Metrohm 732IC User Manual

CH-9101 Herisau/Switzerland
Phone ++41 71 353 85 85
Fax ++41 71 353 89 01

CompuServe 100031,3703
Internet http://www.metrohm.com
E-Mail [e-mail]

732 IC Detector
733 IC Separation Center

5.732.0012 Program

IC Detector732

+0.023 µS/cm 15.2 min
Full Scale 5.00 µS/cm

OVERLOAD

THERMOSTAT

FULL

PARAM

SCALE

IC Separation Center733

MetrohmMetrohm

METHOD

PUMP R/S

CONFIG

7

8 9

CONFIG

EVENT

PROGRAM

ZERO OFF

4

5 6

PRINT

REPORT

PLOT

1

2 3

MARK

PROG

R/S

0

.

SELECT CLEAR

QUIT ENTERZERO

-

/+

MetrohmMetrohm

FILL INJECT

A B

STEP

FILL INJECT

8.732.1033 Instructions for Use

1.10.1998 / dö

Table of contents

Table of contents

1 Introduction............................................................................................1

1.1 Instrument description.............................................................................1

1.2 Parts and controls..................................................................................... 3

1.2.1 732 IC Detector .............................................................................3

1.2.2 733 IC Separation Center..............................................................5

1.3 Information on the Instructions for Use................................................8

1.3.1 Organization .................................................................................. 8

1.3.2 Notation and pictograms............................................................... 9

1.4 Safety notes .............................................................................................10

1.4.1 Electrical safety............................................................................10

1.4.2 General precautionary rules........................................................10

2 Installation ...........................................................................................11

2.1 Flow chart.................................................................................................11

2.2 Setting up the instruments....................................................................12

2.2.1 Packaging....................................................................................12

2.2.2 Check ..........................................................................................12

2.2.3 Location.......................................................................................12

2.2.4 Arrangement of the instruments..................................................12

2.3 Connection of 733 IC Separation Center.............................................13

2.3.1 733.0010/733.0X30 IC Separation Center................................... 13

2.3.2 733.0X20 IC Separation Center................................................... 14

2.3.3 Connection of syringe and suction tubing.................................. 16

2.3.4 Connection of the drain tube....................................................... 16

2.3.5 Connection of the 6.5324.000 Bottle rack (option) ..................... 16

2.4 Mains connection....................................................................................17

2.4.1 Setting the mains voltage............................................................ 17

2.4.2 Fuses........................................................................................... 18

2.4.3 Mains cable and mains connection ............................................18

2.4.4 On/off switching of the instruments ............................................18

2.5 Capillary connections.............................................................................19

2.5.1 Capillaries.................................................................................... 19

2.5.2 Steel connectors.......................................................................... 19

2.5.3 PEEK connectors ........................................................................20

2.6 Connection of 709 IC Pump...................................................................21

2.6.1 Electrical connection ................................................................... 21

2.6.2 Pulsation dampener .................................................................... 21

2.6.3 Filter unit PEEK............................................................................ 22

2.6.4 Filter unit Manufit ......................................................................... 23

2.6.5 Connection to injection valve with PEEK capillaries ...................24

2.6.6 Connection to injection valve with steel capillaries..................... 25

2.6.7 Passivation of the IC system.......................................................27

2.7 Precolumns..............................................................................................28

2.7.1 General information on precolumns............................................28

2.7.2 Precolumns with twin cartridge holder........................................28

2.7.3 Precolumns with cartridge head .................................................30

2.7.4 IC anion precolumn SUPERSEP ................................................. 31

732 IC Detector / 733 IC Separation Center

I

Table of contents

3 Operating tutorial ........................................................................45

2.8 Separating columns and suppressor module.................................... 32

2.8.1 General information on separating columns .............................. 32

2.8.2 Selection of the sample loop ...................................................... 32

2.8.3 General information on suppressor module............................... 33

2.8.4 One-channel system without suppressor module...................... 35

2.8.5 Two-channel system without suppressor module...................... 35

2.8.6 One-channel system with suppressor module........................... 37

2.8.7 Leak testing and conditioning..................................................... 40

2.9 Connection of external devices............................................................ 41

2.9.1 Connection of a recorder ............................................................ 41

2.9.2 Connection of «IC Metrodata for Win95».................................... 41

2.9.3 Connection of the 750 Autosampler ........................................... 41

2.9.4 Connection of the 766 IC Sample Processor ............................. 41

2.9.5 Connection of the 791 VA Detector ............................................ 41

2.9.6 Connection of a printer ............................................................... 42

2.9.7 Connection of a PC..................................................................... 44

2.9.8 Connection of devices to the remote interface........................... 44

3.1 Requirements ..........................................................................................45

3.2 Preparations............................................................................................. 46

3.3 Putting into operation............................................................................ 48

3.4 Calibration................................................................................................ 59

3.5 Sample determination............................................................................ 62

3.6 Storing as a method............................................................................... 64

4 Operation ...............................................................................................67

4.1 Operating sequences............................................................................. 67

4.1.1 General flow chart ....................................................................... 67

4.1.2 Flow chart for basic settings....................................................... 68

4.1.3 Flow chart for putting into operation ........................................... 69

4.1.4 Flow chart for injection ................................................................ 70

4.2 Fundamentals of the operation ............................................................ 71

4.2.1 Display......................................................................................... 71

4.2.2 Overview of key functions ........................................................... 72

4.2.3 Instrument dialog ........................................................................ 77

4.2.4 Data entry.................................................................................... 79

4.2.5 Text entry..................................................................................... 80

4.3 Displays in the standby mode.............................................................. 81

4.3.1 Measured value and current time ............................................... 81

4.3.2 Status messages ........................................................................ 82

4.4 Basic settings.......................................................................................... 84

4.4.1 Setup........................................................................................... 84

4.4.2 Configuration, <CONFIG> key.................................................. 88

4.5 Measurement parameters...................................................................... 97

4.5.1 <PARAM> key ........................................................................... 97

4.5.2 <FULL SCALE> key................................................................. 102

II

732 IC Detector / 733 IC Separation Center

Table of contents

4.6 Triggering of functions ........................................................................103

4.6.1 <FILL> keys .............................................................................103

4.6.2 <INJECT> keys........................................................................104

4.6.3 <ZERO> key ............................................................................ 105

4.6.4 <ZERO OFF> key ...................................................................106

4.6.5 <MARK> key............................................................................ 106

4.6.6 <PUMP R/S> key.....................................................................106

4.7 Programming .........................................................................................107

4.7.1 <PROGRAM> key.................................................................... 107

