Zeiss Stemi 305 Operating Manual

Operating Manual

Stereo microscope Stemi 305

You have acquired a product made by ZEISS. Please read this
operating manual before using your instrument for the first time to
make sure that its quality is maintained and you can utilize your
instrument reliably for a long time.

COPYRIGHT Stereo microscope
ZEISS Stemi 305

Knowledge of this manual is required for operation of the instrument. Would you therefore please
familiarize yourself with the contents of this manual and pay special attention to instructions concerning
the safe operation of the device.

We reserve the right to perform modifications in view of technical progress; this operating manual is not
subject to revision.

© Unless expressly authorized, forwarding and reproduction of this document as well as utilization

and communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.

All rights reserved in the event of granting of patents or registration of a utility model.

Issued by: Carl Zeiss Microscopy GmbH

Carl-Zeiss-Promenade 10
D-07745 Jena, Germany

[e-mail]
www.zeiss.com/microscopy

Carl Zeiss Microscopy GmbH
Königsallee 9-21
D-37081 Göttingen, Germany

SAP no.: 435063-7044-001

Date of issue (Version 6): 01/28/2015

4 435063-7044-001 01/2015

Stereo microscope CONTENTS
Stemi 305 ZEISS

CONTENTS

Page

1 INTRODUCTION ........................................................................................................... 7

1.1 General information ....................................................................................................... 7

1.2 Notes on instrument safety ............................................................................................ 8

1.3 Warranty notes ............................................................................................................ 12

2 DESCRIPTION ............................................................................................................. 13

2.1 Indication for use ......................................................................................................... 13

2.2 Microscope system ....................................................................................................... 13

2.3 Microscope sets and fields of application ...................................................................... 14

2.4 Interfaces on the microscope body Stemi 305 ............................................................... 15

2.5 System overview .......................................................................................................... 16

2.6 Technical data.............................................................................................................. 18

3 INSTALLATION ........................................................................................................... 21

3.1 General information ..................................................................................................... 21

3.2 Installing the stereo microscope .................................................................................... 21

3.3 Mounting optional components ................................................................................... 23

3.3.1 Mounting additional reflected-light illuminator ............................................................. 23

3.4 Connecting stereo microscope to the power supply ...................................................... 26

3.5 Switching stereo microscope ON or OFF ....................................................................... 26

4 OPERATION ............................................................................................................... 27

4.1 Adjusting the stereo microscope ................................................................................... 27

4.2 Setting reflected-light illumination ................................................................................ 28

4.2.1 Integrated vertical illuminator ....................................................................................... 28

4.2.2 Spot illuminator ........................................................................................................... 29

4.2.3 Double spot illuminator ................................................................................................ 29

4.2.4 Ring illuminator ........................................................................................................... 30

4.2.5 Controller K LED .......................................................................................................... 31

4.3 Transmitted-light illuminators ....................................................................................... 32

4.3.1 Transmitted-light module in stand K EDU...................................................................... 32

4.3.2 Transmitted-light unit in stand K LAB ............................................................................ 33

5 CARE, MAINTENANCE AND SERVICE ........................................................................ 34

5.1 Care ............................................................................................................................ 34

5.2 Maintenance ............................................................................................................... 35

5.2.1 Replacing the power unit ............................................................................................. 35

5.2.2 Opening the stand cover plate, stand K EDU ................................................................. 36

5.2.3 Opening the stand cover plate, stand K LAB ................................................................. 37

5.2.4

Wearing parts .............................................................................................................. 38

5.3 Service ......................................................................................................................... 38

5.4 Product disposal ........................................................................................................... 39

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CONTENTS Stereo microscope
ZEISS Stemi 305

6 APPENDIX ................................................................................................................... 40

6.1 List of abbreviations ...................................................................................................... 40

6.2 Troubleshooting ........................................................................................................... 41

6.3 List of illustrations ......................................................................................................... 42

6.4 Index ............................................................................................................................ 43

6 435063-7044-001 01/2015

Stereo microscope INTRODUCTION
Stemi 305 General information ZEISS

1 INTRODUCTION

1.1 General information

The stereo microscope Stemi 305 has been designed, produced and tested in compliance with the
standards DIN EN 61010-1 (IEC 61010-1) and IEC 61010-2-101 "Safety Requirements for Electrical
Measuring, Control and Laboratory Equipment".

The device meets the requirements of the EC Directive 98/79/EC Annex 1 regarding ivd products and the
EC RoHS Directive 2011/65/EC and carries the mark.

This operating manual contains information and warnings to be observed by the operator.

The devices must be disposed of in compliance with the WEEE Directive 2012/19/EC.

In this operating manual, the following warning and information symbols are used:

CAUTION
This symbol indicates a possible hazard to the user.

CAUTION
LED risk group 2 acc. to DIN EN 62471:2009 Optical radiation is emitted.
Do not look into the beam. This may damage your eyes.

CAUTION: High-energy UV radiation!

Risk of damage to eyes and skin!

CAUTION

Hot surface!

CAUTION

Remove the power plug before any intervention in the instrument!

ATTENTION

This symbol indicates a possible hazard to the instrument or system.

NOTICE
This symbol indicates information which deserves special attention.

01/2015 435063-7044-001 7

are no longer effective, the device must be

taken out of service and secured against being used again unintentionally. Please contact a

000 as well as the

Do not operate the devices included in the scope of supplies in a potentially explosive
atmosphere, in the presence of volatile anaesthetics or flammable solvents such as alcohol,

structed persons who have been informed about the

cation in

equipped with special equipment protecting them from corrosive,

light illuminators have been

energy light), never look

INTRODUCTION Stereo microscope
ZEISS Notes on instrument safety Stemi 305

1.2 Notes on instrument safety

If it becomes apparent that the safety measures

ZEISS service agency or the Carl Zeiss Microscopy Service to have the instrument repaired.

The Stemi 305 Information regarding instrument safety and installation conditions 435063­6044-008 and the Stemi 305 short operating instructions 435063-8044­operating instructions regarding the light sources also must be observed.

gasoline or similar substances.

The devices may only be operated by in
possible risks in conjunction with the use of microscopes and the fields of appli
question. The microscope is a high-precision device that may be impaired in its performance or
even destroyed if handled improperly.

