer to Section 7 “Calibration” in the Operator’s

Manual.

3. Remeasure the hematology control. Confirm that the obtained sample data

is within the acceptable range on the assay sheet attached to the

hematology control.

Poor WBC Reproducibility

The following may be the main reasons for poor WBC reproducibility.

• Measuring unit and fluid path are dirty

• Reagent is deteriorated.

• Samples are not stirred enough.

• Hematology analyzer failure.

Measurement baths, rinse unit or fluid path is dirty

Possible Cause/Criteria Countermeasure

WBC measurement bath is dirty.

Rinse unit is dirty. Clean the rinse unit. WBC aperture is dirty. Clean or replace the WBC aperture with a new one.

Deterioration of reagents

Possible Cause/Criteria Countermeasure

The specified reagents are not used. Use only the specified reagents. Deterioration of reagents.

The reagents are used outside the specified temperature range. Use the reagents in the appropriate temperature.

Press the [Clean] key on the front panel to perform cleaning. Perform strong cleaning. Clean the WBC measurement bath. Replace the WBC sub bath with a new one. Replace the WBC measurement bath with a new one. Replace the hemoglobin filter below air trap with a new one.Drain path of WBC measurement bath is dirty. Clean the air trap with a cotton swab.

Replace the reagents with new one. Use the reagents in appropriate conditions.

Service Manual MEK-6400/6410/6420 2.11

2. TROUBLESHOOTING

Samples are not stirred enough

Possible Cause/Criteria Countermeasure

The sample was not stirred thoroughly before measurement. Stir the sample thoroughly before measurement.

Hematology analyzer failure

Possible Cause/Criteria Countermeasure

The appropriate amount of hemolyzing reagent is not dispensed.

Circuit malfunction (WBC is outside 8.0 ±5% when circuit check is performed by pressing MENU key OTHER key → CIRCUIT CHECK key)

WBC sample error

→

Put the HEMOLYNAC3 tube assay into the middle of the bottle. Replace the HEMOLYNAC3 tube assay with a new one. Replace the MD-640V combination syringe pump unit with a new one.

Replace the MC-640V measuring unit with a new one.

Replace the UT-7198 MEASURING board with a new one.

Replace the UT-7192 AMP CONTROL board with a new one.

Replace the MD-640V combination syringe pump unit with a new one. In closed mode, replace the MS-641V cap pierce unit with a new one. In open mode, replace the MS-640V sampler unit with a new one.

Poor HGB Reproducibility

The following may be the main reasons for poor HGB reproducibility.

• Measuring unit and fluid path are dirty.

• Reagent is deteriorated.

• Samples are not stirred enough.

• Hematology analyzer failure.

Measurement bath, rinse unit or fluid path is dirty

Possible Cause/Criteria Countermeasure

WBC measurement bath is dirty.

dirty. Rinse unit is dirty. Clean the rinse unit.

Deterioration of reagents

Possible Cause/Criteria Countermeasure

The specified reagents are not used. Use only the specified reagents. Deterioration of reagents.

The reagents are used outside the specified temperature range. Use the reagents in the appropriate temperature.

Press the [Clean] key on the front panel to perform cleaning. Perform strong cleaning. Clean the WBC measurement bath. Replace the WBC sub bath with a new one. Replace the WBC measurement bath with a new one. Replace the hemoglobin filter below air trap with a new one.Drain path of the WBC measurement bath is Clean the air trap with a cotton swab.

Replace the reagents with new one. Use the reagents in appropriate conditions.

Samples are not stirred enough

Possible Cause/Criteria Countermeasure

The sample was not stirred thoroughly before measurement. Stir the sample thoroughly before measurement.

2.12 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

Hematology analyzer failure

Possible Cause/Criteria Countermeasure

The appropriate amount of hemolyzing reagent is not dispensed.

HGB sample error

HGB sensor output failure (HGB ON item is outside 1.5 to 4.5 V range when sensor check is performed by pressing MENU key → OTHER key SENSOR MONITOR key)

Poor RBC Reproducibility

Put the HEMOLYNAC3 tube assay into the middle of the bottle. Replace the HEMOLYNAC3 tube assay with a new one. Replace the MD-640V combination syringe pump unit with a new one. Replace the MD-640V combination syringe pump unit with a new one. In closed mode, replace the MS-641V cap pierce unit with a new one. In open mode, replace the MS-640V sampler unit with a new one. Adjust the HGB variable resistance volume so that HGB ON item is 3.0 V

1 V. Refer to “Adjusting the HGB Sensor Output Voltage” in Section 5.

Replace the MC-640V measuring unit with a new one.

→

Replace the UT-7201 HGB AMP board with a new one. Replace the UT-7202 HGB LED board with a new one. Replace the UT-7192 AMP CONTROL board with a new one.

The following may be the main reasons for poor RBC reproducibility.

• Measuring unit and fluid path are dirty.

• Samples are not stirred enough.

• Hematology analyzer failure.

Measurement bath, rinse unit or fluid path is dirty

Possible Cause/Criteria Countermeasure

RBC measurement bath is dirty.

Drain path of the RBC measurement bath is dirty. Rinse unit is dirty. Clean the rinse unit. RBC aperture is dirty. Clean or replace the RBC aperture with a new one.

Samples are not stirred enough

Possible Cause/Criteria Countermeasure

The sample was not stirred thoroughly before measurement. Stir the sample thoroughly before measurement.

Hematology analyzer failure

Possible Cause/Criteria Countermeasure

Circuit malfunction (RBC is outside 1.60 ±5% when circuit check is performed by pressing MENU key

OTHER key → CIRCUIT CHECK key)

→

RBC sample error

Press the [Clean] key on the front panel to perform cleaning. Perform strong cleaning. Clean the RBC measurement bath. Replace the RBC sub bath with a new one. Replace the RBC measurement bath with a new one. Clean or replace the filter below RBC measurement bath with a new one.

Replace the MC-640V measuring unit with a new one.

Replace the UT-7198 MEASURING board with a new one.

Replace the UT-7192 AMP CONTROL board with a new one.

Replace the MD-640V combination syringe pump unit with a new one. In closed mode, replace the MS-641V cap pierce unit with a new one. In open mode, replace the MS-640V sampler unit with a new one.

Service Manual MEK-6400/6410/6420 2.13

2. TROUBLESHOOTING

Poor PLT Reproducibility

The following may be the main reasons for poor PLT reproducibility.

• Background noise is high. PLT measurement is highly sensitive. Therefore,

the background noise must be within the acceptable range (PLT < 10 × 103/

µL). Before checking the PLT reproducibility, check the background noise.

• Samples are not stirred enough.

• Measurement settings are inappropriate.

• Dilution error.

• Hematology analyzer failure.

Background noise is high

Possible Cause/Criteria Countermeasure

The diluent is dirty. Replace the diluent with a new one. The diluent bottle is dirty. Clean the diluent bottle. The fluid path is dirty.

Rinse unit is dirty. Clean the rinse unit. The sampling nozzle is clogged. Replace the sampling nozzle with a new one.

The aperture is damaged. Replace the aperture with a new one. The MC-640V measuring unit failure Replace the unit with a new one. Circuit malfunction (PLT is outside 160 ±5% when circuit check is performed by pressing MENU key OTHER key → CIRCUIT CHECK key)

→

Press the [Clean] key on the front panel to perform cleaning. Perform strong cleaning. Clean the sub baths and measurement baths. Clean or replace the filter below the RBC measurement bath with a new one. Clean the apertures. Replace the measurement baths and sub baths with new ones. Replace the aperture with a new one.

Connect the power cord to another AC outlet.Noise through AC source Put a noise cut filter on the power cord. Perform equipotential grounding.Noise interference Move the instrument which is producing the noise away from the hematology analyzer.

Replace the UT-7198 MEASURING board with a new one.

Replace the MC-640V measuring unit with a new one.

Replace the UT-7192 AMP CONTROL board with a new one.

Samples are not stirred enough

Possible Cause/Criteria Countermeasure

The sample was not stirred thoroughly before measurement. Stir the sample thoroughly before measurement.

Inappropriate measurement settings

Possible Cause/Criteria Countermeasure

The sensitivity and threshold settings are not appropriate.

Dilution error

Possible Cause/Criteria Countermeasure

The MD-640V combination syringe pump unit failure.

2.14 Service Manual MEK-6400/6410/6420

Check that the sensitivity and threshold settings are set as below. RBC SENSITIVITY: 5 RBC THRESHOLD: AUTO PLT THRESHOLD: 5

Replace the sampling nozzle with a new one.The MS-640V sampler unit failure. Replace the unit with a new one. Replace the unit with a new one.

2. TROUBLESHOOTING

Hematology analyzer failure

Possible Cause/Criteria Countermeasure

The aperture is dirty. Clean the aperture. The aperture is damaged. Replace the aperture with a new one. Circuit malfunction (PLT is outside 160 ±5% when circuit check is performed by pressing MENU key OTHER key → CIRCUIT CHECK key)

The MC-640V measuring unit is not attached properly. The MC-640V measuring unit failure. Replace the unit with a new one.

→

Replace the UT-7198 MEASURING board with a new one.

Replace the MC-640V measuring unit with a new one.

Replace the UT-7192 AMP CONTROL board with a new one.

Reattach the unit. Make sure to tighten the screws attaching the unit to the chassis. Refer to “Removing the Measuring Unit” in Section 4.

Poor HCT or MCV Reproducibility

The following may be the main reasons for poor HCT or MCV reproducibility.

• Measuring unit is dirty

• Measurement settings are inappropriate.

• Hematology analyzer failure.

Measuring unit is dirty

Possible Cause/Criteria Countermeasure

Measurement baths and sub baths are dirty. Clean the measurement baths and sub baths. Aperture is dirty. Clean the aperture.

Inappropriate measurement settings

Possible Cause/Criteria Countermeasure

The sensitivity and threshold settings are not appropriate.

