Jenway 7315 User Manual

Spectrophotometers

Models 7310 & 7315

Operating Manual

731 005 REV E/12-10

2

Safety

Please read this information carefully prior to installing or using this equipment.

1.The unit described in this manual is designed be operated only by trained personnel. Any adjustments, maintenance and repair must be carried out as defined in this manual, by a person qualified to be aware of the hazards involved.

2.It is essential that both operating and service personnel employ a safe system of work, in addition to the detailed instructions specified in this manual.

3.Other than for those items defined in the maintenance procedures herein there are no user serviceable items in this instrument. Removal of covers and attempted adjustment or service by unqualified personnel will invalidate the warranty and may incur additional charges for repair.

4.References should always be made to the Health and Safety data supplied with any chemicals used. Generally accepted laboratory procedures for safe handling of chemicals should be employed.

5.If it is suspected that safety protection has been impaired in any way, the unit must be made inoperative and secured against any intended operation. The fault condition should immediately be reported to the appropriate servicing authority.

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Contents

		Page
Safety		3
Section 1 - Introduction		8
1.1	Instrument description	8
1.2	Instrument specification	8
Section 2 - Installation		12
2.1	Unpacking	12
2.2	Installation	12
2.3	Display	13
2.4	Controls	14
2.5	Rear panel	15
2.6	Front panel	15
Section 3 - Theory and practice of spectroscopy measurements		16
3.1	Theory of spectroscopy measurement	16
3.2	Spectroscopy measurement	16
3.3	Good practice guidelines	17
Section 4 - Instrument setup		19
4.1	Navigating and screen setup	19
4.2	Time and date	20
4.3	Instrument settings menu	20
4.4	Security and setting passwords	21
4.4.1	Setting security codes	21
4.4.2	Settings lock	21
4.4.3	Method lock	21
4.5	Mode selection	22
4.6	Diagnostics	22
4.7	GLP settings	22
4.8	Screen contrast	23
4.9	Lamp save	23
Section 5 - Photometrics		25
5.1	Mode specific parameters	25
5.2	Method set up	25
5.2.1	Selecting a wavelength	26
5.3	Calibration	26
5.4	Sample measurment	26
Section 6 - Concentration		27
6.1	Mode specific parameters	27
6.2	Method setup	27
6.2.1	Selecting a wavelength	27
6.2.2	Settings	28
6.2.2.1	Selecting concentration units	28
6.2.2.2	Changing the resolution	29
6.2.2.3	Using a standard	29

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6.2.2.4	Using a factor	29
6.3	Calibration	29
6.3.1	Calibrating to a standard	29
6.3.2	Calibrating to a factor	30
6.4	Sample measurement	30
6.4.1	Measuring a sample after calibrating to a standard	30
6.4.2	Measuring a sample after calibrating to a factor	30
Section 7 - Spectrum		31
7.1	Mode specific parameters	31
7.2	Method setup	32
7.2.1	Scan settings	32
7.2.1.1	Selecting absorbance or % transmittance	32
7.2.1.2	Setting start and end wavelengths	32
7.2.1.3	Setting the scan interval	33
7.2.1.4	Y-axis scaling	34
7.3	Calibration	34
7.4	Sample measurement	34
7.5	Data analysis	35
7.5.1	Peaks and valleys threshold	35
7.5.2	Peaks and valleys table	36
7.5.3	Spectral points analysis	36
Section 8 - Quantitation		38
8.1	Mode specific parameters	38
8.2	Method setup	39
8.2.1	Selecting a wavelength	39
8.2.2	Quantitation table	39
8.2.2.1	Selecting number of standards	39
8.2.2.2	Selecting concentration units	39
8.2.2.3	Changing the resolution	40
8.2.2.4	Selecting absorbance or % transmittance	40
8.2.2.5	Adding standards	40
8.2.3	Standard curve	40
8.2.3.1	Creating a new standard curve	41
8.3	Calibration	42
8.4	Sample measurement	42
8.5	Data analysis	43
Section 9 - Kinetics		44
9.1	Mode specific parameters	44
9.2	Method set up	45
9.2.1	Kinetics settings	45
9.2.1.1	Y-axis scaling	45
9.2.1.2	Setting lag time or start on level	46
9.2.1.3	Selecting absorbance or % transmittance	46
9.2.1.4	Changing the resolution	47
9.2.1.5	Selecting concentration units	47
9.2.1.6	Using a standard	47
9.2.1.7	Using a factor	47
9.2.1.8	Selecting a wavelength	48

