LEICA CM1950 User Guide

Leica CM1950

THE Disinfecting Clinical Cryostats

Leica CM1950 Platform

Protect

THE cryostats for high quality sectioning, user safety and efficient workflow

The Leica CM1950 platform is the latest innovation in a long line of distinguished
cryostats manufactured by Leica Microsystems. Leica designed and developed the
new family of cryostats based upon users’ feedback and their requirements for
high quality sections, user safety, and efficient laboratory workflow.

High Quality Sectioning

High precision microtome with stepper motor object feed

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Motorized sectioning (optional)

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New stable specimen clamp system

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New flat specimen discs for high specimen stability

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New rapid-freezing shelf design

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New effective object cooling system (optional)

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User Safety

UVC disinfection

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AgProtect™ antimicrobial nanosilver surface coating

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New disposable blade holder CE

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Debris-free, frost-free enclosed microtome assembly

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Leica’s Section Waste Removal System (optional)

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Spacious cabinet with rounded edges inside and out

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Efficient Workflow

UVC disinfection at any time and temperature

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Organized work space inside and out

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Frost-free, encapsulated microtome assembly

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Leica’s Section Waste Removal System with easily-accessible,

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concealed filter system (optional)

Easily recognizable control symbols

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Visible operating parameters at all times

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The brightl
cabinet is
allow effici
ple specim

Learn quickly and work confi­dently using the externally loca­ted, self-explanatory controls
with easily recognizable sym­bols.

For an extra measure of safety, the inside of
the instrument features an ozone-free UVC
disinfection system that is certified to be
effective against most bacteria, virus, and fun­gi, even at cold temperatures.

Increased safety for the user and others in the
immediate work area: Leica’s AgProtect™
antimicrobial nanosilver surface coating
deters the propagation of infectious agents on
the outside of the instrument.

To protect the laboratory environment from
contamination, a multi-filter system (including
HEPA filter) removes particulates and cleans
the air exhausted from the optional Section
Waste Removal System

Liquid condensate
collected in an eas
ble waste container

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Store tools, glass slides, stai­ning containers, etc. in a
recessed area on top of the
cryostat to have these items
immediately at hand.

For extra ease of use and con­sistent sectioning of all speci­men types, an optional sectio­ning motor drive is available.

Leica Design by Werner Hölbl

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THE cryostats

for high quality sectioning

Obtain high quality sections through:

NEW: CryoZone™ blade holder and anti-roll guide cooling

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Maintaining the ideal object temperature for specific specimens

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Great stability of the microtome and blade holder system

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Extreme accuracy of the stepper motor object feed

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Consistent, motor-driven cutting stroke (optional)

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Vacuum-assisted sectioning aid (optional)

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High precision microtome with exact stepper motor object feed.

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CryoZone™ – the cool way to improve section quality

The CryoZone™ system controls a zone of uniformly cool air in the critical areas of the cryochamber. This
cool air circulates around the specimen, knife and anti-roll guide to create the optimal conditions for con­sistent, high-quality sectioning.

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Efficient specimen loading is achieved via single lever
operation that provides maximum clamping force indepen­dent of individual user strength.Direct contact cooling
through the (optional) actively cooled object head facili­tates rapid changes of specimen temperatures to provide
the best conditions for sectioning various specimen types.

High specimen stability is achieved by adherence to deeply
grooved specimen discs. Specimens freeze quickly
because the pre-cooled discs feature a large back surface
that fully contacts the freezing shelf with integrated Peltier
element.

Superior sectioning of routine, hard, and/or large samples is
achieved using a choice of knife/blade holders as well as the
new dove tail mounting system that provides increased sta­bility and extended working distance. The new CE holder for
high and low profile disposable blades evenly distributes
clamping pressure across the entire length of the blade,
which provides vibration-free sectioning.

The new CN and CNZ knife holders are available for steel
or tungsten carbide knives (optional accessories).

The optional vacuum sectioning aid achieves time-sav­ing section preparation and reduces section curling.

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THE cryostats

Protect

for the highest user safety

Histology laboratorires continue to express major concerns
about biohazard exposure and non-ergonomic work practices.
As a result of their feedback, the Leica CM1950 family of
cryostats is designed to provide maximum protection from these
risks at all stages of the sectioning process.

