Metrohm 753 User Manual
METROHM Ltd. CH-9101 Herisau (Switzerland)
753 Suppressor Module
8.753.1023 Supplement to 8.753.1003 Instructions for Use
This supplement contains corrections and supplements to the 8.753.1003 Instructions for
Use. All page numbers quoted refer to said 753 Instructions for Use.
Regeneration of the suppressor p. 29
The following instructions on how to regenerate the Metrohm Suppressor Module replace section 4.2.5 of the 753 Instructions for Use:
Regenerating a suppressor operating at reduced capacity
If the suppressor units are exposed to certain heavy metals (e.g. iron) or organic
contaminants for long periods of time, these can no longer be removed completely
by the regeneration solution normally used (20 mmol/L H
). This diminishes the
2SO4
capacity of the suppressor units, which, in milder cases, results in reduced sensitivity to phosphates and, in severe cases, in a strong increase in the baseline. If such
capacity problems occur at one or more points, treat the suppressor units affected
as follows:
1 Disconnect suppressor from IC system
• Disconnect suppressor from separating column and detector.
2 Regenerate suppressor
• Rinse each suppressor unit for about 15 min with one of the following
solutions:
Contamination with heavy metals
1 mol/L H
2SO4
Contamination with organic cationic complexing agents
0.1 mol/L H
/ 0.1 mol/L oxalic acid / acetone 5%
2SO4
Severe contamination with organic substances
0.2 mol/L H
/ acetone ≥ 20%
2SO4
The 6.1826.050 pump tubing is made of PVC and must not be used
for rinsing with solutions containing acetone. In such cases, use a
different pump tubing or a different pump for rinsing purposes.
3 Connect suppressor to IC system
• Reconnect suppressor to the IC system. If the capacity problems persist,
replace the suppressor rotor (see section 4.2.7).
753 Suppressor Module, 8.753.1023
1
Regenerating the suppressor in case of high counterpressure
If excessive counterpressure is observed in one or more of the suppressor units,
proceed as follows:
1 Disconnect suppressor from IC system
• Disconnect suppressor from separating column and detector.
2 Regenerate suppressor
• Connect the inlet capillary 25 marked "H
" to the 709 IC Pump using a
2SO4
6.2620.060 or 6.2744.040 coupling.
• Adjust the 709 IC Pump flow rate to 0.5 mL/min and rinse the suppressor
unit with 1 mol/L H
for 5 to 10 min.
2SO4
• As the pressure drops, slowly increase the 709 IC Pump flow rate to
2 mL/min. The maximum pressure must not exceed 2 MPa (20 bar).
• Switch suppressor to the next position using the <STEP> key.
• Connect the inlet capillary 28 marked "H
O" to the 709 IC Pump using a
2
6.2620.060 or 6.2744.040 coupling.
• Adjust the 709 IC Pump flow rate to 0.5 mL/min and rinse the suppressor
unit with distilled H
O for 5 to 10 min.
2
• As the pressure drops, slowly increase the 709 IC Pump flow rate to
2 mL/min. The maximum pressure must not exceed 2 MPa (20 bar).
3 Connect suppressor to IC system
• Reconnect the suppressor to the IC system and the 753 Suppressor
Module. If the pressure problems persist, replace the suppressor rotor
(see section 4.2.7).
Supplement to «Cleaning the suppressor» p. 30
Supplementary to the cases listed in section 4.2.6 which may necessitate cleaning of
the suppressor or replacement of connection piece 36, please also note the following
case:
• High counterpressure on the suppressor connection
tubings
Changes in standard equipment p. 37
2 × 6.2744.010 PEEK Compression fitting (instead of 1 × 6.2744.010)
Set of 5
2 × 6.2128.180 Connecting cable (instead of 1 × 6.2128.090)
Connecting cable 762 – 752/753/754
2 × 6.2821.100 Filter unit PEEK 2 µm (new)
To avoid contamination due to abrasive particles of piston
seals
New Options p. 38
6.2143.220 Connecting cable
Connecting cable 732 IC Detector – 753 Suppressor Module –
754 Dialysis Unit
2
753 Suppressor Module, 8.753.1023
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