Olympus CHA, CHB User manual

OLYMPUS STUDENT MICROSCOPES

IINSTRUCTION MANUAL I

MODELS

CBA

&

CBB

Scanned

for

personal

by

J.

G.

McHone.8Nov

use

only.

not

for

09

sale

This instruction manual
CHA

and CHB.Itis

yourself

precision instrument.

Observe

•

fully

the

Operation

1.

Always

any

impact.

2.

Avoid

following points carefully.

exposureofthe microscopetodirect

3. Only use
knobs. Do

simultaneously,asthis

recommended

with

your

handle the microscope

the

tension

not

has

been prepared

that

microscope,inordertoobtain

you

for

read

IMPORTANT

with

the careitdeserves. and avoid

twist

adjustment

the

will

ring for altering

two

coarse

cause

damage.

adjustment

the

Olympus

the

manual

sunlight,

Student

carefullyinordertofamiliarize

optimum

dust

and

the

tensionofthe

knobsinthe

Microscopes Models

performance

abrupt

vibration.

coarse

opposite

from

this

motions

or

adjustment

directions

4.

Ascertain
conform

5.

Disconnect the line cord

• Maintenance

1.

Lenses

wiped
prints deposited
xylene, alcoholorether.

2.

Do
especially, should

3.

Never disassemble the microscope

4. The microscope should

possible,itshould be covered

that

the voltage selector switch on the

with

the

local mains voltage.

must alwaysbekept clean. Fine

offbymeansofan

on

not

use

organic solutionstowipe

be

from

the AC power

air

blowerora clean brush.

the lens surfaces

cleaned

with

a neutral detergent.

for

be

stored in its container immediately

with

the

outlet

dust

with

gauze moistened

the surfacesofvarious components. Plastic parts,

repair.

vinyl

dust

base

plateofthe Model

before fuse replacement .

on lens surfaces shouldbeblown

Carefully

cover provided.

wipe

with

after

CHAisset

off

oilorfinger-

a small

amount

use.Ifthisisnot

to

or

of

Scanned

for

personal

byJ.G.

use

CONTENTS

McHone.

only.

not

8

Nov

for

09

sale

STANDARD

I.

II.

VARIDUS

ASSEMBLY 4

III.

IV.

IDENTIFICATION

OPERATION .

V.

A.

AdjustmentofMinimum Line Voltage

B.

Placing a Specimen Slideonthe

Interpupillary Distance and Diopter

C.

D.

Tension

E.

Automatic

F.

Aperture

G.

Immersion Objectives

VI.

OPTICAL

EQUIPMENT 2

CDMPONENTS OF

AND

AdjustmentofCoarse

Pre·focusing Lever

Iris Diaphragm 9

DATA.

. .

THE

STUDENT

FUNCTION OF

Stage

Adjustment

MICROSCOPES MODELS

VARIOUS

Adjustments

CDMPONENTS

I<nabs

CHA

& CHB 3

5

7

8

10

VII.

TROUBLESHOOTING.

@ How

to

put

The blocks shouldbeput
wooden storage

1.

Insert

the

oneoflhe

in the drawing.

2.

Turn

the screwtogel

tightenitwith
a screwdriver.

3. Put the

other

microscope fixing blocks (provided

at the

bottomofthe

case

in the

following

screw

with

the

flat washer

two

holes (8

the

block in the above order.

mm4>1asshown

into

spanner provided

order:

into

the block and

or

withanoptional

Microscope

fixing

block

wooden

storage case)

<3

--jl-Bmm¢

Flat washer

.____---=.=c.

------

I

Wooden

storage case

"".w

10

I. STANDARD EQUIPMENT

Microscope

stage

Microscope stand

stage and in-base illuminator

Binocular

Mechanical

Abbe

stand

with quadruple revolving nosepiece, plain

and in-base illuminator

with

quadruple revolving nosepiece, plain

observation

stage

condenser

tube, inclined

with coaxial right-hand

Ach_

Ach_

(6V

(20W

4X

lOX

Objectives

Ach. S40X. spring-loaded 1

Ach. SlOOX,

Eyepieces

BiWF

Halogen light source CH-LSH

Model

lOW halogen)

tungsten)

45°

oil.