Certified UVC disinfection of exposed surfaces and air

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within the cryochamber at cold temperatures
AgProtect™ antimicrobial nanosilver coating on the

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outside surfaces of the cryostat
Optional Section Waste Removal System with a series of

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three filters
Ergonomic exterior cabinet design with rounded edges

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and comfortable working height
Optional ergonomic chair and foot rest

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Leica’s built-in UVC disinfection system provides
certified protection from infectious material and
minimizes the risk of contamination in the cry­ochamber. Scientific studies prove that surface

disinfection from bacteria, viruses, and fungi can
be achieved with UVC light. Certification proving the effectiveness
of UVC disinfection against a range of microorganisms can be
downloaded from www.leica-microsystems.com/cm1950_safety,
which includes detailed information on the test procedure and the
efficacy of UVC disinfection.

To efficiently reduce the propagation of infectious agents on
the outside surfaces of the cryostat, the Leica CM1950 family
features an antimicrobial nanosilver surface coating, called

AgProtect™. Silver (Ag) ions are well document­ed for their ability to reduce bacterial growth.

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Section waste is easily removed by using Leica’s optional
Section Waste Removal System during trimming with the
magnetized nozzle, or during extensive cleaning with the
flexible hose. Solid waste is collected in a concealed prima­ry filter system and air is filtered through a HEPA filter for
added safety in the laboratory environment. Liquid conden­sate is collected in a condensate bottle for safer disposal.

The ergonomic design of the Leica CM1950 protects the user
from sharp edges and helps prevent work-related muscu­loskeletal disorders (MSD) and repetitive strain injury (RSI).
Rounded edges allow the user to adopt a comfortable work­ing position. Bright, glare-free illumination helps reduce eye
fatigue.

The new disposable blade holder CE with integrated, colored
safety guard and blade removal aid makes blade handling
safer. For users who prefer using the brush technique during
sectioning, the palm rest can be used instead of the anti-roll
guide.

For individual user comfort, the Leica chair and height
adjustable foot rest are available as optional accessories.

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THE cryostats

for highly efficient workflow

The best workflow for efficient operation is achieved by:

Simple specimen identification using the optional, color-coded specimen discs

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Fast, efficient specimen orientation with clearly defined zero position

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Disposable blade holder for both high and low profile blades

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Frost-free, enclosed microtome assembly

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Optional motor drive for specimen sectioning

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Certified UVC disinfection at low temperatures – does not produce condensation or

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contaminated waste, is effective at low temperatures, and can be terminated at any
time to process urgent cases

Easy for the multi users: the self­explanatory, single-function keys and
easily readable LEDs help prevent
operating errors, facilitate cryosec­tioning, and make scrolling through
multiple menus unnecessary. The
control panels are comfortably posi­tioned in the armrests to eliminate
the need to reach into the cold cham­ber to use or adjust settings.

Tools, glass slides, staining contain­ers, etc. can be stored in a recessed
area on top of the cryostat to keep
them immediately at hand.

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Sectioning tools/aids etc. can be conveniently placed on
the optional, movable shelf.

The specimen discs can be pre-cooled in the optional
internal storage container, and then easily organized and
transported.

Optional color coded specimen discs help identify speci­mens coming from multiple sources. The specimen disc
features a visual specimen orientation aid to enable pre­cise orientation.

A clearly defined zero-position allows faster
specimen alignment prior to sectioning and
easy realignment if the specimen is removed
from the object head.

With the new disposable blade holder CE blade
exchange is simple and fast. The holder’s lateral
displacement function allows the use of the full
length of the blade. Small and large sections are
easily lifted from the large, horizontal pressure
plate.

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Leica CM1950 Technical Specifications

The Leica CM1950 basic instrument is equipped with CryoZone™ technology, UVC Disinfection, specimen orientation and AgProtect™
antimicrobial nanosilver coating.
An individual, tailor-made instrument can be configured by use of the various options available:

• Actively cooled object head (double compressor)

• Vacuum Section Waste Removal System

• Motorized Sectioning

• A wide range of accessories

UVC surface disinfection: 30 or 180 minutes, user selectable

Microtome

Section thickness range: Setting values: 1 to 100 µm

1.0 µm – 5.0 µm in 0.5 µm steps

5.0 µm – 20.0 µm in 1.0 µm steps

20.0 µm – 60.0 µm in 5.0 µm steps

60.0 µm – 100.0 µm in 10.0 µm steps

Trimming range: Clinical: 10 – 40 µm

Setting values: 10 µm, 20 µm, 30 µm, 40 µm
Research: 1 – 600 µm
Setting values: 1,0 µm – 10,0 µm in 1,0 µm steps

10,0 µm – 20,0 µm in 2,0 µm steps
20,0 µm – 50,0 µm in 5,0 µm steps
50,0 µm – 100,0 µm in 10,0 µm steps

