Zeiss LSM 510 Service Manual

DuoScan

DuoScan

March 2006

LSM 510 / LSM 510

LSM 510 META / LSM 510 META

Release 4.0

INTRODUCTION LSM 510 DuoScan
Carl Zeiss LSM 510 META DuoScan

Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning the
safe operation of the instrument.

The specifications are subject to change; the manual is not covered by an update service.

© Unless expressly authorized, forwarding and duplication of this document, and the utilization and

communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.

All rights reserved in the event of granting of patents or registration of a utility model.

Developed in
Collaboration with European Molecular Biology Laboratory (EMBL)
PF 102209
Meyerhofstr. 1
69012 Heidelberg
GERMANY
Phone: ++49-6221-387-0
Telefax: ++49-6221-387-306

Issued by Carl Zeiss MicroImaging GmbH

07740 Jena, Germany
Phone: [phone]
Fax: [phone]
E-mail: [e-mail]

www.zeiss.de/lsm

II B 45-0021 e 03/06

LSM 510 INTRODUCTION
LSM 510 META Carl Zeiss

How to make best use of the LSM 510 and LSM 510 META operating instructions:

This operating manual also includes the instructions for the LSM 510 META DuoScan and the
LSM 510 DuoScan systems.

A few symbols in these operating instructions will help you to recognize the nature and purpose of
information immediately:

The WARNING symbol warns against hazards for the user that might arise when operating the
laser.

This WARNING symbol warns against hazards from dangerously high voltages.

The CAUTION symbol warns against faults and hazards that might arise during operation and
which might cause damage to the unit.

The NOTE symbol will help you to optimally solve your work problem. It represents a practical
tip which will help you to find out which settings and methods are capable of improving or
accelerating a procedure.

The HOT SURFACE symbol warns against hazards for the user that might arise when touching
the lamp housing during operation.

The MAINS PLUG symbol remembers service personal to pull the mains plug before opening the
device housing.

Depending on the problem, these operating instructions will supply you with various possibilities:

• If you want to know where to find certain general areas of information, refer to the following outline

of sections to get a general overview.

• You will find a detailed table of contents at the start of every chapter. There you will see at a glance

what topics are dealt with in detail.

Always remember: The time you invest in getting acquainted with the product will pay

for itself many times over in your application task.

03/06 B 45-0021 e III

INTRODUCTION LSM 510 DuoScan
Carl Zeiss LSM 510 META DuoScan

Contents

1

2

3

4

5

Notes on Device Safety

This section contains general notes on device safety, safe operation, and possible hazards
caused by failure to observe the instructions.

LSM 510 and LSM 510 META - Setup Requirements

The Setup Requirements section outlines the installation and supply requirements of the
LSM 510 and LSM 510 META Microscope Systems, together with the relevant specifications.

Introduction to Laser Scanning Microscopy

Here you will find an introduction to Laser Scanning Microscopy, with an explanation of the
principles of confocal imaging. The section also outlines the ways to present LSM image series
in three dimensions, and introduces you to the performance features of your LSM 510 or
LSM 510 META.

Operation in Expert Mode

In the Operation section you will find the most important steps and procedures of the
LSM menu structure. The step-by-step description how to get an image will be shown by
typical application examples including the WINDOWS XP graphic user environment.

VBA Programming for LSM

6

7

8

9

10

11

Routine Mode and Tools

This section contains a description of the Routine Mode for scanning images using the
LSM scanning module and the use of the tools for setting the microscope.

3D for LSM 510 and LSM 510 META

This section contains a description of the LSM 3D software package (basic program and add­ons). At the same time, all functions and settings are presented in a systematic form and in the
order in which they can be reached from the basic menu via sub-menus and dialog boxes.

Annex

The annex contains the Application-specific Configurations, special notes and information for
using the LSM microscope and the brochure The confocal Laser Scanning Microscopy.

Multiphoton Laser Scanning Microscopy - Using the Zeiss LSM 510 META NLO

Certification

Brief Operating Manual

This section contains the brief instructions of the LSM 510 and LSM 510 META Microscope
Systems.