Hematology analyzer failure

Possible Cause/Criteria Countermeasure

The aperture is damaged. Replace the aperture with a new one. Circuit malfunction (RBC is outside 1.60 ±5% when circuit check is performed by pressing MENU key

OTHER key → CIRCUIT CHECK key)

→

RBC sample error

Check that the sensitivity and threshold settings are set as below. RBC SENSITIVITY: 5 RBC THRESHOLD: AUTO

Replace the MC-640V measuring unit with a new one.

Replace the UT-7198 MEASURING board with a new one.

Replace the UT-7192 AMP CONTROL board with a new one.

Replace the MD-640V combination syringe pump unit with a new one. In closed mode, replace the MS-641V cap pierce unit with a new one. In open mode, replace the MS-640V sampler unit with a new one.

Service Manual MEK-6400/6410/6420 2.15

2. TROUBLESHOOTING

Solving Problems from Alarm Messages

A001: No diluent

This message appears when the sensor in the tank does not detect the diluent during measurement, priming or cleaning.

Reason Possible Cause/Criteria Countermeasure

No diluent in the tank

Air bubbles

Failure of the sensor in the tank

Wrong tube The cleanac tube 8 (tube for CLEANAC3)

Out of diluent. Replace diluent. The tube between the hematology analyzer and diluent is squeezed, bent, clogged or there is leakage. Leakage in the fluid path. Check the fluid path. If necessary, replace tubes. Pump tube is damaged. Replace the pump tube with a new one. The MP-640V pump unit failure. Replace the unit with a new one. The MD-640V combination syringe pump unit failure. The UT-7192 AMP CONTROL board failure. The UT-7193 POWER board failure. Replace the board with a new one. When the hematology analyzer is left unused, the diluent inside the tank may evaporate and enter the fluid path as air bubbles. Leakage in the fluid path. Check the fluid path. If necessary, replace tubes. Air bubbles on the liquid sensor. Perform priming. Voltage adjustment error of the liquid sensor. Liquid sensor failure. Replace the UT-7199 LIQUID SENSOR board with

is used for diluent.

Check the tube connection and if necessary, replace the tube with a new one.

Replace the unit with a new one.

Replace the board with a new one.

Press the [Clean] key to perform cleaning, press the [Reset] and [Power] keys together to turn the power off, then press the [Power] key to turn on.

Adjust the voltage. Refer to “Adjusting the Liquid Sensor Output Voltages” in Section 5.

a new one. Use the diluent tube.

A005: No detergent

This message appears when the liquid sensors on the JQ-640V inlet/outlet unit do not detect the detergent during

priming or cleaning.

Reason Possible Cause/Criteria Countermeasure

No detergent flow

Failure of the sensor in the tank

The tube between the hematology analyzer and CLEANAC detergent is squeezed, bent, clogged or there is leakage. Pump tube is damaged. Replace the pump tube with a new one. The MC-640V measuring unit failure. Replace the unit with a new one. The MP-640V pump unit failure. Replace the unit with a new one. Air bubbles on the liquid sensor. Perform priming. Voltage adjustment error of the liquid sensor. Liquid sensor failure. Replace the UT-7199 LIQUID SENSOR board with

Check the tube connection and if necessary, replace the tube with a new one.

Adjust the voltage. Refer to “Adjusting the Liquid Sensor Output Voltages” in Section 5.

a new one.

2.16 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

A007: No lysing reagent

This message appears when the sensors on the JQ-640V inlet/outlet unit do not detect the hemolysing reagent during

measurement, priming or cleaning.

Reason Possible Cause/Criteria Countermeasure

No hemolyzing reagent flow

Failure of the sensor

A009: WBC priming error

This message appears when diluent cannot be primed during priming the WBC manometer (manometer upper or lower

sensor does not detect the diluent).

Reason Possible Cause/Criteria Countermeasure

Liquid cannot be detected even when there is enough liquid (LED does not light)

manometer

The tube between the hematology analyzer and HEMOLYNAC3 hemolysing reagent is squeezed, bent, clogged or there is leakage. The MD-640V combination syringe pump unit failure. Air bubbles on the liquid sensor. Perform priming. Voltage adjustment error of the liquid sensor. Adjust the voltage. Refer to “Adjusting the Liquid

Liquid sensor failure. Replace the UT-7199 LIQUID SENSOR Board with

WBC manometer upper sensor adjustment error.

WBC manometer lower sensor adjustment error.

The MC-640V measuring unit failure. Replace the unit with a new one. WBC aperture is not attached properly. Reattach the WBC aperture.Air bubbles in the The MC-640V measuring unit failure. Replace the unit with a new one.

Check the tube connection and if necessary, replace the tube with a new one.

Replace the unit with a new one.

Sensor Output Voltages” in Section 5.

a new one.

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

A010: RBC PRIMING ERROR

This message appears when diluent cannot be primed during priming the RBC manometer (manometer upper or lower

sensor does not detect the diluent).

Reason Possible Cause/Criteria Countermeasure

Liquid cannot be detected even when there is enough liquid (LED does not light)

manometer

A021: WBC level 1

This message appears when the manometer upper sensor does not detect no liquid at the start of WBC measurement or

draining liquid from the manometer.

Reason Possible Cause/Criteria Countermeasure

Liquid is drained but liquid cannot be detected (LED does not turn off)

Liquid cannot be drained

RBC manometer upper sensor adjustment error.

RBC manometer lower sensor adjustment error.

The MC-640V measuring unit failure. Replace the unit with a new one. RBC aperture is not attached properly. Reattach the RBC aperture.Air bubbles in the The MC-640V measuring unit failure. Replace the unit with a new one.

WBC manometer is dirty. Perform strong cleaning. WBC manometer upper sensor adjustment error.

The MC-640V measuring unit failure. Replace the unit with a new one. Pump tube is damaged. Replace the pump tube with a new one. The MP-640V pump unit failure. Replace the unit with a new one. The UT-7193 POWER board failure. Replace the board with a new one. The MC-640V measuring unit failure. Replace the unit with a new one. Electromagnetic valve failure.

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

Replace the UT-7192 AMP CONTROL board with a new one. Replace the UT-7193 POWER board with a new one.

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

Service Manual MEK-6400/6410/6420 2.17

2. TROUBLESHOOTING

A022: WBC level 2

This message appears when the manometer upper sensor detects no liquid but the lower sensor does not detect no liquid

at the start of WBC measurement or draining liquid from the manometer.

Reason Possible Cause/Criteria Countermeasure

Liquid is drained but liquid cannot be detected (LED does not turn off)

Liquid cannot be drained

WBC manometer is dirty. Perform strong cleaning. WBC manometer lower sensor adjustment error.

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

Replace the UT-7192 AMP CONTROL board with a new one. Replace the UT-7193 POWER board with a new one.

A023: WBC level 3

This message appears when the manometer lower sensor does not detect the liquid during WBC measurement or

aspirating sample.

Reason Possible Cause/Criteria Countermeasure

The pump tube is damaged. Replace the pump tube with a new one.Cannot produce measuring

pressure

produced but there is leakage

produced but there is clog

The MC-640V measuring unit failure. Replace the unit with a new one. WBC aperture is not attached properly. Reattach the WBC aperture properly.Measuring pressure can be The MC-640V measuring unit failure. Replace the unit with a new one. WBC aperture is clogged. Clean or replace the aperture with a new one.Measuring pressure can be The MC-640V measuring unit failure. Replace the unit with a new one.

A024: WBC bubble 1

This message appears when the manometer upper or lower sensor detects no liquid during WBC measurement or at the

start of draining manometer.

Reason Possible Cause/Criteria Countermeasure

Diluent cannot be detected even when there is enough diluent (LED does not light)

WBC manometer is dirty. Perform Strong Cleaning. WBC manometer upper sensor adjustment error. WBC manometer lower sensor

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in

Section 5. adjustment error. The MC-640V measuring unit failure. Replace the unit with a new one.

Air bubbles in the manometer

WBC manometer is dirty. Perform strong cleaning. WBC aperture is not attached properly. Reattach the WBC aperture with a new one. The MC-640V measuring unit failure. Replace the unit with a new one.

A025: WBC bubble 2

This message appears when the manometer upper sensor detects the liquid during WBC measurement or draining

manometer (from upper sensor OFF to lower sensor OFF).

Reason Possible Cause/Criteria Countermeasure

WBC manometer is dirty. Perform strong cleaning.Air bubbles in the

manometer

The MC-640V measuring unit failure. Replace the unit with a new one.

2.18 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

A026: WBC bubble 3

This message appears when the manometer lower sensor detects no liquid during WBC measurement or aspirating sample

(from lower sensor ON to upper sensor ON).

Reason Possible Cause/Criteria Countermeasure

Air bubbles in the manometer

A027: WBC bubble 4

This message appears when the WBC measurement or aspirating sample time is too short (from lower sensor ON to upper

sensor ON).

Reason Possible Cause/Criteria Countermeasure

large.

manometer

A029: WBC clog

This message appears when the manometer lower sensor detects the liquid but the upper sensor does not detect the liquid

during WBC measurement or aspirating sample.

WBC manometer is dirty. Perform strong cleaning. WBC aperture is not attached properly. Reattach the WBC aperture. The MC-640V measuring unit failure. Replace the unit with a new one.

WBC aperture is damaged. Replace the WBC aperture with a new one.The aperture is too Incorrect aperture (100 µm). Use an appropriate aperture (80 µm). No liquid. Perform priming.Air bubbles in the The MC-640V measuring unit failure. Replace the unit with a new one.

Reason Possible Cause/Criteria Countermeasure

Measuring pressure can be produced but there is clog

measuring pressure Measuring pressure can be produced but there is leakage Measuring at high altitude (higher than

1,000 m above sea level)

A030: WBC sample error

This message appears when the voltage between the electrodes is out of acceptable range.

Reason Possible Cause/Criteria Countermeasure

Diluted sample error for WBC count

WBC aperture is clogged. Clean or replace the WBC aperture with a new one.

The MC-640V measuring unit failure. Replace the unit with a new one.

The pump tube is damaged. Replace the pump tube with a new one.Cannot produce The MC-640V measuring unit failure. Replace the unit with a new one.