Before commissioning the instrument, make sure that the existing power supply is suitable for
the instrument.

Make sure that the mains plug is removed from the socket before the instrument is opened.

The devices are not
potentially infectious, toxic and radioactive or other samples that may be hazardous to health. If
you handle such samples, be sure to observe all legal requirements, in particular the relevant
national accident prevention regulations.

The LED reflected-light illuminators and the LED transmitted­classified as belonging to LED risk group 2 acc. to DIN EN 62471:2009. Avoid looking directly
into the LED light.

If the device is operated with an external cold light source (high­directly into the fibre optic output of the cold light source. Otherwise, there is a risk of dazzling
and blindness.

Never cover the open light guide socket or the fibre optic output. There is a fire hazard!

In all cases avoid covering the open light guide socket or the fibre optic output with your hand
or other parts of your body. There is a risk of burns!

8 435063-7044-001 01/2015

Do not replace detachable mains cables by inadequately designed mains cables. Only the

The microscope and the controller K LED are equipped with a desktop power unit each

The desktop power unit meets the requirements of protection class II (with protective

Changing the lamps on the cold light source is only admissible in accordance with the
manufacturer's operating manual. If the appropriate instructions are not complied with, there is

hold waste; dispose of them in

Samples must also be disposed of appropriately in accordance with the applicable statutory

305 incl. its original accessories may only be used for the

ating manual. The manufacturer cannot assume any liability

or single components.
Changes to and maintenance on this device and on devices which are operated together with
the microscope may only be performed by our service or by authorized persons. The

r damage due to unauthorized

Dirt and dust may impair the performance of the device. The device must therefore be

Considerable temperature fluctuations, direct exposure to sunlight and vibrations should be

lation louvers free and do not

Stereo microscope INTRODUCTION
Stemi 305 Notes on instrument safety ZEISS

Safe disconnection from the power supply is ensured exclusively by removing the mains plug.
The switch on the microscope only switches into standby mode.

specified power supply lines may be used.

allowing line voltages in the range from 100 to 240 V ±10 %, 50 / 60 Hz, without the need for
changing the voltage setting on the device.

insulation). If its casing is damaged, put the power unit out of operation. The microscope may
only be operated with the desktop power unit supplied with the instrument.

a risk of burns and explosion when changing the lamp.

Defective devices should not be disposed of with house
compliance with the statutory provisions.

provisions and internal work instructions.

The stereo microscope Stemi
applications described in this oper
for other applications, including those of individual modules

manufacturer of the devices does not accept any liability fo
interventions in the device. Moreover, such interventions invalidate all guarantee / warranty
claims.

protected from such influences to the greatest possible extent and covered with the dust cover
when not in use. Before covering the device, always make sure that it is definitely switched OFF.

avoided.

Blocking or covering ventilation louvers may result in heat accumulation which may damage the
device and in extreme cases even cause fire. Always keep venti
insert or allow any objects to fall into them. All electrical components and parts must be located
at a distance of min. 15 cm from flammable objects and walls.

01/2015 435063-7044-001 9

refer to the appropriate operating instructions incl. the safety provisions contained in them

For transport over an extended distance, the device must be dismantled partially and

305. Only the connection cables supplied

305 is

INTRODUCTION Stereo microscope
ZEISS Notes on instrument safety Stemi 305

When operating a Stemi 305 cam, please refer to the appropriate operating instructions incl.
the safety provisions contained in them before commissioning the device.

If it is intended to operate the Stemi 305 with an external fibre optical cold light source, please

before commissioning the light source.

The desktop power unit must not get into contact with moisture.

transported in its original packaging.

To transport the device over an extended distance, use the original packaging or the transport
case Stemi 305/508.

To connect the LED illuminators of the Stemi 305 system, six-pin modular connectors are used
which are known from telecommunications (type: RJ 12).

Only the LED illuminators pertaining to the Stemi 305 may be connected to the RJ 12 sockets in
the microscope body and in the stands of the Stemi
with the device may be used.

The use of commercially available cables with RJ 12 connectors or the connection of fax units,
modems or other telecommunication equipment to the RJ 12 sockets of the Stemi
prohibited.

10 435063-7044-001 01/2015

Stereo microscope INTRODUCTION
Stemi 305 Warranty notes ZEISS

Fig. 1 Warning and information labels on the device

1 LED aperture, transmitted-light illuminators

2 LED aperture, spot illuminator K LED
3 LED aperture, ring illuminator K LED, segmentable
4 LED aperture, double spot illuminator K LED
5 LED aperture, integrated vertical illuminator

Fig. 2 Warning labels and apertures for LED radiation

01/2015 435063-7044-001 11

INTRODUCTION Stereo microscope
ZEISS Warranty notes Stemi 305

1.3 Warranty notes

The manufacturer guarantees that the device is free from material or manufacturing defects when
delivered. Any defects which may have occurred must be reported to us immediately and steps be taken
to minimize damage. If notified of such a defect, the device manufacturer is obligated to rectify it at his
discretion, either by repairing the instrument or by delivering an intact replacement. No guarantee is
provided for defects caused by natural wear (wearing parts in particular) and improper use.

The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or
any other tampering with the device, particularly the removal or replacement of instrument components,
or the use of accessories from other manufacturers. Such actions will render all warranty claims invalid.

No maintenance or repair work may be performed on the microscopes which exceed the activities
specified in this operating manual. Repair may only be performed by ZEISS Service or by persons
specifically authorized by it. Should any malfunctions occur on the device, please first contact the
Carl Zeiss Microscopy Service or, abroad, the ZEISS representative in your area.

12 435063-7044-001 01/2015

existing!

Stereo microscope DESCRIPTION
Stemi 305 Indication for use ZEISS

2 DESCRIPTION

2.1 Indication for use

The stereo microscopes Stemi 305 are microscopes for magnifying spatial observation of small objects.
They have been designed and built for training purposes in schools, universities and natural science
facilities. They are used, moreover, in biological and medical laboratories, in industrial manufacture and
quality assurance.

Stemi 305 is envisaged for applications in biology and in medicine for the analysis of blood and/or tissue
samples from the human body. Applications in the field of diagnostic medicine are explicitly excluded,
except for the field of medical research.

