Bio-Rad Molecular Mass Rulers User Manual

EZ Load™Molecular Rulers

Catalog Numbers

170-8351 20 bp
170-8352 100 bp
170-8353 100 bp PCR
170-8354 500 bp
170-8355 1 kb
170-8356 Precision Mass

EZ Load Molecular Rulers

Quantity DNA sufficient for 100 lanes when used at 5 µl

per lane.

Storage Should be stored at 4 °C. Use only sterile pipet

tips when removing aliquots. Introduction of
nucleases will shorten shelf life.

Shipping Shipped at room temperature.
Shelf Life Stable for 1 year when stored at 4 °C.

5x Nucleic Acid Sample Loading Buffer

Quantity 1 ml
Storage Should be stored at room temperature. Use only

Shipping Shipped at room temperature.
Shelf Life Stable for 1 year when stored at room

Concentration 25% glycerol, 50 mM Tris pH 8.0, 5 mM EDTA,

Use Typically, 1 µl of 5x nucleic acid sample loading

sterile pipet tips when removing aliquots.

temperature.

0.2% bromophenol blue, 0.2% xylene cyanole
FF.

buffer is needed for every 4 µl of sample.

1

EZ Load 20 bp Molecular Ruler

Catalog Number 170-8351

Use Load 5 µl per lane. This loading translates into

500 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
20 bp PCR molecular ruler can be resolved in

1.5–2.5% standard agarose gels, Amplisize
agarose gels up to 4%, and polyacrylamide gels
up to 8%.

Contents 1 vial EZ Load 20 bp molecular ruler, 500 µl

supplied in 5% glycerol, 15 mM Tris pH 8.0,

1.5 mM EDTA, 0.04% bromophenol blue,

0.04% xylene cyanole FF.

Concentration 100 µg/ml
Size 50 bands: 20–1,000 bp in exact 20 bp

increments. A visually distinct reference band at
200 bp contains three times the concentration of
material found in the other bands.

Please note The EZ Load 20 bp molecular ruler does not

always resolve well in gels made of pure
NuSieve GTG. Use Bio-Rad Amplisize Agarose,
or the recommended mixture of 3:1 NuSieve
GTG: standard agarose.

1,000 bp

200 bp

20 bp

Fig. 1. 5 µl of the EZ Load 20 bp molecular ruler was loaded onto a 2.5%
Amplisize agarose gel.The gel was run at 140 V for 3 hours in 1x TBE

buffer. The gel was stained in EtBr for 15 minutes and destained in dH
30 minutes.

O for

2

2

3

EZ Load 100 bp Molecular Ruler

Catalog Number 170-8352

Use Load 5 µl per lane. This loading translates into

250 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
100 bp molecular ruler can be resolved in
1–2.5% agarose gels and polyacrylamide gels up
to 8%.

Contents 1 vial EZ Load 100 bp molecular ruler, 500 µl

supplied in 5% glycerol, 15 mM Tris pH 8.0,

1.5 mM EDTA, 0.04% bromophenol blue,

0.04% xylene cyanole FF.

Concentration 50 µg/ml
Size 10 bands: 100–1,000 bp in exact 100 bp increments.
Please note The EZ Load 100 bp molecular ruler may show a

double- or triple-banding pattern in poly­acrylamide gels.

1,000 bp

100 bp

Fig. 2. 5 µl of the EZ Load 100 bp molecular ruler was loaded onto a

2.5% Amplisize agarose gel.The gel was run at 140 V for 3 hours in

1x TBE buffer. The gel was stained in EtBr for 15 minutes and destained in
dH

O for 30 minutes.

2

4

5

EZ Load 100 bp PCR Molecular Ruler

Catalog Number 170-8353

Use Load 5 µl per lane. This loading translates into

400 ng of DNA per lane. Adjustments may be
made to the loading volume for different well sizes
and desired band intensity. The EZ Load 100 bp
PCR molecular ruler can be resolved in 0.8–2%
agarose gels and polyacrylamide gels of 5%.

Contents 1 vial EZ Load 100 bp PCR molecular ruler,

500 µl supplied in 5% glycerol, 15 mM Tris
pH 8.0, 1.5 mM EDTA, 0.04% bromophenol
blue, 0.04% xylene cyanole FF.

Concentration 80 µg/ml
Size 30 bands: 100–3,000 bp in exact 100 bp

increments. Two visually distinct reference bands
at 1 kb and 3 kb contain three times the
concentration of material found in the other bands.