12

IV B 45-0021 e 03/06

Laser safety warning labels

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META General Carl Zeiss

CHAPTER 1 NOTES ON DEVICE SAFETY

CONTENTS

Page

1

NOTES ON DEVICE SAFETY .............................................................................................1-2

1.1 General..............................................................................................................................1-2

1.2 Warning and Information Labels ........................................................................................1-3

1.3 Regulations ......................................................................................................................1-11

1.4 Notes on Setting up the Microscope System.....................................................................1-12

1.5 Power Requirements ........................................................................................................1-14

1.6 Notes on Handling the Laser Components........................................................................1-15

1.7 Physical Dimensions .........................................................................................................1-17

1.8 Environmental Requirements............................................................................................1-17

1.9 Notes on Handling the Computer and Data Media ...........................................................1-18

1.10 Notes on Care, Maintenance and Service .........................................................................1-19

1.11 User Interface...................................................................................................................1-20

1.11.1 Mounting and Dismounting Lamps, TPMT and Switching Mirror ......................................1-20

1.11.2 Mounting and Dismounting the Scan Head......................................................................1-21

03/06 B 45-0021 e 1-1

NOTES ON DEVICE SAFETY LSM 510 DuoScan Carl Zeiss General LSM 510 META DuoScan

1 NOTES ON DEVICE SAFETY

1.1 General

The LSM 510 and LSM 510 META laser scanning microscope, including its original accessories and
compatible accessories from other manufacturers, may only be used for the purpose of microscopic
techniques.

Laser Scanning Microscopes (LSM) are intended for high resolution imaging of biological or material
samples, whereby in contrast to wide field microscopy the specimen is illuminated raster-fashion with a
focused laser beam and the optical arrangement prevents light from out-of-focus regions of the
specimen contributing to image formation.

Installation and commissioning of the LSM 510 and LSM 510 META system must be performed
by authorized Carl Zeiss service staff. The system should not be used prior to instruction by a
Carl Zeiss representative.

The manufacturer will not assume liability for any malfunction or damage caused by anything
other than the intended use of the LSM 510 or LSM 510 META or individual modules or parts
of it, nor by any repair or other service operation performed or attempted by persons other
than duly authorized service staff. Any such action will invalidate any claim under warranty,
including parts not directly affected by such action. This also includes the modification of the
system computer with new cards, etc. by the user. The use of a camera at the base port of
Axiovert 200 M Combi stands is not allowed for reasons of laser safety. Any manipulation will
result in the loss of warranty of laser safety.

Please read also the notes on device safety and manuals of the microscope, the HBO, the HAL and
additional optional devices, if ordered, as the UV Laser, the piezo focusing device, the heating inserts and
the Ti:Sa Laser.

⇒ As the system is largely operated via menus on a computer, you should be familiar with the

principles of the operating system and its WINDOWS, WINDOWS 2000 or Windows XP graphical
user interface. The respective manuals are supplied together with the programs.

The LSM 510 and LSM 510 META are devices that belong to laser hazard class 3B. The systems
are equipped with safety interlocks that comply with laser hazard class 3B and 4. If equipped
with a Ti:Sa Laser (see list in section

1.6), the LSM 510 and LSM 510 META are devices that
belong to laser hazard class 4. WHO recommendations concerning health and industrial
protection when handling laser devices must be observed. The operator of the unit must also
observe all and any relevant statutory accident prevention regulations. The user is referred to
the safety data sheet provided together with the manual.

1-2 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Warning and Information Labels Carl Zeiss

1.2 Warning and Information Labels

The warning and information labels attached on the LSM 510 and LSM 510 META must be
observed. Check whether all of the labels shown below are provided on your instrument, and
contact Carl Zeiss Germany or one of the service agencies if you should discover that any of the
labels should be missing. You will receive a free replacement.

Description of labels

Caution: Faults and hazards that might arise during operation which might cause damage to
the unit or injury to the user.

Attention: Laser irradiation hazards possible when operating the system.

Attention: High voltage.

Pull the mains plug before opening the device housing.

Caution:

Hot surface.

Caution: UV radiation.

Caution: Fingers can be caught.

The arrow points to the opening where laser light comes out during operation of the system.

Other labels on the system include one of the above depicted symbols and a detailed
description of the handling instructions. See also the following drawings of the system parts.

