Leica CM1950 User Manual
Operating Manual
Leica CM1950, V 1.1 English – 05/2007
Always keep this manual near the instrument.
Read carefully prior to working with the instrument!
Leica CM1950
Cryostat
1. NOTE
The information, numerical data, notes and value
judgments contained in this manual represent the
current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are
under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to
provide our customers with additional copies,
updates etc. of this manual.
For erroneous statements, drawings, technical
illustrations etc. contained in this manual we exclude liability as far as permissible according to
the national legal system applicable in each individual case. In particular, no liability whatsoever
is accepted for any financial loss or consequential damage caused by or related to compliance
with statements or other information in this
manual.
Statements, drawings, illustrations and other
information as regards contents or technical
details of the present manual are not to be
considered as warranted characteristics of our
products.
These are determined only by the contract provisions agreed between ourselves and our
customers.
Leica reserves the right to change technical specifications as well as manufacturing processes
without prior notice. Only in this way is it possible
to continuously improve the technology and manufacturing techniques used in our products.
This document is protected under copyright laws.
All copyrights to this documentation are held by
Leica Biosystems Nussloch GmbH.
Any reproduction of text and illustrations (or of
any parts thereof) by means of print, photocopy,
microfiche, web cam or other methods – including any electronic systems and media – requires
express prior permission in writing by
Leica Biosystems Nussloch GmbH.
For the instrument serial number and year of manufacture, please refer to the name plate at the
back of the instrument.
© Leica Biosystems Nussloch GmbH
Published by:
Leica Biosystems Nussloch GmbH
Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 (62 24) 143-0
Fax: +49 (62 24) 143-200
eMail: histo_info@leica-microsystems.com
Internet: http://www.histo-solutions.com
Leica CM1950 – Cryostat
3
Table of contents
1. Important Information ......................................................................................................................... 6
1.1 Symbols Used in This Manual and Their Meaning ................................................................ 6
1.2 Qualification of Personnel.......................................................................................................... 6
1.3 Designated Use............................................................................................................................ 6
1.4 Instrument Type ........................................................................................................................... 6
2. Safety and Design ................................................................................................................................ 7
2.1 General Safety Instructions ....................................................................................................... 7
2.2 Unpacking and Installation ........................................................................................................ 7
2.3 Safety Features .......................................................................................................................... 10
2.4 Securing/Locking the Handwheel .......................................................................................... 11
2.5 Cleaning, Disinfection – Turning the Instrument Back On.................................................. 12
2.6 Handling Specimens – Defrosting .......................................................................................... 13
2.7 Removal of the Microtome....................................................................................................... 13
2.8 Maintenance .............................................................................................................................. 13
3. Technical Data ................................................................................................................................... 14
4. Standard Delivery .............................................................................................................................. 16
5. General Overview .............................................................................................................................. 18
5.1 Control Panel Fields and Cryostat Chamber.......................................................................... 19
6. Installation ........................................................................................................................................... 20
6.1 Site Requirements ..................................................................................................................... 20
6.2 Transport to the Site ................................................................................................................. 21
6.3 Assembling the Handwheel, Centering the Handwheel (Optional) ................................... 22
6.3.1 Locking/Unlocking the Handwheel......................................................................................... 23
6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor) ................................ 23
6.4 Connection to Power Supply System ..................................................................................... 24
6.5 Installing Accessories/Inserting Chamber Accessories .................................................... 25
6.5.1 Installing the Adjustable Footrest (Optional) ........................................................................ 25
6.5.2 Installing the Storage Systems (Optional) ............................................................................. 26
6.5.4 Inserting the Section Waste Tray ...........................................................................................26
6.5.3 Shelf, Movable (Optional)......................................................................................................... 26
6.5.5 Installing the Heat Conductor Block, Stationary (Optional) ............................................... 27
6.5.6 Installing the Knife/Blade Holder and Adjusting the Clearance Angle............................. 27
4
