Leica DM4000M, DM4000B User Manual

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Leica DM4000 B

Leica DM4000 M

Leica DM5000 B

Operating Manual

1

Published 2003 by:

Leica Microsystems Wetzlar GmbH

Ernst-Leitz-Straße

D-35578 Wetzlar (Germany)

Responsible for contents: Katja Peter, Karin Schwab

Marketing CM, Compound Microscopy, Product Management

In case of questions, please contact:

Phone +49(0)6441-292261

Fax +49(0)6441-292255

E-mail: MQM-Hotline@leica-microsystems.com

2

Leica DM4000 B

Leica DM4000 M

Leica DM5000 B

Operating Manual

3

Copyrights

Copyrights

All rights to this documentation are held by Leica Microsystems Wetzlar GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other methods (including electronic systems) is not allowed without express written permission from Leica Microsystems Wetzlar GmbH.

The term "Windows" can be used in the following text without further identification. It is a registered trademark of the Microsoft Corporation. Otherwise, no inference with regard to the free usability of product names may be drawn from the use of those names.

The instructions contained in the following documentation reflect state-of-the-art techno-logy and knowledge standards. We have compiled the texts and illustrations as accurately as possible. Nevertheless, no liability of any kind may be assumed for the accuracy of this manual’s contents. Still, we are always grateful for comments and suggestions regarding potential mistakes within this documentation.

The information in this manual is subject to modification at any time and without notification.

4

 

 

 

 

 

 

 

Contents

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Contents

 

 

 

 

 

 

 

1.

Important Notes about this Manual .....

7

6.

Startup ........................................................

31

 

 

 

6.1

Functional Principle ..................................

31

2.

Safety Notes ..............................................

8

6.2

Switching on the Microscope ................

34

2.1.

General Safety Notes ...............................

8

6.3

The Display

 

2.2.

Electrical Safety ........................................

8

 

(Leica DM4000 B/DM4000 M) .................

35

 

 

 

6.4

The Function Keys ....................................

36

3.

Overview of the Instrument ....................

10

6.5

Köhler Illumination ....................................

37

 

 

 

6.6.

Checking Phase Contrast Rings .............

39

4.

Unpacking the Microscope ....................

14

6.7

Adjusting the Light Sources ....................

40

5.

Assembling the Microscope ..................

16

7.

Operation ...................................................

46

5.1

Stage ...........................................................

17

7.1

Switching on the Microscope ................

46

5.2

Condenser ..................................................

18

7.2

Stages and Specimen Displacement ....

46

5.3

Tube and Eyepieces .................................

19

7.3

Focusing .....................................................

47

5.4

Objectives ..................................................

19

7.4

Tubes ...........................................................

 

5.5

Light Sources for the

 

48

 

 

 

 

 

 

Transmitted Light Axis .............................

20

7.5

Eyepieces ...................................................

49

5.6

Light Sources for the

 

7.6

Objectives ..................................................

50

 

Incident Light Axis ....................................

21

7.7

Magnification Changer ............................

51

5.7

Equipping the

 

7.8

Light Sources .............................................

52

 

Incident Light filter turret ........................

26

7.9

Aperture Diaphragm and

 

5.8

Polarizer and Analyzer .............................

27

 

Field Diaphragm ........................................

52

5.9

DIC Prisms ..................................................

28

 

 

 

 

 

 

5.10

Optional Accessories ...............................

29

8.

Imaging Procedure for

 

5.11 Connection to the Power Supply ............

30

 

Leica DM4000 B/Leica DM5000 B .........

53

5.12

Connection to the

 

8.1

Transmitted Light ......................................

53

 

CTR5000 Electronics Box .........................

30

 

8.1.1

Bright Field ......................................

53

 

 

 

 

8.1.2

Phase Contrast ...............................

53

 

 

 

 

8.1.3

Dark Field .........................................

54

 

 

 

 

8.1.4

Polarization .....................................

55

 

 

 

 

8.1.5

Differential

 

 

 

 

 

 

Interference Contrast ....................

56

5

Contents

8.2

Fluorescence .............................................

57

 

 

 

9.

Imaging Procedure for

 

12.

Essential

 

 

Leica DM4000 M .......................................

58

 

Wear and Spare Parts .............................

66

9.1

Incident Light .............................................

58

 

 

 

 

9.1.1

Bright Field ......................................

58

13.

