Eppendorf 5430 R User Manual [en, es]
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Centrifuge 5430/5430 R
Operating manual
Manual de instrucciones
©
Copyright
2013 Eppendorf AG, Hamburg. No part of this publication may be reproduced without the prior
permission of the copyright owner.
Trademarks
Eppendorf
®
, the Eppendorf logo. CombiSlide®, and QuickLock® are registered trademarks of
Eppendorf AG, Hamburg, Germany.
Centriplus
Microtainer
Trademarks are not marked in all cases with ™ or
®
is a registered trademark of Millipore Corporation, Billerica, USA.
®
is a registered trademark of Becton Dickinson, Franklin Lakes, NJ, USA.
®
in this manual.
Operating manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Manual de instrucciones . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Declarations and Certificates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111
Menu structure of Centrifuge 5430/5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
Rotor F-35-6-30: Adapters for round-bottom tubes and blood taking systems . . . . . . . . . . . . . . . . . . . . . 128
5427 901.124-04/072013
Table of contents
Centrifuge 5430/5430 R
English (EN)
Operating manu alCentrifuge 5430/5430 Rseeon p.Fig.Tab.p.
English (EN)Operating manual
Table of contents
1 Operating instructions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.1 Using this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2 Danger symbols and danger levels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.1 Danger symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.2.2 Danger levels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.3 Symbols used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
1.4 Abbreviations used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2 Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.1 Main illustration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
2.2 Delivery package. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2.1 Centrifuge 5430. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.2.2 Centrifuge 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
2.3 Features. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2.4 Rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4.1 rcf display and calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
3
3 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.1 Intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.2 User profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.3 Application limits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.3.1 Declaration concerning the ATEX directive (94/9/EC) . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.3.2 Maximum service life for accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
3.4 Information on product liability . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.5 Warnings for intended use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.5.1 Personal injury or damage to the equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
3.5.2 Incorrect handling of the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
3.5.3 Incorrect handling of the rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
3.5.4 Extreme strain on the centrifuging tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3.5.5 Aerosol-tight centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
3.6 Safety notes on the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
4 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.1 Selecting the location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.2 Preparing installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
4.3 Installing the instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
5 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.1 Overview of operating controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5.2 Menu navigation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
5.3 Configure centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.3.1 Set menu language . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.3.2 Setting the date and time . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.4 Preparing for centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.1 Switching on the centrifuge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.2 Inserting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.3 Automatic rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.4 Manual rotor detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4.5 Loading the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.4.6 Closing the centrifuge lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Table of contents
Centrifuge 5430/5430 R
4
English (EN)
5.5 Cooling (only 5430 R) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.6 Centrifuging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.7 Standby mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.8 User instructions on rotors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.5.1 Temperature adjustment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.5.2 Temperature display . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.5.3 Temperature monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.5.4 FastTemp. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.5.5 FastTemp pro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
5.5.6 Continuous cooling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
5.6.1 Centrifugation with time setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
5.6.2 Centrifuging in continuous operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.6.3 Short-spin centrifugation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
5.6.4 Removing the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
5.8.1 Rotor F-35-6-30: rotor removal tool . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
5.8.2 Rotor A-2-MTP . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.8.3 Rotor FA-45-24-11-HS: using the special rotor key . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
5.8.4 QuickLock . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6 Operating controls and function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
6.1 Device menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
6.2 Settings in the device menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.2.1 Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.2.2 Use program keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
6.2.3 Other menu items . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
6.2.4 Settings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.1 Prepare cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2 Cleaning/disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
7.2.1 Cleaning and disinfecting the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.2.2 Cleaning and disinfecting the rotor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
7.3 Additional service instructions for Centrifuge 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7.4 Glass breakage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
7.6 Decontamination before shipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
8 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
8.1 General errors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
8.2 Error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
8.3 Emergency release . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
9 Transport, storage and disposal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
9.1 Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
9.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
9.3 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
10 Technical data. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.1 Power supply. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.2 Ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
10.3 Weight/dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
10.4 Application parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Table of contents
Centrifuge 5430/5430 R
English (EN)
11 Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
11.1 Centrifuge 5430 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
11.2 Centrifuge 5430 R . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
11.3 Rotors, rotor lids and seals . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
11.3.1 Rotors with QuickLock rotor lid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
11.3.2 Rotors with rotor lid thread. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
11.3.3 Rotors with rotor lid for attaching. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
11.4 Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
11.4.1 Adapter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
11.4.2 Other accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
11.4.3 Fuses for Centrifuge 5430. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
5
Table of contents
Centrifuge 5430/5430 R
6
English (EN)
Operating instructions
Centrifuge 5430/5430 R
English (EN)
1 Operating instructions
1.1 Using this manual
Read this operating manual completely before using the device for the first time. Please also note the
operating instructions for the accessories, if applicable.
This operating manual is part of the product. Thus, it must always be easily accessible.
Enclose this operating manual when transferring the device to third parties.
If this manual is lost, please request another one. For the current version, please refer to our webpage
www.eppendorf.com
The Centrifuge 5430/5430 R is available in two versions: key pad or rotary knobs. This operating manual
generally describes how to operate the keypad version but it also applies to the rotary knob version.
(international) or www.eppendorfna.com (North America).
