Bio-Rad Bio-Plex Data Pro Software User Manual

MULTIPLEX SUSPENSION ARRAYS

Bio-Plex Data Pro™ Software

Getting Started

BIO-RAD TECHNICAL SUPPORT DEPARTMENT

The Bio-Rad Technical Support Department in the U.S. is open Monday through Friday, 5:00 a.m. to 5:00
p.m., Pacific Standard Time. Worldwide technical support is available on the Web at www.consult.bio-

rad.com.

Phone: [phone], option 2

Fax: [phone]

Email: [e-mail] (U.S.)

[e-mail] (International)

Web: www.consult.bio-rad.com

NOTICE

No part of this publication may be reproduced or transmitted in any form or by any means, electronic or
mechanical, including photocopy, recording, or any information storage or retrieval system, without
permission in writing from Bio-Rad.

Bio-Rad reserves the right to modify its products and services at any time. This user guide is subject to
change without notice. Although prepared to ensure accuracy, Bio-Rad assumes no liability for errors or
omissions, or for any damage resulting from the application or use of this information.

The following are trademarks of Bio-Rad Laboratories: Bio-Rad, Bio-Plex, Bio-Plex Manager, and 
Bio-Plex Data Pro. Luminex, xMAP, and xPONENT are trademarks of Luminex Corporation. Windows and
Excel are trademarks of Microsoft Corporation. Pentium is a trademark of Intel Corporation.

No rights or licenses under any of Luminex Corporation’s patents are granted by or shall be implied from
the sale or acquisition of this Bio-Plex system containing Luminex technology (the “System”) to you, the
end-user. By using this System, you agree that (i) the System is sold only for use with fluorescently
labeled microsphere beads authorized by Luminex (“Beads”), and (ii) you obtain rights under Luminex’s
patents to use this System by registering this System with Bio-Rad in accordance with the instructions
accompanying this System and purchasing a kit containing Beads.

Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Drive, Hercules, CA 94547

Copyright © 2001–2011 by Bio-Rad Laboratories. All rights reserved.

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Table of Contents

Welcome . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1 Installing Bio-Plex Data Pro™ 1.0 Software . . . . . . . . . . . . . . . . . . . . . . . . . . 5

2 Changing Your Screen Resolution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

3 Bio-Plex Data Pro™ Software Licensing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

4 An Overview of Bio-Plex Data Pro™ Software . . . . . . . . . . . . . . . . . . . . . . . 12

5 Analyzing Your Data . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21

6 Deactivating Bio-Plex Data Pro™ Software Licenses. . . . . . . . . . . . . . . . . . 25

7 Installing Bio-Plex Manager™ 6.1 Software . . . . . . . . . . . . . . . . . . . . . . . . . 27

8 Installing the NetHASP Network License Manager . . . . . . . . . . . . . . . . . . . . 29

9 Uninstalling Bio-Rad Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33

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Bio-Plex Data Pro Software Getting Started Guide

Welcome

Thank you for purchasing Bio-Rad Bio-Plex® software.

Bio-Plex Data Pro™ software meets your multiplex data analysis needs. Bio-Plex Data Pro software is a
data management and data visualization tool designed to allow you to quickly draw conclusions about
your biological study. It allows researchers to get answers to the complex questions that are at the heart
of their research. You can easily manage data from many instrument runs, quickly making comparisons
between experimental groups and drawing conclusions.

Bio-Plex Data Pro software is used together with Bio-Plex Manager™ software to provide a seamless
and integrated solution.

The major functions of Bio-Plex Manager software are:

• Evaluation of raw instrument data

• Optimization of standard curves and usable assay range

• Identification of outliers

• Identification and resolution of problems with instrument runs

Packaging Checklist

The software product you purchased will be in one of the following two configurations.

BIO-PLEX DATA PRO (1 CD)

If there is one CD in the folder, then the folder should include the following:

• Bio-Plex Data Pro Software Getting Started Guide (this document)

• Bio-Plex Data Pro Software Installation CD. The activation code is located on the folder
pocket. You will need this activation code to activate the Bio-Plex Data Pro license.

BIO-PLEX MANAGER (2 CDS)

If there are two CDs in the folder, then the folder should include the following:

• Bio-Plex Data Pro Software Getting Started Guide (this document).

• Bio-Plex Data Pro Software Installation CD. The activation code is located on the folder
pocket. You will need this activation code to activate the Bio-Plex Data Pro license.

• Bio-Plex Manager Software Installation CD.

• Release Notes — Bio-Plex Manager Software, Version 6.1

• A white envelope containing the Bio-Plex Manager USB Network Security HASP key. This
HASP key is used to manage the Bio-Plex Manager user licenses. You will need this HASP key
in section 8, Installing the NetHASP Network License Manager, on page 29.

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1 Installing Bio-Plex Data Pro™ 1.0 Software

Install Bio-Plex Data Pro™ software as described in the following sections.

Bio-Plex Data Pro System Requirements

The following are the minimum and recommended system requirements for Bio-Rad Data Pro.

Component Minimum Recommended

Operating system Windows XP Service Pack 3 Windows 7 (32-bit or 64-bit)
Processor Core 2, 2.6 GHz or higher Core 4, 3.1 GHz or higher
Hard disk space 160 GB 500 GB
System memory 2 GB 8 GB

Videographic memory
Screen resolution 1280 x 1024 1920 x 1080
Screen colors 24-bit True Color 24-bit True Color
Other software Internet Explorer 8.0

a. Bio-Rad recommends that you keep your video drivers up to date.