2.2 Microscope system

1 Eyepiece in eyepiece tube (eyepiece 10x/23)

2 Microscope body (trinocular) with installed

vertical illuminator

3 Interface for cameras with C-mount connector
4 Zoom button to adjust the magnification
5 Handle
6 Stand (model K EDU)
7 Focusing drive to focus the specimen

8 Rotary/push-button for switching ON and OFF the
illuminators or the additional reflected-light

illumination and for adjusting the illumination intensity

9 Rotary/push-button for switching ON and OFF the
transmitted-light illumination and for adjusting the
illumination intensity
10 Reflected-light illumination (spot illuminator K LED)
11 Lever for adjusting the transmitted-light illuminator –
bright field or dark field on the stand K EDU
12 Insert plate for placing the specimen

Fig. 3 Microscope system Stemi 305

The illustrated microscope equipment shows an example and may differ from the ones actually

01/2015 435063-7044-001 13

Fig. 6 Stemi 305 MAT microscope set

DESCRIPTION Stereo microscope
ZEISS Microscope sets and fields of application Stemi 305

2.3 Microscope sets and fields of application

Stemi 305 EDU:

− Stemi 305 body in stand K EDU

− integrated controllable vertical illuminator

− reflected-light spot illuminator with height and

angle adjustment, zoomable

− flat transmitted-light unit for bright and dark

field

− for training in tutorial rooms and training

Fig. 4 Stemi 305 EDU microscope set

institutes

Stemi 305 LAB:

Fig. 5 Stemi 305 LAB microscope set

− Stemi 305 body in stand K LAB

− integrated controllable vertical illuminator

− double spot illuminator with height and angle

adjustment

− reflected-light module for bright and dark field

and relief contrast

− hand rest (optional)

− for separation and documentation in the

laboratory

Stemi 305 MAT:

− Stemi 305 ESD body in stand K MAT

− integrated controllable vertical illuminator

− segmentable ring illuminator for reflected light

− for industrial quality assurance and manufacture

− ESD-capable thanks to its antistatic surface

14 435063-7044-001 01/2015

1

Optional mount for eyepiece plates

2

3

Integrated camera adapter 0.5x with

4

Internal thread M52x1.0 to accommodate

5

6

7

8

Guide for mounting a reflected-light spot

9

M8 thread to mount retaining arms (only

Stereo microscope DESCRIPTION
Stemi 305 Interfaces on the microscope body Stemi 305 ZEISS

2.4 Interfaces on the microscope body Stemi 305

∅ 26 mm

Eyepiece tube ∅ 30 mm to replace the
eyepieces

C-mount connector for cameras up to
2/3"(only for Stemi 305 trino)

front optics and other accessories

Mount ∅ 66 mm for ring illuminator

Mount ∅ 76 mm for microscope body

Mount ∅ 84 mm for tables, insert plates

illuminator

stand K)

Thanks to the interface ∅ 76 mm, the Stemi 305
can also be used in conjunction with other stands
of the modular kit of the Stereo system – and
other ZEISS stereo microscopes such as e.g. the
Stemi 508, may also be inserted in the stands K.

Front optics, Stemi 305

− External thread M52x1.0 for fastening in the

zoom body Stemi 305

− Internal thread M49x0.75 to accommodate

optical filters and analyzer for pol.

Fig. 7 Interfaces on the Stemi 305

with stand K LED (schematic diagram)

01/2015 435063-7044-001 15

Fig. 8 Front optics, Stemi 305 (example)

DESCRIPTION Stereo microscope
ZEISS System overview Stemi 305

2.5 System overview

16 435063-7044-001 01/2015

Stereo microscope DESCRIPTION
Stemi 305 System overview ZEISS

01/2015 435063-7044-001 17

Microscope system Stemi 305 EDU

Weight

Microscope system Stemi 305 LAB

Weight

DESCRIPTION Stereo microscope
ZEISS Technical data Stemi 305

2.6 Technical data

Dimensions

Stemi 305 in stand K EDU ................................. 4.6 kg

Dimensions

Stemi 305 in stand K LAB ................................. 6.2 kg

18 435063-7044-001 01/2015

Stereo microscope DESCRIPTION
Stemi 305 Technical data ZEISS

Ambient conditions

Storage (in packaging)

Admissible ambient temperature ...................................................................................... +10 to +40 °C

Admissible relative humidity ........................................................ max. 75 % at +35 °C (non-condensing)

Transport (in packaging)

Admissible ambient temperature ....................................................................................... -40 to +70 °C

Operation

Admissible ambient temperature ...................................................................................... +10 to +40 °C

Admissible relative humidity .................................................................................................. max. 75 %

Atmospheric pressure .............................................................................................800 hPa to 1060 hPa

Pollution degree ................................................................................................................................... 2

Field of application ..............................................................................................................closed rooms

Height of field of application ............................................................................................... max. 2000 m

Operating data – desktop power units, microscope and controller K LED

Safety Class .......................................................................................................................................... II

Type of enclosure ........................................................................................................................... IP 20

Electrical safety .............................................................................. acc. to DIN EN 61010-1 (IEC 61010-1)

taking CSA and UL regulations into account

Pollution degree ................................................................................................................................... 2

Overvoltage category ............................................................................................................................ 2

Line voltage ............................................................................................................. 100 to 240 V ±10 %

Due to the fact that the instrument features a multi-voltage power supply unit, change-over of the device
voltage not required!

