Bio-Rad Bio-Scale Mini Bio-Gel P-6 Desalting Cartridges User Manual
Bio-Scale™Mini
Bio-Gel®P-6 Desalting
Cartridges, 10 and 50 ml
Instruction Manual
Catalog #
732-5304, 732-5312, 732-5314
Table of Contents
Section 1...Introduction ........................................1
Section 2 Connecting to Bio-Rad’s
Low-Pressure Chromatography
Instruments ......................................24
Section 3 Connecting to Other Liquid
Chromatography Systems..................9
3.1 Connecting to BioLogic
DuoFlow
™
Systems .................9
3.2 HPLC Systems ......................10
3.3 FPLC Systems .......................11
Section 4 Preparing a Cartridge for Use...........12
4.1 Sample Preparation ...............13
Section 5...Desalting and Buffer Exchange.........14
5.1 With a Liquid Chromatography
System ..................................15
Section 6 Technical Assistance ........................16
Section 7 Ordering Information.........................17
Section 8 References .......................................19
Section 1
Introduction
Bio-Scale Mini cartridges have a patent-pending
double-wall design that provides extra durability and
allows easy, reliable runs with aqueous buffers
commonly used for protein purification. The
polypropylene luer fittings and internal sealing
surfaces assure leak-free operation at pressures up
to 45 psi. Bio-Scale Mini cartridges are convenient,
disposable, and supplied ready for use. They are
easy-to-use and prepacked for fast, reproducible
chromatographic separations. Cartridges are
available for a variety of chromatographic techniques,
including desalting, ion exchange and affinity
chromatography. The patent-pending design of
Bio-Scale Mini cartridges offers:
• Supplied ready for use; simply equilibrate the
cartridge in the buffer of choice
1
• Luer fittings for convenient connection to any
chromatography system
Bio-Scale Mini Bio-Gel P-6 cartridges are packed
with Bio-Gel
®
P-6 gel. This gel consists of
hydrophilic spherical polyacrylamide beads
designed for the purification of proteins, nucleic
acids, viruses, plasmids, and other
macromolecules. The Bio-Gel P-6 beads are
designed to provide high-efficiency desalting or
buffer exchange at low backpressure. Detailed
product information is given in Table 1. See
Ordering Information for a listing of the complete
Bio-Scale Mini cartridge product line.
2
Table 1. Bio-Scale Mini Bio-Gel P-6
cartridge specifications.
Size 10 and 50 ml bed volume
Dimensions 10 ml: 80 mm length x 12.6 mm inner
diameter
50 ml: 80 mm length x 28.2 mm inner
diameter
Maximum pressure tolerance 10 ml: 45 psi
50 ml: 40 psi
Recommended flow rates:
at ambient temperature
(18–24°C) 10 ml: 0.5–4.0 ml/min (24–192 cm/hr)
50 ml: 2.5–20.0 ml/min (24–192 cm/hr)
at cold room temperature
(4–8°C) 10 ml: 0.5–2.0 ml/min (24–96 cm/hr)
50 ml: 2.5–10.0 ml/min (24–96 cm/hr)
Fittings Female luer-lock fitting inlet and
male luer fitting oulet
Column material Polypropylene
Frit material Polyethylene (HDPE)
Shipping conditions 20 mM Bis-Tris, pH 6.5 + 0.05% azide
Storage recommendation 20 mM Bis-Tris, pH 6.5 + 0.05% azide
Autoclavability Not autoclavable
3
Section 2
Connecting to Bio-Rad’s
Low-Pressure
Chromatography Instruments
Bio-Scale Mini cartridges are ideal for use with
Bio-Rad's BioLogic
™
LP chromatography system,
Econo
™
gradient pump, the patented* Model EP-1
Econo
™
pump, and all low-pressure chromatography
instruments. Bio-Scale Mini cartridges can be conveniently connected directly to the system using the
luer fittings on the cartridge.
1. Install 1.6 mm inner diameter (ID) tubing in the
pumphead. Adjust platen pressure screw (on
pumphead) – using a screwdriver or coin, turn
the screw counterclockwise as far as it will go,
then turn clockwise three full turns. Assemble
with fittings and lock rings as shown in Figure 1.
*US Patent 5,135,658
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