WBC aperture is not attached properly. Reattach the WBC aperture properly.

The MC-640V measuring unit failure. Replace the unit with a new one.

High altitude mode needs to be set to On.

The diluent temperature is too low. Specified reagents are not used. Only use the specified reagents.

Set “High altitude” on ADVANCED SET/SETTINGS MENU of the SERVICE screen must be set to On.

The environment temperature must be from 15 to 30°C.

Service Manual MEK-6400/6410/6420 2.19

2. TROUBLESHOOTING

A031: WBC noise 2

This message appears when there is baseline wandering during measurement.

A032: WBC noise 1

This message appears when the time sequence of counted pulse is not stable.

Reason Possible Cause/Criteria Countermeasure

Power line

Influence from other malfunction

Measuring unit failure

2 pin power cord is used. Use 3 pin power cord. Ground lead is not connected. Perform equipotential grounding. Decrease in the power voltage. Do not share the power source with any other instrument. Measurement baths and apertures are dirty. Clean or replace the measurement baths and apertures

with new ones. Perform strong cleaning.

High background noise. Refer to “Background Noise Problems” earlier in this

section.

Poor hemolyzation of the sample. Refer to “! Appears on the right on WBC measured

value” later in this section. Sample aspiration is not stable (clog alarm). WBC aperture is dirty. Perform strong cleaning.

WBC aperture is damaged. Replace the WBC aperture with a new one. The screw securing the measurement bath is loose. The MC-640V measuring unit and chassis are not insulated (there may be leakage). The MC-640V measuring unit failure. Replace the unit with a new one.

Refer to “A029: WBC clog”.

Clean the WBC aperture.

Tighten the screw.

Remove the cause.

A036: WBC upper manometer dirty

The voltage of the upper liquid sensor on the manometer is 2.25 to 2.75V.

Reason Possible Cause/Criteria Countermeasure

Manometer is dirty The WBC manometer is dirty. Perform strong cleaning. Sensor voltage adjustment error

WBC manometer upper sensor adjustment error.

Adjust the WBC manometer upper sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

A037: WBC lower manometer dirty

The voltage of the lower liquid sensor on the manometer is 2.25 to 2.75V.

Reason Possible Cause/Criteria Countermeasure

Manometer is dirty The WBC manometer is dirty. Perform strong cleaning. Sensor voltage adjustment error

WBC manometer lower sensor adjustment error.

Adjust the WBC manometer lower sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

2.20 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

A041: RBC level 1

This message appears when the manometer upper sensor does not detect no liquid at the start of RBC measurement or

draining liquid from the manometer.

Reason Possible Cause/Criteria Countermeasure

Liquid is drained but liquid cannot be detected (LED does not turn off)

Liquid cannot be drained

A042: RBC level 2

This message appears when the manometer upper sensor detects no liquid but the lower sensor does not detect no liquid

at the start of RBC measurement or draining liquid from the manometer.

Reason Possible Cause/Criteria Countermeasure

Liquid is drained but liquid cannot be detected (LED does not turn off)

Liquid cannot be drained

RBC manometer is dirty. Perform strong cleaning. RBC manometer upper sensor adjustment error.

RBC manometer is dirty. Perform strong cleaning. RBC manometer lower sensor adjustment error.

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

Replace the UT-7192 AMP CONTROL board with a new one. Replace the UT-7193 POWER board with a new one.

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

Replace the UT-7192 AMP CONTROL board with a new one. Replace the UT-7193 POWER board with a new one.

A043: RBC level 3

This message appears when the manometer lower sensor does not detect the liquid during RBC measurement or

aspirating sample.

Reason Possible Cause/Criteria Countermeasure

The pump tube is damaged. Replace the pump tube with a new one.Cannot produce measuring

pressure

produced but there is leakage

produced but there is clog

Service Manual MEK-6400/6410/6420 2.21

The MC-640V measuring unit failure. Replace the unit with a new one. RBC aperture is not attached properly. Reattach the RBC aperture properly.Measuring pressure can be The MC-640V measuring unit failure. Replace the unit with a new one. RBC aperture is clogged. Clean or replace the aperture with a new one.Measuring pressure can be The MC-640V measuring unit failure. Replace the unit with a new one.

2. TROUBLESHOOTING

A044: RBC bubble 1

This message appears when the manometer upper or lower sensor detects no liquid during RBC measurement or at the

start of draining manometer.

Reason Possible Cause/Criteria Countermeasure

Diluent cannot be detected even when there is enough diluent (LED does not light)

RBC manometer is dirty. Perform strong cleaning. RBC manometer upper sensor adjustment error. RBC manometer lower sensor adjustment

Adjust the sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in

Section 5. error. The MC-640V measuring unit failure. Replace the unit with a new one.

Air bubbles in the manometer

RBC manometer is dirty. Perform strong cleaning. RBC aperture is not attached properly. Reattach the RBC aperture with a new one. The MC-640V measuring unit failure. Replace the unit with a new one.

A045: RBC bubble 2

This message appears when the manometer upper sensor detects the liquid during RBC measurement or when draining

manometer (from upper sensor OFF to lower sensor OFF).

Reason Possible Cause/Criteria Countermeasure

RBC manometer is dirty. Perform strong cleaning.Air bubbles in the

manometer

The MC-640V measuring unit failure. Replace the unit with a new one.

A046: RBC bubble 3

This message appears when the manometer lower sensor detects no liquid during RBC measurement or aspirating sample

(from lower sensor ON to upper sensor ON).

Reason Possible Cause/Criteria Countermeasure

Air bubbles in the manometer

RBC manometer is dirty. Perform strong cleaning. RBC aperture is not attached properly. Reattach the RBC aperture. The MC-640V measuring unit failure. Replace the unit with a new one.

A047: RBC bubble 4

This message appears when the RBC measurement or aspirating sample time is too short (from lower sensor ON to upper

sensor ON).

Reason Possible Cause/Criteria Countermeasure

RBC aperture is damaged. Replace the RBC aperture with a new one.The aperture is too

large.

manometer

Incorrect aperture (100 µm). Use an appropriate aperture (80 µm). No liquid. Perform priming.Air bubbles in the The MC-640V measuring unit failure. Replace the unit with a new one.

A049: RBC clog

This message appears when the manometer lower sensor detects the liquid but the upper sensor does not detect the liquid

during RBC measurement or aspirating sample.

Reason Possible Cause/Criteria Countermeasure

Measuring pressure can be produced but there is clog

measuring pressure Measuring pressure can be produced but there is leakage. Measuring at high altitude (higher than

1,000 m above sea level)

RBC aperture is clogged. Clean or replace the RBC aperture with a new one.

The MC-640V measuring unit failure. Replace the unit with a new one.

The pump tube is damaged. Replace the pump tube with a new one.Cannot produce The MC-640V measuring unit failure. Replace the unit with a new one.

RBC aperture is not attached properly. Reattach the RBC aperture properly.

The MC-640V measuring unit failure. Replace the unit with a new one.

High altitude mode needs to be set to On.

Set “High altitude” on ADVANCED SET/SETTINGS MENU of the SERVICE screen must be set to On.

2.22 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

A050: RBC sample error

This message appears when the voltage between the electrodes is out of acceptable range.

Reason Possible Cause/Criteria Countermeasure

Diluted sample error for RBC count

A051: RBC noise 2

This message appears when time sequence of the counted pulses is not stable.

A052: PLT noise 1

This message appears when there is baseline wandering during measurement.

A053: PLT noise 3

This message appears when the pulses are smaller than the threshold of counted pulses.

Reason Possible Cause/Criteria Countermeasure

Power line

Influence from other malfunction

Measuring unit failure

The diluent temperature is too low. Specified reagents are not used. Only use the specified reagents.

High background noise. Refer to “Background Noise Problems” earlier in this

Filter below the RBC measurement bath is clogged. Sample aspiration is not stable (clog alarm). RBC aperture is dirty. Perform strong cleaning.

RBC aperture is damaged. Replace the RBC aperture with a new one. The screw securing the measurement bath is loose. The MC-640V measuring unit and chassis are not insulated (there may be leakage). The MC-640V measuring unit failure. Replace the unit with a new one.

The environment temperature must be from 15 to 30°C.

with new ones. Perform strong cleaning.

section. Clean or replace the filter with a new one.

Refer to “A049: RBC clog”.

Clean the RBC aperture.

Tighten the screw.

Remove the cause.

A057: RBC upper manometer dirty

The voltage of the upper liquid sensor on the manometer is 2.25 to 2.75V.

Reason Possible Cause/Criteria Countermeasure

Manometer is dirty The RBC manometer is dirty. Perform strong cleaning. Sensor voltage adjustment error

A058: RBC lower manometer dirty

The voltage of the lower liquid sensor on the manometer is 2.25 to 2.75V.

Reason Possible Cause/Criteria Countermeasure

Manometer is dirty The RBC manometer is dirty. Perform strong cleaning. Sensor voltage adjustment error

Service Manual MEK-6400/6410/6420 2.23

RBC manometer upper sensor adjustment error.

RBC manometer lower sensor adjustment error.

Adjust the RBC manometer upper sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

Adjust the RBC manometer lower sensor. Refer to “Adjusting the Upper and Lower Sensor Output Voltages of the Manometers” in Section 5.

2. TROUBLESHOOTING

A061: HGB voltage low

This message appears when the HGB BLANK (diluent) voltage is less than 1.5 V.

Reason Possible Cause/Criteria Countermeasure

Deterioration from age

Erroneous operation

Photodiode does not receive the light from the LED Circuit error

WBC measurement bath is dirty. Clean or replace the WBC measurement bath with a

new one.

HGB voltage adjustment error. Adjust the HGB voltage. Refer to “Adjusting the

HGB Sensor Output Voltage” in Section 5. HGB measurement started when there was blood sample in the WBC measurement bath or the hematology analyzer was drained. There are crystals or other dust on the photodiode.