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9.2.1.9	Setting the kinetics measurement time	48
9.3	Calibration	48
9.4	Sample measurement	48
9.5	Data analysis	49
Section 10 - Saving, printing and autologging		51
10.1	Saving methods	51
10.1.1	Saving methods to internal memory	52
10.1.2	Saving methods to USB memory stick	52
10.2	Opening methods	52
10.2.1	Opening methods from internal memory	52
10.2.2	Opening methods from USB memory stick	53
10.3	Deleting methods	53
10.4	Saving results	53
10.5	Opening results	54
10.6	Deleting results	55
10.7	Printing	55
10.7.1	Print setup	56
10.7.1.1	Print setup – photometrics and concentration	56
10.7.1.2	Print setup - spectrum	56
10.7.1.3	Print setup – quantitation	57
10.7.1.4	Print setup – kinetics	57
10.7.2	Printing results	57
10.8	Autologging	58
10.8.1	Setting the number of sample repetitions	58
10.8.2	Selecting result’s destination	59
10.9	Connecting to a PC	59
Section 11 - Accessories and spare parts		60
11.1	Optional accessories	60
11.2	Connecting the accessories	60
11.2.1	Internal printer	60
11.2.2	Passive accessories	61
11.2.3	Active accessories	61
11.2.3.1	Automatic 8 cell turret	62
11.2.3.2	Peltier	62
11.2.3.3	Sipper pump	63
11.2.3.4	Combined sipper peltier pump	64
11.3	Using the accessories	65
11.3.1	Automatic 8 cell turret	65
11.3.1.1	Supporting creation of a standard curve in quantitation	65
11.3.2	Peltier	66
11.3.3	Sipper pump	66
11.3.3.1	Manual sipper pump settings	66
11.3.3.2	Timed sipper pump settings	67
11.3.4	Combined sipper peltier pump	69
11.4	Spares	70
Section 12 - Maintenance and service		71
12.1	Routine maintenance	71
12.2	Lamp replacement	71
12.2.1	Tungsten halogen lamp replacement	71

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12.2.2	Xenon lamp module replacement		71
12.3	Service		72
Section 13		- Troubleshooting	73
13.1	Error codes		73
13.2	Troubleshooting guide		75
13.3	Technical support		75
Section 14		- Declaration of conformity	76
Section 15		- Glossary of icons	78
Index			85

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Section 1 - Introduction

1.1INSTRUMENT DESCRIPTION

The 7310 and 7315 spectrophotometers are suited to a wide range of applications in education, quality control, environmental and clinical analysis. The 7310 is a visible spectrophotometer covering a wavelength range from 320nm to 1000nm. The 7315 is a UV/Visible spectrophotometer with a wavelength range from 198nm to 1000nm. Both models have five measurement modes: photometrics, concentration, spectrum scanning, quantitation and kinetics. These instruments use icon driven software and have an improved navigation system for easy and intuitive usability.

