Leica M205 FA User Manual

Leica M165 FC
Leica M205 FA
User Manual

General Instructions

Safety concept

Before using your microscope for the first time,
please read the "Safety concept" brochure
included with your instrument. It contains addi­tional information about handling and care.

Use in clean rooms

The Leica M series can be used in clean rooms
without any problems.

Cleaning

ρ

Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.

ρ

Never use chemicals to clean colored
surfaces or accessories with rubberized
parts. This could damage the surfaces,
and specimens could be contaminated by
abraded particles.

ρ

In most cases, we can provide special solu­tions on request. Some products can be
modified, and we can offer other accesso­ries for use in clean rooms.

Servicing

ρ

Repairs may only be carried out by Leica
Microsystems-trained service technicians.
Only original Leica Microsystems spare
parts may be used.

Responsibilities of person in charge of
instrument

ρ

Ensure that the Leica stereomicroscope
is operated, maintained and repaired by
authorized and trained personnel only.

Leica M-series User Manual 2

Important Safety Notes

User Manual

The individual modules of the Leica M stereo­microscopy series include an interactive
CD-ROM with all relevant user manuals in
20 other languages. Keep it in a safe place,
and readily accessible to the user. User manu­als and updates are also available for you
to download and print from our web site at
www.stereomicroscopy.com.

This User Manual describes the special func­tions of the individual modules of the Leica M
stereomicroscopy series and contains impor­tant instructions for their operational safety,
maintenance, and accessories.

The "Safety concept" booklet contains addi­tional safety information regarding the service
work, requirements and the handling of the
stereomicroscope, accessories and electrical
accessories as well as general safety instruc­tions.

You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.

Before installing, operating or using the instru­ments, read the user manuals listed above.
In particular, please observe all safety instruc­tions.

To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.

Special manuals

Special manuals are provided for a number of
accessories:

ρ

User Manual M2-216-1 for lamp housing
with high-pressure mercury burner.

ρ

User Manual for ebq 100 isolated supply
unit for 100W high-pressure mercury
burners.

ρ

User Manual M2-267-1 for the motorized
focus system.

ρ

User Manual M2-166-2 for FluoCombi III™.

Leica M-series User Manual 3

Symbols Used

Warning of a danger

This symbol indicates especially impor-

•

tant information that must be read and

complied with.

Failure to comply can cause the following:

ρ

Hazards to personnel

ρ

Functional disturbances or damaged instru­ments

Warning of hazardous electrical voltage

This symbol indicates especially impor-



tant information that is mandatory to

read and observe.

Failure to comply can cause the following:

ρ

Hazards to personnel

ρ

Functional disturbances or damaged instru­ments

Danger due to hot surface

This symbol warns against touching



accessible hot surfaces, e.g. those of

light bulbs.

Important information

This symbol indicates additional infor-



mation or explanations that intend to

provide clarity.

Explanatory notes

ρ

This symbol within the text stands for addi­tional information and explanations.

Figures

(1) Numbers in parentheses within the

descriptions relate to the figures and the
items within those figures.

Leica M-series User Manual 4

Safety Instructions

Description

The individual modules fulfill the highest
requirements for observation and documenta­tion of Leica stereomicroscopes of the M series.

Intended use

Refer to "Safety Concept" booklet ▶

Non-intended use

Refer to "Safety Concept" booklet ▶

Never use M series microscopes or their compo­nents for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.

The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that

are outside of the scope of this manual.

Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.

Place of use

Refer to "Safety Concept" booklet ▶

ρ

Electrical components must be placed at
least 10 cm away from the wall and from
flammable substances.

ρ

Avoid large temperature fluctuations,
direct sunlight and vibrations. These condi­tions can distort measurements and micro­graphic images.

ρ

In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.

Responsibilities of person in charge of
instrument

Refer to "Safety Concept" booklet ▶

Ensure that:

ρ

The M series stereomicroscopes and
accessories are operated, maintained and
repaired by authorized and trained person­nel only.

