Operation Manual
Hydrolysis Unit
B�411/E�416
96685
en
Table of contents
Table of contents
1 About this manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
1.1 Abbreviations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2 Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.1 User qualification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.2 Proper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.3 Improper use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
2.4 Warning notices used in this manual . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5 Product safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5.1 Instrument-related hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5.2 Safety measures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
2.5.3 Safety elements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.6 General safety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3 Technical data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1 Scope of delivery . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1.1 Standard instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.1.2 Standard accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
3.1.3 Optional accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
3.2 Technical data overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
3.3 Materials used. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
4 Description of function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1 Overview over the instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2 Description of the operating elements . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.3 Principle of hydrolysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
5 Putting into operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.1 Installation site. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.2 Electrical connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
5.3 Connecting the suction hose to the suction tube and the pump. . . . . . . . . . . . 15
5.4 Installing the rubber couplings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
5.5 Filtration positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Read this manual carefully before installing and running your system and note the safety precautions
in chapter 2 in particular. Store the manual in the immediate vicinity of the instrument, so that it can be
consulted at any time.
No technical modifications may be made to the instrument without the prior written agreement of
Buchi. Unauthorized modifications may affect the system safety or result in accidents.
This manual is copyright. Information from it may not be reproduced, distributed, or used for competitive purposes, nor made available to third parties. The manufacture of any component with the aid of
this manual without prior written agreement is also prohibited.
If you need another language version of this manual, you can download it at
www.buchi.com.
3 B-411/E-416 Operation Manual, Version E
Table of contents
6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6.1 Sample weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6.2 Preheating. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6.3 Preparing the digestion vessels . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
6.4 Preparing the glass sample tubes . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
6.5 Choosing the heat output . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.6 Hydrolysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.7 Filtration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
6.8 Drying the hydrolyzed samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.9 Preparing the extraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6.10 Extraction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
7 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
7.1 Housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
7.2 Heating chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
7.3 Glass parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.4 Rubber couplings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.5 Functional test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.6 Main fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
7.7 Customer service . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
8 Shutdown, storage, transport and disposal . . . . . . . . . . . . . . . . . . . . . . . . 24
8.1 Storage and transport . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
8.2 Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
9 Spare parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
10 Declarations and requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
10.1 FCC requirements (for USA and Canada) . . . . . . . . . . . . . . . . . . . . . . . 26
10.2 Declaration of conformity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
4
B-411/E-416 Operation Manual, Version E
1 About this manual
This manual describes the Hydrolysis Unit B-411/E-416 and provides all information required for its
safe operation and to maintain it in good working order.
It is addressed to laboratory personnel in particular.
NOTE
The symbols pertaining to safety (WARNINGS and ATTENTIONS) are explained in chapter 2.
1.1 Abbreviations
EPDM: Ethylene Propylene Dimonomer
PPO: Polyphenylene Oxide
1 About this manual
5 B-411/E-416 Operation Manual, Version E
2 Safety
This chapter points out the safety concept of the instrument and contains general rules of behavior
and warnings from hazards concerning the use of the product.
The safety of users and personnel can only be ensured if these safety instructions and the safetyrelated warnings in the individual chapters are strictly observed and followed. Therefore, the manual
must always be available to all persons performing the tasks described herein.
2.1 User qualification
The instrument may only be used by laboratory personnel and other persons who on account of
training or professional experience have an overview of the dangers which can develop when
operating the instrument.
Personnel without this training or persons who are currently being trained require careful instruction.
The present Operation Manual serves as the basis for this.
2 Safety
2.2 Proper use
The instrument has been designed and built for laboratories. It serves for the hydrolysis of products for
fat determination with the use of diluted hydrochloric acid.
2.3 Improper use
Applications not mentioned above are improper. Also, applications, which do not comply with the
technical data, are considered improper.
The operator bears the sole risk for any damages caused by such improper use.
The following uses are expressly forbidden:
Use of the instrument in rooms which require ex-protected instruments.•
Use on samples, which can explode or inflame (example: explosives, etc.) due to shock, friction, •
heat or spark formation.