Line frequency .................................................................................................................. 50 Hz − 60 Hz

Power consumption: Desktop power unit with connected microscope ..................................... max. 40 VA

Output desktop power unit for microscope

and for Controller K LED ............................................................................................ 12 V DC, max. 2 A

Input desktop power unit for microscope

and for Controller K LED .................................................................... 100-240 V, 50-60 Hz, max. 0.55 A

01/2015 435063-7044-001 19

DESCRIPTION Stereo microscope
ZEISS Technical data Stemi 305

Optical risk group classification acc. to DIN EN 62471:2009

Overall device ....................................................................... LED risk group 2 acc. to DIN EN 62471:2009

Integrated vertical illumination .............................................. LED risk group 2 acc. to DIN EN 62471:2009

Spot illuminator K LED (reflected illumination) ....................... LED risk group 2 acc. to DIN EN 62471:2009

Double spot illuminator K LED (reflected illumination) ............ LED risk group 2 acc. to DIN EN 62471:2009

Transmitted-light unit in stand K LAB .................................... LED risk group 2 acc. to DIN EN 62471:2009

Transmitted-light unit in stand K EDU ................................... LED risk group 2 acc. to DIN EN 62471:2009

LED reflected illumination, peak at 460 nm, .......................... LED risk group 2 acc. to DIN EN 62471:2009

LED transmitted illumination, peak at 465 nm, ...................... LED risk group 2 acc. to DIN EN 62471:2009

20 435063-7044-001 01/2015

Notes on Instrument

For installation and/or transport of the microscope or the stand, exclusively use the handle

Stereo microscope INSTALLATION
Stemi 305 General information ZEISS

3 INSTALLATION

3.1 General information

Before installing and commissioning the device, make sure to read the
Safety carefully (see Section 1.2, Page 8).

The Stemi 305 with the necessary tools and optional equipment is delivered in several standard packages.

• Take all units out of the packaging and check them for completeness according to the delivery note.

(Fig. 3/5) provided to this effect.

• Remove transport securing devices (adhesive tapes etc.).

• Keep original packaging for a possibly extended period of storage or return of the device to the

manufacturer or dispose of it appropriately.

3.2 Installing the stereo microscope

• Place stand on a level, firm support.

• If the Stemi 305 body (Fig. 10/2) has not yet

been mounted, insert it in the Stemi mount
(Fig. 10/4), align it and secure it using the
clamping screw (Fig. 10/6).
The appropriate Allen key (Fig. 10/9) is located
in the support on the back of the device.

• If the two eyepieces (Fig. 10/1) have not yet

been mounted, insert them both to their stop in
the eyepiece tube.

• Remove the two protective caps from the

eyepieces (Fig. 9/1) and remove the protective
cap (Fig. 9/2) from the objective by turning.

Fig. 9 Removing caps

01/2015 435063-7044-001 21

tor has been described in

focusing drive can be set as required

case basis by adjusting

INSTALLATION Stereo microscope
ZEISS Installing the stereo microscope Stemi 305

• Insert the connection cable (Fig. 10/3) supplied

with the device into the socket on the Stemi
body and in the socket on the Stemi mount.

• Insert glass plate or B/W plastic plate (Fig. 10/7)

in the mount in the base of the stand.
To this effect, press the glass plate or the B/W
plastic plate against the spring (Fig. 10/8) and
make it lock in.

Installation of an additional reflected­light illumina
Section 3.3.1 on page 23.

Fig. 10 Installing the stereo microscope

If necessary, the ease of motion of the

on a case-by­the two focusing buttons (Fig. 11/1
and 2) in opposite directions.

The ease of motion must not be set so
loosely that the drive moves down­wards by itself. This might damage the
microscope or the specimen.

Fig. 11 Setting the ease of motion of the

focusing drive

22 435063-7044-001 01/2015

Stereo microscope INSTALLATION
Stemi 305 Mounting optional components ZEISS

3.3 Mounting optional components

3.3.1 Mounting additional reflected-light illuminator

Various reflected-light illuminators can be used, depending on the case of application.

For fastening, defined interfaces have been provided on the microscope.

3.3.1.1 Mounting spot illuminator K LED or

double spot illuminator K LED

• Move the microscope body (Fig. 12/1) into its

uppermost position via the focusing knob
(Fig. 12/2).

• Insert the spot illuminator (Fig. 12/7) in the

stand by sliding the holder (Fig. 12/5) into the
stand guide (Fig. 12/4) from below and push it
upwards into the required position.

• Plug the connection cable (Fig. 12/6) of the spot

illuminator into the lower socket of the Stemi
mount (Fig. 12/3).

The double spot illuminator is
mounted in an analogical manner.

Fig. 12 Mounting spot illuminator

01/2015 435063-7044-001 23

The best clamping effect is achieved if

) of the adapter and

) of the

INSTALLATION Stereo microscope
ZEISS Mounting optional components Stemi 305

3.3.1.2 Mounting ring illuminator K LED (segmentable)

Mounting ring illuminator to microscope body

• Insert the connection cable (Fig. 13/1) of the

ring illuminator (Fig. 13/4) into the lower socket
(Fig. 13/2) of the Stemi mount and into the
socket of the ring illuminator.

• Push ring illuminator onto the microscope body

(Fig. 13/3) from below, and secure using the
knurled screw (Fig. 13/5).

Fig. 13 Mounting ring illuminator on

microscope body

Mounting ring illuminator to front optics 3

• Put the slotted adapter (Fig. 14/4) supplied with

the device (OD 66 mm, ID 58 mm) onto the
front optics (Fig. 14/1) from below. The
circumferential bead (Fig. 14/3) inside the
adapter must lock into the outer groove
(Fig. 14/2) of the front optics.

• Push ring illuminator (Fig. 14/6) onto the AD of

the adapter and secure it by means of clamping
screw (Fig. 14/7).

the slot (Fig. 14/5
the clamping screw (Fig. 14/7
illuminator are offset by approx. 90°.

Fig. 14 Mounting ring illuminator to front

optics 3

24 435063-7044-001 01/2015

To this effect, refer also to the

ating instructions

s are inserted by the

customer, it must be ensured that the

Stereo microscope INSTALLATION
Stemi 305 Mounting optional components ZEISS

3.3.1.3 External fiber-optic illumination

• Switch on external fiber-optic illumination

(Fig. 15/1), adjust illumination intensity and set
illumination by bending the two goose necks
(Fig. 15/2) so that the specimen is optimally lit.

separate oper
regarding the cold light source.

3.3.1.4 Placing reversible eyepiece cup onto the eyepiece

The eyepieces feature rubber protection rings for spectacles to avoid the spectacles being scratched.
These rings may be replaced at choice by reversible eye cups (Fig. 16/1).

Fig. 15 Mounting external fiber-optic

illumination

• To this effect remove the protection rings for spectacles (Fig. 16/2) from the eyepieces and position

the eye cups (Fig. 16/1).

3.3.1.5 Insert the eyepiece plate into the eyepiece.

The adjustable eyepieces are intended for use with
eyepiece plates.