The UT-7201 HGB AMP board failure. Replace the board with a new one. The UT-7202 HGB LED board failure. Replace the board with a new one. The UT-7198 MEASURING board failure. Replace the board with a new one. The MC-640V measuring unit failure. Replace the unit with a new one.

After cleaning, perform priming and measure again.

Remove the cause.

A062: HGB voltage high

This message appears when the HGB BLANK (diluent) voltage is more than 4.5 V.

Reason Possible Cause/Criteria Countermeasure

Deterioration from age Erroneous operation

HGB voltage adjustment error. Adjust the HGB voltage. Refer to “Adjusting the

HGB Sensor Output Voltage” in Section 5.

HGB voltage is adjusted when there was

After cleaning, adjust the HGB voltage.

blood sample in the WBC measurement bath or the hematology analyzer was drained.

Circuit error

A063: HGB circuit error

This message appears when the voltage is more than 0.5 V at HGB LED off.

Reason Possible Cause/Criteria Countermeasure

Light interference The HGB cover is not attached properly. Reattach the HGB cover. Circuit error

A072: Tube holder open

The analyzer cannot operate because the tube holder is open. This message appears only on the MEK-6400 analyzer

only.

Reason Possible Cause/Criteria Countermeasure

Tube holder is open. Close the tube holderTube holder cannot

be closed.

The analyzer cannot detect if the holder is open or closed.

Holder hook does not move. Replace the MS-641V cap pierce unit with a new one.

Replace the UT-7193 POWER board with a new one. The holder sensor on the MS-641V cap pierce unit failure.

Replace the holder sensor with a new one.

Replace the UT-7193 POWER board with a new one.

2.24 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

A073: Tube in the holder

Open mode cannot be selected because the sample tube is set in the tube holder. This message appears only on the MEK-

6400 analyzer only.

Reason Possible Cause/Criteria Countermeasure

Tube in the holder The sample tube is set in the tube holder. Remove the sample tube and close the tube holder. The analyzer cannot detect the tube

A091: Room temperature high

This message appears when measurement is performed at temperatures over 30°C.

A092: Room temperature low

This message appears when measurement is performed at temperatures below 15°C.

Reason Possible Cause/Criteria Countermeasure

Room temperature high

Room temperature low The analyzer cannot measure the temperature

The tube sensor on the MS-641V cap pierce unit failure.

Measurement is performed at temperatures above 30°C. Measurement is performed at temperatures below 15°C. Sensor failure. Replace the UT-7202 HGB LED board with a

The UT-7192 AMP CONTROL board failure. Replace the board with a new one.

Replace the tube sensor with a new one. Replace the UT-7193 POWER board with a new one.

Perform measurement at temperatures between 15 to 30°C.

new one.

A093: Internal temperature high

A094: Internal temperature low

A095: Power supply temp high

This message appears when the internal temperature is abnormal.

Reason Possible Cause/Criteria Countermeasure

Internal temperature is abnormal

Actuator moves inappropriately. Circuit error. Temperature sensor failure.

Replace the electromagnetic valve, motor, unit or board which is causing the abnormal temperature.

Service Manual MEK-6400/6410/6420 2.25

2. TROUBLESHOOTING

! APPEARS ON THE RIGHT OF WBC MEASURED VALUE

! may be displayed on the right of WBC value when the RBC ghosts affect the WBC measurement. During counting, the

hematology analyzer detects the RBC pulses which are under the threshold for WBC.

Reason Possible Cause/Criteria Countermeasure

Specific characteristics of the sample

Environmental condition is not appropriate Influence from other malfunctions Measuring unit failure.

The hemolyzing reagent (HEMOLYNAC3) is not dispensed properly.

High RBC membrane resistance and many RBC ghosts.

PLT aggregation. Measure the stained sample with a microscope. Diluent temperature is low. Specified reagents are not used. Only use the specified reagents.

High background noise. Refer to the “Background Noise Problems” earlier in

WBC sensitivity and threshold settings are inappropriate. WBC aperture is not attached correctly. Reattach the WBC aperture. WBC aperture is dirty. Clean the WBC aperture. WBC aperture is damaged. Replace the WBC aperture with a new one. The screws securing the measurement baths are loose. The MC-640V measuring unit and chassis are not insulated (there may be leakage). The MC-640V measuring unit failure. Replace the unit with a new one. The UT-7198 MEASURING board failure. Replace the board with a new one. Tube is in the upper part of the tank. Put the tube in the bottom of the tank. Tube joints are loose. Tighten the tube joint. Electromagnetic valve of the MD-640V combination syringe pump unit failure. The MD-640V combination syringe pump unit failure. The UT-7200 MIXED PUMP board failure. The UT-7193 POWER board failure. Replace the board with a new one.

Dilute the sample in the pre-dilution mode and measure it in pre-dilution mode or measure the stained sample with a microscope.

Environment temperature must be from 15 to 30°C.

this section. WBC sensitivity must be 5 and threshold 4.

Tighten the screws.

Remove the cause.

Replace the unit with a new one.

Replace the board with a new one.

! APPEARS ON THE RIGHT OF MCHC MEASURED VALUE

! may be displayed on the right of MCHC value when the MCHC value is out of the normal range (lower than 28.0 or

higher than 38.0 g/dL). MCHC is known to be a stable factor, and its physiological variation is approximately 3%

independent of the patients.

Reason Possible Cause/Criteria Countermeasure

Specific

RBC aggregation. Measure with a microscope.

characteristics of the sample Environmental

Sample is hemolyzing. Measure with a microscope.

Specified reagents are not used. Only use the specified reagents.

condition is not appropriate. Calibration of the

The reagents are past the expiration date. Replace the reagents with the new ones.

Calibration of HGB or HCT is not correct. Calibrate the hematology analyzer. hematology analyzer is not correct.

No hemolysing reagent. Put the tube into the bottom of the tank.HGB measurement error

HGB sensor voltage adjustment error Adjust the HGB sensor voltage. Refer to “Adjusting

the HGB Sensor Output Voltage” in Section 5. HCT measurement error Dilution ratio error

High background noise. Refer to the “Background Noise Problems” earlier

in this section.

The MD-640V combination syringe pump

Replace the unit with a new one.

unit failure. Liquid cannot be drained from the measurement bath. Blood sampling error (sampling nozzle is

Replace the filter below the RBC measurement bath

with a new one.

Replace the sampling nozzle with a new one.

clogged, bent or air bubbles in the sampling nozzle).

2.26 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

C APPEARS ON THE RIGHT OF WBC OR PLT MEASURED VALUE or PLT Clumps appears

Platelet aggregation is a phenomenon where some platelets stick together to create a large aggregated platelet cell. This

is because some platelets in the sample excessively react against anticoagulant. When platelet aggregation occurs, the

PLT value is low and the insufficient hemolysing alarm also occurs because the aggregated PLT cell is counted as a WBC

or RBC ghost. There is a high possibility that platelet aggregation occurs when heparin is used as an anticoagulant.

Platelet aggregation rarely occurs when EDTA (ethylenediaminetetraacetic acid) salt is used. This alarm is displayed

when there is poor hemolyzation and PLT value is low.

Reason Possible Cause/Criteria Countermeasure

Specific characteristics of the sample. Error due to anticoagulant.

OTHER FLAGS

This message appears when the CBC parameters are outside the range.

Reason Possible Cause/Criteria Countermeasure

Adjustment and calibration are not appropriate

not appropriate

PLT aggregation. Measure with a microscope.

Heparin is used as an anticoagulant. Collect the sample again and use EDTA as an

anticoagulant.

EDTA dependent pseudothrombocytopenia. Collect the sample again and use heparin as an

anticoagulant.

Calibration for CBC parameters is not appropriate. Specified reagents are not used. Only use the specified reagents.Environmental condition is The environment temperature is out of 15 to 30°C.

Perform calibration.

The environment temperature must be within 15 to 30°C.

Service Manual MEK-6400/6410/6420 2.27

2. TROUBLESHOOTING

Solving Problems from System Error Messages

E001: DILUTER INITIALIZE ERROR

The MD-640V combination syringe pump unit cannot be initialized.

Reason Possible Cause/Criteria Countermeasure

Diluter piston failure

the movement of the diluter piston

E021: SAMPLER INITIALIZE ERROR

The MS-640V sampler unit cannot be initialized.

Reason Possible Cause/Criteria Countermeasure

The sampler unit does not perform the initialization

Cable(s) on the MD-640V combination syringe pump unit is damaged. Motor driver on the UT-7193 POWER board failure. Replace the board with a new one. Motor on the MD-640V combination syringe pump unit failure. The UT-7200 MIXED PUMP board sensor failure. Replace the board with a new one.Sensor cannot detect Cable(s) on the MD-640V combination syringe pump unit damaged.

Sampling nozzles do not move. Replace the sampling nozzle with a new one. The cap on the rinse unit or cap pierce unit for cap piercing catches the nozzle. Connector(s) on the sampler unit is disconnected. Cable(s) on the sampler unit is damaged. Replace the cable with a new one. Sampler initialize position sensor failure. Replace the sampler initialize position sensor with a new

Sampler separating sensor failure. Replace the sensor with a new one. Motor driver to the UT-7193 POWER board failure. Sampler motor failure. Replace the MS-640V sampler unit with a new one.

Replace the cap with a new one.

Reconnect the connector.

one.

Replace the board with a new one.

Replace the cable with a new one.

Replace the unit with a new one.

Replace the cable with a new one.

E041: SUB BATH INITIALIZE ERROR

The sub baths of the MC-640V measuring unit cannot be initialized.

Reason Possible Cause/Criteria Countermeasure

Sub baths do not rotate

detect the rotary movement of the sensor plate

The connector of the sub bath sensor is disconnected. Sub bath is not attached properly. Reattach the sub bath. Cap is not attached properly. Reattach the cap. Cable(s) on the measuring unit is damaged. Sub bath motor failure. Replace the MC-640V measuring unit with a new one. Motor driver on the UT-7193 POWER board failure. Sub bath metal plate spring which holds the sub bath spacer has rust or crystallization. Sub bath sensor on the measuring unit failure. Cable(s) on the measuring unit is damaged.