4.7.2 <PROG R/S> key..................................................................... 114

4.7.3 <EVENT> key........................................................................... 114

4.7.4 <METHOD> key ......................................................................117

4.8 Data output.............................................................................................119

4.8.1 <PRINT> key............................................................................ 119

4.8.2 <PLOT> key.............................................................................122

4.8.3 <REPORT> key........................................................................ 124

4.9 Examples of methods...........................................................................126

4.9.1 Cation determination with Metrosep Cation 1-2........................126

4.9.2 Anion determination with Metrosep Anion Dual 2.....................129

5 Notes – Maintenance – Faults ................................... 133

5.1 Practical notes on ion chromatography............................................133

5.1.1 Separating columns ..................................................................133

5.1.2 Pumps .......................................................................................134

5.1.3 Eluents.......................................................................................135

5.1.4 Suppressor module...................................................................136

5.1.5 Connections .............................................................................. 136

5.2 Maintenance and servicing..................................................................136

5.2.1 General information................................................................... 136

5.2.2 Passivation ................................................................................137

5.2.3 Recycling................................................................................... 137

5.2.4 Shutdown...................................................................................137

5.2.5 Changing separating columns..................................................138

5.2.6 Regeneration of the suppressor................................................140

5.2.7 Cleaning the suppressor........................................................... 141

5.2.8 Replacing the suppressor......................................................... 143

5.3 Faults and malfunctions ......................................................................145

5.3.1 Error messages .........................................................................145

5.3.2 Malfunctions and their rectification ...........................................148

5.4 Diagnostic tests.....................................................................................149

5.4.1 General...................................................................................... 149

5.4.2 Preparing instruments ............................................................... 150

5.4.3 Checking working memory (RAM) ............................................ 151

5.4.4 Check keypad ...........................................................................151

5.4.5 Check display............................................................................152

5.4.6 Check RS232 interface.............................................................. 153

5.4.7 Check remote interface.............................................................154

5.4.8 Internal hardware test................................................................ 155

5.4.9 Initialize data memory................................................................157

5.5 Validation / GLP.....................................................................................159

732 IC Detector / 733 IC Separation Center

III

Table of contents

6 Interfaces .......................................................................................... 161

6.1 RS232 interfaces................................................................................... 161

6.1.1 General rules for remote control ............................................... 161

6.1.2 Call-up of objects...................................................................... 162

6.1.3 Triggers .....................................................................................163

6.1.4 Status messages ...................................................................... 164

6.1.5 Error messages......................................................................... 165

6.1.6 Remote control commands ...................................................... 166

6.1.7 Data transmission protocol....................................................... 177

6.1.8 Handshake................................................................................ 178

6.1.9 Pin assignment.......................................................................... 181

6.1.10 RS232 error rectification............................................................ 182

6.2 Remote interfaces.................................................................................183

6.2.1 "Remote" interface..................................................................... 183

6.2.2 "733 IC Separation Center" interface......................................... 185

6.3 Analog output........................................................................................ 187

6.4 External power supply for 733 IC Separation Center......................187

6.5 Valve interfaces..................................................................................... 188

7 Appendix ............................................................................................. 189

7.1 Technical data ....................................................................................... 189

7.1.1 732 IC Detector ......................................................................... 189

7.1.2 733 IC Separation Center.......................................................... 192

7.2 Standard equipment............................................................................. 193

7.2.1 732 IC Detector ......................................................................... 193

7.2.2 733 IC Separation Center.......................................................... 194

7.3 Optional accessories............................................................................ 196

7.3.1 Accessories for 733 IC Separation Center................................ 196

7.3.2 Separating columns and precolumns....................................... 198

7.3.3 Additional devices and cables.................................................. 202

7.4 Warranty and conformity.....................................................................204

7.4.1 Warranty .................................................................................... 204

7.4.2 EU Declaration of conformity .................................................... 205

7.4.3 Certificate of conformity and system validation........................ 207

7.5 Index........................................................................................................ 209

IV

732 IC Detector / 733 IC Separation Center

Table of contents

List of figures

Fig. 1: Block diagram of the ion chromatography system ........................................... 2

Fig. 2: Front of the 732 IC Detector .............................................................................. 3

Fig. 3: Rear of the 732 IC Detector............................................................................... 4

Fig. 4: Front of the 733 IC Separation Center............................................................... 5

Fig. 5: Rear of the 733 IC Separation Center................................................................6

Fig. 6: Connection 732 – 2.733.0010/2.733.0X30 ...................................................... 13

Fig. 7: Connection 732 – 2.733.0X20 ......................................................................... 15

Fig. 8: Setting the mains voltage ................................................................................ 18

Fig. 9: Connectors for capillaries................................................................................ 20

Fig. 10: Connection of 709 IC Pump ............................................................................ 21

Fig. 11: 6.2821.100 Filter unit PEEK .............................................................................22

Fig. 12: 6.2821.000 Filter unit Manufit...........................................................................23

Fig. 13: Connection to injection valve with PEEK capillaries........................................25

Fig. 14: Connection to injection valve with steel capillaries .........................................26

Fig. 15: Installing precolumn cartridges ....................................................................... 29

Fig. 16: Interior of the 733.0010 IC Separation Center .................................................34

Fig. 17: Interior of the 733.0X30 IC Separation Center................................................. 36

Fig. 18: Connections at suppressor module................................................................38

Fig. 19: Ion chromatogram of the calibration ...............................................................61

Fig. 20: Ion chromatogram of the drinking water sample ............................................63

Fig. 21: Schematic representation of the instrument dialog......................................... 78

Fig. 22: Ion chromatogram for cation standard with Metrosep Cation 1-2 ................ 128

Fig. 23: Ion chromatogram for anion standard with Metrosep Anion Dual 2 ............. 131

Fig. 24: Assembling the suppressor........................................................................... 142