Use in overpressure situations.•
6 B-411/E-416 Operation Manual, Version E
2.4 Warning notices used in this manual
WARNING
Generally, the triangular warning symbol indicates the possibility of personal injury or even loss of life
if the instructions are not followed.
WARNING
Hot surface.
WARNING
Electrical hazard.
ATTENTION
With the general “Read this” symbol, ATTENTIONs indicate the possibility of equipment damage,
malfunctions or incorrect process results, if instructions are not followed.
NOTE
Useful tips for the easy operation of the instrument.
2 Safety
2.5 Product safety
The Hydrolysis Unit is designed and built in accordance with current state-of-the-art technology.
Nevertheless, risks to users, property, and the environment can arise when the instrument is used
carelessly or improperly.
The manufacturer has determined residual dangers emanating from the instrument
if the instrument is operated by insufficiently trained personnel.•
if the instrument is not operated according to its proper use.•
Appropriate warnings in this manual serve to make the user alert to these residual dangers.
2.5.1 Instrument-related hazards
Pay attention to the following safety notices:
WARNING
Potentially hot surfaces during operation, especially at the glass parts or due to hot hydrochloric acid.
Always be aware of the risk of being burned.•
WARNING
Potential implosion risk if used with damaged glassware.
Beware of damaged or cracked glass parts.•
2.5.2 Safety measures
Always wear personal protective equipment such as protective eye goggles, protective clothing and
gloves when working with the instrument.
7 B-411/E-416 Operation Manual, Version E
2.5.3 Safety elements
The main switch of the instrument serves as safety switch. In case of overload or short circuit it •
automatically falls back to the zero position.
2.6 General safety rules
Responsibility of the operator
The head of laboratory is responsible for training his personnel.
The operator shall inform the manufacturer without delay of any safety-related incidents which might
occur during operation of the instrument. Legal regulations, such as local, state and federal laws
applying to the instrument must be strictly followed.
Duty of maintenance and care
The operator is responsible for ensuring that the instrument is operated in proper condition only, and
that maintenance, service, and repair jobs are performed with care and on schedule, and by authorized personnel only.
2 Safety
Spare parts to be used
Use only genuine consumables and genuine spare parts for maintenance to assure good system
performance and reliability. Any modifications to the spare parts used are only allowed with the prior
written permission of the manufacturer.
Modifications
Modifications to the instrument are only permitted after prior consultation with and with the written
approval of the manufacturer. Modifications and upgrades shall only be carried out by an authorized
Buchi technical engineer. The manufacturer will decline any claim resulting from unauthorized modifications.
8 B-411/E-416 Operation Manual, Version E
3 Technical data
This chapter introduces the reader to the instrument specifications. It contains the scope of delivery,
technical data, requirements and performance data.
3.1 Scope of delivery
Check the scope of delivery according to the order number.
NOTE
For detailed information on the listed products, see www.buchi.com or contact your local dealer.
3.1.1 Standard instrument
3 Technical data
Table 3-1: Standard instrument
Product Order number
B-411 Hydrolysis Unit, 4 positions,
230 V, 50/60 Hz
B-411 Hydrolysis Unit, 4 positions,
120 V, 50/60 Hz
B-411 Hydrolysis Unit, 4 positions, Viton,
230 V, 50/60 Hz
E-416 Hydrolysis Unit, 6 positions,
230 V, 50/60 Hz
E-416 Hydrolysis Unit, 6 positions,
120 V, 50/60 Hz
37455
37456
37461
42870
42871
9 B-411/E-416 Operation Manual, Version E
3.1.2 Standard accessories
3 Technical data
Table 3-2: Standard accessories
5
3
B-411 E-416 Product Order number
1 2 a Set of 4 digestion vessels, 300 ml 37377
4 6 b Sample aspiration tubes 37380
6
2
4
1
-
c Set of 4 caps for rinsing digestion
-
6
vessel
Cap for rinsing digestion vessel
37463
19741
1
1 2 d Set of 4 stoppers, Ø 45 mm 37725
1 1 e Inbus wrench 3.0 mm 00610
4 6 f Rubber coupling, EPDM 37381
4 - Rubber coupling, Viton 44422
1 1 Bottle of 2.5 kg quartz sand
37689
(0.3 - 0.9 mm)
1 1 Suction tube for B-411 37387
1 1 Suction tube for E-416 42868
10 B-411/E-416 Operation Manual, Version E
a
3 Technical data
Table 3-2: Standard accessories (cont.)