Reconnect the connector of the sub bath sensor.

Replace the MC-640V measuring unit with a new one.

Replace the board with a new one.

Remove rust or crystallization with a sandpaper. Replace the sub bath spacer with a new one if the problem still occurs. Replace the sub bath sensor with a new one.The sensor cannot

Replace the cable with a new one.

2.28 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

E101: BATH DRAIN ERROR

The MP-640V pump unit does not drain the measurement baths.

Reason Possible Cause/Criteria Countermeasure

The pump rotor does not move

the rotary movement

E122: CHECK SETTINGS

E123: MEMORY ERROR

Re-enter the calibration coefficient and all other settings. Then sample counting can be performed. If the error still

occurs, replace the battery.

Reason Possible Cause/Criteria Countermeasure

saved.

The MP-640V pump unit is disconnected from the UT-7193 POWER board. Stepping motor failure. Replace the MP-640V pump unit with a new

Motor driver on the UT-7193 POWER board failure. Pump sensor on the pump unit failure. Replace the pump sensor with a new one.The sensor cannot detect Cable(s) on the pump unit is damaged. Replace the cable with a new one.

The backup data is inappropriately changed.

Replace the CR2450 battery with a new one.The data cannot be Replace the UT-7192 AMP CONTROL board with a new one.

Reconnect or replace the cable with a new one.

one. Replace the board with a new one.

E124: CIRCUIT ERROR

Reason Possible Cause/Criteria Countermeasure

The MC-640V measuring unit failure. Replace the unit with a new one.The analyzer cannot

count preliminary pulse.

E126: TOUCH PANEL ERROR

Reason Possible Cause/Criteria Countermeasure

Touch panel does not react properly.

E141: CAP PIERCE INITIALIZE ERROR

The MS-641V cap pierce unit cannot be initialized.

Reason Possible Cause/Criteria Countermeasure

Cap pierce does not move.

The sensor cannot detect the starting position of the sample needle.

Cable(s) on the PV-640V front panel unit is damaged. Connector(s) on the front panel unit is disconnected. Touch panel failure. Replace the PV-640V front panel unit with a new one. The UT-7192 AMP CONTROL board failure. Replace the board with a new one.

The UT-7192 AMP CONTROL board failure. Replace the board with a new one.

Replace the unit with a new one.

Reconnect the connector.

Connector(s) on the MS-641V cap pierce unit is disconnected. Cable(s) on the cap pierce unit is damaged. Replace the cable with a new one.

Cap pierce motor failure.

Motor driver on the UT-7193 POWER board failure. Replace the board with a new one.

Sensor on the cap pierce unit failure. Replace the sensor with a new one.

Cable(s) on the cap pierce unit is damaged. Replace the cable with a new one.

Reconnect the connector.

Replace the MS-641V cap pierce unit with a new one.

Service Manual MEK-6400/6410/6420 2.29

2. TROUBLESHOOTING

Service Maintenance Screens

The various service maintenance screens are provided for checking the

operation of a component or block such as pump, motor, sensor and unit

individually, and for setting some functions which are usually not changed. So,

these screens are not described on the operator’s manual. There may be

additional function or change of the function which depends on the software

version. If you found a function which is not shown in this section, please

contact Nihon Kohden representative before using the function.

Using a key on one of the service maintenance screens without

reading can damage the instrument. Completely read the

corresponding part before using a screen key.

CAUTION

Displaying the SERVICE Screen

1. Press the MENU key on the READY screen. The MENU screen appears.

2. Press the SETTINGS key on the MENU screen. The SETTINGS screen

appears.

3. Press the USERS key on the SETTINGS screen. The USERS screen appears.

2.30 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

4. Select FACTORY and press the CHANGE key. A promt for the password

appears.

5. Type “4321” and press the ENTER key.

6. Press the OK key on the screen. The SETTINGS screen appears.

7. Press the OK key on the screen. The MENU screen appears.

8. Press the OTHER key on the screen. The OTHER screen appears.

9. Press the SERVICE key on the screen. The SERVICE screen appears.

The SERVICE screen has the following menu items.

• CHECK MENU

• UNIT MAINTE

• CONT MEASURE

• SETTINGS MENU

• MONITOR

• X10-CV

• INITIAL MENU

• OTHER MENU

• PRINT SETTINGS

Service Manual MEK-6400/6410/6420 2.31

2. TROUBLESHOOTING

CHECK MENU Screen

This screen allows you to check the measurement operation of the following

blocks.

WBC & RBC UNIT: Simultaneously measures each sample in the WBC and

RBC measurement baths separately from dilution

operation done by MS-640V sampler unit. So, you can

check the fluid level movement and any bubble in the

two manometers without any noise and vibration from

the other units in the instrument.

UNIT MEAS. X5: Automatically measures each sample in the WBC and

RBC measurement baths 5 times separately from

dilution operation done by MS-640V sampler unit. So,

you can check the data reproducibility without any

noise and vibration from the other units in the

instrument.

WBC UNIT MEAS.: Measures the sample in WBC measurement bath

separately from dilution operation done by MS-640V

sampler unit.

RBC UNIT MEAS.: Measures the sample in RBC measurement bath

separately from dilution operation done by MS-640V

sampler unit.

PRIME BATH: Fills the two measurement baths with diluent.

PRIME UNIT: Fills the two manometers with diluent.

CLEAN UNIT: Cleans the two manometers with detergent.

BACKUP RAM CHK: No function

UNIT MAINTE Screen

This screen allows you to check the operation of the following units and

valves.

• MD-640V Combination syringe pump unit

• MS-640V Sampler unit

• MS-641V Cap pierce unit

• MP-640V Pump unit

• Solenoid valves

2.32 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

MD-640V

This screen allows you to check the operation of the MD-640V.

CAUTION

• When pressing the DISPENSE key, place a container under the

sampling nozzle because diluent dispenses from the sampling

nozzle.

• When using the DISPENSE key or 5 TIMES AUTO key, be careful not

to let the hemolysing reagent overflow from the WBC measurement

bath.

Always perform INITIALIZE before pressing any other key.

INITIALIZE: Moves the pistons to the initial positions.

ASPIRATE: Moves the pistons to the position for aspiration.

DISPENSE: Moves the pistons to the position for dispense.

5 TIMES AUTO: Repeats the aspiration and dispense operations 5 times.

PRIME: No function.

MS-640V

This screen allows you to check the operation of the MS-640V.

CAUTION

• Before checking the operation, check that the sampling nozzle is

not bent.

• Check the sub bath positions which should be upside down

because it prevents the sampling nozzle from bending.

Always perform INITIALIZE before pressing any other key.

INITIALIZE: Moves the sampling nozzle to the initial position.

ASPIRATE POS: Moves the sampling nozzle to the position for aspirating a

blood sample

PIERCE POS: Moves the sampling nozzle to the position for the cap

pierce lowering motion.

WBC DSP POS: Moves the sampling nozzle to the position for dispensing

the diluted sample for WBC measurement.

RBC ASP POS: Moves the sampling nozzle to the position for aspirating

the diluted sample for WBC measurement.

RBC DSP POS: Moves the sampling nozzle to the position for dispensing

the diluted sample for RBC measurement.

1 CYCLE: Moves the sampling nozzle to the positions according to

each operation of MS-640V.

CONT. INITIAL: Repeats moving the sampling nozzle to the initial position

until you press the Reset key on the front panel.

Service Manual MEK-6400/6410/6420 2.33

2. TROUBLESHOOTING

MS-641V (MEK-6400 only)

This screen allows you to check the operation of the MS-641V.

CAUTION

If you check the operation while the tube holder is open, the cap

pierce needle comes out.

Always perform INITIALIZE before pressing any other key.

INITIALIZE: Moves the cap pierce needle to the initial position.

HIGH POS: Moves the cap pierce needle to the upper position.

LOW POS: Moves the cap pierce needle to the lower position.

MV & PUMP CHK

This screen allows you to check the operation of the valves and MP-640V

pump unit.

CAUTION

Before starting the pump, carefully check what happens by opening a

specified valve and working the pump. Otherwise, the reagent such

as diluent may overflow somewhere in the instrument.

When pressing a check box of MC-640V, MD-640V, JQ-640V and JQ-641V, a

check mark is placed in the check box and the valve is open. When pressing

the check box with a check mark, the check box is cleared and the valve is

closed.

There are five keys for the MP-640V pump unit. The function of each key is

shown below.

<<: High-speed clockwise rotation

<: Low-speed clockwise rotation

STOP: Stops the rotation.

>: Low-speed counterclockwise rotation

>>: High-speed counterclockwise rotation

When pressing the MONITOR key at the bottom of the screen, the SENSOR

MONITOR screen which shows each sensor status appears.

2.34 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

CONT MEASURE Screen

SETTING MENU Screen

This screen allows you to check the instrument during continuous

measurement.

NOTE

Before starting the continuous measurement, check that each reagent

volume and waste container capacity are enough to do the

continuous measurement.

10 TIMES: Continuously measures the sample 10 times.

20 TIMES: Continuously measures the sample 20 times.

30 TIMES: Continuously measures the sample 30 times.

EVER: Measures the sample until you press the Reset key on the front

panel.

The screen has settings which are not usually changed.

ADV. SETTINGS: Advanced settings

REAGENT FACTOR: Reagent consumption ratio setting

USER MAINTE: Measurement times settings for user maintenance

PANIC VALUE: Threshold settings for panic value

FLAG SETTINGS: Threshold settings for special flag display

BAR CODE PORT: Settings for RS-232C communication

INPUT UNIT CAL: Compensation value settings

ADV. SETTINGS Screen

The following settings are available.

Clean after 10th measurement:

Automatically cleans the fluid path with the detergent after every 10

measurements when this setting is set to on. To cancel this function, set this

setting to off. This setting is only for MEK-6400 and MEK-6410.

High altitude:

Makes the measurement time longer when this setting is set to on. When the

instrument is installed at a place lower than 1000 m above sea level, check that

this setting is set to off.