Operating Manual V 1.1 – 05/2007
7. Instrument Controls ........................................................................................................................... 30
7.1 Control Panel Fields on the CM1950 – Control Panel Field 1 .............................................. 30
Control Panel Field 2 – Electric Coarse Feed, Sectioning
and Trimming Thickness Display ............................................................................................ 32
Control panel 3 – Motorized sectioning (optional) ............................................................... 34
8. Working with the Instrument ......................................................................................................... 36
8.1 Preparing Cutting Tools, Specimen Discs and Preparation Aids ...................................... 36
8.2 Turning the Instrument On ....................................................................................................... 36
8.3 Configuring the Parameters..................................................................................................... 37
8.4 Working with the Precooled Cryostat .................................................................................... 42
8.4.1 Preparatory Work ...................................................................................................................... 42
8.4.2 Trimming with Extraction – Anti-roll Guide Installed ........................................................... 43
8.4.3 Cutting with Extraction – Anti-roll Guide Installed ............................................................... 45
9. Troubleshooting ................................................................................................................................. 48
9.1 Problems During Work ............................................................................................................. 48
10. Temperature Selection Chart (in minus °C) .............................................................................. 52
11. Optional Accessories ....................................................................................................................... 53
11.1 Ordering Information ................................................................................................................. 53
12. Maintenance and Cleaning ............................................................................................................ 66
12.1 General Maintenance ............................................................................................................... 66
12.2 Changing Fuses .......................................................................................................................... 67
12.3 Replacement of the Lamp/UVC Lamp ..................................................................................... 68
13. Decontamination Certificate (master) ........................................................................................ 70
14. EC Declaration of Conformity ........................................................................................................ 72
15. Warranty and Service ...................................................................................................................... 73
16. Peoles Republic of China ................................................................................................................ 74
Leica CM1950 – Cryostat
5
1. Important Information
1.1 Symbols Used in This Manual and
Their Meaning
Warnings
appear in a gray box and are marked
by a warning triangle.
Notes
i.e. important user information appear
in a gray box and are marked by an information symbol.
Caution – UVC radiation!
(5)
(Fig.5)
Figures in brackets refer to item numbers in drawings or to the drawings
themselves.
1.2 Qualification of Personnel
The Leica CM1950 may be operated by trained
laboratory personnel only.
All laboratory personnel designated to operate
the instrument must carefully read the present
instruction manual prior to starting work with the
instrument.
1.3 Designated Use
The CM1950 is a high-performance cryostat with
an encapsulated microtome and separate specimen cooling. It features a UV disinfection system,
an (optional) integrated extraction system for section waste and an (optional) motor for motorized
sectioning.
The cryostat is designed to produce frozen sections
for biological, medical and industrial applications.
The CM1950 is suitable for in-vitro diagnostic
(IVD) applications.
The instrument may only be operated within the
scope of its designated use as described above
and as per the instructions given in this manual.
Any other use of this instrument is considered
as improper operation.
1.4 Instrument Type
All information given in this instruction manual
applies only to the instrument type indicated on
the title page.
A name plate, indicating the instrument serial
number, is attached to the back of the instrument.
Despite chemical and/or UV-light disinfection, personal safety precautions
as per the applicable laboratory regulations must still be taken (i.e. safety
goggles, gloves, laboratory coat and
mask must be worn).
This type of disinfection reduces the
number of germs by at least 99.99%.
Fig. 1
6
Operating Manual V 1.1 – 05/2007
2.1 General Safety Instructions
The CM1950 is a cryostat with an encapsulated microtome and separate
specimen cooling. It is primarily used for work in the area of fast-cut diagnostics. The displays and instrument controls are easy to operate due to
their largely self-explanatory symbols. LED displays make it easy to read.
The cryochamber is made of seamlessly welded, high-quality stainless steel
that is free of difficult-to-access corners and thus easy to clean and disinfect.
This instrument has been built and tested in accordance with the following
safety regulations on electrical measuring, control, regulating and laboratory devices. In order to maintain this condition and to ensure safe operation, the operator must observe the instructions and warnings contained in
this instruction manual.
For current information about applicable standards, please refer to the
CE declaration of conformity on our Internet site:
2.2 Unpacking and Installation
• To ensure proper function of the instrument, it must be set up with a
minimum distance on all sides from walls and furniture (see ‘Site
Requirements’, p. 20)
• The instrument may only be transported in an upright or slightly inclined
position.