1.2 Danger symbols and danger levels
The safety instructions in this manual appear with the following danger symbols and danger levels:
7
1.2.1 Danger symbols
Biohazard Explosion
Electric shock Crushing
Hazard point Material damage
1.2.2 Danger levels
DANGER Will lead to serious injuries or death.
WARNING May lead to serious injuries or death.
CAUTION May lead to light to moderate injuries.
NOTICE May lead to material damage.
1.3 Symbols used
Example Meaning
You are requested to perform an action.
1.
2.
• List.
Perform these actions in the sequence described.
Press this key to perform the described action.
Text Terms from the display of the device.
References useful information.
Operating instructions
Centrifuge 5430/5430 R
8
English (EN)
1.4 Abbreviations used
MTP
Micro test plate
PCR
Polymerase chain reaction
PTFE
Polytetrafluorethylene
RZB/rcf
Relative centrifugal force – g-force in m/s
rpm
Revolutions per minute – in rpm
2
UV
Ultraviolet radiation
2 Product description
1
2
3
4
5
678
1
2
3
4
5
1
9
6
8
7
2.1 Main illustration
Abb. 2-1: Front and rear view of Centrifuge 5430
Product description
Centrifuge 5430/5430 R
English (EN)
9
Fig. 2-1: Front and rear view of Centrifuge 5430
Abb. 2-2: Front and rear view of Centrifuge 5430 R
Fig. 2-2: Front and rear view of Centrifuge 5430 R
10
Product description
Centrifuge 5430/5430 R
English (EN)
1 Emergency lid release
On both sides of the device (see Emergency
release on p. 57).
2 Centrifuge lid
3 Control panel
Keys and dials (dependent on the device version)
for operating the centrifuge(see p. 28).
4Window
Visual control for rotor stop or option for speed
check via stroboscope.
5Display
Depiction of the current centrifuging parameters
and device settings (see p. 28).
6 Mains connection
Connection socket for the mains cable supplied.
Only 5430: The fuse holder is located beneath
(see Fuses on p. 53).
7 Mains switch
Switch for switching the device on and off.
Switch position 0: The device is switched off.
Switch position I: The device is switched on.
8ID plate
9USB port
Interface for error analysis and software updates
by the Technical Service.
2.2 Delivery package
2.2.1 Centrifuge 5430
Quantity Order no.
(International)
1– – See chapter Ordering Information for corresponding device
1 5301 850.249 022654403 4 A (230 V), 2 pieces
oder 5427 850.341 022654381 8.0 AT UL (120 V/100 V), 2 pieces
1 5416 301.001 022634305 Standard
1– – Mains power cable
1 5427 901.124 5427901124 Languages: EN, DE, FR, ES, IT, PT
1 5427 901.132 5427901132 Languages: DA, FI, EL, NL, SV (230 V devices only)
2.2.2 Centrifuge 5430 R
Quantity Order no.
(International)
1– – See chapter Ordering Information for corresponding device
1 5416 301.001 022634305 Standard
1– – Mains power cable
1 5427 901.124 5427901124 Languages: EN, DE, FR, ES, IT, PT
1 5427 901.132 5427901132 Languages: DA, FI, EL, NL, SV (230 V devices only)
Order no.
(North America)
Order no.
(North America)
Description
Centrifuge 5430
version, equipment and order number
Fuse
Rotor key
Operating manual Centrifuge 5430/5430 R
Description
Centrifuge 5430 R
version, equipment and order number
Rotor key
Operating manual Centrifuge 5430/5430 R
Product description
Centrifuge 5430/5430 R
English (EN)
2.3 Features
The multifaceted Centrifuge 5430 has a capacity of 48 × 2.0 mL and reaches max. 30,130 × g/17,500 min-1.
The versatility is reflected in the available rotor options. You can select from 12 different rotors to
centrifuge the following tubes for your various applications:
• Tubes (0.2 to 5.0 mL)
•PCR strips
• Microtainers
•Spin columns
•Cryo tubes
• Conical tubes (15/50 mL)
•Microplates
•PCR plates
• Deepwell plates (max. height 29 mm)
• Slides (with CombiSlide adapter)
Five program keys for rapid loading and saving of parameters, as well as another 45 program places, a
large display and menu-controlled operation all make it easier to use the centrifuge. The Centrifuge 5430
has been designed based on latest ergonomic studies. This facilitates an intuitive and easy operation.
11
The Centrifuge 5430 is available with two different operator panels: one easy to clean keypad or blue rotary
knobs to quickly set the centrifugation parameter.
The Centrifuge 5430 R has an additional temperature control function for centrifugation between -11°C and
+40°C. The Fast Temp function is used to start a temperature control run without samples in order to
quickly bring the rotor chamber, and rotor, bucket and adapter, to the set temperature. This temperature
control cycle can also be started automatically at specified times using the Fast Temp pro function.
12
Product description
Centrifuge 5430/5430 R
English (EN)
2.4 Rotors
The Centrifuge 5430/5430 R can be operated with the following rotors. Before using tubes, observe the
manufacturer's recommended specifications on resistance to centrifugation (max. g-force).
Rotor
FA-45-48-11
With aerosol-tight
QuickLock rotor lid
Rotor
F-45-48-11
With polypropylene
rotor lid
Max. capacity Max. g-force
(rcf)/speed
(rpm) without
adapter
Acceleration/
deceleration time
(soft): with soft ramp
48 tubes 1.5/2.0 mL.