1.2INSTRUMENT SPECIFICATION

	7310	7315
Wavelength
Range	320 to 1000nm	198 to 1000nm
Resolution		1nm
Accuracy		± 2nm
Repeatability		± 0.5nm
Spectral bandwidth		5nm
Photometrics
Transmittance		0 to 199.9%
Absorbance		-0.300 to 2.500A
Accuracy	±1%T, ±0.01Abs at 1.000 Absorbance
Resolution		0.1%T, 0.001A
Stray light	<0.5% at 340nm	<0.5% at 340nm and 220nm
Concentration
Range		-300 to 9999
Resolution	Selectable 1/0.1/0.01/0.001
Calibration	Blank with a single standard or factor
Units	no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM, µM, nM, U, U/l, U/ml, g/l, mg/l,
	µg/l, ng/l, g/dl, mg/dl, µg/dl, mg/ml, µg/ml, ng/ml, µg/µl, ng/µl, mol/l, mmol/l
Factor		0.001 to 10000
Standard		0.001 to 1000
Quantitation
Range		-300 to 9999
Resolution	Selectable 1/0.1/0.01/0.001
Calibration	Blank with up to 6 standards
Units	no units, %, ppm, EBC, SRM, mEq/l, mEq, M, mM, µM, nM, U, U/l, U/ml, g/l,
mg/l, µg/l, ng/l, g/dl, mg/dl, µg/dl, mg/ml, µg/ml, ng/ml, µg/µl, ng/µl, mol/l, mmol/l
Curve fit algorithms	Quadratic, quadratic through zero, linear, linear through zero, interpolate
Kinetics
Measurement Time		2 to 9999 seconds
Calibration	Blank with a single standard or factor
Display	Concentration, rate of change, initial and final absorbance/%T
Resolution	Selectable 1/0.1/0.01/0.001

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	7310	7315
Spectrum
Range	320 to 1000nm	198 to 1000nm
Scan interval	Selectable 1, 2 or 5nm
Analysis	Absorbance or % transmittance and peak and valley wavelengths
Other
Beam height	15mm
Light source	Tungsten halogen lamp	Xenon lamp
Lamp save	Yes	Not applicable
GLP	Current time and date, user ID, settings lock and method lock
Number of users	999
Methods memory	48 in each measurement mode
Results memory	Limited by attached mass storage device
Removable media	USB (supplied)
Outputs	USB, Analogue, RS232, Internal printer
Power	24V
Size (w x d x h)	275 x 400 x 220mm
Weight	6kg

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Section 2 - Installation

2.1UNpacking

Remove the 7310 or 7315 from the packaging and ensure the following items are included:

1.Model 7310 spectrophotometer (731 001), or Model 7315 spectrophotometer (731 501)

2.24V 65W power supply unit (021 060)

3.Pack of 100 disposable plastic visible wavelength cuvettes (060 084), or pack of 100 disposable UV plastic cuvettes (060 230)

4.2 GB USB memory stick (019 146)

5.Jenway 73 series PC software (735 100) and interface cable (013 203)

6.Instruction manual (731 005)

7.Jenway Foreign Manual CD (JENMANCD)

8.Optional accessories (as ordered)

2.2INSTALLATION

Models 7310 and 7315 are supplied ready to use.

The unit should be placed on a clean flat surface which is free from drafts and vibrations. The units are designed for operation on 90V to 264V AC input at 47 to 63Hz. Select the correct plug attachment and attach to the power supply unit as shown below:

Fig 2.2.1 – Power supply unit with various plugs

Connect the power supply unit to the power inlet socket on the rear panel of the instrument and connect to the mains socket. Turn the power on at the mains and switch the instrument on using the power switch on the rear of the instrument.

The instrument will perform several power on tests before displaying the main menu:

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1

2

7310

3

4

09:02

5

Fig 2.2.2 – All Power On Tests Complete

1.Instrument check – ensures the validity of the saved parameters

2.Dark test

3.Checks for the accessory fitted. If an active accessory is found the instrument verifies communication and response

4.Self calibration of wavelengths

5.Checks communication between USB memory stick port and the instrument

2.3DISPLAY

These spectrophotometers have a dot matrix display which enables icons and graphs to be displayed clearly. Following successful completion of the power on tests the main menu screen will be displayed:

	1	8
		7310
	2	7

09:02

3

4

5

6

Fig. 2.3.1 – Display

1.Spectrum measurement mode

2.Photometrics measurement mode

3.Back key

4.Time and date menu

5.Quantitation measurement mode

6.Instrument settings menu

7.Concentration measurement mode

8.Kinetics measurement mode

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2.4CONTROLS

The keypad used for these models enables an easy and effective way of navigating the different measurement modes, entering numbers, saving and analysing results. The soft keys are active when an icon is displayed above or adjacent to the key. The only exception to this is the back key which is always active.