732 IC Detector / 733 IC Separation Center

V

Table of contents

List of numbered parts and controls

11 Display ................................................3

22 Main function keys ..............................3

33 Numeric keys ......................................3

44 Auxiliary function keys.........................3

55 Program status display ....................... 3

66 Auto-zero display ............................... 3

77 Thermostat display.............................. 3

88 Overload display .................................3

99 Mains switch ..................................4,18

1010 Serial number......................................4

1111 Analog output 0…1 V.......................... 4

1212 Analog output 0…10 mV.....................4

1313 Connection for detector block ............4

1414 Connection for

733 IC Separation Center....................4

1515 Connection for 709 IC Pump ..............4

1616 RS232 interface...................................4

1717 Remote interface.................................4

1818 Mains connection plug...................4,18

1919 Fuse holder ....................................4,18

2020 Door to interior ....................................5

2121 Connection for syringe........................ 5

2222 Feedthrough for aspirating tubing ......5

2323 „FILL“ for valve A................................. 5

2424 „INJECT“ for valve A ...........................5

2525 „FILL“ for valve B.................................5

2626 „INJECT“ for valve B ...........................5

2727 Connection/Feedthrough....................5

2828 Feedthrough........................................5

2929 Opening for detector cable B .............6

3030 Opening for outlet capillary B .............6

3131 Rear panel opening.............................6

3232 Opening for outlet capillary A..............6

3333 Opening for detector cable A..............6

3434 Knurled screw .....................................6

3535 Detachable rear panel.........................6

3636 Connection for 732 IC Detector A.......6

3737 Knurled screw .....................................6

3838 Terminal block for valve A................... 6

3939 Detachable rear panel.........................6

4040 Opening for inlet capillary A................6

4141 Rear panel opening.............................6

4242 Connection for drain tube ...................6

4343 Rear panel opening........................6,36

4444 Opening for inlet capillary B................ 6

4545 Connection for 732 IC Detector B....... 6

4646 Terminal block for valve B...................6

4747 Model plate .........................................6

4848 Connection for external supply...........6

4949 Ferrule ..........................................20,29

5050 Pressure screw.............................20,29

5151 Capillary ............................................20

5252 Compression fitting...........................20

5353 Connector with filter ..........................22

5454 Housing for filter unit......................... 22

5555 Connector .........................................22

5656 Inlet capillary ................................23,26

5757 Manufit pressure screw................ 23,29

5858 Counterpart end................................23

5959 PTFE gasket................................. 23,29

6060 4 Steel meshes ............................23,29

6161 Steel mesh holding end.................... 23

6262 Manufit housing ...........................23,29

6363 Outlet capillary .............................23,26

6464 Filter unit PEEK .................................25

6565 PEEK capillary.........................25,34,36

6666 Pulsation dampener................25,34,36

6767 Column connection

capillary..............................25,29,34,36

6868 Injection valve..........................25,34,36

6969 Filter unit Manufit............................... 26

7070 Coupling............................................ 26

7171 Steel capillary.................................... 26

7272 Outlet capillary ..................................29

7373 2 Steel meshes .................................29

7474 Precolumn cartridge..........................29

7575 Inlet capillary .....................................29

7676 IC separating column..............29,34,36

7777 Manufit pressure screw..................... 29

7878 Steel spacer ...................................... 29

7979 Steel connector for ferrule................. 29

8080 Manufit housing ................................29

8181 Detector block.............................. 34,36

8282 Inlet capillary for detector block...34,36

8383 Mounting rail ................................34,36

8484 Column holder .............................34,36

8585 Capillary for syringe .....................34,36

8686 Sample loop.................................34,36

8787 Inlet capillary for injector .............. 34,36

8888 PTFE aspirating tubing.................34,36

8989 Suppressor inlet capillary

for eluent ...................................... 36,38

9090 Suppressor inlet capillary

for H2SO4......................................36,38

9191 Suppressor outlet capillary

for H2SO4......................................36,38

9292 Suppressor outlet capillary

for H2O .........................................36,38

9393 Suppressor inlet capillary

for H2O .........................................36,38

9494 Suppressor outlet capillary

for eluent ...................................... 36,38

9595 Suppressor module ..........................36

9696 Coupling............................................ 36

9797 Screw nut ........................................ 142

9898 Connection piece............................ 142

9999 Suppressor rotor .............................142

100100 Suppressor holder .......................... 142

VI

732 IC Detector / 733 IC Separation Center

1 Introduction

1.1 Instrument description

The 732 IC Detector is a conductivity detector especially designed for
ion chromatography with an extensive operating range and high sensi­tivity for the recording of chromatograms with and without chemical
suppression. The associated thermostattable detector block is normally
installed in the 733 IC Separation Center, but can also be used as a
separate detector. The two following versions are available:

• 2.732.0010 IC Detector with standard detector block

• 2.732.0110 IC Detector with metal-free detector block

The 732 IC Detector is operated using the keypad with operator guid­ance via the two-line LCD. In addition to setting of the measurement
parameters, time programs can be generated which can be used to ini­tiate a large number of instrument functions for each of the maximum
20 program steps. Further, the same functions can be executed at a
specific time with 4 programmable "events".

1.1 Instrument description

The 732 IC Detector is equipped with various interfaces for communi­cation purposes. Recorders, integrators or the «IC Metrodata for
Win95» chromatography data system can be connected to the analog
output (1 V or 10 mV) for the plotting and evaluation of chromatograms.
The two RS232 interfaces are used for the connection of a 709 IC
Pump, a printer or a PC for remote control of the IC system. Finally,
programmable signals at a "remote" interface can be employed to con­trol any external devices which, in turn, can start functions at the IC
system.

The 733 IC Separation Center is a thermally and electronically iso­lated wet part which accommodates injectors, columns, detectors,
suppressor module and pulsation dampener and is controlled by the
732 IC Detector. The following versions are available:

• 2.733.0010 IC wet part with 1 injector for a one-channel system

with electronic suppression

• 2.733.0020 IC wet part with 2 injectors for a two-channel system

with electronic suppression

• 2.733.0120 IC wet part with 2 injectors for a two-channel system

with electronic suppression, metal-free

• 2.733.0030 IC wet part with 1 injector and 1 Metrohm

Suppressor Module MSM for a one-channel system
with chemical suppression

• 2.733.0130 IC wet part with 1 injector and 1 Metrohm

732 IC Detector / 733 IC Separation Center

Suppressor Module MSM for a one-channel system
with chemical suppression, metal-free

1

1 Introduction

The 732 IC Detector and 733 IC Separation Center are the main com­ponents of a modular ion chromatography system that can be ex­panded to meet the wishes of the individual user (see Fig. 1). The
minimum configuration of the one-channel system also includes a 709
IC Pump, a separating column and a recorder. The two-channel system
requires at least a second 732 IC Detector and a second 709 IC Pump.
Printers, integrators, data recording devices, PC and autosamplers can
be attached to both systems. Further, practically all HPLC peripherals
and parts available on the market such as precolumns, additional
separating columns, additional detectors and other injection systems
can be seamlessly integrated in the system.

However, the individual IC units can also be freely combined with
common HPLC instruments. This offers the possibility of expanding
your system to a standalone ion chromatograph.