Using handy bar code in measurement:

Allows input of sample ID number with a handy bar code reader when this

setting is set to on.

Histogram smoothing:

Smooths the histogram when this setting is set to on. The default setting is on.

Service Manual MEK-6400/6410/6420 2.35

2. TROUBLESHOOTING

Alarm recount:

When this setting is set on and an alarm message such as bubble or noise

occurs, the instrument automatically measures the sample up to 3 times while

displaying the alarm message for each measurement. This function is useful to

check what alarm occurs during the recount if the recount frequently occurs.

The default setting is off.

REAGENT FACTOR Screen

This screen allows you to change the ratio of consumption of each reagent

when there is a difference between the actual consumption and calculated

consumption on the screen.

Diluent: Changes the consumption rate of the diluent.

Detergent: Changes the consumption rate of the detergent.

Lysing reagent: Changes the consumption rate of the hemolysing reagent

USER MAINTENANCE Screen

This screen allows you to change the measurement times of the following

items.

The instrument counts the measurement times. When the actual measurement

times exceeds the preset measurement times for check, cleaning or replacement,

the corresponding user maintenance message appears.

Filters, Baths: Measurement times for checking, cleaning or replacing the

filter and measurement and sub baths.

Pump tube: Measurement times for checking, cleaning or replacing the

pump tube.

Rinse unit: Measurement times for checking, cleaning or replacing the

rinse unit.

Sampling nozzle: Measurement times for checking, cleaning or replacing the

sampling nozzle.

Cap pierce nozzle: Measurement times for checking, cleaning or replacing the

cap pierce needle.

2.36 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

PANIC VALUE Screen (for MEK-6400 and MEK-6410)

This screen allows you to change the upper and lower thresholds for

remeasurement with the dilution ratio changed.

When a blood sample has a parameter which is out of the range between the

upper and lower thresholds, the instrument automatically measures the sample

with the dilution ratio changed. Changing the upper and lower thresholds

depends on the user’s needs.

FLAG SETTINGS Screen

This screen allows you to change the thresholds for display of the following

specified flags.

If there are a lot of normal blood samples which have one of the following flags

frequently displayed, increase the value to prevent displaying the flag.

Anisocytosis: The initial value is 20.0 (RDW)

PLT clumps: The initial value is 300.

Plt-Rbc interface: The initial value is 0.10

BAR CODE PORT Screen

This screen allows you to set the settings for serial data communication when

the bar code port is used as RS-232C port.

Use handy bar code reader: Check that a check mark is place in the check

box aside of “Yes” when using a handy bar code

reader. The default setting is “Yes”.

Use specified cable: Press the check box aside of “Yes” when using

this port as RS-232C port with the YZ-0319

cable. The default setting is “No”.

Service Manual MEK-6400/6410/6420 2.37

2. TROUBLESHOOTING

MONITOR Screen

UNIT CAL Screen

This screen allows you to change the compensation values of the cylinder

volume of the MD-640V combination syringe pump unit and manometer

volumes for RBC and WBC of the MC-640V measuring unit. When replacing

the MD-640V or MC-640V unit with a new one, the change of the

compensation value is not necessary because the compensation value of the

new unit is automatically registered. When you replace the board of the MD-

640V or MC-640V unit, i.e. UT-7200 or UT-7198, with a new one, you need to

change the compensation value. Before the board replacement, write down the

compensation value. Enter it after the board replacement.

This screen allows you to check the output voltage from each sensor, alarm log

and system error log.

• SENSOR MONITOR screen:

Displays the status of all sensors with voltage or temperature.

For details on the INPUT CAL key, refer to Section 5 “Adjustment”.

• POS SENSOR screen:

No function

2.38 Service Manual MEK-6400/6410/6420

2. TROUBLESHOOTING

• ALARM LOG screen:

Displays all the alarm data with date and time if they occur. You can get the

problem tendency using this log data. For example, if some “manometer

dirty” message occurs frequently, you can try to perform the strong cleaning.

• SYSTEM ERR LOG screen:

Displays all the system error data with date and time so you can see any

problem trends.

X10-CV screen

This screen allows you to check the data reproducibility of the latest 10

measurement data.

After the instrument is repaired or the measurement results are unstable, use this

function. If the latest 10 measurements include unnecessary data, delete the

unnecessary data beforehand.

Service Manual MEK-6400/6410/6420 2.39

2. TROUBLESHOOTING

INITIAL MENU Screen

This screen allows you to initialize specified parts or all data in the instrument.

• CALIBRATION: Sets all the calibration coefficients to 1000.

• MEASURED DATA: Deletes all the measurement data.

• QC DATA: Deletes all the measurement data for quality control and

initializes all the settings for quality control.

• SETTINGS: Initializes all the settings on the SETTINGS screen. This

function is same as the “INITIALIZE” key at the bottom of the SETTINGS

screen.

• USER COUNT: Resets all the counters for regular replacement parts to zero.

• MAINTENANCE: Initializes all the settings on the service maintenance

screens.

• FACTORY SET: Initializes all the above mentioned settings to factory

default settings and deletes all the above mentioned data.

OTHER MENU Screen

PRINT SETTINGS Screen

This screen allows you to download the demonstration data from the PC

memory card when inserting the memory card into the slot at the rear panel of

the instrument and pressing the “DOWNLOAD DATA” key.

No function

2.40 Service Manual MEK-6400/6410/6420

Section 3 Board/Unit Description

Board and Unit Location ..................................................................................................................................... 3.3

Right Side View........................................................................................................................................ 3.3

Left Side View .......................................................................................................................................... 3.3

Front View ................................................................................................................................................ 3.4

Top View .................................................................................................................................................. 3.4

MC-640V Measuring Unit ................................................................................................................................... 3.5

MD-640V Combination Syringe Pump Unit ......................................................................................................... 3.5

MP-640V Pump Unit .......................................................................................................................................... 3.6

PV-641V Front Panel Unit ................................................................................................................................... 3.6

JQ-640V Inlet/Outlet Unit ................................................................................................................................... 3.7

JQ-641V/JQ-642V Valve Unit ............................................................................................................................. 3.7

JQ-641V Valve Unit .................................................................................................................................. 3.7

JQ-642V Valve Unit .................................................................................................................................. 3.7

MS-640V Sampler Unit....................................................................................................................................... 3.8

MS-641V Cap Pierce Unit (MEK-6400 only) ....................................................................................................... 3.8

MR-640V Rinse Unit (MEK-6410/6420 only) ....................................................................................................... 3.9

UT-7192 AMP CONTROL Board ......................................................................................................................... 3.9

UT-7193 POWER Board .................................................................................................................................... 3.10

UT-7198 MEASURING Board ............................................................................................................................ 3.10

UT-7201 HGB AMP Board and UT-7202 HGB LED Board ..................................................................................3.11

UT-7200 MIXED PUMP Board ...........................................................................................................................3.11

UT-7205 PRINTER DRIVER Board .................................................................................................................... 3.12

UT-7203 KEY Board .......................................................................................................................................... 3.12

UT-7199 LIQUID SENSOR Board ...................................................................................................................... 3.13

XP-602V/XP-612V 2-way Electromagentic Valve .............................................................................................. 3.13

Service Manual MEK-6400/6410/6420 3.1

3. BOARD/UNIT DESCRIPTION

This section describes the following units and boards and how they work

during operation. This information helps you to fully understand the overall

operation of the instrument.

• Measuring unit

• Combination syringe pump unit

• Pump unit

• Front panel unit

• Inlet/Output unit

• Valve unit

• Sampler unit

• Cap pierce unit (MEK-6400 only)

• AMP CONTROL board

• POWER board

• MEASURING board

• HGB AMP board and HGB LED board

• MIXED PUMP board

• PRINTER DRIVER board

• KEY board

• LIQUID SENSOR board

• 2-way electromagentic valve

3.2 Service Manual MEK-6400/6410/6420

Board and Unit Location

3. BOARD/UNIT DESCRIPTION

Right Side View

Front Panel unit

Cap pierce unit (MEK-6400 only)

Sampler unitValve unit

Measuring unit

Inlet/Outlet unit

Combination syringe pump unit

Pump unit

MIXED PUMP board

Left Side View

AMP CONTROL board

MEASURING board

Service Manual MEK-6400/6410/6420 3.3

3. BOARD/UNIT DESCRIPTION

Front View

LCD

KEY board

PRINTER DRIVER board

Top View

AMP CONTROL board

LIQUID SENSOR board

POWER board

3.4 Service Manual MEK-6400/6410/6420

MC-640V Measuring Unit

When a blood cell of the diluted blood sample passes through the aperture, the

resistance between the external and internal electrodes increases. This change

in resistance causes a proportional change in voltage because a constant

current flows between the electrodes. This measuring unit counts the blood

cells by the voltage changes. Meanwhile, the total diluted blood sample

volume which is aspirated through the aperture is kept constant by the

manometer of this measuring unit.

At the lower part of the WBC measurement bath, a light of 540 nm wavelength

shines through the hemolyzed blood sample. The sample absorbs some of the

light. A photodiode detects the unabsorbed light. The HGB can be determined

from the light absorption ratio of the sample and diluent.

3. BOARD/UNIT DESCRIPTION

MD-640V Combination Syringe Pump Unit

This unit dilutes the blood sample 200 times and sends the diluent to each part

of the fluid path. This unit has another syringe pump for hemolyzing reagent

which aspirates the hemolyzing reagent from the reagent bottle and sends it to

the WBC measurement bath.

Service Manual MEK-6400/6410/6420 3.5

3. BOARD/UNIT DESCRIPTION

MP-640V Pump Unit

This unit creates a negative pressure to aspirate diluted blood through each

aperture, and to drain the waste fluid. This unit creates a positive pressure to

completely mix the diluted blood sample and hemolyzing reagent for

hemolyzation.

PV-641V Front Panel Unit

This unit has key switches and LCD with touch screen. It may also have an

optional printer unit. The LCD displays the measurement result (numeric data

and histogram) and alarm message if something wrong occurs. The

measurement result can be printed if an optional printer is installed.

