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nual
830
N)
manual
Biomaster® 4830
Operating manual
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Copyright © 2013 Eppendorf AG, Hamburg. No part of this publication may be
reproduced without the prior permission of the copyright owner.
Trademarks
Eppendorf® and the Eppendorf logo and are registered trademarks of Eppendorf AG,
Hamburg, Germany.
®
BIOMASTER
Germany.
Registered trademarks are not marked in all cases with ™ or
and Mastertip® are registered trademarks of Eppendorf AG, Hamburg,
®
in this manual.
4830 900.050-02/102013
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Table of contents
Biomaster® 4830
English (EN)
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.2.1 Danger symbol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.2.2 Danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2 Product description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1 Operating principle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
3 Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3.2 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
3.3 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
4 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.1 Setting the volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.2 Tip take-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.3 Aspirating liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.4 Dispensing liquid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
4.5 Ejecting the Mastertip . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
5 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.1 Testing the volume . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.2 Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
5.3 Cleaning and decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
5.4 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3
6 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
6.1 Test conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
7 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
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Operating instructions
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Biomaster® 4830
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Also
observe the instructions for use of the accessories.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
You will find the current version of the operating manual for all available languages on
our webpage under www.eppendorf.com
.
1.2 Danger symbols and danger levels
The safety instructions of this operating manual indicate the following danger symbols
and danger levels:
1.2.1 Danger symbol
Biohazard Toxic substances
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to severe injuries or death.
WAR NI NG May lead to severe injuries or death.
CAUTION May lead to light to moderate injuries.
Notice May lead to material damage.
1.3 Symbols used
Depiction Meaning
1.
2.
Actions without a specified order
• List
Text Display text or software text
Actions in the specified order
Additional information
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Product description
Biomaster® 4830
English (EN)
2 Product description
2.1 Operating principle
The Biomaster is a pipette designed for very sensitive testing in the volume range of 1 μL
– 20 μL. It can be used with variable settings and without entrainment of residues.
Its tip, the Mastertip, can be mounted with one hand and disposes of an integrated piston.
By inhibiting the formation of aerosols, the Mastertip excludes sample contamination.
The Biomaster is UV-resistant and autoclavable. Mastertips are autoclavable.
Please refer to the section on sterilization before autoclaving the Biomaster or the
Mastertip (121 °C, 20 min).
2.2 Main illustration
5
1
2
1 Control button
2 Volume locking button
3 Mastertip
3
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Safety
6
Biomaster® 4830
English (EN)
3 Safety
3.1 Intended use
The Biomaster is a lab device which, in combination with a Mastertip, is intended for
dispensing aqueous solutions in the volume range of 1 μL – 20 μL. In vivo applications
(applications in or on the human body) are not permitted.
The Biomaster may only be operated by trained specialists. All users must have read the
operating manual carefully and familiarized themselves with the device's mode of
operation.
3.2 Warnings for intended use
WARNING! Damages to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national
regulations, the biological security level of your laboratory, the material safety
data sheets, and the manufacturer's application notes.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of
risk group II or higher, please refer to the "Laboratory Biosafety Manual"
(Source: World Health Organization, current edition of the Laboratory Biosafety
Manual).
WARNING! Damage to health due to toxic, radioactive or aggressive chemicals.
Wear personal protective equipment.
Observe the national regulations for handling these substances.
Observe the material safety data sheets and manufacturer's application notes.
CAUTION! Danger to individuals due to careless use
Never point the opening of the device towards yourself or other people.
Only initiate liquid dispensing if it is safe to do so.
With any dispensing task please ensure that you do not endanger yourself and
other persons.
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Safety
Biomaster® 4830
English (EN)
3.3 Information on product liability
In the following cases, the designated protection of the device may be compromised.
Liability for any resulting property damage or personal injury is then transferred to the
operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by
Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
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Operation
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Biomaster® 4830
English (EN)
4 Operation
4.1 Setting the volume
1. Press and hold the volume catch.
2. Turn the control button to set the volume.
3. Release the volume catch.
The set volume is now secured against unintentional readjustment.
4.2 Tip take-up
Prerequisites
The Mastertip is placed in a tip box.
1. Attach the Biomaster to one Mastertip.
You will feel the Biomaster locking in place in the Mastertip.
Keep the Mastertip in the tip box after attaching it to the Biomaster.
2. Firmly press the control button down to the second stop.
The piston is now fixed to the Biomaster.
3. Release the control button.
The piston will slide back up inside the tip until it reaches its final position.
The system is now ready for operation.
4.3 Aspirating liquid
1. Press down the control button to the first stop.
2. Immerse the Mastertip vertically approx. 3 mm into the liquid.
3. Let the control button slide back slowly.
4. Hold the Mastertip against the inner wall of the vessel while pulling it out of the liquid.
Wipe off any drops on the outside with a lint-free cellulose tissue. Make sure not to
aspirate any liquid from the Mastertip when wiping off the drops.
4.4 Dispensing liquid
1. Hold the Mastertip against the inner wall of the vessel at an angle.
2. Press down the control button to the first stop.
3. Hold down the control button and hold the Mastertip against the vessel wall while
pulling it up.
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4.5 Ejecting the Mastertip
Press down the control button completely to eject the Mastertip.