Two-channel systemOne-channel system

PC

IN IN

PL

752

750/766

S

754

W

IF

732

D
M

C

D

733

C

I

I

709 709

WW

S

W

PL

732

750/766

EE

Fig. 1: Block diagram of the ion chromatography system

PRPR

C Separating

column
D Detector PC PC 733 IC Separation Center
E Eluent PL Recorder 750 Autosampler
I Injector PR Printer 752 Pump Unit
IF Interface S Sample 754 Dialysis Unit
IN Integrator W Waste 766 IC Sample Processor

2

M

Suppressor module

(one-channel system only)

709

IC Pump

732

IC Detector

732 IC Detector / 733 IC Separation Center

1.2 Parts and controls

1.2.1 732 IC Detector

88 11 22 33 44

IC Detector732

+0.023 µS/cm 15.2 min
Full Scale 5.00 µS/cm

OVERLOAD

THERMOSTAT

ZERO

PARAM

FULL

SCALE

PROG

R/S

CONFIG

7

ZERO OFF

4

PLOT

1

MARK

0

PUMP R/S

8 9

EVENT

5 6

REPORT

2 3

.

1.2 Parts and controls

METHOD

PROGRAM

PRINT

SELECT

-/+

QUIT ENTER

CLEAR

77 66 55

Fig. 2: Front of the 732 IC Detector

11 Display (LCD)

comprising 2 lines each of 24
characters

22 Main function keys

Auto-zero, Parameters, Full Scale,
Program start/stop

33 Numeric keys

Edit mode: Numeric keys
Basic mode: Function keys

44 Auxiliary function keys

Select, Clear, Quit, Enter

55 Program status display (LED)

LED dark: Program inactive
LED lit up: Program ready
LED flashes: Program running

66 Auto-zero display (LED)

LED dark: Auto-zero switched off
LED lit up: Auto-zero switched on

77 Thermostat display (LED)

LED dark: Heating switched off
LED lit up: Heating switched on

88 Overload display (LED)

LED lit up: Meas. signal >150%

of full-scale range

LED flashes: Meas. signal >180%

of full-scale range

732 IC Detector / 733 IC Separation Center

3

1 Introduction

99

WARNING - Fire Hazard -

For continued protection replace only
with the same type and rating of fuse

Type 1.732.0010 Nr.

f=50-60 Hz

S=70 VA

100-120V:

220-240V:

S

Fuse
0,63A(T)
0,315A(T)

Output

0...1V 0...10mV

Remote

RS 232

Made by Metrohm Herisau Switzerland

1313121211111010

Detector Block

733 IC Separation Center

709 IC Pump

1919

1818 1717 1616 1515 1414

Fig. 3: Rear of the 732 IC Detector

99 Mains switch

switch to switch instrument on and off:

I = ON 0 = OFF

1515 Connection for 709 IC Pump

RS232 interface, can also be used for
connection of a printer

1010 Serial number 1616 RS232 interface

connection of a printer, PC, etc.

1111 Analog output 0……1 V

red socket: live
black socket: common

1212 Analog output 0……10 mV

red socket: live

1717 Remote interface

remote I/O lines for connection of
external devices

1818 Mains connection plug

mains connection, see section 2.4

black socket: common

1313 Connection for detector block 1919 Fuse holder

changing the fuses, see section 2.4

1414 Connection for 733 IC Separation

Center

4

732 IC Detector / 733 IC Separation Center

1.2.2 733 IC Separation Center

IC Separation Center733

1.2 Parts and controls

FILL INJECT

A B

STEP

FILL INJECT

21212020 2222 2424 2525 2626 27272323 2828

Fig. 4: Front of the 733 IC Separation Center

2020 Door to interior 2525 "FILL" key for valve B or "STEP" for

suppressor module

2121 Connection for 6.2816.020 Syringe 2626 "INJECT" key for valve B
2222 Feedthrough for aspirating tubing 2727 733.0010: Feedthrough for capillary

733.0X20: Connection for

6.2816.020 Syringe

733.0X30: Feedthrough for
suppressor inlet cap.

2323 "FILL" key for valve A 2828 733.0010: Feedthrough

733.0X20: Feedthrough for
aspirating tubing

733.0X30: Feedthrough for sup­pressor inlet capillary

2424 "INJECT" key for valve A

732 IC Detector / 733 IC Separation Center

5

1 Introduction

2929 3030 3131 3232 3333

3434

3434

4848

4747

External

Power
24VDC/2A
5VDC/0,5A

Type: 1.733.00X0

Nr.: XX XXX

Pos.

Fill

Integr.

Ground

Start

Fill

Inject

RUN

COM

RUN

COM

732 IC Detector

3434

Waste AWaste B

3535

3434

B

Pos.

Fill

Integr.

Ground

Start

Fill

Inject

RUN

COM

Made by Metrohm Herisau Switzerland

732 IC Detector

RUN

COM

A

3636

3737

4646

4545

3737

3737

3838

Inlet AInlet B

3939

3737

4444 4343 4242 4141 4040

Fig. 5: Rear of the 733 IC Separation Center

6

732 IC Detector / 733 IC Separation Center

1.2 Parts and controls

2929 Opening for detector cable B

opening for connecting cable
detector block B – 732

3030 Opening for outlet capillary B

discharge of the eluent of column B to
waste

3131 Rear panel opening

(closed with plastic stopper) for
additional supply and discharge lines
to and from the inner compartment

3232 Opening for outlet capillary A

discharge of the eluent of column A to
waste

3333 Opening for detector cable A

opening for connecting cable
detector block A – 732

3434 Knurled screw

for fastening the rear panel 3535

3939 Detachable rear panel

access to bottom part of inner
compartment

4040 Opening for inlet capillary A

supply of the eluent for column A

4141 Rear panel opening

(closed with plastic stopper) for
additional supply and discharge lines
to and from the inner compartment

4242 Connection for drain tube

for discharge of spilled liquid from the
inner compartment

4343 Rear panel opening

(closed with plastic stopper) for
additional supply and discharge lines
to and from the inner compartment

4444 Opening for inlet capillary B

supply of the eluent for column B

3535 Detachable rear panel

4545 Connection for 732 IC Detector B

access to top part of the inner
compartment

3636 Connection for 732 IC Detector A 4646 Terminal block for valve B

Ground, Fill, Inject:

inputs for control of the valve

Pos.Fill:

output signal on switching of the
valve to position "FILL"

Integr.Start:

output signal on switching of the
valve to position "INJECT"

3737 Knurled screw

for fastening rear panel 3939

3838 Terminal block for valve A

Ground, Fill, Inject:

inputs for control of the valve

Pos.Fill:

4747 Model plate

with serial number

4848 Connection for external supply

connection of power supply unit
(5 V, 0.5 A / 24 V, 2 A) in operation
without 732 IC Detector

output signal on switching of the
valve to position "FILL"

Integr.Start:

output signal on switching of the
valve to position "INJECT"

732 IC Detector / 733 IC Separation Center

7

1 Introduction

1.3 Information on the Instructions for Use

Please read through these Instructions for Use carefully before you put
the 732 IC Detector and 733 IC Separation Center into operation. The
Instructions for Use contain information and warnings which must be
heeded the user to assure safe operation of the instruments.