ρ

All operators have read, understood and
observe this User Manual, and particularly
the safety instructions.

Leica M-series User Manual 5

Safety Instructions (cont'd.)

Repairs, service work

Refer to "Safety Concept" booklet ▶

ρ

Only original Leica Microsystems spare
parts may be used.

ρ

Before opening the instruments, switch off
the power and unplug the power cable.

Touching the live circuit can cause



injury.

Transport

ρ

Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.

ρ

In order to prevent damage from vibrations,
disassemble all moving parts that (accord­ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.

Integration in third-party products

Refer to "Safety Concept" booklet ▶

Disposal

Refer to "Safety Concept" booklet ▶

Legal requirements

Refer to "Safety Concept" booklet ▶

EC Declaration of Conformity

ρ

Refer to "Safety Concept" booklet

Health risks

Workplaces with stereomicroscopes

•

facilitate and improve the viewing task,
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For

this reason, appropriate measures for reduction
of the workload must be taken:

ρ

Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).

ρ

Thorough training of the personnel, giving
consideration to ergonomic and organiza­tional aspects.

ρ

The ergonomic design and construction
of the Leica M stereomicroscopy series are
intended to reduce the exertion of the user
to a minimum.

Leica M-series User Manual 6

Safety Instructions (cont'd.)

Direct contact with eyepieces is a poten-

•

tial transmission method for bacterial

and viral infections of the eye.

The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.

Light sources: Safety regulations

Protective measures of the manufacturer:

ρ

UV protection screen in front of the speci­men plane prevents the user from looking
directly into the UV rays.

ρ

Dummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.

ρ

UV filters are installed in the observation
beam paths to protect the eyes.

ρ

The stray-light protection on the lamp
housing prevents irradiation of the hands.

Warning

UV radiation could damage the eyes.

•

Therefore:

ρ

Never look into the light spot on the speci­men plane without a UV protection screen.

ρ

Never look into the eyepieces if no excita­tion filter is in the beam path.

ρ

Fill empty filter positions with dummy filter
carriers (e.g. M165 FC).

ρ

Do not select a white, strongly reflective
background for the specimen.

Leica M-series User Manual 7

Safety Instructions (cont'd.)

Supply unit

Always unplug the supply unit from the power
supply:

ρ

When installing and disassembling the
lamp housing

ρ

Before opening the lamp housing

ρ

When replacing the high-pressure mercury
lamp and other parts, such as the heat­absorbing filter or the collector

ρ

During maintenance work on the supply
unit

Lamp housing

Never open the lamp housing while the

•

lamp is switched on. Risk of explosion,
UV exposure, blinding!

Before opening the lamp housing, allow

•

it to cool off for at least 15 minutes.
Danger of explosion!

Never cover the air duct on the lamp

•

housing. Danger of fire!

Mercury lamp

ρ

Follow the user manual and safety instruc­tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.

ρ

For transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.

ρ

When it has reached the end of its rated life
(follow the manufacturer's specifications
and the minute meter on the supply unit).

ρ

To minimize the risk of explosion, replace a
discolored mercury lamp promptly.

ρ

Leica Microsystems assumes no liability
for damage caused by exploding, incor­rectly installed or improperly used mercury
lamps.

Leica M-series User Manual 8

Contents

General Instructions 2
Important Safety Notes 3
Symbols Used 4
Safety Instructions 5

The Leica M Series for Fluorescence

Congratulations! 13
A Step Towards Infinity 14
The Electronics: Comfort, Convenience and Safety 15
The Modular Design: Everything is Relative 16
Maximum Compatibility 17
On We Go 18

Assembly

Assembling the Focusing Column for MDG Bases 20
Assembling the Focusing Column with an Incident-light Base 21
Motorized Focus: Restricting the Travel Path 22
Optics Carrier 23
Tube 24
Eyepieces 25
Objective 26
UV Protection Screen – Assembly 27
Objective Nosepiece – Assembly 28
Objective Nosepiece – Adjusting Parfocality 29