3,000 bp

1,000 bp

100 bp

Fig. 3. 5 µl of the EZ Load 100 bp PCR molecular ruler was loaded onto a

0.8% Molecular Biology Certified agarose gel.The gel was run at 140 V for

2.5 hours in 1x TBE buffer. The gel was stained in EtBr for 15 minutes and
destained in dH

6

O for 30 minutes.

2

7

EZ Load 500 bp Molecular Ruler

Catalog Number 170-8354

Use Load 5 µl per lane. This loading translates into

400 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
500 bp molecular ruler can be resolved in
agarose gels of up to 2%.

Contents 1 vial EZ Load 500 bp molecular ruler, 500 µl

supplied in 5% glycerol, 15 mM Tris pH 8.0,

1.5 mM EDTA, 0.04% bromophenol blue,

0.04% xylene cyanole FF.

Concentration 80 µg/ml
Size 16 bands: 500–8,000 bp in exact 500 bp

increments. A visually distinct reference band at
5 kb contains three times the concentration of
material found in the other bands.

5,000 bp

500 bp

Fig. 4. 5 µl of the EZ Load 500 bp molecular ruler was loaded onto a 0.8%
Molecular Biology Certified agarose gel.The gel was run at 140 V for

2.5 hours in 1x TBE buffer. The gel was stained in EtBr for 15 minutes and
destained in dH

8

O for 30 minutes.

2

9

EZ Load 1 kb Molecular Ruler

Catalog Number 170-8355

Use Load 5 µl per lane. This loading translates into

400 ng of DNA per lane. Adjustments may be
made to the loading volume for different well
sizes and desired band intensity. The EZ Load
1 kb molecular ruler can be resolved in agarose
gels of up to 2%.

Contents 1 vial EZ Load 1 kb molecular ruler, 500 µl

supplied in 5% glycerol, 15 mM Tris pH 8.0,

1.5 mM EDTA, 0.04% bromophenol blue,

0.04% xylene cyanole FF.

Concentration 80 µg/ml
Size 15 bands: 1–15 kb in exact 1 kb increments. A

visually distinct reference band at 5 kb contains
three times the concentration of material found
in the other bands.

15 Kb

5 Kb

1 Kb

Fig. 5. 5 µl of the EZ Load 1 kb molecular ruler was loaded onto a 0.7%
Molecular Biology Certified agarose gel.The gel was run at 140 V for

2.5 hours in 1x TBE buffer. The gel was stained in EtBr for 15 minutes and
destained in dH

O for 30 minutes.

2

1110

EZ Load Precision Molecular Mass
Standard

Catalog Number 170-8356

Use Load 5 µl per lane. Adjustments may be made to

the loading volume for different well sizes and
desired band intensity. The EZ Load Precision
Molecular Mass Standard can be resolved in
agarose gels of up to 2% and polyacrylamide
gels up to 8%.

When used at 5 µl per lane:
1,000 bp 100 ng
700 bp 70 ng
500 bp 50 ng
200 bp 20 ng

100 bp 10 ng

Contents 1 vial EZ Load Precision Molecular Mass

Standard, 500 µlsupplied in 5% glycerol, 15 mM
Tris pH 8.0, 1.5 mM EDTA, 0.04% bromophenol
blue, 0.04% xylene cyanole FF.

Concentration 50 µg/ml
Determination The concentration values of the EZ Load

of concentration Precision Molecular Mass Standard fragments

Please note To maintain accurate fragment concentrations, it

are determined by UV absorbance at 260 nm
wavelength using the conversion factor:

1.00 A
concentrations are accurate to within ± 1%.

unit = 50 µg/ml DNA. Fragment

260

is imperative to spin down any condensate and
mix contents thoroughly before opening tubes
and withdrawing aliquots.

12

1,000 bp

700 bp

500 bp

200 bp

100 bp

Fig. 6. 5 µl of the EZ Load Precision Molecular Mass Standard was
loaded onto a 1.8% Molecular Biology Certified agarose gel.The gel was

run at 70 V for 75 minutes in 1x TBE buffer. The gel was stained in EtBr for
15 minutes and destained in dH

O for 30 minutes.

2

13

Ordering Information

Catalog
Number Product Description

162-0144 AmpliSize Agarose, 50 g
162-0133 Molecular Biology Certified Agarose, 100 g
161-0767 5x Nucleic Acid Sample Loading Buffer, 10 ml

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Bio-Rad Laboratories, 2000 Alfred Nobel Drive, Hercules, CA 94547

4006137 Rev B