3.6 Service Manual MEK-6400/6410/6420

JQ-640V Inlet/Outlet Unit

This unit has inlet ports for diluent, detergent and hemolyzing reagent and an

outlet port for waste fluid. The three liquids which are detected with liquid

sensors of this unit are supplied to the related units through the fluid path. The

waste fluid is drained through the outlet port.

3. BOARD/UNIT DESCRIPTION

JQ-641V/JQ-642V Valve Unit

JQ-641V Valve Unit

This unit is for the MEK-6400 analyzer. It has six 2-way valves and a fluid path

to send the diluent or detergent to the other units.

JQ-642V Valve Unit

This unit is for the MEK-6410/MEK-6420 analyzers. It has four 2-way valves

and a fluid path to send the diluent or detergent to the other units.

Service Manual MEK-6400/6410/6420 3.7

3. BOARD/UNIT DESCRIPTION

MS-640V Sampler Unit

This unit moves the two sampling nozzles to the positions for aspiration and

dispensing. The diluted sample is dispensed into the WBC and RBC

measurement baths by coordination between this sampler unit and the syringe

pump unit.

MS-641V Cap Pierce Unit (MEK-6400 only)

This unit pierces the cap of a sample tube with a cap pierce needle to aspirate

the blood sample with the sampling nozzle (without opening the cap of the

sample tube). This unit also has a cap pierce rinse unit to clean the cap pierce

needle.

3.8 Service Manual MEK-6400/6410/6420

MR-640V Rinse Unit (MEK-6410/6420 only)

This unit cleans the sampling nozzles.

3. BOARD/UNIT DESCRIPTION

UT-7192 AMP CONTROL Board

This board consists of two blocks. One block amplifies the analog signals from

the measuring unit and converts the amplified analog signals to digital signals.

The other block controls the valves and motors while receiving the key switch

status and signals from the sensors.

This board controls all the operations such as cleaning and measurement.

Service Manual MEK-6400/6410/6420 3.9

3. BOARD/UNIT DESCRIPTION

UT-7193 POWER Board

This board converts AC line voltage to the necessary DC voltages through the

power transformer. This board also has all the motor driver ICs to control the

stepping motor(s) and valve(s) of each unit.

UT-7198 MEASURING Board

This board which is attached to the MC-640V measuring unit sends the voltage

between the electrodes, liquid level sensor output voltages and HGB sensor

output voltages to the UT-7192 AMP CONTROL board.

3.10 Service Manual MEK-6400/6410/6420

3. BOARD/UNIT DESCRIPTION

UT-7201 HGB AMP Board and UT-7202 HGB LED Board

These boards which are attached to the MC-640V measuring unit sends the

HGB optical density to the UT-7198 MEASURING board.

UT-7201 UT-7202

UT-7200 MIXED PUMP Board

This board which is attached to the MD-640V combination syringe pump unit

sends the position sensor output signal of the diluter piston to the UT-7193

POWER board.

Service Manual MEK-6400/6410/6420 3.11

3. BOARD/UNIT DESCRIPTION

UT-7205 PRINTER DRIVER Board

This board which is attached to the WA-640VK printer unit receives the printer

control signals from the UT-7192 AMP CONTROL board to drive the motor and

thermal array head of the printer unit.

UT-7203 KEY Board

This board which is attached to the PV-640VK front panel unit sends the status

signals of the key switches on this board to the UT-7192 AMP CONTROL

board.

3.12 Service Manual MEK-6400/6410/6420

UT-7199 LIQUID SENSOR Board

This board which is attached to the JQ-640V inlet/outlet unit has two liquid

sensors for detection of diluent, detergent and hemolyzing reagent.

3. BOARD/UNIT DESCRIPTION

XP-602V/XP-612V 2-way Electromagentic Valve

These valves open or close the fluid path when receiving the valve drive

voltage from the UT-7193 POWER board. XP-602V valve has a silicone rubber

diaphragm. XP-612V valve has a fluorocarbon rubber diaphragm.

Service Manual MEK-6400/6410/6420 3.13

4. DISASSEMBLY AND ASSEMBLY

Section 4 Disassembly and Assembly

Before You Begin ................................................................................................................................................ 4.2

Warnings and Cautions ............................................................................................................................ 4.2

Required Tools .......................................................................................................................................... 4.2

Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the Instrument ........................ 4.3

Board and Unit Location ........................................................................................................................... 4.3

Turning the Power Off ......................................................................................................................................... 4.4

Cleaning and Draining the Fluid Pathway .................................................................................................. 4.4

Turning the Power Off ............................................................................................................................... 4.5

Removing the Right Side Cover, Top Cover and Rear Cover ............................................................................... 4.6

Reattaching the Right Side Cover, Top Cover and Rear Cover .................................................................. 4.7

Removing the Front Panel Unit ........................................................................................................................... 4.8

Reattaching the Front Panel Unit .............................................................................................................. 4.9

Installing the WA-640VK Printer Unit (Option) ....................................................................................................4.10

Removing the Measuring Unit ........................................................................................................................... 4.12

Reattaching the Measuring Unit ..............................................................................................................4.13

Accessing the Connectors on the POWER Board ............................................................................................. 4.14

Reassembling the Board ......................................................................................................................... 4.14

Removing the Combination Syringe Pump Unit ................................................................................................. 4.15

Reattaching the Combination Syringe Pump Unit .................................................................................... 4.16

Removing the Pump Unit ..................................................................................................................................4.17

Reattaching the Pump Unit ..................................................................................................................... 4.18

Removing the Sampler Unit ............................................................................................................................... 4.19

Reattaching the Sampler Unit ................................................................................................................. 4.22

Removing the Cap Pierce Unit or Rinse Unit ..................................................................................................... 4.23

Reattaching the Cap Pierce Unit or Rinse Unit ........................................................................................ 4.24

Removing the Inlet/Outlet Unit .......................................................................................................................... 4.25

Reattaching the Inlet/Outlet Unit ............................................................................................................. 4.26

Removing the Valve Unit ...................................................................................................................................4.26

Reattaching the Valve Unit ...................................................................................................................... 4.27

Removing the AMP CONTROL Board ............................................................................................................... 4.28

Reattaching the AMP CONTROL Board .................................................................................................. 4.29

Removing the POWER Board ........................................................................................................................... 4.30

Reattaching the POWER Board ..............................................................................................................4.31

Removing the MEASURING Board ...................................................................................................................4.32

Reattaching the MEASURING Board ...................................................................................................... 4.33

Removing the MIXED PUMP Board ..................................................................................................................4.34

Reattaching the MIXED PUMP Board .....................................................................................................4.34

Removing the PRINTER DRIVER Board ........................................................................................................... 4.35

Reattaching the PRINTER DRIVER Board .............................................................................................. 4.35

Removing the KEY Board .................................................................................................................................4.36

Reattaching the KEY Board .................................................................................................................... 4.36

Removing the LIQUID SENSOR Board ............................................................................................................. 4.37

Reattaching the LIQUID SENSOR Board ................................................................................................ 4.37

Removing the LCD ............................................................................................................................................ 4.38

Reattaching the LCD ............................................................................................................................... 4.39

Removing the Transformer ................................................................................................................................. 4.40

Reattaching the Transformer....................................................................................................................4.40

Service Manual MEK-6400/6410/6420 4.1

4. DISASSEMBLY AND ASSEMBLY

Before You Begin

Warnings and Cautions

The procedures in this section tell how to remove, replace and install major

components in the instrument.

Only qualified service personnel should remove, replace and install major

components.

WARNING

• To avoid the possibility of injury to yourself or damage to the

instrument, do not install or remove any component or change

switch settings while the power is on and wait 10 minutes before

installing to or removing any component from the instrument after

the power is off.

• To avoid accidental discharge of static electricity which could

damage the instrument components, use a wrist ground strap when

installing or removing any component of the instrument.

• When replacing any parts or units in the instrument, do not touch

any part of the instrument where blood is or may have contacted.

• Wear rubber gloves to prevent infection by blood.

Required Tools

CAUTION

• Before connecting or disconnecting any cables, turn off the

instrument and unplug the AC power cord from the instrument.

• Fuses cut off the power when an abnormality occurs in the

instrument. Eliminate the malfunction before replacing the fuse.

Use the correct fuse only. The fuse rating is shown on the holder.

• Removal and replacement of any component in the instrument

should be done by qualified service personnel.

• Only use parts recommended by Nihon Kohden to assure

maximum performance from your instrument.

• Anti-static bench mat

• Wrist ground strap

• Phillips screwdriver (insulated type)

• Flat-blade screwdriver (insulated type)

• Hex socket driver

• Tweezers

4.2 Service Manual MEK-6400/6410/6420

4. DISASSEMBLY AND ASSEMBLY

Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the Instrument

CAUTION

Valve Joint

When connecting the valve joint to the electromagnetic valve, turn

the valve joint clockwise, using moderate force until the valve joint

comes to a stop. Do not use extreme force to tighten the valve joint

further because this will damage the tip of the valve joint. If the

valve joint is loosely connected, it will leak.

NOTE

Black screw

Black screws are used to fasten the individual units to the chassis of

the instrument to enable the quick removal and replacement of these

units. However, to fasten the pump unit to the chassis, normal

screws are used.

Spring type tube joint

• Spring type tube joints are used in the instrument to prevent over-

tightening of the joints, and to prevent loosening of the joints after

the joints are tightened.

• There are 2 types of spring tube joints, white (inlet side) and black

(outlet side). Each tube joint and its corresponding port in the

instrument are marked with the same color or number to ensure

matching.

No spring type tube joint

The no spring type tube joint consists of an O-ring, tube stopper and

fitting nut. To disconnect the tube from this joint, turn the fitting nut

counterclockwise to loosen the joint and pull the tube toward you.

To reconnect the tube to this joint, insert the tube into the fitting nut

and turn the fitting nut clockwise to fasten it.

O-ring

Tube stopper

Fitting nut

Tube

Board and Unit Location

Refer to “Board and Unit Location” in Section 3.