lOX

CHA-213-W

low

drive controls CH-MVR

CHA-F-W

CHB-F-W

BH-B

145-W

CH-CD

1 0

0

1 1

1 1

1 1

1 1

1 1

spring-loaded 1 1

2

lOW

CH-LSHB

HALCH

1 0

2 0

Lamp socket

Halogen bulbs

6V

CHB-213-W

1

1

2

Tungsten bulbs

fuses

O.5A

for

Spare

Eyepiece

Immersion oil, bottled 1 1

Vinyl

caps

dust cover 1 1

lOO-110-120V (or O.3A

for

220-240V) 2 2

20WCHB

0 3

2 2

• Optional Accessories:

Monocular tube. inclined

Mechanical stage

with

Tungsten bulb.6VlOW

Wooden storage case

45°

coaxial left-hand drive controls

for

CHA

CH-M045
CH-MVL
CH-6V lOW-

CHA-BIWSC

TP

2

II.

VARIOUS COMPONENTS OF THE STUDENT MICROSCOPES MODELS
CHA&CHB

These models

of

variety
This

Eyepiece

Revolving nosepiece

Objective

Plain stage

combinations, standardoroptional,isavailable accordingtoyour

is

a pictureofthe Model CHA.

are

composedofvarious components and interchangeable accessories. A

requirements.

Observation

Mechanical stage

tube

Stand

Base

Condenser

~

a

3

III.

ASSEMBLY

The

the assembly

picture

below

illustrates the sequential procedureofassembly.

orderofvarious components.

The

numbers indicate

* Take care

Securely

stage

to

the

clamping screws and tighten

them

with a coin.

\

@ Mechanical stage

Clamping screws

® Condenser

® Filter

Aligning the
condenser

insert

mount

with

at

assemblytokeep all

attach

the

mechanical II\.

the plain stage

glass

® Eyepiece cap

~'"

with

® Objective

11

---------~g~~g~O~L-®~9~connect

~

1

~

t

mount

the

from

condenser clamping screw.

~

positioning

mount

and condenser,

condenser

below and clamp

dots

into

surfaces clean and avoid scratching the lens surfaces.

(J)

Eyepiece

""">

""-

~

~

Observation

Observation tube

tube

! clamping screw

Stand

th~outlet.

