Page 1
o
r usell Culture Consumables
E
N)ns for use
Eppendorf Cell Culture
Consumables
Instructions for use
Page 2
Copyright© 2014 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.
Eppendorf
Registered trademarks are not marked in all cases with ™ or
®
and the Eppendorf logo are registered trademarks of Eppendorf AG.
®
in this manual.
0012 584.229-03/072014
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Eppendorf Cell Culture Consumables
Table of co nt en ts
English (EN)
Table o f contents
1Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.1 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2.2 Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2.1 Filter cap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2.2 Plug-seal cap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.3 Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3Safety precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
4Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
5Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.1 Cell Culture Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.1.1 Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.1.2 Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.1.3 Flasks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3
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Operating instructions
Eppendorf Cell Culture Consumables
4
English (EN)
1Operating instructions
1.1 Using this manual
Before using the consumables for the first time, read these instructions for use and the
operating manual of the device that you use the consumables with. You can find the
current version of the operating manual on the Internet at www.eppendorf.com/
worldwide. These instructions for use do not replace the device operating manual.
2Product description
The Eppendorf Cell Culture Consumables are suitable for the growth and cultivation of
eukaryotic cells and also for the storage and microscopy of cells.
They are available as Multiwell plate, flasks and dishes with tissue culture-treated (TCT)
for adherent cells and untreated surfaces for suspension cells.
Eppendorf Cell Culture Consumables can be used in training, routine and research
laboratories in the fields of life sciences, industry, or chemistry. The product is intended
for research purposes only. Any other use is not warranted by Eppendorf. Not intended
for use in diagnostic or therapeutic procedures.
Eppendorf Cell Culture Consumables may only be used by skilled personnel who have
been trained in the areas mentioned above.
2.1 Plates
•Sealing:
The plates are delivered with a lid. The plates can also be sealed with Eppendorf
Storage Film or Eppendorf Storage Foil.
•Photometric measurements:
The plates are suitable for photometric measurements and cell-based assays.
•Centrifugation:
The plates are suitable for centrifugation. The centrifugation of plate stacks is also
possible (see Technical data on p . 7).
•Growth area:
The optimized growth area of the 96-well plate results in an increased yield of the
number of cells.
Mind the comparatively large growth area/well. Note that, depending on the cell
system, more cells may be required for seeding in order to achieve the same cell
density (cell number limit/mm
2
) as in other 96-well plates.
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Eppendorf Cell Culture Consumables
1
Product description
English (EN)
•Prevention of the edge effect:
The 96-well plate has a peripheral moat
which can be filled prior to cell seeding
in order to prevent the edge effect.
Before filling the cells into the wells,
pipette 5 mL water/buffer into the
peripheral moat.
In order to make the filling of the moat
easier, you can reduce the surface
tension of the liquid by adding serum,
proteins or detergent. Additional
information is available in Eppendorf
Application Note 326.
In addition, it is also possible to fill the inter-well space within the plate with 8 to 10 mL
of liquid. This delays the cooling of the medium in the wells when the plate is used
outside of the incubator.
•Additional wells:
For the growth of very small numbers of
cells, the 6-well plate has 2 small,
additional wells (1) at the center.
These wells can be used for a direct
sample preparation on the plate (e.g.
preparing of coloring or antibodies
solutions).
5
2.2 Flasks
The flasks are suitable for centrifugation (see Tech ni cal data on p . 7) .
2.2.1 Filter cap
When fully closed, flasks with a filter cap are suitable for the growth of cells as the gas
exchange takes place continuously via the filter.
2.2.2 Plug-seal cap
The flasks can be completely closed with the plug-seal cap.
To incubate the cells, turn the cap to the vent position (Vent). Securely engaging the cap
in the Vent position ensures that the cells are properly vented.
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Safety precautions
Eppendorf Cell Culture Consumables
6
English (EN)
2.3 Dishes
• Closing of dishes:
The dishes are delivered with a lid.
•Handling ring:
The dishes have a circumferential handling ring which facilitates the opening and
closing as well as the safe transport of the dishes. This reduces the risk of liquids
overflowing and therefore the risk of contamination.
3Safety precautions
WAR NIN G! Ris k o f cont ami nation .
Consumables are only sterile in sealed packaging.
Please check that the packaging is undamaged.
Observe the expiry date printed on the packaging.