With adapters:
• 0.2 mL PCR tubes
• 0.4 mL tubes
• 0.5 mL tubes
• 0.6 mL Microtainer
Outer ring:
18,213 × g
Inner ring:
16,048 × g/
12,700 rpm
≤ 20 s/20 s
≤ 61 s/65 s (soft)
48 tubes 1.5/2.0 mL.
With adapters:
• 0.2 mL PCR tubes
• 0.4 mL tubes
• 0.5 mL tubes
• 0.6 mL Microtainer
Outer ring:
18,213 × g
Inner ring:
16,048 × g/
12,700 rpm
≤ 20 s/20 s
≤ 61 s/65 s (soft)
Max.
load per
rotor
(1)
bore
(2)
3.75 g
3.75 g
Notes
• Aerosol-tight
QuickLock rotor lid
(aluminum).
(3)
Rotor
FA-45-30-11
With aerosol-tight
QuickLock rotor lid
Rotor
F-45-30-11
With polypropylene
rotor lid
30 tubes 1.5/2.0 mL.
With adapters:
• 0.2 mL PCR tubes
• 0.4 mL tubes
• 0.5 mL tubes
• 0.6 mL Microtainer
30 tubes 1.5/2.0 mL.
With adapters:
• 0.2 mL PCR tubes
• 0.4 mL tubes
• 0.5 mL tubes
• 0.6 mL Microtainer
20,817 × g/
14,000 rpm
≤ 15 s/15 s
≤ 61 s/65 s (soft)
20,817 × g/
14,000 rpm
≤ 15 s/15 s
≤ 61 s/65 s (soft)
3.75 g
3.75 g
• Aerosol-tight
(3)
QuickLock rotor lid
(aluminum).
•PTFE-coated
(particularly resistant
to chemicals),
marked: coated
•Spin columns
available, better with
rotor FA-45-24-11-kit.
Product description
Centrifuge 5430/5430 R
English (EN)
13
Rotor
FA-45-24-11-Kit
With aerosol-tight
QuickLock rotor lid
Rotor
FA-45-16-17
With aerosol-tight
QuickLock rotor lid
Max. capacity Max. g-force
(rcf)/speed
(rpm) without
adapter
Acceleration/
deceleration time
(soft): with soft ramp
24 spin columns or 1.5/2.0 mL
tubes.
With adapters:
• 0.2 mL PCR tubes
19.090 × g/
13,200 rpm
≤ 15 s/16 s
≤ 78 s/90 s (soft)
• 0.4 mL tubes
• 0.5 mL tubes
• 0.6 mL Microtainer
16 tubes 5.0 mL. 21,191 × g/
14,200 rpm
≤ 20 s/20 s
≤ 61 s/66 s (soft)
Max.
load per
rotor
(1)
bore
(2)
3.75 g
9.5 g
Notes
• Aerosol-tight
(3)
QuickLock rotor lid
(aluminum).
• Uniquely high edge,
for all commercial
spin columns.
Also observe the note
on centrifugation with
open tube lids (see
Fixed-angle rotors on
p. 33)
• Aerosol-tight
(3)
QuickLock rotor lid
(aluminum).
Rotor
S-24-11-AT
With aerosol-tight
QuickLock rotor lid
24 tubes 1.5/2.0 mL. 16,049 × g/
12,700 rpm
This rotor exclusively is to be
used with 1.5/2.0 mL reaction
≤ 13 s/16 s
≤ 61 s/66 s (soft)
vessels. Spin Columns and the
adapters including the
corresponding 0.2 mL, 0.4 mL,
0.5 mL and 0.6 mL vessels
must not be used in this rotor.
3.75 g
• Aerosol-tight
(3)
QuickLock rotor lid
(aluminum).
•The rotor must always
be operated with a
rotor lid.
14
Product description
Centrifuge 5430/5430 R
English (EN)
Rotor
FA-45-24-11-HS
Rotor
F-45-64-5-PCR
Max. capacity Max. g-force
(rcf)/speed
(rpm) without
adapter
Acceleration/
deceleration time
(soft): with soft ramp
24 tubes 1.5/2.0 mL.
With adapters:
• 0.2 mL PCR tubes
• 0.4 mL tubes
30,130 × g/
17,500 rpm
≤ 21 s/16 s
≤ 61 s/65 s (soft)
• 0.5 mL tubes
• 0.6 mL Microtainer
64 PCR tubes (0.2 mL)
or
13,543 × g/
11,800 rpm
eight 5-tube or 8-tube
PCR strips,
each with the enclosed
≤ 12 s/15 s
≤ 62 s/65 s (soft)
adapters.
Max.
load per
rotor
(1)
bore
(2)
3.75 g
3.4 g
(without
adapter)
Notes
• Aerosol-tight
(3)
rotor
lid (aluminum).
• Max. g-force/speed
(30,130 × g/17,500
rpm) only with tubes
approved by the manufacturer for this
speed.
• PTFE-coated (particularly resistant to
chemicals), marked:
coated
• Spin columns available, better with rotor
FA-45-24-11-kit.
•The rotor can only be
tightened and loosened using the special
rotor key for rotor
FA-45-24-11-HS (see
p. 42).
Rotor
F-45-18-17-Cryo
18 Cryo tubes
or
18 sealable centrifugation
tubes,
max. Ø: 16.9 mm.