Service Manual MEK-6400/6410/6420 4.3

4. DISASSEMBLY AND ASSEMBLY

Turning the Power Off

Before turning the analyzer power off, perform strong cleaning and drain the

fluid from the instrument.

Cleaning and Draining the Fluid Pathway

1. Press the OPERATIONS key on the MENU screen to display the

OPERATIONS screen.

2. Press the STRONG CLEAN key on the OPERATIONS screen.

3. Replace the CLEANAC detergent with CLEANAC•3 detergent. Refer to

“Connecting Tubes and Installing Reagents” in Section 2 of the Operator’s

Manual.

4. Press the YES key to perform strong cleaning. The analyzer starts cleaning

and the “Strong cleaning” message appears on the screen.

After cleaning, the instruction to replace the CLEANAC•3 detergent with

CLEANAC detergent appears.

5. Ignore the message and press the OK key. The screen returns to the READY

screen.

4.4 Service Manual MEK-6400/6410/6420

Waste container

4. DISASSEMBLY AND ASSEMBLY

6. Remove the diluent tube from the ISO3 diluent inlet and the hemolysing

reagent tube from the HEMO3N inlet on the right side panel. Remove the

detergent tube from the detergent container and put it into the waste

container.

Do not disconnect the waste tube from the WASTE outlet.

NOTE

• Make sure that the waste tube is correctly connected.

• Waste comes out from the CLN inlet when DRAIN ALL is performed.

• To handle the diluent, detergent, hemolysing reagent and waste

containers, follow the instructions on each package label.

7. Press the OPERATIONS key on the MENU screen to display the

OPERATIONS screen.

8. Press the DRAIN ALL key on the OPERATIONS screen. A confirmation

message appears.

Turning the Power Off

9. Press YES to start draining. During draining, the screen shows the

“Draining” message.

After draining, the screen returns to the READY screen.

CAUTION

Turn off the main power before doing maintenance. Otherwise, the

operator may receive electrical shock.

Press the [Power] key while holding down the [Reset] key. The power turns off

without cleaning. Check that the power lamp is off.

Service Manual MEK-6400/6410/6420 4.5

4. DISASSEMBLY AND ASSEMBLY

Removing the Right Side Cover, Top Cover and Rear Cover

WARNING

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the 3 screws on the rear panel and one screw on the right side

panel. The two screws marked with A only need to be loosened and not

removed.

3. Slide the right side cover to the rear direction and then outward to remove

the cover from the chassis.

4. Remove the 4 screws which secure the top cover to the chassis.

4.6 Service Manual MEK-6400/6410/6420

4. DISASSEMBLY AND ASSEMBLY

5. Pull the top cover rearward and remove it.

6. Loosen the two screws at the bottom of the rear cover.

7. Remove the 5 screws from the rear cover.

8. Remove the rear cover by tilting it rearward and then lifting it up.

Reattaching the Right Side Cover, Top Cover and Rear Cover

To reattach the right side cover, top cover and rear cover, reverse the above

procedure.

Loosen these two screws

Service Manual MEK-6400/6410/6420 4.7

4. DISASSEMBLY AND ASSEMBLY

Removing the Front Panel Unit

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the right side cover and top cover. Refer to “Removing the Right

Side Cover, Top Cover and Rear Cover” earlier in this section.

3. Remove the 3 cable connectors from the AMP CONTROL board. The

lowest cable is connected between the AMP CONTROL board and printer

when the optional WA-640VK printer unit is installed in the instrument.

WARNING

AMP CONTROL board

Printer unit cable

4. Remove the cable connector (red cable) from the POWER board.

LIQUID SENSOR board

POWER board

Cable connector

4.8 Service Manual MEK-6400/6410/6420

4. DISASSEMBLY AND ASSEMBLY

Upper coupling

Rinse unit

Sampling nozzles

5. Check the sampling nozzle position. Make sure that the sampling nozzle is

up and not inserted in the rinse unit. If the sampling nozzle is lowered and

in the rinse unit, the sampling nozzle may be broken when the front panel

unit is removed. To raise the sampling nozzle to the top position, rotate the

upper coupling counterclockwise (when viewed from the front).

6. Remove the 4 screws which secure the front panel unit to the chassis.

There are 2 screws on the

same position on the right side

of the front panel.

7. Carefully pull the front panel unit frontward while checking that the cables

are not caught. Completely separate the front panel unit from the chassis.

Reattaching the Front Panel Unit

To reattach the front panel unit, reverse the above procedure.

Service Manual MEK-6400/6410/6420 4.9

4. DISASSEMBLY AND ASSEMBLY

Installing the WA-640VK Printer Unit (Option)

WARNING

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the right side cover and top cover. Refer to “Removing the Right

Side Cover, Top Cover and Rear Cover” earlier in this section.

3, Remove the front panel unit. Refer to “Removing the Front Panel Unit”

earlier in this section.

4. Remove the 8 screws which secure the blank panel to the front panel unit

and remove the blank panel.

Blank panel

5. Remove the 2 screws which secure the printer blank panel to the backside

of the front cover.

6. Remove the printer blank panel from the front side of the front panel unit

while depressing the stopper downward and pulling the printer blank panel

as shown left.

Stopper

4.10 Service Manual MEK-6400/6410/6420

4. DISASSEMBLY AND ASSEMBLY

7. Attach the optional WA-640VK printer unit without printer door to the

front cover with 8 screws. During this step, check that no cable is pinched.

8. Put the front panel unit back to the original position.

9. Fasten the front panel unit to the chassis with the 4 screws.

10. Connect the 2 cables of the front panel unit to the AMP CONTROL board.

11. Connect the cable of the printer unit to the AMP CONTROL board.

Front panel unit cables

Printer unit cable

12. Connect the cable of the front panel unit to the POWER board. (Refer to

step 4 in the “Removing the Front Panel Unit” section.)

13. Attach the printer door to the printer unit on the front side of the front

panel unit while depressing the stopper downward and pushing the printer

door into the opening of the printer unit.

Service Manual MEK-6400/6410/6420 4.11

4. DISASSEMBLY AND ASSEMBLY

Removing the Measuring Unit

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the right side cover and top cover. Refer to “Removing the Right

Side Cover, Top Cover and Rear Cover” earlier in this section.

3. Disconnect the 3 cables from the AMP CONTROL board.

WARNING

4. Loosen the 4 screws which secure the measuring unit to the chassis.

Screw

4.12 Service Manual MEK-6400/6410/6420

4. DISASSEMBLY AND ASSEMBLY

5. Remove the 4 tube joints and sub bath motor cable connector.

CAUTION

Be careful not to cut your finger with the edges of the chassis.

Sub bath motor cable connector

Tube joint

6. Pull out the measuring unit toward you.

Reattaching the Measuring Unit

To reattach the measuring unit, reverse the above procedure.

When reattaching the measuring unit, place the measuring unit to the

right as far as possible.

NOTE

Service Manual MEK-6400/6410/6420 4.13

4. DISASSEMBLY AND ASSEMBLY

Accessing the Connectors on the POWER Board

WARNING

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the right side cover, top cover and rear cover. Refer to “Removing

the Right Side Cover, Top Cover and Rear Cover” earlier in this section.

3. Remove the 2 screws which secure the POWER board to the chassis.

Screw

4. Open the board to the right. If it is difficult to open the board, pull out the

chassis (marked A).

Reassembling the Board

To assemble the board, reverse the above procedure.

4.14 Service Manual MEK-6400/6410/6420

4. DISASSEMBLY AND ASSEMBLY

Removing the Combination Syringe Pump Unit

WARNING

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the right side cover, top cover and rear cover. Refer to “Removing

the Right Side Cover, Top Cover and Rear Cover” earlier in this section.

3. Loosen the 2 screws (A and B) which secure the combination syringe pump

unit to the chassis.

Screw A

Screw B

4. Remove the 5 tube joints from the combination syringe pump unit.

Screw A

Tube joint 4

Tube joint 1

Tube joint 5

Screw B

Tube joint 2

Service Manual MEK-6400/6410/6420 4.15

Tube joint 3

4. DISASSEMBLY AND ASSEMBLY

Cable connected to stepping motor

MIXED PUMP board

Cable connected to MIXED PUMP board

5. Remove the cable connector from the MIXED PUMP board and stepping

motor.

Tube joint 5

6. Pull out the combination syringe pump unit by tilting it toward you. Be

careful not to damage any tube.

Reattaching the Combination Syringe Pump Unit

To reattach the combination syringe pump unit, reverse the above procedure.

4.16 Service Manual MEK-6400/6410/6420

Removing the Pump Unit

For your safety, before disassembling the hematology analyzer, wait

10 minutes after turning off the main power switch. Remove all

cables and cords connected to the instrument.

1. Do the procedure in “Turning the Power Off” earlier in this section to turn

off the instrument.

2. Remove the right side cover, top cover and rear cover. Refer to “Removing

the Right Side Cover, Top Cover and Rear Cover” earlier in this section.

3. Remove the cable connector from the POWER board (blue and yellow

cables). To access the POWER board, refer to “Accessing the Connectors on

the POWER Board” earlier in this section.

4. DISASSEMBLY AND ASSEMBLY

WARNING

POWER board

Cable connector

4. Remove the cable connector from the photo sensor. Remove all cable

clamps from this cable.

Screw

Cable connector

5. Remove the screw and pull out the photo sensor.

Service Manual MEK-6400/6410/6420 4.17

4. DISASSEMBLY AND ASSEMBLY

6. Remove the 3 screws which secure the pump unit to the chassis. The screws

are guarded by blue rubber rings.

7. Remove the 2 tube joints from the pump unit.

Tube joint

8. Pull out the pump unit toward you.

Tube joint

Screw

Screws

Reattaching the Pump Unit

To reattach the pump unit, reverse the above procedure.

4.18 Service Manual MEK-6400/6410/6420

Nihon-Kohden Celltak MEK-6400 Service manual

Specifications and Main Features

Frequently Asked Questions

User Manual