Only open the packaging immediately before use.
Check the welds of flasks.
Only use visually perfect and undamaged items.
Do not use liquid nitrogen. The consumables could get damaged or the caps
could burst open during thawing.
WAR NING! Dam age s t o heal th due to in fec tious liq uid s a nd pat hog enic
germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
Consult the "Laboratory Biosafety Manual" (Source: World Health
Organization, Laboratory Biosafety Manual, as amended) for comprehensive
regulations on the handling of risk group II germs or biological materials).
The Eppendorf Cell Culture flasks are not suitable for shipping living cells.
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Eppendorf Cell Culture Consumables
Technical d at a
English (EN)
4Technical data
Tab. 4-1: Plates
Unit 6-well 12-well 24-well 48-well 96-well
Working volume mL 3.0 to 5.0 1.0 to 2.0 0.5 to 1.0 0.3 to 0.5 0.1 to 0.2
Theoretical total
mL 16.0 6.8 3.6 1.5 0.4
volume
Growth area*/well
mm
2
940,3
391.1 208.9 85.6 37.0
Extra well:
0.05 to 0.08
Well diameter mm 34.6
22.4 16.2 10.4 6.8
Extra well:
27.5
Length × width ×
height (l × w × h)
mm 127.8 × 85.5 × 20.0 127.8 × 85.5
× 14.4
Height with lid mm 23.2 17.6
*For the initial seeding of cells, take into account the growth area in order to support
optimal cell growth.
Tab. 4-2: Dishes
Unit 35 mm 60 mm 100 mm
Working volume mL 2.0 to 3.0 3.0 to 5.0 8.0 to 10.0
Theoretical total
mL 8.1 26.6 99.2
volume
Growth area
Diameter × height
2
9.5 21.9 56.8
cm
mm 36.7 × 10.8 59.7 × 14.0 93.8 × 19.4
(d × h)
Height with lid mm 12.5 15.7 21.3
7
Tab. 4-3: Flasks
Unit T-25 T-75 T-175
Working volume mL 3.0 to 7.0 8.0 to 20.0 20.0 to 30.0
Theoretical total
mL 83.6 279.8 662.1
volume
Growth area [cm²]
Length × width ×
2
26.9 77.9 179.5
cm
mm 99.0 × 56.0 × 37.1 163.0 × 82.0 × 46.6 230.0 × 122.0 × 48.0
height (l × w × h)
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Technical d at a
Eppendorf Cell Culture Consumables
8
English (EN)
Tab. 4-4: Materials
Material: Polystyrene, meets the requirements of USP Class VI
Surface: Tissue culture treated or non-treated
Tab. 4-5: Dimensions
96-well plate Meets the Standards ANSI/SLAS* 1-2004 through
ANSI/SLAS* 4-2004
6-/12-/24-/48-well plate: Meets the Standard ANSI/SLAS* 1-2004:
Microplates–Footprint Dimensions
*SLAS: Society of Laboratory Automation and Screening
Tab. 4-6: Centrifugability
Plates*: centrifugatable up to 2 500 x g. Centrifugatable in stack
(4 plates or 5 96 well plates) up to 300 x g.
Dish: Cannot be centrifuged
Flask: Can be centrifuged with the suitable adapters;
refer to the manufacturer's instructions.
The centrifugation stability of each plate type is generally depending on the centrifuge
and its accessories, the ambient conditions and the liquid used.
Tab. 4-7: Temperature conditions, resistance
Operating temperature: -86°C to 60°C
Storage before use: Store dry at room temperature. Protect from sunlight and
UV rays
Tab. 4-8: Certificates
Certificates: Leachables, heavy metals, production conditions, cytotoxicity.
The certificates can be found at www.eppendorf.com
.
Lot-specific certificates: Free from: RNase/DNase, human DNA, bacterial DNA.
Sterility: Cell growth on TCT surfaces, physical surfaces.
Lot-specific certificates can be downloaded at
www.eppendorf.com
.
Further technical data can be found online in the "Technical Data Sheets" at
www.eppendorf.com/worldwide
.
Page 9
5Ordering information
5.1 Cell Culture Consumables
5.1.1 Plates
Eppendorf Cell Culture Consumables
Ordering information
English (EN)
9
Order no.