With supplied adapters: max.
Ø: 13.4 mm, max. tube length:
50 mm.
8,324 × g/
8,900 rpm
≤ 8 s/11 s
≤ 77 s/85 s (soft)
8.7 g • Setting the g-force/
speed in increments
of 10 × g or 10 rpm.
Product description
Fixed-angle
rotor
Aerosol-tight
version
Angle of
bores
Number of
bores
∅ of
bores (mm)
AT
24 11
Aerosol-tight
version
Number of
bores
∅ of
bores (mm)
Swing-bucket
rotor
Centrifuge 5430/5430 R
English (EN)
15
Rotor
F-35-6-30
Rotor
A-2-MTP
Max. capacity Max. g-force
(rcf)/speed
(rpm) without
adapter
Acceleration/
deceleration time
(soft): with soft ramp
6 conical tubes 50 mL, with or
without skirted bottom
or
6 conical tubes 15 mL, with
7,745 × g/
7,830 rpm
≤ 23 s/23 s
≤ 62 s/67 s (soft)
enclosed adapters
or
6 Centriplus centrifuge filter
units with adapters.
Two buckets to hold:
•Microplates
2,204 × g/
4,680 rpm
• Cell culture plates
•PCR plates
• Deepwell plates (max.
≤ 18 s/21 s
≤ 63 s/67 s (soft)
height 29 mm)
• Slide (with CombiSlide
adapter)
Max.
Notes
load per
rotor
(1)
bore
(2)
110 g • The rotor can only be
removed and inserted
using the supplied
removal tool.
•Centrifugation of
round-bottom tubes
and blood taking
systems can be
completed using
additional adapters
(see p. 128).
170 g
(per
bucket)
• PCR plates can only
be centrifuged with
appropriate adapters.
• Max. loading height:
29 mm.
• Only 5430 R: More
efficient cooling via
centrifugation without
wind shield upper
shell (see
Swing-bucket rotor on
p. 34).
(1) Maximum load per rotor bore for adapter + tube + contents.
(2) According to DIN 58 970 (device versions: 230 V, 120 V and 100 V, 50 to 60 Hz).
(3) Aerosol tightness tested and certified by the Centre of Emergency Preparedness and Response, Health
Protection Agency, Porton Down (UK) (see certificates at the end of this operating manual).
For the rotors and rotor lids labeled coated, color fluctuations may occur as a result of the production
process. These fluctuations have no effect on service life or resistance to chemicals.
16
Product description
Centrifuge 5430/5430 R
English (EN)
2.4.1 rcf display and calculation
Use the rpm/rcf key to switch the speed of centrifugation display from rpm to g-force
(rcf). Ensure that the g-force displayed during switching is standardized to suit the
rotor in question without an adapter. When adapters are used, the following maximum
g-forces (rcf) can be achieved at maximum speed:
Rotor Adapter Max.centrifugation
radius r
Rotor FA-45-48-11/
Rotor F-45-48-11
Rotor FA-45-30-11/
Rotor F-45-30-11
Rotor
FA-45-24-11-Kit
Rotor FA-45-16-17 For 5.0 mL micro test tubes 9.4 21,191
Rotor S-24-11-AT Without adapter 8.9 16,049
Rotor
FA-45-24-11-HS
Rotor
F-45-64-5-PCR
Rotor
F-45-18-17-Cryo
Without adapter Outer ring: 10.1
Inner ring: 8.9
For 0.2 mL PCR tubes Outer ring: 8
Inner ring: 6.8
For 0.4 mL tubes Outer ring: 10.1
Inner ring: 8.9
For 0.5 mL tubes Outer ring: 9
Inner ring: 7.8
For 0.6 mL Microtainer Outer ring: 10.1
Inner ring: 8.9
Without adapter 9.5 20,871
For 0.2 mL PCR tubes 7.4 16.215
For 0.4 mL tubes 9.5 20,871
For 0.5 mL tubes 8.4 18,407
For 0.6 mL Microtainer 9.5 20,817
Without adapter 9.8 19,090
For 0.2 mL PCR tubes 7.7 15,000
For 0.4 mL tubes 9.8 19,090
For 0.5 mL tubes 8.7 16,950
For 0.6 mL Microtainer 9.8 19,090
Without adapter 8.8 30,130
For 0.2 mL PCR tubes 6.7 22,940
For 0.4 mL tubes 8.8 30,130
For 0.5 mL tubes 7.7 26,364
For 0.6 mL Microtainer 8.8 30,130
For PCR strips, inside 7.7 11,987
For PCR strips, outside 8.7 13,543
Without adapter 9.4 8,320
For Cryo tubes 9.0 7,970
max
[cm]
Max. g-force (rcf)
Outer ring: 18,210
Inner ring: 16,048
Outer ring: 14,425
Inner ring: 12,261
Outer ring: 18,210
Inner ring: 16,048
Outer ring: 16,229
Inner ring: 14,065
Outer ring: 18,210
Inner ring: 16,048
Product description
Centrifuge 5430/5430 R
English (EN)
17
Rotor Adapter Max.centrifugation
radius r
max
[cm]
Max. g-force (rcf)
Rotor F-35-6-30* For 15 mL conical tubes 11.0 7,540
For 50 mL conical tubes 10.5 7,197
for Centriplus centrifuge filter units 11.1 7,567
A-2-MTP rotor Without adapter 9.0 2,204
for 384 well PCR plates 7.7 1,885
for 96 well PCR plates 7.3 1,788
CombiSlide adapter 7.7 1,885
*) Centrifugation of round-bottom tubes and blood taking systems can be done using additional adapters
(see p. 128).