The main menu screen and surrounding keypad is displayed below.

	1	7310	8
	1		8
	2		7
	2		7
		09:02
	3

4

5

5

5

6

Fig. 2.4.1 – Display

1.Spectrum measurement mode

2.Photometrics measurement mode

3.Back key

4.Time and date menu

5.Quantitation measurement mode

6.Instrument settings menu

7.Concentration measurement mode

8.Kinetics measurement mode

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2.5REAR PANEL

The image below shows the rear panel on the instrument:

2

3

4

5

1

		Fig. 2.5.1 – Rear Panel
1.	Lamp access panel	Allows access to lamp when replacement is necessary
2.	Power switch	On/off switch for the unit
3.	Power in socket	Connection socket for power supply unit
4.	RS232 serial port	Connection to a PC or external serial printer
5.	Output sockets	Analogue output

2.6FRONT PANEL

The image below shows the front panel of the instrument:

1

2 5

4

3

Fig. 2.6.1 – Front Panel

1.Integral printer (optional accessory)

2.Keypad

3.USB memory stick slot

4.Instrument lid

5.Display

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SECTION 3 – Theory and Practice of Spectroscopy Measurements

3.1THEORY OF SPECTROSCOPY MEASUREMENT

UV-visible spectroscopy is the measurement of the absorbance of light at a specific wavelength in a sample. This is used to identify the presence and concentration of molecular entities within the sample. The Beer-Lambert law is used to relate the absorption of light to the properties of the sample through which the light is travelling through. The Beer-Lambert law states that:

A is the absorbance

is the molar absorption coefficient (l mol-1cm-1) c is the concentration (mol l-1)

l is the path length (cm)

This law shows that absorbance is linear to concentration but this is only true for low concentrations. For absorbance levels above 3 the concentration starts to move away from the linear relationship.

Transmittance is the proportion of the light which passes through the sample:

						Where:
Io					It	Lo	is the incident light
						lt	is the transmitted light
						l	is the path length

l

Therefore: T = It

Io

Absorbance is inversely related to transmittance:

A = log 1

T

3.2SPECTROSCOPY MEASUREMENT

There are four main components of a spectrophotometer. These are a light source to emit a high and constant amount of energy over the full wavelength range; a method for separating the light into discreet wavelengths; a sample holder and a light detector.

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The optical layout of the 7310 and 7315 spectrophotometers is shown below:

Lamp

Entrance slit

Grating

Collimator mirror

Exit Slit

Detector

Sample	Collecting Lens

Figure 3.2.1 – Diagram of light path

The light from the pre-focused tungsten halogen (7310) or pre-aligned xenon (7315) lamp is focused onto the grating, with 1200 lines per millimeter, which separates the light into discreet wavelengths. The diffracted spectrum of light then passes through a further slit and lens arrangement before passing through the sample in the sample chamber from left to right. The light which is not absorbed by the sample is transmitted through a collecting lens and onto the signal detector. The photo-diode detector used is mounted directly onto the detector PCB and the output is used to calculate the % transmittance. The result is displayed either as % transmittance or absorbance on the instrument display.

3.3GOOD PRACTICE GUIDELINES

1.For optimum performance all spectrophotometers should be sited in a clean, dry, dust free atmosphere. When in use ambient temperature and light levels should remain as constant as possible.

2.If required adherence to Standard Operating Procedures (SOP) and Good Laboratory Practice (GLP) should be monitored with regular calibration checks and a suitable Quality Control (QC) programme.

3.The sample chamber lid must be fully closed during measurement and before any readings are recorded or printed.