(International)
0030 720.113 0030720113 60 pieces, individually wrapped
0030 720.121 0030720121 200 pieces, 20 bags of 10 pieces
0030 720.016 0030720016 60 pieces, individually wrapped
0030 721.110 0030721110 60 pieces, individually wrapped
0030 721.012 0030721012 60 pieces, individually wrapped
0030 722.116 0030722116 60 pieces, individually wrapped
0030 722.019 0030722019 60 pieces, individually wrapped
0030 723.112 0030723112 60 pieces, individually wrapped
0030 723.015 0030723015 60 pieces, individually wrapped
0030 730.119 0030730119 80 pieces, individually wrapped
0030 730.127 0030730127 200 pieces, 20 bags of 10 pieces
0030 730.011 0030730011 80 pieces, individually wrapped
Order no. (North
America)
Description
Eppendorf Cell Culture Plate, 6-Well
TC treated
Eppendorf Cell Culture Plate, 6-Well
non-treated
Eppendorf Cell Culture Plate, 12-Well
TC treated
Eppendorf Cell Culture Plate, 12-Well
non-treated
Eppendorf Cell Culture Plate, 24-Well
TC treated
Eppendorf Cell Culture Plate, 24-Well
non-treated
Eppendorf Cell Culture Plate, 48-Well
TC treated
Eppendorf Cell Culture Plate, 48-Well
non-treated
Eppendorf Cell Culture Plate, 96-Well
TC treated
Eppendorf Cell Culture Plate, 96-Well
non-treated
Page 10
Ordering information
Eppendorf Cell Culture Consumables
10
English (EN)
5.1.2 Dishes
Order no.
(International)
0030 700.112 0030700112 300 pieces, 30 bags of 10 pieces
0030 700.015 0030700015 300 pieces, 30 bags of 10 pieces
0030 701.119 0030701119 300 pieces, 30 bags of 10 pieces
0030 701.011 0030701011 300 pieces, 30 bags of 10 pieces
0030 702.115 0030702115 300 pieces, 30 bags of 10 pieces
0030 702.018 0030702018 300 pieces, 30 bags of 10 pieces
Order no. (North
America)
Description
Eppendorf Cell Culture Dish, 35 mm
TC treated
Eppendorf Cell Culture Dish, 35 mm
non-treated
Eppendorf Cell Culture Dish, 60 mm
TC treated
Eppendorf Cell Culture Dish, 60 mm
non-treated
Eppendorf Cell Culture Dish, 100 mm
TC treated
Eppendorf Cell Culture Dish, 100 mm
non-treated
5.1.3 Flasks
Order no.
(International)
0030 710.126 0030710126 Filter cap, 192 pieces, 24 bags of 8
0030 710.118 0030710118 Plug-seal cap, 192 pieces, 24 bags of 8
0030 710.029 0030710029 Filter cap, 192 pieces, 24 bags of 8
0030 710.010 0030710010 Plug-seal cap, 192 pieces, 24 bags of 8
Order no. (North
America)
Description
Eppendorf Cell Culture Flask, T-25
TC treated
pieces
pieces
Eppendorf Cell Culture Flask, T-25
non-treated
pieces
pieces
Page 11
Eppendorf Cell Culture Consumables
Ordering information
English (EN)
11
Order no.
(International)
Order no. (North
America)
Description
Eppendorf Cell Culture Flask, T-75
TC treated
0030 711.122 0030711122 Filter cap, 80 pieces, 16 bags of 5
pieces
0030 711.114 0030711114 Plug-seal cap, 80 pieces, 16 bags of 5
pieces
Eppendorf Cell Culture Flask, T-75
non-treated
0030 711.025 0030711025 Filter cap, 80 pieces, 16 bags of 5
pieces
0030 711.017 0030711017 Plug-seal cap, 80 pieces, 16 bags of 5
pieces
Eppendorf Cell Culture Flask, T-175
TC treated
0030 712.129 0030712129 Filter cap, 48 pieces, 12 bags of 4
pieces
0030 712.110 0030712110 Plug-seal cap, 48 pieces, 12 bags of 4
pieces
Eppendorf Cell Culture Flask, T-175
non-treated
0030 712.021 0030712021 Filter cap, 48 pieces, 12 bags of 4
pieces
0030 712.014 0030712015 Plug-seal cap, 48 pieces, 12 bags of 4
pieces
Page 12
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