Leica DM IL User Manual

Leica DM IL LED
Brilliant Optics Combined with Innovative Illumination
The New Inverted Microscope for Routine and Laboratory Microscopy in Cell Biology and Medicine
Inverted Routine
Microscopy in a New Light
Compact and stable
Lean and sturdy design• Plenty of space for operation• Low stage height• Large dimensions and low
center of gravity of microscope Large working distances
Cell biology and medicine• Micromanipulation (injection, IVF, ICSI)• Medicine• Biotechnology• Developmental biology• Transgenics
Optical performance and illumination are key elements in micros­copy. Both characteristics are unifi ed in the new design of the Leica DM IL LED. As the fi rst inverted routine microscope, the Leica DM IL LED is not only equipped with outstanding Leica HC optics, but also features innovative LED illumination. The transmit­ted-light illuminator including optimized condensers and improved contrast methods are adapted specifi cally for cell biology appli­cations. High stability, plenty of space for operation, large working distances, illumination without heat development and the sepa­rately accommodated electronics provide optimum conditions for microscopy. The Leica DM IL LED is exceptionally well-suited for uses ranging from various cell and tissue culture examinations in life sciences, developmental biology studies or micromanipulation in cell biology to living cell examinations in transgenics or electro­physiology.
Molecular biology• Fluorescence applications
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The fl uorescence version, the Leica DM IL LED Fluo, also offers a variety of possible applications. Optionally, it is also available with the new LED illumination.
Integrated fl uorescence
Manual fl uorescence with three fi lter cubes
Heatable microscope stages and 3-plate cross-stages provide great fl exibility for experiments on living cells under physiological conditions.
The Leica DM IL LED has a further advantage that distinguishes it from other microscopes in its class: The stand is highly com­patible with components of the Leica research microscopes. Objectives, eyepieces, tubes, camera ports, contrast methods. Additionally, special tubes and condensers have been developed for the Leica DM IL LED.
Integrated shutter• Optionally LED, classic mercury
illumination or fi ber optic coupling
Flexible and modular
A full range of optical components• Compatible with research stands • Unheated and heated stages• Large selection of tubes • Comprehensive range of accessories
for special applications
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The Most Comprehensive Array
of Contrast Methods
All available contrast methods can be adapted to individual applications easily and quickly. Two condensers have been devel­oped specifi cally for the Leica DM IL LED, which can be used for the entire magnifi cation range of the respective contrast method. The high-resolution S40/0.45 condenser makes even tiny details of a specimen visible. Both condensers, the S40/0.45 and S80/0.30, allow for use of phase contrast up to the 63x objective as well as Integrated Modulation Contrast (IMC) up to the 40x objective.
Fatigue-free operation
The ergonomic arrangement of all controls such as the focus dial, brightness controller, condenser height adjustment, objective nosepiece and XY stage adjustment allow users to be relaxed while working with the microscope – even for hours. The height­adjustable stages, Ergo tubes with variable tube height, fl exible viewing height, and the interpupillary distance and diopter setting enable each user to confi gure his or her personal Leica DM IL LED. The large working distances provide suffi cient room for large cul­ture fl asks, and the unobstructed view of the specimen area fa­cilitates handling more diffi cult specimens.
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