03/06 B 45-0021 e 1-3

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Warning and Information Labels LSM 510 META DuoScan

1:1

ZERO

FOCUS

OBJECTIVE

REFLECTOR

Fig. 1-1 Warning and information labels on the Axiovert 200 M microscope with the LSM 510 META

scanning module

1-4 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Warning and Information Labels Carl Zeiss

Fig. 1-2 Warning and information labels on the Axio Imager.Z1 microscope with LSM 510 META

scanning module

03/06 B 45-0021 e 1-5

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Warning and Information Labels LSM 510 META DuoScan

40

50

30

60

20

70

10

80

100

90

Fig. 1-3 Warning and information labels on the Axioskop 2 FS MOT microscope with the

LSM 510 META scanning module

1-6 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Warning and Information Labels Carl Zeiss

Fig. 1-4 Warning and information labels on LSM DuoScan (systems LSM 510 DuoScan or

LSM 510 META DuoScan only)

Fig. 1-5 Warning and information labels on the system electronic rack

03/06 B 45-0021 e 1-7

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Warning and Information Labels LSM 510 META DuoScan

Fig. 1-6 Warning and information labels on laser components (page 1)

1-8 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Warning and Information Labels Carl Zeiss

Fig. 1-7 Warning and information labels on laser module LSM 5 LIVE

03/06 B 45-0021 e 1-9

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Warning and Information Labels LSM 510 META DuoScan

COHERE

n

T

E

n

TERPRISE

PEAK

SEARCH

E

n

TERPRISE

COHERE

n

E4

J37

E5

E4

J6

T

Fig. 1-8 Warning and information labels on laser components (page 2)

maximum

water
pressure
60 p.s.I.

OUT

water

1-10 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY LSM 510 META Regulations Carl Zeiss

1.3 Regulations

Extensive knowledge of the hardware/the system is indispensable for safe operation of the LSM 510 and
LSM 510 META.

Read these operating instructions and all device publications belonging to the system
conscientiously before operating the LSM 510 or LSM 510 META! You can obtain additional
information on the hardware configuration delivered and on optional system extensions from
the manufacturer or via the service hotline.

⇒ The LSM 510 and LSM 510 META have been designed, built and tested in conformity with the

following regulations and guidelines:

DIN EN 61010-1 (IEC 601010-1) "Safety requirements for electrical equipment for measurement,
control and laboratory use"

DIN EN 60825-1 (IEC publication 60825-1) "Safety of laser equipment", taking relevant CSA and UL
specifications into account

DIN EN 61326: “Electrical equipment for control technology and laboratory use – EMC­requirements”

Low voltage directive: 73/23/EWG
EMC directive: 89/336/EWG

⇒ The company works according to a certified Environment Management System according to

ISO 14001.

The Product was developed, tested and produced in accordance with the valid regulations and

guidelines for environmental law of the European Union.

The product and its accessories have been classified as instrument category 9 (laboratory equipment
or comparable standard). The product and its accessories agree with the EU-regulations 2002/95/EG
(RoHS) and 2002/96/EG (WEEE) , if applicable for the product.

Carl Zeiss has installed a process for taking back and recycling the instruments within the member
states of the European Union, which takes care of the appropriate utilization according to the said
EU guidelines.

For details on the disposal and recycling please refer to your relevant Carl Zeiss sales or service
organization.

The product must not be disposed in the household waste or through the municipal disposal
organizations. In case of resale the seller is obliged to inform the buyer, that the product has to be
disposed according to the said regulations.

03/06 B 45-0021 e 1-11

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Notes on Setting up the Microscope System LSM 510 META DuoScan

1.4 Notes on Setting up the Microscope System

Installation and commissioning of the LSM 510 and LSM 510 META system must be performed
by authorized Carl Zeiss service staff. The system should not be used prior to instruction by a
Carl Zeiss representative.

The LSM 510 or LSM 510 META laser scanning microscope is delivered in several crates.

The LSM 510 and LSM 510 META must be set up so as to ensure that the minimum clearance
between the wall and the rear of the system is no less than 0.5 m. This clearance is needed for
adjustment and maintenance operations.

Do not set up the unit in the proximity of heat sources such as radiators or direct sunlight. To avoid heat
build-ups, the ventilation slots on the microscope system must not be covered up.

The system must not be set up in areas with potential danger by explosives.

The unit must be connected to a properly installed socket outlet with earthing contact by means of the
mains cables supplied. Continuity of PE connection must not be affected by the use of extension leads.

The system contains components with dangerous voltage. The system must not be opened by
anybody else than authorized Carl Zeiss Service staff. Before opening the main plug has to be
disconnected.