• To ensure a safe transport with a fork lift 3 people are required: one operating the fork lift, and the other 2 holding the instrument on either
side to prevent it from sliding down.
• Before connecting to the power supply system, please check if the local
voltage complies with the power rating specified on the name plate of
the instrument (see also ‘Technical data’)!
• Never connect the instrument to a power socket that does not have a
protective conductor terminal.
Length of power cable: up to 3.5 m extension possible: no
2. Safety and Design
www.histo-solutions.com
After transporting, wait at least 4 hours before turning the instrument on. This waiting period is
necessary to allow the compressor oil, which may have been displaced during transport, to return
into its original position. Any condensation on electrical parts that has formed due to temperature differences during transport must be allowed to dry completely. Failure to comply with this
can cause severe damage to the instrument!
Leica CM1950 – Cryostat
7
2. Safety and Design
Fig. 2
When the instrument is delivered, check the tilt indicators on the
packaging.
If the arrowhead is blue, the shipment was transported laying flat,
was tilted at too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment for
possible damage.
1
5
5
7
5
4
22
3
8
8
8
8
8
11
4
9
10
11
Opening the packaging
1. Remove the 3 screws (1) on each of the two sides of the
box (7) at the top.
9
14
17
Cover = ramp
2. Remove the 2 screws on each of the 4 hinges (2) on the
front and rear side of the box.
Do NOT remove the screws of the hinges on the top
lid!
3. Remove 1 screw (3) between the two hinges on the front
and rear side of the box.
4. Remove the 3 screws (4) at the bottom on all four sides
of the box.
5. Of the two vertical rows of screws (5), remove one on
either side (which side does not matter) entirely.
Fig. 3
8
Operating Manual V 1.1 – 05/2007
Removing the packaging
2. Safety and Design
6
6
1. Remove the cover (17) and place it on the floor with the foam parts facing
downwards. - The cover will be used as a ramp later.
2. Remove the 4 foam parts (6) by pulling them upwards.
3. Open the box (7) on its side hinges like an accordion partition and remove
it.
66
4. Remove the accessories carton (8) and operating manual on the front of
the instrument.
Fig. 4
5. Pull off the dust cover (9) by pulling upwards.
6. Take off the foam strip (10) on the bottom rear.
Ramp assembly
13
12
Fig. 5
1. After the foam strip (10) is removed, 2 screws (12) are visible on the
baseplate.
2. Swing out the cover hinges (2) at the front and rear.
3. Attach the cover (17) from the rear on the transport pallet (11).
The notches (13) of the hinges (2) must be pointing towards the instrument.
4. Move the cover towards the left so that the notches (13) of the hinges
slide beneath the heads of the screws (12).
Transport to the Installation Location
The instrument must be transported in an upright position.
15
16
The cover (17) now forms a ramp on which the instrument can now be rolled
down off the transport pallet.
2
14
Fig. 6
Leica CM1950 Cryostat
17
Do not push the device by its hood (16). Use the transport grip
2
points (15 ).
The rollers (14) must run over the cover hinges (2) at the front and
rear. Danger of tipping!
1. Carefully roll the instrument backwards from the pallet.
2. Push the instrument to the installation location on the castors (14).
9
2. Safety and Design
2.3 Safety Features
The instruction manual includes important information related to the operating
safety and maintenance of the instrument.
The Operating Manual is an important part of the product, and must be read
carefully before startup and use and must always be kept near the instrument.
If additional requirements on accident prevention and environmental
protection apply in the country of operation, this Operating Manual must be
supplemented by appropriate instructions to ensure compliance with such
requirements.
The instrument is equipped with the following safety devices: an emergency stop switch (motorized instruments only), a handwheel lock and centering system (motorized instruments only), knife guard on the blade and knife
holder, and a blade ejector.
The consistent use of these safety features and strict observation of the warnings and cautions in this manual, will safeguard the operator from accidents
and/or personal injury to a great extent.
To prevent adverse health effects from UV radiation, the UV disinfection cycle can be started only after the sliding window has been properly closed. Closing the window activates the corresponding safety
features.
Microtome knives
10
• Take care when handling microtome knives/disposable blades. The
cutting edge is extremely sharp and can cause severe injury!