To determine the g-force (rcf) for a special adapter, you can perform a calculation according to DIN 58 970
with the following formula:
-5
rcf = 1.118 · 10
· n2 · r
max
n: speed in min-1 (rpm)
: max. centrifuging radius in cm
r
max
Example:
In Rotor FA-45-30-11, the 0.5 mL adapter has a maximum radius of 8.4 cm. At 7,000 rpm, a maximum
g-force of 4,600 × g is achieved.
18
Safety
Centrifuge 5430/5430 R
English (EN)
3Safety
3.1 Intended use
The Centrifuge 5430/5430 R is intended exclusively for indoor use and for separating aqueous solutions
and suspensions of various densities in approved test tubes.
3.2 User profile
This device may only be operated by trained specialist staff. They must have carefully read the operating
manual and be familiar with the function of the device.
3.3 Application limits
3.3.1 Declaration concerning the ATEX directive (94/9/EC)
DANGER! Risk of explosion.
Do not operate the device in areas where work is completed with explosive substances.
Do not use this device to process any explosive or highly reactive substances.
Do not use this device to process any substances which may generate an explosive
atmosphere.
Due to its design and the environmental conditions inside the device, the Centrifuge 5430/5430 R is not
suitable for use in a potentially explosive atmosphere.
The device only must be used in a safe environment, such as the open environment of a ventilated
laboratory or fume hood. The use of substances which could create a potentially explosive atmosphere is
not permitted. The final decision on the risks associated with the use of these types of substances is the
responsibility of the user.
3.3.2 Maximum service life for accessories
WARNING! Risk of injury from chemically or mechanically damaged accessories.
Even minor scratches and cracks can lead to serious internal material damage.
Protect all accessory parts from mechanical damage.
Inspect the accessories for damage before each use. Replace any damaged accessories.
Do not use rotors, rotor lids or buckets with signs of corrosion or mechanical damage (e.g.,
deformations).
Do not use any accessories whose maximum service life has been exceeded.
When inserting the buckets and rotors, ensure that they do not become scratched.
CAUTION! Risk of injury due to chemically damaged rotor lids or caps.
Transparent rotor lids or caps made from PC, PP or PEI may lose their strength under the
impact of organic solvents (e.g., phenol, chloroform).
If rotor lids or caps have come into contact with organic solvents, they should be cleaned
immediately.
Regularly check the rotor lids and caps for damages and cracks.
Immediately replace rotor lids or caps that have cracks or milky stains.
Safety
Centrifuge 5430/5430 R
English (EN)
The rotors listed below, and the corresponding buckets and rotor lids, have a maximum service life of the
number of years or cycles listed in the table.
Since 2012, Eppendorf has been stating the maximum service life of the rotors and accessories both in
years and in the maximum number of cycles. The decisive factor for the service life is which case occurs
first, generally this is the number of years in operation.
Rotor Maximum service from commissioning onward
FA-45-48-11
(With QuickLock)
F-45-48-11
(With rotor lid thread)
FA-45-30-11
(With QuickLock)
FA-45-24-11-Kit
(With QuickLock)
FA-45-16-17
(With QuickLock)
S-24-11-AT
(With QuickLock)
A-2-MTP
With the corresponding bucket and upper shell
of the wind shield
QuickLock rotor lid - 3 years
Rotor lid and caps made of polycarbonate (PC),
polypropylene (PP) or polyetherimide (PEI)
Plastic adapters - 1 year
100,000 mechanical cycles 10 years
100,000 mechanical cycles 10 years
100,000 mechanical cycles 10 years
100,000 mechanical cycles 10 years
100,000 mechanical cycles 10 years
100,000 mechanical cycles 10 years
100,000 mechanical cycles 7 years
-3 years
19
For all other rotors and rotor lids of this centrifuge there is no service life limit as long as the following
requirements are met:
•Proper use,
• Recommended maintenance
• Undamaged condition
The date of manufacture is stamped on the rotors in the format 03/10 (= March 2010) or on the inside of the
plastic rotor lids in the form of a clock .
To ensure aerosol tightness, the following applies:
• Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.
• Replace the seal of QuickLock rotor lids after 50 autoclaving cycles.
20
Safety
Centrifuge 5430/5430 R
English (EN)
3.4 Information on product liability
In the following cases, the designated protection of the device may be compromised. Liability for any
resulting property damage or personal injury is then transferred to the operator:
• The device is not used in accordance with the operating manual.
• The device is used outside of its intended use.
• The device is used with accessories or consumables which are not recommended by Eppendorf.
• The device is maintained or repaired by people not authorized by Eppendorf.
• The user makes unauthorized changes to the device.
3.5 Warnings for intended use
Read the operating manual and observe the following general safety instructions before using the
Centrifuge 5430/5430 R.
3.5.1 Personal injury or damage to the equipment
WARNING! Electric shock due to damage to device or mains cable.
Only switch on the device if the device and mains cable are undamaged.
Only use devices that have been properly installed or repaired.
In case of danger, disconnect the device from the mains supply by pulling the power plug
from the device or the mains socket or, by using the isolating device intended for this
purpose (e.g. emergency stop switch in the laboratory).
