Bio-Rad Mini-PROTEAN II Multiscreen Apparatus User Manual
Mini-PR OTEAN®II
Multiscreen Apparatus
Instruction Manual
Catalog number
170-4017
For Technical Service Call Your Local Bio-Rad Office or in the U.S. Call 1-800-4BIORAD (1-800-424-6723)
Table of Contents
Page
Section 1 Introduction .............................................................................................1
1.1 Specifications ........................................................................................................1
Section 2 Equipment and Reagents........................................................................1
Section 3 Special Handling Features......................................................................3
3.1 Chemical Stability.................................................................................................3
Section 4 Multiscreen Assembly Procedure..........................................................3
4.1 Preparation for the Immunoassay in the Multiscreen Apparatus.........................3
4.2 Assembly of the Multiscreen Apparatus...............................................................3
4.3 Sample Loading and Washing ..............................................................................5
4.4 Color Development of Enzyme Conjugates.........................................................7
4.5 Detection with Colloidal Gold Conjugates...........................................................8
Section 5 Solution for Immunoassay Applications...............................................8
Section 6 References.................................................................................................9
Section 1
Introduction
With the Mini-PROTEAN II multiscreen apparatus you can quickly and easily screen up to
40 different antibody or serum samples on Western blots, without having to cut the membranes into
strips. Only 600 µl of sample is used per channel, eliminating waste of precious antibody. Two individual and independent sample templates allow screening of either one or two mini blots.
The multiscreen apparatus is simple to operate. Electrophorese an antigen sample on an SDSPAGE mini gel using the Mini-PROTEAN II cell, and blot it onto nitrocellulose or Zeta-Probe
®
membrane with the Mini Trans-Blot®, Trans-Blot, or Trans-Blot SD cell. After blocking the unreacted sites, clamp the membrane between the gasket and sample template. The assembly is held
together with four screws, and the rubber sealing gasket prevents any well-to-well leakage. Pipet
serum or antibody samples into each of the channels for incubation with the anitgen. Wash solutions can be easily introduced with the Eppendorf®Repeater™pipet, and are rapidly removed by
vacuum aspiration. The Multiscreen apparatus is compatible with all common Western blotting
procedures.
1.1 Specifications
Materials
Multiscreen apparatus Acrylic plastic
Multiscreen gasket Silicone rubber
Shipping weight 1.4 kg
Overall size 11 x 27 x 6 cm (W x L x H)
Membrane size 7 x 8.4 cm
Channel dimensions 2.5 mm x 5.2 cm x 5 mm (W x L x H)
Channels per sample template 20
Section 2
Equipment and Reagents
Catalog Number Product Description
170-4017 Mini-PROTEAN II Multiscreen Apparatus, includes Multiscreen
sample templates, 2 Gaskets and Base Plate
170-4018 Mini-PROTEAN II Multiscreen Gaskets, 2
Mini-Protean II Cell
165-2940 Mini-PROTEAN II Cell, includes 10 well combs, 0.75 mm
spacers (4), Electrode Core with Gaskets, lower buffer
chamber, lid with cables, 3 sets Glass Plates, Clamp
Assemblies (2), Casting Stand with Gaskets, leveling bubble,
and instructions
Transfer Cells
170-3930 Mini Trans-Blot Electrophoretic Transfer Cell
170-3935 Mini Trans-Blot Module
170-3946 Trans-Blot Electrophoretic Transfer Cell, with plate electrodes
170-3910 Trans-Blot Electrophoretic Transfer Cell, with standard electrodes
170-3940 Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
1
Catalog Number Product Description
Power Supplies
165-5052 PowerPac 200 Power Supply, 100/120 V
165-5053 PowerPac 200 Power Supply, 220/240 V
Related Instruments
170-6545 Bio-Dot
®
Microfiltration Apparatus
170-6542 Bio-Dot SF Microfiltration Apparatus
Blotting Media
Nitrocellulose Membrane (0.45 micron)
162-0115 Nitrocellulose Membrane, roll, 33 cm x 3 m, 1
162-0113 Nitrocellulose Membrane, sheets, 20 x 20 cm, 5
162-0116 Nitrocellulose Membrane, sheets, 15 x 15 cm, 10
162-0114 Nitrocellulose Membrane, sheets, 15 x 9.2 cm, 10
162-0117 Nitrocellulose Membrane, sheets, 9 x 12 cm, 10
162-0145 Nitrocellulose Membrane, sheets, 7 x 8.4 cm, 10
Nitrocellulose Membrane (0.2 micron)
162-0112 Nitrocellulose Membrane, roll, 33 cm x 3 cm, 1
162-0146 Nitrocellulose Membrane, sheets, 7 x 8.4 cm, 10
162-0147 Nitrocellulose Membrane, sheets, 13.5 x 16.5 cm, 10
Immun-Blot
®
Assay Kits
Bio-Rad's Immun-Blot assay kits contain the necessary components and instructions for
performing immune detection assays on blotted membranes.
170-6460 Immun-Blot Assay Kit - Goat Anti-Rabbit IgG (H+L)
AP Conjugate
170-6461 Immun-Blot Assay Kit - Goat Anti-Mouse IgG (H+L)
AP Conjugate
170-6462 Immun-Blot Assay Kit - Goat Anti-Human IgG (H+L)
AP Conjugate
170-6463 Immun-Blot Assay Kit - Goat Anti-Rabbit IgG (H+L)
HRP Conjugate
170-6464 Immun-Blot Assay Kit - Goat Anti-Mouse IgG (H+L)
HRP Conjugate
170-6465 Immun-Blot Assay Kit - Goat Anti-Human IgG (H+L)
HRP Conjugate
170-6466 Immun-Blot Assay Kit - Protein A HRP
170-6467 Immun-Blot Assay Kit - Protein G HRP
Total Protein Detection Kits
170-6512 Biotin-Blot Protein Detection Kit
170-6517 Enhanced Colloidal Gold Total Protein Detection Kit
Blotting Standards
161-0305 Prestained SDS-PAGE Standards, Low Range
161-0309 Prestained SDS-PAGE Standards, High Range
161-0318 Prestained SDS-PAGE Standards, Broad Range
161-0307 Biotinylated SDS-PAGE Standards Kit, Low Range, HRP
161-0308 Biotinylated SDS-PAGE Standards Kit, Low Range, AP
161-0312 Biotinylated SDS-PAGE Standards Kit, High Range, HRP
161-0313 Biotinylated SDS-PAGE Standards Kit, High Range, AP
161-0321 Biotinylated SDS-PAGE Standards Kit, Broad Range, HRP
161-0322 Biotinylated SDS-PAGE Standards Kit, Broad Range, AP
2
Loading...