B-411 E-416 Product Order number
1 1 a Power cable
Type CH 10010
Type Schuko / Japan 10016
Type GB 17835
Type USA 33763
Type AUS 17836
1 1 Operation Manual:
English 96685
German 96684
French 96686
Italian 96687
Spanish 96688
3.1.3 Optional accessories
5
3
2
Product Order number
Packaging, complete 36055
a Holder for 4 digestion vessels 02013
Holder for 6 digestion vessels 43039
Table 3-3: Optional accessories
4
1
Holder for 12 digestion vessels 43041
b Holder for glass sample tubes, 4 posi-
tions, not microwavable
Holder for glass sample tubes,
6 positions, microwavable
37462
49424
c Set of 4 glass sample tubes for B-811 37281
Set of 2 glass sample tubes for E-816 49430
d Set of 4 digestion vessels, 300 ml 37377
e Water jet pump, plastic 02913
f Vacuum hose, Ø 6 x 16 mm 17622
g Pair of glass tongs 02004
6
g
11 B-411/E-416 Operation Manual, Version E
3.2 Technical data overview
Table 3-4: Technical data
Dimensions (W x H x D) 275 x 600 x 570 mm
Weight B-411: 13.5 kg
Power system voltage / frequency 230 V ± 10 % / 50 Hz
Ambient temperature For indoor use only, altitude up to 2000 m above sea
Overvoltage category II
Pollution degree 2
Power consumption 1100 W
3 Technical data
Hydrolysis Unit B-411/E-416
E-416: 14.5 kg
140 W
120 V ± 10 % / 60 Hz
level, maximum relative humidity 80 % for temperatures
up to 30 °C, 10 - 40 °C
100 W
3.3 Materials used
Table 3-5: Materials used
Component Material designation Material code
Glass Borosilicate glass 3.3
Housing Stainless steel 1.4301
Housing cover Plastic PPO + Glass fiber
Insulation plate heating Alkaline earth-silicate fiber
Rubber coupling EPDM
12 B-411/E-416 Operation Manual, Version E
4 Description of function
This chapter explains the basic principle of the instrument, shows how it is structured and gives a
functional description of the assemblies.
For Weibull-Stoldt fat determination in food and animal feed, the product must be hydrolyzed to make
the fat accessible for extraction.
Thus a reproducible fat determination is possible.
The hydrolysis also frees fat-like substances which are mechanically surrounded by different constituents (carbohydrates and protein substances in undamaged cells or starch membranes).
This is also true for colloid-disperse fractions (protein) which, as a result of surface forces, coat the fat
drops (milk, cream, cheese) and for certain fat fractions which are bound, chemically or adsorptively to
other components (phosphatide-protein complexes in yeast, eggs, germs, etc.).
4.1 Overview over the instrument
4 Description of function
a Operation panel
4
3
5
6
2
7
1
b Suction tube
c Glass sample tube
d Sample aspiration tube
e Lift device
f Digestion vessel
g Upper insulation plate
Fig. 4.1: Instrument overview
13 B-411/E-416 Operation Manual, Version E
4.2 Description of the operating elements
1
Fig. 4.2: Operation panel B-411
4.3 Principle of hydrolysis
The sample is boiled in diluted hydrochloric acid to break down
proteins and high-molecular carbohydrates into acid-soluble constituents.
4 Description of function
a Main/safety switch
b Energy regulator
c Heating indicator lamp
3
2
Fig. 4.3: Boil position
Fig. 4.4: Filter position
Afterwards the sample is filtered over a prepared glass sample tube
and dried. The celite distributes the hydrolysate evenly in the tube,
prevents clumping and facilitates the extraction to be carried out.