WARNING! Lethal voltages inside the device.
Ensure that the housing is always closed and undamaged so that no parts inside the device
can be contacted by accident.
Do not remove the housing of the device.
Do not allow any liquids to penetrate the inside of the housing.
Do not allow the device to be opened by anyone except service personnel who have been
specifically authorized by Eppendorf.
WARNING! Risk from incorrect supply voltage
Only connect the device to voltage sources which correspond to the electrical
requirements on the name plate.
Only use sockets with a protective earth (PE) conductor and suitable power cable.
WARNING! Damage to health due to infectious liquids and pathogenic germs.
When handling infectious liquids and pathogenic germs, observe the national regulations,
the biological security level of your laboratory, the material safety data sheets, and the
manufacturer's application notes.
Use aerosol tight sealing systems for the centrifugation of these substances.
When working with pathogenic germs belonging to a higher risk group, more than one
aerosol-tight bioseal must be used.
Wear personal protective equipment.
For full instructions regarding the handling of germs or biological material of risk group II
or higher, please refer to the "Laboratory Biosafety Manual" (Source: World Health
Organization, current edition of the Laboratory Biosafety Manual).
Safety
Centrifuge 5430/5430 R
English (EN)
WARNING! Risk of injury when opening or closing the centrifuge lid.
There is a risk of crushing your fingers when opening or closing the centrifuge lid.
When opening or closing the centrifuge lid, do not reach between the lid and device or into
the latching mechanism of the lid.
Always open the centrifuge lid completely to prevent it from falling.
CAUTION! Poor safety due to incorrect accessories and spare parts.
The use of accessories and spare parts other than those recommended by Eppendorf may
impair the safety, functioning and precision of the device. Eppendorf cannot be held liable or
accept any liability for damage resulting from the use of incorrect or non-recommended
accessories and spare parts, or from the improper use of such equipment.
Only use accessories and original spare parts recommended by Eppendorf.
NOTICE! Damage to device due to spilled liquids.
1. Switch off the device.
2. Disconnect the device from the power supply.
3. Carefully clean the device and the accessories in accordance with the cleaning and
disinfection instructions in the operating manual.
4. If a different cleaning and disinfecting method is to be used, contact Eppendorf AG to
ensure that the intended method will not damage the device.
21
NOTICE! Damage to electronic components due to condensation.
Condensate can form in the device after it has been moved from a cool environment to a
warmer environment.
After installing the device, wait for at least 4 h. Only then connect the device to the mains
power supply.
3.5.2 Incorrect handling of the centrifuge
NOTICE! Damage from knocking against or moving the device during operation.
If the rotor bangs against the rotor chamber wall, it will cause considerable damage to the
device and rotor.
Do not move or knock against the device during operation.
22
Safety
Centrifuge 5430/5430 R
English (EN)
3.5.3 Incorrect handling of the rotors
WARNING! Risk of injury from improperly attached rotors and rotor lids.
Only centrifuge with rotor and rotor lid firmly tightened.
If unusual noises occur when the centrifuge starts, the rotor or the rotor lid may not be
properly secured. Immediately press the start/stop key to stop centrifuging.
CAUTION! Risk of injury due to asymmetric loading of a rotor.
Load rotors symmetrically with identical tubes or plates and buckets.
Only load adapters with suitable tubes or plates.
Always use tubes or plates of the same type (weight, material/density and volume).
Check that loading is symmetrical by balancing the adapters and tubes or plates used with
scales.
The device automatically detects imbalances during operation and stops the run immediately
with an error message and a signal tone. Check the loading, balance the tubes and re-start the
centrifugation.
CAUTION! Risk of injury from overloaded rotor.
The Centrifuge 5430/5430 R is designed for the centrifugation of material with a max. density
of 1.2 g/mL at maximum speed and filling volume and/or load.
Do not exceed the maximum load of the rotor.
NOTICE! Damage to rotors from aggressive chemicals.
Rotors are high-quality components which withstand extreme stresses. This stability can be
impaired by aggressive chemicals.
Avoid using aggressive chemicals, including strong and weak alkalis, strong acids,
solutions with mercury, copper and other heavy metal ions, halogenated hydrocarbons,
concentrated saline solutions and phenol.
If the rotor is contaminated by aggressive chemicals, clean it immediately using a neutral
cleaning agent. This applies to the rotor bores, in particular.
Due to the manufacturing process, color variations may occur on rotors marked "coated".
These color variations do not effect service life or resistance to chemicals.
NOTICE! If handled incorrectly, the rotor can fall over.
The rotor can fall over if the buckets are used as a handle.
Remove the buckets before inserting and/or removing a swing-bucket rotor.
Always use both hands to carry the rotor cross.
CAUTION! Risk of injury due to chemically damaged rotor lids or caps.
Transparent rotor lids or caps made from PC, PP or PEI may lose their strength under the
impact of organic solvents (e.g., phenol, chloroform).
If rotor lids or caps have come into contact with organic solvents, they should be cleaned
immediately.
Regularly check the rotor lids and caps for damages and cracks.
Immediately replace rotor lids or caps that have cracks or milky stains.
3.5.4 Extreme strain on the centrifuging tubes
CAUTION! Risk of injury from overloaded tubes.
Note the loading limits specified by the tube manufacturer.