Before connecting the mains cables, please check whether your mains voltage corresponds to
the voltage specified on the rating plate of the laser module.

For reasons of laser safety, all ports must either be equipped with the corresponding device
(scan head, camera, HBO lamp etc.) or covered with the counterpart of the laser safety kit
provided.

Maintenance, repair, modification, removal or exchange of components, or other interference
with the equipment beyond the operations described in this manual may only be carried out by
the manufacturer Carl Zeiss or by persons expressly authorized by Carl Zeiss to do so.
This applies especially to the microscope system, the laser scanning module, lasers, the PC
system, the power supply units, cable connections and other system components.

Please note that the LSM 510 and LSM 510 META are high-precision opto-electronic
instruments. Inexpert handling may easily impair their function or even damage them.

The openings for ventilation must not be covered.

There are hot surfaces on the HBO and HAL lamp.

When sliding the compact Lasermodule V in and out of the System electronic rack take care not
to catch your fingers.

1-12 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Notes on Setting up the Microscope System Carl Zeiss

After installation or conversion of the LSM system, authorized specialized staff must carefully check that it
is in a proper condition and, particularly, that covers protecting against laser radiation are provided.

Tube openings or other unused mounts should always be protected against dust and moisture with the
corresponding device components or with termination covers/blind plugs.

By establishing a corresponding workplace environment, please ensure that the formation of electrostatic
charges of electronic components is avoided.

To avoid vibrations during operation, the LSM 510 and LSM 510 META should only be operated in
conjunction with the system table (vibration damping).

03/06 B 45-0021 e 1-13

NOTES ON DEVICE SAFETY LSM 510 DuoScan Carl Zeiss Power Requirements LSM 510 META DuoScan

1.5 Power Requirements

The LSM 510 and LSM 510 META comes with a mains power supply cord and plug,
either CEE red (3/N/PE 400/230V/16A), or NEMA L 14-30P (2/N/Ground 120/240V/30A), and
with the matching mains socket outlet.
A ground wire (AWG10 green/yellow) is supplied because it is necessary to ground the system.
The connecting part on both ends of the cable is a cable eye with 8 mm inner diameter.
A suitable grounding point must be installed in the room.
For systems (220 ... 240 V AC) equipped with X-Cite 120 the mains socket outlet must be
equipped with a fuse having minimum tripping characteristic C according to IEC/EN 60898.

Line voltage 220 ... 240 V AC (±10 %) 100 ... 125 V AC (±10 %)

Line frequency 50...60 Hz 50...60 Hz

LSM incl. VIS laser

− Max. current

− Power

− Power consumption

Argon UV laser

− Line Voltage

− Max. current

− Power consumption

Class of protection I I

Type of protection IP 20 IP 20

Overvoltage category II II

3 phases at 16 A

Phase 1 = 1.9 kVA max.

Phase 2 = 1.5 kVA max.

Phase 3 = 2.6 kVA max.

5000 VA max. 5000 VA max.

208...240 V AC

(±10 %) 50 / 60 Hz

1 phase at 63 A

Note: For Line Voltage 220 V the
connector and power plug are rated
for 63 Amps, However wiring and
fuse should be rated for 32 Amps.

7000 VA max. 7000 VA max.

2 phases at 25 A

Phase 1 = 3.2 kVA max.

Phase 2 = 2.8 kVA max.

208...240 VAC

(±10 %) 50 / 60 Hz

1 phase at:

208 V: 34 Amps

230 V: 31 Amps

240 V: 29 Amps

Pollution degree 2 2

1-14 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Notes on Handling the Laser Components Carl Zeiss

1.6 Notes on Handling the Laser Components

The LSM 510 and LSM 510 META are laser hazard class 3B instruments. If equipped with a Ti:Sa
Laser, the LSM 510 and LSM 510 META are devices that belong to laser hazard class 4.
This moderate and high-risk class embrace medium-power and high power lasers. You must
take care not to expose yourself to the radiation of such lasers. In particular, never look into the
laser beam! Only personnel which has been instructed on laser safety is allowed to operate the
system.

The following laser types are currently intended for use in the LSM 510 and LSM 510 META. The use of
any other lasers as the ones listed below is not authorized.