Transmitted-light Base TL ST 30
FluoCombi III™ – Preparations 31
FluoCombi III™ – Assembly 32
FluoCombi III™ – Adjusting the Parfocality 34
FluoCombi III™ – Centering the Objectives 36
FluoCombi III™ – Filter and UV Protection Screen 37
Transmitted-light Base TL BFDF: Before First Use 38
Transmitted-light Base TL BFDF 39
TL RC™ / TL RCI™ 40
IsoPro™ Manual Mechanical Stage: Assembly 41
Leica IsoPro™ Motorized Mechanical Stage: Assembly 44
Cables: Connections 48
Cables: Cable Duct 49
Cables: Diagram 50
Leica LED5000 MCI™ 51
Leica LED5000 MCI™: Alternative Assembly 52
Leica LED5000 RL 53
Leica EL6000 – Safety Notes 54
Leica EL6000 – Preparations 57
Leica EL6000 – Connection to the Fluorescence Lamp Housing 59
Leica EL6000 – Replacing the Lamp 61
Leica EL6000 – Connection to the Fluorescence Lamp Housing (cont'd.) 62

Leica M-series User Manual 9

Quick Start Guide

The Fastest Route to Success 64
Overview of an M Series Microscope 65
The Correct Interpupillary Distance 66
Using the Eyepieces 67
Focusing 68
Adjusting the Resistance of the Focus Drive 69
Changing Magnification (Zoom) 70
Ratchet Steps and Magnification Levels 71
Parfocality: More Comfort and Convenience for Your Work 72
Iris Diaphragm 73

Eyepieces

Magnification Factors of the Eyepieces 75
Health Notes 76
Dioptric Correction 77
Dioptric Correction and Parfocality 78
Graticules 79

Photography & Video

Photography & Video 81
Photo Tubes and C-mounts 82
Trinocular Video/Phototube 50% 83
Trinocular Video/Phototube 100% 84

Microscope Carrier

The Objective Nosepiece 86

Objectives andOptical Accessories

The Different Types of Objectives 88

Bases

Leica TL ST Transmitted-light Base: Controls 90
Leica TL ST Transmitted-light Base: Operation 91
Leica TL ST Transmitted-light Base: Changing Bulbs 92
Leica TL BFDF Transmitted-light Base: Controls 93
Leica TL BFDF Transmitted-light Base: Operation 94
Leica TL RC™ / TL RCI™: Controls 95
Leica TL RCI™: The Deflection Mirror 96
Leica TL RCI™: Color Intensity and Temperature 97
Leica TL RC™ / TL RCI™: Operation 98
Leica TL RCI™: Methods in Transmitted Light 99
Leica TL RCI™: Relief Images 100
Using Filters 102
Leica IsoPro™ (Non-motorized): Controls 103
Leica IsoPro™ (Motorized): Controls 104

Leica M-series User Manual 10

System Illumination

Leica LED5000 MCI™ 106
Leica LED5000 RL 108
Leica EL6000 – About the Instrument 109
Leica EL 6000 – Operation 111
Leica EL 6000 – Troubleshooting 112
Cleaning and Maintenance 113

Fluorescence

Filter Changer 115
FIM – Fluorescence Intensity Manager 116
About Fluorescence Microscopy 117
Rapid Filter Changers and Filter Types 118
Simple filter holders 119
Equipping the Filter Changer 120
Observation without Fluorescence 121
Double Iris Aperture 122
Commissioning the Fluorescence System 123

Accessories

Leica SmartMove 125
Leica SmartTouch™ 126
Leica PSC Controller 127

Dimensional Drawings

Leica M165 FC 129
Leica M205 FA 132
Leica LED5000 MCI 135
Leica LED5000 RL 136
MATS TL 137

Specications

Leica M165 FC / Leica M205 FA 139
Leica EL6000 – Specifications 143
Leica TL ST Transmitted-light Base 145
Leica TL BFDF Transmitted-light Base 146
Leica TL RC™ / TL RCI™ 147
Leica IsoPro™ Motorized XY Stage 148

Appendix

Calculating the Total Magnification and Field of View Diameter 150
Care, Maintenance, Contact Persons 151

Leica M-series User Manual 11

The Leica M Series for Fluorescence

Leica M-series User Manual 12

Congratulations!