Abbreviations and Pictograms ..............

67

 

9.1.2

Dark Field .........................................

58

 

 

 

 

9.1.3

Polarization .....................................

59

14.

Index ...........................................................

68

 

9.1.4

Interference Contrast ....................

60

 

 

 

9.2

Transmitted Light ......................................

60

15.

EU Declaration of Conformity ................

69

 

9.2.1

Bright Field ......................................

60

 

 

 

10.

Trouble Shooting ......................................

61

 

 

 

11.

Care of the Microscope ...........................

64

 

 

 

11.1 Dust Cover ..................................................

64

 

 

 

11.2

Cleaning ......................................................

64

 

 

 

11.3 Handling Acids and Bases ......................

65

 

 

 

6

1. Important Notes about this Manual

1. Important Notes about this Manual

Caution!

This operating manual is an essential component of the microscope, and must be read carefully before the microscope is put into operation or used.

Text symbols and their meanings:

(1.2)

p. 20

!

This operating manual contains important instructions and information for the operational safety and maintenance of the microscope and accessories. Therefore, it must be kept and taken care of.

Numbers in parentheses, such as "(1.2)", correspond to illustrations (in the example, Figure 1, Item 2).

Numbers with pointer arrows (for example

p.20), point to a certain page of this manual.

Special safety instructions are indicated with the triangle symbol shown here, and have a gray background.

Caution! The microscope and accessories can be damaged when operated incorrectly.

 

Explanatory note.

*

Item not contained in all configurations.

 

7

2. Safety Notes

2. Safety Notes

2.1 General Safety Notes

This safety class 1 device is constructed and tested in accordance with EN 61010-1/IEC 1010-1, safety regulations for electrical measuring, control, and laboratory devices.

Caution!

In order to maintain this condition and to ensure safe operation, the user must follow the instructions and warnings contained in this operating manual.

Caution!

The devices and accessories described in this operating manual have been tested for safety and potential hazards.

The responsible Leica affiliate or the main plant in Wetzlar must be consulted whenever the device is altered, modified or used in conjunction with non-Leica components that are outside of the scope of this manual.

Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims!

2.2 Electrical Safety

General specifications

Leica CTR5000 electronics box (for DM5000 B)

For indoor use only. Supply voltage: Frequency:

Power input: Fuses:

Ambient temperature: Relative humidity: Overvoltage category: Pollution degree:

Microscope

For indoor use only. Supply voltage: Frequency:

Power input: DM4000 DM5000

Fuses:

DM4000

DM5000

Ambient temperature: Relative humidity: Overvoltage category: Pollution degree:

90-250 V~

50-60 Hz max. 290 VA T6,3 A

(IEC 60127-2/3) 15-35°C

max. 80% to 30°C II

2

90-250 V~

50-60 Hz

max. 180 VA max. 290 VA

T6,3 A

(IEC 60127-2/3) See CTR5000 15-35°C

max. 80% to 30°C II

2

8

2. Safety Notes

Supply unit ebq 100

 

For indoor use only.

 

Supply voltage:

90-250 V~

Frequency:

50-60 Hz

Power input:

max. 155 VA

Fuses:

2xT2A (IEC 127)

Ambient temperature:

15-35°C

Relative humidity:

max. 80% to 30°C

Overvoltage category:

II

Pollution degree:

2

(see enclosed manual)

 

Caution!

The power plug may only be plugged into an outlet equipped with a grounding contact.

Do not interfere with the grounding function by using an extension cord without a ground wire. Any interruption of the ground wire inside or outside of the device, or release of the ground wire connection, can cause the device to become hazardous. Intentional ground interruption is not permitted!

Caution!

Through connection to the grounding connection, ancillary equipment with its own and/or extra power supply may be brought to the same ground wire potential. For connections without a ground connector, Leica Service must be consulted.

Caution!

Never use any fuses as replacements other than those of the types and the current ratings listed here. Using patched fuses or bridging the fuse holder is not permitted.

Caution!

The microscope’s electrical accessory components are not protected against water. Water can cause electric shock.

Caution!

Protect the microscope from excessive temperature fluctuations. Such fluctuations can lead to the accumulation of condensation, which can damage the electrical and optical components.

Ambient temperature: 15-35°C.

Caution!

Before exchanging the fuses or lamps, be absolutely certain to switch-off the main power switch and remove the power cable.