• Unscrew stop (Fig. 16/6) out of the eyepiece

(Fig. 16/3).

• Pull locking ring (Fig. 16/4) out.

• Insert eyepiece plate (Fig. 16/5).

• Insert locking ring and re-fasten stop.

If eyepiece plate

writing is mirror-inverted before inser­tion into the eyepiece, and true to side
after insertion.

Fine cotton gloves should be worn to insert or replace eyepiece plates.

01/2015 435063-7044-001 25

Fig. 16 Inserting the eyepiece plate

Safe disconnection from the power supply is ensured exclusively by removing the mains plug.

Do not replace detachable mains cables by inadequately designed mains cables. Only the

INSTALLATION Stereo microscope
ZEISS Connecting stereo microscope to the power supply Stemi 305

The slight image offset which is due to the additional glass distance is taken into account on the diopter
scale by the zero position not being indicated by the white dot (Fig. 16/W), but by a red dot (Fig. 16/R).

Eyepieces with pre-inserted eyepiece plates are available directly from ZEISS.

3.4 Connecting stereo microscope to the power supply

• Insert the mains cable (Fig. 17/2) in the mains

supply socket (Fig. 17/1) of the stand.

• Connect the mains cable (Fig. 17/2) to a mains

supply socket outlet.

Fig. 17 Connecting the stereo microscope

The switch on the stereo microscope only switches into standby mode.

specified power supply lines may be used.

3.5 Switching stereo microscope ON or OFF

• Use the power switch (Fig. 17/3) to switch the

stereo microscope ON or OFF.

26 435063-7044-001 01/2015

The functionality of the knob for the

light illuminator has been

"0" on white dot (without eyepiece
plate). "0" on red dot (with eyepiece

Check that the eyepieces are inserted

To this effect, a distance of approx.

between the

Stereo microscope OPERATION
Stemi 305 Adjusting the stereo microscope ZEISS

4 OPERATION

4.1 Adjusting the stereo microscope

The stereo microscope has been connected and
switched ON.

1. Place specimen (Fig. 18/7) centrally on the
glass or plastic plate (Fig. 18/6) and illuminate
it (Fig. 18/5).

reflected­described in Section 4.2 on page 28.

2. Set diopter compensation on the adjustable
eyepieces (Fig. 18/1) to "0".

plate), see Fig. 19.

into the tube to their stop.

3. Set individual eye distance by shifting the

Fig. 18 Adjusting the stereo microscope

eyepiece tube laterally (Fig. 18/2), until only
one complete light circle (specimen field) is

visible when looking into the eyepieces with
both eyes.

2 cm must be maintained
eye and the eyepiece.

4. Initially, set the zoom (Fig. 18/3) to the
smallest magnification.

5. Focus on a small distinct detail in the center of
the specimen (Fig. 18/4).

Fig. 19 Adjusting the eyepieces

6. Adjust maximum zoom value (Fig. 18/3). Magnification will probably make the distinct detail appear

blurred and no longer in the image center.

7. Sharpen the specimen by focusing, then shift the specimen to re-search for the detail and focus
"sharply" again (Fig. 18/4).

8. Afterwards, reset to the lowest zoom value and correct any image blurring for each eye separately by
diopter compensation on the adjustable eyepieces (not on the focusing knob, Fig. 18/4)
(compensation of visual defect).

After adjustment of the stereo microscope, the image focus remains unchanged over the entire zoom
range.

After change of the observer, repeat steps 3. to 8.

01/2015 435063-7044-001 27

light illuminators, the intensity of the additionally installed

spot illuminator, ring illuminator, etc.) is

OPERATION Stereo microscope
ZEISS Setting reflected-light illumination Stemi 305

4.2 Setting reflected-light illumination

4.2.1 Integrated vertical illuminator

Each Stemi 305 body features a vertical illuminator
of its own.

If no second reflected-light illumination has been
installed, the vertical illuminator is operated as
described below:

• Press the knob (Fig. 20/1) once to switch the

vertical illuminator (Fig. 20/2) alternately ON or
OFF.

• Turning of the knob serves to adjust the

illumination intensity.

If a second reflected-light illumination has been
installed, the reflected-light illuminators are
operated as described below:

Fig. 20 Setting the vertical illuminator

When mixing both reflected­reflected-light illuminator (spot illuminator, double­adopted. In this case, only the vertical illumination is adjusted by turning the knob.
Now, the required intensity of the vertical light is blended in with the reflected-light
illuminator.

• Press the knob successively to set four

illumination states:

1. Press: to switch the vertical illumination
ON.

2. Press: to switch the second reflected-light
illuminator ON (vertical illumination
OFF).

3. Press: to switch both reflected-light
illuminators ON.

4. Press: to switch both reflected-light
illuminators OFF.

• By turning the knob, the illumination intensity

of the switched-ON reflected-light illuminator
can be adjusted.

28 435063-7044-001 01/2015

In the lower positions, an oblique light

For switching the illumination intensity
ON and OFF and adjusting the

For switching the illumination intensity
ON and OFF and adjusting the

Here again, an oblique light effect can
be produced in the lower positions with

The spot illuminator and the double spot illuminator have a thread M24 for placing

By turning the spot polarizers, overexposure reflexes on the specimen can be reduced.

Stereo microscope OPERATION
Stemi 305 Setting reflected-light illumination ZEISS

4.2.2 Spot illuminator

• Push spot illuminator in the guide to the re-

quired height (Fig. 21/2).

effect is created to produce cast sha­dows for enhancing surface structures.

• Set the angle (Fig. 21/1) via the pivot of the

spot illuminator so that the object is optimally
illuminated.

• Set size and brightness of the spot by axially

shifting the focusing unit (Fig. 21/3)
(illumination zoom).

illumination intensity refer to Section

4.2.1, page 28.

Fig. 21 Setting the spot illuminator

4.2.3 Double spot illuminator

• Move the microscope body to its uppermost

position via the focusing knob (Fig. 22/1). Push
double spot illuminator (Fig. 22/2) to the
required level within the guide.

• Bend the two goose necks (Fig. 22/3) to adjust

the spots so that the specimen is optimally
illuminated.

illumination intensity refer to Section

4.2.1, page 28.

the goose neck set horizontally.

