Bio-Rad Molecular Mass Rulers User Manual
EZ Load™Molecular Rulers
Catalog Numbers
170-8351 20 bp
170-8352 100 bp
170-8353 100 bp PCR
170-8354 500 bp
170-8355 1 kb
170-8356 Precision Mass
EZ Load Molecular Rulers
Quantity DNA sufficient for 100 lanes when used at 5 µl
per lane.
Storage Should be stored at 4 °C. Use only sterile pipet
tips when removing aliquots. Introduction of
nucleases will shorten shelf life.
Shipping Shipped at room temperature.
Shelf Life Stable for 1 year when stored at 4 °C.
5x Nucleic Acid Sample Loading Buffer
Quantity 1 ml
Storage Should be stored at room temperature. Use only
Shipping Shipped at room temperature.
Shelf Life Stable for 1 year when stored at room
Concentration 25% glycerol, 50 mM Tris pH 8.0, 5 mM EDTA,
Use Typically, 1 µl of 5x nucleic acid sample loading
sterile pipet tips when removing aliquots.
temperature.
0.2% bromophenol blue, 0.2% xylene cyanole
FF.
buffer is needed for every 4 µl of sample.
1
EZ Load 20 bp Molecular Ruler
Catalog Number 170-8351
Use Load 5 µl per lane. This loading translates into
500 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
20 bp PCR molecular ruler can be resolved in
1.5–2.5% standard agarose gels, Amplisize
agarose gels up to 4%, and polyacrylamide gels
up to 8%.
Contents 1 vial EZ Load 20 bp molecular ruler, 500 µl
supplied in 5% glycerol, 15 mM Tris pH 8.0,
1.5 mM EDTA, 0.04% bromophenol blue,
0.04% xylene cyanole FF.
Concentration 100 µg/ml
Size 50 bands: 20–1,000 bp in exact 20 bp
increments. A visually distinct reference band at
200 bp contains three times the concentration of
material found in the other bands.
Please note The EZ Load 20 bp molecular ruler does not
always resolve well in gels made of pure
NuSieve GTG. Use Bio-Rad Amplisize Agarose,
or the recommended mixture of 3:1 NuSieve
GTG: standard agarose.
1,000 bp
200 bp
20 bp
Fig. 1. 5 µl of the EZ Load 20 bp molecular ruler was loaded onto a 2.5%
Amplisize agarose gel.The gel was run at 140 V for 3 hours in 1x TBE
buffer. The gel was stained in EtBr for 15 minutes and destained in dH
30 minutes.
O for
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