1.3.1 Organization

These 8.732.1033 Instructions for Use for the 732 IC Detector and
733 IC Separation Center provide a comprehensive overview of the in­stallation, startup procedure, operation, fault rectification and technical
specifications of these instruments. The Instructions for Use are organ­ized as follows:

Section 1 Introduction

General description of instruments, parts and controls
and safety notes

Section 2 Installation

Installation of 732 IC Detector / 733 IC Separation Cen­ter, attachment of accessories and external devices

Section 3 Operating tutorial

Introduction to the operation using an example

Section 4 Operation

Detailed description of the operation and explanation of
functions of all keys

Section 5 Notes – Maintenance – Faults

Notes on ion chromatography, maintenance, fault rectifi­cation, diagnostic tests, validation

Section 6 Interfaces (green pages)

Description of RS232 interfaces, remote interfaces, valve
interface and analog output

Section 7 Appendix

Technical data, standard equipment, options, warranty,
declarations of conformity, index

To find the required information on the instruments, you will find it an
advantage to use either the Table of contents or the Index at the
back. The 8.732.1043 Quick Reference Guide is suitable for use as a
reference work for daily use as it explains the most important parame­ters and key functions.

As a supplement to the Instructions for Use, the Metrohm Monograph

8.732.2003 "Ion chromatography" is also supplied. This provides an
introduction to the theoretical fundamentals and general information on
separating columns and sample pretreatment. You will find detailed in­formation on the separating columns available from Metrohm and on
special IC applications in the relevant "Application Bulletins", which
are available on request free of charge from your Metrohm agency.

8

732 IC Detector / 733 IC Separation Center

1.3.2 Notation and pictograms

The following notations and pictograms (symbols) are used in these In­structions for Use:

<PARAM> Key

"Range" Parameter or entry value

3535 Part or control of 732/733

2222 Part or control of 709

1.3 Information on the Instructions for Use

>PARAM/detector
range: 1.00 mS/cm

Display

Text in display 11 of the 732 IC
Detector

Hazard

This symbol draws attention to a
possible danger to life or of injury if
the associated directions are not
followed correctly.

Warning

This symbol draws attention to
possible damage to instruments or
instrument parts if the associated
directions are not followed cor­rectly.

Caution

This symbol marks important infor­mation. First read the associated
directions before you continue.

732 IC Detector / 733 IC Separation Center

Comment

This symbol marks additional in­formation and tips.

9

1 Introduction

1.4 Safety notes

1.4.1 Electrical safety

While electrical safety in the handling of the 732 IC Detector and 733
Separation Center is assured in the context of the specifications IEC
1010-1 (protection class 1, degree of protection IP40), the following
points should be noted:

• Mains connection

Setting of the mains voltage, checking the mains fuse and the
mains connection must be effected in accordance with the instruc-

tions in section 2.4.

• Opening the 732 IC Detector

If the 732 IC Detector is connected to the power supply, the instru­ment must not be opened nor must parts be removed from it, other­wise there is a danger of coming into contact with components which
are live. Hence, always disconnect the instrument from all voltage
sources before you open it and ensure that the mains cable is
disconnected from mains connection 18 18 !

• Opening the 733 IC Separation Center

Disconnect connecting cable to the 732 IC Detector from con­nector 1414 before you remove the middle housing panel with con-

nectors.

• Protection against static charges

Electronic components are sensitive to static charging and can be
destroyed by discharges. Before you touch any of the components
inside the 732 IC Detector or 733 IC Separation Center, you should
earth yourself and any tools you are using by touching an earthed
object (e.g. housing of the instrument or a radiator) to eliminate any
static charges which exist.

1.4.2 General precautionary rules

• Handling of solvents

Check all lines of the IC system periodically for possible leaks. Follow
the relevant instructions regarding the handling of flammable and/or
toxic solvents and their disposal.

10

732 IC Detector / 733 IC Separation Center

2 Installation

2.1 Flow chart

The following flow chart provides an overview of all installation work. You
will find more detailed information in the relevant sections.

2.1 Flow chart

Setting upSetting up

733.0X20733.0X20

NoNo

Connecting 733Connecting 733 sect. 2.3.1sect. 2.3.1 Connecting 733Connecting 733 sect. 2.3.2sect. 2.3.2

Installing accessoriesInstalling accessories sect. 2.3.3/4sect. 2.3.3/4

Mains connectionMains connection sect. 2.4sect. 2.4

Connecting 709 IC PumpConnecting 709 IC Pump sect. 2.6sect. 2.6

metal freemetal free

YesYes

PrecolumnPrecolumn

NoNo

sect. 2.2sect. 2.2

YesYes

NoNo

YesYes

PassivationPassivation sect. 2.6.7sect. 2.6.7

Precolumn with twin cartridge holderPrecolumn with twin cartridge holder sect. 2.7.2sect. 2.7.2
Precolumn with cartridge headPrecolumn with cartridge head sect. 2.7.3sect. 2.7.3
IC anion precolumn SUPERSEPIC anion precolumn SUPERSEP sect. 2.7.4sect. 2.7.4

Installing sample loopInstalling sample loop sect. 2.8.2sect. 2.8.2

ConditioningConditioning sect. 2.8.7sect. 2.8.7

Connecting external devicesConnecting external devices sect. 2.9sect. 2.9

732 IC Detector / 733 IC Separation Center

733.0010733.0010 Connecting separating columnConnecting separating column sect. 2.8.4sect. 2.8.4

NoNo

733.0X20733.0X20 Connecting separating columnConnecting separating column sect. 2.8.5sect. 2.8.5

NoNo

733.0X30733.0X30 Connecting sep. col. + suppressorConnecting sep. col. + suppressor sect. 2.8.6sect. 2.8.6

NoNo

YesYes

YesYes

YesYes

11

2 Installation

2.2 Setting up the instruments

2.2.1 Packaging

The 732 IC Detector and 733 IC Separation Center are supplied
together with the separately packed accessories in special packagings
containing shock-absorbing foam linings designed to provide excellent
protection. The actual instruments are packed in an evacuated
polyethylene bag to prevent the ingress of dust. Please store all these
special packagings as only they assure transport of the instruments
free from damage.