Congratulations on obtaining your new Leica M series stereomicroscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.

The Leica M series embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the M series
adapts perfectly to your needs—no matter which accessories you require
for your tasks.

The entire imaging system, including the zoom, objective and ErgoTube®,
is apochromatically corrected with much technological effort. Contrast,
sharpness, richness in detail, resolution, image and color fidelity are opti­mum. In addition, the patented illumination beam path guarantees at every
zoom level that light utilization is at a maximum and that fluorescence images
are intensely luminous on a jet black background.

Though the reliability and robustness of Leica stereomicroscopes is
legendary, like any high-tech product, the Leica M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.

We wish you the best of success in your work—after all, you are now
equipped with the best tool!

Leica M-series User Manual 13

A Step Towards Infinity

Ever since their introduction by Horatio S.
Greenough, stereomicroscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engi­neers have worked to push magnification, reso­lution and image fidelity to the limit permitted
by optics.

In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimen­sional image seen by the observer becomes
distorted—a sphere becomes an ellipse, a flat
surface curves towards the observer.

Limits are made to be broken

The Leica M205 FA is the world's first fluores­cence stereomicroscope with a zoom range
of 20.5:1. This accomplishment, however, was
not enough for Leica's engineers. With the new
FusionOptic™ in the Leica M205 FA, they have
succeeded in going yet another step beyond
previous limits. In addition to the increase in
magnification, the resolution, too, has been
increased to up to 1050 lp / mm, which corre­sponds to a resolved structure size of 472 nm.

Of course, this increase in performance benefits
your everyday work. Set up your specimens on
the microscope table with comfortable freedom
of movement and discover details in stereomi­croscopy that you could never see before.

* Leica M205 FA with 1.0× planapochromat and 10× eyepieces

Leica M-series User Manual 14

The Electronics: Comfort, Convenience and Safety for Your Experiments

Never before have electronics been used as
extensively in a Leica series as in the new M
series lineup. Optics carrier, stand, base and
illuminator are all connected using electrical
contacts—which provides a number of advan­tages.

Contacts not only transmit data, but also supply
the power.

Reliability for your experiments

Exact reproducibility of results is an increas­ingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnifica­tion, the illumination, the position of the iris

Leica Application Suite (LAS) evaluates the
transmitted data and can restore the test situa­tion later.

diaphragm and more and transmits the infor­mation to the Leica LAS software. Thus you
always know the conditions under which an
image was acquired.

Fewer cables

A large part of the cables have been routed in
the interior of the column. Data are also trans­ferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables—this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.

Leica M-series User Manual 15

The Modular Design: Everything is Relative

The Leica M series provides maximum flexibil­ity in choosing equipment, thanks primarily to
the modular configuration and the compatibil­ity that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.

Despite this, you will notice that the controls
and individual components do not differ signif­icantly. Whichever configuration you choose,
you will quickly feel right at home.

Have a special request? Let us know!

Leica Microsystems enjoys an exceptional repu­tation when it comes to devising customer­specific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.

Leica M-series User Manual 16

Maximum Compatibility

Leica engineers were careful to ensure that the
new Leica M series—like its predecessors—
remains compatible with existing series. This
means that objectives, bases, tubes and so on
can be reused.

Objectives

All new objectives of the Leica M series are
parfocal, meaning that when used with the
objective nosepiece, they can be replaced while
keeping the specimen in perfect focus.