9

3. Overview of the Instrument

3. Overview of the Instrument

Specification

Leica DM4000 B / DM5000 B

 

Leica DM4000 M

 

 

 

 

 

 

 

Imaging Procedure

transmitted light: BF, DF, PH,

 

transmitted

BF, DF, PH

 

 

Pol (DM5000 B also ICT)

 

 

light:

ICT, Pol

 

incident light:

fluorescence

 

incident light: BF, DF, ICR, Pol

 

 

 

 

 

 

Transmitted Light Axis

 

automatic Illumination Manager

 

 

 

 

(motorized aperture diaphragm and field diaphragm,

 

 

 

motorized intensity control)

 

 

 

 

 

• automatic Constant Color Intensity Control (CCIC)

 

 

motorized shutter

 

 

 

 

 

 

 

 

Incident Light Axis

integrated into the stand

 

• integrated into the stand

 

• motorized 5x filter turret

 

• motorized 4x filter turret

 

 

(DM5000 B 8x optional)

 

automatic Illumination

 

with

FIM

(Fluorescence

 

 

Manager

 

 

 

Intensity Managemer) for de-

 

motorized shutter

 

 

creasing light intensity in 5

 

 

 

 

 

 

stages

 

 

 

 

 

 

 

mechanical “Booster Lens”

 

 

 

 

 

 

for increasing fluorescence

 

 

 

 

 

 

intensity

 

 

 

 

 

 

motorized shutter

 

 

 

 

 

 

 

 

 

 

 

 

 

Z Pinion

 

manual

 

 

 

 

 

 

 

 

 

 

 

 

 

Objective nosepiece

manual

 

 

manual

 

 

absolute coded

 

 

absolute encoded

 

• 6x with M25 thread

 

• 6x with M32 thread

 

 

(DM5000 B: 7x; mot. DIC

 

• slot for DIC prisms

 

 

objective prism turret with 4

 

 

and Pol compensators

 

 

positions optional)

 

 

(optional)

 

 

 

 

 

 

 

 

 

X/Y Stage

manual

 

 

manual

 

 

replaceable specimen stage

 

replaceable specimen stage

 

• coaxial pinion length: 155 mm

 

• coaxial pinion length: 140 mm

 

 

 

 

 

 

 

 

Tube

 

manual or motorized

 

 

 

 

 

• optionally with two camera outputs

 

 

 

 

 

 

 

 

 

 

10

3. Overview of the Instrument

Specification

Leica DM4000 B / DM5000 B

Leica DM4000 M

 

 

 

 

 

 

Condenser

 

motorized condenser head

 

 

 

• motorized condenser turret for light rings,

 

 

 

DF stop, DIC prisms

 

 

 

 

• optional polarizer integrated and motorized

 

 

automatic Köhler Illumination

 

 

 

 

 

 

 

Magnification Changer

manual

 

 

manual

 

absolute coded

absolute coded

 

1x; 1.25x; 1.6x

1x; 1.5x; 2x

 

 

 

 

 

Control Panels

 

operating buttons for all motorized microscope functions

 

 

• additional variable function keys

 

 

focusing knobs

 

 

 

 

LC display

 

 

 

 

• DM5000 B with LeicaScreen (touchscreen)

 

 

 

 

 

 

Computer Interface

 

RS232C

 

 

 

 

 

 

 

Software Tools

 

Leica DMControl for WindowsTM 2000, XP, NT;

 

 

with plugins for:

 

 

 

 

 

customitsation

 

 

 

 

 

DM Operation

 

 

 

 

 

 

(remote control)

 

 

 

 

 

basic Image Viewer

 

 

 

 

 

 

CTR5000

For Leica DM5000 B only:

 

 

Electronics Box

Separate

control unit with

 

 

 

power supply for 100W halogen

 

 

 

lamp

 

 

 

 

 

see p. 8 (electrical safety)

 

 

 

 

 

 

 

 

 

11

3. Overview of the Instrument

1

2

3

14

4

5

6

7

13 12 11 10 9 8

Fig. 1 Leica DM4000 M left side of the stand with AET22 advanced ergotube

1

Eyepiece

8

Function keys field diaphragm

2

Eyepiece tube

9

Transmitted light/incident light switch

3

Tube

10

Function keys aperture diaphragm

4

Objective nosepiece with objectives

11

Function keys: Light intensity

5

Specimen stage with specimen holder

12

Focus dial with coarse and fine adjustment

6

Condenser

13

Variable function keys (factory pre-assigned)