• Never leave knives and dismounted knife holders with a knife/blade
mounted lying around!
• Do not place a knife on a table with the cutting edge facing upwards!
We strongly recommend using the safety gloves included with the
standard delivery!
• Never try to catch a falling knife!
• Prior to manipulating the knife and specimen or changing the specimen,
always lock the handwheel and cover the cutting edge with the knife
guard!
• Avoid contact with cold parts of the instrument as this can cause frostbite!
Operating Manual V 1.1 – 05/2007
Knife guard
Prior to manipulating the knife and specimen, changing the specimen or knife, or taking a break, always lock the handwheel and
cover the cutting edge with the knife guard.
The CE, CN and CN-Z knife holders feature knife guards; the glass anti-roll
plate of the CE knife holder also serves as a knife guard.
2.4 Securing/Locking the Handwheel
Always lock the handwheel and cover the cutting edge with the
12 o’clock
position
6 o’clock
position
1
2
Fig. 7
To lock the handwheel, press the lever (1) outward. Continue turning the
handwheel slowly until the grip is in the upper or lower position and the
handwheel is locked. Press the lever fully outward; gently rock the handwheel until the locking mechanism clicks into place noticeably.
To release the handwheel, press the lever (2) on the handwheel toward the
cryostat housing.
knife guard prior to manipulating the knife or specimen, changing
the specimen, or taking a break.
2. Safety and Design
Fig. 8
Fig. 9
Leica CM1950 – Cryostat
Centering of the handwheel (motorized instruments only)
Pull out the handwheel’s handle and position it in the middle of the
handwheel. The handle automatically engages in this position.
An important safety device on the cryostat is the centering of the
handwheel for motorized instruments.
Rotate the handwheel only if the refrigeration system is on and the
cryochamber is cold.
11
2. Safety and Design
2.5 Cleaning, Disinfection – Turning the Instrument Back On
It is not necessary to remove the microtome for disinfection.
• The instrument has been designed for UV disinfection! Spray disinfection with Leica Cryofect is also possible, thanks to the special insulation
of the microtome. (Cryofect is not available in all countries!)
Remove section waste after EVERY sectioning operation and BEFORE changing specimens. Remove the section waste using a paper towel soaked in Cryofect or an alcohol-based disinfectant) or
remove the waste using the extraction nozzle (optional). Do not
start the disinfection until after folding the section stretcher to the
side! Each new specimen is a potential source of contamination.
• When disinfecting the instrument, take appropriate protective measures
(gloves, mask, protective clothing, etc.).
• When using detergents and disinfectants please comply with the safety
precautions of the disinfectant manufacturer.
• The integrated glass anti-roll guide of the blade holder CE, CN and CN-Z
can be cleaned either with acetone or alcohol.
• Dispose of waste liquid according to the waste disposal regulations!
• Do not use external heaters for drying the cryochamber. This can cause
damage to the cooling system!
• Do not turn the instrument on before the cryochamber is completely dry!
Frost formation!
• All components removed from the cryostat must be carefully dried before returning them to the cryochamber!
• The front panel and the slit cover of the microtome must be completely
dry before turning on the instrument!
12
For more detailed information about disinfection, visit the Leica
Microsystems website at
www.histo-solutions.com
Operating Manual V 1.1 – 05/2007
2.6 Handling Specimens – Defrosting
• When working with contaminated or infected material, the general safety
guidelines for laboratories must be applied.
• Before defrosting the cryochamber remove all samples!
• Before defrosting the specimen head, remove all samples!
Never leave samples in the cryochamber! - The instrument is not
suitable for storing frozen specimens, as the refrigeration dehydrates the specimens!
The quick freeze shelf can become very hot during the defrosting
process. Do not touch it during defrosting.
2.7 Removal of the Microtome
• The microtome is encapsulated and therefore does not require removal
by the user.
2.8 Maintenance
Replacing the fuses
• Turn the instrument off and disconnect the power plug before replacing
the fuses.
• Use only the fuse types specified in chapter 3, "Technical Data." Failure
to comply with this can cause severe damage to the instrument!
2. Safety and Design
Replacing the fluorescent lamp/UVC lamp
• Turn the instrument off and disconnect the power plug before replacing
the lamps.