Fig. 22 Double spot illuminator

polarization filters in front. The appropriate analyzer is screw-fastened in the M52x1.0 thread
of the microscope body (Fig. 7/4).

01/2015 435063-7044-001 29

1. Press: full circle

OPERATION Stereo microscope
ZEISS Setting reflected-light illumination Stemi 305

4.2.4 Ring illuminator

• By pressing the front button (Fig. 23/2)

successively, the user can change over between
four illumination states of the ring illuminator
(Fig. 23/3):

2. Press: semi-circle

3. Press: quadrant

4. Press: two quadrants
(opposite each other)

• When switching the semi-circle or quadrant

illumination ON, the specimen is first
illuminated from the rear (from the direction of
the stand column).

• By pressing the rear button once briefly

(Fig. 23/1), the adjusted segment is turned

Fig. 23 Ring illuminator

forward clockwise by one quarter turn in each
case.

• By pressing and keeping the rear button

(Fig. 23/1) pressed for over 2 seconds, the
adjusted segment is set into continuous rotation
(rotation steps of 1/8 circle, 1 second per turn in
each case).

• By pressing the rear button once more (Fig. 23/1), rotation is stopped and the specimen is backlit

again.

For switching the illumination intensity ON and OFF and adjusting the illumination intensity
refer to Section 4.2.1, page 28.

30 435063-7044-001 01/2015

Stereo microscope OPERATION
Stemi 305 Setting reflected-light illumination ZEISS

4.2.5 Controller K LED

The controller K (Fig. 24/4) is used to activate the
vertical illumination of the Stemi 305 body or the
ring illuminator in case of applications with boom
stands A or U or additional stands.

• Mount the stands A and U including the

fastening of the Stemi 305 body to the Stemi
mount as described in the operating
instructions for the boom stands.

• Screw-fasten front lens system 0.5x/185 mm

(Fig. 24/9) to the microscope body.

• When using the ring illuminator, adapt the

latter to the front lens system (Fig. 24/9) using
the adapter supplied with the device (ID
58 mm, OD 66 mm).

• Secure the adhesive side of the magnetic plate

(Fig. 24/7) to the swivel arm stand.

• Plug the connection cable supplied with the

device (1 m long) (Fig. 24/2) into the socket
(Fig. 24/8) on the microscope body or the ring
illuminator and into the socket (Fig. 24/3) on
the back of the Controller K (Fig. 24/4).

Fig. 24 Controller K LED

• Place Controller K (Fig. 24/4) next to the

magnetic plate (Fig. 24/7).

• Connect Controller K to the power supply using the power unit (Fig. 24/1).

• Align swivel arm stand (Fig. 24/6) to enable an ergonomic working position.

• Turn the knob (Fig. 24/5) on the Controller K to switch the vertical illuminator (or the ring illuminator)

ON and adjust illumination intensity by turning the knob.

• Place specimen in the illuminated specimen field.

• Set the stereo microscope to its initial setting, see Section 4.1 on page 27.

01/2015 435063-7044-001 31

Press the upper knob (Fig. 25/1) to blend

OPERATION Stereo microscope
ZEISS Transmitted-light illuminators Stemi 305

4.3 Transmitted-light illuminators

4.3.1 Transmitted-light module in stand K EDU

• Switch transmitted-light illumination ON by

pressing the lower knob (Fig. 25/2).

• With the transmitted light ON, turn this knob to

adjust the illumination intensity.

• Change over by adjusting the lever (Fig. 25/3)

between the bright field and the dark field
illumination.

− Front position: Dark field (DF)

− Rear position: Bright field (BF)

in reflected light (see Section 4.2 on
page 28).

Fig. 25 Transmitted-light module in

stand K EDU

32 435063-7044-001 01/2015

Clear mirror for high-contrast bright

By pushing the sliding knob (Fig. 26/2)
into a central position and by tilting the
mirror, oblique light effects (RC) are
created which are very well suited to

Stereo microscope OPERATION
Stemi 305 Transmitted-light illuminators ZEISS

4.3.2 Transmitted-light unit in stand K LAB

• Switch transmitted-light illumination ON by

pressing the lower knob (Fig. 26/1).

• With the transmitted light ON, turn this knob to

adjust the illumination intensity.

• Adjust the bright field (BF) by pushing the

sliding knob (Fig. 26/2) into its rear position
(BF).

• Turn the sliding knob (Fig. 26/2) to swing the

clear or diffuse mirror into position or to adjust
the inclination angle.

field, diffuse mirror for homogenous
bright field.

• Change over to dark field illumination (DF) by

pushing the sliding knob (Fig. 26/2) into its
front position.

• Turn the mirror until the specimen structures

are brightly lit against the dark background.

Fig. 26 Transmitted-light unit in stand K LAB

display non-coloured specimens.

01/2015 435063-7044-001 33

The devices are not equipped with special equipment protecting them from corrosive,

ments, in particular the relevant

rom the mains power before cleaning. Make sure, that no cleaning

CARE, MAINTENANCE AND SERVICE Stereo microscope
ZEISS Care Stemi 305

5 CARE, MAINTENANCE AND SERVICE

5.1 Care

Care of the devices is restricted to the following operations:

potentially infectious, toxic and radioactive or other samples that may be hazardous to health. If
you handle such samples, be sure to observe all legal require
national accident prevention regulations.

• Remove contamination in accordance with the national accident prevention regulations.

• Disconnect the devices from the mains power after use. Protect the devices from dust and moisture by

using a suitable cover (protective device cover).

• Never expose the devices to inadmissible climate conditions (high humidity and temperature).

Disconnect the devices f
fluids penetrate into the device.

Tenacious impurities on glass surfaces such as fingerprints and traces of grease, are best removed using a
cotton wool bud wound around a round wooden stick and very little distilled water or non-aggressive
solvent:

• Distilled water: Clean glass surface with the slightly moistened cotton wool bud from the center to the
edge, using circular movements.

• Cleaning solution for optics, consisting of 15 % isopropanol and 85 % surgical spirit (gasoline): Clean
glass surface by the slightly moistened cotton wool bud from the center to the edge, using circular
movements.

• Remove dust from optical surfaces using a brush with natural bristles or blow using rubber bellows (air
blower).