Only use tubes which are approved by the manufacturer for the required rcf.
NOTICE! Risk from damaged tubes.
Damaged tubes must not be used, as this could cause further damage to the device and the
accessories and loss of the samples.
Before use, visually check all of the tubes for damage.
NOTICE! Risk from open tube lids.
Open tube lids can brake off during centrifugation and damage the rotor and the centrifuge.
Carefully seal all tube lids before centrifuging.
Exception: Note the information on the centrifugation of spin columns in the rotor
FA-45-24-11-Kit (see p. 34).
Safety
Centrifuge 5430/5430 R
English (EN)
23
NOTICE! Hazard to plastic tubes from organic solvents.
The density of plastic tubes is reduced when organic solvents (e.g., phenol, chloroform) are
used, i.e. the tubes could become damaged.
Observe the manufacturer's specifications for chemical resistance of the tubes.
NOTICE! Sample tubes heat up.
In uncooled centrifuges, the temperature in the rotor chamber, rotor and sample can increase
to above 40 °C, based on the run time, g-force (rcf)/speed and ambient temperature.
Note that this can reduce the centrifugation resistance of the sample tubes.
Please note the temperature resistance of the samples.
24
Safety
Centrifuge 5430/5430 R
English (EN)
3.5.5 Aerosol-tight centrifugation
WARNING! Risk to health due to limited aerosol tightness with incorrect rotor/rotor lid
combination.
Aerosol-tight centrifugation is guaranteed only if the rotors and rotor lids intended for this
purpose are used. The designation of aerosol-tight fixed-angle rotors always starts with
FA.The aerosol-tight rotors and rotor lids of this centrifuge are additionally marked with a red
ring on the rotor and a red rotor lid screw.
Aerosol-tight swing-bucket rotors are marked with AT (aerosol-tight).
For aerosol-tight centrifugation, always simultaneously use rotors and rotor lids which are
marked as aerosol-tight in the centrifuge intended for the corresponding purpose. The
details specifying in which centrifuge you may use the aerosol-tight rotors and rotor lids
can be found on the rotor and, beginning from production date of October 2003, on the
upper side of the rotor lid.
Only use aerosol-tight rotor lids in combination with rotors which are marked on the rotor
lid.
Only use aerosol-tight buckets with the corresponding caps.
WARNING! Damage to health as a result of limited aerosol tightness and incorrect usage.
Autoclaving, mechanical stresses and contamination by chemicals or other aggressive
solvents can impair the aerosol-tightness of the rotors and rotor lids.
Check the integrity of the seals of the aerosol-tight rotor lids or caps before each use.
Only use aerosol-tight rotor lids or caps if the seals are undamaged and clean.
Lightly grease the threads of the rotor lid screw with pivot grease after every proper
autoclaving (121 °C, 20 min.) (int. order no. Int. 5810 350.050, North America
022634330).
Replace aerosol-tight rotor lids and caps after 50 autoclaving cycles.
For QuickLock rotor lids, the seal must be replaced after 50 autoclaving cycles.
Never store aerosol-tight rotors or buckets closed.
3.6 Safety notes on the device
Symbol Meaning Location
Hazard point. 5430: Rear of the device
CAUTION
Always tighten the rotor with the enclosed rotor
key.
CAUTION
Close all tubes and use a rotor lid.
5430 R: Right side of the
device
Upper side of device, under
the centrifuge lid.
Upper side of device, under
the centrifuge lid.
4 Installation
4.1 Selecting the location
NOTICE! If an error occurs, the objects in the immediate proximity of the device will be
damaged.
In accordance with recommendations in EN 61010-2-020, leave a safety clearance of
30 cm around the device during operation.
Please remove all materials and objects from this area.
NOTICE! Damage from overheating.
Do not install the device near heat sources (e.g. heating, drying cabinet).
Do not expose the device to direct sunlight.
Ensure unobstructed air circulation. Maintain a clearance of at least 30 cm around all
ventilation grilles.
Installation
Centrifuge 5430/5430 R
English (EN)
25
Select the location for the device according to the following criteria:
• Suitable power connection as per the name plate (230 V/120 V/100 V).
• Stable, horizontal and resonance-free lab bench.
• A well ventilated environment which is protected from direct sunlight to prevent the device from heating
up more.
4.2 Preparing installation
Prerequisites
The weight of the centrifuge is 29 kg (5430) or 56 kg (5430 R). A second person is needed to unpack and
position the device.
Retain the packaging material and the transport protection device for subsequent transport or
storage. See also the instructions relating to transport (see p. 58).
Perform the following steps in the sequence described.
Centrifuge 5430 Centrifuge 5430 R
1. Open the packaging board.
2. Remove the covering cardboard.
3. Remove the accessories.
4. Grip from the strap retainers and have two
persons lift the centrifuge out of the box.
5. Pull off the strap retainers, do not cut.
6. Remove the front and rear transport securing
devices from the centrifuge.
7. Remove the plastic sleeve.
8. Carefully lift the centrifuge on one side and pull
off the transport securing device of the motor
on the underside of the centrifuge.
1. Open the packaging board.
2. Remove the accessories.
3. Lift and remove the front and rear transport
securing devices.
4. Grip from the fabric straps and have two people
lift the centrifuge out of the box.