Leica CM1950 Cryostat
It is possible to break the UVC lamp during replacement. If this
happens, the lamp change must be completed by Technical Service. If any metallic mercury is released, handle it carefully and
dispose of it properly.
If both disinfection indicator lights are blinking alternately, the
UV tube must be replaced!
13
3. Technical Data
All specifications related to temperature are valid only up to an ambient temperature of 18°C
to 35°C and a relative humidity of no more than 60%.
Type
Nominal voltage (±10 %)
Nominal frequency
Power draw
Max. start-up current for 5 sec.
Protective class
Circuit breaker:
(MDA made by Schurter)
Pollution degree
Overvoltage installation category
Heat emission (max.)
Approval
② according to IEC-1010; UL 3101
②
-1 -2 -3
100 VAC 120 VAC 230 VAC
50/60 Hz 60 Hz 50 Hz
1900 VA 1900 VA 1900 VA
35 A eff. 35 A eff. 25 A eff.
III
15A M3 15A M3 15A M3
222
II II II
1900 J/s 1900 J/s 1900 J/s
CE/c_CSA_us CE/c_CSA_us CE/c_CSA_us
Microtome
Type: Rotary microtome,
encapsulated
Section thickness range 1 to 100 μm
Trimming range:
Clinic 10 - 40μm
Research 1 - 600 μm
③
③
Total specimen feed 25 mm + 1mm
Vertical stroke 59 mm ±0.5mm
Specimen retraction 20μm (can be deactivated)
Maximum specimen size 50 x 80 mm
Cutting speed Slow: 0-50 strokes/min
Fast: 0-85 strokes/min
Vmax: 85-90 strokes/min
Specimen orientation 8° (x, y-axis)
③ For additional details, see p. 32
Electric coarse feed
Slow 300μm/s
Fast 900 μm/s
For "Site Requirements," refer to item 6.1
(page 20).
Lamp
50/60 Hz-Version: Osram DULUX L 18 W/840
Cryostat
Dimensions:
Width (w/o handwheel) 700 mm
Width (with handwheel) 835 mm
Depth (cabinet only) 850 mm
Height (total) 1215 mm
Working height (armrest) 1025 mm
Weight:
Weight (w/motor and suct.) 193 kg
Weight (w/motor, w/o suct.) 185 kg
Weight (w/o motor, w/ suct.) 183 kg
Weight (w/o motor, w/o suct.) 175 kg
Weight (w/ specimen head cool.) 165 kg
Weight (w/o specimen head cool.) 145 kg
General information
Operating temperature range: 18 °C to 35 °C
Temperature range
during storage: +5 °C to +55°C
Relative humidity: max. 60 %,
non-condensing
Storage humidity: < 60 %
14
Operating Manual V 1.1 – 05/2007
3. Technical Data
Refrigeration system CM1950 , 50 Hz CM1950, 60 Hz
Cryochamber
Temperature range 0°C to -35°C ± 5 K, adjustable in 1K increments, 0 °C to -35°C ± 5 K, adjustable in 1K increments
Cooling time to -25°C approx. 5 h approx. 5 h
Cooling time to -35°C approx. 8 h approx. 8 h
Refrigeration capacity ① 690 W 690 W
Cut-out pressure 25 bar 25 bar
Safety factor 3 3
Refrigerant* 300 g (± 5 g) refrigerant R -404A* 300 g (± 5 g) refrigerant R -404A*
Compressor oil* 0.6 l EMKARATE RL22S, ICI* 0.6 l EMKARATE RL22S, ICI*
Defrosting of cryochamber
Automatic defrosting
Programmable: Yes (hot gas defrost) Selectable time Yes (hot gas defrost) Selectable time
Defrosting intervals 1 defrost in 24 h 1 defrost in 24 h
Defrost time: 12 minutes 12 minutes
Automatic shutoff Defrost: at -5 °C chamber temperature at -5 °C chamber temperature
Quick-freeze shelf
Minimum temperature: - 42 °C (+ 5 K), at chamber temp. -35°C - 42 °C (+ 5 K), at chamber temp. -35°C
Number of freezing stations: 15+2 15+2
Defrost Manual hot-gas defrost Manual hot-gas defrost
Peltier element
Number of freezing stations: 2 2
Max. temperature difference: 17 K, at chamber temp. of -35°C 17 K, at chamber temp. of -35°C
at ambient temperature of 20°C ambient temperature of 20°C
or manual hot gas defrost or manual hot gas defrost
Specimen cooling
Temperature range -10 to -50 °C ± 3 K -10 to -50 °C ± 3 K
Refrigeration capacity① 320 W 320 W
Cut-out pressure 25 bar 25 bar
Safety factor 3 3
Refrigerant and quantity at 230 V/50 Hz 140 g (± 5 g), refrigerant R-404A* at 120 V/60 Hz 110 g (± 5 g), refrigerant R-404A*
at 100V/50/60 Hz 110 g (± 5 g), refrigerant R-404A*
Compressor oil* 0.4 l alpha 22, Kyodo* 0.4 l alpha 22, Kyodo*
Defrosting of specimen head
Automatic defrost: No No
Manual defrost
Defrost time 15 min. 15 min.