4.The correct selection of sample containers is imperative for accurate and reproducible results:

a)Check that the material of the sample container is compatible with the wavelengths to be used for measurement. In general glass can only be used down to 360nm or 320nm depending on quality. Standard plastic cuvettes can be used down to 320nm. Special UV versions can be used down to 260nm. Below this level quartz cuvettes must be used.

b)Plastic disposable cuvettes should only be used ONCE.

c)Glass cuvettes should be thoroughly cleaned after use. Discard when scratches become evident on optical surfaces.

d)Care should be taken when selecting semi-micro or micro cuvettes. The cuvette window on the

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inner chamber (the area filled with sample) must be wider than the aperture in the sample holder or light will reach the detector without passing through the sample. In this case, semi-micro or micro cuvettes with self-screening black surrounds must be used or, alternative holders for these cuvettes should be used.

e)Glass test tubes and other sample tubes should be used with care. Where possible, matched tubes should be used and any index mark set to the correct position before measurements are made.

f)Ensure any sample containers used are compatible with the constituents of both the samples and standards they are to hold. Plastic cuvettes are not compatible with organic solvents.

g)All sample containers must be handled with care; by the top, bottom and non-optical surfaces only. Any finger marks evident must be removed by a suitable cleaning process.

h)Flow-through cuvettes must be selected with care and consideration for the sample type, sample volume, pumping system, rinse, sample and waste handling to be used.

5.Samples and standards should not be stored in open cuvettes or sample containers as evaporation will change the value and lead to staining of the walls which may be irreversible. If stored in stoppered and sealed cuvettes, they should be filled with little or no air space and the values regularly checked against a reference standard or quality control material.

6.Samples should be allowed to equilibrate to ambient temperature before measurement (unless a suitable temperature controlled sample holder is in use). Temperature change during measurement may cause air bubbles to form on the walls of the sample holder. This is a common cause of drift during measurement.

7.In the preparation of samples and standards high grade borosilicate glass and AR grade chemicals and reagents must be used. Good quality deionised water or other suitable solvents must be used for dissolving or diluting samples, chemicals and reagents.

8.All measurements require calibration to a blank, for maximum accuracy this should be prepared with care using the same deionised water or solvent used for dissolving or diluting the sample. Where reagents are added to the sample to produce a colour proportional to its concentration a ‘sample based’ blank should be used. In this case the blank should consist of all reagents or chemicals to be used, except the sample which will produce the colour to be measured.

9.Deviations from the Beer-Lambert Law may occur at high and low concentrations giving non-linear response during sample concentration measurements. For all new methods a linear range should be defined by the preparation of a calibration curve. The quantitation mode may be used to construct such a curve against which sample results are automatically measured.

10.Cuvettes and sample holders must be filled to a minimum level which covers the light path. All Jenway spectrophotometers have a beam height of 15mm.

11.The instrument must be calibrated to zero absorbance/100% transmittance prior to taking readings. In the spectrum measurement mode a baseline scan must be performed before performing a sample scan.

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SECTION 4 – Instrument Setup

4.1NAVIGATING AND SCREEN SETUP

The main menu screen is displayed below.

		Kinetics
Spectrum		measurement
measurement		mode
mode
	7310	Concentration
		measurement
		mode
Photometrics	09:02
measurement		Instrument
mode
		settings menu

Back key		Time and date menu	Quantitation measurement mode

Fig 4.1.1 – Home Screen

To navigate around the spectrophotometer screen press the soft keys adjacent to icons displayed on the screen. In the main menu either of the two soft keys adjacent to the measurement mode icon can be pressed to access the mode. There is a back key which returns to the previous menu without saving any changes.

The main menu screen provides access to all five measurement modes, the time and date menu and the instrument settings menu. The measurement modes are spectrum, photometrics, quantitation, concentration and kinetics. The instrument settings menu enables access to settings lock, security codes, method lock, mode selection, user ID, screen contrast and lamp save menus.

0.000ABS

100.0%T

400nm

09:02

Minimal Operating Menu

0.000ABS

100.0%T

400nm

09:02

Expanded Operating Menu (Photometrics measurement mode)

All of the measurement modes open initially into a minimal operating menu. This menu allows calibration and simple readings to be taken without changing any measurement parameters. Pressing the key adjacent to the JW icon opens the expanded operating menu.