5. Pull off the textile straps, do not cut.
6. Remove the plastic sleeve.
26
Installation
Centrifuge 5430/5430 R
English (EN)
4.3 Installing the instrument
Prerequisites
The device is on a suitable lab bench.
NOTICE! Damage to electronic components due to condensation.
Condensate can form in the device after it has been moved from a cool environment to a
warmer environment.
After installing the device, wait for at least 4 h. Only then connect the device to the mains
power supply.
NOTICE! Centrifuge 5430 R: compressor damage after improper transport.
Only switch on the centrifuge 4 h after installation.
Perform the following steps in the sequence described.
1. Let the device warm up to ambient temperature for at least 4 hours to avoid damaging electronic
assemblies as a result of condensation and avoid damaging the compressor (only 5430 R).
2. Check that the mains voltage and frequency match the requirements on the device type plate.
3. Connect the centrifuge to the mains and switch it on using the mains/power switch on the rear of the
device (Centrifuge 5430) or at the right side of the device (Centrifuge 5430 R).
• The standby key lights green.
•The display is active.
• Only 5430: Lid opens automatically
4. Only 5430: Remove the transport securing device of the motor shaft.
5. If the scope of delivery includes a rotor, dismantle and remove it using the supplied rotor key.
6. Only 5430: Remove the transport securing device of the air guide ring.
Centrifuge 5430/5430 R
7. Use the details included in the scope of delivery to check that the delivery is complete.
8. Check all parts for any transport damage. Contact your dealer if any damage is found.
Retain the packaging material and the transport protection device for subsequent transport or
storage. See also the instructions relating to transport (see p. 58).
Installation
27
English (EN)
28
1
2
3
4
5
6
7891011
13
12
Operation
Centrifuge 5430/5430 R
English (EN)
5 Operation
5.1 Overview of operating controls
The Centrifuge 5430/5430 R is available in two versions: keypad or rotary knobs. This operating manual
generally describes how to operate the keypad version. However, it also applies to the rotary knob version.
Before using the Centrifuge 5430/5430 R for the first time, familiarize yourself with the operating controls
and the display.
Abb. 5-1: Control panel and display of Centrifuge 5430/5430 R (keypad version).
Fig. 5-1: Control panel and display of Centrifuge 5430/5430 R (keypad version).
1 Select program
Press briefly: load the stored centrifuging
parameters.
Press and hold (> 2 sec): save the current
centrifuging parameters (see p. 47).
2 Short Spin centrifugation
(see p. 40)
3Release lid
4 Start and stop centrifugation
5 Activate/deactivate standby mode
Key lights green: centrifuge is ready for
operation.
Key lights red: standby mode active (see p. 41).
6Display
8 Adjust the temperature (only 5430 R)
9 Adjust the centrifuging duration
Dependent on device version, designed as key or
rotary knob.
10 Start the temperature control cycle Fast Temp
(only 5430 R)
(see p. 36)
11 Switch the displayed centrifuging speed (rpm/
rcf)
12 Call and select the menu parameters
(see p. 47)
13 Navigating the menu
(see p. 29)
7 Set speed of centrifugation
Dependent on device version, designed as key or
rotary knob.
Abb. 5-2: Display of the Ce ntrifuge 5430/5430 R
1
2 3 4 5 7
6
8
10
9
111213
Fig. 5-2: Display of the Centrifuge 5430/5430 R
Operation
Centrifuge 5430/5430 R
English (EN)
29
1 Program number (if enabled)
2 Status of the function At set rpm
: Start of operation when reaching 95% of the
preset g-force (rcf) or speed.
: Start of run time immediately.
3 Status of the key lock
: Centrifuging parameters cannot be modified
unintentionally.
: No key lock.
4 Status of the loudspeaker
: Switched on.
: Switched off.
5 Status of the centrifuge
: Centrifuge lid unlocked.
: Centrifuge lid locked.
(flashing): Centrifuging in progress.
6 Temperature control cycle programming (only
5430 R)
: Fast Temp pro is enabled, i.e., start time
and temperature of the temperature control run
have been programmed (see p. 37).
8Soft ramp
: Rotor accelerates and brakes slowly.
No symbol: Rotor accelerates and brakes rapidly.
9Standard display
10 Extended display (if enabled)
11 g-force/speed
12 Temperature (only 5430 R)
13 Centrifuging duration
7Time
Please also read the precise description of the individual functions (see p. 47).
5.2 Menu navigation
The menu consists of two levels. To change settings, generally proceed as follows.
1. Open menu.
30
Operation
Centrifuge 5430/5430 R
English (EN)
2. Select the desired menu item.
3. Confirm selection.
4. Select the setting of the parameters in question.
5.
6.
Confirm changed setting.
A tick appears in front of the confirmed setting.
Keep pressing the key until you reach the desired menu level or exit the menu.
Some menus can only be exited by selecting and confirming the menu item Back /
Zurück / Retour / Atrás.
5.3 Configure centrifuge
5.3.1 Set menu language
Proceed as follows to set menu language.
1. Open the menu.
2. Select Settings.
3. Confirm your selection.
4. Select Language.
5. Confirm your selection.
6. Select English, Deutsch, Francais or Espanol.
7.
8. Press key several times to exit the menu.
Confirm your selection. A tick appears in front of the selected language. The setting
takes effect immediately.
5.3.2 Setting the date and time
Proceed as follows to set date and time.
1. Open the menu.
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