*) Refrigerant and compressor oil must be replaced by qualified, authorized service personnel only.
① acc. to CECOMAF: liquid temperature 45 °C, evaporation temperature: -25 °C
Leica CM1950 – Cryostat
15
4. Standard Delivery
Basic instrument WITHOUT motor/WITHOUT extraction, in the specific voltage variant
1 handwheel, manual .........................................................................................0477 41346
5 specimen discs, 30 mm ...................................................................................0477 40044
1 section waste tray ...........................................................................................0477 40062
1 position holder for freeze shelf ..................................................................... 0477 40080
1 freeze shelf cover ............................................................................................0477 43763
1 tool set ............................................................................................................... 0436 43463
- 1 brush, fine ................................................................................................0183 28642
- 1 Leica brush w/magnet ........................................................................... 0183 40426
- 1 Allen key, No. 1.5 ..................................................................................... 0222 10050
- 1 Allen key, No. 2.5 ..................................................................................... 0222 04137
- 1 Allen key, No. 3.0 ..................................................................................... 0222 04138
- 1 Allen key, No. 4.0 ..................................................................................... 0222 04139
- 1 Allen key with dome head, No. 4.0 ....................................................... 0222 32131
- 1 Allen key, No. 5.0 ..................................................................................... 0222 04140
- 1 key with handle, No. 5.0 .........................................................................0194 04760
- 1 Allen key, No. 6.0 ..................................................................................... 0222 04141
- 1 Single-head wrench, No. 13 .................................................................0330 33149
- 1 Single-head wrench, No. 16 .................................................................0330 18595
1 power cable .........................................................................................................................
1 bottle of cryostat oil, type 407, 50 ml ............................................................0336 06098
1 bottle of OCT freezing compound, 125 ml ....................................................0201 08926
1 pair of safety gloves, size M, * for cryosectioning ....................................0340 29011
1 user manual plus language CD ..................................................................... 0708 37115
* Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves, size S
(0340 40859) is included.
Basic instrument WITHOUT motor and WITH extraction,
Standard scope of delivery as above,
plus:
1 accessory set (extraction) .............................................................................0477 43300
- Hose adapter 1 ...........................................................................................0477 40293
- Hose adapter 2 ...........................................................................................0477 40294
- Suction nozzle ............................................................................................ 0477 40295
- Silicone hose .............................................................................................. 0477 43302
- Silicone stopper .........................................................................................0477 43304
- Chamber suction nozzle ........................................................................... 0477 43779
- Set of filters (5 pieces) .............................................................................. 0477 43792
Compare the delivered components with the parts list and your order. Should you
find any discrepancies, please contact your Leica sales office without delay.
A choice of different knife holders is available for the CM1950.