on

the

A Line

~~~

Pull

down

the lamp house cover.

the locking

cord

the plug

knob

to

of

IFor CHB I

I

ForCHA

IFor

CHA

Tungsten bulb

20WCHB

®::J

Halogen Lamp socket

bulb CH-LSHB

I

0--

I

Tungsten

6V10W

Q:::J

Q

bulb

CD

*

* In

* Before use,

4

After

opening the lamp house

cover

at

the microscope base,

press the tungsten bulb socket

against the

rotate

For

useofa halogen

its contact pins into the socket.

caseofthe tungsten

silver reflecting surface must

positioned pointingtothe

plate.

or stains on the resPective bulbs.

lamp

clockwise.

wipe

mount,

bulb,

off

fingerprints

insert

bulb,

and

its
be

base

IV.

IDENTIFICATION

AND

FUNCTION OF

VARIOUS

COMPONENTS

Mechanical tube length
adjustment ring

Rotate the

interpupillary

tained
diopter

ringtomatch

distance

from

the

adjustment.

scale. and make

Specimen holder

Low

drive coaxial stage

trois

setting

your

ob-

con~

Interpupillary

distance scale

Observation tube clamping

screw

Loosen the

to

desired.

Graduatedinincrementsof2.5J.L

rotate

clamping

the observation

screw

Tension adjustment ring

Coarse adjustment

Fine

adjustment

slightly

tube

knob

knob

as

•

....

....

<O'!'!

..............

Rheostat trimmer screw

After switching on,ifnecessary.

rotate

this

screw

the

bulbisdimly

sliding

control

voltage position.

Fuse holder

withacoin

switchatminimum

lit,

with

until

the

Main switch

For

continuously

intensitY.

Sliding

control

variable

Grounding

Line voltage selector switch

(For

CHA)

Set

form

with

the

the

local mains voltage.

lever

light

terminal

switchtocon-

5

Automatic
pre-focusing lever

Condenser height adjustment

knob

The

condenser is generally used

at

top
objectives
however,itis
lower
eliminate
tion.

position.

the condenser

For

lOX

and

lower

recommended

uneven field

use

power,

properly

illumina-

with

Aperture iris

diaphragm lever

Filter

mount

Slip-in

type

.

..........

to
to

~\~._._~I_~_-,---:::-F~il~te~r-:.mc:07u~n~t

Accepts

45mm

diam.

filters.

Lamp

mount

§iJx~o

6

Lamp house clamping knob

The

lamp

house

cover
openedbypulling
or

closedbypushingitup

snapsinplace.
Before pushing, ascertain

knobispositionedasshown
the

picture

circle.

right,

down

marked

can be

the

until

that

knob;

it

the

in

with

V. OPERATION

• SummaryofPutting

1.

Match the line voltage selector switchtolocal mains voltage
page

5,)

2. Switch on

3.

Adjust the

4.

Place

a specimen slide on the

5.

Loosen the

6.

Coarse

focus

7.

Make interpupillary

8.

Swing in the desired objective.

9.

Adjust light intensity.

the Microscope in

the

light source.

trimmer

automatic

withalow

screw.

(See

page

stage.

pre-focusing lever.

power objective.

and

diopter

adjustments.

Operation

7.1

(See

page

7.)

ISee

page

(for

8.)

10. Fine focus.

11. Lock the automatic pre-focusing lever.

12. Adjust the aperture iris diaphragm. ISee

A. AdjustmentofMinimum

The

minimum

trimmer
frequency.

built·in

The

voltage required

screw

at

rheostat incorporates a

Line Voltage (CHA)

the microscope

for

the ligh t source can be adjusted

base

thyristor

(See

page

8.1

page

9.1

plate

in accordance

in its semi-conductor

with

with

the

circuit

advantages:

(a)

Extremely

(bl Flickeringofthe bulb filamentiseliminated and the light intensityisstabilized.
Icl

Increased life expectancyofthe bulb.

For

adjustmentofthe

that

the voltage selector switchissettoconform
the local mains voltage, and the sliding
is

positioned closest

activate the main switch

the secondary
rotate the rheostat
coin,

until

control

intensity. (Fig. 1)

8.

Placing a Specimen Slide on the Stage
NOTE:

fine

adjustmentoflight

minimum

to

you

CD.

VOltageiscorrect.Ifitisnot

trimmer

the

bulbisdimly

lever

forwardinordertoobtain

1) Cover

2) Specimen slide:

glass:

intensity

line

voltage, ascertain

control

(low

voltage). and then

If

the

bulbisdimly

screw @ gradually

lit;

then push the sliding

canbeeasily achieved.

with

lever

CV

lit,

lit

at all,

with

a

optimum

light

Fig. 1

Olympus objectives
use

with

cover

Itisrecommendedtouse
thickness.

However,

for

withanengraving

glassesof0.17mm

specimen slidesofO.8mmto1.5mm

use

with

the immersion

"0.17"

thickness (No. 1%).

darkfield

BH-DCW (optionally availablel. a specimen slide between

O.8mmto1.2mm thicknessispreferable.

CHAI.

line

for

are corrected

(See

the rheostat

voltage and

the

following

condenser

for

7

C.

Interpupillary

1)

Hold
left

tubes together,
ever
with
obtained (Fig.

21

Rotate the tube length adjustment ring ®

right eyepiece tube

(

distance setting which

@.

Distance and

the knurled dovetail slides Q)ofthe

eyepiece tubes

or

is

required,