Installing the Bio-Plex Data Pro Software

To install the software, insert the Bio-Plex Data Pro CD into the CD-ROM drive on your computer and
follow the prompts. The installation should start automatically. If it does not, open a Windows Explorer
window and click the CD drive icon. Then double-click setup.exe to install the software.

a

Not applicable 1 GB

Microsoft Excel 2007

Internet Explorer 8.0
Microsoft Excel 2007

Note: You have the option of choosing a Typical or Custom installation. The only difference
between the installation types is that Custom allows you to specify where the software is
installed. The Typical option installs the product in Program Files/Bio-Rad/Bio-Rad Data Pro.

When the installation is completed, the Bio-Plex Data Pro icon appears on your desktop.

Luminex Output CSV Files

Customers using xPONENT software (all versions) to run their Luminex system must convert their files
before these files can be imported into Bio-Plex Data Pro. You must use Bio-Plex Manager 6.1 to convert
the Luminex Output CSV files. Conversion of data files of other third-party software is not supported.

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Bio-Plex Data Pro Software Getting Started Guide

2 Changing Your Screen Resolution

Your computer screen resolution must be set to the minimum settings to correctly display the Bio-Plex
Manager™ and Bio-Plex Data Pro™ interfaces. The status bar and some dialog boxes will not display
properly at lower resolutions. If your display is currently set to a lower resolution, then follow the
procedure for your operating system.

See Bio-Plex Manager System Requirements on page 27 and Bio-Plex Data Pro System Requirements
on page 5 for the screen resolution settings.

WINDOWS XP

To change the screen resolution on Windows XP:

1. Go to the Windows Start menu, select Settings, and select Control Panel.

2. Open the Display control panel.

3. In the Display Properties dialog, select the Settings tab.

4. Drag the Screen Area slider to the right (toward More) until you have selected the desired setting.
Click OK to accept the settings.

Figure 1. Screen Resolution Dialog Box (Windows XP)

WINDOWS 7

To change the screen resolution on Windows 7:

1. Right-click in your desktop, then click Personalize.

2. Click Display.

3. Click Adjust Resolution.

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4. Click the Resolution drop-down list and move the slider until it displays the desired setting.

Figure 2. Screen Resolution Dialog Box (Windows 7)

5. Click OK.

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Bio-Plex Data Pro Software Getting Started Guide

3 Bio-Plex Data Pro™ Software Licensing

This section describes the terms of the Bio-Plex Data Pro™ evaluation period, the Bio-Plex Data Pro
license, and how to activate your license online or offline.

Evaluation Period

There is a 30-day evaluation period with Bio-Plex Data Pro. During the evaluation period, you have full
access to all Bio-Plex Data Pro Plus product features. You may continue to use the product once the
evaluation period ends on any projects already created. If the evaluation period ends and you have not
activated your license, you will not be able to create new projects nor will you be able to import additional
data into existing projects. All other features will continue to be available, and you can continue to use
the sample projects.

Bio-Plex Data Pro Software License

Each Bio-Plex Data Pro license is a five-user (5) license; you can have up to five computers with active
licenses at any time. You can move a license from one computer to another by deactivating it on the first
and activating it on the second computer.

Activating Your License Online

You can activate your license at any time during the evaluation period.

Note: You must have Internet access in order to activate your license online.

To activate your license online:

1. Start Bio-Plex Data Pro.

2. In the Evaluate Bio-Plex Data Pro window, click Activate Bio-Plex Data Pro, then click Continue.

3. In the Activate Bio-Plex Data Pro window, enter the 18-digit activation code and click Activate.

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The 18-digit activation code can be found in the Bio-Plex Data Pro software product folder pocket.

Once the license is activated, the following window is displayed.

4. Click Exit.

Note: You can activate your license while the application is being run in evaluation mode.
Start Bio-Plex Data Pro in evaluation mode. To activate your license, select Manage License
from the Help menu and follow the prompts.

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Bio-Plex Data Pro Software Getting Started Guide

Activating Your License Offline

If you do not have Internet access or you want to activate your license offline, contact Bio-Rad Support
at 1-800-424-6723 or [e-mail] (email) to request an activation certificate.

Once you receive the activation certificate, follow the procedure to activate your Bio-Plex Data Pro
license.

To active your license offline:

1. Start Bio-Plex Data Pro.

2. In the Evaluate Bio-Plex Data Pro window, click Activate Bio-Plex Data Pro and click Continue.

3. In the Activate Bio-Plex Data Pro window, click the Activate License via Technical Support link.

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4. Create a text file and copy and paste the Installation ID number from the Import Activation
Certificate window into the file. Include the 18-digit activation code, which is found in the Bio-Plex
Data Pro software product folder in the text file. Attach the text file in an email to Technical Support
with the subject line: Request to Activate Bio-Plex Data Pro License.

Technical Support will process your email request and reply with a certificate and instructions for
completing the activation process.

5. When you receive the certificate, return to the Import Activation Certificate window. Copy and
paste the certificate information you receive from Technical Support into the text box.

6. Click Import.
The following window is displayed.

7. Click Exit.

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