7

LC display

14

Lamp adjustment window

12

3. Overview of the Instrument

15

22

16

21

20

19 18

17

Fig. 2 Leica DM4000 B right side of the stand with Advanced Ergotube AET22

15Lamp housing for incident light

16Lamp housing for transmitted light

17Transmitted light filter, optional

18Transmitted light filter, optional

19Variable function keys (factory pre-assigned)

20X/Y coaxial drive, height adjustable

21Focus fine adjustment

22Motorized filter cube exchanger

13

4. Unpacking the Microscope

4. Unpacking the Microscope

The device is delivered in two boxes.

The stand box contains the following components:

Stand with integrated incident light axis and objective nosepiece

Specimen stage with stage bracket

Power cable and PC connecting cable

CD with Leica software package

Instructions and list of microscope default settings (“Identification Sheet”)

The system box contains the microscope accessories:

Tube

Eyepieces

Objectives

Condenser

Lamp housings with accessories

Fitting tool

Depending on configuration, additional microscope accessories such as filter cubes, etc.

The external ebq 100 supply unit* is delivered in separate packaging.

For the Leica DM5000 B microscope:

The CTR5000 electronics box is also delivered in separate packaging.

First, carefully remove all components from the transportation and packaging materials.

Note:

Avoid touching the lens surfaces of the objectives. If fingerprints do appear on the glass surfaces, remove them with a soft leather or linen cloth. Even small traces of finger perspiration can damage the surfaces of optical surfaces in a short time. See the chapter, "Care of the microscope" → p. 64, for additional instructions.

Caution!

Do not yet connect the microscope and peripherals to the power supply at this point!

14

4. Unpacking the Microscope

Installation location

Work with the microscope should be performed in a dust-free room, which is free of oil vapors and other chemical vapors, as well as extreme humidity. At the workplace, large temperature fluctuations, direct sunlight and vibrations should be avoided. These conditions can distort measurements and micrographic images.

Allowable ambient conditions

Temperature

15-35°C

Relative humidity

maximum 80% up to 30°C

Microscopes in warm and warm-damp climatic zones require special care in order to prevent the build up of fungus.

See the chapter, "Care of the microscope" → p. 64, for additional instructions.

Caution:

Electrical components must be assembled at least 10 cm from the wall and away from flammable substances.

Transport

For shipping or transporting the microscope and its accessory components, the original packaging should be used.

As a precaution to prevent damage from vibrations, the following components should be disassembled and packaged separately:

Unscrew the objectives.

Remove the condenser.

Remove the stage.

Remove the lamp housings.

Disassemble the burner of 106 z lamp housing.

Remove all moving or loose parts.

15

5. Assembly

5. Assembling the Microscope

The microscope components are logically assembled in this order:

Stage

Condenser

Tube

Eyepieces

Objectives

Light sources

Filter cubes/reflectors*

Only a few commonly used screwdrivers and keys are necessary for assembly, which are included in the delivery package.

When using intermediate systems and optical accessories, the sequence may vary.

In this case, read Chapter,

"5.10 Optional accessories" → p. 29

16

5. Assembly

5.1Stage

!Caution:

Before assembling the stage, make sure no objectives are installed!

Place the specimen holder on the stage and fasten it with the two screws (3.1).

From above, set the stage clamp onto the dovetail guide (4.2) and push the stage downwards until the upper end of the dovetail guide is tightly fastened to the upper end of the stage clamp.

Firmly tighten the stage clamp (4.1).

Using the condenser height adjuster (3.2), turn the condenser holder completely upwards, i.e. as close to the stage as possible.

Loosen the stage clamp (3.3) slightly.

Note:

For thicker specimens (Leica DM4000 M) the stage can be set to a correspondingly lower level.

Fig. 3

Mechanical object stage

Fig. 4

Assembling the stage

1

Locking screws for specimen holder

1

Stage clamp

2

Condenser height adjuster

2

Dovetail guide

3

Stage clamp

 

 

 

1

1

2

2 3

17

5. Assembly

5.2 Condenser

Using the condenser height adjuster (5.4), turn the condenser holder (5.1) completely downwards.

Unscrew the clamping screw for the condenser (5.3) far enough so that the condenser can be inserted from the front.