• Clean plastic parts using commercially available cleaning products (no solvents!).
Tenacious impurities can be treated carefully with benzine or white spirit.

• All labels on the components and on the desktop power unit may only be cleaned using a dry cotton
cloth.

34 435063-7044-001 01/2015

Only the desktop power unit specified

plug is

removed from the socket before the

Stereo microscope CARE, MAINTENANCE AND SERVICE
Stemi 305 Maintenance ZEISS

5.2 Maintenance

5.2.1 Replacing the power unit

by the manufacturer may be used.

Make sure that the mains

instrument is opened.

• Remove the mains plug of the power cable

from the socket.

• Remove the power cable from the back of the

device.

• Use an Allen key to release the screw (Fig. 27/1)

on the underside of the housing cover.

• Pull off housing cover (Fig. 27/2) slightly at the

bottom, then press it upwards.

• Release the plug (Fig. 27/3) and remove the

faulty desktop power unit (Fig. 27/4).

• Insert the new desktop power unit and connect

the plug.

• Press the upper part of the housing cover into

place and push housing cover down.

• Secure housing cover by means of the screw.

• Insert the power cable on the back of the

device.

• Insert the mains plug into a mains supply

socket.

Fig. 27 Changing the power unit

01/2015 435063-7044-001 35

To clean the LED board, use a soft

CARE, MAINTENANCE AND SERVICE Stereo microscope
ZEISS Maintenance Stemi 305

5.2.2 Opening the stand cover plate, stand K EDU

The stand cover plate (Fig. 28/1) may be removed
for cleaning or to remove any foreign matter which
might fallen in inadvertently.

• To this effect, release the three recessed head

screws (Fig. 28/2), lift off the stand cover plate
(Fig. 28/1) carefully and then put it aside.

• Make sure that the cable is not disconnected

from the stand cover plate (Fig. 28/1).

lintfree cloth, a cotton wool bud wound
around a round wooden stick or a brush
with natural bristles, possibly moistened
slightly in distilled water.

Fig. 28 Opening the stand cover plate,

stand K EDU

36 435063-7044-001 01/2015

Make sure that the cable (Fig. 29/4) is

To clean the LED, the optical elements

free cloth,
a cotton wool bud wound around a
round wooden stick or a natural hair
brush, possibly moistened slightly in

Stereo microscope CARE, MAINTENANCE AND SERVICE
Stemi 305 Maintenance ZEISS

5.2.3 Opening the stand cover plate, stand K LAB

The transmitted-light base (Fig. 29/1) can be
removed from the stand cover plate (Fig. 29/3) for
cleaning or for removal of any foreign matter
which might have fallen in inadvertently.

• Release the three screws (Fig. 29/2) from the

stand cover plate (Fig. 29/1) using an Allen key
SW 2.5 and put them aside.

• Carefully lift the stand cover plate (Fig. 29/3) off

the transmitted-light base (Fig. 29/1) and put
the stand aside.

not disconnected from the transmitted­light base (Fig. 29/1).

and the mirror, use a soft lint

distilled water.

01/2015 435063-7044-001 37

Fig. 29 Opening the stand cover plate,

stand K LAB

CARE, MAINTENANCE AND SERVICE Stereo microscope
ZEISS Service Stemi 305

5.2.4 Wearing parts

The following wearing parts can be ordered directly from ZEISS:

Description Order No. Remark

Transparent glass plate, d = 84 mm 435425-9310-000

S/W plastic plate, d = 84 mm 435425-9320-000

Eyepiece PL 16x/16 Br foc. 444054-9000-000

Eyepiece cup 444801-0000-000 required 2x

Eyepiece 16x/14 Br foc. 435063-9160-000

Eyepiece W 25x/10 foc. 455046-9901-000

Set of small components
consisting of:

- 2x spiral cables RJ 12

- 1x cable RJ 12, length 1 m

- 2x caps, eyepiece

- 1x cap, microscope body

- 1x Allen key, SW 3

- 1x M6 clamping screw, microscope mount

- 2x M6 clamping screws for C mount

- 2x rubber rings, eyepiece

Protective dust cover, stand K 415500-1800-000

Cover, C-mount Stemi 305 trino

5.3 Service

All interventions in mechanical, optical and electronic internal stereo microscope components may only
be performed by Carl Zeiss Service or by specifically authorized qualified staff.

To ensure your stereo microscope is optimally set and also operates correctly over an extended period of
time, we recommend that you conclude a service/maintenance contract with ZEISS.

For reordering or in case of service, contact your nearest regional ZEISS representative.

38 435063-7044-001 01/2015

Stereo microscope CARE, MAINTENANCE AND SERVICE
Stemi 305 Product disposal ZEISS

5.4 Product disposal

The product was developed, tested and produced in accordance with the valid regulations and guidelines
for environmental law of the European Union.

The product and its accessories comply with the EU-regulations 2011/65/EC (RoHS) and 2012/19/EC
(WEEE), and the German Electrical and Electronic Equipment Act (ElektroG).

The product contains electronic components which must be disposed of according to the requirements of
the 2002/19/EC WEEE Directive and not as domestic waste. In addition, the national regulations must be
complied with.

For details on disposal and recycling please refer to your relevant ZEISS sales or service organization.

01/2015 435063-7044-001 39

APPENDIX Stereo microscope
ZEISS List of abbreviations Stemi 305

6 APPENDIX

6.1 List of abbreviations

BF Bright field
CL Cold Light
DIN Deutsche Industrie-Norm (German Industrial Standard)
DF Dark field
EN Europäische Norm (European Standard)
IEC International Electrotechnical Commission
IP Internal Protection (Type of enclosure)
IvD In-vitro diagnostics
LED Light Emitting Diode
RC Relief contrast (oblique light)
S/W Schwarz/weiß (black / white)
UV Ultra-violet

40 435063-7044-001 01/2015

Stereo microscope APPENDIX
Stemi 305 Troubleshooting ZEISS

6.2 Troubleshooting

Description of fault Cause of error Troubleshooting

Stereo microscope illumination
cannot be switched ON.

Vertical reflected-light illumination is
not lit.

Additional reflected-light
illuminators are not lit.

Transmitted-light illuminator does
not light up

Power supply interrupted.
Power switch not switched ON

Power unit faulty. Replacing the power unit, see

Power supply cable RJ 12 not
plugged in.