Laser Class Power

1 Ar 351/364 nm (UV) 4* 80 mW

2 Diode laser 405 nm 3B 30 mW

3 Ar/ML 458/477/488/514 nm 3B 30 mW

4 HeNe 543 nm 3B 1 mW

5 DPSS 561 nm 3B 10 mW

6 HeNe 594 nm 3B 2 mW

7 HeNe 633 nm 3B 5 mW

8 Titanium:Sapphire Laser Mai Tai (Spectra Physics)
710-990 nm (depending on the model)

9 Titanium:Sapphire Laser Chameleon (Coherent)
710-980 (depending on the model)

4 2 W

4 2 W

10 Diode laser 405 nm 3B 50 mW

11 OPSS laser 488 nm 3B 100 mW

12 DPSS laser 532 nm 3B 75 mW

* Laser type class 4, if mounted on laser module with fiber output class 3B.

Please note that for the maintenance of the UV Laser it is recommended to run the laser at
maximum power once a day if the laser is not used frequently or only at low power levels. This
enables the Autofill process which keeps up the correct tube gas pressure. This operation
prolongs the life time of the tube and prevents complete tube failure if the laser is not used
for a prolonged period of time. For details please refer to the Operator’s Manual of the UV
laser.

Please contact Carl Zeiss if you intend to use a different laser other than the ones above.

If used properly, the LSM 510 and LSM 510 META will not pose any laser radiation risks for operating
staff. Nevertheless, you should observe the following warnings:

03/06 B 45-0021 e 1-15

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Notes on Handling the Laser Components LSM 510 META DuoScan

• If necessary - insofar as specified by law - inform the laser protection officer before
commissioning the laser.

• The laser modules are equipped with a key-interlock.

• Always store keys for laser key switches and, if applicable, keys for further laser power
supply units, where they are inaccessible to persons not authorized to operate the laser.

• A red LED on the front of the scan head lights up when one or all of the lasers are switched
on.

• Do not place any reflecting objects into the beam path.

• Never open any covers or panels.

• Never look into the laser beam, not even to simply view the specimen, whether with the aid

of optical instruments or without. Otherwise you risk going blind!

• Do not leave any empty objective positions of the nosepiece uncovered.

• If a class 4 laser is attached to the system, already stray light can impose danger to the

operator.

• With class 4 lasers take special care of fire protection requirements. Do not use or store
flammable or explosive solids, fluids or gases in the vicinity of the system.

• Class 4 lasers can inflame also flammable materials like cloth or paper. Do not put such
materials into the beam path.

• Do not reach into the process beam inside the sample area whenever the Class 4 laser is
active!

Suitable protective measures must be taken if gases, dust or vapors hazardous to health,
secondary radiation or explosive objects should arise on the specimen as a result of laser
radiation.

For NLO systems equipped with a specific push and click filter for NDD imaging be aware that
the NDD reflector cube in the reflector turret leads to a strong back reflection of HBO light into
the specimen plane and the eyepiece. When observing the specimen through the ocular lens
the use of the NDD reflector cube should be avoided. The light flash is not harmful but
unpleasant. The reflex of closing the eyelid is sufficiently protective. To completely avoid this
situation an additional filter (#1261-345) can be mounted into the NDD reflector cube which
prevents the back reflection of the HBO light in the ocular plane.

1-16 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY LSM 510 META Physical Dimensions Carl Zeiss

1.7 Physical Dimensions

Length (cm) Width (cm) Height (cm) Weight (kg)

Large system table 150 80 78 100

Small system table 65 80 78 60

Passively damped anti-vibration table 130 100 75 137

Active anti-vibration table (NLO)
for Mai Tai Laser or Chameleon

Active anti-vibration table (NLO)
for Verdi Mira or Millenia Tsunami
Laser

Scanning Module LSM 510 25 20 25 15

Scanning Module LSM 510 META 28 27 30.5 13

Scanning Module LSM DuoScan 40 15 13 8

Microscope 50 35 50 20

Laser Module RGB 110 70 28 95

Laser Module, UV 140 20 20 60

Laser Module V (405 nm) 66 52 22 30

Plug-in unit external laser 66 52 22 9

System Electronic Rack 110 70 58 90

Power supply for Ar (UV) 50 50 30 30

Cooling unit for Ar (UV) 80 45 50 30

Laser Module LIVE 66 52 22 58

120 140 75 200

180 140 75 400

1.8 Environmental Requirements

1. Operation, specified performance T = 22 °C ± 3 °C without interruption (24 h a day
independently whether system is operated or
switched-off)