2.2.2 Check

After receipt, immediately check whether the shipment is complete and
has arrived without damage (compare with delivery note and list of ac­cessories in section 7.2). In the case of transport damage, see instruc­tions in section 7.4.1 "Warranty".

2.2.3 Location

Position the instruments in the laboratory at a location convenient for
operation, free from vibrations and protected against a corrosive at­mosphere and contamination by chemicals. The same applies to all
other components of the IC system.

To avoid disturbing temperature influences on the insulated column
compartment, the entire system including pump and eluent reservoir
must be protected against direct sunlight.

2.2.4 Arrangement of the instruments

In one-channel operation, the 709 IC Pump, 733 IC Separation Center
and 732 IC Detector are best stacked on top of one another in this or­der.

In two-channel operation (2.733.0X20 IC Separation Center), the opti­mum arrangement (1, 2 or 3 towers) depends on the laboratory space
available. However, the 709 IC Pumps should be set up at the very
bottom and the 732 IC Detectors at the very top.

12

To ensure that the arrangement of pumps and detectors for the two
channels A and B is clearly apparent in two-channel operation, it is
advantageous to mark the instruments. The 6.2248.000 Magnetic
plate is enclosed with the 732 IC Detector for this purpose. It can be
cut to the desired size, labeled (e.g. with "A" or "B") and affixed to the
appropriate instrument.

732 IC Detector / 733 IC Separation Center

2.3 Connection of 733 IC Separation Center

2.3 Connection of 733 IC Separation Center

2.3.1 2.733.0010/2.733.0X30 IC Separation Center

The instrument versions 2.733.0010 and 2.733.0030 of the IC Separa­tion Center are operated with a 732 IC Detector whose standard
equipment also includes the 1.732.0100 Detector block. For the
metal-free 2.733.0130 instrument version the metal-free 1.732.0110
Detector block must be used. It is best to proceed as follows when
connecting the two instruments and the detector block:

1 Install detector block

• Unscrew the four knurled screws 3434 from the top rear panel

3535 of the 733 IC Separation Center and remove rear panel

(see Fig. 5).

• Position detector block from the back in the space provided

in the 733 IC Separation Center on the right and push fully to
the front (see Fig. 16).

• Insert the cable permanently attached to the detector block in

opening 3333 and the outlet capillary in opening 3232 "Waste A" of
the rear panel 3535.

• Replace rear panel 3535 and screw to the 733 IC Separation

Center using the four knurled screws 3434.

2 Connect detector block

• Plug the gray connecting cable permanently attached to the

detector block into connection 1313 "Detector Block" of the 732
IC Detector and fasten to the instrument by tightening the
screws in the cable connector (see Fig. 6).

Cable from
detector block

6.2125.090

RUN

COM

Fig. 6: Connection 732 – 2.733.0010/2.733.0X30

732 IC Detector / 733 IC Separation Center

13

2 Installation

3 Connect waste container

• Lead the outlet capillary of the detector block to a sufficiently

large waste container and fix in place.

4 Connect 732 to 733

• Plug one end of the 6.2125.090 Connecting cable into con-

nection 1414 "733 IC Separation Center” of the 732 IC Detector
and fasten to the instrument by tightening the screws in the
cable connector (see Fig. 6).

• Plug the other end of the 6.2125.090 Connecting cable into

connection 3636 "732 IC Detector" of the 733 IC Separation
Center and fasten to the instrument by tightening the screws
in the cable connector (see Fig. 6).

2.3.2 2.733.0X20 IC Separation Center

For operation of the 2.733.0020 or the metal-free 2.733.0120 instrument
version of the 733 Separation Center, two 1.732.0100 or metal-free

1.732.0110 IC Detectors are needed. It is best to proceed as follows
when connecting the instruments and the two detector blocks:

1 Install detector blocks

• Unscrew the four knurled screws 3434 from the top rear panel

3535 of the 733 IC Separation Center and remove rear panel

(see Fig. 5).

• Position first detector block A from the back in the space

provided in the 733 IC Separation Center on the right and
push fully to the front (see Fig. 16).

• Position second detector block B from the back in the space

provided in the 733 IC Separation Center on the left and push
fully to the front (see Fig. 16).

• Insert the cable permanently attached to detector block A in

opening 3333 and the outlet capillary in opening 32 32 "Waste A"
of the rear panel 3535.

• Insert the cable permanently attached to detector block B in

opening 2929 and the outlet capillary in opening 3030 "Waste B"
of the rear panel 3535.

• Replace rear panel 3535 and fasten to 733 IC Separation Center

with the four knurled screws 3434.

2 Connect detector blocks

• Plug the gray connecting cable permanently attached to

detector block A into connection 1313 "Detector block" of the
first 732 IC Detector and fasten to the instrument by tighten­ing the screws in the cable connector (see Fig. 7).

• Plug the gray connecting cable permanently attached to

detector block B into connection 1313 "Detector Block" of the
second 732 IC Detector Block and fasten to the instrument by
tightening the screws in the cable connector.

14

732 IC Detector / 733 IC Separation Center

2.3 Connection of 733 IC Separation Center

3 Connect waste container

• Lead the outlet capillaries of the two detector blocks to a

sufficiently large waste container and fix in place.

4 Connect 732 to 733

• Plug one end of the 6.2125.090 Connecting cable into con-

nection 1414 "733 IC Separation Center“ of the first 732 IC De-
tector and fasten to the instrument by tightening the screws
in the cable connector (see Fig. 7).

• Plug other end of the 6.2125.090 Connecting cable into

connection 3636 "732 IC Detector" of the 733 IC Separation
Center and fasten to the instrument by tightening the screws
in the cable connector.

• Plug one end of the 6.2125.090 Connecting cable into con-

nection 1414 "733 IC Separation Center" of the second 732 IC
Detector and fasten to the instrument by tightening the
screws in the cable connector.

• Plug the other end of the 6.2125.090 Connecting cable into

connection 4545 "732 IC Detector" of the 733 IC Separation
Center and fasten to the instrument by tightening the screws
in the cable connector.

