Bio-Rad Foresight Chromatography Columns, Prepacked User Manual

Foresight™
RoboColumn Units
200 and 600 µl

Instruction Manual

Catalog Numbers

732-4801 732-4816
732-4802 732-4817
732-4804 732-4819
732-4805 732-4820
732-4807 732-4822
732-4808 732-4823
732-4813 732-4825
732-4814 732-4826

Bio-Rad Technical Support

For help and technical advice, please contact the Bio-Rad
Technical Support department. In the United States, the Technical
Support department is open Monday–Friday, 5:00 AM to 5:00 PM,
Pacific Time.

Phone: [phone]

Fax: [phone]

Email: [e-mail] (for U.S. and
international customers)

Online technical support and worldwide contact information are
available at www.consult.bio-rad.com.

Legal Notices

RoboColumn is a trademark of Atoll GmbH.

Freedom EVO, Freedom EVOware, Te-Chrom, and Te-Stack are
trademarks of Tecan Group Ltd.

© 2012 by Bio-Rad Laboratories, Inc. All rights reserved.

Table of Contents

Bio-Rad Technical Support ii
Legal Notices ii

Section 1 Introduction 1

Section 2 Characteristics 2

Section 3 Advice on Handling 4

3.1 Equipment 4

3.2 Sample Preparation 6

3.3 Experiment Setup 6

3.4 Te-Chrom Wizard 7

Section 4 Protocol 8

4.1 Detailed Protocol 8

Section 5 Troubleshooting Guide 15

Section 6 Ordering Information 16

Section 1
Introduction

Foresight™ RoboColumn units are prepacked chromatography
microcolumns used for rapid process development. Their design
is fully compatible with robotic liquid handling workstations, such
as the Freedom EVO series from Tecan, and allows process
developers to perform high throughput (HT) parallel screening
and experimentation. Foresight RoboColumn units may be used
for qualitative media screening experiments and/or small scale
methods development. Through design of experiments (DoE)
utilizing Foresight RoboColumn units, an operational window can
be defined to aid in developing a quality by design (QbD) process.

The design of the Foresight column offers:

1. Convenience

The prepacked columns used in conjunction with a robotic
liquid handling device allow users to create automated
protocols for HT experimentation. The ability to define a design
space enables process scientists to develop robust purification
processes.

2. Reliability

Each column is individually packed under optimum
compression, ensuring consistent experimental results.

3. Efficiency

The small column design enables users to use precious
feed sample more efficiently while performing real
chromatographic separations in parallel to determine critical
quality parameters.

Foresight RoboColumn units are available in a variety of
chromatography modes including anion, cation, and mixed-mode.
See Section 6, Ordering Information, for the complete product line.

Technical Support: 1-800-424-6723 • www.bio-rad.com 1

Section 2
Characteristics

Foresight™ RoboColumn units are ready-to-use chromatography
microcolumns prepacked with Bio-Rad Laboratories process
chromatography resins. Each row contains eight columns
and either a single row or multiple rows may be used. For HT
screening, configuration into 96-well plate format is possible with
the use of an array plate.

Foresight RoboColumn units are available in two volumes:
200 and 600 µl.

Table 1. Characteristics of Foresight RoboColumn units.

Plate size 128.3 x 86.0 x 14.0 mm

Plate material Polyoxymethylene copolymer

Number of wells Polypropylene/polyethylene

Column inner diameter 5 mm

Column material Polypropylene

Chemical stability Stable in organic solvents. UNOsphere™ and

Avoid Halogenated organic solvents, hexane

Storage solution • UNOsphere Q, UNOsphere S, UNOsphere

Recommended
storage temperature

Working temperature 4–30°C

Maximum working
pressure

Flow velocity 16–1000 cm/h

Laser label Placed on every individual column body and

*

Specifications for all Bio-Rad chromatography media may be found by
visiting www.bio-rad.com/process and downloading their corresponding
instruction manual.

Nuvia™ resins are stable in commonly used
aqueous buffers pH 1–14. CHT™ resin is stable in
aqueous buffers of pH >60.5.*

Rapid S, Nuvia™ cPrime™: 20% ethanol

• CHT Type I, 40 µm and CHT Type II, 40 µm:
20% ethanol + 0.01M Na phosphate, pH 6.8

• Nuvia Q: 20% ethanol + 1M NaCl

• Nuvia S: 20 % ethanol + 0.1M NaCl

4–30°C

Up to 8.0 bar

2

containing:

• Product number

• Resin name

• Column volume in µl

2 Technical Support: 1-800-424-6723 • www.bio-rad.com

Table 2. List of Bio-Rad chromatography media.

Chromatography
Media

UNOsphere Q Strong Anion 4110109

UNOsphere S Strong Cation 4110109

UNOsphere
Rapid S

Nuvia Q Strong Anion 10018215

Nuvia S Strong Cation 10018215

Nuvia cPrime

CHT Type I, 40 µm

CHT Type II, 40 µm

Mode

Strong Cation 10010339

Mixed Mode
(Hydrophobic-Cationic)

Mixed Mode
(Metal Affinity-Cationic)

Mixed Mode
(Metal Affinity-Cationic)

Instruction Manual
Literature #

10023853

6086

6086

Technical Support: 1-800-424-6723 • www.bio-rad.com 3

Section 3
Advice on Handling

3.1 Equipment

Foresight™ RoboColumn units are designed for robotic handling.
Table 3 is a guide to the equipment required for robotic handling of
the units using Tecan’s Freedom EVO workstation.

Table 3. Recommended equipment for automated handling of
Foresight RoboColumn units.

Equipment Details Tips and tricks

RoboColumn units
96-well array plate

Te-Chrom
Module (A)

Arrange up to
96 individual
columns onto
the 96-well array
plate according
to application
requirements

Use the Te-Chrom
module to integrate
the Foresight
RoboColumn units
onto the robotic
worktable

• Height
adjustable

• Waste option
(B)

• Do not mix short
(200 µl) and long
(600 µl) columns

• Make sure the
columns are securely
seated into the
96-well array plate
before using them

• Use the Te-Chrom
module in
combination
with a Te-Stack
plate stacker for the
optimized handling
and storage of
collection plates

• Use the waste
container to remove
buffers from each
step

• Make sure that the
columns are an
appropriate distance
from the collection
plate before starting
the experiment

4 Technical Support: 1-800-424-6723 • www.bio-rad.com