This menu enables changes to measurement parameters and settings to be made. Depending on the mode, the measurement parameters can be accessed through the settings menu which is displayed in the top right hand corner of the screen. The only mode where this function is not available is the photometrics mode; instead a toggle icon is displayed which is used to change the primary and secondary displays.

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The measurement settings can be accessed through the utility toolbar displayed on the left hand side of the expanded operating menu. This toolbar provides the same functions in all of the measurement modes. The utility toolbar enables access to printing, print setup options, opening, saving and deleting results and methods and autologging options. For more details on the different functions of the utility toolbar refer to section 10.

4.2TIME AND DATE

7310

09:02

1 4 : 5 3

3011 09

4.3INSTRUMENT SETTINGS MENU

The time and date menu enables the current time and date to be set. This information will be saved on all results and displayed on printouts. The time and date menu can be accessed from the main menu by holding the key below the time and date icon for 2 seconds. Pressing the key once cycles the display between time and date.

In the time and date menu to set the time press the key adjacent to the clock icon. Select the digit to be changed using the keys at the bottom of the screen. Use the keys adjacent to the arrow icons to increase or decrease the number. The clock function uses a 24 hour format.

In the time and date menu to set the date press the key adjacent to the calendar icon. Select the digit to be changed using the keys at the bottom of the screen. Use the keys adjacent to the arrow icons to increase or decrease the number. The date format can be displayed as either European dd/mm/yy or American mm/dd/yy. To change between the two formats press the key below the toggle icon. Once the current time and date have been set press the key adjacent to the tick icon to save the changes. To exit this menu without saving any changes press the back key and the screen will return to the main menu.

The instrument settings menu is accessed by pressing the key below the instrument settings icon in the main menu. This menu enables access to settings lock, security code, method lock, mode selection, diagnostics, user ID, screen contrast and lamp save menus. The tick icon saves any changes made and returns to the main menu.

Settings lock											Tick icon
Security code											User ID
Method lock											Screen
											contrast
Mode selection
											Lamp save
Diagnostics
							Fig 4.3.1 - Settings Menu

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4.4SECURITY AND SETTING PASSWORDS

4.4.1Setting Security Codes

The security code function enables a security code to be set to lock the instrument settings and measurement mode settings. The security code is not specific to the user ID but is designed to enable an administrator to control either the instrument or protocols. The security code menu is accessed through the instrument settings menu.

In the instrument settings menu press the key adjacent to the security code icon. Using the keys at the bottom of the screen select the digit to be changed. Use the keys adjacent to the arrow icons to increase or decrease the selected number. Once the preferred code has been set

6 6 0 press the key adjacent to the tick icon to save the security code.

4.4.2Settings lock

The settings lock function enables the instrument and measurement mode settings to be locked to prevent any changes to the measurement parameters or instrument settings. The only exceptions to this are that the user ID and contrast can be changed when the settings lock is active.

0 0 0

4.4.3Method Lock

The settings lock function is accessed through the instrument settings menu by pressing the key adjacent to the open padlock icon. One press will lock the settings instantly. To unlock the settings press the key again. This will open the security code menu as detailed in section 4.4.1. The previously set security code must be entered to unlock the settings. When the settings lock is active methods can still be opened, deleted and saved but the method parameters cannot be changed.

To enter the security code use the keys at the bottom of the screen to select the digit to be changed. Use the keys adjacent to the arrow icons to increase or decrease the selected number. Once the correct security code has been entered press the key adjacent to the tick icon. The settings are now unlocked.

If the settings are locked before the security code has been set a default code of 660 will unlock the settings.

When the method lock is active the method selection menu is disabled in all the measurement modes therefore methods cannot be opened, deleted or saved. However the measurement parameters of the currently loaded method can be changed. The method lock function is accessed through the instrument settings menu by pressing the key adjacent to the method lock icon.

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One press will lock the methods instantly. To unlock the methods press the key adjacent to the method lock icon again. The methods are now unlocked. If the settings lock is active this must be disabled before the method lock can be activated or deactivated.