The fat can now be extracted with an appropriate solvent.
14 B-411/E-416 Operation Manual, Version E
5 Putting into operation
This chapter describes how the instrument is installed and gives instructions on initial startup.
NOTE
Inspect the instrument for damages during unpacking. If necessary, prepare a status report immediately to inform the postal company, railway company or transportation company.
Remove the transportation lock at the bottom of the instrument.
Keep the original packaging for future transportation.
5.1 Installation site
Install the instrument on a clean, flat and stable base.
ATTENTION
To ensure the instrument safety do not place any object on top of it. Provide a distance of at least
30 cm between the instrument and another object or the wall. There should be no containers, chemicals or other devices located behind the instrument.
5 Putting into operation
We recommend to operate the instrument under a ventilation hood. Do not place the Extraction Unit
under the same hood.
5.2 Electrical connections
WARNING
Make sure that the voltage on the socket corresponds to the voltage given on the type plate of the
instrument.
Always connect the instrument to an earthed socket. External connections and extension cables
must be provided with an earthed conductor lead (3-pole couplings, cable or plug equipment) as the
mains lead has a molded plug, thus avoiding risks due to inadvertent defective wiring.
Make sure that no electric sparks form in the instrument or its surroundings as they might damage
the instrument.
Connect the Hydrolysis Unit B-411/E-416 to the mains with the power cord contained in the scope of
delivery.
5.3 Connecting the suction hose to the suction tube and the pump
The suction hose is connected to the hose connection (Ø 10 mm) on the suction tube. The fumes
which result from hydrolysis and the wash water resulting from filtering the sample are extracted with
the pump. The suction tube can be connected to a water jet pump or to a vacuum pump with an
intermediate vessel. In order to achieve efficient filtration, the used pump should have a good suction
power and should be resistant to the chemicals used.
15 B-411/E-416 Operation Manual, Version E
5.4 Installing the rubber couplings
The rubber couplings can be carefully installed on the suction tube
and the sample aspiration tube after having moistened them with
water. During the installation, make sure that the collar of the glass
part abuts on the receptacle of the rubber part. The receptacles for
the glass sample tubes are provided with an antistick coating, so
that they can be easily installed and disassembled.
In order to avoid broken glass during the installation of the rubber
couplings, the glass part must be held as near as possible to the
receptacle collar.
Fig. 5.1: Suction tube with rubber coupling
5.5 Filtration positioning
The distance between the sample aspiration tube and the digestion
vessel must be approx. 5 mm.
In order to guarantee optimal filtration, it is necessary to correctly
position the cap.
5 Putting into operation
Fig. 5.2: Sample aspiration tube with digestion vessel
16 B-411/E-416 Operation Manual, Version E
6 Operation
This chapter gives examples of typical instrument applications and instructions on how to operate the
instrument properly and safely.
WARNING
Risk of injury.
Never operate the instrument when glassware is damaged.•
ATTENTION
Hot, caustic hydrochloric acid is used for the operation of the instrument. For this reason, personal
protective equipment must be worn at all times when using this instrument or working in its immediate proximity.
6.1 Sample weight
6 Operation
The sample weight depends on the fat content of the sample. It should be chosen in a way that the
final weight is between 0.5 and 1.5 g of fat. The maximum sample weigth is 10 g.
6.2 Preheating
The “preheating” position serves to preheat the instrument. This preheating procedure takes about
10 minutes. Thus an initial boiling point of the sample is achieved in a shorter period of time. The
preheating must always be carried out before a hydrolysis process is started.
6.3 Preparing the digestion vessels
Put 5 g celite 545 into the digestion vessel.
Fig. 6.1: Adding celite
Weigh-in sample.
Fig. 6.2: Weighing in sample
17 B-411/E-416 Operation Manual, Version E
Fig. 6.3: Adding HCI and mixing
6 Operation
Add 50 ml HCl, 4 mol / l and shake the tube to mix the sample with
the celite and the HCl.