Cable from
detector block

Cable from
detector block

6.2125.0906.2125.090

COM

RUN

732 IC Detector / 733 IC Separation Center

Fig. 7: Connection 732 – 2.733.0X20

15

2 Installation

2.3.3 Connection of syringe and aspirating tubing

For manual filling of the sample loops mounted on the injection valves,
the 6.2816.020 Syringe and the PTFE aspirating tubing already screwed
to the valve are needed. These accessories are mounted or adjusted as
follows:

1 Connect syringe

• Push 6.2816.020 Syringe (without needle) as far as it will go

into connection socket 2121 (for valve A) or 2727 (for valve B)
(see Fig. 4).

2 Adjust aspirating tubing

• Loosen the rotary nipple screwed onto the interior side of

connection 2222 or 2828.

• Pull PTFE aspirating tubing 8888 (see Fig. 16 and Fig. 17) by

hand out of connection 2222 or 2828 as far as desired.

• Retighten rotary nipple on the interior side of connection 2222

or 2828 to fix the aspirating tubing in place.

2.3.4 Connection of the drain tube

The 733 IC Separation Center has a connection at the rear to which a
drain tube for discharged liquids can be attached. Proceed as follows:

1 Connect drain tube

• Mount 6.1816.00 Silicone tubing on connection nipple 4242
(see Fig. 5).

2 Lead drain tube to collecting vessel

• Lead the other end of the drain tube to a suitable collecting
vessel and fix in place.

2.3.5 Connection of the 6.5324.000 Bottle rack (option)

The optional available 6.5324.000 Bottle rack for supply vessels can be
placed on top of the IC system tower. The accessories include the
supply vessels for eluent (2 L), regeneration solution (1 L) and rinsing
solution (1 L). For the connection of the supply capillaries leading to the
709 IC Pump and the suppressor module, see the instructions given on
the enclosed leaflet.

16

732 IC Detector / 733 IC Separation Center

2.4 Mains connection

Follow the instructions below for connecting to the power supply. If
the instrument is operated with a mains voltage set wrongly and/or
wrong mains fuse, there is a danger of fire!

2.4.1 Setting the mains voltage

Before switching on the 732 IC Detector for the first time, check that the
mains voltage set on the instrument (see Fig. 8) matches the local
mains voltage. If this is not the case, you must reset the mains voltage
on the instrument as follows:

1 Disconnect mains cable

Disconnect mains cable from mains connection plug 1818 of the
732 IC Detector.

2.4 Mains connection

2 Remove fuse holder

Using a screwdriver, loosen fuse holder 1919 below the mains
connection plug 1818 and take out completely.

3 Check and change fuse if necessary

Carefully take the fuse installed for the desired mains voltage out
of fuse holder 1919 and check its specifications (the position of the
fuse in the fuse holder is marked by the white arrow imprinted
next to the mains voltage range):

100……120 V 0.63 A (slow-blow) Metrohm No. U.600.0014
220……240 V 0.315 A (slow-blow) Metrohm No. U.600.0011

4 Insert fuse

Change fuse if necessary and reinsert in fuse holder 1919.

5 Install fuse holder

Depending on the desired mains voltage, insert fuse holder 1919 in
the 732 IC Detector so that the corresponding mains voltage
range can be read normally and the adjacent white arrow points
to the white bar imprinted below the fuse holder (see Fig. 8).

732 IC Detector / 733 IC Separation Center

17

2 Installation

100 – 120 V220 – 240 V

2.4.2 Fuses

99

1818

99 Mains switch

1818 Mains con-

nection plug

1919

220 -- 240 V

100 -- 120 V

100 -- 120 V

220 -- 240 V

1919 Fuse holder

Fig. 8: Setting the mains voltage

One of the two fuses 0.63 A/slow-blow for 100…120 V or 0.315 A/slow­blow for 220…240 V is installed in fuse holder 1919 of the 732 IC Detector
as standard.

Ensure that the instrument is never put into operation with fuses of
another type, otherwise there is danger of fire!

For checking or changing fuses, process as described in section 2.4.1.

2.4.3 Mains cable and mains connection

Mains cable

The instrument is supplied with one of three mains cables

• 6.2122.020 with plug SEV 12 (Switzerland, …)

• 6.2122.040 with plug CEE(7), VII (Germany, …)

• 6.2133.070 with plug NEMA 5-15 (USA, …)

which are three-cored and fitted with a plug with an earthing pin. If a
different plug has to be fitted, the yellow/green lead (IEC standard)
must be connected to protective earth (protection class 1).

Any break in the earthing inside or outside the instrument can make it
a hazard!

Mains connection

Plug the mains cable into mains connection plug 1818 of the 732 IC De-
tector (see Fig. 8).

2.4.4 On/off switching of the instruments

18

The 732 IC Detector is switched on and off using mains switch 99. When
the instrument is switched on, display 11 lights up.

732 IC Detector / 733 IC Separation Center

2.5 Capillary connections

2.5.1 Capillaries

Some of the connections under high pressure between the feed pump
and the detector block must be set up by the user. For metal-free sys-
tems, the 6.1831.010 PEEK capillary (i.d. = 0.25 mm, e.d. = 1/16",
length = 3 m) must be used.

For non metal-free systems, the 6.1831.010 PEEK capillary can also
be used in the pressure range of 0…25 MPa (0…250 bar); on the other
hand, in the pressure range of 25…50 MPa (250…500 bar), which is
permissible only together with the non metal-free 2.709.0010 version of
the 709 IC Pump, the 6.2620.020 Steel capillary (i.d. = 0.25 mm,
e.d. = 1/16", length = 3 m) must be used.

With PEEK capillaries the connection is made preferably with the

6.2744.010 PEEK connectors, with steel capillaries, the connection is
made preferably with 6.2620.000 and 6.2620.010 steel connectors (see
section 2.5.2 and section 2.5.3).

2.5 Capillary connections

Capillaries fitted with new connectors must have a perfectly flat cut
surface. For PEEK capillaries it is best to use the 6.2621.080 Capil-

lary tubing cutter, for steel capillaries the 6.2621.040 Capillary
tubing cutter (both available as an option).

2.5.2 Steel connectors

For the connection of steel capillaries, the steel connectors 6.2620.010
Ferrule and 6.2620.000 Pressure screw available as an option can

be used. Proceed as follows:

1 Mount connectors

Slide a pressure screw 5050 (6.2620.000) and a ferrule 4949
(6.2620.010) over the end of the capillary 5151 to be fastened as
shown in Fig. 9.

2 Insert capillary in connection

Push capillary end into the corresponding connection as far as it
will go (to avoid dead volume).

3 Tighten compression fitting

Tighten pressure screw 5050 with the open-end spanner 1/4"
(6.2621.050) supplied.