If you prefer, you can continue to use the previ­ous Leica objective series. In this case, parfocal­ity is no longer guaranteed.

Tubes

The interface between the optics carrier and the
tube has remained the same, so existing tubes
fit the new M series. The new tubes are designed
for eyepieces with field number 23, while the
predecessor models were only designed for field
number 21, resulting in a smaller object field.

Eyepieces

However, the new M-series eyepieces have an
audible and tangible click to provide immedi­ate feedback in case of accidental adjustment.

Leica M-series User Manual 17

On We Go

If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the instal­lation instructions and go directly to the Quick
Start Guide on Page 63.

If, on the other hand, you are assembling
the microscope yourself, continue with the
"Assembly" chapter, which begins on Page 19.

For everything you need to know about the
correct use of fluorescence-related parts, refer to
Page 114.

Leica M-series User Manual 18

Assembly

Leica M-series User Manual 19

Assembling the Focusing Column for MDG Bases

The first step is to connect the focusing column
of the M series to the corresponding base.

Tools used

ρ

Hex socket screwdriver, 3 mm

Assembling the column adapter

1. Securely install the column adapter on the

column using the four included screws.

Assembling the focusing column

2. Securely screw the focusing column to the
base using the six included screws.

Leica M-series User Manual 20

Assembling the Focusing Column with an Incident-light Base

When using an incident-light base, the focus­ing column and motorized focus are installed
directly on the base; no extension plate is
required.

Tools used

ρ

Hex socket screwdriver, 3 mm

Assembly

1. Place the focusing column on the side.

2. Insert the four screws provided into the
outer holes of the base.

3. Screw the base securely onto the focusing
drive.

Leica M-series User Manual 21

Motorized Focus: Restricting the Travel Path

Depending on the work situation, it is



useful to restrict the maximum travel
path of the stereomicroscope. This prevents
injury during handling of the sample caused
by fingers or hands becoming trapped or the
specimen touching the objective or even being
damaged by it.

Readjusting the motorized focus

The motorized focus is factory-adjusted and
normally does not need to be readjusted—
even if the maximum travel path is changed.

Exception: If the power fails while the

•

motorized focus is moving, the position
data are lost. In this case, the calibration must
be repeated using the Leica LAS software or the
Leica SmartTouch™. To do so, please consult the
respective manual.

Restricting the bottom travel range

1. Move the motorized focus into the lowest
position you want to reach.

2. Unscrew the screw of the limit stop on the
side of the focusing column.

3. Push the limit stop to the height of the
motorized focus.

It is easiest to move the limit stop by keeping the
screwdriver inserted and moving it upwards.

4. Tighten the screw of the limit stop.

Leica M-series User Manual 22

Optics Carrier

Tools used

ρ

Hex socket screwdriver, 4 mm

Assembling the optics carrier

1. Place the optics carrier on the focusing
column so that the screw fits into the thread
provided and the lug fits into the groove.

2. Press the optics carrier backwards to the
focusing column and screw it in place using
your other hand.

Leica M-series User Manual 23

Tube

All intermediate tubes that fit between the
optics carrier and the binocular tube are fitted
in the same manner.

Tools used

ρ

No tools required.

Preparations

1. Unscrew the positioning screw and remove
the protective cover.

Assembling the tube

2. Push the tube (for example, the inclined
binocular tube) into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.

3. While holding the tube only slightly, care­fully tighten the positioning screw. It is
automatically brought to the correct posi­tion.

Leica M-series User Manual 24

Eyepieces

Tools used

ρ

No tools required.

Magnification range

You can extend the overall magnification range
using available 10×, 16×, 25× and 40× wide­field eyepieces for persons wearing glasses.

Preparation

1. If you want to use an optional graticule,
insert it now (Page 79).

2. Remove the plastic tube guard.

Inserting the eyepieces

3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.

4. Securely tighten the clamping screws.

Leica M-series User Manual 25

Objective

Tools used

ρ

No tools required.