Add another 50 ml HCl, 4 mol / l by pouring it along the side walls
of the digestion vessel to remove probable residues from the tube
wall.
In case of liquid samples, the acid concentration should be adjusted
to the water content of the sample.
Fig. 6.4: Adding HCl to rinse glass wall
6.4 Preparing the glass sample tubes
Evenly distribute approx. 50 g quartz sand (grain size 0.3 - 0.9 mm).
The grain size is important, as in case it is too small, it will clog the
glass sample tube and in case it is too large, the celite will pass the
glass sample tube during the filtration step.
Fig. 6.5: Adding quartz sand
Add a layer of 5 g celite 545 in the glass sample tube.
Fig. 6.6: Adding celite
18 B-411/E-416 Operation Manual, Version E
6.5 Choosing the heat output
When the preheating procedure is finished, the hydrolysis can be started. For this purpose we recommend to switch the energy regulator to position 2 for four samples and to position 3 for six samples.
Depending on the boiling behavior, less heat can be provided when setting the regulator to position 1
or more heat can be provided by setting it to position 3.
6.6 Hydrolysis
To carry out a hydrolysis proceed as follows:
Reduce the heat output (position 2 - 3)•
Insert the vessels containing the samples into the instrument•
Put the prepared glass sample tubes into the rubber couplings•
Close unused positions with the supplied stoppers Ø 45 mm on the suction tube•
Put the caps onto the digestion vessels and install the sample aspiration tube•
As soon as the sample starts boiling switch on the waterjet pump.•
The hydrolysis time depends on the sample. From the start of the boiling on it takes a minimum of •
15 and a maximum of 60 minutes (usually 30 minutes is an appropriate time).
6 Operation
Foaming can be suppressed by the addition of small acid injections
(HCl 4 mol/l). It is recommended to switch on the external pump
only after the samples are boiling evenly and no further foam is to be
expected. Thus a premature sample extraction is avoided.
Fig. 6.7: Hydrolysis
6.7 Filtration
Fig. 6.8: Filtration
After expiration of the hydrolysis time, switch off the heating and
add hot distilled water (40 - 50 °C) to each digestion vessel to a
level of approx. 1 cm under the sample aspiration tube.
To filter the hydrolysate solution over the glass sample tubes, raise
the lift device to immerse the sample aspiration tubes into the solution. As soon as the contents of the individual digestion vessels are
completely transferred, rinse them with hot distilled water
(40 - 50 °C) portion by portion. The rinse volume until neutral
reaction is approx. 250 ml per sample.
19 B-411/E-416 Operation Manual, Version E
6.8 Drying the hydrolyzed samples
Before drying the hydrolyzed samples stir the sample layer and the celite layer (without touching the
sand layer) with a spatula to loosen the pulp. Afterwards wipe the spatula with fat free cotton wool or a
tissue and put the tissue into the sample.
Dry the glass sample tubes in a vacuum oven (≤ 4 h at 100°C/200mbar), in an oven (≤ 8 h at 100°C)
or in a microwave oven. Using a microwave oven makes the drying much faster. However, it is more
awkward due to the fact that the temperature in the sample can easily become too high (> 105 °C) if
an inappropriate heating power has been chosen.
Therefore, we suggest the following:
Step: 18 minutes with an output of 640 W1.
Step: 10 minutes with an output of 480 W for 4 samples 2.
13 minutes with an output of 480 W for 6 samples
The optimal parameters for the microwave may depend on the model.
The sample is dry when there is no moisture to be seen on the glass wall.
6 Operation
ATTENTION
The plastic holders for the glass sample tubes and paper thimbles for the E-812/E-816 are not
microwavable.
6.9 Preparing the extraction
Fill the dried, cooled, glass sample tubes with 20 g of quartz sand (grain size 0.3-0.9 mm). They are
now ready for extraction. The glass sample tubes are optimized for use either with the Extraction
System B-811 or with the Extraction Unit E-812/816.
6.10 Extraction
Buchi offers a wide range of fully automated ex-
traction units. Please consult the corresponding
brochures for detailed information.