2. Operation, reduced performance T = 10 °C to 35 °C, any conditions different from 1.
and 5.

3. Storage, less than 16 h T = -40 °C to 55 °C

4. Storage, less than 6 h T = -55 °C to 70 °C

5. Temperature gradient ± 0.5 °C/h

6. Warm up time 1 h, for high-precision and/or long-term measure­ments ≥ 3 h

7. Relative humidity < 65 % at 30 °C

8. Operation altitude max. 2000 m

9. Loss of heat 4 kW

These requirements do not include the requirements for high precision measurements. Please
refer to the Operator’s Manual of the microscope for these requirements.

03/06 B 45-0021 e 1-17

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss Notes on Handling the Computer and Data Media LSM 510 META DuoScan

1.9 Notes on Handling the Computer and Data Media

The computer used as standard in your LSM system is an IBM-compatible high-end Pentium computer
with WINDOWS XP operating system.

Do make sure, though, that you receive your LSM system with the operating system installed,
with initialization and start files set up and with the LSM program also installed.

When working with the hard disk, it is important to know that the more data it contains, the
slower its operation will become. Therefore, data that you do not need permanently should be
stored on other external devices.

When handling diskettes and USB sticks, avoid data losses by protecting them against extreme
temperatures, moisture and magnetic fields. The data on a diskette is stored in the form of
magnetic signals. To some extent, monitors, telephones or even lamps generate magnetic fields
that might destroy this data. Also, never open the metal cover on diskette cases. A diskette’s
surface can also be destroyed by touching it.

When handling CDs, CD ROMs or DVDs, do not touch the data side of the disc (the side of the
disc with no label or printing).

Do not apply paper labels or write on any part of the disc, data side or label side. If dust or
fingerprints get on the disc, wipe it with a soft cloth from the center to the edge, but do not
use benzine, paint thinner, record cleaner, or static repellent. This can damage the disc.

Do not place the disc in any place where it is exposed to direct sunlight or high temperatures.
Backup your data on a regular basis.
Do not install any other software without talking to your Carl Zeiss representative.

Never turn your computer off before you have terminated the LSM program and run down the
WINDOWS XP operating system. Otherwise, the program and/or data files may get lost.

1-18 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META Notes on Care, Maintenance and Service Carl Zeiss

1.10 Notes on Care, Maintenance and Service

The manufacturer of the unit cannot be held liable for damage resulting from operating errors,
negligence or unauthorized tampering with the device system, particularly as the result of removal or
replacement of parts of the unit or as the result of the use of unsuitable accessories from other
manufacturers.
Any such action will render all warranty claims null and void and also laser safety is no longer warranted.

You are well advised to arrange a service agreement with your nearest Carl Zeiss representative to
guarantee perfect functioning of the microscope system in the long term.

Modifications and conversion work on the components of the system must only be carried out by the
manufacturer, by the service agency or by persons authorized and trained for this purpose by the
manufacturer.

Damaged units or parts may only be repaired or maintained by the responsible service agency.

During maintenance or repair carried out by the service personnel the customer is requested to stand
aside and wear a pair of laser safety goggles if needed.

Before opening the housing of the halogen lamp switch off all laser units.

Care operations that may be carried out by operating staff are limited to cleaning painted and glass
surfaces.

• Before cleaning the instrument make sure the main power supply is disconnected.

• Cleaning painted surfaces

To do this, use a clean cloth that has been moistened in a mixture of water and some detergent; do
not use any solvent, however. Dry with a lint-free cloth.

• Cleaning glass surfaces

Glass surfaces that have become soiled or which are marked with fingerprints may be rubbed with a
clean optical cleaning cloth.
If soiling is persistent, dip the optical cleaning cloth into a mixture of distilled water and a small
quantity of detergent.
To complete cleaning, lightly breathe on the glass surface and rub it dry with a clean cloth. Lint or dust
is best removed with a clean brush.

• Make sure that no cleaning liquid penetrates into the system.

• Dust filters in the ventilation entries of the system electronic rack have to be replaced every 6 month.

For replacement please contact your local service representative.