Power supply cable RJ 12 not
plugged in.

Reflected light not switched ON or
dimmed.

Transmitted light not switched ON
or dimmed.

Transmitted light not connected. Open stand K EDU or K LAB and

Check or establish connection to
power supply. Switch power switch
on back of stereo microscope ON.

Section 5.2 on page 35

Insert RJ plug correctly, see Section

3.2 or 3.3 on pages 21 and 23

Insert RJ plug correctly, see Section

3.2 or 3.3 on pages 21 and 23

Press and turn knob Fig
several times to switch reflected
light ON and to increase its intensity;
see Section 4.2 on page 28

Press and turn rotary / push-button

. 25/2 to switch illumination ON

Fig
and to increase its intensity; see
Section 4.3 on page 32

according to Section 5.2.3 on
page 37

connect the plug-in connectors of
the LED illumination.

. 20/1

Transmitted-light illuminator dirty or
control lever cannot be actuated.

Microscope mount moves down by
itself.

Liquids or foreign matter in the
transmitted-light unit.

Focusing drive too easy to move. Set ease of motion of the focusing

Open and clean stand K EDU or K
LAB and eliminate foreign matter
according to Section 5.2.3 on
page 37

drive, see Section 3.2 on page 21

Should any other faults occur or should you have queries regarding troubleshooting, please contact your
regional ZEISS representative.

01/2015 435063-7044-001 41

APPENDIX Stereo microscope
ZEISS List of illustrations Stemi 305

6.3 List of illustrations

Fig. 1 Warning and information labels on the device................................................................... 11

Fig. 2 Warning labels and apertures for LED radiation .................................................................. 11

Fig. 3 Microscope system Stemi 305 ............................................................................................ 13

Fig. 4 Stemi 305 EDU microscope set .......................................................................................... 14

Fig. 5 Stemi 305 LAB microscope set ........................................................................................... 14

Fig. 6 Stemi 305 MAT microscope set ......................................................................................... 14

Fig. 7 Interfaces on the Stemi 305 with stand K LED (schematic diagram) ..................................... 15

Fig. 8 Front optics, Stemi 305 (example) ...................................................................................... 15

Fig. 9 Removing caps .................................................................................................................. 21

Fig. 10 Installing the stereo microscope ......................................................................................... 22

Fig. 11 Setting the ease of motion of the focusing drive ................................................................ 22

Fig. 12 Mounting spot illuminator ................................................................................................. 23

Fig. 13 Mounting ring illuminator on microscope body .................................................................. 24

Fig. 14 Mounting ring illuminator to front optics 3 ........................................................................ 24

Fig. 15 Mounting external fiber-optic illumination ......................................................................... 25

Fig. 16 Inserting the eyepiece plate ............................................................................................... 25

Fig. 17 Connecting the stereo microscope ..................................................................................... 26

Fig. 18 Adjusting the stereo microscope ........................................................................................ 27

Fig. 19 Adjusting the eyepieces ..................................................................................................... 27

Fig. 20 Setting the vertical illuminator ........................................................................................... 28

Fig. 21 Setting the spot illuminator ............................................................................................... 29

Fig. 22 Double spot illuminator ..................................................................................................... 29

Fig. 23 Ring illuminator ................................................................................................................. 30

Fig. 24 Controller K LED ............................................................................................................... 31

Fig. 25 Transmitted-light module in stand K EDU .......................................................................... 32

Fig. 26 Transmitted-light unit in stand K LAB ................................................................................. 33

Fig. 27 Changing the power unit .................................................................................................. 35

Fig. 28 Opening the stand cover plate, stand K EDU ..................................................................... 36

Fig. 29 Opening the stand cover plate, stand K LAB ....................................................................... 37

42 435063-7044-001 01/2015

Stereo microscope APPENDIX
Stemi 305 Index ZEISS

6.4 Index

A

Adjusting the stereo microscope .................... 27

Ambient Conditions ....................................... 19

Ambient temperature .................................... 19

Apertures for LED radiation ............................ 11

B

Bright field .................................................... 32

C

Care .............................................................. 34

Compensation of visual defect ....................... 27

Connecting ................................................... 26

Contents ......................................................... 5

Controller K LED ............................................ 31

Copyright ........................................................ 4

D

Dark field ...................................................... 32

Description .................................................... 13

Dimensions .................................................... 18

Diopter compensation ................................... 27

Double spot illuminator.................................. 23

E

Ease of motion of the focusing drive .............. 22

Eyepiece ........................................................ 27

Eyepiece cup ................................................. 25

Eyepiece plate ............................................... 25

F

M

Maintenance ................................................. 35

Microscope system ........................................ 13

Mixed light .................................................... 28

O

Operation ..................................................... 27

P

Product disposal ............................................ 39

Protection ring for spectacles ......................... 25

R

Reflected-light illumination ............................ 23

Ring illuminator ............................................. 24

S

Safety ............................................................. 8

Service .......................................................... 38

Setting

Reflected-light illumination ......................... 28

Stereo microscope ..................................... 27

Transmitted-light illumination ..................... 32

Setting reflected-light illumination ................. 28

Setting transmitted-light illumination ............. 32

Spot illuminator ............................................. 23

Switching OFF ............................................... 26

Switching ON ................................................ 26

Swivel arm stand

........................................... 31

System overview ............................................ 16

Fiber-optic illumination .................................. 25

Front optics 3 ................................................ 24

I

Illumination intensity ................................ 29, 30

Illumination state of the ring illuminator ......... 30

Index ............................................................. 43

Indication for use ........................................... 13

Information labels .......................................... 11

Information, general .................................. 7, 21

Installation..................................................... 21

Instrument safety ............................................. 8

Interfaces ...................................................... 15

Introduction .................................................... 7

L

LED class ......................................................... 8

T

Technical data ............................................... 18

Transmitted-light module in stand K EDU ....... 32

Transmitted-light unit in stand K LAB ............. 33

Troubleshooting ............................................ 41

U

Use ............................................................... 13

V

Vertical illumination ....................................... 28

W

Warning labels .............................................. 11

Warranty notes ............................................. 12

Wearing parts ............................................... 38

Weight .......................................................... 18

01/2015 435063-7044-001 43