In all the measurement modes if a user tries to save changes to a method when the method lock is active the padlock icon flashes on the screen and changes cannot be saved.

4.5MODE SELECTION

								The mode selection function enables access to the various
								measurement modes to be restricted. The required modes
								can be selected and the settings lock activated to prevent
								other users from accessing the deactivated modes. The
								mode selection function can be accessed through the
								instrument settings menu by pressing the key adjacent
								to the mode selection icon.
								The measurement mode icons which are displayed on the
								main menu are identified with a mode shown icon. The
								mode icons which are not displayed on the main menu
								are identified with a mode not shown icon.
								To change a mode from displayed to restricted or vice
								versa press the key adjacent to the measurement mode
								icon. Once the required modes have been selected press
								the key adjacent to the tick icon to save the changes.
								The selected measurement modes will be displayed on
			7315					the main menu.

09:02

4.6DIAGNOSTICS

Go to WL	0.00
	500.0 nm
ON	READ

The diagnostic function allows simple checks to be carried out on the instrument. The wavelength can be changed, the lamp can be turned on and off and a sensitivity reading can be performed.

To exit this function without performing any checks press the back key.

4.7GLP SETTINGS

In addition to the time and date settings this instrument also has a user ID function. This function enables an individual three digit ID number to be set. This will be displayed on all printouts and saved results.

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0 0 1

4.8SCREEN CONTRAST

The user ID function can be accessed through the instrument settings menu by pressing the key adjacent to the user ID icon. Use the keys at the bottom of the screen to select the digit to be changed. Use the keys adjacent to the arrow icons to increase or decrease the number. Once the preferred user ID has been set press the key adjacent to the tick icon to save and return to the instrument settings menu.

The screen contrast function enables the brightness of the screen to be set. In the instrument settings menu press the key adjacent to the screen contrast icon. Use the keys below the arrow icons to increase or decrease the screen contrast. Once the required contrast level has been reached press the key adjacent to the tick icon to save and return to the instrument settings menu.

4.9LAMP SAVE

This function is only available on the 7310 visible spectrophotometer which uses a tungsten halogen lamp.

The lamp save function enables the time in minutes to be set after which the lamp will be turned off following a period of no lamp activity, i.e. no readings have been performed. This function is accessed through the instrument settings menu by pressing the key adjacent to the lamp save icon.

When this menu is first accessed the lamp save is turned off. To activate the lamp save function press the key below the lamp save icon. To deactivate the lamp save function press the key below the lamp save icon.

							The default minimum time is set to 30 minutes. Select
							the digit to be changed using the keys at the bottom of
							the screen. Use the keys adjacent to the arrow icons to
							increase or decrease the number. Once the required time
							in minutes has been set press the key adjacent to the tick
			3				icon to save and return to the instrument settings menu.
	0			0

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0.000ABS

100.0%T

400nm

09:02

0.000ABS

100.0%T

400nm

09:02

16:30

09:02

0.000ABS

100.0%T

400nm

09:02

The time set will begin to count down when there is no lamp activity. When the count down is complete the lamp and the fan will be turned off and the lamp save icon is shown in all the measurement modes. To bring the instrument out of the lamp save in order to perform a measurement press the key below the lamp save icon. The lamp and fan will be turned back on and the lamp will begin to warm up.

The lamp cold icon is displayed adjacent to the calibrate to zero icon in the measurement mode. The time needed for the lamp to warm up is five minutes.

To view the remaining warm up time press the key below the lamp cold icon. Calibration and measurements can be performed whilst the lamp is warming up but these results may not be accurate.

Once the warm up time of five minutes is complete the lamp cold icon disappears.

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SECTION 5 – Photometrics

The photometrics measurement mode enables simple measurements of absorbance and % transmittance to be performed. The sample is measured at one wavelength and at one point in time. There are no post measurement calculations available in this measurement mode.

5.1MODE SPECIFIC PARAMETERS

0.000ABS

100.0%T

400nm

The photometrics minimal operating menu enables calibration to zero absorbance/100% transmittance and simple readings to be taken without changing any measurement parameters. Pressing the key adjacent to the JW icon opens the expanded operating menu.