Small amounts of residual liquid at the tip of the Mastertip are not part of the
dispensing volume and do not have any effect on precision and accuracy.
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Maintenance
Biomaster® 4830
English (EN)
5 Maintenance
5.1 Testing the volume
You can test the volume of the Biomaster by weighing the pipetted, demineralized water
with a sufficiently sensitive analytical balance:
The following components must have the same temperature:
• demineralized water
• weighing vessel
• pipette
• pipette tips
Weighings should be conducted at 20 °C – 27 °C.
During the measurement the temperature may not fluctuate by more than ±0.5 °C.
Divide the weight of the water by its density (0.9982 mg/μL at 20 °C) to calculate the
volume. This procedure is based on ISO 8655.
Regularly test the precision and accuracy of the Biomaster to avoid dispensing errors. You
will find an SOP (Standard Operation Procedure) for testing pipettes on our website at
www.eppendorf.com
.
5.2 Sterilization
NOTICE! Damage to device from incorrect handling.
Do not use any additional disinfectants, decontamination agents or sodium
hypochlorite during autoclaving or UV exposure.
When autoclaving, please ensure that the temperature does not exceed 121 °C.
Check the suitability of an agent and the manufacturer information about
resistance to chemicals before using a disinfectant or decontamination agents.
Also observe the material of the pipette.
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You can fully autoclave the Biomaster (at 121 °C, for 20 min). Before doing so, unscrew
and loosen the upper part of the device from its lower part by 2 turns. Make sure that the
Biomaster has completely dried and cooled down before reassembling the device after
autoclaving. The special plastic material of the housing allows a UV light treatment (up to
254 nm).
The Mastertips can also be autoclaved at 121 °C, 20 min. The temperature and time for
autoclaving must be strictly observed. Otherwise, the proper functioning of the device
cannot be guaranteed.
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Maintenance
10
Biomaster® 4830
English (EN)
5.3 Cleaning and decontamination
NOTICE! Damage to device from unsuitable cleaning fluids or sharp or pointed
objects.
Unsuitable cleaning fluids can damage the device.
Never use corrosive cleaning fluids, strong solvents or abrasive polishes.
Check the compatibility with the materials used.
Do not clean the device with acetone or organic solvents with a similar effect.
Do not clean the device with sharp objects.
Proceed as follows:
To remove any contamination on the outside, dampen a soft cloth with a mild detergent
and wipe the housing.
To disinfect the device, wipe the housing with isopropanol (70%).
No further maintenance work is required.
5.4 Decontamination before shipment
If you wish to return the device to Eppendorf AG or an Eppendorf AG service partner to be
checked or repaired, please note the following:
CAUTION! Use of a contaminated device may result in personal injuries and
damage to the device.
Clean and decontaminate the device in accordance with the cleaning
instructions before shipping or storage.
Hazardous substances are:
• solutions presenting a hazard to health
• potentially infectious agents
• organic solvents and reagents
• radioactive substances
• proteins presenting a hazard to health
•DNA
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1. Please note the information in the document "Decontamination certificate for product
returns".
You can find it as a PDF file on our webpage www.eppendorf.com
2. Enter the serial number of the Pipette in the decontamination certificate.
3. With the shipment please include the completed decontamination certificate for
product returns with the Pipette.
4. Send the pipette to Eppendorf AG or an Eppendorf AG service partner. The address
details of Eppendorf AG can be found on the last page of this operating manual. The list
of service partners can be found at www.eppendorf.com/worldwide
Technical data
Biomaster® 4830
.
.
English (EN)
6 Technical data
6.1 Test conditions
Volume Systematic error Random error
2 μL ±6.0% ≤4.0%
3 μL ±5.0% ≤3.0%
5 μL ±4.0% ≤2.0%
10 μL ±3.0% ≤1.5%
20 μL ±2.0% ≤0.8%
Test conditions and test evaluation in accordance with ISO 8655, part 6. Test with
analytical balance with evaporation protection, approved by the board of weights and
measures.
Measurement errors do not apply to autoclaved Mastertips.
When using less than 2 μL for the test, the sample liquid and the experience of the user
have a critical influence on accuracy and precision. Therefore no limits are set for this
range.
• Number of determinations: 10
• Use of water in accordance with ISO 3696
• Test with a Mastertip
• Test at 20 °C – 27 °C ±0.5 °C
• Dispensing on the inner wall of the vessel
Technical specifications are subject to change.
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Ordering Information
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Biomaster® 4830
English (EN)
7 Ordering Information
Order no.
(International)
4830 000.017 022440501
0030 001.320 022354159 5 boxes of 96 = 480 pieces
3115 000.003 022444905
3115 600.019 022260588 For carousel (replacement)
3115 000.020 022444913
Order no. (North
America)
Description
Biomaster Kit
consisting of: 1 Biomaster pipette and 1 box of
96 Mastertips
Mastertips
tips plus piston, ready-for-use
Pipette carousel 3115
with 6 pipette holder for Research and Reference
Pipettes
Pipette holder
Pipette mount
For wall mounting (with adhesive backing) for
Research and Reference pipettes
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Your local distributor: www.eppendorf.com/contact
Eppendorf AG · 22331 Hamburg · Germany
eppendorf@eppendorf.com · www.eppendorf.com
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