both

while

eyes,

21.

Diopter

with

pull them apart laterally, which-

looking

until

perfect binocular vision

to

you

Adjustments (Binocular tube)

both

hands and push the

through

match

your

obtained

right

the

eyepieces

on

interpupillary

from

the scale

and

is

the

Fig.2

(

3)

Look at the image

your

right eye and focus on the specimen

coarse and

4)

Next,

length adjustment ring
adjustment knobs.

fine

looking

through

adjustment knobs.

at the image through the

@

the

to

focus on

right

eyepiece

with

left

eyepiece

the

specimen

with

the

with

without

your

left

eye

rotate

using the coarse and fine

the tube

D. Tension

A tension adjustment ring
right hand coarse adjustment knob.
tension
either heavyorlight
preference. However,
ment ring
droporthe fine adjustment knobstoslip.
The

*

Be
ment

E.

Automatic

This lever

between specimen and objective

,

coarse focusing. The leverislocked
has
travel
knobs, and automatically provides a
if
automatic
ing. (Fig. 4)

AdjustmentofCoarse

of

the coarse adjustmentisfreely adjustable

movement depending on operator
do

too

much, because this may

arrow

mark indicates increaseofthe tension.

careful

the stage

nottorotate the

knobs in the opposite directions simultaneously.

Pre·focusing Lever

CD

is

providedtoprevent possible contact

been accomplished. This prevents

of

the

stage

by meansofthe coarse adjustment

is

lowered and then raised again. The

pre-focusing lever does

Adjustment

CD

is

provided

not

loosen the tension adjust-

right

and

as

not

Knobs

With

this device the

cause

left

coarse adjust-

wellasto

after

coarse focus

further

limiting

restrict

nexttothe

thE~

fine

for

stage

simplify

upward

stop

focus-

to

Fig. 3

Fig.4

8

F. Aperture Iris Diaphragm

An

aperture iris diaphragmisprovidedonthe condenser,
the openingofwhich
numerical aperture
achieve
focus, image contrast and resolution.
However, since microscopic specimens generally are
in contrast, their image lacks contrastjfthe objective
used
often

optimum

with

its

preferabletostop

canbeadjustedtomatch

of

the objective in use, in order

objective performance,asdepth

full

numerical aperture. Therefore,itis

down

the aperture diaphragm

with

the

of

low

to

is

70-80%

slightly more than indicated by the objective N.A. An

aperture setting at 70%to80%ofthe objective N.A.isrecommended.Ifnecessary, remove
the eyepiece and, looking at the

exit

pupilofthe objective, adjust

the

openingofthe

diaphragm.

G.

Immersion Objectives

To

utilize

the

full
homogeneous
sion oil.

*

Care

shouldbetakentoprevent oil bubbles

specimen

numerical apertureofan

immersion). the objective, specimen and condenser are immersedinan immer-

sl

ideorobjective.

immersion objective

from

forming

in the oil

(with

engraving

film

between condenser,

"HI"

for

After

use,

carefully wipe

with

gauze moistened

Never

leave

oil on lens surfaces after

performance

for

immersion objectives.

oil

off

with

xylene.

of

the lens systems.Itis

the immersion oil deposited on the lens surfaces

useasoil

recommendedtouse

remnants

will

seriously

Olympus immersion

impair

the

9

VI. OPTICAL DATA

Objective

.,

Eyepiece loaded loaded

WFlOX

(Field

number

18)

..

Immersion objective.

*·The

• Working distance: The distance

• Numerical aperture:

• Resolving power:

• Focal

resolving powerisobtainedifthe objectiveisused

diaphragm.

depth:

Type

Magnification

NA

W.D.(mm)

Focal length

Resolving power**{J-L)

Remarks

Total

Focal depth

(mml

magnification

(Il)

4X

0.10

19.87 5.40 0.39 0.11

29.20 15.98 4.31

3.4 1.3 0.52 0.26

40X

172.5 27.60 3.03

Fieldofview (mm) 4.5

from

the specimenorcover glasstothe nearest

of

the objective.

The

N.A.

represents a performance
comparedtothe
of

light

which

the

squareofthe

The

resolving

two

points.

The

distance between

image

formedbyan

relativ(~

the objective receives

performance number.

powerofa lens is measuredbyits

the

optical

Achromat

lOX

S40X

0.25 0.65 1.30

Spring- Spring-

100X 400X 1000X

1.8

with

apertureofa camera lens. The

from

upper

and

lower

system. As

0.45 0.18

the

fully

opened aperture

number

the object- increases

you

which

abilitytoseparate

limitsofsharpness in the

stop

down

the aperture

Sl00X'

1.81

0.66

point

could

quantity

be

with

iris diaphragm, the focal depth becomes deeper. The larger the N.A.

of

the objective the shallower the focal depth.

• Field

•

number:

Field-of-viewdiameter:

A number
field diaphragm

The

that

actual sizeofthe

represents the

thatisformedbythe lensinfrontofit.

fieldofview

diameterinmmofthe imageofthe

in mm.

VII.

TROUBLESHOOTING

Troubles

1. Optical System

a)

Fieldofviewiscut
illuminated