Extraction Unit E-812 Soxhlet (2 positions)•
Extraction Unit E-812 Hot Extraction (2 posi-•
tions)
Extraction Unit E-816 Soxhlet (6 positions)•
Extraction Unit E-816 Hot Extraction (6 posi-•
tions)
Extraction System B-811 Standard (4 posi-•
tions)
Extraction System B-811 LSV (4 positions)•
Fig. 6.9: E-816 Hot Extraction
20 B-411/E-416 Operation Manual, Version E
7 Maintenance
This chapter gives instructions on all maintenance work to be performed in order to keep the instrument in good working condition.
WARNING
All maintenance and repair work requiring the opening or removal of instrument covers may only be
carried out by trained personnel and with the tools provided for this purpose.
WARNING
Electrical hazard:
Prior to all maintenance work on the instrument switch off the power supply and remove all •
sources of flammable vapor.
ATTENTION
Always wear personal protective equipment such as protective eye goggles, protective clothing and
gloves when maintaining the instrument.
7 Maintenance and repairs
7.1 Housing
Check the housing for defects (switches, plugs) and clean it regularly with a damp cloth and common
chromium steel cleaners.
ATTENTION
Never use organic solvents (except for ethanol) as cleaning agents as these might damage the instrument.
As the housing is made of stainless steel and plastic, any acid drops must be wiped away imme-•
diately with a damp cloth.
7.2 Heating chamber
ATTENTION
The surface of the heating chamber can get very hot. Always let the instrument cool down sufficiently
before carrying out any maintenance work at the heating chamber.
Remove the upper insulation plate a above the
heating chamber.
You can now clean the insulation with a moist
cloth or a vacuum cleaner.
1
Fig. 7.1: Cleaning the heating chamber
21 B-411/E-416 Operation Manual, Version E
7.3 Glass parts
The glass parts can be taken out and cleaned with commercially available cleaning agents or in an
ultrasonic bath. After the glass parts have been cleaned and fully dried, check each part visually for
cracks, scratches and for any parts or sections that might have splintered off. Take out and replace
any damaged glass parts.
7.4 Rubber couplings
Rinse the rubber couplings regularly with water.
7.5 Functional test
A functional inspection should be carried out annually or in case of weak performance of the instrument. To carry it out, proceed as follows:
Clean the instrument as described above in this chapter.•
Visually inspect the instrument, the lift device, the connecting cable, the glass parts and the hose •
connections.
Start-up the instrument with the energy regulator in the preheating position and check, whether a •
preheating is done.
In the case of technical problems, contact a Buchi customer service office.•
7 Maintenance and repairs
7.6 Main fuses
Fig. 7.2: Rear view of the instrument
Please change the main fuses as follows:
Pull out the fuse holder • a
Replace the defective mains fuse with a new •
one with same performance:
- 230V: T 5A L250V•
- 120V: T10A L250V•
Reinsert the fuse holder•
1
22 B-411/E-416 Operation Manual, Version E
7.7 Customer service
Only authorised service personnel is allowed to perform repair work on the instrument. These persons
have a comprehensive technical training and knowledge of possible dangers which might arise from
the instrument.
Addresses of official Buchi customer service offices are given on the Buchi website under:
www.buchi.com. If malfunctions occur on your instrument or you have technical questions or application problems, contact one of these offices.
The customer service offers the following:
Spare part delivery•
Repairs•
Technical advice•
7 Maintenance and repairs
23
B-411/E-416 Operation Manual, Version E
8 Shutdown, storage, transport and disposal
8 Shutdown, storage, transport and disposal
This chapter instructs how to shut down the instrument, how to pack it for storage or transport, and
specifies the storage and shipping conditions.
8.1 Storage and transport
WARNING
Unplug the mains cable and remove and thoroughly rinse the suction tube. Clean the instrument
thoroughly, remove all chemical residues and wash the glass parts.
Store and transport the instrument in its original packaging.