03/06 B 45-0021 e 1-19

NOTES ON DEVICE SAFETY LSM 510 DuoScan Carl Zeiss User Interface LSM 510 META DuoScan

1.11 User Interface

All user interface ports are equipped with a safety interlock system which warrants laser safety.
These interlock devices must not be manipulated. Other interfaces which are not described here
are service interfaces and are only to be operated by authorized Carl Zeiss service personnel.
The following devices can be mounted and dismounted by the user:

− Halogen and HBO lamp

− Transmission PMT

− Switching Mirror

− Scan head

1.11.1 Mounting and Dismounting Lamps, TPMT and Switching Mirror

The ports of the lamps, the switching mirror and the transmission PMT are equipped with hardware
interlock devices. At most ports (all ports of the Axioskop 2 FS

MOT and the Axio Imager.Z1 as well as the

HBO port of the Axiovert 200 M) the following interlock devices are present and have to be operated in
the following way:

Interlock with sensor ring and contact ring:

Fig. 1-9 Sensor ring mounted to the interface

ports on the microscope side

Fig. 1-10 Contact ring mounted to the lamp,

TPMT or switching mirror

The interlock is working when the sensors of the sensor ring (
contact ring (

Fig. 1-10/1). Whenever this is not the case, for example if the distance between the two

Fig. 1-9/1) are depressed by the pins on the

devices is too large, the laser will be blocked and the system cannot be used.

1-20 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META User Interface Carl Zeiss

In case the system is not operating following the removal or attachment of any device on a port
with safety interlock check again the connection of the Contact ring to the Sensor ring.

For dismounting the lamps, TPMT or switching
mirror slightly unscrew the contact ring first
(

Fig. 1-10/2 or Fig. 1-11/2) so it can be moved

away from the sensor ring (

Fig. 1-11/1). Then
unscrew the lamp, TPMT or switching mirror (main
screw
Fig. 1-11/3) which is in one of the recesses of the
sensor ring (

Fig. 1-9/2). Hold the device to be
dismounted with one hand while unscrewing to
keep it from dropping. The now empty port has to
be closed with the blind cap to restore the
functionality of the system.

Use the main screw of

the port to fix the cap. Make sure the pins of the
cap depress the sensors of the sensor ring.

Do not remove the sensor ring from
the microscope. This might result in
failure of laser safety and a non
operating system.

For mounting any lamp, TPMT or the switching
mirror back onto the microscope reverse the steps
for dismounting the device. Be careful not to bend
the pins on the contact ring when screwing the
device onto the microscope port.

For the Axiovert 200 M transmission port and the
two ports available on the motorized switching
mirror no sensor ring is present. Instead the
sensors are directly installed at the Axiovert 200 M
transmission port or the two ports of the
motorized switching mirror.

Fig. 1-11 HBO lamp mounted to

Axiovert 200 M with sensor
ring and contact ring for laser
safety

1.11.2 Mounting and Dismounting the

Scan Heads

The scan heads are connected to the microscope
via an integrated safety interlock. They can be

Fig. 1-12 Blind cap for closing any port

equipped with an interlock device

moved between two microscopes. Make sure the
system is shut off completely before starting the
following procedure:

Be aware that the scan head weights up to 15 kg.

03/06 B 45-0021 e 1-21

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss User Interface LSM 510 META DuoScan

Moving the scan heads between Axiovert 200 M, Axioskop 2FS

• LSM 510 META scan head: Remove the blue cover from the back of the scan head (

Fig. 1-14).

MOT and Axio Imager.Z1:

⇒

Fig. 1-13 and

Fig. 1-13 Rear view of META scan head

• Loosen the screw on the lower end of the scan head (

Fig. 1-14 Rear view of META scan head with

blue cover removed

Fig. 1-15/1 and Fig. 1-16/1)

Fig. 1-15 Position of the screw on the

LSM 510 META scan head

1-22 B 45-0021 e 03/06

Fig. 1-16 Position of the screw on the

LSM 510 scan head

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META User Interface Carl Zeiss

• Loosen the screws on the port to the microscope for Axiovert 200 M (

Axio Imager.Z1 and Axioskop 2 FS

MOT (Fig. 1-18/1).

