Eppendorf Cell Culture Consumables User Manual

o

r usell Culture Consumables

E

N)ns for use

Eppendorf Cell Culture
Consumables

Instructions for use

Copyright© 2014 Eppendorf AG, Hamburg. No part of this publication may be reproduced
without the prior permission of the copyright owner.

Eppendorf

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®

and the Eppendorf logo are registered trademarks of Eppendorf AG.

®

in this manual.

0012 584.229-03/072014

Eppendorf Cell Culture Consumables

Table of co nt en ts

English (EN)

Table o f contents

1Operating instructions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

2Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

2.1 Plates. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

2.2 Flasks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2.2.1 Filter cap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2.2.2 Plug-seal cap . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2.3 Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

3Safety precautions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

4Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

5Ordering information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

5.1 Cell Culture Consumables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

5.1.1 Plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

5.1.2 Dishes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

5.1.3 Flasks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

3

Operating instructions

Eppendorf Cell Culture Consumables

4

English (EN)

1Operating instructions

1.1 Using this manual

Before using the consumables for the first time, read these instructions for use and the
operating manual of the device that you use the consumables with. You can find the
current version of the operating manual on the Internet at www.eppendorf.com/
worldwide. These instructions for use do not replace the device operating manual.

2Product description

The Eppendorf Cell Culture Consumables are suitable for the growth and cultivation of
eukaryotic cells and also for the storage and microscopy of cells.

They are available as Multiwell plate, flasks and dishes with tissue culture-treated (TCT)
for adherent cells and untreated surfaces for suspension cells.

Eppendorf Cell Culture Consumables can be used in training, routine and research
laboratories in the fields of life sciences, industry, or chemistry. The product is intended
for research purposes only. Any other use is not warranted by Eppendorf. Not intended
for use in diagnostic or therapeutic procedures.

Eppendorf Cell Culture Consumables may only be used by skilled personnel who have
been trained in the areas mentioned above.

2.1 Plates

•Sealing:
The plates are delivered with a lid. The plates can also be sealed with Eppendorf

Storage Film or Eppendorf Storage Foil.

•Photometric measurements:
The plates are suitable for photometric measurements and cell-based assays.

•Centrifugation:
The plates are suitable for centrifugation. The centrifugation of plate stacks is also

possible (see Technical data on p . 7).

•Growth area:
The optimized growth area of the 96-well plate results in an increased yield of the

number of cells.

Mind the comparatively large growth area/well. Note that, depending on the cell
system, more cells may be required for seeding in order to achieve the same cell
density (cell number limit/mm

2

) as in other 96-well plates.