8.2 Disposal
To dispose of the instrument in an environmentally friendly manner, a list of materials is given in
chapter 3. This helps to ensure that the components are separated and recycled correctly. Make
especially sure to dispose of the gas springs appropriately.
Please follow valid regional and local laws concerning disposal.
24 B-411/E-416 Operation Manual, Version E
9 Spare parts
This chapter lists spare parts, accessories, and options including their ordering information.
Order the spare parts from Buchi. Always state the product designation and the part number when
ordering spare parts.
Use only genuine Buchi consumables and genuine spare parts for maintenance and repair to assure
good system performance and reliability. Any modifications to the spare parts used are only allowed
with the prior written permission of the manufacturer.
Table 10-1: Spare parts
Product Order number
Set of 4 digestion vessels, 300 ml 37377
Set of 4 glass sample tubes for B-811 37281
Set of 2 glass sample tubes for E-812/816 49430
Suction tube for B-411 37387
Suction tube for E-416 42868
Sample aspiration tubes 37380
Set of 4 stoppers, Ø 45 mm 37725
Set of 4 caps for digestion vessels 37463
Upper insulation plate for B-411 37416
Upper insulation plate for E-416 26736
Vacuum hose, Ø 6 x 16 mm 17622
Set rubber socket connection Viton, 4 pieces 44422
Rubber socket connection EPDM, 1 piece 37381
9 Spare parts
25
B-411/E-416 Operation Manual, Version E
10 Declarations and requirements
10.1 FCC requirements (for USA and Canada)
English:
This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to both Part 15 of the FCC Rules and the radio interference regulations of the Canadian
Department of Communications. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment.
This equipment generates, uses and can radiate radio frequency energy and, if not installed and used
in accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
Français:
Cet appareil a été testé et s'est avéré conforme aux limites prévues pour les appareils numériques
de classe A et à la partie 15 des réglementations FCC ainsi qu’à la réglementation des interférences
radio du Canadian Department of Communications. Ces limites sont destinées à fournir une protection adéquate contre les interférences néfastes lorsque l’appareil est utilisé dans un environnement
commercial.
Cet appareil génère, utilise et peut irradier une énergie à fréquence radioélectrique, il est en outre
susceptible d’engendrer des interférences avec les communications radio, s’il n’est pas installé et
utilisé conformément aux instructions du mode d’emploi. L’utilisation de cet appareil dans les zones
résidentielles peut causer des interférences néfastes, auquel cas l’exploitant sera amené à prendre les
dispositions utiles pour palier aux interférences à ses propres frais.
10 Declarations and requirements
26 B-411/E-416 Operation Manual, Version E
10.2 Declaration of conformity
We BÜCHI Labortechnik AG
do hereby declare on our responsibility that the products:
Hydrolysis Unit B-411/E-416
which is the object of this certification, is in accordance with the following norms:
EN 61010-1:2001 (~ IEC 61010-1) Safety requirements for electrical equipment for measurement,
control, and laboratory use
Part 1: General requirements
EN 55011:1998 + A1:1999 (IEC/CISPR 11:1997, modified + A1:1999) Industrial, scientific and
medical (ISM) radio-frequency equipment - Radio disturbance characteristics - Limits and methods of
measurement
10 Declarations and requirements
EN 61000-3-2:2000 Limits for harmonic current emissions
EN 61000-3-3:2000 Limitations of voltage fluctuations and flicker
In accordance with the regulations of the EU guidelines
73/23/EEC (electrical operating equipment/low-voltage guidelines)
89/336/EEC (electromagnetic compatibility)
Flawil, 12.07.07
BÜCHI Labortechnik AG
Meierseggstrasse 40
Postfach
CH-9230 Flawil 1
Switzerland
Tel.: +41 (0)71 394 63 63 Guido Worch
Fax: +41 (0)71 394 65 65 Quality Manager
buchi@buchi.com
www.buchi.com
27 B-411/E-416 Operation Manual, Version E
BÜCHI Labortechnik AG
CH-9230
T +41 71 394 63 63
F +41 71 394 65 65
Flawil 1 / Switzerland
www.buchi.com Quality in your hands
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