Fig. 1-17 Port connection between LSM and

Axiovert 200 M

Fig. 1-18 Fastening screws of the scan

head at the front of the tube
on Axioskop 2 FS
Axio Imager.Z1

Fig. 1-17/1) or to the tube on

MOT and

• Slowly pull the scan head away from the microscope port or the tube. For mounting the scan head

onto a microscope, make sure the pins and the electronic connections of the safety interface match
closely. Fasten the screws on the front of the tube (
followed by fastening the screw on the back of the scan head (

• Use the three fastening screws (

Fig. 1-19/1) to attach or detach the LSM DuoScan to or from the

Fig. 1-18/1) or at the microscope port (Fig. 1-17/1)

Fig. 1-15/1 and Fig. 1-16/1).

microscope stand.

Fig. 1-19 Fastening screws and connections on LSM DuoScan

03/06 B 45-0021 e 1-23

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss User Interface LSM 510 META DuoScan

For NLO systems being coupled to a Chameleon or Verdi Mira Laser from Coherent using the beam
housing kit designed for LSM 510, the beam tube connecting the periscope and the scan head has to be
removed before the scan head can be dismounted. Perform the following steps for the removal of the
tube:

• Unfasten the screw that clamps the outer tube to the inner tube (

• Pull out the tube from the periscope cover by sliding the inner tube into the outer tube (

• Turn the outer tube counter clockwise to unscrew the tube (

Fig. 1-21/3).

Fig. 1-21/6).

Fig. 1-21/4) from the small black tube

which is fixed to the scan head.

• Now the tube can be removed and you can proceed as described above.

• For rearranging the beam housing, first screw the tube to the scan head (

Fig. 1-21/4). It should not be
fastened completely to allow the inner tube to fit the side pin into the recess of the ring on the
periscope (

• Open the shutter in the periscope using an Allen key (

Fig. 1-21/1) and the recess of the outer tube (Fig. 1-21/2).

Fig. 1-20/1) and slide in the tube completely,

this will hold the shutter open.

Fig. 1-20 Shutter opening on the periscope.

Also applies for the large periscope
for upright microscopes.

Fig. 1-21 Tube connection from the periscope

housing (7) to the scan head (5).
Applies for upright and inverted
microscopes.

1-24 B 45-0021 e 03/06

LSM 510 NOTES ON DEVICE SAFETY
LSM 510 META User Interface Carl Zeiss

To ensure functioning of the system and laser safety the following connections have to be changed:

1. The connection of the microscope to the safety interface of the system is located either on the
additional Safety-Box (Axioskop 2 FS
200 M or Axio Imager.Z1;

Fig. 1-23/1 and Fig. 1-24/1). This connection has to be unplugged from

MOT, Fig. 1-22/1) or on the rear side of the microscope (Axiovert

the microscope which is not in use and plugged into the microscope to be used following the
exchange of the scan head.

Fig. 1-22 Safety-Box of Axioskop 2 FS MOT

with main connection to safety
interface (1)

1

Fig. 1-24 Connection of Axiovert 200 M to

safety interface (1)

Fig. 1-23 Connection of Axio Imager.Z1

to safety interface (1) and
electronics (2)

Fig. 1-25 Four CAN connections (1) are

available on the rear side of the
electronics module. The microscope
in use has to be connected to one
of them

03/06 B 45-0021 e 1-25

NOTES ON DEVICE SAFETY LSM 510 DuoScan
Carl Zeiss User Interface LSM 510 META DuoScan

2. The plug for the main connection of the
microscope to the electronics is situated on
the rear side of the electronics rack. It is either
of the four CAN connections shown in

Fig.
1-25. Only ONE microscope can be connected
to the electronics at a time. The connection
has to be plugged in and unplugged at the
electronics rack. The corresponding
connections on the microscopes are shown in
Fig. 1-23/2,
Fig. 1-26/1 and Fig. 1-27/1. Remove the
connection of the microscope no longer in use
at the electronics rack and plug in the
connection of the microscope which should be
used instead.

Fig. 1-26 CAN connections on the

Axiovert 200 M (1). One of
the plugs is occupied with the
connection to the electronics

Fig. 1-27 The Axioskop 2 FS MOT is connected

to the electronics via the Interface
Control. The CAN connection is
fixed (1) to the control box

3. After the connections of the non used
microscope are disconnected and the ones of
the used microscope are connected, the
system can be switched on again. Before
initializing the system with the LSM Software
make sure to use the right database according
to the microscope in use. It can be chosen via
the icon Stand Select.

1-26 B 45-0021 e 03/06