From the front, insert the condenser into the condenser holder as far as it will go. On the underside of the condenser, there is an orientation pin (6.1), which must be located in the guiding notch (7.1).

Pull the condenser’s clamping screw (5.3) so that the condenser is locked in place.

Connect the condenser over the connection (8.1) with the stand.

Fig. 5 Condenser holder

1Condenser holder

2Condenser centering

3Clamping screw for condenser

4Condenser height adjuster

Note:

The condenser must be centered before using the microscope.

→ Köhler illumination p. 37.

Fig. 6

 

Underside of condenser

1

1 Orientation pin

Fig. 7 Condenser holder 1 Guiding notch

1

Fig. 8 Condenser connector 1 Condenser cable socket

1

 

 

 

 

 

 

 

 

 

 

 

 

 

 

2

3

4

 

 

1

 

 

 

 

 

18

5. Assembly

5.3 Tube and Eyepieces

The tube is mounted to the stand either directly or with the use of intermediate modules. It is fastened in place with the side clamping screw (9.1).

Loosen the clamping screw (9.1).

Insert the tube in the circular receptacle (dovetail ring).

Retighten the clamping screw (9.1).

Only for the MBDT motorized tube:

Connect the tube to the stand with the connector socket (10.1).

The eyepieces are inserted into the eyepiece tubes on the tube.

6.4 Objectives

The receptacles on the objective turrets are numbered (Fig. 11). The individual objectives have already pre-assigned positions at the factory according to their configuration.

A list of the exact objective positions is provided in shipment. (“Identification Sheet”)

!Caution:

Cover unoccupied threads on the turret with dust protector caps!

Fig. 9 Fastening the tube 1 Clamping screw

1

Fig. 10 Motorized tube connection 1 Connector socket

1

Fig. 11

Objective turret with labeled objective receptacles

19

5. Assembly

5.5 Light Sources for the Transmitted Light Axis

Fig. 12

 

Lamp housing 107/2

 

Releasing the

Caution:

fastening screw

Be sure that the lamp housing is disconnected from the power supply. Unplug the power plug and the power supply during assembly.

107/2 Lamp Housing

This lamp housing is used with a 12V 100W halogen lamp, which is already mounted.

In case the lamp has to be removed:

Remove the fastener screw on the housing (Fig. 12).

Remove the housing by pulling it upwards.

Remove the lamp

Insert the new 12V 100W lamp (13.1) with dust cover straight into the socket until it stops. Be sure that the lamp is inserted straight.

Remove the lamp’s dust cover.

Fig. 13

1

Lamp housing 107/2,

opened

 

1

Mount with

 

 

halogen lamp

 

2

Collector

 

 

 

2

Caution:

Do not remove the lamp’s dust cover until you have installed the lamp. Avoid fingerprints on the lamp.

Replace the housing and fasten it in place using the fastening screw.

Place the lamp housing in the transmitted light lamp housing receptacle (14.2) and fasten it with the clamping screw on the side.

Connect the lamp housing to the power supplyfor transmitted light (symbol: ) (14.3).

Fig. 14 Rear side of stand

1Incident light lamp housing receptacle

2Transmitted light lamp housing receptacle

312 V 100 W connection for transmitted light (symbol: )

4 12 V 100 W connection for incident light (symbol:

)

1

2

3 4

 

20

5. Assembly

5.6 Light Sources for the Incident Light Axis

Caution:

During assembly, always unplug the power supply unit of the 106 z lamp housing from its socket.

Never touch the glass parts of the burner with bare hands.

Never look directly into the beam path (blinding hazard).

During assembly work on xenon burners, always wear the supplied protective gloves and face protection (Fig. 15) (risk of explosion).

106 z lamp housing

Fig. 15

Protective gloves and mask

This lamp housing is used with a 12V 100W halogen lamp or various gas discharge lamps.

Inserting the 12V 100W halogen lamp into the 106 z lamp housing

Unscrew the fastening screws of the cover and lift up the cover (16.1).

Unscrew the fastening screws of the lamp mount (16.8) and pull out the mount (Fig. 17).

Fig. 16 106 z lamp housing (on the side, open)

1Cover raised

2Collector

312 V 100 W lamp or

gas discharge lamp in mount

4Reflector (mirror)

5, 6, 7 Adjusting screw for x-y reflector

8Fastening screw for lamp mount

9Socket for contact plug

1

2

4

5

3

6

7

8 9 8

21

+ 48 hidden pages