09:02

Minimal Operating Menu

100.0%T

0.000ABS

400nm

09:02

Expanded Operating Menu

The photometrics expanded operating menu enables measurement parameters to be changed. The utility toolbar on the left hand side of the screen enables access to printing, print setup options, results, methods and autologging options. For more details on the different functions of the utility toolbar refer to section 10.

Print/print settings

Toggle

100.0

%T

Results selection menu

Increase wavelength

Method selection menu

ABS

0.000

Autolog menu

Decrease wavelength

400nm

09:02

Calibrate to zero

Measure sample

Fig 5.1.1 - Expanded Operating Menu

5.2METHOD SET UP

0.000ABS

100.0%T

400nm

This measurement mode is very simple and the only parameters which can be adjusted are the wavelength and the display format.

09:02

25

The toggle icon enables the large primary display to be set to show the absorbance or % transmittance. To change the primary and secondary displays press the key adjacent to the toggle icon. Repeat presses will cycle the displays between absorbance or % transmittance.

5.2.1Selecting a Wavelength

The wavelength can be adjusted in the expanded operating menu by using the keys adjacent to the arrow icons to increase or decrease the wavelength. Once the required wavelength has been selected a calibration can be performed.

5.3CALIBRATION

100.0%T

0.000ABS

400nm

09:02

The calibration must be performed at the same wavelength at which the sample will be measured. Insert a cuvette containing the blank solution into the sample chamber and close the instrument lid. Press the key below the calibrate to zero absorbance icon. This sets the instrument to zero absorbance and 100% transmittance.

Once the calibration is complete the measure sample icon appears and the sample can be measured. If the wavelength is adjusted before a sample is measured the measure sample icon will disappear and the instrument must be calibrated again at the new wavelength.

5.4SAMPLE MEASURMENT

81.7%T

0.088ABS

400nm

09:02

It is not possible to measure a sample before the instrument has been calibrated at the selected wavelength. Once the calibration has been performed the measure sample icon is displayed and a sample can be measured. Remove the cuvette containing the blank solution and place a cuvette containing the sample to be measured in the sample holder. Close the instrument lid and press the key below the measure sample icon.

Once the measurement is complete the photometric result will be shown on the screen. Subsequent samples can be measured in the same way. If the wavelength is adjusted between sample measurements then the instrument must be calibrated again before more samples can be measured.

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SECTION 6 – Concentration

The concentration measurement mode enables simple measurements of absorbance and concentration to be performed. In this measurement mode it is possible to calibrate against a standard of a known concentration or use a known factor. The sample is measured at one wavelength at one point in time. There are no post measurement calculations available in this measurement mode.

6.1MODE SPECIFIC PARAMETERS

0.000ppm

0.000ABS

400nm

The concentration minimal operating menu enables calibration to zero absorbance and simple readings to be taken without changing any measurement parameters. Pressing the key adjacent to the JW icon opens the expanded operating menu.

09:02

Minimal Operating Menu

0.000ppm

0.000ABS

400nm

09:02

Expanded Operating Menu

The concentration expanded operating menu enables measurement parameters to be changed. The utility toolbar on the left hand side of the screen enables access to printing, print setup options, results, methods and autologging options. For more details on the different functions of the utility toolbar refer to section 10. The settings icon enables the wavelength, units, resolution, standard or factor to be set.

Print/print settings

Settings

0.000

ppm

Results selection menu

Increase wavelength

0.000

ABS

Method selection menu

400nm

Decrease wavelength

Autolog menu

09:02

Calibrate to zero

Measure sample

Fig 6.1.1 - Expanded Operating Menu

6.2METHOD SETUP

6.2.1Selecting a Wavelength

0.000ppm

0.000ABS

400nm

The wavelength can be adjusted in the expanded operating menu or in the settings menu. To adjust the wavelength in the expanded operating menu use the keys adjacent to the arrow icons to increase or decrease the wavelength.

09:02

27