irregularly.

off,

or

Nosepiece

Condenser is
on the ring

Causes

did

not

not

mount.

change

correctly

10

properly.

mounted

Remedies

Slightly
it

Re-insert the condenser all the way.

clicks

rotate

into

the

position.

nosepiece

until

Troubles

Causes

Remedies

Dust

bl

dirtisvisible in Dust or

or

the fieldofview. the light

Dustoncondenser top

Dirty

Dust

Excessive

cl

Resolution problems:

dl

·

·

·

image contrast.

I

mageisnot sharp.

Insufficient contrast.

Image

details lack

finition.

Condenser

Aperture iris diaphragm

down excessively.

Objective

ed

Dirtonobjective

de-

Immersion objective

out immersion oil.

Bubbles in the immersion oil.

Olympus immersion oilisnot

Dirty

Dust

top

dirtonthe

exitonthe

specimen.

on

eyepiece.

is

lowered excessively. Raise the condenser.

is

not correctly position- Slightly

in the light path.

front

specimen.

eyepieces and condenser

on

lens.

glass

base.

lens.

lens.

is

surface

is

used

at

Remove dust or dirt.

stopped Open the diaphragm.

rotate the nosepiece until

it

clicks into position.

Clean the objective.

with-

Apply

immersion oil.

Remove bubbles.

used.

Use

Olympus immersion oil.

Clean.

e)

Field of view

of

focus.

out

f)

When objectives are
ed

they are not parfocal.

g)

Light

intensity does

increase although the volt­ageisraised.

2.

Electric System

a)

Illuminator

(or

too

dark). matched with the mains voltage voltage.

bl

Output

luminator cannot
ulated.

voltage

is

partially Objectiveisnot correctly position-

is

too

for

ed

Specimen

ed

on

chang~

Mechanical tube

rectly adjusted.

Condenserislowered excessively.

not

Rheostat trimmer screw

rectlyadjusted.

bright Voltage

(for

Mains voltage

low).

Rheostat trimmer screw

rectly adjusted.

the

be

Voltage

11-

reg-

matched with the mains voltage voltage.

(for

in the Iight path.

is

not correctly position- Place the specimen on the

the

stage.

selector switch

CHA).

is

selector switch

CHAI.

length

too

Slightly

it

clicks into position.

secureitwith the specimen holder

stage

or

is

not cor-

is

not cor- Adjustitcorrectly.

is

high (or

is

not cor- Adjustitcorrectly.

is

Adjust

ment

ringsonthe observation tube.

Raise

the condenser.

not

Set the switch

too

Adjust

variable voltage transformer.

not

Set the switch to match the mains

rotate the nosepiece until

stage

clips.

with

the

tube

length adjust-

to match the mains

the mains voltage with

and

a

Mains voltageistoo

high).

low

(or too Adjust the

mains voltage with a

variable voltage transformer.

c)

Light flickers

tensity

Troubles

is

unstable.

Causes

and

the in· Mains voltageisunstable.

of

Filament

burn out.

Loose

the

electrical connection. Secure the connection.

bulbislikely

Use

a voltage stabilizer.

to

Replace the bulb.

Remedies

dl

Fuse

burns

e)

Bulb does

f)

Reduced bulb life.

out

too

often.

not

light. Bulb

Fuseisnot a standard fuse.

Voltage

matched with the mains voltage. voltage.

Loose

selector switch

is

burned out. Replace the bulb.

electrical connection. Secure the connection.

Voltage selector

matched with the mains \loltage. the mains voltage.

Bulb

is

not a standard one.

Bulb

was

over vol ted too long. Reduce

3. Focusing

a)

Coarse

tight.

b)

Stage drops and the speci- Tension adjustment ring
men

adjustment

goes

outoffocus.

is

Tension adjustment

too

ed

too much. slightly.

Useristrying to
sing

over the upper focusing limit
imposed by the
ing

lever.

loose.

raise

engaged

switch

ring

the

Use

a standard fuse.

is

not

Set the switch to match the mains

is

not

Set the selector switch to match

Use

a standard bulb.

bulb

voltage.

is

tighten- Loosen the tension adjustment ring

stage,

pas-

Unlock the pre-focusing lever.

pre-focus-

is

Tighten the ring slightly.

too

c)

Stage cannot

the upper limit.

dl

Stage

cannot be lowered

to the lower limit
working

Objective front

el

es

the specimen. upside down.

4.

Binocular Observation Tube

Incomplete binocular

al

sion.

range.

be

lens

raised

of

touch-

to

the

Specimen

vi-

rectlyadjusted.

matched.

vision.

Pre-focusing lever
lower than focusing position.

is

Substage

Interpupillary distance

Diopter adjustmentisincomplete. Complete the diopter adjustment.

Right and

User

loweredtomuch.

is

mounted on the

left

is

unaccustomed to binocular Prior to looking at the image of the

engaged

is

eyepieces

not cor-

is

are

in

stage

not

Unlock the pre·focusing lever.

Raise

the

substage.

Reverse the specimen.

Correct the interpupillary distance.

Use

a pairofmatched eyepieces.

try

specimen,

of

field
object before resuming microscopic
observation.

to look at the entire

view, or look at a far away