100,0 µm – 600,0 µm in 50,0 µm steps
Maximum specimen size: 50 x 80 mm
Total specimen feed: 25 mm
Vertical specimen stroke: 59 mm
Specimen retraction: 20 µm or off
Specimen orientation: 8° (x-, y-axis), 360° rotation of specimen disc

Electric coarse feed: Slow: 300 µm/s, in 20 µm steps

Fast: 900 µm/s

Cryochamber

Temperature range: 0°C to -35°C at ambient temperature of 20 °C

Specimen cooling (optional)

Temperature range: – 10 to – 50 °C

Defrosting of specimen head: manual defrost

Cryochamber defrosting

Automatic cryochamber defrosting: programmable, (hot gas defrost),

selectable time,

1 defrost in 24 h or manual hot gas defrost,

defrost time: 12 minutes
Automatic shut off defrost: at – 5 °C chamber temperature

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Quick-freeze shelf

Minimum temperature: down to – 42 °C, at chamber temp. – 35°C
Number of freezing stations: 15 + 2
Defrost: manual hot-gas defrost

Peltier element

Number of freezing stations: 2
Maximum temperature difference: 17 K, at chamber temp. of – 35 °C

Power draw 1900 VA

General information

Operating temperature range: 18°C to 35°C
Relative humidity: max. 60%, non-condensing
Storage humidity: < 60%

Dimensions and Weights

Width (without handwheel): 700 mm
Width (with handwheel): 835 mm
Depth (cabinet only): 850 mm
Height (total): 1215 mm
Working height (armrest): 1025 mm

Weight: 165 kg
Weight (with vacuum): 183 kg
Weight (with motor): 185 kg
Weight (with motor and vacuum): 193 kg

Choice of blade or knife holders:

Stable blade holder base with segment arc for lateral displacement of the blade.
Disposable blade holder insert CE for high and low profile blades with 2 anti-roll guides with 50 and 100 µm spacer with glare
minimizing glass insert, colored safety guard and blade removal help. Palm rest for brush technique.

Stable knife holder base for knife holder CN or knife holder CNZ. Knife holder CN for reusable steel knives
Knife holder CNZ for reusable steel or tungsten carbide knives with central pressure plate for full usage of the knife.

Wide range of accessories available on request.
Technical specifications subject to change without prior notice.

As confirmed by the successful c_CSA_us certification, the Leica CM1950
has been designed and manufactured in compliance with US, Canadian and IEC
requirements.

State-of-the-art development, manufacturing and quality control procedures –
certified under DIN EN ISO 9001 – ensure highest quality and reliability.

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“With the user, for the user”
Leica Microsystems

Leica Microsystems operates internationally in four divi­sions, where we rank with the market leaders.

Life Science Division

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The Leica Microsystems Life Science Division supports the
imaging needs of the scientific community with advanced
innovation and technical expertise for the visualization,
measurement, and analysis of microstructures. Our strong
focus on understanding scientific applications puts Leica
Microsystems’ customers at the leading edge of science.

Industry Division

•

The Leica Microsystems Industry Division’s focus is to
support customers’ pursuit of the highest quality end result.
Leica Microsystems provide the best and most innovative
imaging systems to see, measure, and analyze the micro­structures in routine and research industrial applications,
materials science, quality control, forensic science inves­tigation, and educational applications.

Biosystems Division

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The Leica Microsystems Biosystems Division brings his­topathology labs and researchers the highest-quality,
most comprehensive product range. From patient to pa­thologist, the range includes the ideal product for each
histology step and high-productivity workflow solutions
for the entire lab. With complete histology systems fea­turing innovative automation and Novocastra™ reagents,
Leica Microsystems creates better patient care through
rapid turnaround, diagnostic confidence, and close cus­tomer collaboration.

Surgical Division

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The Leica Microsystems Surgical Division’s focus is to
partner with and support surgeons and their care of pa­tients with the highest-quality, most innovative surgical
microscope technology today and into the future.

The statement by Ernst Leitz in 1907, “with the user, for the user,” describes the fruitful collaboration
with end users and driving force of innovation at Leica Microsystems. We have developed five
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and representatives in more than 100 countries

Italian 95.7830 Rev B

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Spanish 95.7829 Rev B

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French 95.7828 Rev B

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German 95.7827 Rev B

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© Leica Microsystems GmbH • HRB 5187 • 09/2008

Order nos. of the editions in: English 95.7826 Rev B

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