Preparation

1. Remove the protective cap on the optics
carrier by turning it.

Attaching the objective

Hold the objective firmly during assem-

•

bly and disassembly so that it does not
fall onto the stage plate. This applies particu­larly to the 2x planapochromatic objective,
which is very heavy. Remove all specimens from
the stage plate first.

2. Screw the objective clockwise into the

optics carrier.

Alternative fastening options

ρ

If using the objective nosepiece, read the
instructions on Page 28.

ρ

If using the Leica FluoCombi III™, read the
instructions on Page 31.

Leica M-series User Manual 26

UV Protection Screen: Assembly

Tools used

ρ

Allen key

Intended use

The UV protection screen in front of the speci­men plane prevents the user from looking
directly into the UV rays.

Safety Notes

UV radiation can damage the eyes.

•

Therefore, it is mandatory to install the

UV filter and adjust it correctly.

Always position the UV protection

•

screen so that the operator can never

look directly at the light spot.

Use with one objective

1. Adjust the UV protection screen laterally

using the arm.

2. Unscrew the hexagon-head screw.

3. Adjust the UV protection screen using the

arm.

4. Tighten the hexagon-head screw.

5. Fasten the UV protection screen using a

hexagon-head screw to the left or right side
of the microscope carrier.

Leica M-series User Manual 27

Objective Nosepiece – Assembly

Preparations

Hold the objectives firmly during

•

assembly and disassembly so that they

do not fall onto the stage plate.

ρ

Move the drive housing all the way upwards
and remove the optics carrier, if the carrier
has already been installed.

Assembly

1. Remove the transport anchor from the
objective nosepiece.

2. Rotate the moving part by 90° and attach
the objective nosepiece to the drive hous­ing from the front (!). Screw the objective
nosepiece firmly into place.

3. Unscrew the three Phillips screws on the
objective mount of the optics carrier and
remove the intermediate ring.

4. Screw the optics carrier onto the objective
nosepiece.

5. Screw both objectives onto the objective
nosepiece. It makes no difference which
position an objective occupies.

6. Unscrew the locking screws on both sides
of the objective nosepiece.

You can now adjust the parfocality (see instruc­tions on the next page).

Leica M-series User Manual 28

Objective Nosepiece – Adjusting Parfocality

The following procedure only has to be carried
out once. Afterwards, both objectives are parfo­cal, meaning that the specimen remains in focus
when the objective nosepiece is rotated.

This procedure must be repeated if you replace
either of the two objectives with another.

The following example assumes the combi­nation of the 1× and 2× planapochromats. If
you are using another objective combination,
replace the 2× objective in the description with
the objective with the stronger magnification.

Preparation

ρ

Open the iris diaphragm.

ρ

Set the dioptric correction of the eyepieces
to "0".

Adjustment

1. Rotate the 2× objective into the beam path
and set it to the lowest magnification.

2. Focus on the specimen.

3. Rotate the 1× objective into the beam
path.

4. Turn the objective on the thread in both
directions until the specimen appears
sharp.

5. Toggle to the 2× objective.

6. Select the strongest magnification and
refocus until the specimen appears abso­lutely sharp.

7. Toggle to the 1× objective.

8. Turn the objective on the thread in both
directions until the specimen appears
absolutely sharp.

By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
with the other objective. If it is not parfocal,
repeat the procedure.

9. Tighten the locking screws.

Leica M-series User Manual 29

Transmitted-light Base TL ST

Unpacking the base

The base is delivered with the adapter plate
installed. Make sure the instruments are
unpacked on a flat, sufficiently dimensioned,
and non-slip surface.

Focusing drive and column

1. Unscrew the extension plate from the base
using the Allen key provided.

2. Attach your focusing drive column from
below using the four hexagon-head
screws.

3. Re-attach the adapter plate to its origi­nal position using the six hexagon-head
screws.

Leica M-series User Manual 30