16
Operating Manual V 1.1 – 05/2007
4. Standard Delivery
Basic instrument WITH motor/WITHOUT extraction, in the specific voltage variant
1 handwheel, motorized .................................................................................... 0477 41347
5 specimen discs, 30 mm ...................................................................................0477 40044
1 section waste tray ...........................................................................................0477 40062
1 position holder for freeze shelf ..................................................................... 0477 40080
1 freeze shelf cover ............................................................................................0477 43763
1 tool set ............................................................................................................... 0436 43463
- 1 brush, fine ................................................................................................0183 28642
- 1 Leica brush w/magnet ........................................................................... 0183 40426
- 1 Allen key, No. 1.5 ..................................................................................... 0222 10050
- 1 Allen key, No. 2.5 ..................................................................................... 0222 04137
- 1 Allen key, No. 3.0 ..................................................................................... 0222 04138
- 1 Allen key, No. 4.0 ..................................................................................... 0222 04139
- 1 Allen key with dome head, No. 4.0 ....................................................... 0222 32131
- 1 Allen key, No. 5.0 ..................................................................................... 0222 04140
- 1 key with handle, No. 5.0 .........................................................................0194 04760
- 1 Allen key, No. 6.0 ..................................................................................... 0222 04141
- 1 Single-head wrench, No. 13 ................................................................. 0330 33149
- 1 Single-head wrench, No. 16 ................................................................. 0330 18595
1 power cable .........................................................................................................................
1 bottle of cryostat oil, type 407, 50 ml ............................................................0336 06098
1 footswitch dummy ........................................................................................... 0443 30420
1 bottle of OCT freezing compound, 125 ml ....................................................0201 08926
1 pair of safety gloves, size M, * for cryosectioning ....................................0340 29011
1 operating instructions plus language CD .................................................... 0708 37115
* Note: for the Japanese version: 100V, 50/60 Hz; 1 pair of safety gloves,
size S (0340 40859) is included.
Basic instrument WITH and WITH extraction, in the specific voltage variant
Standard scope of delivery as above,
plus:
1 accessory set (extraction) .............................................................................0477 43300
- Hose adapter 1 ...........................................................................................0477 40293
- Hose adapter 2 ...........................................................................................0477 40294
- Suction nozzle ............................................................................................ 0477 40295
- Silicone hose .............................................................................................. 0477 43302
- Silicone stopper .........................................................................................0477 43304
- Chamber suction nozzle ........................................................................... 0477 43779
- Set of filters (5 pieces) .............................................................................. 0477 43792
Leica CM1950 – Cryostat
Compare the delivered components with the parts list and your order. Should you
find any discrepancies, please contact your Leica sales office without delay.
A choice of different knife holders is available for the CM1950.
17
5. General Overview
Cryostat chamber
(with activated
UV disinfection)
Bacterial filter
(optional, only for
instruments with filter)
Emergency-stop
switch (motorized
instruments only)
On/off switch, also circuit
breaker
Footswitch dummy
(motorized
instruments only)
Handwheel in
12 o’clock
position
Unscrew and align
adjusting feet after
transport
18
Catch tank for
condensate
Fig. 10
Castors for safe transport
over short distances
Align adjusting feet for secure
upright position
Operating Manual V 1.1 – 05/2007
5. General Overview
5.1 Control Panel Fields and Cryostat Chamber
1 - Control panel field 1: Extraction, temperature and time control, illumination, UV disinfection
2 - Control panel field 2: Electric coarse feed (sectioning and trimming thickness adjustment)
3 - Control panel field 3: Motorized sectioning cutting, optional (adjustment of stroke type, cutting speed etc.)
1
7
4
2
6
10a
5
10b
15
16
10
11
10c
14
3
8
9
4 - Heat conductor block, stationary (optional)
5 - Peltier element (with 2 stations)
6 - Freeze shelf, 15 positions
7 - Position holder on freeze shelf
8 - Heat and cold extractor, mobile (opt.)
9 - Shelf, movable (optional)
10 - Blade holder CE with blade ejector (a)
10b- Finger rest on the blade holder CE
Leica CM1950 – Cryostat
13
12
10c- Knife guard on the blade holder CE
11 - Extraction nozzle on the extraction hose
12 - Extraction hose for section waste
13 - Brush shelf (optional)
14 - Adapter piece for extraction hose
(the coarse filter insert is behind it)
15 - Object head, directional
16 - Waste tray
Fig. 11
19
6. Installation
6.1 Site Requirements
The place of installation must meet the following requirements:
• No direct sunlight.
• Power supply at a distance no greater than approx. 3 m.
• No drafts (air condition outlets etc.).