For the connection of capillaries to the injection valves, use only the
special steel connections contained in a plastic bag affixed to the
valve (or as an alternative the 6.2744.010 PEEK compression fittings).
If other steel connectors are used (e.g. 6.2620.000 and 6.2620.010),
the valve connection may be damaged!

732 IC Detector / 733 IC Separation Center

19

2 Installation

50504949 5151 5252

4949 Ferrule (6.2620.010)
5050 Pressure screw (6.2620.000)
5151 Capillary

6.2620.020 Steel capillary or

6.1831.010 PEEK capillary

Fig. 9: Connectors for capillaries

2.5.3 PEEK connectors

For the connection of 6.1822.010 PEEK capillaries (i.D. = 0.3 mm) or

6.1822.010 PTFE microcapillaries, the 6.2744.010 PEEK compres-
sion fittings are used. Proceed as follows:

1 Mount compression fitting

Slide a compression fitting 5252 (6.2744.010) over the end of the
capillary 5151 to be fastened as shown in Fig. 9.

2 Insert capillary in connection

Push capillary end in the corresponding connection as far as it
will go (to avoid dead volume).

5252 Compression fitting

(6.2744.010)

3 Tighten compression fitting

Tighten compression fitting 5252 by hand (never use tools).

20

732 IC Detector / 733 IC Separation Center

2.6 Connection of 709 IC Pump

2.6.1 Electrical connection

For operation of the 732 IC Detector and 733 IC Separation Center you
can use any commercial HPLC pump. However, as the attainable sen­sitivity depends to a large extent on the quality of the pump, Metrohm
advises use of the 709 IC Pump, which has been specially developed
for the demands of ion chromatography and has minimal pump pulsa­tion and an outstanding flow constancy.

Startup and operation of the 709 IC Pump are described in the 709
Instructions for Use. The eluent, which must be degassed and filtered

(cf. section 5.1.3), is selected on the basis of the separating column in­stalled in the 733 IC Separation Center and the current separation
problem (see 8.732.2003 IC Monograph).

The connection of the 709 IC Pump at the connection 1515 of the 732
IC Detector is shown in Fig. 10. For this you can use the 6.2125.060
Cable available as an option or another RS cable specified as a "null
modem" cable. To ensure proper functioning of the communication
between the 732 IC Detector and 709 IC Pump, the sliding switch 3636 on
the IC pump must be set to "RS 232" and the external control switched
on with key 88 <EXT.> (see 709 Instructions for Use).

2.6 Connection of 709 IC Pump

732

709

Fig. 10: Connection of 709 IC Pump

2.6.2 Pulsation dampener

To protect the column material against pressure drops caused by the
injector, the use of a pulsation dampener connected between the pump
and the injection valve of the 733 IC Separation Center is recom­mended. The optional 6.2620.150 Pulsation dampener MF (see sec-
tion 7.3.1) is very well suited to this purpose.

6.2125.060

The metal-free 6.2620.150 Pulsation Dampener is supplied fully as­sembled and has two connections for capillaries, for which either the
connectors supplied or two 6.2744.010 PEEK compression fittings can
be used. The flow direction is arbitrary. The pulsation dampener is po­sitioned in the interior of the 733 IC Separation Center on the base be­low the injection valve (see Fig. 16 and Fig. 17).

732 IC Detector / 733 IC Separation Center

21

2 Installation

2.6.3 Filter unit PEEK

The 6.2821.100 Filter unit PEEK (see Fig. 11) supplied with the 709
IC Pump is installed between the 709 IC Pump and the injection valve
at the 733 IC Separation Center. This filter unit serves to avoid contami­nation of the piston seals of the 709 IC Pump by abrasive particles and
can be used in the pressure range 0…25 MPa (0…250 bar).

The two filter units PEEK supplied with the 2.733.0X30 IC Separation
Center (with suppressor) are installed between the 752 Pump Unit and
the inlet capillaries for regeneration and rinsing solution. These filter
units serve to protect the suppressor module from foreign particles and
bacterial growth.

The 6.2821.100 Filter unit PEEK consists of the housing 5454 and the two
connectors 5353 (with filter) and 5555 (without filter) screwed into the hous-
ing 5454. For the connection of capillaries 5151 PEEK compression fittings
5252 (6.2744.010) must be used. New connectors 5353 with filter are avail­able as an option with the ordering number 6.2821.110 (set of 10).

For the connection of the filter unit, please note the flow direction
arrow printed on the housing.

5151 5252 5353 5454 5555 5252 5151

Fig. 11: 6.2821.100 Filter unit PEEK

5151 Capillary

6.1831.010 PEEK capillary

5252 Compression fitting (6.2744.010) 5555 Connector without filter

5353 Connector with filter (6.2824.110)

Part of 6.2824.100 Filter unit

22

5454 Housing for filter unit

Part of 6.2824.100 Filter unit

Part of 6.2824.100 Filter unit

732 IC Detector / 733 IC Separation Center

2.6.4 Filter unit Manufit

The optional 6.2821.000 Filter unit Manufit (see section 7.3.1) is in­stalled between the 709 IC Pump and the injection valve at the 733 IC
Separation Center. The filter unit serves to avoid contamination of the
piston seals by abrasive particles and can be used in the pressure
range 0…50 MPa (0…500 bar) for non metal-free systems together
with steel capillaries. It is installed as follows (see Fig. 12):

1 Prepare Manufit housing

• Insert outlet capillary 6363 with steel mesh holding end 6161 into

Manufit housing 6262.

• Insert the 4 steel meshes 6060 provided into the steel mesh

holding end 6161.

• Press the PTFE gasket 5959 into the steel mesh holding end

6161.

2 Prepare Manufit pressure screw

• Insert inlet capillary 5656 with counterpart end 5858 into Manufit

pressure screw 5757.

2.6 Connection of 709 IC Pump

5656

5757

5858

5959

6060

6161

3 Assembly

• Fit the two capillary end pieces 5858 and 6161 together.

• Screw Manufit pressure screw 5757 and Manufit housing 6262

firmly together.

To replace contaminated steel meshes, proceed in the reverse order.

IC Pump
709

5656 Inlet capillary
5757 Manufit pressure screw
5858 Counterpart end
5959 PTFE Gasket (6.2821.010)
6060 Steel meshes (6.2821.020)
6161 Steel mesh holding end

6262

IC Separation

6363

Fig. 12: 6.2821.000 Filter unit Manufit

732 IC Detector / 733 IC Separation Center

Center 733

6262 Manufit housing
6363 Outlet capillary

23