• Even floor.
• Vibration-free floor.
• The instrument is designed for indoor use only.
• Obstruction-free access to the handwheel.
• The power switch/circuit breaker must be freely and easily accessible.
• Room temperature always approx. 22°C.
• The relative humidity must not exceed 60%.
To ensure proper function of the instrument, it must be set up while
maintaining a minimum distance from walls and furniture.
• Distance to walls and furniture, calculated from the cabinet:
- rear: 15 cm
- right side: 30 cm
- left side: 15 cm
• No heat dissipating appliances around.
20
High room temperatures and excessive air humidity affect the
cooling capacity of the cryostat and lead to ice forming in the
instrument!
• Plug the instrument into power sockets with ground only.
Power cable length: up to 3.5 m
Extendable: no
Operating Manual V 1.1 – 05/2007
6.2 Transport to the Site
• First, check if the location meets the conditions specified in "Site
• Transport the instrument to the desired location.
• Observe the following:
requirements".
The instrument must be transported in an upright position or slightly tilted (max. 30°)!
When tilting the instrument 2 people must counterbalance from the
front side to prevent the instrument from falling down and causing
severe injury!
6. Installation
• When transporting the instrument on wheels
(2) grip the cabinet only at the marked locations (
• To do so, unscrew the adjustable feet using
the No. 13 open-end wrench (when subsequently transporting the instrument on castors, screw the feet back in as far as they will
go). To ensure a secure upright position at its
intended location, align both adjusting feet (1).
).
1
Fig. 12
Leica CM1950 – Cryostat
2
2
1
2 (covered)
Before transport or relocation, remove
the filter bag from the chamber. If you
fail to do this, the filter bag will thaw,
then freeze solid when the instrument
is reconnected. When subsequently
removed, the filter will be destroyed,
causing section waste to get into the
bacteria filter. (Refer to p. 26.)
When not using the extraction for a long
period, tightly close the opening for the
extraction hose using the silicone stopper (5, page 28) included in the scope of
delivery.
21
6. Installation
Transport with a fork lift
• The instrument can be transported with a fork lift.
• At the installation site, unscrew the adjustable feet (1) with the openend wrench No. 13 (see Fig. 12). This is absolutely necessary for a stable
stand.
Total width with handwheel
Fig. 13
6.3 Assembling the Handwheel
To ensure a safe transport with a fork lift 3 people are required:
one operating the fork lift, and the other 2 holding the instrument
on either side to prevent it from sliding down.
22
Rotate the handwheel only if the
refrigeration system is on and the
cryochamber is cold.
2
1
4
• Insert the pin (1) of the handwheel shaft into
the hole (2) of the handwheel.
• Tighten the screw (4) using the No. 6 Allen key.
• Place the protective cap on the screw (4).
Fig. 14
To dismount, proceed in reverse order.
Operating Manual V 1.1 – 05/2007
6.3.1 Locking/Unlocking the Handwheel
Only rotate the handwheel when the refrigeration system is on and
12 o’clock
position
6 o’clock
position
the cryochamber is cold!
Always lock the handwheel prior to manipulating the knife or
specimen, changing the specimen, or taking a break.
To lock the handwheel, move its handle to the 12 or 6 o’clock position. Press
Fig. 15
the lever (1) fully outward; gently rock the handwheel until the locking mechanism clicks into place noticeably.
To release the handwheel, press the lever (2) on the handwheel toward the
1
2
Fig. 16
cryostat housing.
Centering the Handwheel (Optional)
An important safety device on the cryostat is the feature for centering the handwheel in motorized sectioning mode.
6. Installation
To do so, pull out the handwheel’s handle and position it in the middle of the
handwheel. The handle automatically engages in this position.
Fig. 17
6.3.2 Installing the Footswitch Dummy (Instruments with Cutting Motor)
• The footswitch dummy must be installed on the outer right side of the
instrument (see page 16, General overview) if no footswitch (optional) is
used.
If the red LED in the E-STOP field in control panel field 3 is illuminated, either:
• emergency-stop function active, or
•
footswitch dummy (optional footswitch) connected incorrectly or not at all.
Fig. 18
Leica CM1950 Cryostat
23
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